Recombinant Sj16 from Schistosoma japonicum contains a functional N-terminal nuclear localization signal necessary for nuclear translocation in dendritic cells and IL-10 production
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- 英文论文题名：Recombinant Sj16 from Schistosoma japonicum contains a functional N-terminal nuclear localization signal necessary for nuclear translocation in dendritic cells and IL-10 production
- 论文作者：Xi Sun ; Fan Yang ; Jia Shen ; Zhen Liu ; Jinyi Liang ; Huanqin Zheng ; Mingqiu Fung ; Zhiyue Lv ; Zhongdao Wu
- 英文论文作者：Xi Sun ; Fan Yang ; Jia Shen ; Zhen Liu ; Jinyi Liang ; Huanqin Zheng ; Mingqiu Fung ; Zhiyue Lv ; Zhongdao Wu ; Department of Parasitology ; Zhongshan School of Medicine ; Sun Yat-sen University ; Key Laboratory for Tropical Diseases Control ; the Ministry of Education ; Department of Biotherapy ; The 3rd Affiliated Hospital of Sun Yat-sen University ; Department of Biology ; Chinese University of Hongkong
- 年：2016
- 作者机构：Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University;Key Laboratory for Tropical Diseases Control, the Ministry of Education;Department of Biotherapy, The 3rd Affiliated Hospital of Sun Yat-sen University;Department of Biology, Chinese University of Hongkong;
- 英文论文关键词：dendritic cell ; rSj16 ; immuno-modulation ; nuclear localization signal ; IL-10
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：R383.24
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：327k
- 原文格式：D
- 会议级别：全国
摘要
Background: Sj16, a 16 k Da protein derived from Schistosoma japonicum, has been identified as an immune modulation molecule, but the mechanisms of modulation of immune responses are poor known. In this report, we want to reveal the immunity mechanism of regulation of host immune responses by r Sj16 and illuminate the molecule mechanism of immune evasion of Schistosoma japonicum, Methods: Purified r Sj16 protein was produced from E.coil. Using the antibody blocking experiments and knockout mouse model, the effects of r Sj16 and r Sj16 mutants on the biology of dendritic cells were assessed by examining DC maturation, cytokine production and expression of surface markers by flow cytometry and ELISA. Statistical significance was determined by Two-way ANOVA. Results: We investigated the influence of r Sj16 on LPS-induced dendritic cells and found that r Sj16 could significantly stimulate IL-10 production and inhibit LPS-induced BMDC maturation in a dose-dependent manner. Using antibody neutralization experiment and IL-10-deficent(IL-10 Knockout) mice, we further confirmed that the inhibitory effect of r Sj16 on LPS-induced BMDCs is due to its induction of IL-10 production. To understand how r Sj16 induce the production of IL-10, we analyzed the sequence and revealed two potential nuclear localization signal(NLS) of Sj16. Further studies showed that N-terminal NLS(NLS1) is both necessary and sufficient for translocation of r Sj16 to the BMDC's nucleus and important for subsequent induction of IL-10 production and inhibition of BMDCs maturation by r Sj16. Conclusion: Our results suggest that r Sj16 enter into the nuclear of host cells using the NLS1 and promote the production of IL-10 which mediates the inhibitory effect of r Sj16 on LPS-induced BMDCS.
Background: Sj16, a 16 k Da protein derived from Schistosoma japonicum, has been identified as an immune modulation molecule, but the mechanisms of modulation of immune responses are poor known. In this report, we want to reveal the immunity mechanism of regulation of host immune responses by r Sj16 and illuminate the molecule mechanism of immune evasion of Schistosoma japonicum, Methods: Purified r Sj16 protein was produced from E.coil. Using the antibody blocking experiments and knockout mouse model, the effects of r Sj16 and r Sj16 mutants on the biology of dendritic cells were assessed by examining DC maturation, cytokine production and expression of surface markers by flow cytometry and ELISA. Statistical significance was determined by Two-way ANOVA. Results: We investigated the influence of r Sj16 on LPS-induced dendritic cells and found that r Sj16 could significantly stimulate IL-10 production and inhibit LPS-induced BMDC maturation in a dose-dependent manner. Using antibody neutralization experiment and IL-10-deficent(IL-10 Knockout) mice, we further confirmed that the inhibitory effect of r Sj16 on LPS-induced BMDCs is due to its induction of IL-10 production. To understand how r Sj16 induce the production of IL-10, we analyzed the sequence and revealed two potential nuclear localization signal(NLS) of Sj16. Further studies showed that N-terminal NLS(NLS1) is both necessary and sufficient for translocation of r Sj16 to the BMDC's nucleus and important for subsequent induction of IL-10 production and inhibition of BMDCs maturation by r Sj16. Conclusion: Our results suggest that r Sj16 enter into the nuclear of host cells using the NLS1 and promote the production of IL-10 which mediates the inhibitory effect of r Sj16 on LPS-induced BMDCS.
Background: Sj16, a 16 k Da protein derived from Schistosoma japonicum, has been identified as an immune modulation molecule, but the mechanisms of modulation of immune responses are poor known. In this report, we want to reveal the immunity mechanism of regulation of host immune responses by r Sj16 and illuminate the molecule mechanism of immune evasion of Schistosoma japonicum, Methods: Purified r Sj16 protein was produced from E.coil. Using the antibody blocking experiments and knockout mouse model, the effects of r Sj16 and r Sj16 mutants on the biology of dendritic cells were assessed by examining DC maturation, cytokine production and expression of surface markers by flow cytometry and ELISA. Statistical significance was determined by Two-way ANOVA. Results: We investigated the influence of r Sj16 on LPS-induced dendritic cells and found that r Sj16 could significantly stimulate IL-10 production and inhibit LPS-induced BMDC maturation in a dose-dependent manner. Using antibody neutralization experiment and IL-10-deficent(IL-10 Knockout) mice, we further confirmed that the inhibitory effect of r Sj16 on LPS-induced BMDCs is due to its induction of IL-10 production. To understand how r Sj16 induce the production of IL-10, we analyzed the sequence and revealed two potential nuclear localization signal(NLS) of Sj16. Further studies showed that N-terminal NLS(NLS1) is both necessary and sufficient for translocation of r Sj16 to the BMDC's nucleus and important for subsequent induction of IL-10 production and inhibition of BMDCs maturation by r Sj16. Conclusion: Our results suggest that r Sj16 enter into the nuclear of host cells using the NLS1 and promote the production of IL-10 which mediates the inhibitory effect of r Sj16 on LPS-induced BMDCS.
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