非淀粉多糖酶制剂对樱桃谷鸭日粮能量代谢调控的研究
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摘要
根据玉米—豆粕日粮中主要非淀粉多糖的种类和含量，以能量消化率为标识，利用离体消化技术，结合生物学试验，用体外法评定非淀粉多糖酶制剂对樱桃谷鸭日粮能量代谢调控的研究。
试验一采用单因素完全随机试验设计，选用了四种非淀粉多糖酶(NSP enzyme)，即木聚糖酶、β-葡聚糖酶、纤维素酶和果胶酶，利用离体消化技术，探讨了它们对玉米—豆粕日粮能量的调控效应，并以此为基础，利用四因素(1／2实施)二次回归通用旋转组合设计，分别设定5个添加水平，对四种酶的最佳酶谱组合进行了筛选。
试验二选用了三组复合酶(X1、X2、X3)添加在日粮中。NSP复合酶X1由木聚糖酶700μg／g、β-葡聚糖酶400μg／g、纤维素酶700μg／g、果胶酶400μg／g组成。NSP复合酶X2由木聚糖酶500μg／g、β-葡聚糖酶600μg／g、纤维素酶500μg／g、果胶酶600μg／g组成。NSP复合酶X3由木聚糖酶500μg／g、β-葡聚糖酶600μg／g、纤维素酶700μg／g、果胶酶400μg／g组成。分别为试验B、C、D组。利用强饲排空法验证该复合酶对日粮代谢能和日粮干物质消化率的调控效应。动物试验采用随机区组试验设计，将48只樱桃谷公鸭分为4个处理组，每组设4个重复，每重复3只鸭。
试验结果表明，NSP复合酶制剂可显著提高肉鸭玉米-豆粕-杂粕日粮的AME和DMD(P＜0.05)，且AME与DMD成正比。NSP复合酶对AME和DMD的调控效应大小顺序：NSP复合X2＞NSP复合X1＞NSP复合X3；试验组B、C、D较对照组A的AME和DMD分别提高了0.638MJ／kg和8.84％、0.96MJ／kg和11.27％、0.479MJ／kg和4.04％；在该试验条件下，最佳酶谱组合为：木聚糖酶757239.5U／kg、β-葡聚糖酶2198766.8U／kg、纤维素酶4542.9U／kg和果胶酶3903.2U／kg；对日粮能量调控效应的主次顺序为木聚糖酶＞β-葡聚糖酶＞纤维素酶＞果胶酶，且它们调控肉鸭日粮能量的最适添加量分别为600μg／g、500μg／g、500μg／g、600μg／g。
According to species and contents of Non Starch Polysaccharides (NSP) incorn-soybean diets, Energy digestibility was marked, Studies on the effects of nonstarch polysaccharide enzyme on energy regulation in cherry valley ducklings dietswith a vitro and vivo method.
The first experiment aimed to study the dose-response of four Non StarchPolysaccharides (NSP) enzymes (xylanse;β-glucanase; cellulose; pectase) on theenergy in ducklings diets and sceen the optimum enzyme combination by in vitromethod. the NSP enzyme on the energy in diet using One-way randomizedexperimental design, then, screened the optimum enzyme combination rising fourfactors regress-general rotary design by in vitro method. The result showed that theoptimum enzyme combination was xylanase 757239.5U/kg,β-glucanase2198766.8U/kg, cellulose 4542.9U/kg and pectase 3903.2U/kg. The effect of NSPenzyme on regulation of energy in diet: xylanase＞β-glucanase＞cellulose＞pectase.The best of accession quantity is 600μg/g、500μg/g、500μg/g、600μg/grespectively.
The second experiment was conducted to study three group compound enzymes toregulate the effect of diet metabolizable energy and DM digestibility by force feeding-empting method. Animal is used One-way randomized blocks experimental design.The cherry valley ducklings was conducted at 4 treatments, each treatment had4duplicates,each duplicate had 3 ducklings with male. The result suggested NSPenzyme complex improved apparent metabolizable energy and digestibility of DMin the ducklings diets(P＜0.05); And apparent metabolizable energy and digestibilityof DM had direct ratio. The effect of compound enzyme on regulation of apparentmetabolizable energy and digestibility of DM:NSP X_2＞NSP X_1＞NSPX_3.The trailtreatment B、C、D of apparent metabolizable energy and DM digestibility wasimproved 0.638MJ/kg 8.84％、0.96MJ/kg 11.27％、0.479 MJ/kg 4.04％to the contratA.
试验一采用单因素完全随机试验设计，选用了四种非淀粉多糖酶(NSP enzyme)，即木聚糖酶、β-葡聚糖酶、纤维素酶和果胶酶，利用离体消化技术，探讨了它们对玉米—豆粕日粮能量的调控效应，并以此为基础，利用四因素(1／2实施)二次回归通用旋转组合设计，分别设定5个添加水平，对四种酶的最佳酶谱组合进行了筛选。
试验二选用了三组复合酶(X1、X2、X3)添加在日粮中。NSP复合酶X1由木聚糖酶700μg／g、β-葡聚糖酶400μg／g、纤维素酶700μg／g、果胶酶400μg／g组成。NSP复合酶X2由木聚糖酶500μg／g、β-葡聚糖酶600μg／g、纤维素酶500μg／g、果胶酶600μg／g组成。NSP复合酶X3由木聚糖酶500μg／g、β-葡聚糖酶600μg／g、纤维素酶700μg／g、果胶酶400μg／g组成。分别为试验B、C、D组。利用强饲排空法验证该复合酶对日粮代谢能和日粮干物质消化率的调控效应。动物试验采用随机区组试验设计，将48只樱桃谷公鸭分为4个处理组，每组设4个重复，每重复3只鸭。
试验结果表明，NSP复合酶制剂可显著提高肉鸭玉米-豆粕-杂粕日粮的AME和DMD(P＜0.05)，且AME与DMD成正比。NSP复合酶对AME和DMD的调控效应大小顺序：NSP复合X2＞NSP复合X1＞NSP复合X3；试验组B、C、D较对照组A的AME和DMD分别提高了0.638MJ／kg和8.84％、0.96MJ／kg和11.27％、0.479MJ／kg和4.04％；在该试验条件下，最佳酶谱组合为：木聚糖酶757239.5U／kg、β-葡聚糖酶2198766.8U／kg、纤维素酶4542.9U／kg和果胶酶3903.2U／kg；对日粮能量调控效应的主次顺序为木聚糖酶＞β-葡聚糖酶＞纤维素酶＞果胶酶，且它们调控肉鸭日粮能量的最适添加量分别为600μg／g、500μg／g、500μg／g、600μg／g。
According to species and contents of Non Starch Polysaccharides (NSP) incorn-soybean diets, Energy digestibility was marked, Studies on the effects of nonstarch polysaccharide enzyme on energy regulation in cherry valley ducklings dietswith a vitro and vivo method.
The first experiment aimed to study the dose-response of four Non StarchPolysaccharides (NSP) enzymes (xylanse;β-glucanase; cellulose; pectase) on theenergy in ducklings diets and sceen the optimum enzyme combination by in vitromethod. the NSP enzyme on the energy in diet using One-way randomizedexperimental design, then, screened the optimum enzyme combination rising fourfactors regress-general rotary design by in vitro method. The result showed that theoptimum enzyme combination was xylanase 757239.5U/kg,β-glucanase2198766.8U/kg, cellulose 4542.9U/kg and pectase 3903.2U/kg. The effect of NSPenzyme on regulation of energy in diet: xylanase＞β-glucanase＞cellulose＞pectase.The best of accession quantity is 600μg/g、500μg/g、500μg/g、600μg/grespectively.
The second experiment was conducted to study three group compound enzymes toregulate the effect of diet metabolizable energy and DM digestibility by force feeding-empting method. Animal is used One-way randomized blocks experimental design.The cherry valley ducklings was conducted at 4 treatments, each treatment had4duplicates,each duplicate had 3 ducklings with male. The result suggested NSPenzyme complex improved apparent metabolizable energy and digestibility of DMin the ducklings diets(P＜0.05); And apparent metabolizable energy and digestibilityof DM had direct ratio. The effect of compound enzyme on regulation of apparentmetabolizable energy and digestibility of DM:NSP X_2＞NSP X_1＞NSPX_3.The trailtreatment B、C、D of apparent metabolizable energy and DM digestibility wasimproved 0.638MJ/kg 8.84％、0.96MJ/kg 11.27％、0.479 MJ/kg 4.04％to the contratA.
引文
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