丹槐银屑浓缩丸对豚鼠银屑病样模型IL-8和IL-10影响的研究
|
摘要
目的：研究丹槐银屑浓缩丸对豚鼠银屑病模型IL-8和IL-10的影响,探讨其治疗银屑病的相关作用机制。为丹槐银屑浓缩丸治疗银屑病提供可靠的科学依据,同时为其在临床的广泛应用提供理论基础。
方法：5%普萘洛尔外涂豚鼠耳部皮肤,使其产生银屑病样病理改变,然后分别以丹槐银屑浓缩丸、复方青黛胶囊和甲氨喋呤注射液治疗,光镜下观察局部皮肤病理变化,ELISA法检测皮损组织IL-8和IL-10水平,观察该变化与皮损表现的关系。
结果：普萘洛尔致豚鼠银屑病模型血清和组织中的IL-8水平高于正常组(P<0.01),IL-10水平明显低于正常组(P<0.01)。MTX组、青黛组及丹槐高、中、低三个剂量组均能有效降低IL-8水平；提高IL-10水平,治疗效果明显,与模型组比较差异有显著性(P<0.05,P<0.01)且MTX、青黛胶囊和丹槐中剂量的疗效最好。
结论：丹槐银屑浓缩丸对普萘洛尔致豚鼠耳背银屑病样模型有治疗作用,其作用机理可能与其影响IL-8、IL-10水平,改变Thl/Th2的平衡有关。
Objective:To explore the effects of DAN HUAI YIN XIE Concentrated Pill on the Expression Levels of IL-8 and IL-10 in Psoriasis-like Lesions of Guinea Pigs Caused by Propranolol. Explore the mechanism related to the treatment of psoriasis. Provide reliable scientific basis,and for it's extensive application provide theoretical basis.
Methods:5% propranolo emulsion was applied on ears back of Guinea pigs to produce the psoriais-like models,then respectively treat with experimental drug. Light microscope to observe the pathological changes of local skin. Tissue lesions detected IL-8 and IL-10 levels by ELISA. Observe the performance levels and the relationship between skin lesions.
Results:Guinea pig model of psoriasis induced by propranolol in serum and tissue levels of IL-8 higher than the normal group (P<0.01), IL-10 levels were significantly lower than normal group (P<0.01). MTX group, QINGDAI group and DANHUAN high, medium and low dose groups could effectively reduce the level of IL-8; increased levels of IL-10, significant treatment effect. Compared with the model group were significantly different (P<0.05, P<0.01). Furthermore, the efficacy of the best is MTX, QINGDAI capsule and DANHUAI medium dose.
Conclusions:DAN HUAI YIN XIE Concentrated Pill have a therapeutic effect on psoriasis-like lesions of guinea pigs'ears caused by propranolol.and the mechanism may be related to influence IL-8, IL-10 levels, changing the balance of Thl/Th2.
方法：5%普萘洛尔外涂豚鼠耳部皮肤,使其产生银屑病样病理改变,然后分别以丹槐银屑浓缩丸、复方青黛胶囊和甲氨喋呤注射液治疗,光镜下观察局部皮肤病理变化,ELISA法检测皮损组织IL-8和IL-10水平,观察该变化与皮损表现的关系。
结果：普萘洛尔致豚鼠银屑病模型血清和组织中的IL-8水平高于正常组(P<0.01),IL-10水平明显低于正常组(P<0.01)。MTX组、青黛组及丹槐高、中、低三个剂量组均能有效降低IL-8水平；提高IL-10水平,治疗效果明显,与模型组比较差异有显著性(P<0.05,P<0.01)且MTX、青黛胶囊和丹槐中剂量的疗效最好。
结论：丹槐银屑浓缩丸对普萘洛尔致豚鼠耳背银屑病样模型有治疗作用,其作用机理可能与其影响IL-8、IL-10水平,改变Thl/Th2的平衡有关。
Objective:To explore the effects of DAN HUAI YIN XIE Concentrated Pill on the Expression Levels of IL-8 and IL-10 in Psoriasis-like Lesions of Guinea Pigs Caused by Propranolol. Explore the mechanism related to the treatment of psoriasis. Provide reliable scientific basis,and for it's extensive application provide theoretical basis.
Methods:5% propranolo emulsion was applied on ears back of Guinea pigs to produce the psoriais-like models,then respectively treat with experimental drug. Light microscope to observe the pathological changes of local skin. Tissue lesions detected IL-8 and IL-10 levels by ELISA. Observe the performance levels and the relationship between skin lesions.
Results:Guinea pig model of psoriasis induced by propranolol in serum and tissue levels of IL-8 higher than the normal group (P<0.01), IL-10 levels were significantly lower than normal group (P<0.01). MTX group, QINGDAI group and DANHUAN high, medium and low dose groups could effectively reduce the level of IL-8; increased levels of IL-10, significant treatment effect. Compared with the model group were significantly different (P<0.05, P<0.01). Furthermore, the efficacy of the best is MTX, QINGDAI capsule and DANHUAI medium dose.
Conclusions:DAN HUAI YIN XIE Concentrated Pill have a therapeutic effect on psoriasis-like lesions of guinea pigs'ears caused by propranolol.and the mechanism may be related to influence IL-8, IL-10 levels, changing the balance of Thl/Th2.
引文
1.唐玲,于益芝,顾军等.白介素8及其受体CXCRZ在银屑病角质形成细胞中的表达[J].中华皮肤科杂志.2002,35：105-107.
2.詹姆斯等,安德鲁斯临床皮肤病学(原书第10版).徐世正译.北京；科学出版社.2008.216-222.
3.北京中医医院.赵炳南临床经验集.人民卫生出版社,1975,211-219.
4.朱仁康,邹铭西,李博鉴.克银方治疗银屑病(牛皮癣)的临床研究.中医杂志,1981,22(4)：22.
5.金起凤.消银解毒汤治疗银屑病58例疗效观察.辽宁中医杂志,1983,7(6)：29-30.
6.赵炳南,张志礼.简明中医皮肤病学,北京：中国展望出版社,1983,200-204.
7.李林等.朱仁康老中医治疗银屑病的经验.中医杂志,1985;26(1)：12.
8.张志礼.张志礼皮肤病临床经验辑要[M].北京：中国医药科技出版社,2001,115-119.
9.顾伯华.主编实用中医外科学-治疗银屑病的经验.上海科学技术出版社,1版,1987；296.
10.秦万章.丹参在皮肤科中的应用.中华皮肤科杂志,1998,221(4)：283.
11.丁履伸,赵绚德.银屑病的中医治疗.山东中医学院学报,1980,4(4)47-49.
12.王晖等.论银屑病的主要病机为阴虚瘀热论治.中医杂志,1993；34(3)：136.
13.孙瑞珍.抗银方治疗寻常型银屑病139例疗效观察.新中医,2004,36(4)：44-45.
14.路斌,刘子航,王力.消银汤治疗寻常型银屑病76例.四川中医,2004,22(7)：79-80.
15.陈丽红.消银汤治疗寻常型银屑病65例.福建中医药杂志,2004.35(2)：26.
16.李元文,张丰川.专科专病名医临证经验丛书—皮肤病.人民卫生出版社,2002,270-340
17.张小薇.从血论治寻常性银屑病86例疗效观察.北京中医,1998,17(5)：35.
18.马绍尧,李咏梅.辨证治疗寻常型银屑病312例.湖南中医药导报,1999,5(7)：21.
19.李寿甫,杨占魁,等.脏腑辨证分型治疗银屑病1000例.陕西中医,1998,19(2)：60.
20.夏庆梅.凉血活血消银汤治疗寻常型银屑病40例.天津中医药2007,24(1)：54.
21.郭奕好,吴军等.自拟净屑饮治疗进行期寻常型银屑病30例.广西中医药,2006,29(4)：25-26.
22.王翁鸣等.自制托毒丸治疗寻常型银屑病65例报告.中国皮肤性病学杂志,1999,13(2)：108.
23.樊敏,宋宪,王卫平,等.蓝川清热合剂治疗银屑病的临床观察[J].中国中西医结合杂志,2005,25(4)：375-376.
24.杨家蕊,王永杰.银平胶囊治疗寻常型银屑病临床观察[J].中国皮肤性病学杂志,2007,21(5)：309-310.
25.曹黎生,陈其富,叶庆伦,复方丹参注射液等治疗银屑病86例[J].临床皮肤科杂志,1994,23(4)：231.
26.张君地.丹参注射液治疗寻常型银屑病58例临床报告.皮肤病与性病,1999,21(2)：168
27.艾洪亮,熊学平.复方苦参注射液治疗寻常型银屑病的临床疗效观察[J].实用中西医结合临床,2009,9(1)：57-58.
28.叶小茵,李希清,等.复方甘草酸苷治疗寻常性银屑病疗效观察.中国皮肤性病学杂志,2006,20(2)：128.
29.林能兴,涂亚庭,等.复方甘草酸苷治疗寻常型银屑病30例.医药导报,2004,23(12)：910-912.
30.Nicko loff B J.The search for pathogenic T cells and the genetic basis of psoriasis using a severe combined immuno deficient mouse model.Cutis,2000,65(2):110-114.
31.Gilhar A,David M,U llmann Y,etal.T21ymphocyte depende2nce of psoriatic pathology in human psoriatic skin grafted to SCID mice.J Invest Dermatol,1997,109(3):283-288.
32.Norris DA,Travers JB,Leung DYM.Lymphocyte activation in the pathogenesis of psoriasis.J Invest Dermatol,1997,109 (1):1-4
33.秦建中,秦俭,杨海珍等.白细胞介素8在银屑病发病机理中的作用.临床皮肤科杂志,1995;13：131-135.
34.赵辨.临床皮肤病学[M].南京：江苏科学技术出版社,第3版,2001：759-769.
35.刘瓦利,何伟.银屑病的病因与发病机制[J].中国临床医生,2009,37(8)：7-8.
36.Cheng L, Zhang SZ, Xiao CY, etal.The A5.1 allele of the majorhistocom-patibility complex class I chain-related gene A isassociated with psoria-sisvulgaris in Chinese.Br J Dermatol,2000,143(2):324-329.
37.王桂芝,崔艳霞,肖君刚.点滴型银屑病与链球菌感染关系的研究[J].中国中西医结合皮肤性病学杂志,2006,5(4)：195-196.
38.Farber EM, et al.Int J Dermatol.1990,9:418.
39.刘辅仁.实用皮肤科学[M].北京：人民卫生出版社,1999,第2版：338-342.
40.黄雯.泌乳素在银屑病发病机制中是否起作用[J].国外医学皮肤性病学分册,1999,25(3)：180.
41.杜华,张桂兰等.银屑病患者的血清甲状腺激素水平的测定[J].西北国防医学杂志,2000,21(3)：198.
42.奥多姆等.安德鲁斯临床皮肤病学.徐世正主译.第9版.北京：科学出版社,2004.223.
43.顾有守.银屑病治疗的进展[J].临床皮肤科杂志,1998,27(2)：136-137.
44.Markham T, Watson A, Rogers S.Adverse effects with long-term cyclos-porin for severe psoriasis.Clin Exp Dermatol,2002,27(2); 11;-114.
45.Reichert-Penetrat S, Trechot P, Barbaud A, etc.Bilateral femoral avasc-ular necrosis in a man with psoriasis:responsibility of topical cortico-steroids and role of cyclosporin.Dermatology,2001,203(4);35;-357.
46.何玉清,张锡宝.阿维A与甲氨喋吟治疗银屑病的疗效对比分析[J].中国皮肤性病学杂志,2005,19(6)：346-348.
47.王爱民,杨斌,刘巧.银屑病发病机制和治疗研究[J].中国热带医学,2004,4(3)：483-484.
48.赵春霞,范小莉,夏隆庆.银屑病治疗的新探索[J].国外医学皮肤性病学分册,2001,27(2)：71-73.
49.黄畋,张淑芝,孙令.心得安涂药造成豚鼠耳部银屑病样病理变化[J].中华皮肤科杂志,1991,24(2)：96—97.
50.Baker BS. Brent L. Valdimarsson H. et.al.Is epidermal cell proliferation in psoriatic skin grafts on nude mice driven by T-cell derived cytokines [J]? Br J Dermatol, 1992,126(2):105-110
51.秦伯益.新药评价概论[M].北京：人民卫生出版社,1998：339.
52.陈红,王思平.复方青黛胶囊治疗寻常型银屑病的疗效观察及其对血清IL-2、IL-8 的影响[J].中药材,2004,27(11)：885-886.
53.Irwin MF,Arthur IE,Frank KA,et al.Fitzpatrick's dermatology ingeneral medicine.5th,Mc Graw2Hill Company,New York,1999:2797-2810.
54.袁媛,朱健平,陈系古.银屑病动物模型[J].中国实验动物学报,2008,16(1)：70-71.
55.张衍国,付萌,刘玉锋SCID小鼠在银屑病发病机制研究中的应用[J].国外医学皮肤性病学分册,2000,28(4)：228-230.
56.Gilhar A, David M, Ullmann Y, et al.Dependence of psoriati pathology in human psoriatic skin grafted to SCID nice[J].J Inves Dermatol,1998,110:459-460.
57.戚春婷,张瑞忠,徐平,等.人类疾病模型小鼠的标准化维持方法研究[J].中国实验动物学报,2005,13(2)：91-96.
58.Gaylarde PM, Brock AP, Sarkany I.Psoriasiform changes in guine apig skin from propranolol.Clin Exp Dermatol[J].1978,3(2):157-160.
59.孙丽蕴,紫草素对角质形成细胞株凋亡的影响[J].中国皮肤性病学杂志,2004,18(6)：336-338.
60.王朴.生地黄的药理研究与临床应用[J].中国中医院现代远程教育,2008,6(8)：986.
61.路又璐,秦建中.17味中药对培养的表皮细胞增殖的影响[J].临床皮肤科杂志,1996：25(4)：202.
62.华东,吴明嫒,于晓红,等.赤芍总甙对荷瘤鼠细胞免疫功能的影响[J].中医药学报,2004,32(1)47-48.
63.刘超,王静,杨军.赤芍总甙活血化瘀作用的研究[J].中药材,2000,23(9)：557-560.
64.路又璐,秦建中.复方丹参注射液对表皮细胞增殖的影响.中国中西医结合杂志1996；16(1)：9.
65.周淑维,宋坪,刘瓦利.庄国康教授治疗银屑病经验[J].中国中西医结合皮肤性病学杂志2004,3(1)：38.
66.王文俊,姚智,吴咸中等.大黄素,丹参素对单核细胞分泌炎性细胞因子的调节[J].中国免疫学杂志1995,n(6)：371.
67.岳兴如,侯宗霞,刘萍,王澍嵩.白茅根抗炎的药理作用.中国临床康复,2006,10(43)：85-86.
68.吕世静、黄槐莲、袁汉尧.白茅根多糖对T淋巴细胞免疫调节效应的研究[J].中国 新药杂志,2004,13(9)：834-835
69.江苏新医学院.中药大词典[M].上海：上海人民出版社,1977.2434.
70.Kalashnikova N A,Gerashchenko G I.Antiphlogistic activity of several flavonoids[J].Aktual Vopr Farm,1974(2):352.
71.侯家玉.中药药理学.北京：中国中医药出版社,2002,71-72.
72.范斌等,凉血和活血中药对银屑病患者不同时期细胞因子的影响[J].中国中西医结合皮肤病学杂志,2006.5(2)：70-71}.
73.刘晓明等,20种中药灌胃对小鼠上皮细胞增殖和表皮细胞分化及血浆内皮素-1的影响[J].中华皮肤科杂志,2001.34(4)：282-283.
74.马清钧等.常用中药现代研究与临床.天津科技翻译出版公司.1995：162.
75.Ellis, C.N, Fradin, M.S, Messana J.M.et al {1991} Cyclosporin for plaque-type psoriasis results of a multidose, double blind mial N Engl Med,324,227-284.
76.冯素英,林麟.银屑病病情相关的实验室指标的研究[J].国外医学皮肤性病学分册,1999,25(1)：8.
2.詹姆斯等,安德鲁斯临床皮肤病学(原书第10版).徐世正译.北京；科学出版社.2008.216-222.
3.北京中医医院.赵炳南临床经验集.人民卫生出版社,1975,211-219.
4.朱仁康,邹铭西,李博鉴.克银方治疗银屑病(牛皮癣)的临床研究.中医杂志,1981,22(4)：22.
5.金起凤.消银解毒汤治疗银屑病58例疗效观察.辽宁中医杂志,1983,7(6)：29-30.
6.赵炳南,张志礼.简明中医皮肤病学,北京：中国展望出版社,1983,200-204.
7.李林等.朱仁康老中医治疗银屑病的经验.中医杂志,1985;26(1)：12.
8.张志礼.张志礼皮肤病临床经验辑要[M].北京：中国医药科技出版社,2001,115-119.
9.顾伯华.主编实用中医外科学-治疗银屑病的经验.上海科学技术出版社,1版,1987；296.
10.秦万章.丹参在皮肤科中的应用.中华皮肤科杂志,1998,221(4)：283.
11.丁履伸,赵绚德.银屑病的中医治疗.山东中医学院学报,1980,4(4)47-49.
12.王晖等.论银屑病的主要病机为阴虚瘀热论治.中医杂志,1993；34(3)：136.
13.孙瑞珍.抗银方治疗寻常型银屑病139例疗效观察.新中医,2004,36(4)：44-45.
14.路斌,刘子航,王力.消银汤治疗寻常型银屑病76例.四川中医,2004,22(7)：79-80.
15.陈丽红.消银汤治疗寻常型银屑病65例.福建中医药杂志,2004.35(2)：26.
16.李元文,张丰川.专科专病名医临证经验丛书—皮肤病.人民卫生出版社,2002,270-340
17.张小薇.从血论治寻常性银屑病86例疗效观察.北京中医,1998,17(5)：35.
18.马绍尧,李咏梅.辨证治疗寻常型银屑病312例.湖南中医药导报,1999,5(7)：21.
19.李寿甫,杨占魁,等.脏腑辨证分型治疗银屑病1000例.陕西中医,1998,19(2)：60.
20.夏庆梅.凉血活血消银汤治疗寻常型银屑病40例.天津中医药2007,24(1)：54.
21.郭奕好,吴军等.自拟净屑饮治疗进行期寻常型银屑病30例.广西中医药,2006,29(4)：25-26.
22.王翁鸣等.自制托毒丸治疗寻常型银屑病65例报告.中国皮肤性病学杂志,1999,13(2)：108.
23.樊敏,宋宪,王卫平,等.蓝川清热合剂治疗银屑病的临床观察[J].中国中西医结合杂志,2005,25(4)：375-376.
24.杨家蕊,王永杰.银平胶囊治疗寻常型银屑病临床观察[J].中国皮肤性病学杂志,2007,21(5)：309-310.
25.曹黎生,陈其富,叶庆伦,复方丹参注射液等治疗银屑病86例[J].临床皮肤科杂志,1994,23(4)：231.
26.张君地.丹参注射液治疗寻常型银屑病58例临床报告.皮肤病与性病,1999,21(2)：168
27.艾洪亮,熊学平.复方苦参注射液治疗寻常型银屑病的临床疗效观察[J].实用中西医结合临床,2009,9(1)：57-58.
28.叶小茵,李希清,等.复方甘草酸苷治疗寻常性银屑病疗效观察.中国皮肤性病学杂志,2006,20(2)：128.
29.林能兴,涂亚庭,等.复方甘草酸苷治疗寻常型银屑病30例.医药导报,2004,23(12)：910-912.
30.Nicko loff B J.The search for pathogenic T cells and the genetic basis of psoriasis using a severe combined immuno deficient mouse model.Cutis,2000,65(2):110-114.
31.Gilhar A,David M,U llmann Y,etal.T21ymphocyte depende2nce of psoriatic pathology in human psoriatic skin grafted to SCID mice.J Invest Dermatol,1997,109(3):283-288.
32.Norris DA,Travers JB,Leung DYM.Lymphocyte activation in the pathogenesis of psoriasis.J Invest Dermatol,1997,109 (1):1-4
33.秦建中,秦俭,杨海珍等.白细胞介素8在银屑病发病机理中的作用.临床皮肤科杂志,1995;13：131-135.
34.赵辨.临床皮肤病学[M].南京：江苏科学技术出版社,第3版,2001：759-769.
35.刘瓦利,何伟.银屑病的病因与发病机制[J].中国临床医生,2009,37(8)：7-8.
36.Cheng L, Zhang SZ, Xiao CY, etal.The A5.1 allele of the majorhistocom-patibility complex class I chain-related gene A isassociated with psoria-sisvulgaris in Chinese.Br J Dermatol,2000,143(2):324-329.
37.王桂芝,崔艳霞,肖君刚.点滴型银屑病与链球菌感染关系的研究[J].中国中西医结合皮肤性病学杂志,2006,5(4)：195-196.
38.Farber EM, et al.Int J Dermatol.1990,9:418.
39.刘辅仁.实用皮肤科学[M].北京：人民卫生出版社,1999,第2版：338-342.
40.黄雯.泌乳素在银屑病发病机制中是否起作用[J].国外医学皮肤性病学分册,1999,25(3)：180.
41.杜华,张桂兰等.银屑病患者的血清甲状腺激素水平的测定[J].西北国防医学杂志,2000,21(3)：198.
42.奥多姆等.安德鲁斯临床皮肤病学.徐世正主译.第9版.北京：科学出版社,2004.223.
43.顾有守.银屑病治疗的进展[J].临床皮肤科杂志,1998,27(2)：136-137.
44.Markham T, Watson A, Rogers S.Adverse effects with long-term cyclos-porin for severe psoriasis.Clin Exp Dermatol,2002,27(2); 11;-114.
45.Reichert-Penetrat S, Trechot P, Barbaud A, etc.Bilateral femoral avasc-ular necrosis in a man with psoriasis:responsibility of topical cortico-steroids and role of cyclosporin.Dermatology,2001,203(4);35;-357.
46.何玉清,张锡宝.阿维A与甲氨喋吟治疗银屑病的疗效对比分析[J].中国皮肤性病学杂志,2005,19(6)：346-348.
47.王爱民,杨斌,刘巧.银屑病发病机制和治疗研究[J].中国热带医学,2004,4(3)：483-484.
48.赵春霞,范小莉,夏隆庆.银屑病治疗的新探索[J].国外医学皮肤性病学分册,2001,27(2)：71-73.
49.黄畋,张淑芝,孙令.心得安涂药造成豚鼠耳部银屑病样病理变化[J].中华皮肤科杂志,1991,24(2)：96—97.
50.Baker BS. Brent L. Valdimarsson H. et.al.Is epidermal cell proliferation in psoriatic skin grafts on nude mice driven by T-cell derived cytokines [J]? Br J Dermatol, 1992,126(2):105-110
51.秦伯益.新药评价概论[M].北京：人民卫生出版社,1998：339.
52.陈红,王思平.复方青黛胶囊治疗寻常型银屑病的疗效观察及其对血清IL-2、IL-8 的影响[J].中药材,2004,27(11)：885-886.
53.Irwin MF,Arthur IE,Frank KA,et al.Fitzpatrick's dermatology ingeneral medicine.5th,Mc Graw2Hill Company,New York,1999:2797-2810.
54.袁媛,朱健平,陈系古.银屑病动物模型[J].中国实验动物学报,2008,16(1)：70-71.
55.张衍国,付萌,刘玉锋SCID小鼠在银屑病发病机制研究中的应用[J].国外医学皮肤性病学分册,2000,28(4)：228-230.
56.Gilhar A, David M, Ullmann Y, et al.Dependence of psoriati pathology in human psoriatic skin grafted to SCID nice[J].J Inves Dermatol,1998,110:459-460.
57.戚春婷,张瑞忠,徐平,等.人类疾病模型小鼠的标准化维持方法研究[J].中国实验动物学报,2005,13(2)：91-96.
58.Gaylarde PM, Brock AP, Sarkany I.Psoriasiform changes in guine apig skin from propranolol.Clin Exp Dermatol[J].1978,3(2):157-160.
59.孙丽蕴,紫草素对角质形成细胞株凋亡的影响[J].中国皮肤性病学杂志,2004,18(6)：336-338.
60.王朴.生地黄的药理研究与临床应用[J].中国中医院现代远程教育,2008,6(8)：986.
61.路又璐,秦建中.17味中药对培养的表皮细胞增殖的影响[J].临床皮肤科杂志,1996：25(4)：202.
62.华东,吴明嫒,于晓红,等.赤芍总甙对荷瘤鼠细胞免疫功能的影响[J].中医药学报,2004,32(1)47-48.
63.刘超,王静,杨军.赤芍总甙活血化瘀作用的研究[J].中药材,2000,23(9)：557-560.
64.路又璐,秦建中.复方丹参注射液对表皮细胞增殖的影响.中国中西医结合杂志1996；16(1)：9.
65.周淑维,宋坪,刘瓦利.庄国康教授治疗银屑病经验[J].中国中西医结合皮肤性病学杂志2004,3(1)：38.
66.王文俊,姚智,吴咸中等.大黄素,丹参素对单核细胞分泌炎性细胞因子的调节[J].中国免疫学杂志1995,n(6)：371.
67.岳兴如,侯宗霞,刘萍,王澍嵩.白茅根抗炎的药理作用.中国临床康复,2006,10(43)：85-86.
68.吕世静、黄槐莲、袁汉尧.白茅根多糖对T淋巴细胞免疫调节效应的研究[J].中国 新药杂志,2004,13(9)：834-835
69.江苏新医学院.中药大词典[M].上海：上海人民出版社,1977.2434.
70.Kalashnikova N A,Gerashchenko G I.Antiphlogistic activity of several flavonoids[J].Aktual Vopr Farm,1974(2):352.
71.侯家玉.中药药理学.北京：中国中医药出版社,2002,71-72.
72.范斌等,凉血和活血中药对银屑病患者不同时期细胞因子的影响[J].中国中西医结合皮肤病学杂志,2006.5(2)：70-71}.
73.刘晓明等,20种中药灌胃对小鼠上皮细胞增殖和表皮细胞分化及血浆内皮素-1的影响[J].中华皮肤科杂志,2001.34(4)：282-283.
74.马清钧等.常用中药现代研究与临床.天津科技翻译出版公司.1995：162.
75.Ellis, C.N, Fradin, M.S, Messana J.M.et al {1991} Cyclosporin for plaque-type psoriasis results of a multidose, double blind mial N Engl Med,324,227-284.
76.冯素英,林麟.银屑病病情相关的实验室指标的研究[J].国外医学皮肤性病学分册,1999,25(1)：8.