茶多酚纳米粒复合明胶膜的研究
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摘要
本文系统研究了成膜基质─鮰鱼鱼皮明胶的提取工艺、茶多酚纳米粒的制备工艺、茶多酚纳米粒对明胶膜的结构与性质的影响以及茶多酚纳米粒复合明胶膜中茶多酚的释放动力学。
首先研究了制备鮰鱼鱼皮明胶的工艺,并对明胶性质作了分析。采用酸溶胀、打浆、溶解、沉淀的方法得到制备鮰鱼鱼皮明胶的中间产物─鮰鱼鱼皮胶原纤维;在45°C、pH7.0条件下,对鮰鱼鱼皮胶原纤维经热提胶6h得鮰鱼鱼皮明胶,明胶提取率可达95%以上,明胶溶液胶凝温度与明胶凝胶熔化温度分别为19.6°C、25.2°C。
确定了乙醇注入─超声法制备了茶多酚纳米脂质体悬浮液的最佳工艺为:卵磷脂浓度为1.0 mg/mL,胆固醇/卵磷脂质量比为1:5,Tween80/卵磷脂质量比为4:5,茶多酚添加量为25 mg,乙醇用量为8 mL,水合介质离子强度为0.05 mmol/L,水合时间及水合温度分别为30 min、50 oC,超声破碎后处理(380 W,4 min,1 s开,1 s停),按此工艺制备的茶多酚纳米脂质体悬浮液中颗粒粒径分布均匀,大小约为126 nm,表面电位约为-11.6 mV。以鮰鱼鱼皮明胶为基质,添加茶多酚纳米脂质体悬浮液制备了茶多酚纳米脂质体复合明胶膜。研究发现,茶多酚纳米脂质体通过自身的空间位阻效应以及同明胶分子上的C=O形成的氢键作用,降低了明胶分子之间的氢键交联,改变了明胶膜的性质:增加了明胶膜的厚度、断裂伸长率、透氧系数及对紫外光的吸收能力;降低了明胶膜的拉伸强度、透湿系数、透明度;显著提高了明胶膜的抗氧化能力。
确定了离子交联─超声法制备茶多酚-壳聚糖纳米粒悬浮液的最佳工艺为:壳聚糖浓度为2.0 mg/mL,pH为5.5,TPP/CTS为1:3,茶多酚添加量为25 mg,TPP以注射方式加入,搅拌速度为800 r/min,超声破碎后处理(380 W,4 min,1 s开,1 s停)。按此工艺制备的茶多酚-壳聚糖纳米粒悬浮液颗粒粒径分布均匀,大小约为300 nm,Zeta电位约为44.6 mV。以鮰鱼鱼皮明胶为基质,添加茶多酚-壳聚糖纳米粒悬浮液制备了茶多酚-壳聚糖纳米粒复合明胶膜。研究发现,茶多酚-壳聚糖纳米粒的空间位阻效应以及壳聚糖分子上的羟基与明胶分子上的活性基团形成氢键,干扰了明胶分子之间的氢键作用,影响了明胶膜的性能:增加了明胶膜的厚度,对紫外光的吸收能力以及透湿系数;降低了明胶膜的拉伸强度、断裂伸长率及透氧系数;大大提高了明胶膜的抗氧化能力。
研究了茶多酚-纳米粒悬浮液中的茶多酚在PBS中释放动力学、茶多酚-纳米粒中的茶多酚在明胶膜中释放动力学以及茶多酚-纳米粒复合明胶膜在PBS中释放茶多酚的动力学。结果发现:①在PBS中,茶多酚-纳米粒悬浮液内的茶多酚的释放均符合一级动力学模型,但茶多酚-壳聚糖纳米粒悬浮液内茶多酚的累计释放率高于茶多酚纳米脂质体悬浮液内茶多酚的累计释放率;②在明胶膜中,茶多酚-纳米粒包埋的茶多酚的释放同样符合一级动力学,但茶多酚-纳米粒悬浮液内的茶多酚在PBS中释放的速率要慢很多,且茶多酚-壳聚糖纳米粒内的茶多酚的释放较茶多酚纳米脂质体内茶多酚的释放受到的影响更大;③茶多酚从茶多酚-纳米粒复合明胶膜向PBS中的释放与茶多酚-纳米粒复合明胶膜在PBS中的溶胀、溶蚀有关,茶多酚纳米脂质体复合明胶膜在PBS中快速溶蚀,茶多酚纳米脂质体从复合明胶膜中分散到PBS中释放茶多酚;茶多酚-壳聚糖纳米粒复合明胶膜在PBS中经历了一个先溶胀后溶解的过程,茶多酚先是从茶多酚-壳聚糖纳米粒释放到溶胀的明胶膜内,然后通过水分子渗透剂在膜内形成的扩散通道再释放到PBS中,释放速率较茶多酚纳米脂质体复合明胶膜内茶多酚的释放大大下降。
The extraction method of channel catfish skin gelatin and tea polyphenol loaded nanoparticles was developed. The influence of tea polypheno loaded nanoparticles on gelatin film was evaluated. Furthermore, the release kinetics of tea polyphenol from composite films was investigated.
Gelatin was extracted from channel catfish skin with a novel method and characterized. The skin was allowed to swell in acetic acid, and then was mashed with triturator, dissolved in dilte acid, precipitated by adding NaOH to obtained collagen fibers. Finnally, the collagen fibers were extracted for gelatin in the water of 45°C、pH7.0 within 6 hours. It was found that the yield of gelatin had exceeded 95%, the gelation temperature of gelatin solution and gelatin gel melting point were 19.6°C、25.2°C, respectively.
Tea polyphenols nanoliposomes (TP-L) were prepared with by ethanol injection- sonication method. Higher encapsulating efficiency of 25 mg tea polyphenols was obtained with lecithin concentraction of 1.0 mg/mL, cholesterol to lecithin mass ratio of 1:5, Tween 80 and lipid to the molar ratio of 4:5, hydration temperature of 50°C, hydration time of 30 min and 50 mL of PBS (0.05 mol/L, pH7.0). The particles of nanoliposomes were about 120 nm and zeta potential was in the range of -10 and -20 mV. With the incorporation of TP-L, a kind of composite films (TP-L F) based on channel catfish skin gelatin was obtained. Due to the steric effects from TP-L and the formation of hydrogen between TP-L and gelatin molecules, the hydrogen bonds between the gelatin-gelatin stabilizing the network of gelatin film had been reduced, consequently, the properties of the composite film had been changed. With a respect, antioxidative activity of the film had been enhanced siginficantlly.
Technical route of preparation for tea polyphenols loaded chitosan- sodium tripolyphosphate nanoparticles (TP-CTS-TPP) were confirmed. The optimum technical parameters with highest encapsulating efficiency were: chitosan concentraction of 2.0 mg/mL, pH5.5, TPP to chitosan mass ratio of 1:3, TP of 25 mg, time of 30 min. The resulted nanoparticels was about 300 nm and zeta potential was in the range of 40 and 50 mV. The composite gelatin film (TP-CTS-TPP F) incorporated TP-CTS-TPP was prepared and its antioxidative activity and properties were investigated. It showed, the incorporation of TP-CTS-TPP interfered with hydrogen forming between gelatin molecules, owing to the steric effects from TP-CTS-TPP and the formation of hydrogen including interaction of amino-group on chitosan with amino-group on gelatin and interaction of hydroxyl-group on chitosan with amino-group on gelatin. So, the properties of composite film were modified, but its antioxidative ability was improved to a large extent.
Finally, the release kinetics of TP-L and TP-CTS-TPP in PBS, the release kinetics of TP-L and TP-CTS-TPP in gelatin films as well as the release kinetics of TP-L F and TP-CTS-TPP F in PBS were characterized. The first, the release kinetics of TP-L and TP-CTS-TPP in PBS fitted the first release kinetic model well; however, the cumulative release rate of TP-L was higher than that of TP-CTS-TPP. The second, the release kinetics of TP-L and TP-CTS-TPP in gelatin films also were consistent with the first release kinetic model; however, the release rate of TP-L and TP-CTS-TPP were lower than their in PBS. The third, the release characteristicas of TP from TP-L F and TP-CTS-TPP F in PBS were related to the swelling ability and dissolutions of the composite films. TP-L F corroded in PBS quickly, and TP was released from TP-L dispersed in PBS. At the first stage, the TP-CTS-TPP F in PBS happened to limited dissolution and to became hydrogel, TP released from TP-CTS-TPP fixed in hydrogel and then diffused from hydrogel to PBS through the channel formed by water in the course of swelling procdure.
首先研究了制备鮰鱼鱼皮明胶的工艺,并对明胶性质作了分析。采用酸溶胀、打浆、溶解、沉淀的方法得到制备鮰鱼鱼皮明胶的中间产物─鮰鱼鱼皮胶原纤维;在45°C、pH7.0条件下,对鮰鱼鱼皮胶原纤维经热提胶6h得鮰鱼鱼皮明胶,明胶提取率可达95%以上,明胶溶液胶凝温度与明胶凝胶熔化温度分别为19.6°C、25.2°C。
确定了乙醇注入─超声法制备了茶多酚纳米脂质体悬浮液的最佳工艺为:卵磷脂浓度为1.0 mg/mL,胆固醇/卵磷脂质量比为1:5,Tween80/卵磷脂质量比为4:5,茶多酚添加量为25 mg,乙醇用量为8 mL,水合介质离子强度为0.05 mmol/L,水合时间及水合温度分别为30 min、50 oC,超声破碎后处理(380 W,4 min,1 s开,1 s停),按此工艺制备的茶多酚纳米脂质体悬浮液中颗粒粒径分布均匀,大小约为126 nm,表面电位约为-11.6 mV。以鮰鱼鱼皮明胶为基质,添加茶多酚纳米脂质体悬浮液制备了茶多酚纳米脂质体复合明胶膜。研究发现,茶多酚纳米脂质体通过自身的空间位阻效应以及同明胶分子上的C=O形成的氢键作用,降低了明胶分子之间的氢键交联,改变了明胶膜的性质:增加了明胶膜的厚度、断裂伸长率、透氧系数及对紫外光的吸收能力;降低了明胶膜的拉伸强度、透湿系数、透明度;显著提高了明胶膜的抗氧化能力。
确定了离子交联─超声法制备茶多酚-壳聚糖纳米粒悬浮液的最佳工艺为:壳聚糖浓度为2.0 mg/mL,pH为5.5,TPP/CTS为1:3,茶多酚添加量为25 mg,TPP以注射方式加入,搅拌速度为800 r/min,超声破碎后处理(380 W,4 min,1 s开,1 s停)。按此工艺制备的茶多酚-壳聚糖纳米粒悬浮液颗粒粒径分布均匀,大小约为300 nm,Zeta电位约为44.6 mV。以鮰鱼鱼皮明胶为基质,添加茶多酚-壳聚糖纳米粒悬浮液制备了茶多酚-壳聚糖纳米粒复合明胶膜。研究发现,茶多酚-壳聚糖纳米粒的空间位阻效应以及壳聚糖分子上的羟基与明胶分子上的活性基团形成氢键,干扰了明胶分子之间的氢键作用,影响了明胶膜的性能:增加了明胶膜的厚度,对紫外光的吸收能力以及透湿系数;降低了明胶膜的拉伸强度、断裂伸长率及透氧系数;大大提高了明胶膜的抗氧化能力。
研究了茶多酚-纳米粒悬浮液中的茶多酚在PBS中释放动力学、茶多酚-纳米粒中的茶多酚在明胶膜中释放动力学以及茶多酚-纳米粒复合明胶膜在PBS中释放茶多酚的动力学。结果发现:①在PBS中,茶多酚-纳米粒悬浮液内的茶多酚的释放均符合一级动力学模型,但茶多酚-壳聚糖纳米粒悬浮液内茶多酚的累计释放率高于茶多酚纳米脂质体悬浮液内茶多酚的累计释放率;②在明胶膜中,茶多酚-纳米粒包埋的茶多酚的释放同样符合一级动力学,但茶多酚-纳米粒悬浮液内的茶多酚在PBS中释放的速率要慢很多,且茶多酚-壳聚糖纳米粒内的茶多酚的释放较茶多酚纳米脂质体内茶多酚的释放受到的影响更大;③茶多酚从茶多酚-纳米粒复合明胶膜向PBS中的释放与茶多酚-纳米粒复合明胶膜在PBS中的溶胀、溶蚀有关,茶多酚纳米脂质体复合明胶膜在PBS中快速溶蚀,茶多酚纳米脂质体从复合明胶膜中分散到PBS中释放茶多酚;茶多酚-壳聚糖纳米粒复合明胶膜在PBS中经历了一个先溶胀后溶解的过程,茶多酚先是从茶多酚-壳聚糖纳米粒释放到溶胀的明胶膜内,然后通过水分子渗透剂在膜内形成的扩散通道再释放到PBS中,释放速率较茶多酚纳米脂质体复合明胶膜内茶多酚的释放大大下降。
The extraction method of channel catfish skin gelatin and tea polyphenol loaded nanoparticles was developed. The influence of tea polypheno loaded nanoparticles on gelatin film was evaluated. Furthermore, the release kinetics of tea polyphenol from composite films was investigated.
Gelatin was extracted from channel catfish skin with a novel method and characterized. The skin was allowed to swell in acetic acid, and then was mashed with triturator, dissolved in dilte acid, precipitated by adding NaOH to obtained collagen fibers. Finnally, the collagen fibers were extracted for gelatin in the water of 45°C、pH7.0 within 6 hours. It was found that the yield of gelatin had exceeded 95%, the gelation temperature of gelatin solution and gelatin gel melting point were 19.6°C、25.2°C, respectively.
Tea polyphenols nanoliposomes (TP-L) were prepared with by ethanol injection- sonication method. Higher encapsulating efficiency of 25 mg tea polyphenols was obtained with lecithin concentraction of 1.0 mg/mL, cholesterol to lecithin mass ratio of 1:5, Tween 80 and lipid to the molar ratio of 4:5, hydration temperature of 50°C, hydration time of 30 min and 50 mL of PBS (0.05 mol/L, pH7.0). The particles of nanoliposomes were about 120 nm and zeta potential was in the range of -10 and -20 mV. With the incorporation of TP-L, a kind of composite films (TP-L F) based on channel catfish skin gelatin was obtained. Due to the steric effects from TP-L and the formation of hydrogen between TP-L and gelatin molecules, the hydrogen bonds between the gelatin-gelatin stabilizing the network of gelatin film had been reduced, consequently, the properties of the composite film had been changed. With a respect, antioxidative activity of the film had been enhanced siginficantlly.
Technical route of preparation for tea polyphenols loaded chitosan- sodium tripolyphosphate nanoparticles (TP-CTS-TPP) were confirmed. The optimum technical parameters with highest encapsulating efficiency were: chitosan concentraction of 2.0 mg/mL, pH5.5, TPP to chitosan mass ratio of 1:3, TP of 25 mg, time of 30 min. The resulted nanoparticels was about 300 nm and zeta potential was in the range of 40 and 50 mV. The composite gelatin film (TP-CTS-TPP F) incorporated TP-CTS-TPP was prepared and its antioxidative activity and properties were investigated. It showed, the incorporation of TP-CTS-TPP interfered with hydrogen forming between gelatin molecules, owing to the steric effects from TP-CTS-TPP and the formation of hydrogen including interaction of amino-group on chitosan with amino-group on gelatin and interaction of hydroxyl-group on chitosan with amino-group on gelatin. So, the properties of composite film were modified, but its antioxidative ability was improved to a large extent.
Finally, the release kinetics of TP-L and TP-CTS-TPP in PBS, the release kinetics of TP-L and TP-CTS-TPP in gelatin films as well as the release kinetics of TP-L F and TP-CTS-TPP F in PBS were characterized. The first, the release kinetics of TP-L and TP-CTS-TPP in PBS fitted the first release kinetic model well; however, the cumulative release rate of TP-L was higher than that of TP-CTS-TPP. The second, the release kinetics of TP-L and TP-CTS-TPP in gelatin films also were consistent with the first release kinetic model; however, the release rate of TP-L and TP-CTS-TPP were lower than their in PBS. The third, the release characteristicas of TP from TP-L F and TP-CTS-TPP F in PBS were related to the swelling ability and dissolutions of the composite films. TP-L F corroded in PBS quickly, and TP was released from TP-L dispersed in PBS. At the first stage, the TP-CTS-TPP F in PBS happened to limited dissolution and to became hydrogel, TP released from TP-CTS-TPP fixed in hydrogel and then diffused from hydrogel to PBS through the channel formed by water in the course of swelling procdure.
引文
1.施惠群,邹胜祥,许文苡.水产动物明胶[M].北京:农业出版社,1982
2. Ward A G, Courts A.明胶的科学与工艺学[M].李文渊等译,彭必先校.北京:中国轻工业出版社, 1982
3.王颖,陈丽娟,彭必先.鱼明胶[J].明胶科学与工艺学,1997,17(3): 113-123
4.Karim A A, Rajeev Bhat. Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins[J]. Food Hydrocolloids, 2009, 23: 563-576
5.Djagny K B, Wang Z, Xu S Y. Gelatin: a valuable protein for food and pharmaceutical industries: review[J]. Critical Reviews in Food Science and Nutrition, 2001, 41(6): 481-492
6.《明胶生产工艺及设备》编写组.明胶生产工艺及设备[M].北京:中国轻工业出版社, 1986
7.上海明胶厂.皮胶明胶的生产[M].上海:上海人民出版社, 1973
8.苏州化工厂.骨胶明胶的生产[M].上海:上海人民出版社, 1977
9.任乃容.我国明胶科研十年简要回顾(二)(1981～1990)[J].明胶科学与技术, 1995, 15(3): 9-14
10.徐润,梁庆华.明胶的生产及应用技术[M].北京:中国轻工业出版社, 1997
11.Zhou P, Regenstein J M. Optimization of extraction conditions for Pollock skin gelatin[J]. Journal of Food Science, 2004, 69(5): C392-C398
12.Ladislaus M K, Yan X, Yao W L, et al. Optimization of gelatine extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology[J]. Bioresource Technology, 2007, 98: 3338-3343
13.Gime′neza B, Turnayb J, Lizarbeb M A, et al. Use of lactic acid for extraction of fish skin gelatin[J]. Food Hydrocolloids, 2005, 19: 941-950
14.刘小玲,许时婴.从鸡骨中制取明胶的加工工艺[J].食品与发酵工业,2004,30(10): 48-53
15.刘海英.斑点差尾鮰鱼(channel catfish)皮胶原蛋白及胶原多肽的研究[D]:[博士学位论文].江苏:江南大学, 2007
16.刘丽娜.鮰鱼皮明胶的制备及其功能性质研究[D]:[硕士学位论文].江苏:江南大学, 2008
17.Cho S M, Kwak K S, Park D C, et al. Processing optimization and functional properties of gelatin from shark (Isurus oxyrinchus) cartilage [J]. Food Hydrocolloids, 2004, 18: 573-579
18.张峰.酶法制备明胶的研究[D]:[硕士研究生学位论文].北京:北京化工大学, 2002
19.陈秀金,侯颖,张敏,等. 2709碱性蛋白酶水解脱铬革屑的研究[J].中国皮革, 2007, 36(19): 47-50
20.侯国泉,王璋.酶法从猪皮制备食用明胶工艺的研究[J].无锡轻工业学院学报, 1992, 11(1): 10-20
21.韩应昌,许时婴.酶法制取骨照相明胶工艺的研究[J].无锡轻工业学院学报, 1993, 12(1): 1-13
22.安广杰,徐清萍. Neutrase蛋白酶降解骨胶原蛋白制备水解明胶[J].食品与机械, 2007, 23(3): 39-40
23.魏立新,杜玉枝,王安柱,等.碱性蛋白酶降解骨胶原制备食品明胶工艺[J].安徽农业科学, 2007, 35(20): 6235-6239
24.安广杰,王璋,许时婴.利用Alcalase蛋白酶生产牛骨水解明胶[J].食品与发酵工业,2004, 30(3): 52-55
25.Nalinanon S, Benjakul, Visessanguan W, et al. Improvement of gelatin extraction from bigeye snapper skin using pepsin-aided process in combination with protease inhibitor [J]. Food Hydrocolloids, 2008, 22: 615-622
26.陈丽娟,王颖,彭必先.胰蛋白酶降解骨胶原与明胶组份含量之间的关系[J].感光科学与光化学,1998, 16(3): 258-263
27.Taylor M M, Cabeza L F, Marmer W N, et al. Use of tryptic enzyme preparations in treatment of chrome shavings [J]. Journal of the American Leather Chemists Association, 2000, 95(7): 243
28.吕坪,杜玉枝,魏立新.酶法制备明胶的研究进展[J].明胶科学与技术, 2006, 26(4): 170-176
29.Gómez-Guillén M C, Pérez-Mateos J, Gómez-Estaca E, et al. Fish gelatin: a renewable material for developing active biodegradable films[J]. Trends in Food Science & Technology, 2009, 20: 3-16
30.Gómez-Guillén M C, Turnay J, Fernandez-Diaz M D, et al. Structural and physical properties of gelatin extracted from different marine species: a comparative study[J]. Food Hydrocolloids, 2002, 16: 25-34
31.Leuenberger B H. Investigation of viscosity and gelatin properties of different mammalian and fish gelatins[J]. Food Hydrocolloids, 1991, 5: 353-356
32.Eastoe J E, Leach A A. Chemical constitution of gelatin[M]. London: Academic Press, 1977
33.史京京,陈丽娟,王颖,等.胶冻强度与明胶α组份关系的研究[J].感光科学与光化学, 2002, 20(6): 462-467
34.Young S, Wong M, Tabata Y, et al. Gelatin as a delivery vehicle for the controlled release of bioactive molecules[J]. Journal of Controlled Release, 2005, 109: 256-274
35.Karim A A, Bhat R. Gelatin alternatives for the food industry: Recent developments, challenges and prospects[J]. Trends in Food Science & Technology, 2008, 19: 644-656
36.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
37.Tharanathan RN. Biodegradable films and composite coatings: Past, present and future[J]. Trends in Food Science and Technology, 2003, 14: 71-78
38.曹娜,符玉华,贺军辉.明胶膜的性能研究进展[J].高分子通报, 2007, 8: 1-6
39.Deng C M, He L Z, Zhao M, et al. Biological properties of the chitosan-gelatin sponge wound dressing[J]. Carbohydrate Polymers, 2007, 69: 583-589
40.黄志,许时婴.利用猪胶皮制作可食性胶元人造肠衣的研究[J].食品与发酵工业, 1990, 5: 9-17
41.Gennadios A, Hanna M A, Kurth LB. Application of edible coating on meats, poultry and seafoods: A review. LWT, 1997, 30: 337-350
42.Bigi A, Panzavolta S, Rubini K. Relationship between triple-helix content and mechanical properties of gelatin films[J]. Biomaterials, 2004, 25: 5675-5680
43.刘小杰,齐鲁,白立峰.改性明胶膜的制备及其力学性能探讨[J].天津工业大学学报, 2006, 25(2): 19-21, 32
44.Cao N, Yang X M, Fu Y. Effects of various plasticizers on mechanical and water vapor barrier properties of gelatin films[J]. Food Hydrocolloids, 2009, 23: 729-735
45.Bigi A, Cojazzi G, Panzavolta S, et al. Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking[J]. Biomaterials, 2001, 22: 763-768
46.Sazedul Hoque M, Benjakul S, Prodpran T. Effect of heat treatment of film-forming solution on the properties of film from cuttlefish (Sepia pharaonis) skin gelatin[J]. Journal of Food Engineering, 2010, 96: 66-73
47.Chambi H, Grosso C. Edible films produced with gelatin and casein cross-linked with transglutaminase[J]. Food Research International, 2006, 39: 458-466
48.Cao N, Fu Y H, He J H. Mechanical properties of gelatin films cross-linked, respectively, by ferulic acid and tannin acid[J]. Food Hydrocolloids, 2007, 21: 575-584
49.Pranotoa Y, Lee C M, Park H J. Characterizations of fish gelatin films added with gellan andκ-carrageenanL[J]. LWT, 2007, 40: 766-774
50.Bertan L C, Tanada-Palmu P S, Siani A C, et al. Effect of fatty acids and“Brazilian elemi”on composite films based on gelatin[J]. Food Hydrocolloids, 2005, 19: 73-82
51.Cao N, Fu Y H, He J H. Preparation and physical properties of soy protein isolate and gelatin composite films[J]. Food Hydrocolloids, 2007, 21: 1153-1162
52.余家会,杜予民,郑化.壳聚糖明胶共混膜[J].武汉大学学报(自然科学版), 1999, 45(4): 440-444
53.李贵安,苗润才.纳米银明胶复合膜的制作[J].陕西师范大学学报(自然科学版), 1999, 27(2): 37-39
54.Lopez-Rubio A, Gavara R, Lagaron J M. Bioactive packaging: turning foods into healthier foods through biomaterials[J]. Trends in Food Science & Technology, 2006, 17: 567-575
55.Gómez-Estaca J, Bravo L, Gómez-Guillén MC, et al. Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extract[J]. Food Chemistry, 2009, 112: 18-25
56.Gime′nez B, Go′mez-Estaca J, Alema′n A, et al. Improvement of the antioxidant properties of squid skin gelatin films by the addition of hydrolysates from squid gelatin[J]. Food Hydrocolloids, 2009, 23: 1322-1327
57.Jongjareonrak A, Benjakul S, Visessanguanb W, et al. Antioxidative activity and properties of fish skin gelatin films incorporated with BHT andα-tocopherol. Food Hydrocolloids, 2008, 22: 449-458
58.赵铁,杜予民,唐汝培.壳聚糖水杨酸盐明胶共混膜结构表征及其抗菌性[J].分析科学学报, 2002, 18(2): 100-104
59.白立峰,齐鲁,刘小杰.含有奥硝哇纳米囊泡的改性明胶膜释药性能的研究[J].中国新药杂志, 2006, 15(24): 2132-2136
60.高玉香,阎玉华,金中秋.眼用药膜的研制及其性能表征[J].武汉理工大学学报, 2009, 31(16): 54-57
61.张团红,胡小玲,乔吉超,等.纳米胶囊的制备与应用进展[J].材料科学与工程学报, 2007, 25(1): 143-146
62.Letchford K, Burt H. A review of the formation and classification of amphiphilic block copolymer nanoparticulate structures: micelles, nanospheres, nanocapsules and polymersomes[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2007, 65: 259-269
63.Zimmer A, Kreuter J?rg. Microspheres and nanoparticles used in ocular delivery systems[J]. Advanced Drug Delivery Reviews, 1995, 16(1): 61-73
64.Sozer N, Kokini J L. Nanotechnology and its applications in the food sector[J]. Trends in Biotechnology, 2009, 27(2): 82-89
65.Mozafari M R. Commentary: Amphiphiles and their aggregates in basic and applied science. A post-conference thought on nomenclature [J]. Cellular & Molecular Biology Letters, 2005, 10(4): 733–734
66.夏书芹.辅酶Q10纳米脂质体的研究[D]:[博士学位论文].江苏:江南大学, 2007
67.许时婴,张晓鸣,夏书芹,等.微胶囊技术─原理与应用[M].北京:化学工业出版社, 2006
68.曹宁宁,羡菲.脂质体的制备方法及研究进展[J].天津理工学院学报, 2003, 19(1): 30-35
69.Patel V B, Misra A N. Encapsulation and stability of clofazimine liposomes[J]. Journal of Microencapsulation, 1999, 16: 357-367
70.Pons M, Foradada M, Estelrich J. Liposomes obtained by the ethanol injection method[J]. International Journal of Pharmaceutics, 1993, 95: 51-56
71.Xia S Q, Xu S Y, Zhang X M. Optimization in the preparation of coenzyme Q10 nanoliposomes[J]. Journal of Agricultural and Food Chemistry, 2006, 54(17): 6358-6366
72.Bangham A D, Standish M M, Watkins J C. Diffusion of univalent ions across the lamellae of swollen phospholipids[J]. Journal of Molecular Biology, 1965, 13: 238-252
73.Maestrelli F, Gonzalez-Rodriguez M L, Rabasco A M, et al. Effect of preparation technique on theproperties of liposomes encapsulating ketoprofen-cyclodextrin complexes aimed for transdermal delivery[J]. International Journal of Pharmaceutics, 2006, 298: 53-60
74.Lasic D D. Liposomes: from physics to application[M]. Netherlands: Elsevier, 1993
75.Vyas S P, Singh R P, Jain S, et al. Non-ionic surfactant based vesicles (niosomes) for non-invasive topical genetic immunization against hepatitis B[J]. International Journal of Pharmaceutics, 2005, 296: 80-86
76.邓意辉,王鹤东.主动载药与被动载药制备盐酸左氧氟沙星脂质体[J].中国抗生素杂志,2001,26(4):258-260
77.蒋挺大.壳聚糖[M].北京:化学工业出版社, 2007
78.Kosaraju S L, D’ath L, Lawrence A. Preparation and characterisation of chitosan microspheres for antioxidant delivery[J]. Carbohydrate Polymers, 2006, 64: 163-167
79.Harish Prashanth KV and Tharanathan RN, Chitin/chitosan: modifications and their unlimited application potential-an overview[J]. Trends in Food Science and Technology, 2007, 18: 117-131
80.张玮,张学农.壳聚糖纳米粒制备技术研究进展[J].抗感染药学, 2008, 5(2): 65-69
81.Agnihotri S A, Mallikarjuna N N, Aminabhavi T M. Recent advances on chitosan-based micro- and nanoparticles in drug delivery[M]. Journal of Controlled Release, 2004, 100: 5-28
82.Bergera J, Reista M, Mayer J M, et al. Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2004, 57: 19-34
83.徐忻.壳聚糖纳米载体研究[D]:[博士学位论文].吉林:东北师范大学, 2004
84.Zhang S, Wang Y, Herring J L, et al. Characterization of edible film fabricated with channel catfish (Ictalurus punctatus) gelatin extract using selected pretreatment methods[J]. Journal of Food Science, 2007, 72(9): 498-503
85.Li Y Q, Wong H L, Shuhendler A J, et al. Molecular interactions, internal structure and drug release kinetics of rationally developed polymer-lipid hybrid nanoparticles[J]. Journal of Controlled Release, 2008, 128: 60-70
86.Sunil A A, Nadagouda N M, Tejraj M A. Recent advances on chitosan-based micro-and nanoparticles in drug delivery[J]. Journal of Control release, 2004, 100(1): 5-28
87. Pothakamury U R, Barbosa-Ganovas G V. Fundamental aspects of controlled release in foods[J]. Trends in Food Science and Technology, 1995, 6: 397-406
88.Lin M, Meng S, Zhong W, et al. Novel drug-loaded gelatin films and their sustained-release performance[J]. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009, 90B: 939-944
89.Li W P A, Mhando J R. Formulation of sustained-release products: dissolution and diffusion-controlled release from gelatin films[J]. International Journal of Pharmaceutics, 1984, 20(1): 87-98
90.杨贤强,王岳飞,陈留记.茶多酚化学[M].上海:上海科学技术出版社, 2003
91.Friedman M. Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas[J]. Molecular Nutrition & Food Research, 2007, 51: 116-134
92.孟祥,李保国,齐正.茶多酚及其微胶囊化研究进展[J].食品科技, 2006, 11: 37-39
1.蒋挺大.胶原与胶原蛋白[M].北京:化学工业出版社, 2006
2.Vuorio E, Crombrugghe B. The family of collagen genes[J].Annu Review Biochemistry, 1990, 50: 872-873
3.Ward A G, Courts A.明胶的科学与工艺学[M].李文渊等译.北京:中国轻工业出版社, 1982
4.Djagny K B, Wang Z, Xu S Y. Gelatin: a valuable protein for food and pharmaceutical industries: review[J]. Critical Reviews in Food Science and Nutrition, 2001, 41(6): 481-492
5.Karim A A, Rajeev Bhat. Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins[J]. Food Hydrocolloids, 2009, 23: 563-576
6.谢音,屈小英.食品分析[M].北京:科学技术文献出版社, 2006
7.殷明文,南宇梅,王新民.分光光度法测定羟脯氨酸的改进[J].河南医科大学学报, 1994, 29(1): 74-77
8.刘海英.斑点差尾鮰鱼(channel catfish)皮胶原蛋白及胶原多肽的研究[D]:[博士学位论文].江苏:江南大学, 2007
9.侯国泉.酶法从猪皮制备食用明胶工艺的研究[D]:[硕士学位论文].江苏:无锡轻工业大学, 1992
10.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
11.Skierka E, Sadowska M. The influence of different acids and pepsin on the extractability of collagen from the skin of Baltic cod(Gadus morhua)[J]. Food Chemistry, 2007, 105: 1302–1306
12.Liu H Y, Li D, Guo S D. Studies on collagen from the skin of channel catfish(Ictalurus Punctaus)[J]. Food Chemistry, 2007, 101(2): 621-625
13.Zeng S K, Zhang C H, Lin H, et al. Isolation and characterisation of acid-solubilised collagen from the skin of Nile tilapia (Oreochromis niloticus)[J]. Food Chemistry, 2009, 116: 879-883
14.胡胜,李志强,陈敏.皮胶原蛋白的酶法提取及在高附加值领域的应用[J].皮革科学与工程, 2002, 12(5): 38-43
15.Li Y, Li Y W, Du Z L, et al. Comparison of dynamic denaturation temperature of collagen with its static denaturation temperature and the configuration characteristics in collagen denaturation processes[J]. Thermochimica Acta, 2008, 469: 71-76
16.刘小玲,许时婴.从鸡骨中制取明胶的加工工艺[J].食品与发酵工业, 2004, 30(5): 48-53
17.Ko?odziejska I, Skierka E, Sadowska M, et al. Effect of extracting time and temperature on yield of gelatin from different fish offal[J]. Food Chemistry, 2008, 107: 700-706
18.Zeugolis D I, Paul R G, Attenburrow G. Post-self-assembly experimentation on extruded collagen fibres for tissue engineering applications[J]. Acta Biomaterialia, 2008, 4: 1646-1656
19.刘小玲.鸡骨明胶的制备、结构及功能性质研究[D]:[博士学位论文].江苏:江南大学, 2005
20.Usha R, Ramsaami T. Structure and conformation of intramolecularly cross-linked collagen[J]. Colloids and Surfaces B, 2005, 41: 21-24.
21.Muyonga J H, Cole C G B, Duodu K G. Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus)[J]. Food Chemistry, 2004, 85: 81-89
22.Nagai T, Araki Y, Suzuki N. Collagen of the skin of ocellate puffer fish (Takifugu rubripes)[J]. Food Chemistry, 2002, 78: 173-177
23.Burjandze, T. V. Hydroxy-proline content and location in relation to collagen thermal stability. Biopolymers, 1979, 18: 931-936
24.Barbara B, John A M R. The collagen triple-helix Structure[J]. Matrix Biology, 1997, 15(8-9): 545-554
25.Piez K A, Gross J. The amino acid composition of some fish collagens: The relation between composition and structure[J]. Journal of Biological Chemistry, 1960, 235: 995-998
26.John A M R, Jerome W, Allan Bremner. Characterization of type I collagen from skin of Blue Grenadier[J]. Archives of Biochemistry and Biophysics, 1988, 267(2): 497-502
27.阎隆飞,孙之荣.蛋白质分子结构[M].北京:清华大学出版社, 1999
28.刘丽娜.鮰鱼皮明胶的制备及其功能性质研究[D]:[硕士学位论文].江苏:江南大学, 2008
29.Fathima N N, Bose M C, Rao J R, et al. Stabilization of type I collagen against collagenases (type I) and thermal degradation using iron complex[J]. Journal of Inorganic Biochemistry, 2006, 100: 1774-1780
30.Ikoma T, Kobayashi H, Tanaka J, et al. Physical properties of type I collagen extracted from fish scales of Pagrus major and Oreochromis niloticas[J]. International Journal of Biological Macromolecules, 2003, 32: 199-204
31.熊良文,彭必先.圆二色光谱法研究某些助剂对明胶大分子的多极结构的影响[J].感光科学与光化学, 1989, 4: 1-5
32.Gómez-Guillén M C, Turnay J, Fernandez-Diaz M D, et al. Structural and physical properties of gelatin extracted from different marine species: a comparative study[J]. Food Hydrocolloids, 2002, 16: 25-34
33.张俐娜,薛奇,莫志深,等.高分子物理现代研究方法[M].武汉:武汉大学出版社, 1989
34.Gilsenan P M, Ross-Murphy S B. Rheological characterization of gelatins from mammalian and marine sources[J]. Food Hydrocolloids, 2000, 14: 191-19
1.吴韶敏,曹劲松.脂质体技术应用于食品工业的最新研究进展[J].中国油脂,2007,(32):42-46
2.曹宁宁,羡菲.脂质体的制备方法及研究进展[J].天津理工学院学报, 2003, 19(1): 30-35
3.夏书芹.辅酶Q10纳米脂质体的研究[D]:[博士学位论文].江苏:江南大学, 2007
4.范明辉.红景天苷纳米脂质体的研制[D]:[博士学位论文].江苏:江南大学, 2008
5.刘小玲.鸡骨明胶的制备、结构及功能性质研究[D]:[博士学位论文].江苏:江南大学, 2005
6.殷小梅,许时婴.可食茁霉多糖膜的结构与性质研究[J].食品科学, 1998, 19(2): 3-6
7.傅樱花,马长伟.腊肉加工过程中脂质分解及氧化的研究[J].食品科技, 2004, 1: 42-45
8. Fan M H, Xu S Y, Xia S Q, et al. Preparation of salidroside nano-liposomes by Ethanol injection method and in vitro release study[J]. European Food and Research Technology, 2008, 227:167-174
9.Nakayama T, Hashimoto T, Kajiya K, et al. Affinity of polyphenols for lipid bilayers[J]. BioFactors, 2000, 13: 147-151
10.Tanaka T, Kusano R, Kouno I. Synthesis and antioxidant activity of novel amphipathic derivatives of tea polyphenol[J]. Bioorganic & Medicinal Chemistry Letters, 1998, 8: 1801-1806
11.Fang J Y, Lee W R , Shen S C, et al. Effect of liposome encapsulation of tea catechins on their accumulation in basal cell carcinomas[J]. Journal of Dermatological Science, 2006, 42: 101-109
12.Imura T, Otake K, Hashimoto S, et al. Preparation and physicochemical properties of various soybean lecithin liposomes using supercritical reverse phase evaporation method[J]. Colloids and Surfaces B, 2002, 27: 133-140
13.Patel V B, Misra A N. Encapsulation and stability of clofazimine liposomes[J]. Journal of microencapsulation, 1999, 16: 357-367
14.Plessis J, Ramchandran C, Weiner N, et al. The influence of lipid composition and lamellarity of liposomes on the physical stability of liposomes upon storage[J]. International Journal of Pharmaceutics, 1996, 127: 273-278
15.Agarwal R, Katare O P, Vyas S P. Preparation and in vitro evaluation of liposomal/niosomal delivery systems for antipsoriatic drug dithranol[J]. International Journal of Pharmaceutics, 2001, 228: 43-52
16.Jemiota-Rzeminska M, Kruk J, Skowronek M, et al. Location of ubiquinone homologues in liposome membranes studied by fluorescence anisotropy of diphenylhexatriene and trimethylammonium-diphenyl-hexatriene[J]. Chemistry and Physics of Lipids, 1996, 79(1): 55-63
17.Tasi L M, Liu D Z, Chen W Y. Microcalorimetric investigation of the interaction of polysorbate surfactants with unilamellar phosphatidylcholines liposomes[J]. Colloids and Surfaces A 2003, 213: 7-14
18.Takeuchi H, Kojima H, Yamamoto H, et al. Polymer coating of liposomes with a modified polyvinyl alcohol and their systemic circulation and RES uptake in rats[J]. Journal of Controlled Release, 2000, 68: 195-205
19.Carrion C, Domingo J C, Madariaga M A. Preparation of long-circulating immuno-liposomes using PEG-cholesterol conjugates: effect of the spacer arm between PEG and cholesterol on liposomal characteristics[J]. Chemistry and Physics of Lipids, 2001, 113: 97-110
20.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
21.Crosas E, Egea M A, Reig F. Spectroscopic techniques applied to the study of laminin fragments inserted into model membranes[J]. Journal of Colloid and Interface Science, 2006, 295(1): 264-269
22.Biasio A D, Bordi F, Cametti C. Salt-induced aggregation in cationic liposome suspensions[J]. Progress in Colloid and Polymer Science, 2004, 123: 78-82
23.夏书芹,许时婴.辅酶Q10纳米脂质体的制备[J].食品工业科技,2006,2:164-168
24.Schwarz C, Mehnert W, Lucks JS, et al. Solid lipid nanoparticles (SLN) for controlled drug delivery. I.Production, characterization and sterilization[J]. Journal of Controlled Release, 1994, 30: 83-96
25.Silvander M. Steric stabilization of liposomes- a review[J]. Progress in Colloid and Polymer Science, 2002, 120: 35-40
26.Gontard N, Duchez C, Cuq J L, et al. Edible composite films of wheat and lipids: Water vapour permeability and other physical properties[J]. International Journal of Food Science and Technology, 1994, 29: 39-50
27.Bertan L C, Tanada-Palmua P S, Siani A C, et al. Effect of fatty acids and“Brazilian elemi”on composite films based on gelatin[J]. Food Hydrocolloids, 2005, 19: 73-82
28.Jiang M K, Liu S Y, Du X, Physical properties and internal microstructures of films made from catfish skin gelatin and triacetin mixtures[J]. Food Hydrocolloids, 2010, 24: 105-110
29.Yakimets I, Wellner N, Smith A C, et al. Mechanical properties with respect to water content of gelatin films in glassy state. Polymer, 2005, 46: 12577-12585
30.Shi X Y, Ma W Y, Sun C M, et al. The aggregation behavior of collagen in aqueous solution and its property of stabilizing liposomes in vitro[J]. Biomaterials, 2001, 22: 1627-1634
31.杨贤强,王岳飞,陈留记.茶多酚化学[M].上海:上海科学技术出版社, 2003
32.Dwiecki K, Górnas P, Wilk A, et al. Spectroscopic studies of D-α-tocopherol concentration-induced transformation in egg phosphatidylcholne vesicles[J]. Cellular Molecular Biology Letters, 2007, 12(1): 51-69
33.谢晶曦,常俊标.红外光谱-在有机化学和药物化学中的应用[M].北京:科学出版社,2001
34.Gómez-Estaca J, Giménez B. Montero P, et al. Incorporation of antioxidant borage extract into edible films based on sole skin gelatin or a commercial fish gelatin[J]. Journal of Food Engineering, 2009, 92(1): 78-85
35.Gómez-Guillén M C, Ihl M, Bifani V, et al. Edible films made from tuna-fish gelatin with antioxidant extracts of two different murta ecotypes leaves (Ugni molinae Turcz)[J]. Food Hydrocolloids, 2007, 1: 1133-1143
36.Gómez-Estaca J, Bravo L, Gómez-Guillén M C. et al. Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extract[J]. Food Chemistry, 2009, 112: 18-25
37.Jongjareonrak A, Benjakul S, Visessanguanb W,et al. Antioxidative activity and properties of fish skin gelatin films incorporated with BHT and a-tocopherol[J]. Food Hydrocolloids, 2008, 22: 449-458
38.Muyonga J H, Cole C G B, Duodu K G. Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin[J]. Food Hydrocolloids, 2004, 18: 581-592
39.Kim S K, Kim Y T, Byun H G, et al. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska Pollack skin[J]. Journal of Agricultural and Food Chemistry, 2001, 49: 1984-1989
40.Mady M M. Biophysical studies on collagen-lipid interaction[J]. Journal of Bioscience and Bioengineering, 2007, 104(2): 144-148
41.Siebert K J, Troukhanova N V, Lynn P Y. Nature of polyphenol-protein interactions[J]. Journal of Agricultural and Food Chemistry, 1996, 44: 80-85
42.Fraziera R A, Deaville E R, Greenc R J, Interactions of tea tannins and condensed tannins with proteins[J]. Journal of Pharmaceutical and Biomedical Analysis, 2010, 51: 490-495
43.Papadopoulou A, Frazier R A. Characterization of protein-polyphenol interactions[J]. Trends in Food Science and Technology, 2004, 15: 186-190
44.Riedl, K M, Hagerman A E. Tannin-protein complexes as radical scavengers and radical sinks[J]. Journal of Agricultural and Food Chemistry, 2001, 49(10): 4917-4923
1.Karim A A, Bhat R. Gelatin alternatives for the food industry: Recent developments, challenges and prospects[J]. Trends in Food Science & Technology, 2008, 19: 644-656
2.曹娜,符玉华,贺军辉.明胶膜的性能研究进展[J].高分子通报, 2007, 8: 1-6
3.Yang L, Paulson A T. Effects of lipids on mechanical and moisture barrier properties of edible gellan film[J]. Food Research International, 2002, 33: 571-578
4.Bertan L C, Tanada-Palmua P S, Siani A C, et al. Effect of fatty acids and‘Brazilian elemi’on composite films based on gelatin[J]. Food Hydrocolloids, 2005, 19: 73-82
5. Sztuka K, Ko1odziejska I. The influence of hydrophobic substances on water vapor permeability of fish gelatin films modified with transglutaminase or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)[J]. Food Hydrocolloids, 2009, 23: 1062-1064
6.钟秋平,夏文水.壳聚糖木薯淀粉明胶复合可食抗菌保鲜膜性能的研究[J].食品科学, 2006, 27(6):59-64
7.Wu Q X, Zhang L N. Structure and properties of casting films blended with starch and waterborne polyurethane[J]. Journal of Applied Polymer Science, 2001, 79: 2006-2013
8.Xua Y X, Kimb K M, Hanna M A, et al. Chitosan-starch composite film: preparation and characterization[J]. Industrial Crops and Products, 2005, 21: 185-192
9.Harish Prashanth KV and Tharanathan RN, Chitin/chitosan: modifications and their unlimited application potential-an overview[J]. Trends in Food Science and Technology, 2007, 18: 117-131
10.Ravi Kumar M N V, Muzzarelli R A A, Muzzarelli C, et al. Chitosan Chemistry and Pharmaceutical Perspectives[J]. Chemical Reviews, 2004, 104(12): 6017-6084
11.王宇,陈大为,乔明曦,等.离子胶凝法制备水杨酸壳聚糖纳米粒[J].沈阳药科大学学报,2007,24(10):607-610
12.Vandenberg G W, Drolet C, Scott S L, et al. Factors affecting protein release from alginate-chitosan coacervate microcapsules during production and gastric/intestinal simulation[J]. Journal of Controlled Release, 2001, 77: 297-307
13.费轶博,李凤前,胡晋红,等.用离子交联-均化工艺制备乙肝疫苗壳聚糖纳米粒[J].药学服务与研究,2008,8(2):119-122
14.Sunil A A, Nadagouda N M, Tejraj M A. Recent advance on chitosan-based micro-and nanoparticles in drug delivery[J]. Journal of Control release, 2004, 100(1): 5-28
15.黄小龙,张黎明.壳聚糖基载药纳米微粒制备研究进展[J].功能高分子学报,2003,16:593-597
16.杨贤强,王岳飞,陈留记.茶多酚化学[M].上海:上海科学技术出版社,2003
17.Muèller R H, Maèder K, Gohla S. Solid lipid nanoparticles (SLN) for controlled drug delivery-a review of the state of the art [J]. European Journal of Pharmaceutics and Biopharmaceutics, 2000, 50(1): 161.
18.Heurtault B, Saulnier P, Pech B, et al. Physico-chemical stability of colloidal lipid particles[J]. Biomaterials, 2003,24: 4283-4300
19.Taravel M N, Domard A. Relation between the physicochemical characteristics of collagen and its interaction with chitosan: I[J]. Biomaterials, 1993, 14: 930-938
20.Taravel M N, Domard A. Collagen and its interactions with chitosan: II. Influence of the physicochemical characteristics of collagen[J]. Biomaterials, 1995, 16: 865-876
21.Taravel M N, Domard A. Collagen and its interactions with chitosan: III. Some biological and mechanical properties[J]. Biomaterials, 1996, 17: 451-455
23.Chen Z G, Mo X M, He C L, et al. Intermolecular interactions in electrospun collagen-chitosan complex nanofibers[J]. Carbohydrate Polymers, 2008, 72: 410-418
24.Sionkowska A, Wisniewski M, Skopinska J, et al. Molecular interactions in collagen and chitosan blends[J]. Biomaterials, 2004, 25: 795-801
25.Bigi A, Panzavolta S, Rubini K. Relationship between triple-helix content and mechanical properties of gelatin films[J]. Biomaterials, 2004 25: 5675-5680
26.Siebert K J, Troukhanova N V, Lynn P Y. Nature of polyphenol-protein interactions[J]. Journal of Agricultural and Food Chemistry, 1996, 44: 80-85
27.Papadopoulou A, Frazier R A. Characterization of protein-polyphenol interactions[J]. Trends in Food Science and Technology, 2004, 15: 186-190
28.Cao N, Fu YH, He JH, Mechanical properties of gelatin films cross-linked, respectively, by ferulic acid and tannin acid[J]. Food Hydrocolloid, 2007, 21: 575-584
29.蒋挺大.壳聚糖[M].北京:化学工业出版社, 2007
30.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
31.Arvanitoyannisa I S, Nakayama A, Aibab Sei-ichi. Chitosan and gelatin based edible films: state diagrams, mechanical and permeation properties[J]. Carbohydrate Polymers, 1998, 37: 371-382
32.Riedl, K M, Hagerman A E. Tannin-protein complexes as radical scavengers and radical sinks[J]. Journal of Agricultural and Food Chemistry, 2001, 49(10): 4917-4923
33.Gómez-Estaca J, Bravo L, Gómez-Guillén MC, et al. Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extract[J]. Food Chemistry, 2009, 112: 18-25
1.Desai M P, Labhasetwar V, Amidon G L, et al. Gastrointestinal Uptake of Biodegradable Microparticles: Effect of Particles. Pharmaceutical Research, 1996, 13(12): 1838-1845
2.张云龙,谢英,王会娟等.超氧化物歧化酶脂质体在大鼠体内的药代动力学和组织分布[J].药学学报,2005,40(2): 173-177
3.王瓞,宣海星,林其谁等.药物传递系统和传递策略[J].药物生物技术,1999,6(3): 181-187
4.黄秋婷,黄惠华.微胶囊技术在功能性油脂生产中的应用[J].中国油脂, 2005, 30(3): 27-29
5.李瑜,罗飞,许时婴.大蒜生物活性功能及蒜粉微胶囊化的研究进展[J].食品科学, 2006, 28(9):610-613
6.丁立忠,杨剑,黄文.食用香精微胶囊化研究进展[J].中国调味品, 2009, 34: 90-95
7.许时婴,张晓鸣,夏书芹,等.微胶囊技术-原理与应用[M].北京:化学工业出版社, 2006
8.Huang X, Brazel C S. On the importance and mechanisms of burst release in matrix-controlled drug delivery systems[J]. Journal of Controlled Release, 2001, 73: 121-136
9.Colombo P, Bettini R, Santi P, et al. Analysis of the swelling and release mechanisms from drug delivery systems with emphasis on drug solubility and water transport[J]. Journal of Controlled Release, 1996, 39: 231-237
10.Li Y Q, Wong H L, Shuhendler A J, et al. Molecular interactions, internal structure and drug release kinetics of rationally developed polymer-lipid hybrid nanoparticles[J]. Journal of Controlled Release, 2008, 128: 60-70
11.Liu Z, Wu X Y, Ballinger J R, et al. Synthesis and characterization of surface-hydrophobic ion-exchange microspheres and the effect of coating on drug release rate[J]. Journal of Pharmaceutical science, 2000,
89: 807-817
12.He P, Davis S S, Illum L. Chitosan microspheres prepared by spray drying[J]. International Journal of Pharmaceutics, 1999, 187: 53-65
13.Al-Helw A A, Al-Angary A A, Mahrous G M, et al. Preparation and evaluation of sustained release cross-linked chitosan microspheres containing phenobarbitone[J]. Journal of Microencapsulation, 1998, 15: 373-382
14.Sunil A A, Nadagouda N M, Tejraj M A. Recent advances on chitosan-based micro-and nanoparticles in drug delivery[J]. Journal of Control release, 2004, 100(1): 5-28
15.Pothakamury U R, Barbosa-Ganovas G V. Fundamental aspects of controlled release in foods[J]. Trends in Food Science and Technology, 1995, 6: 397-406
16.Yakimets I, Wellner N, Smith A C, et al. Mechanical properties with respect to water content of gelatin films in glassy state. Polymer, 2005, 46: 12577-12585
17.Chavanpatil M D, Jain P, Chaudhari S,et al. Novel sustained release, swellable and bioadhesive gastroretentive drug delivery system for ofloxacin[J]. International Journal of Pharmaceutics, 2006, 316:86-92
18.Gulsen D, Chauhan A. Effect of water content on transparency, swelling, lidocaine diffusion in p-HEMA gels[J]. Journal of Membrane Science, 2006, 269: 35-48
19.Lee P I, Kim C. Probing the mechanisms of drug release from hydrogels[J]. Journal of ControlledRelease, 1991, 16: 229-236
20.Shi X Y, Ma W Y, Sun C M, et al. The aggregation behavior of collagen in aqueous solution and its property of stabilizing liposomes in vitro[J]. Biomaterials, 2001, 22(12): 1627-1634
21.Mady M M. Biophysical studies on collagen-lipid interaction[J]. Journal of Bioscience and Bioengineering, 2007, 104(2): 144-148
22.Lin M, Meng S, Zhong W, et al. Novel drug-loaded gelatin films and their sustained-release performance[J]. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009, 90B: 939-944
23.Li W P A, Mhando J R. Formulation of sustained-release products: dissolution and diffusion-controlled release from gelatin films[J]. International Journal of Pharmaceutics, 1984, 20(1): 87-98
2. Ward A G, Courts A.明胶的科学与工艺学[M].李文渊等译,彭必先校.北京:中国轻工业出版社, 1982
3.王颖,陈丽娟,彭必先.鱼明胶[J].明胶科学与工艺学,1997,17(3): 113-123
4.Karim A A, Rajeev Bhat. Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins[J]. Food Hydrocolloids, 2009, 23: 563-576
5.Djagny K B, Wang Z, Xu S Y. Gelatin: a valuable protein for food and pharmaceutical industries: review[J]. Critical Reviews in Food Science and Nutrition, 2001, 41(6): 481-492
6.《明胶生产工艺及设备》编写组.明胶生产工艺及设备[M].北京:中国轻工业出版社, 1986
7.上海明胶厂.皮胶明胶的生产[M].上海:上海人民出版社, 1973
8.苏州化工厂.骨胶明胶的生产[M].上海:上海人民出版社, 1977
9.任乃容.我国明胶科研十年简要回顾(二)(1981～1990)[J].明胶科学与技术, 1995, 15(3): 9-14
10.徐润,梁庆华.明胶的生产及应用技术[M].北京:中国轻工业出版社, 1997
11.Zhou P, Regenstein J M. Optimization of extraction conditions for Pollock skin gelatin[J]. Journal of Food Science, 2004, 69(5): C392-C398
12.Ladislaus M K, Yan X, Yao W L, et al. Optimization of gelatine extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology[J]. Bioresource Technology, 2007, 98: 3338-3343
13.Gime′neza B, Turnayb J, Lizarbeb M A, et al. Use of lactic acid for extraction of fish skin gelatin[J]. Food Hydrocolloids, 2005, 19: 941-950
14.刘小玲,许时婴.从鸡骨中制取明胶的加工工艺[J].食品与发酵工业,2004,30(10): 48-53
15.刘海英.斑点差尾鮰鱼(channel catfish)皮胶原蛋白及胶原多肽的研究[D]:[博士学位论文].江苏:江南大学, 2007
16.刘丽娜.鮰鱼皮明胶的制备及其功能性质研究[D]:[硕士学位论文].江苏:江南大学, 2008
17.Cho S M, Kwak K S, Park D C, et al. Processing optimization and functional properties of gelatin from shark (Isurus oxyrinchus) cartilage [J]. Food Hydrocolloids, 2004, 18: 573-579
18.张峰.酶法制备明胶的研究[D]:[硕士研究生学位论文].北京:北京化工大学, 2002
19.陈秀金,侯颖,张敏,等. 2709碱性蛋白酶水解脱铬革屑的研究[J].中国皮革, 2007, 36(19): 47-50
20.侯国泉,王璋.酶法从猪皮制备食用明胶工艺的研究[J].无锡轻工业学院学报, 1992, 11(1): 10-20
21.韩应昌,许时婴.酶法制取骨照相明胶工艺的研究[J].无锡轻工业学院学报, 1993, 12(1): 1-13
22.安广杰,徐清萍. Neutrase蛋白酶降解骨胶原蛋白制备水解明胶[J].食品与机械, 2007, 23(3): 39-40
23.魏立新,杜玉枝,王安柱,等.碱性蛋白酶降解骨胶原制备食品明胶工艺[J].安徽农业科学, 2007, 35(20): 6235-6239
24.安广杰,王璋,许时婴.利用Alcalase蛋白酶生产牛骨水解明胶[J].食品与发酵工业,2004, 30(3): 52-55
25.Nalinanon S, Benjakul, Visessanguan W, et al. Improvement of gelatin extraction from bigeye snapper skin using pepsin-aided process in combination with protease inhibitor [J]. Food Hydrocolloids, 2008, 22: 615-622
26.陈丽娟,王颖,彭必先.胰蛋白酶降解骨胶原与明胶组份含量之间的关系[J].感光科学与光化学,1998, 16(3): 258-263
27.Taylor M M, Cabeza L F, Marmer W N, et al. Use of tryptic enzyme preparations in treatment of chrome shavings [J]. Journal of the American Leather Chemists Association, 2000, 95(7): 243
28.吕坪,杜玉枝,魏立新.酶法制备明胶的研究进展[J].明胶科学与技术, 2006, 26(4): 170-176
29.Gómez-Guillén M C, Pérez-Mateos J, Gómez-Estaca E, et al. Fish gelatin: a renewable material for developing active biodegradable films[J]. Trends in Food Science & Technology, 2009, 20: 3-16
30.Gómez-Guillén M C, Turnay J, Fernandez-Diaz M D, et al. Structural and physical properties of gelatin extracted from different marine species: a comparative study[J]. Food Hydrocolloids, 2002, 16: 25-34
31.Leuenberger B H. Investigation of viscosity and gelatin properties of different mammalian and fish gelatins[J]. Food Hydrocolloids, 1991, 5: 353-356
32.Eastoe J E, Leach A A. Chemical constitution of gelatin[M]. London: Academic Press, 1977
33.史京京,陈丽娟,王颖,等.胶冻强度与明胶α组份关系的研究[J].感光科学与光化学, 2002, 20(6): 462-467
34.Young S, Wong M, Tabata Y, et al. Gelatin as a delivery vehicle for the controlled release of bioactive molecules[J]. Journal of Controlled Release, 2005, 109: 256-274
35.Karim A A, Bhat R. Gelatin alternatives for the food industry: Recent developments, challenges and prospects[J]. Trends in Food Science & Technology, 2008, 19: 644-656
36.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
37.Tharanathan RN. Biodegradable films and composite coatings: Past, present and future[J]. Trends in Food Science and Technology, 2003, 14: 71-78
38.曹娜,符玉华,贺军辉.明胶膜的性能研究进展[J].高分子通报, 2007, 8: 1-6
39.Deng C M, He L Z, Zhao M, et al. Biological properties of the chitosan-gelatin sponge wound dressing[J]. Carbohydrate Polymers, 2007, 69: 583-589
40.黄志,许时婴.利用猪胶皮制作可食性胶元人造肠衣的研究[J].食品与发酵工业, 1990, 5: 9-17
41.Gennadios A, Hanna M A, Kurth LB. Application of edible coating on meats, poultry and seafoods: A review. LWT, 1997, 30: 337-350
42.Bigi A, Panzavolta S, Rubini K. Relationship between triple-helix content and mechanical properties of gelatin films[J]. Biomaterials, 2004, 25: 5675-5680
43.刘小杰,齐鲁,白立峰.改性明胶膜的制备及其力学性能探讨[J].天津工业大学学报, 2006, 25(2): 19-21, 32
44.Cao N, Yang X M, Fu Y. Effects of various plasticizers on mechanical and water vapor barrier properties of gelatin films[J]. Food Hydrocolloids, 2009, 23: 729-735
45.Bigi A, Cojazzi G, Panzavolta S, et al. Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking[J]. Biomaterials, 2001, 22: 763-768
46.Sazedul Hoque M, Benjakul S, Prodpran T. Effect of heat treatment of film-forming solution on the properties of film from cuttlefish (Sepia pharaonis) skin gelatin[J]. Journal of Food Engineering, 2010, 96: 66-73
47.Chambi H, Grosso C. Edible films produced with gelatin and casein cross-linked with transglutaminase[J]. Food Research International, 2006, 39: 458-466
48.Cao N, Fu Y H, He J H. Mechanical properties of gelatin films cross-linked, respectively, by ferulic acid and tannin acid[J]. Food Hydrocolloids, 2007, 21: 575-584
49.Pranotoa Y, Lee C M, Park H J. Characterizations of fish gelatin films added with gellan andκ-carrageenanL[J]. LWT, 2007, 40: 766-774
50.Bertan L C, Tanada-Palmu P S, Siani A C, et al. Effect of fatty acids and“Brazilian elemi”on composite films based on gelatin[J]. Food Hydrocolloids, 2005, 19: 73-82
51.Cao N, Fu Y H, He J H. Preparation and physical properties of soy protein isolate and gelatin composite films[J]. Food Hydrocolloids, 2007, 21: 1153-1162
52.余家会,杜予民,郑化.壳聚糖明胶共混膜[J].武汉大学学报(自然科学版), 1999, 45(4): 440-444
53.李贵安,苗润才.纳米银明胶复合膜的制作[J].陕西师范大学学报(自然科学版), 1999, 27(2): 37-39
54.Lopez-Rubio A, Gavara R, Lagaron J M. Bioactive packaging: turning foods into healthier foods through biomaterials[J]. Trends in Food Science & Technology, 2006, 17: 567-575
55.Gómez-Estaca J, Bravo L, Gómez-Guillén MC, et al. Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extract[J]. Food Chemistry, 2009, 112: 18-25
56.Gime′nez B, Go′mez-Estaca J, Alema′n A, et al. Improvement of the antioxidant properties of squid skin gelatin films by the addition of hydrolysates from squid gelatin[J]. Food Hydrocolloids, 2009, 23: 1322-1327
57.Jongjareonrak A, Benjakul S, Visessanguanb W, et al. Antioxidative activity and properties of fish skin gelatin films incorporated with BHT andα-tocopherol. Food Hydrocolloids, 2008, 22: 449-458
58.赵铁,杜予民,唐汝培.壳聚糖水杨酸盐明胶共混膜结构表征及其抗菌性[J].分析科学学报, 2002, 18(2): 100-104
59.白立峰,齐鲁,刘小杰.含有奥硝哇纳米囊泡的改性明胶膜释药性能的研究[J].中国新药杂志, 2006, 15(24): 2132-2136
60.高玉香,阎玉华,金中秋.眼用药膜的研制及其性能表征[J].武汉理工大学学报, 2009, 31(16): 54-57
61.张团红,胡小玲,乔吉超,等.纳米胶囊的制备与应用进展[J].材料科学与工程学报, 2007, 25(1): 143-146
62.Letchford K, Burt H. A review of the formation and classification of amphiphilic block copolymer nanoparticulate structures: micelles, nanospheres, nanocapsules and polymersomes[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2007, 65: 259-269
63.Zimmer A, Kreuter J?rg. Microspheres and nanoparticles used in ocular delivery systems[J]. Advanced Drug Delivery Reviews, 1995, 16(1): 61-73
64.Sozer N, Kokini J L. Nanotechnology and its applications in the food sector[J]. Trends in Biotechnology, 2009, 27(2): 82-89
65.Mozafari M R. Commentary: Amphiphiles and their aggregates in basic and applied science. A post-conference thought on nomenclature [J]. Cellular & Molecular Biology Letters, 2005, 10(4): 733–734
66.夏书芹.辅酶Q10纳米脂质体的研究[D]:[博士学位论文].江苏:江南大学, 2007
67.许时婴,张晓鸣,夏书芹,等.微胶囊技术─原理与应用[M].北京:化学工业出版社, 2006
68.曹宁宁,羡菲.脂质体的制备方法及研究进展[J].天津理工学院学报, 2003, 19(1): 30-35
69.Patel V B, Misra A N. Encapsulation and stability of clofazimine liposomes[J]. Journal of Microencapsulation, 1999, 16: 357-367
70.Pons M, Foradada M, Estelrich J. Liposomes obtained by the ethanol injection method[J]. International Journal of Pharmaceutics, 1993, 95: 51-56
71.Xia S Q, Xu S Y, Zhang X M. Optimization in the preparation of coenzyme Q10 nanoliposomes[J]. Journal of Agricultural and Food Chemistry, 2006, 54(17): 6358-6366
72.Bangham A D, Standish M M, Watkins J C. Diffusion of univalent ions across the lamellae of swollen phospholipids[J]. Journal of Molecular Biology, 1965, 13: 238-252
73.Maestrelli F, Gonzalez-Rodriguez M L, Rabasco A M, et al. Effect of preparation technique on theproperties of liposomes encapsulating ketoprofen-cyclodextrin complexes aimed for transdermal delivery[J]. International Journal of Pharmaceutics, 2006, 298: 53-60
74.Lasic D D. Liposomes: from physics to application[M]. Netherlands: Elsevier, 1993
75.Vyas S P, Singh R P, Jain S, et al. Non-ionic surfactant based vesicles (niosomes) for non-invasive topical genetic immunization against hepatitis B[J]. International Journal of Pharmaceutics, 2005, 296: 80-86
76.邓意辉,王鹤东.主动载药与被动载药制备盐酸左氧氟沙星脂质体[J].中国抗生素杂志,2001,26(4):258-260
77.蒋挺大.壳聚糖[M].北京:化学工业出版社, 2007
78.Kosaraju S L, D’ath L, Lawrence A. Preparation and characterisation of chitosan microspheres for antioxidant delivery[J]. Carbohydrate Polymers, 2006, 64: 163-167
79.Harish Prashanth KV and Tharanathan RN, Chitin/chitosan: modifications and their unlimited application potential-an overview[J]. Trends in Food Science and Technology, 2007, 18: 117-131
80.张玮,张学农.壳聚糖纳米粒制备技术研究进展[J].抗感染药学, 2008, 5(2): 65-69
81.Agnihotri S A, Mallikarjuna N N, Aminabhavi T M. Recent advances on chitosan-based micro- and nanoparticles in drug delivery[M]. Journal of Controlled Release, 2004, 100: 5-28
82.Bergera J, Reista M, Mayer J M, et al. Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2004, 57: 19-34
83.徐忻.壳聚糖纳米载体研究[D]:[博士学位论文].吉林:东北师范大学, 2004
84.Zhang S, Wang Y, Herring J L, et al. Characterization of edible film fabricated with channel catfish (Ictalurus punctatus) gelatin extract using selected pretreatment methods[J]. Journal of Food Science, 2007, 72(9): 498-503
85.Li Y Q, Wong H L, Shuhendler A J, et al. Molecular interactions, internal structure and drug release kinetics of rationally developed polymer-lipid hybrid nanoparticles[J]. Journal of Controlled Release, 2008, 128: 60-70
86.Sunil A A, Nadagouda N M, Tejraj M A. Recent advances on chitosan-based micro-and nanoparticles in drug delivery[J]. Journal of Control release, 2004, 100(1): 5-28
87. Pothakamury U R, Barbosa-Ganovas G V. Fundamental aspects of controlled release in foods[J]. Trends in Food Science and Technology, 1995, 6: 397-406
88.Lin M, Meng S, Zhong W, et al. Novel drug-loaded gelatin films and their sustained-release performance[J]. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009, 90B: 939-944
89.Li W P A, Mhando J R. Formulation of sustained-release products: dissolution and diffusion-controlled release from gelatin films[J]. International Journal of Pharmaceutics, 1984, 20(1): 87-98
90.杨贤强,王岳飞,陈留记.茶多酚化学[M].上海:上海科学技术出版社, 2003
91.Friedman M. Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas[J]. Molecular Nutrition & Food Research, 2007, 51: 116-134
92.孟祥,李保国,齐正.茶多酚及其微胶囊化研究进展[J].食品科技, 2006, 11: 37-39
1.蒋挺大.胶原与胶原蛋白[M].北京:化学工业出版社, 2006
2.Vuorio E, Crombrugghe B. The family of collagen genes[J].Annu Review Biochemistry, 1990, 50: 872-873
3.Ward A G, Courts A.明胶的科学与工艺学[M].李文渊等译.北京:中国轻工业出版社, 1982
4.Djagny K B, Wang Z, Xu S Y. Gelatin: a valuable protein for food and pharmaceutical industries: review[J]. Critical Reviews in Food Science and Nutrition, 2001, 41(6): 481-492
5.Karim A A, Rajeev Bhat. Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins[J]. Food Hydrocolloids, 2009, 23: 563-576
6.谢音,屈小英.食品分析[M].北京:科学技术文献出版社, 2006
7.殷明文,南宇梅,王新民.分光光度法测定羟脯氨酸的改进[J].河南医科大学学报, 1994, 29(1): 74-77
8.刘海英.斑点差尾鮰鱼(channel catfish)皮胶原蛋白及胶原多肽的研究[D]:[博士学位论文].江苏:江南大学, 2007
9.侯国泉.酶法从猪皮制备食用明胶工艺的研究[D]:[硕士学位论文].江苏:无锡轻工业大学, 1992
10.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
11.Skierka E, Sadowska M. The influence of different acids and pepsin on the extractability of collagen from the skin of Baltic cod(Gadus morhua)[J]. Food Chemistry, 2007, 105: 1302–1306
12.Liu H Y, Li D, Guo S D. Studies on collagen from the skin of channel catfish(Ictalurus Punctaus)[J]. Food Chemistry, 2007, 101(2): 621-625
13.Zeng S K, Zhang C H, Lin H, et al. Isolation and characterisation of acid-solubilised collagen from the skin of Nile tilapia (Oreochromis niloticus)[J]. Food Chemistry, 2009, 116: 879-883
14.胡胜,李志强,陈敏.皮胶原蛋白的酶法提取及在高附加值领域的应用[J].皮革科学与工程, 2002, 12(5): 38-43
15.Li Y, Li Y W, Du Z L, et al. Comparison of dynamic denaturation temperature of collagen with its static denaturation temperature and the configuration characteristics in collagen denaturation processes[J]. Thermochimica Acta, 2008, 469: 71-76
16.刘小玲,许时婴.从鸡骨中制取明胶的加工工艺[J].食品与发酵工业, 2004, 30(5): 48-53
17.Ko?odziejska I, Skierka E, Sadowska M, et al. Effect of extracting time and temperature on yield of gelatin from different fish offal[J]. Food Chemistry, 2008, 107: 700-706
18.Zeugolis D I, Paul R G, Attenburrow G. Post-self-assembly experimentation on extruded collagen fibres for tissue engineering applications[J]. Acta Biomaterialia, 2008, 4: 1646-1656
19.刘小玲.鸡骨明胶的制备、结构及功能性质研究[D]:[博士学位论文].江苏:江南大学, 2005
20.Usha R, Ramsaami T. Structure and conformation of intramolecularly cross-linked collagen[J]. Colloids and Surfaces B, 2005, 41: 21-24.
21.Muyonga J H, Cole C G B, Duodu K G. Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus)[J]. Food Chemistry, 2004, 85: 81-89
22.Nagai T, Araki Y, Suzuki N. Collagen of the skin of ocellate puffer fish (Takifugu rubripes)[J]. Food Chemistry, 2002, 78: 173-177
23.Burjandze, T. V. Hydroxy-proline content and location in relation to collagen thermal stability. Biopolymers, 1979, 18: 931-936
24.Barbara B, John A M R. The collagen triple-helix Structure[J]. Matrix Biology, 1997, 15(8-9): 545-554
25.Piez K A, Gross J. The amino acid composition of some fish collagens: The relation between composition and structure[J]. Journal of Biological Chemistry, 1960, 235: 995-998
26.John A M R, Jerome W, Allan Bremner. Characterization of type I collagen from skin of Blue Grenadier[J]. Archives of Biochemistry and Biophysics, 1988, 267(2): 497-502
27.阎隆飞,孙之荣.蛋白质分子结构[M].北京:清华大学出版社, 1999
28.刘丽娜.鮰鱼皮明胶的制备及其功能性质研究[D]:[硕士学位论文].江苏:江南大学, 2008
29.Fathima N N, Bose M C, Rao J R, et al. Stabilization of type I collagen against collagenases (type I) and thermal degradation using iron complex[J]. Journal of Inorganic Biochemistry, 2006, 100: 1774-1780
30.Ikoma T, Kobayashi H, Tanaka J, et al. Physical properties of type I collagen extracted from fish scales of Pagrus major and Oreochromis niloticas[J]. International Journal of Biological Macromolecules, 2003, 32: 199-204
31.熊良文,彭必先.圆二色光谱法研究某些助剂对明胶大分子的多极结构的影响[J].感光科学与光化学, 1989, 4: 1-5
32.Gómez-Guillén M C, Turnay J, Fernandez-Diaz M D, et al. Structural and physical properties of gelatin extracted from different marine species: a comparative study[J]. Food Hydrocolloids, 2002, 16: 25-34
33.张俐娜,薛奇,莫志深,等.高分子物理现代研究方法[M].武汉:武汉大学出版社, 1989
34.Gilsenan P M, Ross-Murphy S B. Rheological characterization of gelatins from mammalian and marine sources[J]. Food Hydrocolloids, 2000, 14: 191-19
1.吴韶敏,曹劲松.脂质体技术应用于食品工业的最新研究进展[J].中国油脂,2007,(32):42-46
2.曹宁宁,羡菲.脂质体的制备方法及研究进展[J].天津理工学院学报, 2003, 19(1): 30-35
3.夏书芹.辅酶Q10纳米脂质体的研究[D]:[博士学位论文].江苏:江南大学, 2007
4.范明辉.红景天苷纳米脂质体的研制[D]:[博士学位论文].江苏:江南大学, 2008
5.刘小玲.鸡骨明胶的制备、结构及功能性质研究[D]:[博士学位论文].江苏:江南大学, 2005
6.殷小梅,许时婴.可食茁霉多糖膜的结构与性质研究[J].食品科学, 1998, 19(2): 3-6
7.傅樱花,马长伟.腊肉加工过程中脂质分解及氧化的研究[J].食品科技, 2004, 1: 42-45
8. Fan M H, Xu S Y, Xia S Q, et al. Preparation of salidroside nano-liposomes by Ethanol injection method and in vitro release study[J]. European Food and Research Technology, 2008, 227:167-174
9.Nakayama T, Hashimoto T, Kajiya K, et al. Affinity of polyphenols for lipid bilayers[J]. BioFactors, 2000, 13: 147-151
10.Tanaka T, Kusano R, Kouno I. Synthesis and antioxidant activity of novel amphipathic derivatives of tea polyphenol[J]. Bioorganic & Medicinal Chemistry Letters, 1998, 8: 1801-1806
11.Fang J Y, Lee W R , Shen S C, et al. Effect of liposome encapsulation of tea catechins on their accumulation in basal cell carcinomas[J]. Journal of Dermatological Science, 2006, 42: 101-109
12.Imura T, Otake K, Hashimoto S, et al. Preparation and physicochemical properties of various soybean lecithin liposomes using supercritical reverse phase evaporation method[J]. Colloids and Surfaces B, 2002, 27: 133-140
13.Patel V B, Misra A N. Encapsulation and stability of clofazimine liposomes[J]. Journal of microencapsulation, 1999, 16: 357-367
14.Plessis J, Ramchandran C, Weiner N, et al. The influence of lipid composition and lamellarity of liposomes on the physical stability of liposomes upon storage[J]. International Journal of Pharmaceutics, 1996, 127: 273-278
15.Agarwal R, Katare O P, Vyas S P. Preparation and in vitro evaluation of liposomal/niosomal delivery systems for antipsoriatic drug dithranol[J]. International Journal of Pharmaceutics, 2001, 228: 43-52
16.Jemiota-Rzeminska M, Kruk J, Skowronek M, et al. Location of ubiquinone homologues in liposome membranes studied by fluorescence anisotropy of diphenylhexatriene and trimethylammonium-diphenyl-hexatriene[J]. Chemistry and Physics of Lipids, 1996, 79(1): 55-63
17.Tasi L M, Liu D Z, Chen W Y. Microcalorimetric investigation of the interaction of polysorbate surfactants with unilamellar phosphatidylcholines liposomes[J]. Colloids and Surfaces A 2003, 213: 7-14
18.Takeuchi H, Kojima H, Yamamoto H, et al. Polymer coating of liposomes with a modified polyvinyl alcohol and their systemic circulation and RES uptake in rats[J]. Journal of Controlled Release, 2000, 68: 195-205
19.Carrion C, Domingo J C, Madariaga M A. Preparation of long-circulating immuno-liposomes using PEG-cholesterol conjugates: effect of the spacer arm between PEG and cholesterol on liposomal characteristics[J]. Chemistry and Physics of Lipids, 2001, 113: 97-110
20.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
21.Crosas E, Egea M A, Reig F. Spectroscopic techniques applied to the study of laminin fragments inserted into model membranes[J]. Journal of Colloid and Interface Science, 2006, 295(1): 264-269
22.Biasio A D, Bordi F, Cametti C. Salt-induced aggregation in cationic liposome suspensions[J]. Progress in Colloid and Polymer Science, 2004, 123: 78-82
23.夏书芹,许时婴.辅酶Q10纳米脂质体的制备[J].食品工业科技,2006,2:164-168
24.Schwarz C, Mehnert W, Lucks JS, et al. Solid lipid nanoparticles (SLN) for controlled drug delivery. I.Production, characterization and sterilization[J]. Journal of Controlled Release, 1994, 30: 83-96
25.Silvander M. Steric stabilization of liposomes- a review[J]. Progress in Colloid and Polymer Science, 2002, 120: 35-40
26.Gontard N, Duchez C, Cuq J L, et al. Edible composite films of wheat and lipids: Water vapour permeability and other physical properties[J]. International Journal of Food Science and Technology, 1994, 29: 39-50
27.Bertan L C, Tanada-Palmua P S, Siani A C, et al. Effect of fatty acids and“Brazilian elemi”on composite films based on gelatin[J]. Food Hydrocolloids, 2005, 19: 73-82
28.Jiang M K, Liu S Y, Du X, Physical properties and internal microstructures of films made from catfish skin gelatin and triacetin mixtures[J]. Food Hydrocolloids, 2010, 24: 105-110
29.Yakimets I, Wellner N, Smith A C, et al. Mechanical properties with respect to water content of gelatin films in glassy state. Polymer, 2005, 46: 12577-12585
30.Shi X Y, Ma W Y, Sun C M, et al. The aggregation behavior of collagen in aqueous solution and its property of stabilizing liposomes in vitro[J]. Biomaterials, 2001, 22: 1627-1634
31.杨贤强,王岳飞,陈留记.茶多酚化学[M].上海:上海科学技术出版社, 2003
32.Dwiecki K, Górnas P, Wilk A, et al. Spectroscopic studies of D-α-tocopherol concentration-induced transformation in egg phosphatidylcholne vesicles[J]. Cellular Molecular Biology Letters, 2007, 12(1): 51-69
33.谢晶曦,常俊标.红外光谱-在有机化学和药物化学中的应用[M].北京:科学出版社,2001
34.Gómez-Estaca J, Giménez B. Montero P, et al. Incorporation of antioxidant borage extract into edible films based on sole skin gelatin or a commercial fish gelatin[J]. Journal of Food Engineering, 2009, 92(1): 78-85
35.Gómez-Guillén M C, Ihl M, Bifani V, et al. Edible films made from tuna-fish gelatin with antioxidant extracts of two different murta ecotypes leaves (Ugni molinae Turcz)[J]. Food Hydrocolloids, 2007, 1: 1133-1143
36.Gómez-Estaca J, Bravo L, Gómez-Guillén M C. et al. Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extract[J]. Food Chemistry, 2009, 112: 18-25
37.Jongjareonrak A, Benjakul S, Visessanguanb W,et al. Antioxidative activity and properties of fish skin gelatin films incorporated with BHT and a-tocopherol[J]. Food Hydrocolloids, 2008, 22: 449-458
38.Muyonga J H, Cole C G B, Duodu K G. Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin[J]. Food Hydrocolloids, 2004, 18: 581-592
39.Kim S K, Kim Y T, Byun H G, et al. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska Pollack skin[J]. Journal of Agricultural and Food Chemistry, 2001, 49: 1984-1989
40.Mady M M. Biophysical studies on collagen-lipid interaction[J]. Journal of Bioscience and Bioengineering, 2007, 104(2): 144-148
41.Siebert K J, Troukhanova N V, Lynn P Y. Nature of polyphenol-protein interactions[J]. Journal of Agricultural and Food Chemistry, 1996, 44: 80-85
42.Fraziera R A, Deaville E R, Greenc R J, Interactions of tea tannins and condensed tannins with proteins[J]. Journal of Pharmaceutical and Biomedical Analysis, 2010, 51: 490-495
43.Papadopoulou A, Frazier R A. Characterization of protein-polyphenol interactions[J]. Trends in Food Science and Technology, 2004, 15: 186-190
44.Riedl, K M, Hagerman A E. Tannin-protein complexes as radical scavengers and radical sinks[J]. Journal of Agricultural and Food Chemistry, 2001, 49(10): 4917-4923
1.Karim A A, Bhat R. Gelatin alternatives for the food industry: Recent developments, challenges and prospects[J]. Trends in Food Science & Technology, 2008, 19: 644-656
2.曹娜,符玉华,贺军辉.明胶膜的性能研究进展[J].高分子通报, 2007, 8: 1-6
3.Yang L, Paulson A T. Effects of lipids on mechanical and moisture barrier properties of edible gellan film[J]. Food Research International, 2002, 33: 571-578
4.Bertan L C, Tanada-Palmua P S, Siani A C, et al. Effect of fatty acids and‘Brazilian elemi’on composite films based on gelatin[J]. Food Hydrocolloids, 2005, 19: 73-82
5. Sztuka K, Ko1odziejska I. The influence of hydrophobic substances on water vapor permeability of fish gelatin films modified with transglutaminase or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)[J]. Food Hydrocolloids, 2009, 23: 1062-1064
6.钟秋平,夏文水.壳聚糖木薯淀粉明胶复合可食抗菌保鲜膜性能的研究[J].食品科学, 2006, 27(6):59-64
7.Wu Q X, Zhang L N. Structure and properties of casting films blended with starch and waterborne polyurethane[J]. Journal of Applied Polymer Science, 2001, 79: 2006-2013
8.Xua Y X, Kimb K M, Hanna M A, et al. Chitosan-starch composite film: preparation and characterization[J]. Industrial Crops and Products, 2005, 21: 185-192
9.Harish Prashanth KV and Tharanathan RN, Chitin/chitosan: modifications and their unlimited application potential-an overview[J]. Trends in Food Science and Technology, 2007, 18: 117-131
10.Ravi Kumar M N V, Muzzarelli R A A, Muzzarelli C, et al. Chitosan Chemistry and Pharmaceutical Perspectives[J]. Chemical Reviews, 2004, 104(12): 6017-6084
11.王宇,陈大为,乔明曦,等.离子胶凝法制备水杨酸壳聚糖纳米粒[J].沈阳药科大学学报,2007,24(10):607-610
12.Vandenberg G W, Drolet C, Scott S L, et al. Factors affecting protein release from alginate-chitosan coacervate microcapsules during production and gastric/intestinal simulation[J]. Journal of Controlled Release, 2001, 77: 297-307
13.费轶博,李凤前,胡晋红,等.用离子交联-均化工艺制备乙肝疫苗壳聚糖纳米粒[J].药学服务与研究,2008,8(2):119-122
14.Sunil A A, Nadagouda N M, Tejraj M A. Recent advance on chitosan-based micro-and nanoparticles in drug delivery[J]. Journal of Control release, 2004, 100(1): 5-28
15.黄小龙,张黎明.壳聚糖基载药纳米微粒制备研究进展[J].功能高分子学报,2003,16:593-597
16.杨贤强,王岳飞,陈留记.茶多酚化学[M].上海:上海科学技术出版社,2003
17.Muèller R H, Maèder K, Gohla S. Solid lipid nanoparticles (SLN) for controlled drug delivery-a review of the state of the art [J]. European Journal of Pharmaceutics and Biopharmaceutics, 2000, 50(1): 161.
18.Heurtault B, Saulnier P, Pech B, et al. Physico-chemical stability of colloidal lipid particles[J]. Biomaterials, 2003,24: 4283-4300
19.Taravel M N, Domard A. Relation between the physicochemical characteristics of collagen and its interaction with chitosan: I[J]. Biomaterials, 1993, 14: 930-938
20.Taravel M N, Domard A. Collagen and its interactions with chitosan: II. Influence of the physicochemical characteristics of collagen[J]. Biomaterials, 1995, 16: 865-876
21.Taravel M N, Domard A. Collagen and its interactions with chitosan: III. Some biological and mechanical properties[J]. Biomaterials, 1996, 17: 451-455
23.Chen Z G, Mo X M, He C L, et al. Intermolecular interactions in electrospun collagen-chitosan complex nanofibers[J]. Carbohydrate Polymers, 2008, 72: 410-418
24.Sionkowska A, Wisniewski M, Skopinska J, et al. Molecular interactions in collagen and chitosan blends[J]. Biomaterials, 2004, 25: 795-801
25.Bigi A, Panzavolta S, Rubini K. Relationship between triple-helix content and mechanical properties of gelatin films[J]. Biomaterials, 2004 25: 5675-5680
26.Siebert K J, Troukhanova N V, Lynn P Y. Nature of polyphenol-protein interactions[J]. Journal of Agricultural and Food Chemistry, 1996, 44: 80-85
27.Papadopoulou A, Frazier R A. Characterization of protein-polyphenol interactions[J]. Trends in Food Science and Technology, 2004, 15: 186-190
28.Cao N, Fu YH, He JH, Mechanical properties of gelatin films cross-linked, respectively, by ferulic acid and tannin acid[J]. Food Hydrocolloid, 2007, 21: 575-584
29.蒋挺大.壳聚糖[M].北京:化学工业出版社, 2007
30.Fennema O R.食品化学[M].王璋等译.北京:中国轻工业出版社, 2003
31.Arvanitoyannisa I S, Nakayama A, Aibab Sei-ichi. Chitosan and gelatin based edible films: state diagrams, mechanical and permeation properties[J]. Carbohydrate Polymers, 1998, 37: 371-382
32.Riedl, K M, Hagerman A E. Tannin-protein complexes as radical scavengers and radical sinks[J]. Journal of Agricultural and Food Chemistry, 2001, 49(10): 4917-4923
33.Gómez-Estaca J, Bravo L, Gómez-Guillén MC, et al. Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extract[J]. Food Chemistry, 2009, 112: 18-25
1.Desai M P, Labhasetwar V, Amidon G L, et al. Gastrointestinal Uptake of Biodegradable Microparticles: Effect of Particles. Pharmaceutical Research, 1996, 13(12): 1838-1845
2.张云龙,谢英,王会娟等.超氧化物歧化酶脂质体在大鼠体内的药代动力学和组织分布[J].药学学报,2005,40(2): 173-177
3.王瓞,宣海星,林其谁等.药物传递系统和传递策略[J].药物生物技术,1999,6(3): 181-187
4.黄秋婷,黄惠华.微胶囊技术在功能性油脂生产中的应用[J].中国油脂, 2005, 30(3): 27-29
5.李瑜,罗飞,许时婴.大蒜生物活性功能及蒜粉微胶囊化的研究进展[J].食品科学, 2006, 28(9):610-613
6.丁立忠,杨剑,黄文.食用香精微胶囊化研究进展[J].中国调味品, 2009, 34: 90-95
7.许时婴,张晓鸣,夏书芹,等.微胶囊技术-原理与应用[M].北京:化学工业出版社, 2006
8.Huang X, Brazel C S. On the importance and mechanisms of burst release in matrix-controlled drug delivery systems[J]. Journal of Controlled Release, 2001, 73: 121-136
9.Colombo P, Bettini R, Santi P, et al. Analysis of the swelling and release mechanisms from drug delivery systems with emphasis on drug solubility and water transport[J]. Journal of Controlled Release, 1996, 39: 231-237
10.Li Y Q, Wong H L, Shuhendler A J, et al. Molecular interactions, internal structure and drug release kinetics of rationally developed polymer-lipid hybrid nanoparticles[J]. Journal of Controlled Release, 2008, 128: 60-70
11.Liu Z, Wu X Y, Ballinger J R, et al. Synthesis and characterization of surface-hydrophobic ion-exchange microspheres and the effect of coating on drug release rate[J]. Journal of Pharmaceutical science, 2000,
89: 807-817
12.He P, Davis S S, Illum L. Chitosan microspheres prepared by spray drying[J]. International Journal of Pharmaceutics, 1999, 187: 53-65
13.Al-Helw A A, Al-Angary A A, Mahrous G M, et al. Preparation and evaluation of sustained release cross-linked chitosan microspheres containing phenobarbitone[J]. Journal of Microencapsulation, 1998, 15: 373-382
14.Sunil A A, Nadagouda N M, Tejraj M A. Recent advances on chitosan-based micro-and nanoparticles in drug delivery[J]. Journal of Control release, 2004, 100(1): 5-28
15.Pothakamury U R, Barbosa-Ganovas G V. Fundamental aspects of controlled release in foods[J]. Trends in Food Science and Technology, 1995, 6: 397-406
16.Yakimets I, Wellner N, Smith A C, et al. Mechanical properties with respect to water content of gelatin films in glassy state. Polymer, 2005, 46: 12577-12585
17.Chavanpatil M D, Jain P, Chaudhari S,et al. Novel sustained release, swellable and bioadhesive gastroretentive drug delivery system for ofloxacin[J]. International Journal of Pharmaceutics, 2006, 316:86-92
18.Gulsen D, Chauhan A. Effect of water content on transparency, swelling, lidocaine diffusion in p-HEMA gels[J]. Journal of Membrane Science, 2006, 269: 35-48
19.Lee P I, Kim C. Probing the mechanisms of drug release from hydrogels[J]. Journal of ControlledRelease, 1991, 16: 229-236
20.Shi X Y, Ma W Y, Sun C M, et al. The aggregation behavior of collagen in aqueous solution and its property of stabilizing liposomes in vitro[J]. Biomaterials, 2001, 22(12): 1627-1634
21.Mady M M. Biophysical studies on collagen-lipid interaction[J]. Journal of Bioscience and Bioengineering, 2007, 104(2): 144-148
22.Lin M, Meng S, Zhong W, et al. Novel drug-loaded gelatin films and their sustained-release performance[J]. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009, 90B: 939-944
23.Li W P A, Mhando J R. Formulation of sustained-release products: dissolution and diffusion-controlled release from gelatin films[J]. International Journal of Pharmaceutics, 1984, 20(1): 87-98