苍术、白术中有效成分研究及中药材元素指纹图谱的建立
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摘要
中药是中华民族智慧的璀璨结晶,而有效成份是防治疾病的物质基础,是药效、毒理及代谢的研究的基础,对最终阐明中草药治疗疾病机理、安全性评价和产品质量控制有重要意义。
有效性、安全性和稳定性是任何药物制剂最基本的要求。在中国药典中有20%的方剂采用煎煮方式提取有效成分,在加热过程中肯定会发生一些化学反应,这些化学反应的产物可能是有着可靠疗效的成分,也可能是毒性成分。因此对药物煎煮过程中成分的变化进行有效地监控,是保证有效和安全的重要条件。
任何物质都是以元素为基础的。药材的药效及道地性都能从元素特征反映出来。建立中药材的元素指纹图谱,可以为中药材真伪鉴别、产地及道地性的判断提供可靠依据。
论文主要的研究内容及所得结果如下:
1.对南北苍术在煎煮过程中药材的化学成分的变化进行了深入的研究。运用制备色谱对煎煮过程中新生成物质进行分离纯化和结构鉴定,发现北苍术经过水煎煮后产生了3,5-二羟基-2-甲基-4H-吡喃-4酮和5-羟甲基糠醛两个物质,而道地药材茅苍术却没有此现象。这可能是茅苍术体现道地性的一个方面。
2.优化了白术中抗炎,抗癌的有效成分白术内酯Ⅲ分离纯化方法。优化后的方法得到的产品纯度高达97.8%,该方法节省溶剂,萃取所用的溶剂可以回收,对环境无污染,而且不像柱层析分离方法对人力物力消耗都非常大,适合于工业化生产。
3.应用ICP-MS和AAS法分析赤芍,白芍,大黄,河北苍术,内蒙古苍术,黄芩,丹参这些药材中的47种微量、痕量、稀土金属及常量元素,并从而构建了这些药材的元素指纹特征,为中药道地性及药效特征研究提供有效的依据。
Traditional Chinese medicine is the gem of China. The effective composition is the most important material basis of efficacy, toxicology and metabolism. And which has important significance in finally elucidating the mechanism of TCM therapy, in controlling product quality and in the evaluation of safety.
Validity, security and stability are the most fundamental requirements of any pharmaceutical preparation. Almost one fifth of Chinese traditional medicines are decocted by water. However, the compositions and contents of the chemical components may be changed in the process of decocting. These reaction products may be the curative effect compositions, but they may also be the toxic components. Therefore, effectively monitoring the changes of components is the key points to ensure the validity, security and stability of herb drugs.
Element is the basis of everything. The element fingerprint can reflect the drug efficacy and the genuineness in some extent. The element fingerprint of herb which will be provided reliable basis for the identification of false and true herb, the identification of producing area and the judgment of the genuineness.
The main contents and results of this paper include:
1. In the first section. We have made some researches on the chemical components changes of Atractylodes lancea and Atractylodes chinensis' Water-extract in the process of decocting, and separation and purification of the new compounds in the Water-extract. The 3, 5-dihydroxy-2-methyl-4H-Pyran-4-one and 5-(hydroxymethyl)-2-Furancarboxaldehyde were found in the Water-extract of Atractylodes chinensis but weren't found in the Water-extract of genuine medicinal materials Atractylodes lancea. This may be an embodiment of genuineness.
2. We optimized the method of separation and purification of atractylenolide III which is anti-inflammation and anticancer compound. The purity of atractylenolide III was as high as 97.8%. However, this new method has the merits of low consume, low pollution and save manpower. So it can provide more suitable method for the industrial production of atractylenolide III.
3. Aim to establish the element fingerprint of these herbs. We determination the content of elements in Radix Paeoniae Rubra、Paeonia lactiflora Rhubarb、Heibei Atractylodis lancea、Neimenggu Atractylodis lancea、Scutellaria baicalensis Georgi、Salvia Miltiorrhiza by ICP-MS and AAS. For provided reliable basis of the TCM genuineness and the producing area.
有效性、安全性和稳定性是任何药物制剂最基本的要求。在中国药典中有20%的方剂采用煎煮方式提取有效成分,在加热过程中肯定会发生一些化学反应,这些化学反应的产物可能是有着可靠疗效的成分,也可能是毒性成分。因此对药物煎煮过程中成分的变化进行有效地监控,是保证有效和安全的重要条件。
任何物质都是以元素为基础的。药材的药效及道地性都能从元素特征反映出来。建立中药材的元素指纹图谱,可以为中药材真伪鉴别、产地及道地性的判断提供可靠依据。
论文主要的研究内容及所得结果如下:
1.对南北苍术在煎煮过程中药材的化学成分的变化进行了深入的研究。运用制备色谱对煎煮过程中新生成物质进行分离纯化和结构鉴定,发现北苍术经过水煎煮后产生了3,5-二羟基-2-甲基-4H-吡喃-4酮和5-羟甲基糠醛两个物质,而道地药材茅苍术却没有此现象。这可能是茅苍术体现道地性的一个方面。
2.优化了白术中抗炎,抗癌的有效成分白术内酯Ⅲ分离纯化方法。优化后的方法得到的产品纯度高达97.8%,该方法节省溶剂,萃取所用的溶剂可以回收,对环境无污染,而且不像柱层析分离方法对人力物力消耗都非常大,适合于工业化生产。
3.应用ICP-MS和AAS法分析赤芍,白芍,大黄,河北苍术,内蒙古苍术,黄芩,丹参这些药材中的47种微量、痕量、稀土金属及常量元素,并从而构建了这些药材的元素指纹特征,为中药道地性及药效特征研究提供有效的依据。
Traditional Chinese medicine is the gem of China. The effective composition is the most important material basis of efficacy, toxicology and metabolism. And which has important significance in finally elucidating the mechanism of TCM therapy, in controlling product quality and in the evaluation of safety.
Validity, security and stability are the most fundamental requirements of any pharmaceutical preparation. Almost one fifth of Chinese traditional medicines are decocted by water. However, the compositions and contents of the chemical components may be changed in the process of decocting. These reaction products may be the curative effect compositions, but they may also be the toxic components. Therefore, effectively monitoring the changes of components is the key points to ensure the validity, security and stability of herb drugs.
Element is the basis of everything. The element fingerprint can reflect the drug efficacy and the genuineness in some extent. The element fingerprint of herb which will be provided reliable basis for the identification of false and true herb, the identification of producing area and the judgment of the genuineness.
The main contents and results of this paper include:
1. In the first section. We have made some researches on the chemical components changes of Atractylodes lancea and Atractylodes chinensis' Water-extract in the process of decocting, and separation and purification of the new compounds in the Water-extract. The 3, 5-dihydroxy-2-methyl-4H-Pyran-4-one and 5-(hydroxymethyl)-2-Furancarboxaldehyde were found in the Water-extract of Atractylodes chinensis but weren't found in the Water-extract of genuine medicinal materials Atractylodes lancea. This may be an embodiment of genuineness.
2. We optimized the method of separation and purification of atractylenolide III which is anti-inflammation and anticancer compound. The purity of atractylenolide III was as high as 97.8%. However, this new method has the merits of low consume, low pollution and save manpower. So it can provide more suitable method for the industrial production of atractylenolide III.
3. Aim to establish the element fingerprint of these herbs. We determination the content of elements in Radix Paeoniae Rubra、Paeonia lactiflora Rhubarb、Heibei Atractylodis lancea、Neimenggu Atractylodis lancea、Scutellaria baicalensis Georgi、Salvia Miltiorrhiza by ICP-MS and AAS. For provided reliable basis of the TCM genuineness and the producing area.
引文
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