元宝枫种仁油的提取及其神经酸分离纯化的研究
|
摘要
元宝枫种仁油富含多种人体必需脂肪酸和脂溶性维生素,其中不饱和脂肪酸含量高达92%,尤其是神经酸的含量为5.52%,具有极高的保健作用。本文对元宝枫种仁油的提取及其神经酸的分离纯化进行了研究,为元宝枫种仁油和神经酸的开发利用提供了理论依据。
本文的主要研究内容有:(1)对元宝枫翅果的水分含量、翅果出仁率和种仁含油量进行了动态分析;(2)采用索氏提取法、压榨法和超声波辅助提取法对元宝枫种仁油进行提取,并对其油脂的理化指标和脂肪酸组成进行了检测;(3)采用低温结晶法和分子蒸馏法对元宝枫种仁油混合脂肪酸中神经酸的分离纯化进行了研究。(4)以小鼠为试验动物,通过水迷宫和跳台试验对元宝枫油混合脂肪酸四级蒸馏产物改善记忆的功能进行了初步研究。
研究结果如下:
(1)北京顺义地区元宝枫种仁和油脂形成、累积过程从7月底开始,翅果出仁率和种仁含油率的高峰值均出现在10月底,此时翅果出仁率为34.75%,种仁含油量为46.69%,折合成翅果含油量为16.22%。因此,在北京顺义地区,元宝枫翅果适宜采收期为10月底。且在此时期翅果水分含量在8%～9%,较适宜油脂的提取及开发利用。
(2)三种提取方法中超声波辅助提取法的提取效果较为理想,其优化工艺条件为:提取溶剂为石油醚,超声功率为80 W,超声时间为50 min,料液比为1:16,提取3次,提取得率为37.02%。用压榨法制取的元宝枫种仁油其理化指标和脂肪酸组成成分和文献值基本相符,其中北京顺义地区元宝枫种仁油中神经酸的含量(6.07%)较云南地区元宝枫种仁油中的含量(5.52%)高约10%,是提取开发神经酸的有利资源。
(3)两种分离方法中分子蒸馏法的分离效果较好。采用刮膜式分子蒸馏器得到适宜的分离条件为:系统压力0.1Pa;蒸馏温度112℃～115℃;进料温度60℃;进料速率25 mL/h～40 mL/h;刮膜器转速300 r/min。经过四级分子蒸馏,可以将原料中的神经酸由原来的6.07%提纯至41.62%,含量提高到原来的7倍,质量收率为36.5%。
为了获得较高纯度和质量收率的神经酸,应用响应面分析法(response surfaceanalysis)对第三级蒸馏的操作参数进行优化,经SAS软件分析所得第三级分子蒸馏优化条件为:采用刮膜式分子蒸馏器,系统压力0.1 Pa,蒸馏温度114.5℃,进料温度60℃,进料速率12 mL/h,刮膜器转速295 r/min。经过三级蒸馏即可将原料中的神经酸由最初的6.07%提纯浓缩至39.02%,含量提高到原含量的近6.5倍,质量收率为66.6%。
(4)通过对元宝枫种仁油混合脂肪酸四级蒸馏产物进行的小鼠水迷宫和跳台试验,可以初步认定其中神经酸对小鼠的记忆有不同程度地改善。在水迷宫试验中,各级蒸馏产物的各剂量组对小鼠的空间记忆,工作记忆以及空间辨别能力的改善较为显著;在跳台试验中,有6个剂量组对小鼠的学习和记忆过程影响比较明显,其它剂量组的效果不明显。
Seed oil of Acer truncatum Bunge is rich in fatty acids that are essential to human beings' body and fat-soluble vitamins, up to 92 % and 5.52 % of which is unsaturated fatty acids and nervonic acid respectively. It has a very beneficial effect on human health. Extraction of seed oil of Acer truncatum Bunge and separation and purification of nervonic acid was studied in this thesis in order to provide a theoretical basis for exploitation and utilization of seed oil of Acer truncatum Bunge and nervonic acid.
The following research contents were studied in this thesis: (1) Moisture content, kernel content and oil content in seed kernel of Acer truncatum Bunge samara was dynamically analyzed; (2) Seed oil of Acer truncatum Bunge was extracted by employing the methods of soxhlet extraction, pressing and ultrasound-associated extraction and its physical-chemical indicators and fatty acid components were detected; (3) Separation and purification of nervonic acid in mixed fatty acids of Acer truncatum Bunge seed oil was studied by employing the methods of low-temperature crystallization and molecular distillation; (4) The functionality of memory improving of four-level distillation products of Acer truncatum Bunge seed oil mixed fatty acids was preliminarily studied through mice water labyrinth and jumping test.
The results are as follows:
(1) The formation and accumulation process of seed kernel and oil of Acer truncatum Bunge in Beijing Shunyi starts at the end of July, and the peak values of kernel content in samara and oil content in seed kernel both occur at the end of October, when the kernel content in samara is 34.75 % and the oil content in seed kernel is 46.69 %, which is equal to the 16.22 % oil content in samara. Therefore, the end of October is the suitable harvest time of Acer truncatum Bunge samara in Beijing Shunyi. At the same time the moisture content of samara is from 8 % to 9 %, which it is quite suitable for the extraction, exploitation and utilization of seed oil.
(2) The extracting effect of ultrasound-associated extraction is better than the other two extracting methods, and the optimal extracting parameters are: the extracting solvent petroleum ether, the ultrasonic power 80 W, the ultrasonic time 50 min, the ratio of material to liquid 1:16 and extracting 3 times, the rate of extracting yield 37.02 %. The physical-chemical indicators and fatty acid components of seed oil by pressing are similar to literature values. Besides, the nervonic acid content of Acer truncatum Bunge seed oil in Beijing Shunyi (6.07 %) is 10% higher than that in Yunnan (5.52 %). Therefore, Acer truncatum Bunge seed oil is a favorable resource for extracting and exploiting nervonic acid.
(3) The separating effect of molecular distillation is the better one between the two separating methods, and the appropriate separating condition obtained by using wiped-film molecular distillation is: system pressure 0.1 Pa, distillation temperature 112℃～115℃, feed temperature 60℃, feed rate 25 mL/h～40 mL/h, and the wiper rolling speed 300 r/min. The purity of nervonic acid can be increased from 6.07 % to 41.62 % through four steps molecular distillation. The content is seven times as much as the original one and yield is 36.5 %.
The optimal condition of the third step which was optimized by using response surface analysis in order to obtain a higher purity and yield of nervonic acid and analyzed by SAS software is: using wiped-film molecular distillation, system pressure 0.1 Pa, distillation temperature 114.5℃, feed temperature 60℃, feed rate 12 mL/h, and the wiper rolling speed 295 r/min. The purity rate of nervonic acid can be increased from 6.07 % to 39.02 %. The content is nearly 6.5 times as much as the original one and yield is 66.6 %.
(4) It is preliminarily shown that nervonic acid can improve the memory of mice in varying degrees through water labyrinth and jumping testing of four level distillation products of Acer truncatum Bunge seed oil mixed fatty acids. In water labyrinth test, each dosage group of distillation product in each level can significantly improve space and working memory and the ability to identify space. In the jumping test, the influence of 6 dosage groups on the process of mice's learning and memorizing is comparatively obvious, while that of the other groups is not obvious.
本文的主要研究内容有:(1)对元宝枫翅果的水分含量、翅果出仁率和种仁含油量进行了动态分析;(2)采用索氏提取法、压榨法和超声波辅助提取法对元宝枫种仁油进行提取,并对其油脂的理化指标和脂肪酸组成进行了检测;(3)采用低温结晶法和分子蒸馏法对元宝枫种仁油混合脂肪酸中神经酸的分离纯化进行了研究。(4)以小鼠为试验动物,通过水迷宫和跳台试验对元宝枫油混合脂肪酸四级蒸馏产物改善记忆的功能进行了初步研究。
研究结果如下:
(1)北京顺义地区元宝枫种仁和油脂形成、累积过程从7月底开始,翅果出仁率和种仁含油率的高峰值均出现在10月底,此时翅果出仁率为34.75%,种仁含油量为46.69%,折合成翅果含油量为16.22%。因此,在北京顺义地区,元宝枫翅果适宜采收期为10月底。且在此时期翅果水分含量在8%～9%,较适宜油脂的提取及开发利用。
(2)三种提取方法中超声波辅助提取法的提取效果较为理想,其优化工艺条件为:提取溶剂为石油醚,超声功率为80 W,超声时间为50 min,料液比为1:16,提取3次,提取得率为37.02%。用压榨法制取的元宝枫种仁油其理化指标和脂肪酸组成成分和文献值基本相符,其中北京顺义地区元宝枫种仁油中神经酸的含量(6.07%)较云南地区元宝枫种仁油中的含量(5.52%)高约10%,是提取开发神经酸的有利资源。
(3)两种分离方法中分子蒸馏法的分离效果较好。采用刮膜式分子蒸馏器得到适宜的分离条件为:系统压力0.1Pa;蒸馏温度112℃～115℃;进料温度60℃;进料速率25 mL/h～40 mL/h;刮膜器转速300 r/min。经过四级分子蒸馏,可以将原料中的神经酸由原来的6.07%提纯至41.62%,含量提高到原来的7倍,质量收率为36.5%。
为了获得较高纯度和质量收率的神经酸,应用响应面分析法(response surfaceanalysis)对第三级蒸馏的操作参数进行优化,经SAS软件分析所得第三级分子蒸馏优化条件为:采用刮膜式分子蒸馏器,系统压力0.1 Pa,蒸馏温度114.5℃,进料温度60℃,进料速率12 mL/h,刮膜器转速295 r/min。经过三级蒸馏即可将原料中的神经酸由最初的6.07%提纯浓缩至39.02%,含量提高到原含量的近6.5倍,质量收率为66.6%。
(4)通过对元宝枫种仁油混合脂肪酸四级蒸馏产物进行的小鼠水迷宫和跳台试验,可以初步认定其中神经酸对小鼠的记忆有不同程度地改善。在水迷宫试验中,各级蒸馏产物的各剂量组对小鼠的空间记忆,工作记忆以及空间辨别能力的改善较为显著;在跳台试验中,有6个剂量组对小鼠的学习和记忆过程影响比较明显,其它剂量组的效果不明显。
Seed oil of Acer truncatum Bunge is rich in fatty acids that are essential to human beings' body and fat-soluble vitamins, up to 92 % and 5.52 % of which is unsaturated fatty acids and nervonic acid respectively. It has a very beneficial effect on human health. Extraction of seed oil of Acer truncatum Bunge and separation and purification of nervonic acid was studied in this thesis in order to provide a theoretical basis for exploitation and utilization of seed oil of Acer truncatum Bunge and nervonic acid.
The following research contents were studied in this thesis: (1) Moisture content, kernel content and oil content in seed kernel of Acer truncatum Bunge samara was dynamically analyzed; (2) Seed oil of Acer truncatum Bunge was extracted by employing the methods of soxhlet extraction, pressing and ultrasound-associated extraction and its physical-chemical indicators and fatty acid components were detected; (3) Separation and purification of nervonic acid in mixed fatty acids of Acer truncatum Bunge seed oil was studied by employing the methods of low-temperature crystallization and molecular distillation; (4) The functionality of memory improving of four-level distillation products of Acer truncatum Bunge seed oil mixed fatty acids was preliminarily studied through mice water labyrinth and jumping test.
The results are as follows:
(1) The formation and accumulation process of seed kernel and oil of Acer truncatum Bunge in Beijing Shunyi starts at the end of July, and the peak values of kernel content in samara and oil content in seed kernel both occur at the end of October, when the kernel content in samara is 34.75 % and the oil content in seed kernel is 46.69 %, which is equal to the 16.22 % oil content in samara. Therefore, the end of October is the suitable harvest time of Acer truncatum Bunge samara in Beijing Shunyi. At the same time the moisture content of samara is from 8 % to 9 %, which it is quite suitable for the extraction, exploitation and utilization of seed oil.
(2) The extracting effect of ultrasound-associated extraction is better than the other two extracting methods, and the optimal extracting parameters are: the extracting solvent petroleum ether, the ultrasonic power 80 W, the ultrasonic time 50 min, the ratio of material to liquid 1:16 and extracting 3 times, the rate of extracting yield 37.02 %. The physical-chemical indicators and fatty acid components of seed oil by pressing are similar to literature values. Besides, the nervonic acid content of Acer truncatum Bunge seed oil in Beijing Shunyi (6.07 %) is 10% higher than that in Yunnan (5.52 %). Therefore, Acer truncatum Bunge seed oil is a favorable resource for extracting and exploiting nervonic acid.
(3) The separating effect of molecular distillation is the better one between the two separating methods, and the appropriate separating condition obtained by using wiped-film molecular distillation is: system pressure 0.1 Pa, distillation temperature 112℃～115℃, feed temperature 60℃, feed rate 25 mL/h～40 mL/h, and the wiper rolling speed 300 r/min. The purity of nervonic acid can be increased from 6.07 % to 41.62 % through four steps molecular distillation. The content is seven times as much as the original one and yield is 36.5 %.
The optimal condition of the third step which was optimized by using response surface analysis in order to obtain a higher purity and yield of nervonic acid and analyzed by SAS software is: using wiped-film molecular distillation, system pressure 0.1 Pa, distillation temperature 114.5℃, feed temperature 60℃, feed rate 12 mL/h, and the wiper rolling speed 295 r/min. The purity rate of nervonic acid can be increased from 6.07 % to 39.02 %. The content is nearly 6.5 times as much as the original one and yield is 66.6 %.
(4) It is preliminarily shown that nervonic acid can improve the memory of mice in varying degrees through water labyrinth and jumping testing of four level distillation products of Acer truncatum Bunge seed oil mixed fatty acids. In water labyrinth test, each dosage group of distillation product in each level can significantly improve space and working memory and the ability to identify space. In the jumping test, the influence of 6 dosage groups on the process of mice's learning and memorizing is comparatively obvious, while that of the other groups is not obvious.
引文
[1]白寿宁.超临界CO_2萃取枸杞油研究[J].包装与食品机械,2000.18(1):18-22.
[2]曹永孝,刘静,贺浪冲等.元宝枫单宁的抗凝血作用等[J].西北药学杂志,1993.8(3):180.
[3]曹永孝,刘静,刘千勇等.元宝枫单宁的镇静、催眠、镇痛、抗凝血和止泻作用[J].中药药理与临床,1993.9(特刊):50.
[4]陈开勋等.超临界CO_2萃取火棘油[J].中国油脂,1999.24(4):43-44.
[5]丁福祺.食用油压榨法和浸出法工艺的区别[J].中国油脂,2005.30(1):5-6.
[6]狄济乐.食用油的物理精炼[J].无锡轻工大学学报,1995.10:15-17.
[7]杜润鸿.纵览螺旋榨油机[J].粮油加工与食品机械,2006(2):15-17.
[8]杜彦山,张连富.水酶法提油工艺初步研究[J].粮食与油脂,2005.6:10-12.
[9]樊金栓,李晓明,候小蕊.元宝枫油抗菌作用研究初报[A].元宝枫开发利用研究[M].西安:陕西科学技术出版社,1996:93-96.
[10]冯武文,杨村,于宏奇.分子蒸馏技术及其应用[J].化工进展,1998,17(6):26-29.
[11]冯武文,杨村,于宏奇,蒸馏技术与日用化工(1)分子蒸馏技术的原理及特点[J].日用化学工业,2002.32(5):74-76.
[12]傅颖,王茵等.神经酸的急性毒性和致突变性研究[J].浙江预防医学,2005.17(12):13-15.
[13]龚春晖.分子蒸馏技术及其在油脂工业中的应用[J].西部粮油科技,2000.25(6):25
[14]关地.水代法制取核桃油技术[J].陕西粮油科技,1995.20(2):13.
[15]高霞,仇农学,庞福科,刘雪艳.超声波辅助提取苹果籽油工艺研究[J].中国油料作物学报2007.29(1).
[16]郭炎强.水代法生产葵花籽香油的研究[J].郑州工程学院学报,2003.23(3):77-79.
[17]高彦祥.玉米胚芽油超临界CO_2工艺[J].食品研究与开发,1997.18(2):35-38.
[18]韩锋,王建民等.食品添加剂新品种——神经酸对改善记忆的影响及在益智食品方面的应用研究进展[J].中国供销商情.乳业导刊,2003(1):18-20.
[19]黄慧琴,鲍时翔.多价不饱和脂肪酸分离纯化技术进展[J].中国油脂,1999.24(2):32-34.
[20]侯镜德,骆小明等.神经酸的分离制备方法.中华人民共和国国家知识产权局.1997.9.
[21]侯镜德,袁晓悟,吴清洲.神经酸的表征[J].现代科学仪器,1996.4:29-30.
[22]候镜德,袁晓悟.金属盐沉淀法分离神经酸[J].生物技术,1996.6(1):39-41.
[23]韩军岐,张有林,李林强.超声波处理提取葵花籽油的研究[J].食品研究与开发,2004.25(6):84-89.
[24]韩军岐,张有林等,超声波处理提取葵花籽研究[J].粮食与油脂,2004,9:24-25.
[25]贺浪冲,高雯.元宝枫油乳液对艾氏腹水癌小鼠的抗肿瘤作用[A].元宝枫开发利用研究[M].西安:陕西科学技术出版社,1996.
[26]呼晓姝,王建中等.超声波辅助提取元宝枫油的研究[J].中国粮油学报,2007.22(5):98-100.
[27]黄相中,李聪,古昆等.元宝枫叶提取物的稳定性及抗氧化性活性研究[J].云南大学学报(自然科学版),2003,25(5):442-445.
[28]矫彩山,李凯峰.分子蒸馏技术及其在工业上的应用[J].2002.29(10):56.
[29]贾振宝,陶菲,于国萍.利用酶处理提高油的浸出量[J].西部粮油科技,2002.27(5):20-22.
[30]罗登林,丘泰球,卢群.超声波技术在油脂工业中的研究进展[J].油脂工程,2005(3):49-50.
[31]刘德钦,沈西林,张文韬,周继宏.元宝枫油的市场开发前景研究[J].生态经济,2005.4:111-112.
[32]李桂英,袁永俊.水酶法提取菜籽油中破乳的研究[J].食品科技,2006.3:101-103.
[33]刘红梅.分子蒸馏技术在天然产物分离与提纯方面的应用[J].河南化工,2003.4:10-11.
[34]李珺,段作营,尤新等.水酶法提取玉米胚芽油研究[J].粮食与油脂,2002.(1):5-7.
[35]缪璐,刘仲明等.利用SAS软件优化乙肝表面抗原压电免疫传感器的实验条件[J].中国卫生检验杂志,2007.17.(4):608.
[36]李林强,李建科,刘迎利.超声波处理提取华山松籽油的研究[J].西北农林科技大学学报(自然科学版),2003.31(5):115-116.
[37]栾礼侠,许松林,任艳奎.分子蒸馏技术提纯天然维生素E的工艺研究[J].中国粮油学报,2006.21(1):100-102.
[38]刘淼,裘爱泳,苗卓,金青哲.水代法制取核桃油工艺的研究及有效成分分析[J].中国油脂,2004.29(3):13-16.
[39]刘祥义,付惠等.元宝枫油理化特性及脂肪酸组成研究[J].中国油脂,2003.28(3):66.
[40]刘祥义,付惠,张加研.云南元宝枫种子含油量及其脂肪酸成分分析[J].天然产物研究与开发,2003.5(1):38-39.
[41]刘祥义,付惠等.元宝枫油理化特性及脂肪酸组成研究[J].中国油脂,2003.28(3):66.
[42]李晓燕,金鑫,陈伟红等.分子蒸馏技术原理与火险分析[J].消防技术与产品信息,2005.5:13-14.
[43]罗永珠,任玉馨等.一种用分子蒸馏技术从元宝枫油中提取神经酸的方法.中华人民共和国国家知识产权局,2007.7.
[44]刘玉兰等.油脂制取工艺学[M].化学工业出版社,北京,2006:8-9.
[45]刘元法,倪伯文,王兴国等.米糠活性物质二十八醇功能性研究[J].中国油脂,2001.26(5):63-65.
[46]李云志,曾凡骏等.元宝枫的综合开发利用[J].食品与发酵工业,2004.30(6):90.
[47]李艳菊,王姝清,贾彩霞.元宝枫翅果油脂含量及累积规律研究[J].西北林学院学报,1997.12(10):48-52.
[48]李云志,曾凡骏.元宝枫黄酮的精制[J].食品与发酵工业,2005.31(3):125-128.
[49]李兆新.鱼油高度不饱和脂肪酸工业化提取技术的研究[J].中国海洋药物,1999.(4):24-28.
[50]马柏林,梁淑芳,赵德义等.含神经酸植物的研究[J].西北植物学报,2004.24(12):2362-2365.
[51]马传国.分子蒸馏对花椒籽油脱酸的研究[J].粮油加工与食品机械,2001.(12):40-42.
[52]孟春玲,王建中,王丰俊,徐桂娟.响应面法优化超声波辅助提取沙棘籽油的工艺研究[J].北京林业大学学报,2008.30(5).
[53]马海乐,陈均,吴守一等.超临界CO_2萃取小麦胚芽油的试验研究[J].农业工程学报,1996.12(1):182-185.
[54]牛春山.陕西树木志[M].北京:中国林业出版社,1990.17-28.
[55]倪培德.油脂加工技术[M].北京:化学工业出版社,2002.12:129-130.
[56]倪培德.油脂加工技术[M].北京:化学工业出版社,2002.2:10-12.
[57]彭英利,马承愚主编.超临界流体技术应用手册[M].北京:化学工业出版社,2005.
[58]彭阳生.植物油脂加工实用技术[M].北京:金盾出版社,2003.
[59]乔国平,王兴国.分子蒸馏技术及其在油脂工业中应用[J].粮食与油脂,2002.5:26.
[60]秦国旺.元宝枫生物学特性及经济价值[J].山西林业,2003.5:28.
[61]邱宏,金国琴,金如锋,赵伟康.水迷宫重复测量数据的方差分析及其在SPSS中的实现[J].中西医结合学报,2007.5(1):101-105.
[62]孙爱东,尹卓容,蔡同一等.CO_2超临界技术提取月见草油的工艺研究[J].中国油脂,1998.23(5):40-42.
[63]孙爱东,尹卓容,蔡同一等.CO_2超临界技术提取小麦胚芽油的研究[J].食品工业科技,1997.5:68-70.
[64]时宏,郭洪.面向21世纪的分子蒸馏单甘酯工业[J].中国食品添加剂,2000.4:54-56.
[65]孙兰萍,马龙,管清美,许晖.超声波辅助提取杜仲籽油的工艺优化[J].资源开发与市场,2009.25(2).
[66]苏宜香等.膳食脂肪酸构成及适宜推荐比值的研究概况[J].中国油脂,2003.28(1):31-34.
[67]盛平想,杨鹏辉.元宝枫种子榨油试验初报[J].陕西林业科技,1998.(1):21.
[68]申烨华,张萍,郭春会.超声波强化提取扁桃油的研究[J].延安大学学报(自然科学版),2002.21(3):53-55.
[69]石岩,梅世昌.医学动物实验实用手册[M].中国农业出版社,2002:27-30.
[70]唐传核,徐建祥,彭志英.脂肪酸营养与功能的最新研究[J].中国油脂,2000.25(6):20- 23.
[71]汤天曙,张鑫,苏运东等.用水代法生产花生小磨油的研究[J].郑州轻工业学院学报,1997.12(3):23-26.
[72]吴彩娥.n-3多不饱和脂肪酸富集纯化研究进展[J].中国油脂,2005.30(12):45.
[73]王丰俊,王建中,王宪昌,王建军.超临界CO_2流体萃取核桃油工艺条件的研究[J].北京林业大学学报,2004.3.
[74]王丰俊,王建中,王宪昌,王慧强.冷榨制取与超临界CO_2萃取核桃油的氧化稳定性比较研究[J].食品科学2005.26(5):41-43.
[75]王海鸥.超临界流体萃取的基本原理及其在食品工业中的应用[J].无锡轻工业大学学报,1995.14(3):262-268.
[76]王建民.神经酸提取纯化生产工艺.中华人民共和国国家知识产权局.2003.6.
[77]王建民,黄伟素.神经酸在婴幼儿母乳化配方奶粉中的应用[J].中国乳品工业,2003.31(2):26-27.
[78]王军武,许松林,徐世民,干爱华.分子蒸馏技术的应用现状[J],化工进展,2002.21(7):501.
[79]王静,向文胜.现代农业仪器分析应用技术[M].哈尔滨:东北林业大学出版社,2000:61-62.
[80]王镜岩,朱圣庚,徐长法.生物化学[M].高等教育出版社,2002:88-89.
[81]吴鹏,张东明,张庆波.短程蒸馏原理及其工业应用[J].2000.1:49～50.
[82]尉芹,马希汉.杜仲叶和元宝枫叶提取物抗氧化性的研究[J].食品科学,1998.19(8):3-5.
[83]王瑞元.浸出法是先进的制油工艺浸出油是优质安全的食用油[J].粮食与饲料工业,2003(12):1.
[84]王素梅,王璋.水酶法提取工艺对玉米胚芽油质量的影响[J].中国油脂,2003.28(4):18-20.
[85]吴时敏.功能性油脂[M].北京:云中国轻工业出版社,2001.
[86]王性炎.一种优良的木本油料树种——五角枫[J].油脂科技,1981.6(增刊):133.
[87]王性炎.中国元宝枫[M].成都:四川民族出版社,2003.
[88]王性炎,樊金栓,王姝清.中国含神经酸植物开发利用研究[J].中国油脂,2006.31(3):69-70.
[89]王性炎,马新世等.用元宝枫油提取神经酸的工艺方法.中华人民共和国国家知识产权局.2005.4.
[90]王性炎,孙波,梁志荣等.元宝枫拷胶的制取、分析及应用[M].西安:陕西科学技术出版社,1996:97-101.
[91]王性炎,王妹清,李艳菊.元宝枫开发利用研究[M].西安:陕西科学技术出版社,1996: 17-21.
[92]王性炎,王姝清.神经酸新资源——元宝枫油[J].中国油脂,2005,30(9):62-63.
[93]王性炎,王姝清.木本油脂的化学成分与人体健康[J].经济林研究,1983.2(1):89-95.
[94]王永福.脂肪酸分离技术的研究进展[J].日用化学工业,2002.32(3):59.
[95]王瑛瑶,王璋.水酶法从花生中提取油与水解蛋白的研究[J].食品与机械,2005.21(3):17-23.
[96]熊德元,刘雄民等.结晶法分离蒜头果油中神经酸溶剂选择研究[J].广西大学学报,2004.29(1):85-88.
[97]徐桂转,刘会丽等.响应面法优化酶催化酯交换反应研究[J].化学工程,2007.35(3):66.
[98]徐生庚,裘爱泳主译,Y.H.Hui主编.贝雷:油脂化学与工艺学[M].北京:中国轻工业出版社,2001(1):25-28.
[99]徐廷志,Konta F.中国-日本槭树资源与园林[M].西安:陕西科学技术出版社,1996.
[100]徐文晖,王俊儒,梁倩.元宝枫油中神经酸的初步分离[J].中国油脂,2007.32:1 1.
[101]谢练武,郭亚平,周春山,周尽花.压榨法与蒸馏法提取柑橘香精油的比较研究[J].化学与生物工程,2005.5:17-23.
[102]杨柏崇,李元瑞.称猴桃籽的超临界二氧化碳萃取研究[J].食品科学,2003.24(7):104-107.
[103]杨柏崇,李元瑞,刘书成等.改进设计超临界流体萃取装置的几点看法[J].包装与机械,2002.23(12):66-68.
[104]杨慧萍等.水酶法提取米糠油的研究[J].食品科学,2004.25(8):106-109.
[105]喻健良,翟志勇.分子蒸馏技术的发展及研究现状[J].化学工程,2001.29(5):70.
[106]严伟,李淑芬,田松江.超声波协助提取技术[J].化工进展,2002.21(9):649.
[107]朱大沛,石铁铮.油脂制各工艺学[M].郑州:河南科学技术出版社,1991.
[108]张广栋,罗仓学,付聪等.均匀设计法优化称猴桃籽油超声波提取工艺的研究[J].粮油加工与食品机械,2005.1:53-54.
[109]赵国志.油脂脱臭馏出物的回收和利用[J].中国油脂,1997.22(2):51-53.
[110]朱海.超临界CO_2萃取精制EPA和DHA的研究.第一届全国超临界CO_2萃取技术交流会论文集.北京,1994.
[111]张慧敏,关东胜,孙容芳等.超临界二氧化碳法萃取杏仁油的研究[J].中国粮油学报,2001.16(1):17-19.
[112]张静波,詹琳,何英.西番莲籽油成分的测定及其开发利用[J].中国油脂,2000.25(6):116-118.
[113]周立信,黄凤洪,严兴初等.(?)A亚麻酸与(?)亚麻酸[J].西部粮油科技,2000.25(6):46-48.
[114]郑敏燕,超声波法提取紫丁香中丁香油得研究[J].青海师范大学学报,2003.3:32-33.
[115]郑弢,许松林.分子蒸馏提纯:娅麻酸的研究[J].化学工业与工程,2004.21(1):25.
[116]祝顺琴,谈锋,分子蒸馏技术在天然产物分离中的应用[J].精细化工,2004.21(1):48.
[117]赵文斌,谢建新,但建明.均匀设计法优化苦杏仁油超声波提取工艺[J].粮食与油脂,2001(11):4.
[118]赵文华,田维熙,张英侠,高春春,宋学英.元宝枫叶提取物的新用途.申请号/专利号:200710119485.
[119]张相年.分子蒸馏技术及其应用[J].中国食品添加剂,1996.4:26.
[120]张相年.从深海鱼油中制备提纯EPA和DHAL酯的研究[J].广东药学院学报,1999.15(3):171-173.
[121]张相年,沈鸿刚,李贤文等.含二十八烷醇的高碳醇少量制备[J].中国医药工业杂志,2002.33(3):111-113.
[122]张鑫,薛毅,苏运东等.浓香花生小磨油的工艺研究与香气成分分析[J].食品科学,1999.(4):32-34.
[123]赵亚军,吴守一,陈钧.鱼油中二十碳五烯酸和二十二碳六烯酸提取分离研究现状和进展[J].江苏理工大学学报,1996.17(4):1-6.
[124]张亚静,朱瑞芳.分子蒸馏及其应用[J].宁波高等专科学校学报,2002.14(2):1-2.
[125]赵亚萍,丁霄霖.超临界CO_2萃取分离植物甾醇的研究[J].中国油脂,1999.24(4):41-42.
[126]张忠义,雷正杰,王鹏等.分子蒸馏及其应用[J].第一军医大学学报,2000.20(5):414.
[127]A.Molero Gbmez,et al.Short communication:Recovery of grape seed oil by liquid and supercritical carbon dioxide extraction:a comparison with conventional solvent extraction[J].The Chemical Engineering Journal and the Boichemical Engineering Journal,1996.61(3):227-231.
[128]Armando,L.,Paolo,B.,& Carlo,M.A new short path distillation system applied to the reduction of cholesterol in butter and lard[J].Journal of American Oil Chemists' Society,1994.71(6):609-614.
[129]Babin F,Sarda P,Ljmasset R,et al.Nervonic acids in red blood cell sphingomyelin in premature infants:an index of myelin maturation[J].Lipids,1993.28(7):627-630.
[130]Batistella C B,Moraes E B,Filho R M,et al.Molecular distillation-rigorous modeling and simulation for recovering vitamin E from vegetal oils[J].Applied Biochemistry and Biotechnology,2002.98-100(Spring):1187-1206.
[131]Bocevska M,et al.Quality of corn germ oil obtained by aqueous enzymatic extraction[J].JAOCS,1993.70:1273-1277.
[132]Coupland,Keith,Langley,Nigel A.Use of nervonic acid and long chain fatty acids for the treatment of demyelinating disorders United States Patent 5194448
[133]E.M.C.Reis-Vasco,et al.Reverchon.Mathematical modeling and simulation of pennyroyal essential oil supercritical extraction[J].Chemical Engineering Science,2000.55(15):2917-2922.
[134]E. Reverchon, et al.Modeling and simulation of the supercritical CO_2 extraction of vegetable oils[J]. Journal of Supercritical Fluids, 2001.19 (2): 161 - 175.
[135] Chen F, Cai T Y, et al. Optimizing conditions for the purification of crude octacosanol extract from rice bran wax by molecular distillation analyzed using response surface methodology [J]. Journal of Food Engineering, 2005.70: 47-53.
[136] Farquharson J, Cockburn F, Patrick WA, Jamieson EC, Logan RW. Infant cerebral cortex phospholipid fatty-acid composition and diet.Lancet. 1992 Oct 3,340 (8823): 810-813.
[137] Farguharson J, Jamieson E C, Logaa R W, et al. Docosahexaenoic and Nervonic acids in term and preterm infant cerebral while matter[J]. Prenal Neonal Med, 1996.1:234-240.
[138] Fullbrook, P.D. The use of enzymes in the processing of oilseeds[J]. JAOCS, 1983.60:476-478.
[139] Gerd Brunner. Supercritical fluids: technology and application to food processing[J]. Journal of Food Engineering, 2005.67 (1): 21 -33.
[140]Gorodenrd, P.G. et al. Purification of Sunflower Seed Oil with the aid of Ultrasonics[J]. Maslozhirovaya Promyshlenose, 1978, (9): 7.
[141]Greenberg D B.A theoretical and experimental study of the centrifugal molecular still[J]. AICHE J,1972,18 (2) : 269-276.
[142]H.Dominguez, et al. Enzymatic pretreatment to enhance oil extraction from fruits and oilseeds:a review[J]. Food Chemistry,1994.49(3):271-286.
[143] Health Products Based On Nervonic Acid. Technology Licensing Opportunity. University of Guelph.
[144]Jerry W. King, et al.Supercritical fluid extraction of Vernonia galamensis seeds[J]. Industrial Crops and Products, 2001.14 (3): 241-249.
[145] Jose.M. del Valle, et al. Supercritical CO_2 Processing of pretreated rosehip seeds: effect of process scale on oil extraction kinetics[J].Journal of Supercritical Fluids,2004,31 (2): 159-174.
[146]J.Sineriro et al. Optimization of the enzymatic treatment during aqueous from sunflower seed[J].Food Chemistry, 1998,61(4):467-474.
[147] K T Zilch. Separation of fatty acid [J]. Journal of the American Oil Society, 1979.56(11): 739A-742A.
[148] Larkn E C, Jarrett B A, ROU A. Reduction of relative levels of nervonic to lignoceric acid in breast milk simulated infant formula[J]. Nutr. Res. 1986. 6: 309-317.
[149]Liang Jer-hour, Hwang Jucy-Sun. Fractionation of squid visceral oil ethyl esters by short-path distillation[J].Journal of the American Oil Chemists Society,2000.77 (7): 773-777.
[150] Lutisan J, Cvengros J. Mean free path of molecules on molecular distillation[J]. Chemical Engineering Journal and Biochemical Engineering Journal, 1995.56 (2): 39-50.
[151] McGlone, O.C, et al. Coconut oil extraction by a new enzymetic processing[J].Journal of Food Science, 1986.51: 695-697.
[152]M.J. Cocero, et al. Mathematical model of supercritical extraction applied to oil seed extraction by CO_2 saturated alcohol- II. Shortcut methods[J]. Journal of Supercritical Fluids, 2001.20 (3): 245-255.
[153] Martnezm, Ballabr A A, L Bernau J J. Lipids of the developing human retina Total fatty acids, plasmalogens and fatty acid composition of ethanol amine and choline phosphoglycerides[J]. J Neurosci Res. 1988. 20:484-490.
[154]Mas R, Castano G, Illnait J, et al. Effects of policosanol in patients with type II hypercholesterolemia and additional coronary risk factors[J]. Clin Pharmacol Thel, 1999.65 (4) : 439-447.
[155] N.L.Rozzi, et al. Supercritical Fluids Extraction of Essential Oil Components from Lemon-Scented Botanicals[J]. Lenbensmittel Wissenschaft und-Technologie, 2002.35 (4): 319-324.
[156] Nobuyuki Kasai, Yoshiyuki Mizushina, Fumio Sugawara, et al. Three-Dimensional Structural Model Analysis of the Binding Site of an Inhibitor, Nervonic Acid, of Both DNA Polymerase b and HIV-1 Reverse Transcriptase[J]. J. Biochem. 2002.132:819-828.
[157] Octaviomc G. Coconut oil extraction by a new enzymatical process[J]. Journal of Food Science, 1986.151: 357-361.
[158]Olsen H.S, et al. Industrial production and application of soluble enzymatic by drolgzate of soy protein[J]. Process Biochemistry, 1981.16 (7): 6-11.
[159] P.F. Martins, V.M. Ito, et al. Free fatty acid separation from vegetable oil deodorizer distillate using molecular distillation process [J]. Separation and Purification Technology. 2005.
[160] Sinclair AJ, Crawford MA. The accumulation of arachidonate and docosahexaenoate in the developing rat brain. J Neurochem, 1972.19 (7): 1753-1758.
[161]Sosuiski.K.SosuiskiFW.and Coxworth E. Carbonhydrasehy drolysis of canola to enhance oil extraction[J]. Journal of American Oil Chemist Society, 1988.65: 357-359.
[162] S.T. Jiang, P. Shao, L.J. Pan, et al. Molecular Distillation for recovering Tocopherol and Fatty . Acid Methyl Estersfrom Rapeseed Oil Deodoriser Distillate Biosystems Engineering[J].2006.93 (4): 383.
[163] Uzman L L, Ruumley M K. Changes in the composition of the developing mouse brain duing early myelination[J]. Neuroebem, 1958. 3: 170-184.
[164]W. Fred Schuring. Alcoholic Extraction of oilseed with the aid of Ultrasonics[J].Journal of American Oil Chemists Society, 1978.44 (10): 585.
[165] Wang J M, Huang W S, Feng F Q, et al. Application of nervonic acid in breast milk simulated infant formula[J]. China Dairy Industry. 2003.31: 26-29. in Chinese.
[166] Wang X Y, Fan J S, Wang S Y, Sun R C . A new resource of nervonic acid from purpleblow maple (Acer truncatum) seed oil [J].Forest Products Journal, 2006.56: 11 - 12.
[167]Yuji Shimada, Seiichi Nakai, Masaharu Suenaga, et al. Facile purification of tocopherols from soybean oil deodorizer distillate in high yield using lipase [J].JAOCS, 2000.77 (10): 1009-1013.
[168] Zhang Y, WANG Z Y, Chen X Q, Ultrasound- associated extraction of seed oil of Korean pine[J], Journal of Forestry Research,2005.16 (2): 140-142.
[2]曹永孝,刘静,贺浪冲等.元宝枫单宁的抗凝血作用等[J].西北药学杂志,1993.8(3):180.
[3]曹永孝,刘静,刘千勇等.元宝枫单宁的镇静、催眠、镇痛、抗凝血和止泻作用[J].中药药理与临床,1993.9(特刊):50.
[4]陈开勋等.超临界CO_2萃取火棘油[J].中国油脂,1999.24(4):43-44.
[5]丁福祺.食用油压榨法和浸出法工艺的区别[J].中国油脂,2005.30(1):5-6.
[6]狄济乐.食用油的物理精炼[J].无锡轻工大学学报,1995.10:15-17.
[7]杜润鸿.纵览螺旋榨油机[J].粮油加工与食品机械,2006(2):15-17.
[8]杜彦山,张连富.水酶法提油工艺初步研究[J].粮食与油脂,2005.6:10-12.
[9]樊金栓,李晓明,候小蕊.元宝枫油抗菌作用研究初报[A].元宝枫开发利用研究[M].西安:陕西科学技术出版社,1996:93-96.
[10]冯武文,杨村,于宏奇.分子蒸馏技术及其应用[J].化工进展,1998,17(6):26-29.
[11]冯武文,杨村,于宏奇,蒸馏技术与日用化工(1)分子蒸馏技术的原理及特点[J].日用化学工业,2002.32(5):74-76.
[12]傅颖,王茵等.神经酸的急性毒性和致突变性研究[J].浙江预防医学,2005.17(12):13-15.
[13]龚春晖.分子蒸馏技术及其在油脂工业中的应用[J].西部粮油科技,2000.25(6):25
[14]关地.水代法制取核桃油技术[J].陕西粮油科技,1995.20(2):13.
[15]高霞,仇农学,庞福科,刘雪艳.超声波辅助提取苹果籽油工艺研究[J].中国油料作物学报2007.29(1).
[16]郭炎强.水代法生产葵花籽香油的研究[J].郑州工程学院学报,2003.23(3):77-79.
[17]高彦祥.玉米胚芽油超临界CO_2工艺[J].食品研究与开发,1997.18(2):35-38.
[18]韩锋,王建民等.食品添加剂新品种——神经酸对改善记忆的影响及在益智食品方面的应用研究进展[J].中国供销商情.乳业导刊,2003(1):18-20.
[19]黄慧琴,鲍时翔.多价不饱和脂肪酸分离纯化技术进展[J].中国油脂,1999.24(2):32-34.
[20]侯镜德,骆小明等.神经酸的分离制备方法.中华人民共和国国家知识产权局.1997.9.
[21]侯镜德,袁晓悟,吴清洲.神经酸的表征[J].现代科学仪器,1996.4:29-30.
[22]候镜德,袁晓悟.金属盐沉淀法分离神经酸[J].生物技术,1996.6(1):39-41.
[23]韩军岐,张有林,李林强.超声波处理提取葵花籽油的研究[J].食品研究与开发,2004.25(6):84-89.
[24]韩军岐,张有林等,超声波处理提取葵花籽研究[J].粮食与油脂,2004,9:24-25.
[25]贺浪冲,高雯.元宝枫油乳液对艾氏腹水癌小鼠的抗肿瘤作用[A].元宝枫开发利用研究[M].西安:陕西科学技术出版社,1996.
[26]呼晓姝,王建中等.超声波辅助提取元宝枫油的研究[J].中国粮油学报,2007.22(5):98-100.
[27]黄相中,李聪,古昆等.元宝枫叶提取物的稳定性及抗氧化性活性研究[J].云南大学学报(自然科学版),2003,25(5):442-445.
[28]矫彩山,李凯峰.分子蒸馏技术及其在工业上的应用[J].2002.29(10):56.
[29]贾振宝,陶菲,于国萍.利用酶处理提高油的浸出量[J].西部粮油科技,2002.27(5):20-22.
[30]罗登林,丘泰球,卢群.超声波技术在油脂工业中的研究进展[J].油脂工程,2005(3):49-50.
[31]刘德钦,沈西林,张文韬,周继宏.元宝枫油的市场开发前景研究[J].生态经济,2005.4:111-112.
[32]李桂英,袁永俊.水酶法提取菜籽油中破乳的研究[J].食品科技,2006.3:101-103.
[33]刘红梅.分子蒸馏技术在天然产物分离与提纯方面的应用[J].河南化工,2003.4:10-11.
[34]李珺,段作营,尤新等.水酶法提取玉米胚芽油研究[J].粮食与油脂,2002.(1):5-7.
[35]缪璐,刘仲明等.利用SAS软件优化乙肝表面抗原压电免疫传感器的实验条件[J].中国卫生检验杂志,2007.17.(4):608.
[36]李林强,李建科,刘迎利.超声波处理提取华山松籽油的研究[J].西北农林科技大学学报(自然科学版),2003.31(5):115-116.
[37]栾礼侠,许松林,任艳奎.分子蒸馏技术提纯天然维生素E的工艺研究[J].中国粮油学报,2006.21(1):100-102.
[38]刘淼,裘爱泳,苗卓,金青哲.水代法制取核桃油工艺的研究及有效成分分析[J].中国油脂,2004.29(3):13-16.
[39]刘祥义,付惠等.元宝枫油理化特性及脂肪酸组成研究[J].中国油脂,2003.28(3):66.
[40]刘祥义,付惠,张加研.云南元宝枫种子含油量及其脂肪酸成分分析[J].天然产物研究与开发,2003.5(1):38-39.
[41]刘祥义,付惠等.元宝枫油理化特性及脂肪酸组成研究[J].中国油脂,2003.28(3):66.
[42]李晓燕,金鑫,陈伟红等.分子蒸馏技术原理与火险分析[J].消防技术与产品信息,2005.5:13-14.
[43]罗永珠,任玉馨等.一种用分子蒸馏技术从元宝枫油中提取神经酸的方法.中华人民共和国国家知识产权局,2007.7.
[44]刘玉兰等.油脂制取工艺学[M].化学工业出版社,北京,2006:8-9.
[45]刘元法,倪伯文,王兴国等.米糠活性物质二十八醇功能性研究[J].中国油脂,2001.26(5):63-65.
[46]李云志,曾凡骏等.元宝枫的综合开发利用[J].食品与发酵工业,2004.30(6):90.
[47]李艳菊,王姝清,贾彩霞.元宝枫翅果油脂含量及累积规律研究[J].西北林学院学报,1997.12(10):48-52.
[48]李云志,曾凡骏.元宝枫黄酮的精制[J].食品与发酵工业,2005.31(3):125-128.
[49]李兆新.鱼油高度不饱和脂肪酸工业化提取技术的研究[J].中国海洋药物,1999.(4):24-28.
[50]马柏林,梁淑芳,赵德义等.含神经酸植物的研究[J].西北植物学报,2004.24(12):2362-2365.
[51]马传国.分子蒸馏对花椒籽油脱酸的研究[J].粮油加工与食品机械,2001.(12):40-42.
[52]孟春玲,王建中,王丰俊,徐桂娟.响应面法优化超声波辅助提取沙棘籽油的工艺研究[J].北京林业大学学报,2008.30(5).
[53]马海乐,陈均,吴守一等.超临界CO_2萃取小麦胚芽油的试验研究[J].农业工程学报,1996.12(1):182-185.
[54]牛春山.陕西树木志[M].北京:中国林业出版社,1990.17-28.
[55]倪培德.油脂加工技术[M].北京:化学工业出版社,2002.12:129-130.
[56]倪培德.油脂加工技术[M].北京:化学工业出版社,2002.2:10-12.
[57]彭英利,马承愚主编.超临界流体技术应用手册[M].北京:化学工业出版社,2005.
[58]彭阳生.植物油脂加工实用技术[M].北京:金盾出版社,2003.
[59]乔国平,王兴国.分子蒸馏技术及其在油脂工业中应用[J].粮食与油脂,2002.5:26.
[60]秦国旺.元宝枫生物学特性及经济价值[J].山西林业,2003.5:28.
[61]邱宏,金国琴,金如锋,赵伟康.水迷宫重复测量数据的方差分析及其在SPSS中的实现[J].中西医结合学报,2007.5(1):101-105.
[62]孙爱东,尹卓容,蔡同一等.CO_2超临界技术提取月见草油的工艺研究[J].中国油脂,1998.23(5):40-42.
[63]孙爱东,尹卓容,蔡同一等.CO_2超临界技术提取小麦胚芽油的研究[J].食品工业科技,1997.5:68-70.
[64]时宏,郭洪.面向21世纪的分子蒸馏单甘酯工业[J].中国食品添加剂,2000.4:54-56.
[65]孙兰萍,马龙,管清美,许晖.超声波辅助提取杜仲籽油的工艺优化[J].资源开发与市场,2009.25(2).
[66]苏宜香等.膳食脂肪酸构成及适宜推荐比值的研究概况[J].中国油脂,2003.28(1):31-34.
[67]盛平想,杨鹏辉.元宝枫种子榨油试验初报[J].陕西林业科技,1998.(1):21.
[68]申烨华,张萍,郭春会.超声波强化提取扁桃油的研究[J].延安大学学报(自然科学版),2002.21(3):53-55.
[69]石岩,梅世昌.医学动物实验实用手册[M].中国农业出版社,2002:27-30.
[70]唐传核,徐建祥,彭志英.脂肪酸营养与功能的最新研究[J].中国油脂,2000.25(6):20- 23.
[71]汤天曙,张鑫,苏运东等.用水代法生产花生小磨油的研究[J].郑州轻工业学院学报,1997.12(3):23-26.
[72]吴彩娥.n-3多不饱和脂肪酸富集纯化研究进展[J].中国油脂,2005.30(12):45.
[73]王丰俊,王建中,王宪昌,王建军.超临界CO_2流体萃取核桃油工艺条件的研究[J].北京林业大学学报,2004.3.
[74]王丰俊,王建中,王宪昌,王慧强.冷榨制取与超临界CO_2萃取核桃油的氧化稳定性比较研究[J].食品科学2005.26(5):41-43.
[75]王海鸥.超临界流体萃取的基本原理及其在食品工业中的应用[J].无锡轻工业大学学报,1995.14(3):262-268.
[76]王建民.神经酸提取纯化生产工艺.中华人民共和国国家知识产权局.2003.6.
[77]王建民,黄伟素.神经酸在婴幼儿母乳化配方奶粉中的应用[J].中国乳品工业,2003.31(2):26-27.
[78]王军武,许松林,徐世民,干爱华.分子蒸馏技术的应用现状[J],化工进展,2002.21(7):501.
[79]王静,向文胜.现代农业仪器分析应用技术[M].哈尔滨:东北林业大学出版社,2000:61-62.
[80]王镜岩,朱圣庚,徐长法.生物化学[M].高等教育出版社,2002:88-89.
[81]吴鹏,张东明,张庆波.短程蒸馏原理及其工业应用[J].2000.1:49～50.
[82]尉芹,马希汉.杜仲叶和元宝枫叶提取物抗氧化性的研究[J].食品科学,1998.19(8):3-5.
[83]王瑞元.浸出法是先进的制油工艺浸出油是优质安全的食用油[J].粮食与饲料工业,2003(12):1.
[84]王素梅,王璋.水酶法提取工艺对玉米胚芽油质量的影响[J].中国油脂,2003.28(4):18-20.
[85]吴时敏.功能性油脂[M].北京:云中国轻工业出版社,2001.
[86]王性炎.一种优良的木本油料树种——五角枫[J].油脂科技,1981.6(增刊):133.
[87]王性炎.中国元宝枫[M].成都:四川民族出版社,2003.
[88]王性炎,樊金栓,王姝清.中国含神经酸植物开发利用研究[J].中国油脂,2006.31(3):69-70.
[89]王性炎,马新世等.用元宝枫油提取神经酸的工艺方法.中华人民共和国国家知识产权局.2005.4.
[90]王性炎,孙波,梁志荣等.元宝枫拷胶的制取、分析及应用[M].西安:陕西科学技术出版社,1996:97-101.
[91]王性炎,王妹清,李艳菊.元宝枫开发利用研究[M].西安:陕西科学技术出版社,1996: 17-21.
[92]王性炎,王姝清.神经酸新资源——元宝枫油[J].中国油脂,2005,30(9):62-63.
[93]王性炎,王姝清.木本油脂的化学成分与人体健康[J].经济林研究,1983.2(1):89-95.
[94]王永福.脂肪酸分离技术的研究进展[J].日用化学工业,2002.32(3):59.
[95]王瑛瑶,王璋.水酶法从花生中提取油与水解蛋白的研究[J].食品与机械,2005.21(3):17-23.
[96]熊德元,刘雄民等.结晶法分离蒜头果油中神经酸溶剂选择研究[J].广西大学学报,2004.29(1):85-88.
[97]徐桂转,刘会丽等.响应面法优化酶催化酯交换反应研究[J].化学工程,2007.35(3):66.
[98]徐生庚,裘爱泳主译,Y.H.Hui主编.贝雷:油脂化学与工艺学[M].北京:中国轻工业出版社,2001(1):25-28.
[99]徐廷志,Konta F.中国-日本槭树资源与园林[M].西安:陕西科学技术出版社,1996.
[100]徐文晖,王俊儒,梁倩.元宝枫油中神经酸的初步分离[J].中国油脂,2007.32:1 1.
[101]谢练武,郭亚平,周春山,周尽花.压榨法与蒸馏法提取柑橘香精油的比较研究[J].化学与生物工程,2005.5:17-23.
[102]杨柏崇,李元瑞.称猴桃籽的超临界二氧化碳萃取研究[J].食品科学,2003.24(7):104-107.
[103]杨柏崇,李元瑞,刘书成等.改进设计超临界流体萃取装置的几点看法[J].包装与机械,2002.23(12):66-68.
[104]杨慧萍等.水酶法提取米糠油的研究[J].食品科学,2004.25(8):106-109.
[105]喻健良,翟志勇.分子蒸馏技术的发展及研究现状[J].化学工程,2001.29(5):70.
[106]严伟,李淑芬,田松江.超声波协助提取技术[J].化工进展,2002.21(9):649.
[107]朱大沛,石铁铮.油脂制各工艺学[M].郑州:河南科学技术出版社,1991.
[108]张广栋,罗仓学,付聪等.均匀设计法优化称猴桃籽油超声波提取工艺的研究[J].粮油加工与食品机械,2005.1:53-54.
[109]赵国志.油脂脱臭馏出物的回收和利用[J].中国油脂,1997.22(2):51-53.
[110]朱海.超临界CO_2萃取精制EPA和DHA的研究.第一届全国超临界CO_2萃取技术交流会论文集.北京,1994.
[111]张慧敏,关东胜,孙容芳等.超临界二氧化碳法萃取杏仁油的研究[J].中国粮油学报,2001.16(1):17-19.
[112]张静波,詹琳,何英.西番莲籽油成分的测定及其开发利用[J].中国油脂,2000.25(6):116-118.
[113]周立信,黄凤洪,严兴初等.(?)A亚麻酸与(?)亚麻酸[J].西部粮油科技,2000.25(6):46-48.
[114]郑敏燕,超声波法提取紫丁香中丁香油得研究[J].青海师范大学学报,2003.3:32-33.
[115]郑弢,许松林.分子蒸馏提纯:娅麻酸的研究[J].化学工业与工程,2004.21(1):25.
[116]祝顺琴,谈锋,分子蒸馏技术在天然产物分离中的应用[J].精细化工,2004.21(1):48.
[117]赵文斌,谢建新,但建明.均匀设计法优化苦杏仁油超声波提取工艺[J].粮食与油脂,2001(11):4.
[118]赵文华,田维熙,张英侠,高春春,宋学英.元宝枫叶提取物的新用途.申请号/专利号:200710119485.
[119]张相年.分子蒸馏技术及其应用[J].中国食品添加剂,1996.4:26.
[120]张相年.从深海鱼油中制备提纯EPA和DHAL酯的研究[J].广东药学院学报,1999.15(3):171-173.
[121]张相年,沈鸿刚,李贤文等.含二十八烷醇的高碳醇少量制备[J].中国医药工业杂志,2002.33(3):111-113.
[122]张鑫,薛毅,苏运东等.浓香花生小磨油的工艺研究与香气成分分析[J].食品科学,1999.(4):32-34.
[123]赵亚军,吴守一,陈钧.鱼油中二十碳五烯酸和二十二碳六烯酸提取分离研究现状和进展[J].江苏理工大学学报,1996.17(4):1-6.
[124]张亚静,朱瑞芳.分子蒸馏及其应用[J].宁波高等专科学校学报,2002.14(2):1-2.
[125]赵亚萍,丁霄霖.超临界CO_2萃取分离植物甾醇的研究[J].中国油脂,1999.24(4):41-42.
[126]张忠义,雷正杰,王鹏等.分子蒸馏及其应用[J].第一军医大学学报,2000.20(5):414.
[127]A.Molero Gbmez,et al.Short communication:Recovery of grape seed oil by liquid and supercritical carbon dioxide extraction:a comparison with conventional solvent extraction[J].The Chemical Engineering Journal and the Boichemical Engineering Journal,1996.61(3):227-231.
[128]Armando,L.,Paolo,B.,& Carlo,M.A new short path distillation system applied to the reduction of cholesterol in butter and lard[J].Journal of American Oil Chemists' Society,1994.71(6):609-614.
[129]Babin F,Sarda P,Ljmasset R,et al.Nervonic acids in red blood cell sphingomyelin in premature infants:an index of myelin maturation[J].Lipids,1993.28(7):627-630.
[130]Batistella C B,Moraes E B,Filho R M,et al.Molecular distillation-rigorous modeling and simulation for recovering vitamin E from vegetal oils[J].Applied Biochemistry and Biotechnology,2002.98-100(Spring):1187-1206.
[131]Bocevska M,et al.Quality of corn germ oil obtained by aqueous enzymatic extraction[J].JAOCS,1993.70:1273-1277.
[132]Coupland,Keith,Langley,Nigel A.Use of nervonic acid and long chain fatty acids for the treatment of demyelinating disorders United States Patent 5194448
[133]E.M.C.Reis-Vasco,et al.Reverchon.Mathematical modeling and simulation of pennyroyal essential oil supercritical extraction[J].Chemical Engineering Science,2000.55(15):2917-2922.
[134]E. Reverchon, et al.Modeling and simulation of the supercritical CO_2 extraction of vegetable oils[J]. Journal of Supercritical Fluids, 2001.19 (2): 161 - 175.
[135] Chen F, Cai T Y, et al. Optimizing conditions for the purification of crude octacosanol extract from rice bran wax by molecular distillation analyzed using response surface methodology [J]. Journal of Food Engineering, 2005.70: 47-53.
[136] Farquharson J, Cockburn F, Patrick WA, Jamieson EC, Logan RW. Infant cerebral cortex phospholipid fatty-acid composition and diet.Lancet. 1992 Oct 3,340 (8823): 810-813.
[137] Farguharson J, Jamieson E C, Logaa R W, et al. Docosahexaenoic and Nervonic acids in term and preterm infant cerebral while matter[J]. Prenal Neonal Med, 1996.1:234-240.
[138] Fullbrook, P.D. The use of enzymes in the processing of oilseeds[J]. JAOCS, 1983.60:476-478.
[139] Gerd Brunner. Supercritical fluids: technology and application to food processing[J]. Journal of Food Engineering, 2005.67 (1): 21 -33.
[140]Gorodenrd, P.G. et al. Purification of Sunflower Seed Oil with the aid of Ultrasonics[J]. Maslozhirovaya Promyshlenose, 1978, (9): 7.
[141]Greenberg D B.A theoretical and experimental study of the centrifugal molecular still[J]. AICHE J,1972,18 (2) : 269-276.
[142]H.Dominguez, et al. Enzymatic pretreatment to enhance oil extraction from fruits and oilseeds:a review[J]. Food Chemistry,1994.49(3):271-286.
[143] Health Products Based On Nervonic Acid. Technology Licensing Opportunity. University of Guelph.
[144]Jerry W. King, et al.Supercritical fluid extraction of Vernonia galamensis seeds[J]. Industrial Crops and Products, 2001.14 (3): 241-249.
[145] Jose.M. del Valle, et al. Supercritical CO_2 Processing of pretreated rosehip seeds: effect of process scale on oil extraction kinetics[J].Journal of Supercritical Fluids,2004,31 (2): 159-174.
[146]J.Sineriro et al. Optimization of the enzymatic treatment during aqueous from sunflower seed[J].Food Chemistry, 1998,61(4):467-474.
[147] K T Zilch. Separation of fatty acid [J]. Journal of the American Oil Society, 1979.56(11): 739A-742A.
[148] Larkn E C, Jarrett B A, ROU A. Reduction of relative levels of nervonic to lignoceric acid in breast milk simulated infant formula[J]. Nutr. Res. 1986. 6: 309-317.
[149]Liang Jer-hour, Hwang Jucy-Sun. Fractionation of squid visceral oil ethyl esters by short-path distillation[J].Journal of the American Oil Chemists Society,2000.77 (7): 773-777.
[150] Lutisan J, Cvengros J. Mean free path of molecules on molecular distillation[J]. Chemical Engineering Journal and Biochemical Engineering Journal, 1995.56 (2): 39-50.
[151] McGlone, O.C, et al. Coconut oil extraction by a new enzymetic processing[J].Journal of Food Science, 1986.51: 695-697.
[152]M.J. Cocero, et al. Mathematical model of supercritical extraction applied to oil seed extraction by CO_2 saturated alcohol- II. Shortcut methods[J]. Journal of Supercritical Fluids, 2001.20 (3): 245-255.
[153] Martnezm, Ballabr A A, L Bernau J J. Lipids of the developing human retina Total fatty acids, plasmalogens and fatty acid composition of ethanol amine and choline phosphoglycerides[J]. J Neurosci Res. 1988. 20:484-490.
[154]Mas R, Castano G, Illnait J, et al. Effects of policosanol in patients with type II hypercholesterolemia and additional coronary risk factors[J]. Clin Pharmacol Thel, 1999.65 (4) : 439-447.
[155] N.L.Rozzi, et al. Supercritical Fluids Extraction of Essential Oil Components from Lemon-Scented Botanicals[J]. Lenbensmittel Wissenschaft und-Technologie, 2002.35 (4): 319-324.
[156] Nobuyuki Kasai, Yoshiyuki Mizushina, Fumio Sugawara, et al. Three-Dimensional Structural Model Analysis of the Binding Site of an Inhibitor, Nervonic Acid, of Both DNA Polymerase b and HIV-1 Reverse Transcriptase[J]. J. Biochem. 2002.132:819-828.
[157] Octaviomc G. Coconut oil extraction by a new enzymatical process[J]. Journal of Food Science, 1986.151: 357-361.
[158]Olsen H.S, et al. Industrial production and application of soluble enzymatic by drolgzate of soy protein[J]. Process Biochemistry, 1981.16 (7): 6-11.
[159] P.F. Martins, V.M. Ito, et al. Free fatty acid separation from vegetable oil deodorizer distillate using molecular distillation process [J]. Separation and Purification Technology. 2005.
[160] Sinclair AJ, Crawford MA. The accumulation of arachidonate and docosahexaenoate in the developing rat brain. J Neurochem, 1972.19 (7): 1753-1758.
[161]Sosuiski.K.SosuiskiFW.and Coxworth E. Carbonhydrasehy drolysis of canola to enhance oil extraction[J]. Journal of American Oil Chemist Society, 1988.65: 357-359.
[162] S.T. Jiang, P. Shao, L.J. Pan, et al. Molecular Distillation for recovering Tocopherol and Fatty . Acid Methyl Estersfrom Rapeseed Oil Deodoriser Distillate Biosystems Engineering[J].2006.93 (4): 383.
[163] Uzman L L, Ruumley M K. Changes in the composition of the developing mouse brain duing early myelination[J]. Neuroebem, 1958. 3: 170-184.
[164]W. Fred Schuring. Alcoholic Extraction of oilseed with the aid of Ultrasonics[J].Journal of American Oil Chemists Society, 1978.44 (10): 585.
[165] Wang J M, Huang W S, Feng F Q, et al. Application of nervonic acid in breast milk simulated infant formula[J]. China Dairy Industry. 2003.31: 26-29. in Chinese.
[166] Wang X Y, Fan J S, Wang S Y, Sun R C . A new resource of nervonic acid from purpleblow maple (Acer truncatum) seed oil [J].Forest Products Journal, 2006.56: 11 - 12.
[167]Yuji Shimada, Seiichi Nakai, Masaharu Suenaga, et al. Facile purification of tocopherols from soybean oil deodorizer distillate in high yield using lipase [J].JAOCS, 2000.77 (10): 1009-1013.
[168] Zhang Y, WANG Z Y, Chen X Q, Ultrasound- associated extraction of seed oil of Korean pine[J], Journal of Forestry Research,2005.16 (2): 140-142.