四列藻和东海原甲藻的光周期效应研究
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摘要
以四列藻(Tetraselmis tetrathele)和东海原甲藻(Prorocentrumdonghaiense Lu)为试验材料,对其光周期效应进行了探讨和研究,期望探索此两种海洋单细胞微藻光周期效应的有无和不同种类的微藻的光周期效应的异同。设置了短光周期(A)和长光周期(B)两组试验:A组分光周期为1h、2h、4h、6h、8h、10h和12h 7个处理,B组分光周期为12h、14h、16h、18h、20h、22h和23h 7个处理,测量了细胞密度、叶绿素-a、蛋白质,可溶性糖含量和生物量等指标。结果表明:(1)四列藻和东海原甲藻都具有显著的光周期效应,但这两种微藻的光周期特征具有明显差异。四列藻具有一对个光周期的适应范围,细胞生长对应光周期变化呈近似正态分布;而东海原甲藻的细胞生长和光周期变化呈现正相关的关系。(2)四列藻和东海原甲藻的光周期适宜范围均较宽,在光周期为6h-18h范围内,四列藻的细胞密度、叶绿素a含量和生物量等指标均呈现较高水平;东海原甲藻在光周期8h以上时,呈现出光周期越长生长越好的特征。(3)东海原甲藻和四列藻均对不同的光周期处理均表现出不适、亚适和最适的三个区间:东海原甲藻在6h以下为不适区间,8h-18h为亚适区间,20h以上为最适区间;四列藻的最适范围为8h-18h,光周期小于4h和大于18h为其不适区间,光周期6h-8h为亚适区间。(4)对于经济微藻四列藻而言,规模化培养中,每天满足10h左右的光照时间,即可取得较高的生物量;对于东海原甲藻,如果当地处于强光照和长光周期条件的海域,应当注意预防其赤潮的发生。研究结果对于经济微藻的规模化培养和有害藻华的防治有一定参考价值。
In this paper, the marine micro-algae Tetraselmis tetrathele and Prorocentrum donghaiense were used as experimental material in order to study the photoperiod effect of them. We hope to find whether they have the Photoperiodic response and we want to know the difference of Photoperiodic response between these two kinds of algae if they have. There were 2 man-made groups (A and B) experiments by the length of different photoperiod. Group A included 7 treatments of photoperiod designed not longer than 12h (1h, 2h, 4h, 6h, 8h, 10h and 12h). Group B also included 7 treatments of photoperiod designed not less than 12h (12h, 14h, 16h, 18h, 20h, 22h and 23h). Cells density, biomass, content of chlorophyll-a, intracellular protein and dissolved carbohydrate of all these two kinds of algae were determined. The result showed: (1) T. tetrathele and P. donghaiense all have obvious Photoperiodic effect. There are obvious differences between these two kinds of algae. The growth curve of T. tetrathele presented approximate normal contribution corresponding to the change of photoperiod and there was positive relationship between the growth of P. donghaiense and the change of photoperiod. (2) The adaptive range of these two kinds of algae are quite broad. The cells' index of T. tetrathele, such as cells density, biomass and content of chlorophyll-a, all are high in the Photoperiodic range of 6h-18h. P. donghaiense grows better as the photoperiod prolongs longer. (3) The growth of two kinds of algae all presented optimum range, suboptimum range and unsuitable range of photoperiod. P. donghaiense's suboptimum photoperiod range is 8h-18h, algae's unsuitable photoperiod range is shorter than 6h and optimum photoperiod rangeis longer than 20h. The photoperiod is T. tetrathele's unsuitable range when is shorter than 4h or longer than 20h. T. tetrathele's optimum photoperiod range is 8h-18h and suboptimum photoperiod range is 6h-8h. (4) As to I tetrathele, it is appropriate to use the photoperiod of about 10h in massive production. In the area of marine or sea, which it's long day or sunny, it ought to avoid the break out of P. donghaiense harmful algal blooms.
引文
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