生姜对全脑缺血再灌注大鼠脑组织炎性细胞因子和黏附分子的影响
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摘要
目的:观察生姜水提物对大鼠全脑缺血再灌注炎症级联反应的影响。
方法:SD雄性大鼠随机分为假手术组、模型对照组、尼莫地平组和生姜水提物组,灌胃给药,每天1次,共6次。末次给药后1h,采用Pulsinelli’s四动脉阻断法造成大鼠全脑缺血10min再灌注3h损伤模型(CIR)。酶联免疫吸附法(ELISA)测定脑组织白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)和细胞间黏附分子-1(ICAM-1)、血管细胞间粘附分子-1(VCAM-1)、内皮细胞选择素(E-selectin)、血小板选择素(P-selectin)含量。
结果:与假手术组比较,模型对照组大鼠脑组织TNF-α、ICAM-1、P-selectin显著升高(P<0.05);其他炎性细胞因子(IL-1β、IL-6、IL-8)和黏附分子(VCAM-1、E-selectin)均有不同程度的升高,但无统计学意义(P>0.05)。生姜水提物能明显降低大鼠脑组织TNF-α、ICAM-1、P-selectin含量(P<0.05～0.01),对其他炎性细胞因子(IL-1β、IL-6、IL-8)和黏附分子(ICAM-1、VCAM-1、E-selectin)均有不同程度的降低作用,但无统计学意义(P>0.05)。
结论:炎性细胞因子与黏附分子及由此介导的炎症级联反应参与缺血再灌注性脑损伤的病理过程,生姜水提物治疗脑缺血再灌注损伤的机制之一与降低炎性细胞因子与黏附分子含量、抑制炎症级联反应有关。
[Objective] To observe the change of inflammatory cytokines and adhesion molecules content after cerebral ischemia/reperfusion (CIR) and the effect of aqueous extract of ginger.
[Methods] Male SD rats were evenly randomized into sham-operation, model, nimodipine, aqueous extract of ginger groups. CIR model was produced by Pulsinelli’s“Four Vessel Occlusion”method. The contents of inflammatory cytokines including interleutin-1β(IL-1β),interleutin-6(IL-6),interleutin-8 (IL-8) and tumor necrosis factor–α(TNF-α) and adhesion molecules including intercellular cellular adhension molecule (ICAM-1), vascular cellular adhension molecule (VCAM-1), endothelium selectin (E-selectin) and platelet selectin (P-selectin) in brain tissue were detected by enzyme-linked immunosorbent assay(ELISA). [Results] Compared with sham-operation group, the contents of TNF-α、ICAM-1
and P-selectin in brain tissue of model group increased significantly (P<0.05) . While compared with model group, aqueous extract of ginger could decrease the contents of TNF-α,ICAM-1 and P-selectin in brain tissue(P<0.05～0.01), but no significant difference to the content of IL-1β,IL-6,IL-8, ICAM-1,VCAM-1 and E-selectin, compared with model control group.
[Conclusion] The therapeutic action of aqueous extract of ginger on cerebral ischemia reperfusion can be realized by decrease the inflammatory response induced by cytokines and adhesion molecules.
方法:SD雄性大鼠随机分为假手术组、模型对照组、尼莫地平组和生姜水提物组,灌胃给药,每天1次,共6次。末次给药后1h,采用Pulsinelli’s四动脉阻断法造成大鼠全脑缺血10min再灌注3h损伤模型(CIR)。酶联免疫吸附法(ELISA)测定脑组织白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)和细胞间黏附分子-1(ICAM-1)、血管细胞间粘附分子-1(VCAM-1)、内皮细胞选择素(E-selectin)、血小板选择素(P-selectin)含量。
结果:与假手术组比较,模型对照组大鼠脑组织TNF-α、ICAM-1、P-selectin显著升高(P<0.05);其他炎性细胞因子(IL-1β、IL-6、IL-8)和黏附分子(VCAM-1、E-selectin)均有不同程度的升高,但无统计学意义(P>0.05)。生姜水提物能明显降低大鼠脑组织TNF-α、ICAM-1、P-selectin含量(P<0.05～0.01),对其他炎性细胞因子(IL-1β、IL-6、IL-8)和黏附分子(ICAM-1、VCAM-1、E-selectin)均有不同程度的降低作用,但无统计学意义(P>0.05)。
结论:炎性细胞因子与黏附分子及由此介导的炎症级联反应参与缺血再灌注性脑损伤的病理过程,生姜水提物治疗脑缺血再灌注损伤的机制之一与降低炎性细胞因子与黏附分子含量、抑制炎症级联反应有关。
[Objective] To observe the change of inflammatory cytokines and adhesion molecules content after cerebral ischemia/reperfusion (CIR) and the effect of aqueous extract of ginger.
[Methods] Male SD rats were evenly randomized into sham-operation, model, nimodipine, aqueous extract of ginger groups. CIR model was produced by Pulsinelli’s“Four Vessel Occlusion”method. The contents of inflammatory cytokines including interleutin-1β(IL-1β),interleutin-6(IL-6),interleutin-8 (IL-8) and tumor necrosis factor–α(TNF-α) and adhesion molecules including intercellular cellular adhension molecule (ICAM-1), vascular cellular adhension molecule (VCAM-1), endothelium selectin (E-selectin) and platelet selectin (P-selectin) in brain tissue were detected by enzyme-linked immunosorbent assay(ELISA). [Results] Compared with sham-operation group, the contents of TNF-α、ICAM-1
and P-selectin in brain tissue of model group increased significantly (P<0.05) . While compared with model group, aqueous extract of ginger could decrease the contents of TNF-α,ICAM-1 and P-selectin in brain tissue(P<0.05～0.01), but no significant difference to the content of IL-1β,IL-6,IL-8, ICAM-1,VCAM-1 and E-selectin, compared with model control group.
[Conclusion] The therapeutic action of aqueous extract of ginger on cerebral ischemia reperfusion can be realized by decrease the inflammatory response induced by cytokines and adhesion molecules.
引文
[1] 王维治,主编.神经病学[M].第五版.北京:人民卫生出版社,2004,8:126
[2] 王建枝.脑功能不全. 陈修,陈维洲,曾贵云主编.心血管药理学[M].第三版.北京:人民卫生出版社,2003.236-258
[3] 方舒东,朱也森.脑缺血再灌注损伤的病理生理研究进展[J].医学综述,2006 ,12 (18):1114-1116
[4] 刘启锋,刘明,刘玉河,等.脑缺血再灌注损伤机制研究进展.中华神经外科疾病研究杂志[J],2006;5(6):566-568
[5] 韩敏,刘艳凯,薛茜.脑缺血再灌注损伤的研究进展[J].河北北方学院学报(医学版), 2006,23(5):75-78
[6] Ritter LS, Orozco JA, Coull BM, et al. Leukocyte accumulation and hemodynamic changes in the cerebral microcirculation during early reperfusion after stroke [J]. Stroke, 2000, 31(5):1153-1162.
[7] Danton GH, Dietrich WD. Inflammatory mechanisms after ischemia and stroke [J]. Neuropathol Exp Neurol, 2003, 62(2):127-137.
[8] DelZoppo GJ, Becker KJ, Hallenbeck JM. Inflammation after stroke: is it harmful [J]. Arch Neurol, 2001, 58(4):669-673.
[9] Fyijins CJM, Kappelle LJ. Inflammatory cell adhesion molecules in ischemic cerebral vascular disease[J]. Stroke, 2002, 33 (8): 2115-2123.
[10] Zimmerman GA, McIntyre TM, Mehra M, et al. Endothelial cell-associated intercellular adhesion [J]. J Cell Bio, 1990, 110(2): 529-540
[11] 朱道银.细胞因子.沈关心,王明丽,朱道银,等.微生物与免疫学[M].第5版.北京:人民卫生出版社,2003.53-57
[12] 黄启福,王元勋,卫洪昌,等.病理学.北京:科学出版社.2004,4:37-45
[13] 张娜,朱晓磊,李澎涛,等.胆酸、栀子苷及配伍对大鼠缺血再灌注脑组织TNF-α、IL-1β和ICAM-1含量的影响[J].中国医药学报,2003,18(8):463。
[14] Zhai Q H, Fut rell N, Chen FJ, et al. Gene exp ression of IL-10 in relationship to TNF-alpha, IL-1beta and IL-2 in the rat brain following middle cerebral artery occlusion [J]. Neurol Sci, 1997, 152 (2):119 - 124.
[15] 杨养贤,延卫东,乔晋,等.黄芩苷对大鼠缺血再灌注脑组织TNF-α、IL-1β表达的影响[J]. 西安交通大学学报( 医学版),2005, 26(3):220-224
[16] Barone FC, Arvin B, White RF, et al. Tumor necrosis factor - α:A mediator of focal ischemic brain injury[J ] . Stroke, 1997, 28 (6):1233 - 1244.
[17] Nawashiro H, Martin D, Hallenbeck JM. Neuroprotective effects of TNF binding proteinin focal cerebral ischemia[J]. Brain Res, 1997, 778(2):265 - 271.
[18] Shohami E, Bass R, Wallach D, et al. Inhibition of tumor necrosis factor - α( TNF - α) activity in rat brain is associated with cerebro protection after closed head injury[J]. Cereb Blood Flow Metab, 1996, 16(3): 378 - 384
[19]曹晓岚,任丽,臧运华.溶栓颗粒对脑缺血再灌注大鼠的脑保护作用研究[J].中西医结合心脑血管病杂志,2005,3(1):37-38
[20]陈寿权,王万铁,王明山,等.醒脑静对家兔脑缺血再灌流时TNF、IL - 1β、IL - 6 水平及脑超微结构影响的实验研究[J].中国急救医,2000,20(11):637-639
[21] 卢传坚,欧明,王宁生. 姜的化学成分分析研究概述[J]. 中药新药与临床药理,2003,14(3):215-217.
[22] 何文珊,严玉霞.生姜的化学成分与生物活性研究概况[J].中药材,2001, 24(5):376-379.
[23] 药理教研组. 鲜姜注射液抗炎消肿及镇痛作用实验研究[J]. 泸州医学院学报,1979,(3):1-3
[24] 张竹心. 生姜油的抗炎作用研究[J]. 中草药,1989;20(12):545-546.
[25] Penna SC, Mederios MV, Aimbire FS, et al. Anti-inflammatory effect of the hydralcoholic extract of Zingiber officinle rhizomes on rat paw and skin edema[J]. Phytomedicine, 2003, 10(5): 381-385.
[26] 王梦,钱江美,苏简单. 干姜乙醇提取物解热镇痛及体外抑菌作用研究[J]. 中药新药与临床药理,2003,14(5):299-301
[27] Ojewole JA. Analgesic, antiinflammatory and hypoGlycaemic effects of ethanol extract of Zingiber officinale (roscoe) rhizomes (zingiberaceae) in mice and rats [J]. Phytother Res, 2006, 20(9):764-772.
[28] 末川守. 关于生姜药理学的研究(第Ⅳ报).(6)-姜醇对于花生四烯酸代谢的作用[J]. 国外医学中医中药分册,1987,9(3):160-162.
[29]Grzanna R,Phan P,Polotsky A, et al. Ginger extract inhibits beta-amyloid peptide-induced cytokine and chemokine expression in cultured THP-1 monocytes [J]. Altern Complement Med, 2004, 10(6):1009-1013.
[30]徐叔云,卞如濂,陈修. 药理实验方法学[M]. 第 3 版. 北京:人民卫生出版社. 2002. 1065-1066.
[31]蔡飞,李彩蓉,吴基良,闵清,等.茶黄素对大鼠脑缺血再灌注炎性细胞因子的影响[J]. 中药药理与临床 2007,23(1):23-24
[32]李建兵,张静,吴纯慧.肿瘤坏死因子TNF-α与缺血性脑血管病相关性研究进展[J].淮海医药, 2005, 23(1): 86 -87
[33]罗正曜.休克. 杨惠玲,潘景轩,吴伟康主编.高级病理生理学[M].第二版.北京:科学出版社. 2006,7:106-121-129。
[34] Lavin SD, Hofman FM, Zlokovic BV. Circulating antibodyagainst tumor necrosis factor - αprotects rat brain from reperfusion injury[J]. J Cereb Blood Flow Metab, 1998, 18 (1):52 - 58.
[35]Clemens JA, Stephenson DT, Smalsting EB, et al. Global ischemia activates nuclear factor - KB inforebrainneurons ofrats [J]. Stroke, 1997, 28 (5): 1073 - 1081.
[36]Siren Al, Liu Y, Feuerstein G, et al. Increased release of tumor necrosis factor - αinto the cerebrospinal fluid and peripheral circulation of aged rats [J]. Stroke, 1993, 24(6): 880 - 888.
[37] Elneihoum AM, Falke P, Axelsson l, et al. Leukocyte activiation detected by increased plasma levels of inflammatory mediators in patients with ischemic cerebro vascular diseases [J]. Stroke, 1996, 27 (10): 1734 - 1738.
[38]霍则军,张莉,钱瑞琴.针刺不同穴组对全脑缺血再灌注大鼠TNF-α、IL-6 、WBC和自由基的影响[J],针刺研究, 2003,28(2):94-98
[39]高晶,郭玉璞.缺血后脑组织损伤中的炎细胞作用[J].中华神经科杂志,1999,32: 303-305
[40]郭壮丽,裴海涛. 急性脑缺血再灌注大鼠脑内IL-1β蛋白表达及针刺对其抑制作用的研究[J]. 中华物理医学与康复杂志, 2005, 27 (1):16-19
[41]吕洋, 沈维高, 刘国辉.脑缺血再灌注损伤中IL-1β的作用及机制[J]. 北华大学学报(自然科学版), 2004,5(6): 524-528
[42]宋月英,齐刚,韩景田,等. 大鼠全脑缺血再灌注损伤后脑内白细胞介素-1β 变化及红景天苷的影响[J]. 中国临床康复,2006,10(11):30-32
[43]Pera J, Zawadzka M, Kaminska B, etal. Influence of chemical and ischemic preconditioning on cytokine expression after focal brain ischemia[J]. Neurosci Res, 2004, 78(1):132-40
[44]李静,明亮,黄茸茸,等.黄芪提取物对大鼠全脑缺血再灌注后IL-1β、TNF-α和IL-6表达的影响[J].安徽医科大学学报,2005,40(6):512-514
[45] Relton JK, Martin D, Thompson RC et al. Peripheral administration of Interleukin-1 receptor antagonist inhibits brain damage after focal cerebral ischemia in the rat[J] . Exp Neurol , 1996,138 (2) :206～13
[46] Beamer NB, Coull BM, Clark WM, et al.Interleukin-1 and interleukin-6 receptor antagonist in acute stroke[J]. Ann Neuro , 1995,37 (6) :800～5
[47] Hara H, Fink K, Moskowitz MA ,et al. Attenuation of transient focal cerebral ischemicinjury in transgenic mice expressing a mutant ICE inhibitory protein[J]. Cereb Blood Flow Metab , 1997, 17(4) :370～5
[48]韦登明,黄光照,张益鹄,等.雷公藤内酯醇抑制大鼠脑局灶性缺血再灌注后脑组织IL-1βmRNA 表达的研究[J].中国药理学通报, 2003, 19(11): 1277～1280
[49]史朗峰,任惠民,白蓉,等. 国产降纤酶对缺血/再灌注大鼠脑组织TNF-α及IL-1βmRNA 表达的影响[J]. 中风与神经疾病杂志,2004, l-1( 6):544-546
[50]王贵林,朱路.生姜油的抗炎作用[J].中药药理与临床,2006,22(5):26-28。
[51]季永侠,袁荣峰.VD大鼠血清及脑脊液中IL-6水平变化[J].中国实用医药杂志, 2007, 2 (9): 28-29
[52]崔丽,郑惠民.白细胞介素-6与缺血性脑损伤.国外医学脑血管疾病分册[J],2002,10(2):134-136
[53]雷秀珍,李秀英.异丙酚对大鼠局灶性脑缺血/再灌注损伤期炎性细胞因子的影响[J].重庆医科大学学报, 2004, 29(5): 622-625
[54]张泽舜. 细胞因子在脑外伤早期的变化和对人胚神经干细胞的影响[J].中华神经外科杂志, 2003,8(5): 233 -235
[55] Ahn MJ,Sherwood ER,Prough DS,et al.The effect of traumatic brain injury on cerebral blood flow and brain tissue nitric oxide levels and cytokine expression[J]. Neurotrauma, 2004, 21(10): 1431 - 1442.
[56]郭宗泽,冷汝溥,李光宇.IL-6和TNF-α在弥漫性脑损伤大鼠额叶的表达及血清中含量变化[J]. 解剖科学进展,2006,12(4):311～313
[57]向赟,鲁雄兵,许晓莉,等.大鼠脑缺血-再灌注后脑脊液及脑皮质海马TNF-α与IL-6的变化[J].中国康复,2004,19(3):139-141
[58]钟德桥,李少华,高观月,等.大鼠急性脑缺血及再灌注时白细胞介素(IL - 6、IL - 8) 的变化及意义[J]. 江汉大学学报(医学版), 2002,30(2):11-12
[59]王贵林,朱路,张渊.生姜油对佐剂性关节炎小鼠关节液前列腺素E2的影响[J].长江大学学报(自科版),2006,3(4):245- 248
[60]韩英,刘楠,陈荣华,等.白细胞介素8在大鼠脑缺血再灌注损伤中的变化[J].中国动脉硬化杂志,2005, 13(2): 155- 157
[61] Harada A , Sekido N , Akahoshi T, Wada T, Mukaida N , Matsushima K. Essential involvement of interleukin-8 (IL-8) in acute inflammation[J]. LeukoeBiod, 1994, 56 (5): 559-564
[62] Schroder JM, Mrowitz U, Christophers E. Purification and partial biologic char acterization of human lymphocyce-derived peptide with potent neutrophil-stimulating activity[J]. Immunol, 1988, 140(10): 534-540
[63]Kostulas N , Kividakk P, Huang Y, Matusevicius D, Kostulas V, Link H. Ischemic stroke is associated with a systemic increase of blood mononuclear cells expressing interleukin-8 mRNA [J]. Stroke, 1998, 29(2): 462-466
[64] Yamasaki Y, Matsuo Y, Matsuura N , Onodera H , Itoyama Y, Kogure K. Transient increase of cytokine-induced neutrophil chemoattactant , a member of the interleukin-8 family , in ischemic brain areas after focal ischemia in rat[J]. Stroke, 1995, 26 (2): 318-323
[65]刘士福.大鼠脑缺血再灌注损伤血清IL-8和中性粒细胞趋化指数的变化[J]. 放射免疫学杂志,2006,19(2):116-118
[66] Beech JS, Reckless J, Mosedale DE, et al. Neuroprotection in ischemia-reperfusion injury: an anti-inflammatory approach using a novel broad-spectrum chemokine inhibitor[J]. Cereb Blood Flow Metab, 2001, 21 (6): 683-689
[67]Aloisi F, Car F, Borsellino G. Production of hemolymphopoietic cytokines (IL-6, IL-8, colonystimulating factors) by normal human astrocytes in response to IL-1βand necrosis factor - α[J]. Immunol, 1992, 149: 2358
[68]Hofman FM, Chen P, Jeyaseelan R, et al. Endothelin-1 induces production of the neutrophil chemotactic factor interleukin-8 by human brain -derived endothelial cells[J]. Blood, 1998, 92: 3064
[69]刘继明,朱兴国,朱华亭,等. IL-8 单抗保护兔肝脏免受中性粒细胞介导的再灌注损伤[J].上海免疫学杂志,2001,21(1):7-10
[70]曹秋云,江开达,潘旭东.大鼠脑缺血再灌注损伤 IL-8 变化的研究[J]. 放射免疫学杂志, 2001, 14(2): 123-125
[71]徐霖,李树浓.移植免疫.杨惠玲,潘景轩,吴伟康主编.高级病理生理学[M]. 第二版.北京:科学出版社. 2006,21:378-380。
[72]尹静,杨,李俐涛,等.粘附分子与缺血性脑损伤. 脑与神经疾病杂志[J],2007,15(4):318-320
[73]Zhang RL, Zhang ZG, Chopp M, et al. Thrombolysis with tissue plasminogen activator alters adhesion molecule expression in the ischemic rat brain [J]. Stroke, 1999, 30:624～629
[74]陈邦国 ,毛庆菊 ,孔立红.电针对局灶性脑缺血再灌注大鼠缺血脑区细胞黏附分子表达的影响[J].湖北中医学院学报,2006,8(4):3-5
[75] Danton GH, Diet rich WD. Inflammatory mechanisms after ischemia and stroke [J]. Stroke,2003,62(2):127～137
[76] Zhang RL, Chopp M, Zologa C, et al. The temporal p rofiles of ICAM-1 protein and mRNA expression after transient MCA occlusion in the rat [J] . Brain Res, 1995, 682:182-188.
[77]郝延磊,蒲传强,朱克,张凤英,刘洁晓.细胞间粘附分子-1 和 E-选择素在局部脑缺血中的作用[J].中华老年心脑血管病杂志 2000, 2(1):54-57
[78]路文革,白宏英,王建平.垂体腺苷酸环化酶激活肽对大鼠脑缺血再灌注后细胞间黏附分子表达的影响[J]. 中国实用神经疾病杂志,2007,10(2):53-56
[79]苏红梅,朱广旗, 欧阳泠星,等.针刺对大鼠脑缺血再灌注损伤后ICAM-1表达的影响[J].上海针灸杂志,2006,25(12):30-32
[80]魏慧利,刘亚娴.地圣珠水煎剂对脑缺血再灌注后炎症反应的影响[J].中药药理与临床,2007,23(5):192-193
[81]向红兵,晋光荣 ,吴颢昕 ,等.大鼠多发性脑梗死后脑组织内皮细胞ICAM-1表达的变化[J].东南大学学报( 医学版)2004,23(5):295-298
[82]罗成基, 裴雪涛主编. 造血微环境[M].北京:世界医药出版社,2001:1
[83]司英健,张曦,陈幸华,等. VCAM-1 和EGFP双基因共表达重组腺病毒的构建[J]. 重庆医学,2007,36(17):1699-1703
[84]张丽慧,魏尔清.ICAM-1、VCAM-1在脑缺血损伤炎症机制中的作用及调控[J]. 中国药理学通报,2005, 21(11):1281～4
[85]雷万龙,袁群芳,张怀波,等.粘附分子表达和巨噬细胞浸润在缺血皮质和基底节区分布的比较[J].广东解剖学通报,1998,20(2):9-12
[86]Selakovic V, ColicM, JovanovicM et al. Cerebrospinal fluid and plasma concentration of soluble intercellular adhesion molecule 1,vascular cell adhesion molecule 1 and endothelial leukocyte adhesion molecule in patients with acute ischemic brain disease [J].Vojnosanit Pregl, 2003, 60(2):139 - 46.
[87]Montaner J, Rovira A, Molina CA et al. Plasmatic level of neuroin flammatory markers predict the extent of diffusion weighted image lesions in hyperacute stroke [J]. Cereb Blood Flow Metab, 2003, 23(12):1403 - 7.
[88]Zhang LH, Wei EQ. Neurop rotective effect of ONO21078, a leukotriene receptor antagonist, on transient global cerebral ischemia in rats [J]. Acta Pharmacol Sin, 2003, 24: 1241 - 7.
[89]Sander WE, Wilson RW, Ballantyne CM, et al. Molecular cloning and analysis of in vivo expression of P- selectin[J]. Blood 1992, 80(3):795
[90]才立云,赵宝春,耿旭. 血管黏附分子中选择素结构、功能及其表达的特点[J].中国组织工程研究与临床康复,2007,11(14): 2729-27312
[91]陈志鸿,宋宝辉,巴雪青.选择素及其配体的研究进展[J].国外医学遗传学分册, 2005, 28 (5):270-274
[92]刘玉珍,韩景献,蒋戈利,等.电针对大鼠脑缺血再灌注模型P-选择素和E-选择素表达影响的实验研究[J].时珍国医国药,2007,18(1):79-81
[93]Chen Y, Ruetzler C, Pandipati S, et al. Mucosal tolerance to E-selectin provides cell- mediated protection against ischemic brain injury[J]. Proc Natl Acad Sci USA, 2003, 100(25):15107
[94]刘皖生,唐乙,郑余银,等. 脑缺血再灌注的炎症损伤分子机制及吲哚美辛的保护作用[J].解放军医学杂志,2007,32(10):1051-1053
[95]盛文利,李锐,许晓伟,等. E- 选择素介导的大鼠脑缺血再灌注后继发炎症损伤的实验干预[J]. 中山大学学报(医学科学版),2007,28(1):48
[96]张国红,王春霖,吕平,等.大鼠局灶性脑缺血再灌注后不同时间E-选择素、P-选择素和细胞间粘附分子-1的表达[J].中国药理学通报,2005,21(10): 1218～23
[97]HUANG J, CHOUDHRI T F, WINFREE C J, et al. Postischemic cerebrovascular E - selectin expression mediates tissue injury in murine stroke [J]. Stroke, 2000, 31 (12): 3047- 3053.
[98]Suzuk H, Abe K, Tojo S, et al. Post ischemic expression of P-selectin immunoreactivity in rat brain [J].Neuosci Lett,1997,228:151-154
[99]阎俊,周红.选择素与缺血性脑血管病[J].国外医学脑血管疾病分册.2005,13 (11): 857- 859
[100]曾丽莉,刘振国.P-选择素与缺血性脑血管病[J].上海第二医科大学学报,2002,22 (2): 182-185
[101]倪红,储照虎,刘新亚.选择素与缺血性脑卒中[J].神经疾病与精神卫生, 2006, 6(3): 227-230
[102]Yao L, Setiadi H, Xia IJ, et al. Divergent inducible expression of P- selectin and E- selectin in mice and primates [J]. Blood, 1999 , 94(11) : 3820～3828
[103]Thomas WS , Mori E , Copeland BR , et al.Tissue factor contributes to microvascular defects after focal cerebral ischemia [J ] 1 Stroke,1993 , 24 : 847～853
[104]曾杨滨,杨期东,彭光莹,等.可溶性E-和P-选择素与急性脑梗死的关系[J].中国急救医学,2000,20(12):715-716
[105]姚巍,陈天铎,李培杰,等.可溶性P-选择素、血小板激活因子与急性脑梗死关系. 中国临床神经科学,2002,10(1):28-30
[106]狄政莉,万琪,王洪典,等.选择素在大鼠全脑缺血再灌注微血管内皮细胞损伤中的作用[J]. 中风与神经疾病杂志, 2002,19(4): 194 - 96
[1]罗集鹏,张保国,赵冰清,等.生药学[M].北京:中国医药科技出版社.1996,10:154-157
[2]匡芮,匡轩,朱海涛.生姜的功能因子与医疗保健作用[J].中国食物与营养, 2007, (2): 52-53
[3]何文珊,严玉霞,郭宝江.生姜的化学成分与生物活性研究概况[J]. 中药材,2001;24(5): 376-379.
[4]Ahmed RS, Seth V, Pasha ST, et al. Influence of dietary ginger(Zingiber officinales Rosc) on oxidative stress induced by malathion in rats[J]. Food Chem Toxical, 2000,38(5):443-450
[5]Ahmed RS, Seth V, Baneijee BD, et al. Influence of dietary ginger(Zingiber officinales Rosc) on antioxidant defense system in rat: comparison with ascorbic acid[J]. Indian J Exp Biol,2000,38(6):604-606
[6] 刘金玲,王卫东,郭景云. 鲜生姜提取液抗氧化作用的探讨[J]. 河南中医, 1996;16(3):156.
[7] 柳乃奎,黄雪松.姜酮、脱氢姜酮、姜酚肟对两种自由基的清除作用[J]. 食品科学,2004, 25(6):169-172
[8] 沈 寻,丁华,武彩霞,等. 生姜有效部位对高脂血症大鼠的抗氧化作用研究[J].中国生化药物杂志, 2005,26(6): 330-332
[9] 黄雪松,王建华,路福绥.生姜抗氧化作用的研究[J].食品工业科技,1997, 4:16-17
[10]Ippoushi K, Azuma K, Ito H, et al. 6-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions[J]. Life Sci,2003;73(26):3427-37.
[11] 张关亭,王军,张磊,刘惠霞,张薇.生姜水提物对全脑缺血再灌注大鼠凝血功能的影响[J].中医研究,2007,20(4):18-20
[12] 李海平,刘宁,霍贵成.生姜的抗动脉粥样硬化作用[J].国外医学卫生学分册,2003, 30 (2): 98-101
[13]Srivastava KC. Isolation and effects of some ginger components on platelet aggregation and eicosanoid biosynthesis[J]. Prostaglandins Leukt Med, 1986; 25(2-3): 187-198.
[14]Srivas KC. Effects of aqueous extracts of onion, garlic and ginger on platelet aggregation and metabolism of arachidonic acid in the blood vascular system: in vitro study [J].Prostaglandins Leukot Med,1984, 13(2):227-235.
[15]Suekawa M, Yuasa K, Isono M, et al. Pharmacological studies on ginger. IV. Effect of (6)-shogaol on the arachidonic cascade [J].Nippon Yakurigaku Zasshi,1986,88(4): 263-269
[16]Srivastava KC. Effect of onion and ginger consumption on platelet thromboxane production in humans [J].Prostagl and Leukotr Essent Fatty Acids, 1989, 35(3):183-185.
[17]陈燕, 田泽, 葛毅强, 等. 姜油树脂调节血脂和抗血小板聚集作用的研究[J]. 营养学报[J],2001,23(4):338-341.
[18]高立波,姜波,李志坚,等. 姜总酮对血小板功能及血栓形成的影响[J]. 中国药理学通报[J],1996,12(3):288.
[19]Koo KL,Ammit AT,Tran VH,et al. Gingerols and related analogues inhibit arachidonic acid-induced human platelet serotonin release and aggregation [J].Thromb Res,2001,103(5):387-397.
[20]Nurtjahja-Tjendraputra E, Ammit AJ, Roufogalis BD, et al. Effectiveanti-platelet and COX-1 enzyme inhibitors from pungent constituents of ginger[J].Thromb Res,2003, 111(4-5):259-65.
[21]Kiuchi F, Shibuya M, Sankawa U. Inhibitors of prostaglandin biosynthesis from ginger [J]. Chem Pharm Bull (Tokyo),1982 Feb;30(2):754-757.
[22]卢传坚.姜对脑血管疾病的作用与途径[J].中国临床康复,2005,9(45):187 –189
[23]沈寻,丁华,武彩霞,等. 生姜有效部位对高脂血症大鼠的抗氧化作用研究[J].中国生化药物杂志, 2005,26(6): 330-332
[24]武彩霞,徐丽洒,魏欣冰,孙霞,丁华,程秀民.生姜有效部位对实验性高脂血症大鼠血清血栓素A2、前列环素和脂质过氧化的影响[J]. 中国药学杂志, 2006, 41(14): 1066 –1068
[25]WU CX, CHENG XM, WEI XB, et al. Influence of effective parts of Zingiber officinale on blood lipid and serum nitric oxide in experimental hyperlipidemia rats [J]. Chin Tridit Herb Drugs (中草药), 2006, 37(1): 92-94
[26]Tanabe M, Chen YD, Saito K, et al. Cholesterol biosynthesis inhibitory component from Zingiber officinale Roscoe [J].ChemPharmBull(Tokyo),1993,41(4):710-713
[27]丁志平.生姜油降血脂抗动脉粥样硬化的实验研究[D] .学位论文,1993
[28]王贵林,朱路.生姜油的抗炎作用[J].中药药理与临床,2006;22(5):26-28
[29]王贵林,朱路,张渊.生姜油对佐剂性关节炎小鼠关节液前列腺素E2的影响[J].长江大学学报(自科版),2006,3(4):245-248
[30]Sharma JN, Srivastava KC, Gan EK. Supp ressive effects of eugenol and ging- er oil on arthritic rats[J]. Pharmacology, 1994,49 (5):314-318
[31]Peng WH. Analgesic and antiinflammatory activities of ( 6 ) -gingerol[J]. Journal of Ethnopharmacology, 2005,96:207～210
[32]朱路,王贵林,姚观平,等.生姜油抗炎和抗超敏反应作用研究[J].时珍国医国药, 2007,18(1):105-106
[33]宋学英,白进发,王桥,等.生姜对小鼠缺氧耐受时间的影响[J].中国医药学报,1998,13(3):70-72
[34]吴智兵,汪栋材.生姜水提物和内毒素诱导脑缺血耐受的比较研究[J].中国中医药信息杂志,2006,13(7):29-30
[35]王军,张薇,王玉升,等.生姜水提物对脑缺血再灌注损伤的保护作用[J]. 中医药临床杂志,2007,19(1):23-24
[2] 王建枝.脑功能不全. 陈修,陈维洲,曾贵云主编.心血管药理学[M].第三版.北京:人民卫生出版社,2003.236-258
[3] 方舒东,朱也森.脑缺血再灌注损伤的病理生理研究进展[J].医学综述,2006 ,12 (18):1114-1116
[4] 刘启锋,刘明,刘玉河,等.脑缺血再灌注损伤机制研究进展.中华神经外科疾病研究杂志[J],2006;5(6):566-568
[5] 韩敏,刘艳凯,薛茜.脑缺血再灌注损伤的研究进展[J].河北北方学院学报(医学版), 2006,23(5):75-78
[6] Ritter LS, Orozco JA, Coull BM, et al. Leukocyte accumulation and hemodynamic changes in the cerebral microcirculation during early reperfusion after stroke [J]. Stroke, 2000, 31(5):1153-1162.
[7] Danton GH, Dietrich WD. Inflammatory mechanisms after ischemia and stroke [J]. Neuropathol Exp Neurol, 2003, 62(2):127-137.
[8] DelZoppo GJ, Becker KJ, Hallenbeck JM. Inflammation after stroke: is it harmful [J]. Arch Neurol, 2001, 58(4):669-673.
[9] Fyijins CJM, Kappelle LJ. Inflammatory cell adhesion molecules in ischemic cerebral vascular disease[J]. Stroke, 2002, 33 (8): 2115-2123.
[10] Zimmerman GA, McIntyre TM, Mehra M, et al. Endothelial cell-associated intercellular adhesion [J]. J Cell Bio, 1990, 110(2): 529-540
[11] 朱道银.细胞因子.沈关心,王明丽,朱道银,等.微生物与免疫学[M].第5版.北京:人民卫生出版社,2003.53-57
[12] 黄启福,王元勋,卫洪昌,等.病理学.北京:科学出版社.2004,4:37-45
[13] 张娜,朱晓磊,李澎涛,等.胆酸、栀子苷及配伍对大鼠缺血再灌注脑组织TNF-α、IL-1β和ICAM-1含量的影响[J].中国医药学报,2003,18(8):463。
[14] Zhai Q H, Fut rell N, Chen FJ, et al. Gene exp ression of IL-10 in relationship to TNF-alpha, IL-1beta and IL-2 in the rat brain following middle cerebral artery occlusion [J]. Neurol Sci, 1997, 152 (2):119 - 124.
[15] 杨养贤,延卫东,乔晋,等.黄芩苷对大鼠缺血再灌注脑组织TNF-α、IL-1β表达的影响[J]. 西安交通大学学报( 医学版),2005, 26(3):220-224
[16] Barone FC, Arvin B, White RF, et al. Tumor necrosis factor - α:A mediator of focal ischemic brain injury[J ] . Stroke, 1997, 28 (6):1233 - 1244.
[17] Nawashiro H, Martin D, Hallenbeck JM. Neuroprotective effects of TNF binding proteinin focal cerebral ischemia[J]. Brain Res, 1997, 778(2):265 - 271.
[18] Shohami E, Bass R, Wallach D, et al. Inhibition of tumor necrosis factor - α( TNF - α) activity in rat brain is associated with cerebro protection after closed head injury[J]. Cereb Blood Flow Metab, 1996, 16(3): 378 - 384
[19]曹晓岚,任丽,臧运华.溶栓颗粒对脑缺血再灌注大鼠的脑保护作用研究[J].中西医结合心脑血管病杂志,2005,3(1):37-38
[20]陈寿权,王万铁,王明山,等.醒脑静对家兔脑缺血再灌流时TNF、IL - 1β、IL - 6 水平及脑超微结构影响的实验研究[J].中国急救医,2000,20(11):637-639
[21] 卢传坚,欧明,王宁生. 姜的化学成分分析研究概述[J]. 中药新药与临床药理,2003,14(3):215-217.
[22] 何文珊,严玉霞.生姜的化学成分与生物活性研究概况[J].中药材,2001, 24(5):376-379.
[23] 药理教研组. 鲜姜注射液抗炎消肿及镇痛作用实验研究[J]. 泸州医学院学报,1979,(3):1-3
[24] 张竹心. 生姜油的抗炎作用研究[J]. 中草药,1989;20(12):545-546.
[25] Penna SC, Mederios MV, Aimbire FS, et al. Anti-inflammatory effect of the hydralcoholic extract of Zingiber officinle rhizomes on rat paw and skin edema[J]. Phytomedicine, 2003, 10(5): 381-385.
[26] 王梦,钱江美,苏简单. 干姜乙醇提取物解热镇痛及体外抑菌作用研究[J]. 中药新药与临床药理,2003,14(5):299-301
[27] Ojewole JA. Analgesic, antiinflammatory and hypoGlycaemic effects of ethanol extract of Zingiber officinale (roscoe) rhizomes (zingiberaceae) in mice and rats [J]. Phytother Res, 2006, 20(9):764-772.
[28] 末川守. 关于生姜药理学的研究(第Ⅳ报).(6)-姜醇对于花生四烯酸代谢的作用[J]. 国外医学中医中药分册,1987,9(3):160-162.
[29]Grzanna R,Phan P,Polotsky A, et al. Ginger extract inhibits beta-amyloid peptide-induced cytokine and chemokine expression in cultured THP-1 monocytes [J]. Altern Complement Med, 2004, 10(6):1009-1013.
[30]徐叔云,卞如濂,陈修. 药理实验方法学[M]. 第 3 版. 北京:人民卫生出版社. 2002. 1065-1066.
[31]蔡飞,李彩蓉,吴基良,闵清,等.茶黄素对大鼠脑缺血再灌注炎性细胞因子的影响[J]. 中药药理与临床 2007,23(1):23-24
[32]李建兵,张静,吴纯慧.肿瘤坏死因子TNF-α与缺血性脑血管病相关性研究进展[J].淮海医药, 2005, 23(1): 86 -87
[33]罗正曜.休克. 杨惠玲,潘景轩,吴伟康主编.高级病理生理学[M].第二版.北京:科学出版社. 2006,7:106-121-129。
[34] Lavin SD, Hofman FM, Zlokovic BV. Circulating antibodyagainst tumor necrosis factor - αprotects rat brain from reperfusion injury[J]. J Cereb Blood Flow Metab, 1998, 18 (1):52 - 58.
[35]Clemens JA, Stephenson DT, Smalsting EB, et al. Global ischemia activates nuclear factor - KB inforebrainneurons ofrats [J]. Stroke, 1997, 28 (5): 1073 - 1081.
[36]Siren Al, Liu Y, Feuerstein G, et al. Increased release of tumor necrosis factor - αinto the cerebrospinal fluid and peripheral circulation of aged rats [J]. Stroke, 1993, 24(6): 880 - 888.
[37] Elneihoum AM, Falke P, Axelsson l, et al. Leukocyte activiation detected by increased plasma levels of inflammatory mediators in patients with ischemic cerebro vascular diseases [J]. Stroke, 1996, 27 (10): 1734 - 1738.
[38]霍则军,张莉,钱瑞琴.针刺不同穴组对全脑缺血再灌注大鼠TNF-α、IL-6 、WBC和自由基的影响[J],针刺研究, 2003,28(2):94-98
[39]高晶,郭玉璞.缺血后脑组织损伤中的炎细胞作用[J].中华神经科杂志,1999,32: 303-305
[40]郭壮丽,裴海涛. 急性脑缺血再灌注大鼠脑内IL-1β蛋白表达及针刺对其抑制作用的研究[J]. 中华物理医学与康复杂志, 2005, 27 (1):16-19
[41]吕洋, 沈维高, 刘国辉.脑缺血再灌注损伤中IL-1β的作用及机制[J]. 北华大学学报(自然科学版), 2004,5(6): 524-528
[42]宋月英,齐刚,韩景田,等. 大鼠全脑缺血再灌注损伤后脑内白细胞介素-1β 变化及红景天苷的影响[J]. 中国临床康复,2006,10(11):30-32
[43]Pera J, Zawadzka M, Kaminska B, etal. Influence of chemical and ischemic preconditioning on cytokine expression after focal brain ischemia[J]. Neurosci Res, 2004, 78(1):132-40
[44]李静,明亮,黄茸茸,等.黄芪提取物对大鼠全脑缺血再灌注后IL-1β、TNF-α和IL-6表达的影响[J].安徽医科大学学报,2005,40(6):512-514
[45] Relton JK, Martin D, Thompson RC et al. Peripheral administration of Interleukin-1 receptor antagonist inhibits brain damage after focal cerebral ischemia in the rat[J] . Exp Neurol , 1996,138 (2) :206～13
[46] Beamer NB, Coull BM, Clark WM, et al.Interleukin-1 and interleukin-6 receptor antagonist in acute stroke[J]. Ann Neuro , 1995,37 (6) :800～5
[47] Hara H, Fink K, Moskowitz MA ,et al. Attenuation of transient focal cerebral ischemicinjury in transgenic mice expressing a mutant ICE inhibitory protein[J]. Cereb Blood Flow Metab , 1997, 17(4) :370～5
[48]韦登明,黄光照,张益鹄,等.雷公藤内酯醇抑制大鼠脑局灶性缺血再灌注后脑组织IL-1βmRNA 表达的研究[J].中国药理学通报, 2003, 19(11): 1277～1280
[49]史朗峰,任惠民,白蓉,等. 国产降纤酶对缺血/再灌注大鼠脑组织TNF-α及IL-1βmRNA 表达的影响[J]. 中风与神经疾病杂志,2004, l-1( 6):544-546
[50]王贵林,朱路.生姜油的抗炎作用[J].中药药理与临床,2006,22(5):26-28。
[51]季永侠,袁荣峰.VD大鼠血清及脑脊液中IL-6水平变化[J].中国实用医药杂志, 2007, 2 (9): 28-29
[52]崔丽,郑惠民.白细胞介素-6与缺血性脑损伤.国外医学脑血管疾病分册[J],2002,10(2):134-136
[53]雷秀珍,李秀英.异丙酚对大鼠局灶性脑缺血/再灌注损伤期炎性细胞因子的影响[J].重庆医科大学学报, 2004, 29(5): 622-625
[54]张泽舜. 细胞因子在脑外伤早期的变化和对人胚神经干细胞的影响[J].中华神经外科杂志, 2003,8(5): 233 -235
[55] Ahn MJ,Sherwood ER,Prough DS,et al.The effect of traumatic brain injury on cerebral blood flow and brain tissue nitric oxide levels and cytokine expression[J]. Neurotrauma, 2004, 21(10): 1431 - 1442.
[56]郭宗泽,冷汝溥,李光宇.IL-6和TNF-α在弥漫性脑损伤大鼠额叶的表达及血清中含量变化[J]. 解剖科学进展,2006,12(4):311～313
[57]向赟,鲁雄兵,许晓莉,等.大鼠脑缺血-再灌注后脑脊液及脑皮质海马TNF-α与IL-6的变化[J].中国康复,2004,19(3):139-141
[58]钟德桥,李少华,高观月,等.大鼠急性脑缺血及再灌注时白细胞介素(IL - 6、IL - 8) 的变化及意义[J]. 江汉大学学报(医学版), 2002,30(2):11-12
[59]王贵林,朱路,张渊.生姜油对佐剂性关节炎小鼠关节液前列腺素E2的影响[J].长江大学学报(自科版),2006,3(4):245- 248
[60]韩英,刘楠,陈荣华,等.白细胞介素8在大鼠脑缺血再灌注损伤中的变化[J].中国动脉硬化杂志,2005, 13(2): 155- 157
[61] Harada A , Sekido N , Akahoshi T, Wada T, Mukaida N , Matsushima K. Essential involvement of interleukin-8 (IL-8) in acute inflammation[J]. LeukoeBiod, 1994, 56 (5): 559-564
[62] Schroder JM, Mrowitz U, Christophers E. Purification and partial biologic char acterization of human lymphocyce-derived peptide with potent neutrophil-stimulating activity[J]. Immunol, 1988, 140(10): 534-540
[63]Kostulas N , Kividakk P, Huang Y, Matusevicius D, Kostulas V, Link H. Ischemic stroke is associated with a systemic increase of blood mononuclear cells expressing interleukin-8 mRNA [J]. Stroke, 1998, 29(2): 462-466
[64] Yamasaki Y, Matsuo Y, Matsuura N , Onodera H , Itoyama Y, Kogure K. Transient increase of cytokine-induced neutrophil chemoattactant , a member of the interleukin-8 family , in ischemic brain areas after focal ischemia in rat[J]. Stroke, 1995, 26 (2): 318-323
[65]刘士福.大鼠脑缺血再灌注损伤血清IL-8和中性粒细胞趋化指数的变化[J]. 放射免疫学杂志,2006,19(2):116-118
[66] Beech JS, Reckless J, Mosedale DE, et al. Neuroprotection in ischemia-reperfusion injury: an anti-inflammatory approach using a novel broad-spectrum chemokine inhibitor[J]. Cereb Blood Flow Metab, 2001, 21 (6): 683-689
[67]Aloisi F, Car F, Borsellino G. Production of hemolymphopoietic cytokines (IL-6, IL-8, colonystimulating factors) by normal human astrocytes in response to IL-1βand necrosis factor - α[J]. Immunol, 1992, 149: 2358
[68]Hofman FM, Chen P, Jeyaseelan R, et al. Endothelin-1 induces production of the neutrophil chemotactic factor interleukin-8 by human brain -derived endothelial cells[J]. Blood, 1998, 92: 3064
[69]刘继明,朱兴国,朱华亭,等. IL-8 单抗保护兔肝脏免受中性粒细胞介导的再灌注损伤[J].上海免疫学杂志,2001,21(1):7-10
[70]曹秋云,江开达,潘旭东.大鼠脑缺血再灌注损伤 IL-8 变化的研究[J]. 放射免疫学杂志, 2001, 14(2): 123-125
[71]徐霖,李树浓.移植免疫.杨惠玲,潘景轩,吴伟康主编.高级病理生理学[M]. 第二版.北京:科学出版社. 2006,21:378-380。
[72]尹静,杨,李俐涛,等.粘附分子与缺血性脑损伤. 脑与神经疾病杂志[J],2007,15(4):318-320
[73]Zhang RL, Zhang ZG, Chopp M, et al. Thrombolysis with tissue plasminogen activator alters adhesion molecule expression in the ischemic rat brain [J]. Stroke, 1999, 30:624～629
[74]陈邦国 ,毛庆菊 ,孔立红.电针对局灶性脑缺血再灌注大鼠缺血脑区细胞黏附分子表达的影响[J].湖北中医学院学报,2006,8(4):3-5
[75] Danton GH, Diet rich WD. Inflammatory mechanisms after ischemia and stroke [J]. Stroke,2003,62(2):127～137
[76] Zhang RL, Chopp M, Zologa C, et al. The temporal p rofiles of ICAM-1 protein and mRNA expression after transient MCA occlusion in the rat [J] . Brain Res, 1995, 682:182-188.
[77]郝延磊,蒲传强,朱克,张凤英,刘洁晓.细胞间粘附分子-1 和 E-选择素在局部脑缺血中的作用[J].中华老年心脑血管病杂志 2000, 2(1):54-57
[78]路文革,白宏英,王建平.垂体腺苷酸环化酶激活肽对大鼠脑缺血再灌注后细胞间黏附分子表达的影响[J]. 中国实用神经疾病杂志,2007,10(2):53-56
[79]苏红梅,朱广旗, 欧阳泠星,等.针刺对大鼠脑缺血再灌注损伤后ICAM-1表达的影响[J].上海针灸杂志,2006,25(12):30-32
[80]魏慧利,刘亚娴.地圣珠水煎剂对脑缺血再灌注后炎症反应的影响[J].中药药理与临床,2007,23(5):192-193
[81]向红兵,晋光荣 ,吴颢昕 ,等.大鼠多发性脑梗死后脑组织内皮细胞ICAM-1表达的变化[J].东南大学学报( 医学版)2004,23(5):295-298
[82]罗成基, 裴雪涛主编. 造血微环境[M].北京:世界医药出版社,2001:1
[83]司英健,张曦,陈幸华,等. VCAM-1 和EGFP双基因共表达重组腺病毒的构建[J]. 重庆医学,2007,36(17):1699-1703
[84]张丽慧,魏尔清.ICAM-1、VCAM-1在脑缺血损伤炎症机制中的作用及调控[J]. 中国药理学通报,2005, 21(11):1281～4
[85]雷万龙,袁群芳,张怀波,等.粘附分子表达和巨噬细胞浸润在缺血皮质和基底节区分布的比较[J].广东解剖学通报,1998,20(2):9-12
[86]Selakovic V, ColicM, JovanovicM et al. Cerebrospinal fluid and plasma concentration of soluble intercellular adhesion molecule 1,vascular cell adhesion molecule 1 and endothelial leukocyte adhesion molecule in patients with acute ischemic brain disease [J].Vojnosanit Pregl, 2003, 60(2):139 - 46.
[87]Montaner J, Rovira A, Molina CA et al. Plasmatic level of neuroin flammatory markers predict the extent of diffusion weighted image lesions in hyperacute stroke [J]. Cereb Blood Flow Metab, 2003, 23(12):1403 - 7.
[88]Zhang LH, Wei EQ. Neurop rotective effect of ONO21078, a leukotriene receptor antagonist, on transient global cerebral ischemia in rats [J]. Acta Pharmacol Sin, 2003, 24: 1241 - 7.
[89]Sander WE, Wilson RW, Ballantyne CM, et al. Molecular cloning and analysis of in vivo expression of P- selectin[J]. Blood 1992, 80(3):795
[90]才立云,赵宝春,耿旭. 血管黏附分子中选择素结构、功能及其表达的特点[J].中国组织工程研究与临床康复,2007,11(14): 2729-27312
[91]陈志鸿,宋宝辉,巴雪青.选择素及其配体的研究进展[J].国外医学遗传学分册, 2005, 28 (5):270-274
[92]刘玉珍,韩景献,蒋戈利,等.电针对大鼠脑缺血再灌注模型P-选择素和E-选择素表达影响的实验研究[J].时珍国医国药,2007,18(1):79-81
[93]Chen Y, Ruetzler C, Pandipati S, et al. Mucosal tolerance to E-selectin provides cell- mediated protection against ischemic brain injury[J]. Proc Natl Acad Sci USA, 2003, 100(25):15107
[94]刘皖生,唐乙,郑余银,等. 脑缺血再灌注的炎症损伤分子机制及吲哚美辛的保护作用[J].解放军医学杂志,2007,32(10):1051-1053
[95]盛文利,李锐,许晓伟,等. E- 选择素介导的大鼠脑缺血再灌注后继发炎症损伤的实验干预[J]. 中山大学学报(医学科学版),2007,28(1):48
[96]张国红,王春霖,吕平,等.大鼠局灶性脑缺血再灌注后不同时间E-选择素、P-选择素和细胞间粘附分子-1的表达[J].中国药理学通报,2005,21(10): 1218～23
[97]HUANG J, CHOUDHRI T F, WINFREE C J, et al. Postischemic cerebrovascular E - selectin expression mediates tissue injury in murine stroke [J]. Stroke, 2000, 31 (12): 3047- 3053.
[98]Suzuk H, Abe K, Tojo S, et al. Post ischemic expression of P-selectin immunoreactivity in rat brain [J].Neuosci Lett,1997,228:151-154
[99]阎俊,周红.选择素与缺血性脑血管病[J].国外医学脑血管疾病分册.2005,13 (11): 857- 859
[100]曾丽莉,刘振国.P-选择素与缺血性脑血管病[J].上海第二医科大学学报,2002,22 (2): 182-185
[101]倪红,储照虎,刘新亚.选择素与缺血性脑卒中[J].神经疾病与精神卫生, 2006, 6(3): 227-230
[102]Yao L, Setiadi H, Xia IJ, et al. Divergent inducible expression of P- selectin and E- selectin in mice and primates [J]. Blood, 1999 , 94(11) : 3820～3828
[103]Thomas WS , Mori E , Copeland BR , et al.Tissue factor contributes to microvascular defects after focal cerebral ischemia [J ] 1 Stroke,1993 , 24 : 847～853
[104]曾杨滨,杨期东,彭光莹,等.可溶性E-和P-选择素与急性脑梗死的关系[J].中国急救医学,2000,20(12):715-716
[105]姚巍,陈天铎,李培杰,等.可溶性P-选择素、血小板激活因子与急性脑梗死关系. 中国临床神经科学,2002,10(1):28-30
[106]狄政莉,万琪,王洪典,等.选择素在大鼠全脑缺血再灌注微血管内皮细胞损伤中的作用[J]. 中风与神经疾病杂志, 2002,19(4): 194 - 96
[1]罗集鹏,张保国,赵冰清,等.生药学[M].北京:中国医药科技出版社.1996,10:154-157
[2]匡芮,匡轩,朱海涛.生姜的功能因子与医疗保健作用[J].中国食物与营养, 2007, (2): 52-53
[3]何文珊,严玉霞,郭宝江.生姜的化学成分与生物活性研究概况[J]. 中药材,2001;24(5): 376-379.
[4]Ahmed RS, Seth V, Pasha ST, et al. Influence of dietary ginger(Zingiber officinales Rosc) on oxidative stress induced by malathion in rats[J]. Food Chem Toxical, 2000,38(5):443-450
[5]Ahmed RS, Seth V, Baneijee BD, et al. Influence of dietary ginger(Zingiber officinales Rosc) on antioxidant defense system in rat: comparison with ascorbic acid[J]. Indian J Exp Biol,2000,38(6):604-606
[6] 刘金玲,王卫东,郭景云. 鲜生姜提取液抗氧化作用的探讨[J]. 河南中医, 1996;16(3):156.
[7] 柳乃奎,黄雪松.姜酮、脱氢姜酮、姜酚肟对两种自由基的清除作用[J]. 食品科学,2004, 25(6):169-172
[8] 沈 寻,丁华,武彩霞,等. 生姜有效部位对高脂血症大鼠的抗氧化作用研究[J].中国生化药物杂志, 2005,26(6): 330-332
[9] 黄雪松,王建华,路福绥.生姜抗氧化作用的研究[J].食品工业科技,1997, 4:16-17
[10]Ippoushi K, Azuma K, Ito H, et al. 6-Gingerol inhibits nitric oxide synthesis in activated J774.1 mouse macrophages and prevents peroxynitrite-induced oxidation and nitration reactions[J]. Life Sci,2003;73(26):3427-37.
[11] 张关亭,王军,张磊,刘惠霞,张薇.生姜水提物对全脑缺血再灌注大鼠凝血功能的影响[J].中医研究,2007,20(4):18-20
[12] 李海平,刘宁,霍贵成.生姜的抗动脉粥样硬化作用[J].国外医学卫生学分册,2003, 30 (2): 98-101
[13]Srivastava KC. Isolation and effects of some ginger components on platelet aggregation and eicosanoid biosynthesis[J]. Prostaglandins Leukt Med, 1986; 25(2-3): 187-198.
[14]Srivas KC. Effects of aqueous extracts of onion, garlic and ginger on platelet aggregation and metabolism of arachidonic acid in the blood vascular system: in vitro study [J].Prostaglandins Leukot Med,1984, 13(2):227-235.
[15]Suekawa M, Yuasa K, Isono M, et al. Pharmacological studies on ginger. IV. Effect of (6)-shogaol on the arachidonic cascade [J].Nippon Yakurigaku Zasshi,1986,88(4): 263-269
[16]Srivastava KC. Effect of onion and ginger consumption on platelet thromboxane production in humans [J].Prostagl and Leukotr Essent Fatty Acids, 1989, 35(3):183-185.
[17]陈燕, 田泽, 葛毅强, 等. 姜油树脂调节血脂和抗血小板聚集作用的研究[J]. 营养学报[J],2001,23(4):338-341.
[18]高立波,姜波,李志坚,等. 姜总酮对血小板功能及血栓形成的影响[J]. 中国药理学通报[J],1996,12(3):288.
[19]Koo KL,Ammit AT,Tran VH,et al. Gingerols and related analogues inhibit arachidonic acid-induced human platelet serotonin release and aggregation [J].Thromb Res,2001,103(5):387-397.
[20]Nurtjahja-Tjendraputra E, Ammit AJ, Roufogalis BD, et al. Effectiveanti-platelet and COX-1 enzyme inhibitors from pungent constituents of ginger[J].Thromb Res,2003, 111(4-5):259-65.
[21]Kiuchi F, Shibuya M, Sankawa U. Inhibitors of prostaglandin biosynthesis from ginger [J]. Chem Pharm Bull (Tokyo),1982 Feb;30(2):754-757.
[22]卢传坚.姜对脑血管疾病的作用与途径[J].中国临床康复,2005,9(45):187 –189
[23]沈寻,丁华,武彩霞,等. 生姜有效部位对高脂血症大鼠的抗氧化作用研究[J].中国生化药物杂志, 2005,26(6): 330-332
[24]武彩霞,徐丽洒,魏欣冰,孙霞,丁华,程秀民.生姜有效部位对实验性高脂血症大鼠血清血栓素A2、前列环素和脂质过氧化的影响[J]. 中国药学杂志, 2006, 41(14): 1066 –1068
[25]WU CX, CHENG XM, WEI XB, et al. Influence of effective parts of Zingiber officinale on blood lipid and serum nitric oxide in experimental hyperlipidemia rats [J]. Chin Tridit Herb Drugs (中草药), 2006, 37(1): 92-94
[26]Tanabe M, Chen YD, Saito K, et al. Cholesterol biosynthesis inhibitory component from Zingiber officinale Roscoe [J].ChemPharmBull(Tokyo),1993,41(4):710-713
[27]丁志平.生姜油降血脂抗动脉粥样硬化的实验研究[D] .学位论文,1993
[28]王贵林,朱路.生姜油的抗炎作用[J].中药药理与临床,2006;22(5):26-28
[29]王贵林,朱路,张渊.生姜油对佐剂性关节炎小鼠关节液前列腺素E2的影响[J].长江大学学报(自科版),2006,3(4):245-248
[30]Sharma JN, Srivastava KC, Gan EK. Supp ressive effects of eugenol and ging- er oil on arthritic rats[J]. Pharmacology, 1994,49 (5):314-318
[31]Peng WH. Analgesic and antiinflammatory activities of ( 6 ) -gingerol[J]. Journal of Ethnopharmacology, 2005,96:207～210
[32]朱路,王贵林,姚观平,等.生姜油抗炎和抗超敏反应作用研究[J].时珍国医国药, 2007,18(1):105-106
[33]宋学英,白进发,王桥,等.生姜对小鼠缺氧耐受时间的影响[J].中国医药学报,1998,13(3):70-72
[34]吴智兵,汪栋材.生姜水提物和内毒素诱导脑缺血耐受的比较研究[J].中国中医药信息杂志,2006,13(7):29-30
[35]王军,张薇,王玉升,等.生姜水提物对脑缺血再灌注损伤的保护作用[J]. 中医药临床杂志,2007,19(1):23-24