脉冲电磁场对大鼠坐骨神经损伤后转化生长因子β1表达的影响
摘要
目的:探讨坐骨神经损伤后TGF-β1表达变化及脉冲电磁场对TGF-β1表达的影响。
方法;采用大鼠坐骨神经损伤动物模型,将52只SD大鼠随机分成正常组4只,治疗组、夹伤组,对照组每组16只,根据实验动物的手术时间和处死取材时间的不同,组内再随机分为术后1d,3d,7d,14d等4个时相观察点,各4只大鼠。夹伤组:夹伤右侧坐骨神经,予以空白电磁场治疗(磁场强度为0);治疗组:夹伤右侧坐骨神经,予以脉冲电磁场治疗;对照组:切开暴露坐骨神经后即缝合,不给予任何其他处理;正常组:不给与任何干预,保持同样饲养条件至试验结束。
以坐骨神经功能指数(SFI)评定功能状况,HE染色观察组织学改变,免疫组化技术检测TGF-β1的表达,用计算机图像自动处理系统进行定量分析研究。
结果:1.治疗组SFI值与损伤组相比无统计学意义(P＞0.05)。
2:HE染色光镜下观察:治疗组神经变性程度较损伤组严重。
3:TGF-β1在损伤神经中表达持续增高,在本次试验终点达到最高值,治疗组与损伤组相比,TGF-β1表达增高,术后1d,3d,7d,14d有统计学意义(P＜0.05)。
结论:1 PEMFs对神经损伤早期功能的恢复无明显作用。
2 PEMFs能加速损伤早期远侧神经段的Wallerian变性进程。
3 TGF-β1在损伤神经中表达增高,在本次观察的时间点内呈逐渐升高趋势,PEMFs可使该上升曲线左移,说明PEMFs可促进周围神经损伤后TGF-β1的表达,提示PEMFs对周围神经的作用可能通过TGF-β1来实现。
Objective: To observe the change of transforming growth factor beta expression after rat sciatic nerve crushed injury and TGF-β1 and the relationship of PEMFs and TGF-β1.
Methods: 52 SD rats were divided into four groups: injury group A、PEMFs theropy group B、control group and nomal group D.The sciatic nerve were crushed with a special clamp.Group A were treated with PEMFs exposure and group B with sham explosure after the operation .Group C only exposed the sciatic nerve and group D was not treated any treatment .The histology and immunohistochemistry examination were performed respectively after 1 ,3 ,7,14 days.
Result: 1 :The SFI show no difference comparing group A and group B(P> 0.05).
2: The expression of TGF-β1 increased during the process of nerve regeneration, and reached the maximum 14days after nerve injury. There were distinct difference between group A and group C(P<0.05).
3: The expression of TGF-β1 show distinct difference comparing group A with the group B on 1,3,7,14 days after the operation . PEMFs benefited the expression of TGF-β1 during nerve regeneration (P<0.05).
Conclusion: The PEMFs didn't have obvious effect on the recovery of sciatic nerve function . The PEMFs participate in the pathoiogic process of peripheral nerve damage . and PEMFs promotes the expression of TGF-β1 during scitic nerve regeneration.
方法;采用大鼠坐骨神经损伤动物模型,将52只SD大鼠随机分成正常组4只,治疗组、夹伤组,对照组每组16只,根据实验动物的手术时间和处死取材时间的不同,组内再随机分为术后1d,3d,7d,14d等4个时相观察点,各4只大鼠。夹伤组:夹伤右侧坐骨神经,予以空白电磁场治疗(磁场强度为0);治疗组:夹伤右侧坐骨神经,予以脉冲电磁场治疗;对照组:切开暴露坐骨神经后即缝合,不给予任何其他处理;正常组:不给与任何干预,保持同样饲养条件至试验结束。
以坐骨神经功能指数(SFI)评定功能状况,HE染色观察组织学改变,免疫组化技术检测TGF-β1的表达,用计算机图像自动处理系统进行定量分析研究。
结果:1.治疗组SFI值与损伤组相比无统计学意义(P＞0.05)。
2:HE染色光镜下观察:治疗组神经变性程度较损伤组严重。
3:TGF-β1在损伤神经中表达持续增高,在本次试验终点达到最高值,治疗组与损伤组相比,TGF-β1表达增高,术后1d,3d,7d,14d有统计学意义(P＜0.05)。
结论:1 PEMFs对神经损伤早期功能的恢复无明显作用。
2 PEMFs能加速损伤早期远侧神经段的Wallerian变性进程。
3 TGF-β1在损伤神经中表达增高,在本次观察的时间点内呈逐渐升高趋势,PEMFs可使该上升曲线左移,说明PEMFs可促进周围神经损伤后TGF-β1的表达,提示PEMFs对周围神经的作用可能通过TGF-β1来实现。
Objective: To observe the change of transforming growth factor beta expression after rat sciatic nerve crushed injury and TGF-β1 and the relationship of PEMFs and TGF-β1.
Methods: 52 SD rats were divided into four groups: injury group A、PEMFs theropy group B、control group and nomal group D.The sciatic nerve were crushed with a special clamp.Group A were treated with PEMFs exposure and group B with sham explosure after the operation .Group C only exposed the sciatic nerve and group D was not treated any treatment .The histology and immunohistochemistry examination were performed respectively after 1 ,3 ,7,14 days.
Result: 1 :The SFI show no difference comparing group A and group B(P> 0.05).
2: The expression of TGF-β1 increased during the process of nerve regeneration, and reached the maximum 14days after nerve injury. There were distinct difference between group A and group C(P<0.05).
3: The expression of TGF-β1 show distinct difference comparing group A with the group B on 1,3,7,14 days after the operation . PEMFs benefited the expression of TGF-β1 during nerve regeneration (P<0.05).
Conclusion: The PEMFs didn't have obvious effect on the recovery of sciatic nerve function . The PEMFs participate in the pathoiogic process of peripheral nerve damage . and PEMFs promotes the expression of TGF-β1 during scitic nerve regeneration.
引文
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[1]Kiefer R,Streit WJ,Toyka KV,et,al.Transforming growth factor-beta 1:a lesion-associated cytokine of the nervous system.Dev Neurosci,1995,13(3-4):331-339
[2]Stark B,Carlstedt T,Risling M.Distribution of TGF-beta,the TGF-beta type Ⅰ receptor and the R-Ⅱ receptor in peripheral nerves and mechanoreceptors;observations on changes after traumatic injury.Brain Res,2001,913(1):47-56
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[5]Rufer M,Flanders K,Unsicker K.Presence and regulation of transforming growth factor beta mRNA and protein in the normal and lesioned rat sciatic nerve.Neurosci Res,1994,39(4):412-423
[6]Scherer SS,Kamholz J,Jakowlew SB.Axons modulate the expression of transforming growth factor-betas in Schwann cells.Glia,1993,8(4):265-276
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[9]董震,成效敏,顾玉东.坐骨神经卡压对腰脊髓神经的影响.中国修复重建外科杂志,1998,12(2):113-116
[10]DeLeo JA,Colburn RW,Rickman AJ.Cytokine and growth factor immunohistochemical spinal profiles in two animal models of mononeuropathy.Brain Res,1997,759(1):50-57
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[2]王迅,刘兴波,王泓杰.雌二醇对大鼠坐骨神经损伤的保护.中国临床康复,2006,10(26):101-102
[3]史淑贤,程纯,陈梦玲,等p27kip1和Skp2在损伤后大鼠坐骨神经中的表达变化.神经解剖学杂志,2006,23(1):20-24
[4]郭小伟,齐志远,宋健.脉冲电磁场对坚强内固定后兔胫骨生物力学的影响.中医正骨,2001,13(4):9-10
[5]国永智,李文革,齐斌.脉沖电磁场对家兔软骨骨折愈合的影响.中国急救医学,2000,20(6):349
[6]夏群,冯远明,金鸿宾.脉冲电磁场对骨折愈合及钙磷代谢的影响.中国骨伤,1999,12(4):3-4
[7]柯丽,陈璐璐,涂意辉,等.复合脉冲电磁场对去卵巢大鼠骨质疏松预防作用的研究.中国骨质疏松杂志,2001,7(3):224-227
[8]Shen NJ,Zhu JK..Application of sciatic functional index in nerve functional assessment.Microsurgery,1995,16(8):552-555
[9]Hare GMT,Evans PJ,Mackinnon SE,et al.Walking track analysis:A longterm assessment of peripheral nerve recovery.Plast Reconstr Surg,1992,89:251-258
[10]Barbin G,Manthorpe M,Varson S.Purification of the chick eye neurothophic factor.Neurochem,1984,43(5):1486-1488
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