不同质地土壤玄参生长发育及质量研究
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摘要
药用植物玄参为常见中药材,为根茎入药,生育主要分布浙江、安徽、江苏、福建、江西、湖南、贵州、陕西、重庆等地。近年来,随着中药产业化、现代化推进,玄参入药量日益增加,栽培面积日趋扩大,导致玄参的生境出现差异,从而影响其生长发育和有效成分的累积,进而导致入药质量差异。目前玄参人工种植技术方面的研究较少,针对土壤因子对玄参生长发育和有效成分含量影响的系统性研究更少。
     本论文从栽培角度,选取影响玄参生长发育和成药的关键因子-不同质地土壤为入题点,配合栽培试验大田试验,农家管理,阐述了土壤质地对玄参生长过程中形态指标、生理指标、干物质积累和分配、营养物质吸收积累与动态转移,以及有效成分含量和玄参产量的影响,优选了玄参生长适宜的土壤质地,深入探讨了玄参的土壤适宜性,为不同质地土壤生境下玄参的质量控制与农业管理提供了建议,为GAP栽培与产业化发展奠定了基础。
     本论文主要研究的内容与目的:
     1.立足西南地域中、结合中高海拔长期玄参的栽培历史,先后通过一线栽培人员、科技特派员等对质地进行选取;结合实验室条件酸碱度、水分、容重、机械颗粒性质、质地、有机质、土壤全量的氮、磷、钾、钙、镁以及有效氮、磷、钾、钙、镁;土壤全量的铜、锌、铁、锰和有效铜、锌、铁、锰等指标的测定结果确定了西南高山地域主要用于栽培的3类土壤,5类生境质地,并依此依据布置大田试验。
     2.对不同质地生境下玄参的各生育期的株高、主茎基粗、主茎鲜干重、一级分茎数目、鲜干重、主茎叶数目及鲜干重,分茎叶的数目及鲜干重,稳定叶的数目及鲜干重,叶面积及叶绿素含量,块根的数目、长粗及鲜干重、子芽的数目及鲜干重、芦头的长及鲜干重、须根的长及鲜干重等指标进行获取(通过定株测量兼定期采样方式),并对上述植株各个器官不同生长期的氮、磷、钾、钙、镁、铜、锌、铁、锰等营养元素的含量进行检测,旨以全面评定玄参的生长发育、质地土壤的适应性、最佳采收、最佳产量获取以及质量的保证提供坚定依据和保障。
     3.对不同质地土壤生长发育的玄参块根,进行水分、灰分、浸出物及有效成分(哈巴俄苷、麦角甾苷、安格洛苷C、肉桂酸)含量检测,并结合不同质地土壤玄参的适宜性对其质量优劣进行评定,以此评定和指导玄参的高产栽培实践合GAP发展。
     本论文研究主要结论:
     通过对玄参不同质地土壤生长发育的适应性研究,确定了石灰岩类重壤酸性(PH:5.29)砂粉质土壤为最宜玄参高山生长土质类型,石灰岩类砂粉质、黄土中壤粉砂质为较宜适应类型。
     通过对不同质地玄参生长发育形态学、栽培学研究,界定了高山玄参生长发育的主要生育期,了解了干物质积累动态变化、养分的动态吸收累积等规律,体现了3月~5月28日为苗期,株高特征指数为19.9cm,6月1日~8月15日为分枝拔节初期(6月出现分枝),株高特征指数为19.9~88.10cm;8月16月~8月28日为分枝盛期,株高特征指数为88.10~118.54cm;8月28月~9月14日为分枝后期,(子芽出现)株高特征指数为118.54~128.17cm;9月15日~9月28日为花枝出蕾期(花枝出现),株高特征指数为128.17~133.81cm;9月28日~11月为回苗采收期,植高特征指数为138.81~140.17cm等具体生长发育特点。
     得出了玄参高山栽培以2~3月播种为宜,此期以有机肥、氮磷钾肥、钙镁钾铜锌铁等肥投入为主,增加对低温调节力度度、肥水的管理力度;3~5月加强对杂草的田间管理,6~8月增加对氮钾肥的投入、增进杂草的管理力度,特别强调对纹枯病、黑斑病等病害的防治;9~10月加强对磷、钾肥的施用,控制氮的后期用量,加强回苗枯萎期的采收与营养生长调控,确保玄参成药产量与质量最优化,提供可行的农艺措施。
     由此,在玄参生长发育质地的差异背景下,获得了玄参生育特点的特征性,掌握了玄参生长发育的时效性,确定了成药经济部位形成的最优性,共同构成了玄参栽培条件下玄参生药发育与质量的优化模式,对不同质地玄参生长发育与栽培、质量控制、引种驯化提供了思路与参考。
The Chinese medicinal plant-Radix Scrophulariae,mainly distributes in Zhejiang,Anhui, Jiangsu,Fujian,Jiangxi,Hunan,Guizhou,Shanxi,Chongqing such as places.In recent years,along with the industrial production and modernized advancement of the traditional Chinese medicine, Radix Scrophulariae,as the dried root of Scrophularia ningpoensis Hemsl.,its demand increases day by day with its cultivation territory and the cultivated area.
     Following the growth,development dissimilarity of Radix Scrophulariae on different cultivation territory,its corresponding quality,effective component content and accumulation exist biggish difference.The research in this paper,we took the medicinal plant-Radix Serophulariae (Scrophularia ningpocnsis Hemsl.) as the object of study,used the cultivation experiment,selected the different experimental field soil,adopted the lama management of large field,carried on the synchronized monitor of its development under different soil condition,and executed the contrastive analysis of its growth,development,physiological characteristic,medication spot,effective component content,as well as the accumulation situation and so on,in order to provide more systematical theory basis and data support for AP cultivation and the quality rating of Radix Scrophulariae under the different soil condition.
     In this paper,the main content and purpose of the study are as follows:
     1.According to the geographical environment of experimental area and the cultivation history of Radix Scrophulariae,we selected nine experimental fields for the cultivation condition.The first choice of selection method was to adopt the cultivation staff and the local science and technology technical personnel carrying on the macroscopic preliminary designation;Once more,to sample the soil in different time and carry on the determination of its potential of hydrogen,the moisture content,the bulk specific gravity,mechanical pellet elements and nature,quality of material,organic matter,nitrogen,phosphorus,potassium as well as copper,zinc,iron,manganese such as elements, combining with its determination target,growth target,development situation in different time as well as target conditions of its major and trace elements,carrying on the classification and determination of the electoral district experimental field,we fixed on the type of cultivation experiment land finally.
     2.To sample and handle Radix Scrophulariae in each growth periods regularly,latter monitor the nitrogen,the phosphorus,the potassium such as major element of each growth spot, simultaneously exam copper,the zinc,the iron,the manganese and such as trace element in various soils,times and material spots,carry on the comparative analysis to the determination target,we have determined the best growth type for Radix Scrophulariae in various experimental fields and obtained the quite comprehensive knowledge and understanding of its growth and development.
     3.Through examining the root of Radix Scrophulariae in different soil in different times,we carried on the general quality detection and the appraisal to Radix Scrophulariae separately, including the moisture content,the ash,the extractives and HPLC content contrastive analysis of the effect ingredient.We carried on the comprehensive nature appraisal of its growth and development in different soil to the determination result,by this instructing cultivation and practice. In this paper,the main conclusion of the study is as follows:
     1.Through the sample determination of the southwest region mountain cultivation soil,we know that the southwest region Chongqing section,the Radix Scrophulariae cultivation soil type divides into three kinds,two great particle sizes,five big quality of material soil category,namely limestone,yellow soil,purple ochre three kinds;loam,heavy loam two kind of particle size types and emery powder nature,powder nature,powder sandy,granulated substance mucilage,sandy five idioplasms.This soil is meta-acid,the acidity(pH 4.55~5.29) in overall performance;The moisture content performance is the high elevation region watery(localization value 18.81%~22.32%);it displays the experimental field high curing degree in the soil bulk specific gravity(localization value 0.05~49.07 g·cm~(-3)).The soil which includes many organic matters(1.76~3.02%) and good structure is suitable for the growth and cultivation of Radix Scrophularia.
     2.To monitor the sample of Radix Scrophulariae regularly,the synthesis regular examination of its own element as well as other chlorophyll content,the adult plant target,we took the superiority land parcel which favor the growth of Radix Scrophularia as representatives.We confirmed the effective calcium content,the soil maturation,pH to be highest,high nitrogen,potassium, magnesium,calcium,high moisture content,high organic archery target,the heavy loam emery powder nature limestone class soil which is suitable for the growth and cultivation of Radix Scrophulariae.On the contrary regarding,high manganese,low pH value,heavy loam powder nature class soil,although not too favoring the growth and cultivation of Radix Scrophulariae as a whole,but we may consider the new research and innovation to the soil being suitable for Radix Scrophulariae under the cultivation measure and the agronomy method.
     3.To carry on the quality determination to the medicine root of Radix Scrophulariae in different soil and month,its result satisfied the quality and soil relevant variance analysis,and the fit and unfit quality sufficiency conformed to five broad heading soil difference attribute,namely:the first kind is a limestone class,heavy loam emery powder nature,high nitrogen,potassium,magnesium,calcium, high moisture content,high organic matter,high curing soil;Simultaneously it surpassed the powder sandy type,loam high phosphorus,low potassium,low calcium of the fourth kind;Next,it surpassed high manganese,low pH value and heavy loam of the second kind;Once more,it surpassed heavy soil granulated substance class,yellow soil high potassium,copper,low nitrogen,phosphorus, manganese content,the organic archery target of third-order;In finally it surpassed the loam sandy class,purple ochre high zinc,low calcium,low organic archery target of the fifth kind.It has manifested the quality suitability soil selectivity of Radix Scrophulariae,and conformed to original intention and the goal of the experiment.
     From this,it has provided the development selectivity,growth effectiveness,prepared medicine spot relevance for Radix Scrophulariae which constituted its cultivation growth pattern together.It also provided the mentality and the reference for the ecology and the quality research of Radix Scrophulariae.
引文
[1]高学敏,张德芹,张建军.实用本草纲目彩色图鉴[M].第一卷.北京:外文出版社,2006:222
    [2]周浓,白志川.玄参的质量评价及其指纹图谱研究[D].西南大学,2008
    [3]肖培根.中药志[M].第一卷北京:化学工业出版社,2002:366
    [4]方阵,王康才,等.促进玄参高产栽培技术[J].中药材,2000,23(11):669-671
    [5]王珲,陈平等.玄参总色素提取物抗炎镇痛活性的研究.中国医院药学杂志[J],2008,17:1456-1459
    [6]曾华武,李医明,贺祥,等.玄参提取物的抗炎和抗氧化活性[J].第二军医大学学报,1999,20(9):614-616.
    [7]倪正,蔡雪珠,黄一平,等.玄参提取物对大鼠血液流变性、凝固性和纤溶活性的影响[J].中国微循环,2004,8(3):152-153.
    [8]Erdos R F,et aL.Planta Med[J],1978,34:97
    [9]Protective effect of Scrophularia ningpoensis extracts on cerebral ischemia injury in rats.Huang Qian.Gong Qin-yan,et all.Chin J New Drugs Clin Rem[J],2004,6(23):323-327
    [10]孙奎,姜华.玄参中苯丙素苷对肝细胞损伤保护作用的研究[J].药学实践杂志,2002,20(4).:234-235
    [11]谢丽华,刘宏字,钱瑞琴,等.哈帕苷和哈帕酯苷对阴虚.小鼠免疫功能及血浆环化核苷酸的影响[J].北京大学学报医学版,2001,33(3):283-284.
    [12]毛小平,陈彩琼,毛晓健,等.玄参与黄芪配伍的实验研究[J].云南中医学院学报,1997,20(2):1-6.
    [13]李医明,蒋山好,等.玄参属植物化学成分与药理活性研究进展[J].中草药,1999,30(4):307-310.
    [14]袭维桂,等.玄参对心血管系统药理作用的研究[J].浙江医学,1981,3(1):11-13
    [15]李广粹,等.中国医学科学院论文报告会论文摘要[R].Ⅱ,1956:70
    [16]方阵,王康才,等.促进玄参高产栽培技术[J].中药材,2000,23(11):669-671
    [17]陈尚国,张世林,熊良恒,等.玄参规范化种植技术[J].中国农村科技,2006,13(10):9-10.
    [18]邹宗成,向开栋.底肥种类与栽培方式对玄参产量的影响[J].湖北农业科学,2004,43(5):75-77.
    [19]沈笑天,范艺宽.南阳烟区非根际土壤碳素与生态指标关系的分析[J].中国烟草科学,2008,29(6):54-58
    [20]余世金,吴唤玲.茯苓栽培场土壤生态变化的初步研究[J].安徽农学.2008,33(36):14662-14664
    [21]张向东,张晓虎.商洛南五味子种植区土壤肥力特征[J].西北农业学报,2008,6(17):329-333
    [22]郭巧生.药用植物栽培学[M]:北京高等教育出版社,2004
    [23]吴友根,郭巧生.广蕾香种植土壤和药材中有机氯农药及重金属残留分析[J].中国中药杂志,2008,13(33):1528-1532
    [24]任一猛,王秀全.农田栽参土壤的改良与培肥研究[J].吉林农业大学报,2008,2(30):176-179
    [25]蒋小军.生草栽培对柿产量品质和土壤理化性状的影响[J],中国果树,2008,3:32-35
    [26]冯晓丽,梁宗锁.不同土壤水分对玄参生长发育及有效成分积累的影响[D].西北农林科技大学,2008
    [27]黄晓燕,孙文基.玄参地上部分化学成分及生物活性研究[D].西北大学,2008
    [28]周浓,白志川.玄参HPLC指纹图谱的建立[J].湖北农业科学,2006,45(6):782-785
    [29]Kitagawa I et al.On the iridoid constituent from the roots of Scrophularia buergerana Miq.Chem Ararm Bull,1967,15(8)1254
    [30]Qian J,Hunkler D,RimpIer H Iridoid- rdated aglycone and its glycosides from Scrophularia ningpoensis Phytochemistry,1992,31:905
    [31]Fernande M A.Gareia M D.Saenz M Z Antibaeterial activity of the phenoic acids fractions of Scrophularla frutescens and Scrophularia sambucifoha[J]Ethnophatnaeo]1996 53(1):11
    [32]何林.武隆中药材产业发展迅速[J].农家科技.2006,1:26-26
    [33]张宪政,苏正淑.作物水分亏缺伤害生理研究概况[J].沈阳农业大学学报,1997,27(1):85-91
    [34]陈尚国,张世林.玄参规范化种植技术[J].中国农村科技,2006,10:9-10
    [35]孔德生,顾士领.玄参种植,加工,留种技术[J].安徽农业科学,2004,32(1):122-125
    [36]邹宗成,向开栋.底肥种类与栽培方式对玄参产量的影响[J].湖北农学,2004,05:75-76
    [37]韩学俭.玄参病虫害及其防治[J].科学种养,2007.1:29-29
    [38]陈道明,杨家春.玄参斑枯病发生特点及防治对策.中国植保导刊,2005,25(11):27-27
    [39]吴云,玄参的主要病虫害及防治技术[J].植物医生,2006,6(19):30-32
    [40]薛媛菲,梁宗锁.玄参生物学特性和规范化研究技术研究[D].西北农林科技大学,2008
    [41]纪薇,梁宗锁.玄参配方施肥规律研究[D].西北农林科技大学,2008
    [42]方阵,王康才等.促进玄参高产栽培技术[J].中药材,2000,23(11):669-671
    [43]高学敏.中药学[M].北京:中国中医药出版社,2002:168.
    [44]孙小花,沈光明,等.细穗玄参的化学成分研究-Ⅱ[J].西北植物学报,2006,26(2):412-415
    [45]Fernande M A.Gareia M D.Saenz M Z Antibaeterial activity of the phenoic acids fractions of Scrophularla frutescens and Scrophularia sambucifoha[J]Ethnophatnaeo]1996 53(1):11
    [46]李静.中药玄参环烯醚萜甘类成分分析的研究.西北大学硕士学位论文,2006.06
    [47]李医明,蒋山好,高文运,等.玄参的脂溶性化学成分[J].药学学报,1999,34(6):448.
    [48]白志川.不同产地与加工方法玄参中肉桂酸的含量测定.[J]西南农业大学学报(自然科学版),2006,26(3):425-427
    [49]王建华,谢丽华,刘烘宇,等.玄参不同加工品中哈巴俄苷与肉桂酸的HPLC含量测定[J].中国药学杂志,2000,35(6):375-378.
    [50]张洪霞,宋金玉,赵怀清,等.RP-HPLC法同时测定定玄参中肉桂酸、哈巴酯苷的含量[J].沈阳药科大学学报,2006,23(8):507-509,528
    [51]张道锦,王瑞,毕开顺.MECC法测定玄参中哈帕酯苷和安格洛苷C的含量[J].沈阳药科大学学报,2001,18(3):189-191
    [52]李静,赵勇,孙文.RP-HPLC制备色谱法分离玄参中的桃叶珊瑚苷和哈帕苷[J].中药 材,2006,29(11):1189-1190
    [53]Kitagawa I,Nishimura T,Takei M,et al.On the iridoid constituent isolated from the roots of Scrophularia buergerana Miq.[J].Chem pharm Bull,1967,15(8):1254-1256.
    [54]刘金平.北玄参根中4个具神经保护作用的新环烯醚萜苷[J].国外医药.植物药分册,2004,19(2):70-70
    [55]曾华武,李医明,贺祥等.玄参提取物的抗炎和抗氧活性[J].第二军医大学学报,1999,20(9):614-616
    [56]李医明,蒋山好,朱大元,等.玄参中微量单萜和二萜成分[J].解放军药学报,2000,16(1):22-24.
    [57]李医明,蒋山好,高文远,等.玄参中的苯丙素苷成分[J].中草药,1999,30(7):487-489.
    [58]丁岩芝,陈桐,卢肖永,等.ICP-AES测定野玄参中微量元素[J].贵州工业大学学报(自然科学版),2005,34(4):28-30.
    [59]李医明,蒋山好,高文远,等.玄参的脂溶性化学成分[J].药学学报,1999,34(6):448-450.
    [60]田庚元,邓军娥.玄参多糖、生产方法和用途.中国,CN 1286853C,ZL 03142294.2,2004年4月7日.1-18.
    [61]Anh-Tho Nguyen,Jeanine Fontaine,Hugues Malonne,al.A sugar ester and an iridoid glycoside from Scrophularia ningpoensis[J].Phytochemistry,2005,66(10):1186-1191.
    [62]马广恩,申雅维.玄参的化学和药理研究概况[J].国外医药.物药分册,1997,12(6):251-253.
    [63]俞静静,王琴,吕圭源.玄参有效部位的药理研究进展[J].中华实用中西医杂志,2006,19(15):1866-1867.
    [64]靖会,佐建锋,李教社.苯乙醇苷类化合物的药理研究进展[J].时珍国医国药,2006,17(3):440-441
    [65]万进,方建国.环烯醚萜类化合物的研究进展[J].医药导报,2006,25(6):530-533.
    [66]陈小英,贾丽娜,刘德发,等.玄参叶的抗菌和毒性作用[J].福建中医药,1986,17(4):57.
    [67]乔卫.玄参属植物Scrophularia deserti中2个新环烯醚萜苷scropolioside D_2和harpagoside B 及其抗糖尿病和抗炎活性[J].国外医蓟植物药分册,2004,19(2):70.
    [68]张立伟,赵春贵,杨频.肉桂酸衍生物清除羟基自由基活性研究[J].中国医院药学杂志,2004,24(11):662-663.
    [69]龚维桂,钱伯初,许衡钧,等.玄参对心血管系统药理作用的研究[J].浙江医学,1981,3(1):11-13.
    [70]Huang Guo-cai,Li Yi-ming,He Xiang,et al.Effect of angroside in Scrophularia ningpoensis on synthesis of platelet cAMP and plasma PGI_2/TXA_2 in Rabbits[J].Acad J Sec Mil Med Univ,2004,25(8):920-921.
    [71]顾亚夫,赖尧基,倪正,等.脉络宁治疗三种血栓病的疗效及其机理探讨[J].中国中西医结合杂志,1987,7(2):718-720.
    [72]孙奎,姜华.玄参中苯丙素苷对肝细胞损伤保护作用的研究[J].药学实践杂志,2002,20(4):234-235.
    [73]黄才国,李医明,贺祥,等.玄参中苯丙素苷对大鼠肝损伤细胞凋亡的影响[J].中西医结合肝病杂志,2004,14(3):160-161.
    [74]王军,于震,李更生,等.地黄苷A对“阴虚”及免疫功能低下小鼠的药理作用[J].中国药学杂志,2002,37(1):20-22.
    [75]黄才国,魏善建,刘军华.玄参中环烯醚萜Zpibueropyridinium A对醛糖还原酶的抑制作用[J].第二军医大学学报,2006,27(7):760-762.
    [76]谢丽华,刘洪宇,钱瑞琴,等.哈巴苷与哈巴俄苷对阴虚小鼠免疫功能及血浆环化核苷酸的影响[J].北京大学学报(医学版),2001,33(3):283-284.
    [77]谢文光,邵宁生,马晓昌,等.玄参治疗大鼠内毒素血症的血清蛋白质组变化的初步研究[J].中国中药杂志,2004,29(9):877-882.
    [78]尚雁君,李医明,蒋山好,等.玄参中苯丙素苷Acteoside对小鼠高尿酸血症的影响[J].解放军药学学报,2006,22(1):30-32.
    [79]金戈,张婷,王涛,等.α-干扰素与肉桂酸对人肺癌细胞增殖的抑制作用[J].癌症,2002,21(8):860-862.
    [80]鲁严,朱文元,谭城.苦参碱及肉桂酸对体外培养小鼠黑素细胞黑素生成的影响[J].临床皮肤科杂志,2006,35(11):698-670.
    [81]谢文光,邵宁生,马晓昌,等.玄参治疗大鼠内毒素血症的血清蛋白质组变化的初步研究[J].中国中药杂志,2004,29(9):877-882.
    [82]尚雁君,李医明,蒋山好,等.玄参中苯丙素苷Acteoside对小鼠高尿酸血症的影响[J].解放军药学学报,2006,22(1):30-32.
    [83]王峥涛.中药质量标准研究进展与展望[J].中国天然药物,2006,4(6):403-410.国家医蓟管理局中草药情报中心站.植物药有效成分手册[M].北京:人民卫生出版社,1986:546.
    [84]中国医学科学院药物研究所等编著.中药志(第一册)[M].北京:人民卫生出版社,1979:350-354.
    [85]谢丽华,王建华,刘洪宇,等.中药玄参薄层色谱鉴别法的建立[J].中国中蓟杂志,2000,25(11):654-656.
    [86]林毅宏,陈文湛,冯品光,等.增液口服液的制备和质量标准研究[J].中国医院药学杂志,1994,14(4):165-166.
    [87]余明艳,汪水娟,汪勤等.反相高效液相色谱法测定脉络宁注射液中肉桂酸的含量[J].中国中药杂志,2000,25(7):415-416.
    [88]张洪霞,宋金玉,赵荣淑,等.RP-HPLC法同时测定玄参中肉桂酸、哈巴酯苷的含量[J].沈阳药科大学学报,2006,23(8):507-509,528.
    [89]Xie Lihua,Liu Hongyu,Xu Bingjiu,et al.HPLC Determination of Harpagoside and Cinnamic Acid in Radix Scrophulariae[J].Joumal of Chinese Pharmaceutical Sciences,2001,10(3):148-151.
    [90]贺瑶琴.土壤学与土壤理化分析[M].乌鲁木齐:新疆科技卫生出版社,1995
    [91]骆洪义,丁方军.土壤学实验[M].成都:成都科技大学出版社,1995
    [92]赵春江.数字农业信息标准_作物卷.北京[M]:中国农业出版社,2004年11_第一版
    [93]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000
    [94]杨敏,杨水平,李隆云.播期对青蒿生长和青蒿素含量的影响[D].西南大学,2008
    [95]Arnon D.I.Copper enzymes in isolated chloroplasts,polyphenol oxidase in Beta vulgaris.Plant Physiology,1949,24:1-5
    [96]吴志旭,张雅燕.浮游植物体内叶绿素a测定方法的改进[J].化学分析计量,2002,11(6):37-38
    [97]吴江生,李培武.油菜籽叶绿素测定方法研究及应用[D].华中农业大学,2008
    [98]张宪政,苏正淑.作物水分亏缺伤害生理研究概况[J].沈阳农业大学学报,1997,27(1):85-91
    [99]叶绿素提取方法及稳定性研究进展[EB].中国化工信息网,2009-5-14
    [100]国家药典委员会.中华人民共和国药典(2005年版一部)[S].北京:化学工业出版社,2005:76
    [101]赵陆华,黄泰康.中药高效液相色谱法应用[M].北京:中国医药科技出版社,2005
    [102]马顺喜,刘瑾霞.保护土壤资源的重要性[J].现代农业,2004,7:48
    [103]李西文,陈士林.遮荫下高原濒危药用植物川贝母(Fritillaria cirrhosa)光合作用和叶绿素荧光特征[J]生态学报,2008,7:57
    [104]肖风雷.玄参高产栽培技术及采收加工[J].农业科技通讯.1997,10:13
    [105]赵志新,梁宗锁.玄参种质资源多样性研究[D].西北农林科技大学,2008
    [106]李静,孙文基.中药玄参中环烯醚萜苷类成分的研究[D].西北大学,2006
    [107]王力生,郭亚健,邹节明.苦玄参的化学成分研究及应用[D].北京中医药大学,2004
    [108]白志川.不同产地与加工方法玄参中肉桂酸的含量测定[J].西南农业大学学报,28(3):425-427
    [109]http://www.zhong-yao.net/baojia/xuancan[E].中医中药网
    [110]郑智文.玄参后市价格能否趋升[J],2006,20:7-8
    [111]徐春波.本草古籍常用道地药材考[M].北京:人民卫生出版社,2007:481
    [112]唐迎雪,宋永刚.本草古籍常用药物品种与质量鉴定考[M].北京:人民卫生出版社,2007:342
    [113]李玉清.本草古籍常用药物采收加工与炮制考[M].北京:人民卫生出版社,2007:407
    [114]吴承艳,赵国平.本草古籍常用药物性味归经与功能主治对照[M].北京:人民卫生出版社,2007:391
    [115]王均宁.本草古籍常用药物配伍应用考[M].北京:人民卫生出版社,2007:319
    [116]孙洪生,严季澜.本草古籍常用药物应用禁忌考[M].北京:人民卫生出版社,2007:292
    [117]http://www.ndcnc.gov.cn[E].国家数字文化网

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