小萝卜大戟（E.rapulum Kar.et Kir.）生物活性成分的提取与分离研究
摘要
本论文主要对大戟科药用植物小萝卜大戟(E.rapulum Kar.et Kir.)提取分离实验条件的选择、化学成分进行了系统的研究,同时对大戟属植物的生理活性和临床应用进行了阐述。
本文对提取温度、提取时间、提取次数、料液比等对提取有较大影响的因素进行了分析。通过三水平四因素正交实验分析,确定了最佳工艺参数:提取温度为75℃,提取时间为75h,料液比为1:9,提取次数为5次。同时分析确定了其他参数:药材烘干温度为50～60℃,粉碎粒度60～80目,洗脱系统为石油醚-乙酸乙酯。本文使用闪式提取法和冷浸渍相结合代替了传统的提取方法,采用大溶剂瓶代替分液漏斗进行萃取,弥补了传统萃取方法中不能加热的缺点。
小萝卜大戟经提取分离得到7个化合物。经化学方法和波谱学方法鉴定了其中的4个,分别为:甾醇,阿替斯烷型化合物:atis-16-en-13(s)-hydroxy-3,14-dion,两个五环三萜齐墩果烷型,即β-香树脂型(β-amyrane)化合物,其中阿替斯烷型化合物在大戟属植物中比较少见,本论文中分离得到的两个五环三萜齐墩果烷型单体在大戟属植物中为首次发现。
This dissertation focused on the chemical studies on E.rapulum Kar.et Kir., the Physiological Activity and Application of Euphorbia L. were discussed at the same time.
This test analysised the influences on the extract effect during the extract and Chromatography with E.rapulum Kar.et Kir. ,such as :extract temperature、drying time、smashed level and solvent system.And the optimum parameters were choosed by Orthogonal Experiments Analysis of extract temperature、extract time、solid-liquid ratio、extraction times.They were temperature of 75℃, the ratio of solid to liquid of 1∶9, extract time of 75h and extraction times of 5 times.Aslo other parameters were determined : drying time 50～60℃, smashed level 60～80mu, solvent system petro ether-EtOAc.The extrat with shanshitiquqi reduced extract time and increased the extract efficiency compared with traditional method. Using new extraction method to make up for the deficiency of the traditional method.
Seven compounds were extracted and purified by silica gel column chromatography. The structures of the five compounds were determined by physico- chemical properties and spectral analyses.They were :sitosterol、atis-16-en-13(s)- hydroxy-3,14-dion、9,11-ene-β-amyrane acetate、β-amyrane acetate -11-ketone.
本文对提取温度、提取时间、提取次数、料液比等对提取有较大影响的因素进行了分析。通过三水平四因素正交实验分析,确定了最佳工艺参数:提取温度为75℃,提取时间为75h,料液比为1:9,提取次数为5次。同时分析确定了其他参数:药材烘干温度为50～60℃,粉碎粒度60～80目,洗脱系统为石油醚-乙酸乙酯。本文使用闪式提取法和冷浸渍相结合代替了传统的提取方法,采用大溶剂瓶代替分液漏斗进行萃取,弥补了传统萃取方法中不能加热的缺点。
小萝卜大戟经提取分离得到7个化合物。经化学方法和波谱学方法鉴定了其中的4个,分别为:甾醇,阿替斯烷型化合物:atis-16-en-13(s)-hydroxy-3,14-dion,两个五环三萜齐墩果烷型,即β-香树脂型(β-amyrane)化合物,其中阿替斯烷型化合物在大戟属植物中比较少见,本论文中分离得到的两个五环三萜齐墩果烷型单体在大戟属植物中为首次发现。
This dissertation focused on the chemical studies on E.rapulum Kar.et Kir., the Physiological Activity and Application of Euphorbia L. were discussed at the same time.
This test analysised the influences on the extract effect during the extract and Chromatography with E.rapulum Kar.et Kir. ,such as :extract temperature、drying time、smashed level and solvent system.And the optimum parameters were choosed by Orthogonal Experiments Analysis of extract temperature、extract time、solid-liquid ratio、extraction times.They were temperature of 75℃, the ratio of solid to liquid of 1∶9, extract time of 75h and extraction times of 5 times.Aslo other parameters were determined : drying time 50～60℃, smashed level 60～80mu, solvent system petro ether-EtOAc.The extrat with shanshitiquqi reduced extract time and increased the extract efficiency compared with traditional method. Using new extraction method to make up for the deficiency of the traditional method.
Seven compounds were extracted and purified by silica gel column chromatography. The structures of the five compounds were determined by physico- chemical properties and spectral analyses.They were :sitosterol、atis-16-en-13(s)- hydroxy-3,14-dion、9,11-ene-β-amyrane acetate、β-amyrane acetate -11-ketone.
引文
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