大通牦牛肉质特性研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本试验选用年龄为18个月、成年发育正常、健康无病的大通牦牛各6头作为研究对象,利用氨基酸自动分析仪、固相微萃取器、气相色谱-质谱联用仪等先进仪器,对其产肉性能、食用品质、营养成分、脂肪酸组成、挥发性风味物质等方面进行了研究,结果表明:
     1.产肉性能
     18月龄大通牦牛宰前活重为142.80kg,胴体重73.5kg,胴体长为89.40cm,净肉重为54.24kg,屠宰率为47.91%,净肉率为37.1%,18月龄大通牦牛各项产肉指标均优于当地牦牛,其中宰前活重、胴体重、净肉重、眼肌面积极显著高于当地牦牛(p<0.01),这与大通牦牛是通过选育而形成的稳定的遗传性有关。大通18月龄牦牛宰前活重、胴体重、净肉重、与体尺指标呈显著正相关。
     2.食用品质
     18月龄牦牛的肉色、大理石纹评分均低于成年牦牛,二者大理石纹评分呈显著性差异(P<0.05)。18月龄牦牛肉的系水力、熟肉率均高于成年牦牛肉,二者系水力呈显著性差异(P<0.05),熟肉率呈极显著性差异(P<0.01)。18月龄牦牛肉的失水率、肌纤维直径均低于成年牦牛肉,二者的失水率呈显著性差异(P<0.05),肌纤维直径呈极显著性差异(P<0.01)。18月龄牦牛肉和成年牦牛肉的pH1、pH24相当,差异不显著。18月龄牦牛肉贮存损失率略高于成年牦牛,无显著差异。
     3.营养成分
     18月龄牦牛肉蛋白质含量比成年牦牛肉高0.61%,18月龄牦牛肉脂肪含量比成年牦牛低0.96%。18月龄牦牛肉氨基酸总量(TAA)为80.14g/100g,比成年牦牛肉低3.14g/100g,其中18月龄牦牛肉必需氨基酸(EAA)含量为39.75g/100g,比成年牦牛肉低1.73 g/100g,缬氨酸为4.27g/100g,高于成年牦牛肉0.10g/100g。与联合国粮农组织(FAO)提出的理想蛋白质中的必需氨基酸含量比较,18月龄牦牛肉中的蛋氨酸+胱氨酸(Met+Cys)、色氨酸(Trp)含量明显低于理想蛋白质中相应氨基酸的含量,赖氨酸(Lys)含量则明显高于理想蛋白质中相应氨基酸的含量。
     4.脂肪酸组成
     18月龄牦牛肉肌间饱和脂肪酸比成年牦牛肉低9.44%差异极显著。18月龄牦牛肉肌间单不饱和脂肪酸比成年牦牛肉高15.75%。18月龄牦牛肉肌间多聚不饱和脂肪酸比成年牦牛肉低0.89%。其中18月龄牦牛肉中共轭亚油酸(c9t11C18:2)、共轭亚油酸(t10c12C18:2)含量分别为1.91%、1.89%均低于成年牦牛肉2.01%、1.92%的含量。18月龄牦牛和成年牦牛p:s分别为0.63、0.53, 18月龄牦牛和成年牦牛n-6/n-3PUFA分别为2.40、2.23均在推荐比例范围内。
     5.挥发性风味物质
     18月龄牦牛肉的风味物质与成年牦牛肉大致相同,数量比成年牦牛肉共少7种,其中烷烃少1种,烯烃少1种,醇少1种,酮少1种,芳香族少1种,噻唑类少1种,噻吩类少1种。
Six normal,healthy 18-months-old and adult Datong yaks selected randomly were taken for the reseach The meat performance ,the edible quality ,the nutriet contents,the fatty acid components and the volatile flavor substance in the yaks were detected systematically .After the research and the analysis,the results were shown as below:
     1. Meat performance
     Compared with local yak,Datong yak’s meat performance is better.Live weight of Datong yak is142.80kg, flesh weight is73.5kg, carcass length is 89.40cm, really flesh weight is 54.24kg, slagther rate47.91 %,really flesh rate is 37.1%.There are items that are significant highter than local cattles’(P<0.01). There were significant relationships(P<0.05)between live weight、the flesh weight、the really flesh rate and the indicators of body size
     2. Edible quality
     The color and marble of 18-months-old yak meat are less than those of adult yak meat,and marbe was the significance difference(P<0.05). The 18-months-old yak meat quality contributed to water holding capacity(P<0.05),the cooked meat ratio(P<0.01)higher than those of the adult yak.,and the lose water ratio,the muscle fiber diameter were lower than those of the adult yak, which indicated that the quality of the 18-months-old beef meat was better than adult yak meat’s.
     3. Nutrient contents
     Nutrient contents of 18-months-old yak meat contributed to water protein content compared as those of adult yak meat more than 0.61%,and the fat content as the adult yak less than 0.96%. Amino acid total quantity(TAA) of calf yak is 80.14g/100g less than adult yak, essential amino acids(EAA) is 39.75g/100g less than adult yak. According to the UN Food and Agriculture Organization(FAO),proposed the content mode of the ideal protein's essential amino acids(EAA),the18-months-old yak meat's Met+Cys、Trp were lower than those of ideal pattern,the content of Lys is higher than the ideal pattern.
     4. Fatty acid components
     18-months-old yak’s intermuscular saturated fatty acid and Polyunsaturated fatty acid were less than adult yak,there are 9.44%,0.89%. 18-months-old yak’s Monounsaturated fatty acid is more than adult yak 15.75%.The p:s of calf yak is 0.63,the p:s of adult yak is0.53.
     5. Volatile flavor substances
     Yak meat volatile flavor ingredients are approximately same,the 18-months-old yak meat’s total amount less than 7 kinds of those of adult yak,which include alkane 1 kind,alkene 1 kind,mellowly1 kinds,alkone1kind,the aromatic 1 kind,furane 1 kind.
引文
[1]姬秋梅.中国牦牛品种资源的研究进展[J].自然资源学报,2001,16(6):565-570.
    [2]周生明.甘肃牦牛产业发展现状与前景[J].中兽医医药杂志,2003,1(3)43-44
    [3]黄筱声.高原美味雪域珍品)耗牛食品前景广阔[J].中国乳品工业20008, 28(:0)14-16.
    [4]秋梅.中国牦牛品种资源的研究进展[J].自然资源学报,2001,16(6):565-570.
    [5]钟光辉,等.九龙牦牛肉质特性的研究[J].中国牦牛,1995,(2):30-32.
    [6]柏家林,王敏强,等.大通牦牛的培育—6月龄、18月龄产肉性能的研究[J].草与畜杂志,1998(4): 21-22.
    [7]黄友鹰.加速发展中国西部青藏高原牦牛畜产品加工业的探讨及对策[J].西南民族学院学报,2001,(2):221-224.
    [8]陆仲璘,何晓琳,阎萍.世界上第一个牦牛培育新品种—“大通牦牛”简介[J].中国草食动物,2005,(5).
    [9]陈晓德,李鸿康,等.青海省大通种牛场牦牛产业化现状及发展建议[J].青海畜牧兽医杂杂志,2005,35(1):25-25.
    [10]姬秋梅,张永清.西藏三大优良类群牦牛的产肉性能及肉品质分析[J].试验研究,2000(5):21~25.
    [11]王建民,高秀华.优质牛肉品质的研究[J].草食家畜,1995.(4):15~19.
    [12]王树林.不同体重牦牛犊产肉性能和肉品质分析的研究[J].草食家畜,2003,(1):61-63.
    [13]李鹏.甘南牦牛肉用品质、血清生化指标及其相关性的研究[D].兰州:甘肃农业大学, 2006.
    [14]丁凤焕.牦牛、犏牛及黄牛肉脂肪酸和风味物质测定及生产性能的比较分析[D].西宁:青海大学,2008.
    [15]P.J.Toscasa.Partial least squares(PLS)regression for the analysis of instrument.Measurement ndsensory meat quality data[J].Meat Science,1999,52:173-78.
    [16] S.A.Chowdhury.Meat producion characteristics of black bengal goat[J]. Animal Science, 2004,17:848-856.
    [17]胡宝利.不同年龄秦川牛胴体性状与肉质性状的研究[D].西安:西北农林科技大学,2001.
    [18]杨博辉,姚军.大通牦牛肌肉纤维组织学特性研究[J].中国草食动物,2001,(5):34-35.
    [19]曾勇庆,孙玉民.鲁西黄牛与利鲁杂交牛肉质特性的研究[J].黄牛杂志,1999,(3):23-25.
    [20]韩玲.白牦牛产肉性能及肉质测定分析[J].中国食品学报,2002(4):30-34.
    [21]戴瑞彤,杨龙江,吴国强.肉类质量的研究进展.肉类研究[J],2000(2):11-13.
    [22] G.Renand,B.Picard.Relationships between muscle characteristics and meat quality traits of young Charolais bulls[J].Meat Science,2001,59:49-60
    [23]Wood J D.,R.I.Richardson.Effects of fatty acids on meat quality:a review[J].Meat Science,2003,66:21-32.
    [24]R.E.Klont.Muscle fibre type and meat quality[J].Meat Science,1998,49:219-229.
    [25]周光宏,刘丽.牛肉等级评定方法和标准.[J].垮类工业2001(6):41-48.
    [26]张辉,雷风.牦犊牛肉营养成分测定[J].青海畜牧兽医杂志,1997,(4):23-24.
    [27]《麦洼耗牛群体选育》项目组.麦洼耗牛肉用特性研究[J].四川草原,1995,(3):36-37.
    [28]严昌国,王勇.延边黄牛牛肉品质特性的研究[J].黄牛杂志2004,30(3):5-7.
    [29]罗应荣,朱化彬,张之宣.皮埃蒙特杂交牛与鲁西黄牛牛肉营养成分对比[J].黄牛杂志,1999,(4):89~83.
    [30]邹文辉,雷引莲.青海省果洛牦牛产肉性能研究[J].黄牛杂志,2001,(1):27-28.
    [31]葛长荣,田允波,陈韬,伍勇.大额牛肉质特性研究[J].肉类研究,1995,(5):25-27.
    [32]焦小鹿,刘海珍,等.青海牦牛肉的营养品质分析[J].畜产品2005,25(3):57-58.
    [33]余群力,蒋玉梅,等.白牦牛肉成分分析及评价[J].中国食品学报2005,5(4):124-127.
    [34]田永全.脂肪酸的营养功能[J].中国食品与营养.2007,(8):12-13.
    [35]GermanJ.B.Butyrieaeid:aroleineaneerprevention.[J].NutrBull,l999,24:293-299
    [36] KeysA,MenottiA,KarVonenMJ, etal.The died and 15-year death rate in the seven countries study [J]. AMJEPidemionl,1986,124(6):903-915.
    [37] HuFB,StamPfer MJ. etal.Dietary fatintake and the risk of coronary heart disease in women [J]. New EnglJMed,1997.337(21):1491-1499.
    [38]BurtonA.Eoncolyticeffeetsoffattyaeidsinmieeandrats[J].AmJClinNutr,1991(5)1082S-1086S.
    [39]吴时敏.功能性油脂[M].北京中国轻工业出版社,2001,82-110.
    [40]Burdge G C,JonesAE,Wbotton5A.Eieosapentaenoie and do cosapentaenoic acids are the principal products of alpha-linolenic acid metabolism in young men[J].Br J Nutr.2002,88(4):355-364.
    [41]孙远明,余群力主编.食品营养学[M].中国农业大学出版社,2002,55-60.
    [42]徐国珍.黑山羊脂肪酸成分测定及营养价值评价[J].山地农业生物学报,1998,(5):304-306.
    [43]束彤.高原地区食物中脂肪和脂肪酸的分析研究[J].食品科学,1995,(5):52-55.
    [44]蔡妙颜,李冰,袁向华.膳食中脂肪酸平衡[J].粮油食品科技,2003,(2):37-39.
    [45]李长忠,李清香.皮尔蒙特牛肉化学组成及肉品品质[J].肉类科学,2004,(5):31-34.
    [46]徐秀兰等.脂肪酸的分析方法[J].生物技术,1998,(3):161-65.
    [47]陈银基,周光宏,鞠兴荣.低剂量γ辐照对牛肉肌内脂肪酸组成及牛肉质量的影响[J].食品科学,2008,(7):81-86
    [48]Pariza MW et al. Cancer Res, 1983, 43(Suppl):2444s-2448s.
    [49]Ha YL.Carcinogenesis,1987,8:1881-1887.
    [50]Grnevra Lombardi_Boccia,Sabrna Lanzir.Aspects of meat quality:trace elements and B vitamins in raw and cooked meats[J].Journall of Food Composition and Analysis.2005,18:39~46.
    [51]张云琦.共轭亚油酸及其来源[J].饲料博览,2003,(2):8-10.
    [52]王中华.反刍动物挥发性脂肪酸中间代谢[[J],草食家畜,1995,(2):23-32.
    [53]李宏涛.气相色谱法快速测定大豆油中脂肪酸含量[J].药物分析杂志,1995,(6):27-30.
    [54]刘晋平,武培升.奶山羊肉脂肪酸和氨基酸含量分析[J].肉品卫生,1996,(8):12-14
    [55]徐秀兰,胡磊军,吴梧桐.脂肪酸的分析方法研究[J].药物生物技术,1998,(3):163-165.
    [56]魏法山.固始鸡部分肉质性状动态研究[D].河南:河南农业大学,2005.
    [57] Mottram,D.S.Flavour formation in meat and meat products:A review[J]. FoodChemistry, 1998, 62(4):415-424.
    [58]张伟力.猪肉的风味[J].养猪,2003,2:47-50.
    [59]张春晖.发酵香肠成熟前后挥发性成分的固相微萃取-GC-MS分析[J].分析测试学报,23 (6):40-43.
    [60]王存堂.天祝白牦牛肉质特性的研究[D].兰州:甘肃农业大学,2006.
    [61]Wang Q, Wu J P, Zhang S G, et al. Fatty acid composition analysis from the kidney of yak by Gas-Chromatography[J]. Chromatographia,DOI:10.1365/s10337-008-0845-6.
    [62]陈国顺.子午岭野家杂种猪和合作猪肉质特性比较及风味挥发性成分的提取与分析[D].兰州:甘肃农业大学,2004.
    [63]陆仲磷,王敏强等大通牦牛的培育[J].草食动物,21(3):23-24.
    [64]刘长英.甘南藏羊肉用品质及血液生化指标的研究[D].兰州:甘肃农业大学,2007. [65」王明雯,等.以肉的pH值鉴定肉的品质[J].河南畜牧兽医,2001,(4):3-32.
    [66]罗毅豪.青海大通犊牦牛肉品质特性分析及安全性研究[D].西宁:青海大学,2008.
    [67]毛学荣.欧拉型藏羊的肉质分析[J].青海畜牧兽医杂志.2005,(3):4-7
    [68]Ludden,P.A.,Kerley,M.S.Amino acid and energy interrelationships in growing beef steers:II.Effects of energy intake and metabolizable lysine supply on growth. [J] Anim Sci.76(1998)3157-3168.
    [69]Uhe,A.M.,Collier,G.R.,O'Dea,K.A comparison of the effects of beef,chicken and fishprotein on satiety and amino acid profiles in lean male subjects. [J] Nutr.122(1992)467-472.
    [70]王杰等.藏山羊肉的品质研究[J].四川草原,2000,(2):50-53.
    [71]束生庆.高原地区动物性脂肪、脂肪酸含量的分析[J].高原医学杂志,1996(3),60-61.
    [72]KEYS A,EFSON J T,GRANDE F.Serum cholesterol response to changes in the diet[J]. Etabolisan, 1965, 36:776-787.
    [73]JANA KRAFT,JOHN K.G.KRAMER,FRIEDRICH SCHOENE,JIM R.,etal.Extensive Analysis of Long-Chain Polyunsaturated Fatty Acids,CLA,trans-18:1 Isomers,and plasmalogenic Lipids in Different Retail Beef types. [J]Agric Food Chem.55(2008)1849-1911.
    [74] NEILJ STONE,ROBERTKUSHNER. Effects of dietarymo dification and treatment of obesity: emphasison improving vascularoutcomes[J].MedicalClinics 2008,(84):95-122
    [75]杨明,龙虎,文勇立,等.四川牦牛、黄牛不同品种肌肉脂肪酸组成的气相色谱-质谱分析[J].食品科学,2008,29(3):444-449.
    [76]王存堂,蒋玉梅,李鹏,韩玲.天祝白牦牛肉挥发性风味成分的SPEM/GC/MS测定[J].甘肃农业大学学报,2006,41(6):118-121.
    [77]吴冀华,裘爱泳.共轭亚油酸的分析[J].中国油脂,2002,27(2):177-178.
    [78] CAMERONND,ENSERM,NUTEGR,et al,Genotype with nutrition interaction onfattyacid coposition of intremusculerfat and relationship with flavour of pigmeat[J].Meat Science,2000,55(2):187-195.
    [79]WOOD J D,ENSERM,FISHERAV,et al.Fat deposition,fattyacid composition and meatquality:Areview[J].Meat Science,2008,78:343-358.
    [80]Gillis,M.H.,Duckett,S.K.,Sackmann,J.R.Effectsof supplemental rumen-protected conjugated linoleic acid or corn oil on fatty acid composition of adipose tissues in beef cattle[J]. Anim Sci.82(2004)1419-1427.
    [81]蔡妙颜,袁向华.膳食中的脂肪酸平衡[J].粮油食品科技.2003,11(2):37-39.
    [82]Mottram D S,Madruga M S,Some novel meatlike aromacompounds from the reactions oflkanedionesh hydrogensulfide and furanthiols[J].Agric.Food Chem.,1995,43(1):189-193.
    [83]Ponnampalam,E.N.,Mann,N.J.,Sinclair,A.J.Effect of feeding systems on omega-3 fatty acids,conjugated linoleic acid and trans fatty acids in Australian beef cuts:potential impact on human health.Asia Pac [J].Clin Nutr.15(2006)21-29.
    [84]Cai,L.,Koziel,J.A.,Davis,J.,Lo,Y.C.,Xin,H.Characterization of volatile organic compounds and odors by in-vivo sampling of beef cattle rumen gas,by solid-phase microextraction,and gas chromatography-mass spectrometry-olfactometry.[J]AnalBioanalChem.386(2006)1791-1802.
    [85]杨富民.肉用杂种一代羊肉品质特性研究[D].兰州:甘肃农业大学,2004.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700