摘要
目的:观察补肾活血对大鼠慢性高眼压(elevated intraocular pressure, EIOP)模型初级视皮质损害的干预作用,并对其作用机理进行初步探讨。
方法:采用烙闭上巩膜静脉法,烙闭大鼠3支上巩膜静脉,制作大鼠慢性EIOP模型,随机分为3组:模型组,给药组,空白组。连续灌胃8周,并于8周末处死大鼠,观察其对EIOP大鼠眼压(intraocular pressure, IOP),初级视皮质(primary visual cortex, PVC)尼氏小体(nissl bodies)含量、神经元细胞超微结构及脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)表达的影响。
结果:本实验采用的烙闭上巩膜静脉的造模方法使大鼠IOP明显升高,在造模后8周(实验结束)IOP仍为造模前的大约3倍;补肾活血对大鼠慢性EIOP模型初级视皮质损害的干预作用如下:轻度降低IOP,提高初级视皮质尼氏小体(nissl bodies)含量、增强脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)的表达及改善神经元细胞超微结构。
结论:采用烙闭上巩膜静脉的方法建立的慢性EIOP大鼠模型操作简单、效果稳定,可获得不少于两个月的中等度EIOP;补肾活血可以有效的保护慢性EIOP大鼠模型视功能,其作用主要表现在轻度降低IOP,提高初级视皮质尼氏小体含量、增强BDNF表达、改善神经元细胞超微结构。
Objective:To observe the effect traditional Chinese medicine(TCM) of BuShenHuoXue on primary visual cortex damage in rat model of chronic, elevated intraocular pressure, and explore the mechanism of it initially.
Methods:By unilaterally cauterizing 3 episcleral vessels,the rat model of chronic, moderately elevated intraocular pressure was gotten.30 rats were divided into 3 groups randomly:model group, treatment group,and normal control group.After given drugs or normal saline for 8 weeks,the rats were put to death. To observe the effection by intraocular pressure(IOP), content of nissl bodies,expression of brain-derived neurotrophic factor,ultrastructure of neuron cell in the primary visual cortex (PVC)
Results:Chronic elevated intraocular pressre was gotten and there was an approximately 3-fold increase in IOP for 2 months comparing to pre-models building. The Intervention:BuShenHuoXue of TCM can lower IOP slightly, and increase content of nissl bodies,enhance expression of brain-derived neurotrophic factor, and improve ultrastructure of neuron cell.
Conclusion: This model is simple and stable, and moderately elevated IOP can last at least 2 months. BuShenHuoXue of TCM can protect the visual function from elevating intraocular pressre,by increasing content of nissl bodies,enhancing expression of brain-derived neurotrophic factor,improving ultrastructure of neuron cell.
引文
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