转ipt-bar双元基因水稻的遗传与抗冷性研究
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摘要
在田间隔离条件下,通过(转ipt-bar双元基因鄂宜105×蜀恢527)×蜀恢527]B_2F_2、B_2F_3、(转ipt-bar双元基因鄂宜105×绵恢725)×绵恢725B_2F_2、B_2F_3、转ipt-bar双元基因蜀恢527T_2、T_3等世代群体的研究,分析了ipt、bar基因在各群体的遗传规律、ipt反应阳性植株与阴性植株的抗冷性;对比研究了金23A×转ipt-bar双元基因R527和对照金优527的农艺性状,在低温冷处理下分析了转基因杂交稻与对照抗冷性的差异,结果表明:
1.ipt基因在(转ipt-bar双元基因鄂宜105×蜀恢527)×蜀恢527]B_2F_2、B_2F_3、转ipt-bar双元基因蜀恢527T_2、T_3世代群体中的分离比例为3:1符合孟德尔规律,表现单基因遗传;而在(转ipt-bar双元基因鄂宜105×绵恢725)×绵恢725B_2F_2、B_2F_3群体中分离比例不符合孟德尔规律。
2.bar基因在所研究的世代群体中,所表现出来的分离规律不一样,大多不符合3:1的分离,仅在转ipt-bar双元基因蜀恢527T_2、T_3世代群体中符合3:1的分离,表现为一对显性基因遗传。
3.在[(转ipt-bar双元基因鄂宜105×蜀恢527)×蜀恢527]B_2F_2、B_2F_3、(转ipt-bar双元基因鄂宜105×绵恢725)×绵恢725B_2F_2、B_2F_3、转ipt-bar双元基因蜀恢527T_2、T_3等世代群体中,在孕穗开花期进行低温冷处理条件下,转ipt基因阳性植株与对照相比:阳性植株的电导率和叶绿体a/b受低温的影响小于对照,而脯氨酸含量、蔗糖含量大于对照,低温对阳性植株结实率的影响小于对照材料。电导率、叶绿体a/b、脯氨酸含量、蔗糖含量及结实率的主成分分析表明:第一主成分为结实率因子,第二主成分为叶绿素因子,第三主成分为蔗糖因子。
4.在冷处理条件下,转ipt-bar双元基因植株在有效穗数、穗粒数等农艺性状表现出变异系数较大,没有规律性。
5、在孕穗开花期17℃冷处理条件下,转ipt基因阳性植株的结实率为80.08%,而金优527的结实率为28.74%,显著高于对照,表现出强抗冷性;农艺性状的相关分析表明,影响转基因金优527单株产量的主要因素是千粒重和有效穗。
Under the conditions of isolation in the field,through a number of research groups from generation to generation,analysis of the ipt,bar gene in the inheritance of all groups,ipt plants positive and negative plants' cold resistance;studied on the transfer ipt-bar dual gene R527 agronomic traits,analysis the difference of cold resistance between transgenic hybrid rice with the CK,the results showed that:
1.The separation ratio of the Ipt gene in B2F2 and B2F3 groups of(ipt-bar dual transgenic Ey 105×Shuhui 527)×Shuhui 527],T2 and T3 groups of ipt-bar dual transgenic Shuhui 527 was3:1,met the law of Mendel,the performance was single-gene,while in B2F2 and B2F3 groups of(ipt-bar dual transgenic of Ey 105×Mianhui 725)×Mianhui725,the separation ratio did not met the laws of Mendel.
2.The separation of the Bar gene did not showed the same rule,and most do not meet the ratio of 3:1,just met the ratio of 3:1 in the generation groups T2 and T3 groups of ipt-bar dual transgenic Shuhui 527,showed as heredity of a pair of dominant genes.
3.In all the generation groups which were studied,under the cold treatment of low-temperature at booting-flowering stage,compared the positive plant of ipt transgenic with CK,the effect of low-temperature on conductivity and chloroplast a/ b of positive was less than CK,and the content of proline and sugar was more than CK,the effect on the seed-setting rate was less than CK.The principal component analysis of conductivity,Chlorophyll a/b and the content of praline and sugar showed that the first principal component was seed-setting rate,the second was the Chlorophyll a/b and the third was the sugar content.
4.Under the condition of cold treatment,the plants of ipt-bar transgenic showed a greater coefficient of variation and irregular on the agronomic traits such as the amount of effective ears and seeds.
5.Under the cold treatment of 17℃-low-temperature at booting-flowering stage,the seed-setting rate of the plants of ipt-bar transgenic was 80.08%,was Significantly higher than that of CK,which was just 28.74%,showed stronger cold resistance;by analysising the agronomic traits,the results showed that the main factors which affected the yield of the single plant of transgenic Jinyou527 were grain weight and effective ear.
1.ipt基因在(转ipt-bar双元基因鄂宜105×蜀恢527)×蜀恢527]B_2F_2、B_2F_3、转ipt-bar双元基因蜀恢527T_2、T_3世代群体中的分离比例为3:1符合孟德尔规律,表现单基因遗传;而在(转ipt-bar双元基因鄂宜105×绵恢725)×绵恢725B_2F_2、B_2F_3群体中分离比例不符合孟德尔规律。
2.bar基因在所研究的世代群体中,所表现出来的分离规律不一样,大多不符合3:1的分离,仅在转ipt-bar双元基因蜀恢527T_2、T_3世代群体中符合3:1的分离,表现为一对显性基因遗传。
3.在[(转ipt-bar双元基因鄂宜105×蜀恢527)×蜀恢527]B_2F_2、B_2F_3、(转ipt-bar双元基因鄂宜105×绵恢725)×绵恢725B_2F_2、B_2F_3、转ipt-bar双元基因蜀恢527T_2、T_3等世代群体中,在孕穗开花期进行低温冷处理条件下,转ipt基因阳性植株与对照相比:阳性植株的电导率和叶绿体a/b受低温的影响小于对照,而脯氨酸含量、蔗糖含量大于对照,低温对阳性植株结实率的影响小于对照材料。电导率、叶绿体a/b、脯氨酸含量、蔗糖含量及结实率的主成分分析表明:第一主成分为结实率因子,第二主成分为叶绿素因子,第三主成分为蔗糖因子。
4.在冷处理条件下,转ipt-bar双元基因植株在有效穗数、穗粒数等农艺性状表现出变异系数较大,没有规律性。
5、在孕穗开花期17℃冷处理条件下,转ipt基因阳性植株的结实率为80.08%,而金优527的结实率为28.74%,显著高于对照,表现出强抗冷性;农艺性状的相关分析表明,影响转基因金优527单株产量的主要因素是千粒重和有效穗。
Under the conditions of isolation in the field,through a number of research groups from generation to generation,analysis of the ipt,bar gene in the inheritance of all groups,ipt plants positive and negative plants' cold resistance;studied on the transfer ipt-bar dual gene R527 agronomic traits,analysis the difference of cold resistance between transgenic hybrid rice with the CK,the results showed that:
1.The separation ratio of the Ipt gene in B2F2 and B2F3 groups of(ipt-bar dual transgenic Ey 105×Shuhui 527)×Shuhui 527],T2 and T3 groups of ipt-bar dual transgenic Shuhui 527 was3:1,met the law of Mendel,the performance was single-gene,while in B2F2 and B2F3 groups of(ipt-bar dual transgenic of Ey 105×Mianhui 725)×Mianhui725,the separation ratio did not met the laws of Mendel.
2.The separation of the Bar gene did not showed the same rule,and most do not meet the ratio of 3:1,just met the ratio of 3:1 in the generation groups T2 and T3 groups of ipt-bar dual transgenic Shuhui 527,showed as heredity of a pair of dominant genes.
3.In all the generation groups which were studied,under the cold treatment of low-temperature at booting-flowering stage,compared the positive plant of ipt transgenic with CK,the effect of low-temperature on conductivity and chloroplast a/ b of positive was less than CK,and the content of proline and sugar was more than CK,the effect on the seed-setting rate was less than CK.The principal component analysis of conductivity,Chlorophyll a/b and the content of praline and sugar showed that the first principal component was seed-setting rate,the second was the Chlorophyll a/b and the third was the sugar content.
4.Under the condition of cold treatment,the plants of ipt-bar transgenic showed a greater coefficient of variation and irregular on the agronomic traits such as the amount of effective ears and seeds.
5.Under the cold treatment of 17℃-low-temperature at booting-flowering stage,the seed-setting rate of the plants of ipt-bar transgenic was 80.08%,was Significantly higher than that of CK,which was just 28.74%,showed stronger cold resistance;by analysising the agronomic traits,the results showed that the main factors which affected the yield of the single plant of transgenic Jinyou527 were grain weight and effective ear.
引文
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