肝应春与过敏免疫相关性的理论和实验研究
摘要
中医学“天人相应”理论认为人体五脏功能的变化与自然气候的变化是一致的,是中医学的重要理论基础。“肝应春”是“天人相应”理论中的重要部分,是指肝与春季的气候变化具有同步振荡和协同关系。中医学对于五脏功能的认识与西医学对于机体各系统的认识之间存在着很多相通的地方,这为从中西医结合的角度研究脏腑调控理论提供了必要的切入点,如中医的风邪和现代医学的过敏原就有很多相似处。本课题组在“天人相应”的研究中,已经对“心应夏”、“肾应冬”和“肺应秋”都有了一定的理论和实验研究,而“肝应春”的实验研究尚属空白。通过对中医“肝应春”的理论和实验研究,能深化对中医肝藏象本质的认识,更好的为临床服务。
1目的
本研究从现代医学过敏免疫角度阐述中医“肝应春”理论的免疫学内涵,为临床过敏性疾病的防治提供理论和实验依据。
2方法
2.1理论研究
本研究采用文献资料法和逻辑分析法,对历代关于肝应春理论进行系统梳理。
2.2实验研究
2.2.1实验动物
健康SD雄性大鼠96只,清洁级,体重150-170g,分别于2010年春分、夏至、秋分、冬至(简称二分、二至)前四十天购入。随机分为生理组、伪手术组和手术组,每组各8只。自然光照,室温饲养。手术组和伪手术组分别于二分、二至前一个月施行造模手术,与生理组在相同条件下饲养到二分、二至当日晚8：00以后处死取材。
2.2.2检测方法
血清免疫应答相关物质：IgE、IgG、TNF-α、IL-13:采用ELISA法。血清Mel：采用ELISA法。胸腺指数、脾脏指数直接称重。
2.2.3统计分析：
统计软件：SPSS11.7,数据表示：均数±标准差,检验方法：单因素方差分析(One-WayANOVA),以P<0.05有显著差异,P<0.01有极显著差异。
3结果
3.1理论研究结果
(1)肝应春理论是在天人相应理论指导下,通过取象比类为思维模式,以阴阳五行为理论框架,以气理论为肝与春联系的中介,逐步形成的一种时脏相应理论。
(2)中医“肝应春”理论的特点是：肝与春季光照相应；肝与春季风气相应。春季自然界阳气生发,风气渐盛,人体肝气顺应自然生发特点,疏泄功能旺盛,易产生内风,外风引动内风则春季多发与风邪相关的疾病(过敏性疾病等)。
(3)风邪除在肝脏所主的季节对人体造成伤害外,还在不同季节伤害相应之脏,并且常兼时令主气。如夏季风热伤心,秋季风燥伤肺,冬季风寒伤肾等,这可能是风邪致病(过敏性疾病)不同季节发作的生理病理基础。
(4)过敏性疾病属于西医范畴,但它和中医的风邪所导致的疾病一样,具有起病急,发病快,易反复发作,部位多变,游走不定和瘙痒难耐的特点。春季多风,此时也是过敏性疾病的高发季节,并且过敏原与风邪具有明显的相关性。这为临床从风论治过敏性疾病提供了理论基础。
(5)中医在临床上治疗过敏性疾病的过程中发现从肝论治和从风论治都具有明显的疗效。
3.2实验研究结果
3.2.1季节变化及摘除松果腺对免疫器官的影响
(1)胸腺指数：生理组胸腺指数具有春季>夏季>秋季>冬季的趋势,与春夏相较,秋冬具有极显著差异(P<0.01)；手术组与同一季节生理组相比,春夏呈下降趋势,秋冬呈上升趋势,春分、秋分时有显著差异(P<0.05)。
(2)脾脏指数：生理组脾脏指数具有夏季>春季>秋季>冬季的趋势,与冬至相比,春分、夏至有极显著差异(p<0.01),秋分差异显著(P<0.05)；手术组与同一季节生理组相比无显著差异,冬春略低,夏秋呈上升趋势。
3.2.2季节变化及摘除松果腺对血清免疫因子的影响
(1)血清TNF-α:生理组血清TNF-α含量具有冬季>秋季>夏季>春季的趋势,与春分和夏至相较,秋分和冬至具有极显著差异(P<0.01)；手术组与同一季节生理组相比,均无显著差异(P>0.05),但较当季生理组都有升高的趋势；三组的季节节律始终表现为：冬季>秋季>夏季>春季的趋势,且其含量冬季明显高于其他三季。
(2)血清IL-13：生理组血清IL-13含量具有冬季>夏季>秋季>春季的趋势,春分、夏至、秋分与冬至比较,均有极显著性差异(P<0.01)；手术组与同一季节生理组相比,秋分时有极显著差异(P<0.01),各季节较生理组都有升高的趋势。虽然伪手术组和手术组的血清IL-13含量季节节律有所改变,但冬季依然明显高于其他三季。
(3)血清IgG：生理组血清IgG含量具有夏季>春季>冬季>秋季的趋势,春分、秋分、冬至与夏至相比,有极显著差异(P<0.01)。秋分、冬至与春分相比,也有极显著差异(P<0.01)；手术组与同一季节生理组相比,冬至时有显著差异(P<0.05),各季节较生理组都有升高的趋势；三组的季节节律始终表现为：春夏明显高于秋冬的特点。
(4)血清IgE：生理组血清IgE含量具有冬季>春季>夏季>秋季的趋势,与春分相比,夏至、秋分有极显著差异(P<0.01),与夏至相比,秋分、冬至也有极显著差异(P<0.01),秋分和冬至相比亦有极显著差异(P<0.01)；手术组与同一季节生理组相比,秋分时差异极为显著(P<0.01),较当季生理组都有升高的趋势；三组的季节节律始终表现为：春夏明显高于秋冬的特点。
3.2.3季节变化及摘除松果腺对血清褪黑素的影响
生理组血清Mel含量具有春季>冬季>秋季>夏季的趋势,夏至、秋分与春分比较,有显著性差异(P<0.05),血清Mel春季为的含量最高；同季节手术组血清Mel含量较生理组明显降低,以春分为差异最显著(P<0.05)；手术组血清Mel四季含量并无明显差异,与生理组的节律性明显不同。
4结论
(1)现代医学的过敏免疫属于中医肝功能的范畴。运用文献整理法和逻辑分析法,以春季风气通于肝,春季为过敏性疾病的高发季节为依据,从过敏原属于中医风邪范畴的角度可推理出现代医学的过敏免疫属于中医肝功能的范畴。
(2)临床上在治疗春季高发的过敏性疾病时可从“肝”从“风”论治。通过临床上关于过敏性疾病的中医治疗和所用方药可知从内因的肝和外因的风对过敏性疾病的治疗行之有效。
(3)血清IgG、IgE可能与春季高发的过敏性疾病有关,而血清TNF-α和IL-13可能与冬季高发的过敏性疾病有关。在选择的四组过敏免疫指标中血清IgG、IgE都有春季明显升高的趋势,而血清TNF-α和IL-13则都有冬季明显升高的趋势。
(4)松果腺及褪黑素对过敏免疫具有调节作用。具体表现为松果腺对机体血清免疫因子具有抑制作用,而对免疫器官的发育具有调节作用；褪黑素对机体的免疫系统具有复杂的调节作用。
(5)中医肝应春的内涵与松果腺褪黑素及机体过敏免疫具有相关性。
In TCM theory,"Correspondence between Man and Nature" which is an important theoretical basis In traditional Chinese Medicine means man and nature is an integrated whole."Liver correspondence to Spring" which is an important part of "Correspondence between Man and Universe"Refers to the liver and the spring climate change with synchronous oscillations and collaborative relationship.there is many similarities understanding between Chinese medicine for five organs system and the respiratory system, motor system, genitourinary system, digestive system, circulatory system of Western Medicine,Such as the wind evil of traditional Chinese Medicine and the allergen of modern medicine.The combination of traditional Chinese and Western medicine research internal control theory provides a good bond and bridge.The research group of "correspondence between man and nature" in the study, has been on the "kidney correspondence to winter","lung correspondence to autumn" and "heart correspondence to summer" has certain theoretical and experimental studies, but "liver correspondence to spring" is still belongs to blank.On the theory research of "liver correspondence to spring" contributes to deepening on the understanding of liver function in seasonal changes,is important clinical guidance to occur allergic disease prevention and treatment in the spring,and deepen the understanding of the essence of the liver.
1Objective
Through literature this study found that spring is the season of high incidence of allergic disease. we Conduct the four seasons change on allergy of immunology experimental study,begin with the relationship of wind evil and allergic disease.From the allergy of immunology in modern medicine perspective to explain the essence of the traditional Chinese medicine "liver correspondence to spring".
2Methods
2.1Theoretical methods
This study which used the method of literature and logical analysis, to allergic diseases occur in the spring you as the clue, correlation of the wind evil of traditional Chinese medicine and the allergy of immunology in modern medicine as a starting point discuss liver and allergic of immune correlation.In order to find the modern medicine material basis of liver in traditional Chinese Medicine.
2.2Experimental methods
2.2.1Experimental animals
Experimental animals:twenty-four SD male rats, clean,weighted150-170g, brought forty days before vernal equinox, summer solstice, autumnal equinox and winter solstice in2010, were randomly divided into three groups:normal group, fake group and operation group (pinealectomy group), there were eight rats in every group. All rats received natural light, ate and drank freely, and were controlled under certain room temperature. Fake group and operation group are operated a month before vernal equinox, summer solstice, autumnal equinox and winter solstice. All the rats would be raised before being beheaded at8o'clock at night in vernal equinox, summer solstice, autumnal equinox and winter solstice.
2.2.2Test methods
Serum immune response related to substance lgE, lgG, TNF-A.IL-13:using ELISA method.Serum Mel:using ELISA method. The thymus index, spleen index direct weighing.
2.2.3Analysis method:
All the data were showed by mean±standard error. The differences of groups were tested by SPSS11.0. The values were calculated with Duncan's multiple range test or Student's test. P<0.05meant obvious difference.
3Results
3.1Results of theoretical study
(1)From research of "Liver correspondence to Spring" by history of Chinese medicine,it can be found that liver function will be appeared rhythmic changes in the spring,thus affecting the whole human body function. Deep understanding of liver function of rhythm characteristics have the profound significance for ome seasonal incidence of disease prevention and treatment.(2)Allergic diseases belongs to the category of Western medicine,but it has a rapid onset, rapid onset, easy to repeated attacks, parts move uncertain and changeable, itching intolerable characteristics,the same of Chinese medicine diseases caused by pathogenic wind.(3)The onset of allergic disorders is allergen invasion causes the allergic immune response process, while the majority of allergic diseases predominate in the allergic immune index IgE.4.Chinese medicine in clinical treatment of allergic diseases were found during treatment from liver and dealing with the wind has apparent curative effect.
3.2Results of experiments
3.2.1Influence on immune organs by seasonal variation and extracting pinealectomy
(1)Thymus index:Physiological group had trend spring>summer>autumn>winter, there was a very significant difference (P<0.01) in autumn and winter compared with in spring and summer;In the same season,there was a significant difference (P<0.05) and showing the trend of content descending in spring and summer and rising in autumn and winter that operation group compared with physiology group.
(2)Spleen index:Physiological group had the trend of content summer>spring>autumn>winter,there was a very significant difference (P<0.01) in spring and summer compared with in winter,there was a significant difference (P<0.05) in autumn compared with in winter;In the same season,there was no significant difference but showing the trend of content descending in spring and winter and rising in autumn and summer that operation group compared with physiology group.
3.2.2Influence on immune factors in serum by seasonal variation and extracting pinealectomy
(1) TNF-α in Serum:Physiological group had the trend of content winter>autumn>summer>spring, there was a very significant difference (P<0.01) in autumn and winter compared with in spring and summer;In the same season,there was no significant difference but showing the the trend of content rising that operation group compared with physiology group.Three groups of seasonal rhythm always embodied the trend winter>autumn>summer>spring,and the content was highest in winter compared with other season.
(2)IL-13in Serum:Physiological group had the trend of content winter>summer>autumn>spring, there was a very significant difference (P<0.01) in spring and summer and autumn compared with in winter;In the same season,there was a very significant difference (P<0.01) in autumn and showing the the trend of content rising that operation group compared with physiology group.Although the seasonal rhythm of the content IL-13of pseudo operation group and operation group was changed,the content was highest in winter compared with other season.
(3)IgG in Serum:Physiological group had the trend of content summer>spring>winter>autumn, there was a very significant difference (P<0.01) in spring and autumn and winter compared with in summer;ln the same season,there was a significant difference (P<0.05) in winter and showing the the trend of content rising that operation group compared with physiology group;Three groups of seasonal rhythm was always embodied in spring and summer higher than in autumn and winter.
(4)IgE in Serum:Physiological group had the trend of content winter>spring>summer>autumn,there was a very significant difference (P<0.01) in summer and autumn compared with in spring,and was a very significant difference (P<0.01) in autumn and winter compared with in summer,and was a very significant difference (P<0.01) in autumn compared with in winter; In the same season,there was a very significant difference (P<0.01) in autumn and showing the the trend of content rising that operation group compared with physiology group;Three groups of seasonal rhythm was always embodied in spring and summer higher than in autumn and winter.
3.2.2Influence on by seasonal variation and extracting pinealectomy
Mel in serum of Physiological group had the trend of content winter>spring>summer>autumn,there was a significant difference(P<0.05)in autumn compared with in spring,and the content in spring is highest than other season; In the same season,there was a significant difference (P<0.05) in spring and showing the the trend of content descending that operation group compared with physiology group;Operation group Mel in serum content did not differ significantly between seasons, and physiological group rhythm was significantly different.
4Conclusions
(1)According to "Literature review method" and "Logical analysis" and allergen belonging to the Chinese wind evil category,by spring high incidence of allergic disease as carrier,we can deduce that The modern medicine allergic immune belongs to the traditional Chinese medicine liver function category.
(2)In clinical we can cure allergic diseases which is high incidence in spring. from the liver and the wind.
(3)In the six index of allergy and Immunology,serum IgG and IgE, thymus and spleen index has increased in spring,so the four index are closely related with the allergic diseases which is high incidence in spring.TNF-a in Serum and IL-13in Serum are closely related with the allergic diseases which is high incidence in winter.
(4)The pineal and Mel has a modulatory role on allergen immunotherapy,The specific performance is that the pineal have inhibition to serum immune factors, and have complex regulatory role to the immune organs.Mel has a complex regulatory role to the body's immune system.
(5)The connotation of "Liver correspondence to Spring" is related to the pineal and Mel and Allergic immune of body
1目的
本研究从现代医学过敏免疫角度阐述中医“肝应春”理论的免疫学内涵,为临床过敏性疾病的防治提供理论和实验依据。
2方法
2.1理论研究
本研究采用文献资料法和逻辑分析法,对历代关于肝应春理论进行系统梳理。
2.2实验研究
2.2.1实验动物
健康SD雄性大鼠96只,清洁级,体重150-170g,分别于2010年春分、夏至、秋分、冬至(简称二分、二至)前四十天购入。随机分为生理组、伪手术组和手术组,每组各8只。自然光照,室温饲养。手术组和伪手术组分别于二分、二至前一个月施行造模手术,与生理组在相同条件下饲养到二分、二至当日晚8：00以后处死取材。
2.2.2检测方法
血清免疫应答相关物质：IgE、IgG、TNF-α、IL-13:采用ELISA法。血清Mel：采用ELISA法。胸腺指数、脾脏指数直接称重。
2.2.3统计分析：
统计软件：SPSS11.7,数据表示：均数±标准差,检验方法：单因素方差分析(One-WayANOVA),以P<0.05有显著差异,P<0.01有极显著差异。
3结果
3.1理论研究结果
(1)肝应春理论是在天人相应理论指导下,通过取象比类为思维模式,以阴阳五行为理论框架,以气理论为肝与春联系的中介,逐步形成的一种时脏相应理论。
(2)中医“肝应春”理论的特点是：肝与春季光照相应；肝与春季风气相应。春季自然界阳气生发,风气渐盛,人体肝气顺应自然生发特点,疏泄功能旺盛,易产生内风,外风引动内风则春季多发与风邪相关的疾病(过敏性疾病等)。
(3)风邪除在肝脏所主的季节对人体造成伤害外,还在不同季节伤害相应之脏,并且常兼时令主气。如夏季风热伤心,秋季风燥伤肺,冬季风寒伤肾等,这可能是风邪致病(过敏性疾病)不同季节发作的生理病理基础。
(4)过敏性疾病属于西医范畴,但它和中医的风邪所导致的疾病一样,具有起病急,发病快,易反复发作,部位多变,游走不定和瘙痒难耐的特点。春季多风,此时也是过敏性疾病的高发季节,并且过敏原与风邪具有明显的相关性。这为临床从风论治过敏性疾病提供了理论基础。
(5)中医在临床上治疗过敏性疾病的过程中发现从肝论治和从风论治都具有明显的疗效。
3.2实验研究结果
3.2.1季节变化及摘除松果腺对免疫器官的影响
(1)胸腺指数：生理组胸腺指数具有春季>夏季>秋季>冬季的趋势,与春夏相较,秋冬具有极显著差异(P<0.01)；手术组与同一季节生理组相比,春夏呈下降趋势,秋冬呈上升趋势,春分、秋分时有显著差异(P<0.05)。
(2)脾脏指数：生理组脾脏指数具有夏季>春季>秋季>冬季的趋势,与冬至相比,春分、夏至有极显著差异(p<0.01),秋分差异显著(P<0.05)；手术组与同一季节生理组相比无显著差异,冬春略低,夏秋呈上升趋势。
3.2.2季节变化及摘除松果腺对血清免疫因子的影响
(1)血清TNF-α:生理组血清TNF-α含量具有冬季>秋季>夏季>春季的趋势,与春分和夏至相较,秋分和冬至具有极显著差异(P<0.01)；手术组与同一季节生理组相比,均无显著差异(P>0.05),但较当季生理组都有升高的趋势；三组的季节节律始终表现为：冬季>秋季>夏季>春季的趋势,且其含量冬季明显高于其他三季。
(2)血清IL-13：生理组血清IL-13含量具有冬季>夏季>秋季>春季的趋势,春分、夏至、秋分与冬至比较,均有极显著性差异(P<0.01)；手术组与同一季节生理组相比,秋分时有极显著差异(P<0.01),各季节较生理组都有升高的趋势。虽然伪手术组和手术组的血清IL-13含量季节节律有所改变,但冬季依然明显高于其他三季。
(3)血清IgG：生理组血清IgG含量具有夏季>春季>冬季>秋季的趋势,春分、秋分、冬至与夏至相比,有极显著差异(P<0.01)。秋分、冬至与春分相比,也有极显著差异(P<0.01)；手术组与同一季节生理组相比,冬至时有显著差异(P<0.05),各季节较生理组都有升高的趋势；三组的季节节律始终表现为：春夏明显高于秋冬的特点。
(4)血清IgE：生理组血清IgE含量具有冬季>春季>夏季>秋季的趋势,与春分相比,夏至、秋分有极显著差异(P<0.01),与夏至相比,秋分、冬至也有极显著差异(P<0.01),秋分和冬至相比亦有极显著差异(P<0.01)；手术组与同一季节生理组相比,秋分时差异极为显著(P<0.01),较当季生理组都有升高的趋势；三组的季节节律始终表现为：春夏明显高于秋冬的特点。
3.2.3季节变化及摘除松果腺对血清褪黑素的影响
生理组血清Mel含量具有春季>冬季>秋季>夏季的趋势,夏至、秋分与春分比较,有显著性差异(P<0.05),血清Mel春季为的含量最高；同季节手术组血清Mel含量较生理组明显降低,以春分为差异最显著(P<0.05)；手术组血清Mel四季含量并无明显差异,与生理组的节律性明显不同。
4结论
(1)现代医学的过敏免疫属于中医肝功能的范畴。运用文献整理法和逻辑分析法,以春季风气通于肝,春季为过敏性疾病的高发季节为依据,从过敏原属于中医风邪范畴的角度可推理出现代医学的过敏免疫属于中医肝功能的范畴。
(2)临床上在治疗春季高发的过敏性疾病时可从“肝”从“风”论治。通过临床上关于过敏性疾病的中医治疗和所用方药可知从内因的肝和外因的风对过敏性疾病的治疗行之有效。
(3)血清IgG、IgE可能与春季高发的过敏性疾病有关,而血清TNF-α和IL-13可能与冬季高发的过敏性疾病有关。在选择的四组过敏免疫指标中血清IgG、IgE都有春季明显升高的趋势,而血清TNF-α和IL-13则都有冬季明显升高的趋势。
(4)松果腺及褪黑素对过敏免疫具有调节作用。具体表现为松果腺对机体血清免疫因子具有抑制作用,而对免疫器官的发育具有调节作用；褪黑素对机体的免疫系统具有复杂的调节作用。
(5)中医肝应春的内涵与松果腺褪黑素及机体过敏免疫具有相关性。
In TCM theory,"Correspondence between Man and Nature" which is an important theoretical basis In traditional Chinese Medicine means man and nature is an integrated whole."Liver correspondence to Spring" which is an important part of "Correspondence between Man and Universe"Refers to the liver and the spring climate change with synchronous oscillations and collaborative relationship.there is many similarities understanding between Chinese medicine for five organs system and the respiratory system, motor system, genitourinary system, digestive system, circulatory system of Western Medicine,Such as the wind evil of traditional Chinese Medicine and the allergen of modern medicine.The combination of traditional Chinese and Western medicine research internal control theory provides a good bond and bridge.The research group of "correspondence between man and nature" in the study, has been on the "kidney correspondence to winter","lung correspondence to autumn" and "heart correspondence to summer" has certain theoretical and experimental studies, but "liver correspondence to spring" is still belongs to blank.On the theory research of "liver correspondence to spring" contributes to deepening on the understanding of liver function in seasonal changes,is important clinical guidance to occur allergic disease prevention and treatment in the spring,and deepen the understanding of the essence of the liver.
1Objective
Through literature this study found that spring is the season of high incidence of allergic disease. we Conduct the four seasons change on allergy of immunology experimental study,begin with the relationship of wind evil and allergic disease.From the allergy of immunology in modern medicine perspective to explain the essence of the traditional Chinese medicine "liver correspondence to spring".
2Methods
2.1Theoretical methods
This study which used the method of literature and logical analysis, to allergic diseases occur in the spring you as the clue, correlation of the wind evil of traditional Chinese medicine and the allergy of immunology in modern medicine as a starting point discuss liver and allergic of immune correlation.In order to find the modern medicine material basis of liver in traditional Chinese Medicine.
2.2Experimental methods
2.2.1Experimental animals
Experimental animals:twenty-four SD male rats, clean,weighted150-170g, brought forty days before vernal equinox, summer solstice, autumnal equinox and winter solstice in2010, were randomly divided into three groups:normal group, fake group and operation group (pinealectomy group), there were eight rats in every group. All rats received natural light, ate and drank freely, and were controlled under certain room temperature. Fake group and operation group are operated a month before vernal equinox, summer solstice, autumnal equinox and winter solstice. All the rats would be raised before being beheaded at8o'clock at night in vernal equinox, summer solstice, autumnal equinox and winter solstice.
2.2.2Test methods
Serum immune response related to substance lgE, lgG, TNF-A.IL-13:using ELISA method.Serum Mel:using ELISA method. The thymus index, spleen index direct weighing.
2.2.3Analysis method:
All the data were showed by mean±standard error. The differences of groups were tested by SPSS11.0. The values were calculated with Duncan's multiple range test or Student's test. P<0.05meant obvious difference.
3Results
3.1Results of theoretical study
(1)From research of "Liver correspondence to Spring" by history of Chinese medicine,it can be found that liver function will be appeared rhythmic changes in the spring,thus affecting the whole human body function. Deep understanding of liver function of rhythm characteristics have the profound significance for ome seasonal incidence of disease prevention and treatment.(2)Allergic diseases belongs to the category of Western medicine,but it has a rapid onset, rapid onset, easy to repeated attacks, parts move uncertain and changeable, itching intolerable characteristics,the same of Chinese medicine diseases caused by pathogenic wind.(3)The onset of allergic disorders is allergen invasion causes the allergic immune response process, while the majority of allergic diseases predominate in the allergic immune index IgE.4.Chinese medicine in clinical treatment of allergic diseases were found during treatment from liver and dealing with the wind has apparent curative effect.
3.2Results of experiments
3.2.1Influence on immune organs by seasonal variation and extracting pinealectomy
(1)Thymus index:Physiological group had trend spring>summer>autumn>winter, there was a very significant difference (P<0.01) in autumn and winter compared with in spring and summer;In the same season,there was a significant difference (P<0.05) and showing the trend of content descending in spring and summer and rising in autumn and winter that operation group compared with physiology group.
(2)Spleen index:Physiological group had the trend of content summer>spring>autumn>winter,there was a very significant difference (P<0.01) in spring and summer compared with in winter,there was a significant difference (P<0.05) in autumn compared with in winter;In the same season,there was no significant difference but showing the trend of content descending in spring and winter and rising in autumn and summer that operation group compared with physiology group.
3.2.2Influence on immune factors in serum by seasonal variation and extracting pinealectomy
(1) TNF-α in Serum:Physiological group had the trend of content winter>autumn>summer>spring, there was a very significant difference (P<0.01) in autumn and winter compared with in spring and summer;In the same season,there was no significant difference but showing the the trend of content rising that operation group compared with physiology group.Three groups of seasonal rhythm always embodied the trend winter>autumn>summer>spring,and the content was highest in winter compared with other season.
(2)IL-13in Serum:Physiological group had the trend of content winter>summer>autumn>spring, there was a very significant difference (P<0.01) in spring and summer and autumn compared with in winter;In the same season,there was a very significant difference (P<0.01) in autumn and showing the the trend of content rising that operation group compared with physiology group.Although the seasonal rhythm of the content IL-13of pseudo operation group and operation group was changed,the content was highest in winter compared with other season.
(3)IgG in Serum:Physiological group had the trend of content summer>spring>winter>autumn, there was a very significant difference (P<0.01) in spring and autumn and winter compared with in summer;ln the same season,there was a significant difference (P<0.05) in winter and showing the the trend of content rising that operation group compared with physiology group;Three groups of seasonal rhythm was always embodied in spring and summer higher than in autumn and winter.
(4)IgE in Serum:Physiological group had the trend of content winter>spring>summer>autumn,there was a very significant difference (P<0.01) in summer and autumn compared with in spring,and was a very significant difference (P<0.01) in autumn and winter compared with in summer,and was a very significant difference (P<0.01) in autumn compared with in winter; In the same season,there was a very significant difference (P<0.01) in autumn and showing the the trend of content rising that operation group compared with physiology group;Three groups of seasonal rhythm was always embodied in spring and summer higher than in autumn and winter.
3.2.2Influence on by seasonal variation and extracting pinealectomy
Mel in serum of Physiological group had the trend of content winter>spring>summer>autumn,there was a significant difference(P<0.05)in autumn compared with in spring,and the content in spring is highest than other season; In the same season,there was a significant difference (P<0.05) in spring and showing the the trend of content descending that operation group compared with physiology group;Operation group Mel in serum content did not differ significantly between seasons, and physiological group rhythm was significantly different.
4Conclusions
(1)According to "Literature review method" and "Logical analysis" and allergen belonging to the Chinese wind evil category,by spring high incidence of allergic disease as carrier,we can deduce that The modern medicine allergic immune belongs to the traditional Chinese medicine liver function category.
(2)In clinical we can cure allergic diseases which is high incidence in spring. from the liver and the wind.
(3)In the six index of allergy and Immunology,serum IgG and IgE, thymus and spleen index has increased in spring,so the four index are closely related with the allergic diseases which is high incidence in spring.TNF-a in Serum and IL-13in Serum are closely related with the allergic diseases which is high incidence in winter.
(4)The pineal and Mel has a modulatory role on allergen immunotherapy,The specific performance is that the pineal have inhibition to serum immune factors, and have complex regulatory role to the immune organs.Mel has a complex regulatory role to the body's immune system.
(5)The connotation of "Liver correspondence to Spring" is related to the pineal and Mel and Allergic immune of body
引文
[1]柳宝诒.柳选四家医案[M].北京：中国中医药出版社,1997：51.
[2]陈颖之,赵仓焕.刍议“肝旺于春”[J].贵阳中医学院学报,2009,31(5)：44-45.
[3]杨云霜.基于五脏应时发病基础的气温骤变诱发脑卒中机制的研究[J].北京中医药大学博士论文,2009.
[4]陈玉萍,马淑然,王庆国,赵燕.中医“肝应春”理论内涵的探讨[J].中华中医药杂志,2011,26(5)：1172-1175.
[5]袁卫玲,史楠楠,郭霞珍.过敏性疾患季节易感性与“肝应春”理论相关性探讨[J].中国中医药信息杂志,2011,18(4)：90-91.
[6]王玉芳.肝主升发与生发[J].中医药信息,2010.27(4)：6-7.
[7]肖昌湖,李鲁宁.季节指数预测法在精神病医院管理中的应用[J].赣南医学院学 报,2005,25(5)：610-611.
[8]郭玉秀.情感性精神障碍发病高峰时间与季节关系的圆形分布统计分析[J].中国民康医学,2004,11：662-663.
[9]郭永贵,郭玉秀,张衍军,等.情感性精神障碍患者发病高峰时间与季节关系的圆形分布统计分析[J].中国民康医学,2006,18(9)：745-746.
[10]张云,戴福强,唐怀瑞.情感性精神障碍首次发病与季节和气候的关系分析[J].临床中老年保健,2001,4(2)：85-86.
[11]代福强,陆万美,朱文波.231例情感性精神障碍的复发因素分析[J].四川精神卫生,2002,15(2)：89-91.
[12]金光亮,梁怡,郭霞珍等.有关抑郁症季节性发病机理的研究及其启示[J].北京中医药大学学报,1997,20(1)：15-16.
[13]徐砚通,郭霞珍.“时藏相应”生理机制实验研究[J].中国中医基础医学杂志,2001,7(5)：16-18.
[14]Sakamoto K, Kamo T, Nakadaira S, et al. An ation wide survey of seasonal affective disorder at 53 outpatientuniversity clinics in Japan[J].Acta Psychiatr Scand,1993,87: 258-265.
[15]David S.Schlager.Early-Morning Administration of Short-Acting β Blockers for Treatment of Winter Depress[J].Am J Psychiatry,1994,151 (9):1383-1385.
[16]顾中范.褪黑激素与季节性情感性精神病[J].上海精神医学,1990,2(4)：206.
[17]In-Young Y, Denirl FK.. Luteinizing hormonr following light exposure in healthy young men [J]. Neuroscience Letters,2003,341:25-28.
[18]吴岳峰,高成阁.引起精神分裂症患者出生的季节性差异因素[J].国外医学医学地理杂志,2006,27(3)：137-141.
[19]工海成,刘庆武,张敏.分裂样精神病发病与季节节气关系的序列分析[J].郴州医学高等专科学校学报,2003,5(3)：10-12.
[20]吴燕燕,乔振刚.“肝木主病”说指导春季临床治疗[J].光明中医杂志,1995,(6)：5-6.
[21]赵满华.时间医学验案选例[J].辽宁中医杂志,1983,7(4)：32-34.
[22]任兰民,李志华.精神分裂症的发病季节性调查.预防医学文献信息,2000,6(4)：345.
[23]计庆明、顾世芬等.精神疾病与气象要素相关性研究.山东精神医学,2003,16(2)：65—67.
[24]陈红.3037例精神分裂症出生与入院季节的关系分析.中国民康医学杂志,2004,16(5)：271-272.
[1]林颖,陈达灿.变态反应性疾病发病相关因素的研究进展[J].中国中西医结合皮肤性病学杂志,2005,4(1)：60-63
[2]谈欧,王德钧.韩树人教授淡“哮喘专主于风”[J].南京中医药大学学报,2007,23(4)：259.
[3]宋苹“过敏煎”联合开瑞坦治疗过敏性咳嗽30例[J].实用医学杂志.2009,25(1)：164.
[4]李庆生.过敏性疾病应重视病证结合从“风”论治用药[J].云南中医中药杂 志,1998,19(3)：9.[5]栗庆林.从肝从风论治支气管哮喘[J].光明中医,2004,19(6)：18.
[6]翟文生,朱珊.过敏性咳嗽从肝论治的临床研究[J].中国医药学报,2003,18(2)：123.
[7]范瑞强,廖元兴.中西医结合临床皮肤性病学[M].广州：世界图书出版公司,2003,462.
[8]李元文,张丰川,周德瑛.辨证治疗特应性皮炎35例[J].北京中医药大学学报,2002,25(5)：69-70.
[9]余土根.中西医结合诊治特应性皮炎的思路[J].浙江中西医结合杂志,2005,15(8)：463-464.
[10]李彦军,刘澈.马淑然教授运用加味过敏煎治疗过敏性疾病验案举隅.中国中医药信息杂志.2010,7(7)：89-90.
[11]马群力.消风散治疗过敏性疾病验案5则[J].现代中西医结合杂志,2004,13(21)：2874-2875.
[12]程丑夫.过敏性疾病当从风论治[J].江苏中医药,2002,23(10)：1-3.
[13]毕莲.傅汝林治疗过敏性紫瘫经验[J].辽宁中医杂志,2009,36(3)：345-346.
[14]尹璐.尹铁汉治疗过敏性疚病的经验叮[J]医学创新研究,2006,]2(3)：12.
[15]张谈.张迪蛟治疗过敏性疾病验案四则[J].江西中医药,2005,36(271)：9-10.
[16]陈斌.自拟消风饮治疗过敏性疾病30例[J]Chinese Journal of the Practieal Chinese with Modem Medieine,2005,VOL.(18)NO.23.
[17]林蔚达.试述过敏性鼻炎的中医辨治[J].浙江中医杂志,2009,6(44)：455.
[18]Vandenbulcke L, Bachert C, Van Cauwenberge P, et al. The innate immune system and its role in allergic disorders[J]. Int Arch Allergy Immunol,2006,139 (2):159-165.
[19]Renz H,Smith H R, Henson J E,et al. Aerosolized antigen exposure without adjuvant causes increased IgE production and increased airway responsiveness in the mouse[J]. J Allergy Clin Immunol,1992,89(6):1127-1138.
[20]陈木开,廖绮曼,林志斌.遗传过敏性皮炎血清特异性IgE分析[J].中国皮肤性病学杂志,2000,14：151-152.
[21]庄丽华.吸入性变应原诱导的皮肤迟发相反应[J].国外医学,皮肤性病学分册,1995,21：328-331.
[22]刘彦群,赵辨.季节性面部接触性皮炎致病因素及其发病机理的初步探讨[J].中华皮肤科杂志,1992,25(4)：224.
[23]庄丽华,赵辨.43例季节性接触性皮炎皮肤实验研究[J].中华皮肤科杂志,1996,29(1)：18-21.
[24]严道金,赵辨.季节性接触性皮炎花粉斑贴实验阳性部位皮肤IgE检测[J].临床皮肤科杂志,1998,31(4)：250.
[25]赵辨.临床皮肤病学[M].3版.南京：江苏科学技术出版社,2001：63.
[26]程少为,刘春玲,兰东,等.荨麻疹患者特异性IgER及过敏原检测与分析[J].中华皮肤科杂志,2003,36(11)：637-639.
[27]张金桃,陈瑾萍,罗佩谊等.特应性皮炎和慢性荨麻疹儿童IgE检测及变应原分析[J].广东医学杂志,2007,28(1())：1688-1690.
[28]李林峰.接触性皮炎与皮肤变态反应[M].2版.北京:北京大学医学出版社,2003：201.
[29]李明华,殷凯生,朱栓立.哮喘病学[M].北京：人民卫生出版社,1998：47.
[30]李明华,殷凯生,朱栓立.哮喘病学[M].北京：人民卫生出版社,1998：113.
[31]MacGlashan DW, Bochner BS, Adelmen DC, et al.Down-regulation of Fc ε R Ⅰ expression on human basophils duringin vivotreatment of atopic patients with anti-IgE antibody[J].J Immunol,1997,158(3):1438.
[32]MacGlashan DW, Mckenzie-White J, Chichester K, et al.In vitro regulation of Fc ε R Ⅰ expression on human basophils by IgE antibody [J]. Blood,1998,91(5):1633.
[33]Bang LM, Plosker GL. Spotlight on omalizumab in aller-gic asthma[J]. Biol Drugs, 2004,18(6):415.
[34]Hvatum M, Scott H, Brandtzaeg P. Serum IgG subclass antibodies to a variety of food antigens in patients with coeliac disease [J]. Gut,1992,33(5):628632.
[35]王福新,陈丹绿,周世良等.变态反应疾病血清IgE和IgG分析[J].江西医学院学报,2006,46(1)：183.
[36]张利,赖蓉,尹洁.湿疹患者食物过敏原特异性IgG检测及意义[J].四川医学,2009,30(2)：256-257.
[37]De Waal Malefyt R, Figdor CG, Huijkens R,et al. Effects of IL-13 on phenotype, cytokine production and cytotoxic function of human monocyte. Comparison with IL-4 and modulation by IFN-γ or IL-10[J]. J Immunol,2000,158:6370-6381.
[38]Briere F, Bridon JM, Servet C,et al.IL-10 and IL-13 as B cell growth and differentiation factors[J]. Nouv Rev Fr Hematol,2000,40(3):233-235.
[39]Kornmann M, Kleeff J, Debinski W,et al. Pancreatic cancer cells express interkeukin-13 and-4 receptors, and their growth is inhibited by pseudomonas exotoxin coupled to interleukin-13 and-4[J]. Anticancer Res,1999,19:125-131.
[40]De Waal Malefyt R, Figdor CG, Richard H, et al. Effecte of IL-13 on phenotype, cytokine production, and cytotosin function of human monocytes:comparison with IL-4 and modulation by IFR-r or IL-10. J Immunol,1993;151(11):6370.
[41]Tengvall-Linder M,et al.Clin-Exp-Allergy,1996;26(11):1286-97.
[42]Ying S,et al.J immunol,1997;158(10):5050-7.
[43]Burrows B, Sears MR, Flannery EM,et al. Relation of the course of bronchial responsiveness from age 9 to age 15 to allergy [J]. Am J Respir Crit Care Med,1995,152(4 Pt 1):1302-1308.
[44]Saha SK, Berry MA, Parker D, et al. Increased sputum and bron-chial biopsy IL - 13 expression in severe asthma[J]. J Allergy ClinImmunol.2008,121 (3):685-691.
[45]Zhang Y, et al.J-Exp-Med,1996; 184(4):1507-12.
[46]Kotsimbos TC, et al.Proe-Asso-Am-Physicians,1996;108(5):368-73.
[47]Nasser J. et al.Am-J-Respir-Crit-Care-Med,1997; 155(3):845-51.
[48]Zhu Z, Zheng T, Homer RJ, et al.Acidic mammalian chitinase in asthmatic Th2 inflammation and IL - 13 pathway activation[J]. Sci-ence,2004,304(5677):1678 1682.
[49]Corren J. Lemanske RF Jr, et al.Lebrikizumab treatment in adults with asthma[J]. N Engl J Med,2011,[Epub ahead of print].
[50]Diaz-Sanchez D.et al.J-Allergy-Clin-immunol,1996;98(1):114-23.
[51]Pawankar RU,et al.Am-J-Respir-Crit-Care-Med,1995;152(6pt1):2059-67.
[52]Ghaffar O,et al.Am-J-Respir-Crit-Care-Med,1995;152(6pt1):2059-67.
[1]Chen Q, Wei W. Effects and mechanisms of melatonin on inflammatory and immune responses of adjuvant arthritis rat[J].Int Immunopharmacol,2002,2(10):1443-1449.
[2]Provinciali M, Di Stefano G, Bulian D, Tibaldi A,Fabrs N. Effect of melatonin and pineal grafting on thymocyte apoptosis in aging mice[J].Mech Aging Dev,1996,90(1):1-19.
[3]Dubocovich ML, Markowska M. FunctionalMT1 and MT2 melaton in receptors in mammals[J].Endocrine,2005,27(2):101-110.
[4]Oner H, Kus I, Oner J, et al. Possibleeffects of melatonin on thymus gland after pinealectomy in rats[J]. Neuro EndocrinolLett,2004,25(1-2):115-118
[5]Singh SS, Haldar C, RaiS. Melatonin and differential effectofL-thyroxine on immune system of Indian tropicalbird Perdicula asiatica[J]. Gen Comp Endocrino,1 2006,145(3): 215-221
[6]Champney TH, Prado J, Youngblood T, et, al. Immune responsiveness of splenocytes after chronic daily melatonin administration in male Syrian hamsters[J].Immunol Lett,1997, 58(2):95-100.
[7]徐峰,李经才,杨迎暴,等.褪黑素对光照制度改变影响的对抗作用[J].中国药理学通报,1994,(10)：448.
[8]秦承德.生理科学研究进展[M].沈阳：辽沈书社,1993,123-124.
[9]Arias J, Melean E,Valero N, et al. Effect of melatonin on lymphocyte proliferation and production of interleukin-2(IL-2) and interleukin-1 beta(IL-1beta) in mice splenocytes[J]. Invest Clin,2003,44(1):40-50.
[10]Maestroni GJ, Conti A. Melatonin and the immune-hematopoietic system therapeutic and adverse pharmacological correlates! JJ.Neuroimmunomodula tion,1996,3(6) 325.
[11]成令忠,钟翠平,蔡文琴.现代组织学[M].上海：上海科学技术文献出版社,2003,580.
[12]刘爱国,杨默,李志光,等.褪黑素对照射后小鼠血小板造血的保护作用[J].中华放射医学与防护杂志,2005,25(6)：538.
[13]Lissoni P, Mandala M, Rossini F, et al. (irowth factors:thrombopoietic property of the pineal hormone melatonin[J]. Hematology,1999,4(4):335-343.
[14]Rapozzi V, Zorzet S, Comelli M, Mavelli 1, Perissin L, Giraldi T. Melatonin decreases bone marrow and lymphatic toxicity of adriamycin in mice bearing TLX5 lymphoma[J]. Life Sci,1998,63(19):1701-1711.
[15]Drazen DL, Jasnow AM, Nelson RJ, Demas GE. Exposure to short days, but not short term melatonin, enhances humoral immunity of male Syrian hamsters (Mesocricetus auratus)[J],Pineal Res,2002,33(2):118-120.
[16]Shaji AV, Kulkarni SK, Agrewala JN. Regulation of secretion of IL-4 and IgG lisotype by melatonin stimulated ovalbumin specific T cells[J]. Clin Exp Immunol,1998,111(1):181-185.
[17]徐峰,李经才,杨迎暴,等.褪黑素对光照制度改变影响的对抗作用[J].中国药理学通报,1994,(10)：448.
[18]秦承德.生理科学研究进展[M].沈阳：辽沈书社,1993,123-124.
[19]Arias J, Melean E,Valero N, et al. Effect of melatonin on lymphocyte proliferation and production of interleukin-2(IL-2) and interleukin-1 beta(IL-lbeta) in mice splenocytes[J]. Invest Clin,2003,44(1):40-50.
[20]Drazen DL, Jasnow AM, Nelson RJ, Demas GE. Exposure to short days, but not short term melatonin, enhances humoral immunity of male Syrian hamsters (Mesocricetus auratus)[J],Pineal Res,2002,33(2):118-120.
[21]Shaji AV, Kulkarni SK, Agrewala JN. Regulation of secretion of IL-4 and IgG lisotype by melatonin stimulated ovalbumin specific T cells[J]. Clin Exp Immunol,1998,111(1):181-185.
[22]VigoreL, Messina G, Brivio F, et al.Psychoneuroendocrine modulation of regulatory T lymphocyte system:in vivo and in vitro effects of the pineal immunomodulating hormone melatonin[J]. In Vivo,2010,24(5):787-789.
[23]何维.医学免疫学[M].人民卫生出版社,2005,157.
[24]Wichmann MW, Zellweger R, DeMaso, Ayala A, et, al. Melatonin admini strati on attenuates depressed immune functions trauma hemorrhage[J].Surg Res,1996,63(1):256-262.
[25]LissoniP, Rovelli F, Brivio F.Circadian secretion of IL-2, IL-12,IL-6 and IL-10 in relation to the light/dark rhythm of the pineal hormone melatonin in healthy humans[J]. Nat Immun,1998,16(1):1-5.
[26]Sharman KG, Sharman EH, Yang E, et al. Dietary melatonin selectively reverses age related changes in cortical cytokine mRNA levels, and their responses to an inflamematory stimulus [J]. Neurobiol Aging,2002,23 (4):633-638.
[27]迟素敏.内分泌生理学[M].西安：第四军医大学出版社,2006,143-144.
[28]Moore CB, SiopesTD. Melatonin enhances cellular and humoral im-mune responses in the Japanese quail(Coturnix coturnix japonica)via an opiatergic mechanism[J].Gen Comp Endocrinol,2003,131(3):258-263.
[29]黄颖苏.松果腺褪黑素对下丘脑-垂体-肾上腺轴的调节作用[J].国外医学分册：内分泌代谢杂志,2001,21(2)：88.
[30]魏伟.褪黑素对炎症免疫和痛反应的作用及其机制[J].安徽医科大学学报.1999,(34)2：159-160
[31]Bonilla E, Valero N, Chacin-Bonilla L,et al. Melatonin and viral infections[J].JPinealRes, 2004,36(2):73-79.
[32]Carrillo-VicoA, LardonePJ, NajiL,et al. Beneficial pleiotropic actions of melatonin in an experimental model of septic shock in miceregulation of pro-/anti-inflammatory cytokine network, protection against oxidative damage and anti-apoptotic effects[J].JPinealRes,2005, 39(4):400-408.
[33]ImamogluM, Cay A, Cobanoglu U,et al. Effects of melatonin on suppression of renal scarring in experimental model of pyelonephritis[J].Urology,2006,67(6):1315-1319.
[34]Maestroni GJ.The immunotherapeutic potential of melationin[J]. Expert Opin Investig Drugs,2001,10:467
[35]imenez-CalianiAJ, Jimenez-Jorge S, Molinero P,et al. Dual effect of melatonin as proinflammatory and antioxidant in collagen-induced arthritis in rats[J].JPinealRes,2005, 38(2):93-99.
[36]Haase CG, TinnefeldM, Faustmann PM. The influence of immuno-modulation on psycho-neuroimmuno logical functions in benign multiple sclerosis[J].Neuroimmuno modulation,2004,11(6):365-372.
[37]HaaseCG, Faustmann PM. Benignmultiple sclerosis is characterized by a stable neuroimmuno logic network[J].Neuroimmuno modulation,2004,11(4):273-277.
[38]Jimenez-Caliani AJ, Jimenez-Jorge S, Molinero P,et al. Sex-de-pendent effect of melatonin on systemic erythematosus lupus developed inMrl/Mpj-Faslprmice:it ameliorates the disease course in females, whereas it exacerbates it in males[J].Endocrinology, 2006,147(4):1717-1724.
[39]Maestroni GJ.The photoperiod transducer melatonin and the immune hematopoietic system[J].J Photochem Photobiol B,1998,43:186.
[40]Teplitzky SR,kiefer TL,Cheng Q,et al.Chemoprevention of NMU-induced rat mammary carcinoma with the combination of melatonion of melatonion and 9-cis-retinoic acid[J].Cancer Lett,2001.168:155.
[41]何绍雄.时间药理学与时间治疗学[M].天津：天津科学技术出版社,1994,40.
[1]刘兆琳,侯慎英.赵立新.过敏煎治疗变应性鼻炎90例[J].河北中医2005,27(3)：227.
[2]朱正民,耿以安.陈虹.小青龙汤加减治疗AR[J].湖北中医杂志,2004,26(5)：38-39.
[3]张兴学.玉屏风散加减治疗AR63例[J].现代中西医结合杂志,2005,14(4)：493.
[4]吴朝晖.蔡氏抗敏鼻炎方治疗AR 35例[J].安徽中医临床杂志,2003,15(6)：469.
[5]葛荫茹,郑薇.鼻鼽中医治疗79例体会[J].当代医学,2007,(9)：92-93.
[6]苏清学.补中益气汤新用[J].中国民间疗法,.2007,1 5(1)：33-34.
[7]魏萍,魏棠,肖风英.益气通窍汤治疗AR30例[J].中国民间疗法,2006,14(9)：28.
[8]陈虹,白文成.自拟健脾通窍汤治疗AR50例[J].安徽中医临床杂志,2001,13(4)：274.
[9]刘绍炼.桂枝人参汤加味治疗AR60例[J].实用中医杂志,2007,23(2)：82.
[10]宋力伟.桂枝加附子汤治疗肾阳亏虚型AR48例[J].浙江中医杂志,2009,44(7)：
[11]何成莲,五苓散的临床运用[J].四川中医,2009,27(1)：123-124.
[12]高军,王静.梁拥军.中药治疗AR80例[J].山东医药,2004,44(13)：16.
[13]韩绘宇,王大海.补肾温肺胶囊治疗AR的远期疗效观察[J].中国中西医结合耳鼻咽喉科杂志,2002,10(5)：233-234.
[14]华刚,孟静.玉屏风散合苍耳子散加减治疗AR36例[J].河南中医,2005,25(3)：65.
[15]彭建中.清晨发病从肝论治验案举隅[J].北京中医药大学学报,2002,25(6)：66.
[16]常风云,常庚.防风善治AR[J].中医杂志,2003,44(7)：493.
[17]贾德蓉.浅谈“从肝入手、分期论治变应性鼻炎” [J].中医耳鼻喉科学研究杂志,2010,9(1)：32-33.
[18]郭强中,李云英.柴胡桂枝汤治疗AR经验[J].中医药导报,2008,7(4)：53-54.
[19]刘桂宇.加味玉屏风汤治疗ARI临床疗效观察[J].中国医疗前沿,2009,4(4)：32-33.
[20]邵晓颖.三合汤治疗变应性鼻炎132例[J].中医中药,2007,12(24)：77.
[21]李健民.中医辨证治疗变应性鼻炎36例[J].河南中医学院学报,2009,24(4)：88-89.
[22]邓少贤.黄芪桂枝五物汤加味治疗AR50例[J].实用中医内科杂志,2005,19(5)：
[1]方凤.小儿呼吸病临床诊疗指南[M].北京：人民军医出版社,2002.173-203.
[2]唐建华.过敏性疾病[M].上海：上海科学普及出版社,2002.10-34.155-160.
[3]Demoly P,BousquetJ.Drug allergy diagnosis work up.Allergy,2002,57(Suppl)72:37.
[4]ArroligaME,WagnerW,BobekMBetal.A Pilot study of Penicillin skin testing in Patients with a history of Penicillin allergy admitted to a medical ICU.Chest,2000,118:1106.
[5]Strom BL,Schinnar R,APterAJ etal.Absence of cross-reactivity between sulfonamide antibiotics and sulfonamide nonantibiotics.N Engl J Med,2003,349:1628.
[6]TurveySE,CroninB,Arnold AD etal.Antibiotic desensitization for the allergic Patient:5 Years of experience and practice. Ann Allergy Asthma Immunol.2004,92:426
[7]郭蕾.天人相应的理论思想文化基础,山西中医学院学报,2002,3(4)：4.
[8]肖洪彬,夏晓辉,朴赞斗等.消风散主要药效学及拆方研究[J].中国实验方剂学杂志,1999,5(4)：21-23.
[9].左祖英,唐恩洁,夏建平等.防风苍耳子水煎剂对小鼠免疫功能的影响叮[J].川北医学院学报,1997,12(3)：9-10.
[10]阮岩,冈本美孝,松崎全成.麻黄汤抗过敏作川的实验[J].中药新药与临床药理,2002,13(3)：152-154.
[11]陈子瑁.防风与刺蒺藜的药理实验研究[J].中成药,2003,25(9)：20-21.
[12]许筱颖.褪黑素及其合成限速酶在肾藏象调控理论中作用和地位的探讨与研究[D].北京中医药大学博士研究生学位论文,2007年
[13]刘晓燕,郭霞珍,刘燕池,等.“肾应冬”与性腺轴相关性的研究[J].中国医药学报,2003,18(9)：522-523.
[14]张明泉.下丘脑-垂体-甲状腺轴细胞分子机制的季节变化与中医肾本质研究的探讨[D].北京中医药大学博士研究生学位论文,2007年
[15]袁卫玲.肺应秋生理机制的研究——四季变化对大鼠肺脏免疫功能影响[D].北京中医药大学博士研究生学位论文,2008年
[1]Prieto J,Lensmar C,Roquet A,et al.Increased interleukin-13 mRNA expression in bronchoalveolar lavage cells of atopic patients with mild asthma after repeated low-dose allergen provocation.Respir Med,2000,94:806-814.
[2]毕爱华.医学免疫学.北京：人民军医出版社,1995：183-185.
[3]李立宇,刘学军,李国顺.过敏性哮喘患者血清IgE、IgG4、ECP、IL-2和IL-5水平变化及意义,山西医科大学学报,2006,37(8)：807.
[4]ChinratanapisitS,Tunsuriyawong P,Vichyanond P,et al.Chronicrhinosinusitis and recurrent nasal polyps in two children with IgGsubclass deficiency and review of the literature.J Med Assoc Thai,2005,88(Suppl8):S251-258.
[5]Molinero P,Soutto M,Benot S,et al.Melatonin is responsible for the nocturnal increase observ -ed in serum and thymus of thymosin alphal and thymulin concentrations.J Neuroimmunol,2000,103(2):180-188.
[6]周瑞祥,黄云梅.松果体及其褪黑素对大鼠胸腺细胞死亡信号通路的影响.解剖学报,2006,37(3)：303.
[7]马淑然,郭霞珍,刘燕池等.中医“肾应冬”生理机制与褪黑素关系的实验研究.北京中医药大学学报,2002,25(2)：19.
[8]刘志民.松果腺激素及其受体研究现状及展望.解放军医学杂志,2003,28(3)
[2]陈颖之,赵仓焕.刍议“肝旺于春”[J].贵阳中医学院学报,2009,31(5)：44-45.
[3]杨云霜.基于五脏应时发病基础的气温骤变诱发脑卒中机制的研究[J].北京中医药大学博士论文,2009.
[4]陈玉萍,马淑然,王庆国,赵燕.中医“肝应春”理论内涵的探讨[J].中华中医药杂志,2011,26(5)：1172-1175.
[5]袁卫玲,史楠楠,郭霞珍.过敏性疾患季节易感性与“肝应春”理论相关性探讨[J].中国中医药信息杂志,2011,18(4)：90-91.
[6]王玉芳.肝主升发与生发[J].中医药信息,2010.27(4)：6-7.
[7]肖昌湖,李鲁宁.季节指数预测法在精神病医院管理中的应用[J].赣南医学院学 报,2005,25(5)：610-611.
[8]郭玉秀.情感性精神障碍发病高峰时间与季节关系的圆形分布统计分析[J].中国民康医学,2004,11：662-663.
[9]郭永贵,郭玉秀,张衍军,等.情感性精神障碍患者发病高峰时间与季节关系的圆形分布统计分析[J].中国民康医学,2006,18(9)：745-746.
[10]张云,戴福强,唐怀瑞.情感性精神障碍首次发病与季节和气候的关系分析[J].临床中老年保健,2001,4(2)：85-86.
[11]代福强,陆万美,朱文波.231例情感性精神障碍的复发因素分析[J].四川精神卫生,2002,15(2)：89-91.
[12]金光亮,梁怡,郭霞珍等.有关抑郁症季节性发病机理的研究及其启示[J].北京中医药大学学报,1997,20(1)：15-16.
[13]徐砚通,郭霞珍.“时藏相应”生理机制实验研究[J].中国中医基础医学杂志,2001,7(5)：16-18.
[14]Sakamoto K, Kamo T, Nakadaira S, et al. An ation wide survey of seasonal affective disorder at 53 outpatientuniversity clinics in Japan[J].Acta Psychiatr Scand,1993,87: 258-265.
[15]David S.Schlager.Early-Morning Administration of Short-Acting β Blockers for Treatment of Winter Depress[J].Am J Psychiatry,1994,151 (9):1383-1385.
[16]顾中范.褪黑激素与季节性情感性精神病[J].上海精神医学,1990,2(4)：206.
[17]In-Young Y, Denirl FK.. Luteinizing hormonr following light exposure in healthy young men [J]. Neuroscience Letters,2003,341:25-28.
[18]吴岳峰,高成阁.引起精神分裂症患者出生的季节性差异因素[J].国外医学医学地理杂志,2006,27(3)：137-141.
[19]工海成,刘庆武,张敏.分裂样精神病发病与季节节气关系的序列分析[J].郴州医学高等专科学校学报,2003,5(3)：10-12.
[20]吴燕燕,乔振刚.“肝木主病”说指导春季临床治疗[J].光明中医杂志,1995,(6)：5-6.
[21]赵满华.时间医学验案选例[J].辽宁中医杂志,1983,7(4)：32-34.
[22]任兰民,李志华.精神分裂症的发病季节性调查.预防医学文献信息,2000,6(4)：345.
[23]计庆明、顾世芬等.精神疾病与气象要素相关性研究.山东精神医学,2003,16(2)：65—67.
[24]陈红.3037例精神分裂症出生与入院季节的关系分析.中国民康医学杂志,2004,16(5)：271-272.
[1]林颖,陈达灿.变态反应性疾病发病相关因素的研究进展[J].中国中西医结合皮肤性病学杂志,2005,4(1)：60-63
[2]谈欧,王德钧.韩树人教授淡“哮喘专主于风”[J].南京中医药大学学报,2007,23(4)：259.
[3]宋苹“过敏煎”联合开瑞坦治疗过敏性咳嗽30例[J].实用医学杂志.2009,25(1)：164.
[4]李庆生.过敏性疾病应重视病证结合从“风”论治用药[J].云南中医中药杂 志,1998,19(3)：9.[5]栗庆林.从肝从风论治支气管哮喘[J].光明中医,2004,19(6)：18.
[6]翟文生,朱珊.过敏性咳嗽从肝论治的临床研究[J].中国医药学报,2003,18(2)：123.
[7]范瑞强,廖元兴.中西医结合临床皮肤性病学[M].广州：世界图书出版公司,2003,462.
[8]李元文,张丰川,周德瑛.辨证治疗特应性皮炎35例[J].北京中医药大学学报,2002,25(5)：69-70.
[9]余土根.中西医结合诊治特应性皮炎的思路[J].浙江中西医结合杂志,2005,15(8)：463-464.
[10]李彦军,刘澈.马淑然教授运用加味过敏煎治疗过敏性疾病验案举隅.中国中医药信息杂志.2010,7(7)：89-90.
[11]马群力.消风散治疗过敏性疾病验案5则[J].现代中西医结合杂志,2004,13(21)：2874-2875.
[12]程丑夫.过敏性疾病当从风论治[J].江苏中医药,2002,23(10)：1-3.
[13]毕莲.傅汝林治疗过敏性紫瘫经验[J].辽宁中医杂志,2009,36(3)：345-346.
[14]尹璐.尹铁汉治疗过敏性疚病的经验叮[J]医学创新研究,2006,]2(3)：12.
[15]张谈.张迪蛟治疗过敏性疾病验案四则[J].江西中医药,2005,36(271)：9-10.
[16]陈斌.自拟消风饮治疗过敏性疾病30例[J]Chinese Journal of the Practieal Chinese with Modem Medieine,2005,VOL.(18)NO.23.
[17]林蔚达.试述过敏性鼻炎的中医辨治[J].浙江中医杂志,2009,6(44)：455.
[18]Vandenbulcke L, Bachert C, Van Cauwenberge P, et al. The innate immune system and its role in allergic disorders[J]. Int Arch Allergy Immunol,2006,139 (2):159-165.
[19]Renz H,Smith H R, Henson J E,et al. Aerosolized antigen exposure without adjuvant causes increased IgE production and increased airway responsiveness in the mouse[J]. J Allergy Clin Immunol,1992,89(6):1127-1138.
[20]陈木开,廖绮曼,林志斌.遗传过敏性皮炎血清特异性IgE分析[J].中国皮肤性病学杂志,2000,14：151-152.
[21]庄丽华.吸入性变应原诱导的皮肤迟发相反应[J].国外医学,皮肤性病学分册,1995,21：328-331.
[22]刘彦群,赵辨.季节性面部接触性皮炎致病因素及其发病机理的初步探讨[J].中华皮肤科杂志,1992,25(4)：224.
[23]庄丽华,赵辨.43例季节性接触性皮炎皮肤实验研究[J].中华皮肤科杂志,1996,29(1)：18-21.
[24]严道金,赵辨.季节性接触性皮炎花粉斑贴实验阳性部位皮肤IgE检测[J].临床皮肤科杂志,1998,31(4)：250.
[25]赵辨.临床皮肤病学[M].3版.南京：江苏科学技术出版社,2001：63.
[26]程少为,刘春玲,兰东,等.荨麻疹患者特异性IgER及过敏原检测与分析[J].中华皮肤科杂志,2003,36(11)：637-639.
[27]张金桃,陈瑾萍,罗佩谊等.特应性皮炎和慢性荨麻疹儿童IgE检测及变应原分析[J].广东医学杂志,2007,28(1())：1688-1690.
[28]李林峰.接触性皮炎与皮肤变态反应[M].2版.北京:北京大学医学出版社,2003：201.
[29]李明华,殷凯生,朱栓立.哮喘病学[M].北京：人民卫生出版社,1998：47.
[30]李明华,殷凯生,朱栓立.哮喘病学[M].北京：人民卫生出版社,1998：113.
[31]MacGlashan DW, Bochner BS, Adelmen DC, et al.Down-regulation of Fc ε R Ⅰ expression on human basophils duringin vivotreatment of atopic patients with anti-IgE antibody[J].J Immunol,1997,158(3):1438.
[32]MacGlashan DW, Mckenzie-White J, Chichester K, et al.In vitro regulation of Fc ε R Ⅰ expression on human basophils by IgE antibody [J]. Blood,1998,91(5):1633.
[33]Bang LM, Plosker GL. Spotlight on omalizumab in aller-gic asthma[J]. Biol Drugs, 2004,18(6):415.
[34]Hvatum M, Scott H, Brandtzaeg P. Serum IgG subclass antibodies to a variety of food antigens in patients with coeliac disease [J]. Gut,1992,33(5):628632.
[35]王福新,陈丹绿,周世良等.变态反应疾病血清IgE和IgG分析[J].江西医学院学报,2006,46(1)：183.
[36]张利,赖蓉,尹洁.湿疹患者食物过敏原特异性IgG检测及意义[J].四川医学,2009,30(2)：256-257.
[37]De Waal Malefyt R, Figdor CG, Huijkens R,et al. Effects of IL-13 on phenotype, cytokine production and cytotoxic function of human monocyte. Comparison with IL-4 and modulation by IFN-γ or IL-10[J]. J Immunol,2000,158:6370-6381.
[38]Briere F, Bridon JM, Servet C,et al.IL-10 and IL-13 as B cell growth and differentiation factors[J]. Nouv Rev Fr Hematol,2000,40(3):233-235.
[39]Kornmann M, Kleeff J, Debinski W,et al. Pancreatic cancer cells express interkeukin-13 and-4 receptors, and their growth is inhibited by pseudomonas exotoxin coupled to interleukin-13 and-4[J]. Anticancer Res,1999,19:125-131.
[40]De Waal Malefyt R, Figdor CG, Richard H, et al. Effecte of IL-13 on phenotype, cytokine production, and cytotosin function of human monocytes:comparison with IL-4 and modulation by IFR-r or IL-10. J Immunol,1993;151(11):6370.
[41]Tengvall-Linder M,et al.Clin-Exp-Allergy,1996;26(11):1286-97.
[42]Ying S,et al.J immunol,1997;158(10):5050-7.
[43]Burrows B, Sears MR, Flannery EM,et al. Relation of the course of bronchial responsiveness from age 9 to age 15 to allergy [J]. Am J Respir Crit Care Med,1995,152(4 Pt 1):1302-1308.
[44]Saha SK, Berry MA, Parker D, et al. Increased sputum and bron-chial biopsy IL - 13 expression in severe asthma[J]. J Allergy ClinImmunol.2008,121 (3):685-691.
[45]Zhang Y, et al.J-Exp-Med,1996; 184(4):1507-12.
[46]Kotsimbos TC, et al.Proe-Asso-Am-Physicians,1996;108(5):368-73.
[47]Nasser J. et al.Am-J-Respir-Crit-Care-Med,1997; 155(3):845-51.
[48]Zhu Z, Zheng T, Homer RJ, et al.Acidic mammalian chitinase in asthmatic Th2 inflammation and IL - 13 pathway activation[J]. Sci-ence,2004,304(5677):1678 1682.
[49]Corren J. Lemanske RF Jr, et al.Lebrikizumab treatment in adults with asthma[J]. N Engl J Med,2011,[Epub ahead of print].
[50]Diaz-Sanchez D.et al.J-Allergy-Clin-immunol,1996;98(1):114-23.
[51]Pawankar RU,et al.Am-J-Respir-Crit-Care-Med,1995;152(6pt1):2059-67.
[52]Ghaffar O,et al.Am-J-Respir-Crit-Care-Med,1995;152(6pt1):2059-67.
[1]Chen Q, Wei W. Effects and mechanisms of melatonin on inflammatory and immune responses of adjuvant arthritis rat[J].Int Immunopharmacol,2002,2(10):1443-1449.
[2]Provinciali M, Di Stefano G, Bulian D, Tibaldi A,Fabrs N. Effect of melatonin and pineal grafting on thymocyte apoptosis in aging mice[J].Mech Aging Dev,1996,90(1):1-19.
[3]Dubocovich ML, Markowska M. FunctionalMT1 and MT2 melaton in receptors in mammals[J].Endocrine,2005,27(2):101-110.
[4]Oner H, Kus I, Oner J, et al. Possibleeffects of melatonin on thymus gland after pinealectomy in rats[J]. Neuro EndocrinolLett,2004,25(1-2):115-118
[5]Singh SS, Haldar C, RaiS. Melatonin and differential effectofL-thyroxine on immune system of Indian tropicalbird Perdicula asiatica[J]. Gen Comp Endocrino,1 2006,145(3): 215-221
[6]Champney TH, Prado J, Youngblood T, et, al. Immune responsiveness of splenocytes after chronic daily melatonin administration in male Syrian hamsters[J].Immunol Lett,1997, 58(2):95-100.
[7]徐峰,李经才,杨迎暴,等.褪黑素对光照制度改变影响的对抗作用[J].中国药理学通报,1994,(10)：448.
[8]秦承德.生理科学研究进展[M].沈阳：辽沈书社,1993,123-124.
[9]Arias J, Melean E,Valero N, et al. Effect of melatonin on lymphocyte proliferation and production of interleukin-2(IL-2) and interleukin-1 beta(IL-1beta) in mice splenocytes[J]. Invest Clin,2003,44(1):40-50.
[10]Maestroni GJ, Conti A. Melatonin and the immune-hematopoietic system therapeutic and adverse pharmacological correlates! JJ.Neuroimmunomodula tion,1996,3(6) 325.
[11]成令忠,钟翠平,蔡文琴.现代组织学[M].上海：上海科学技术文献出版社,2003,580.
[12]刘爱国,杨默,李志光,等.褪黑素对照射后小鼠血小板造血的保护作用[J].中华放射医学与防护杂志,2005,25(6)：538.
[13]Lissoni P, Mandala M, Rossini F, et al. (irowth factors:thrombopoietic property of the pineal hormone melatonin[J]. Hematology,1999,4(4):335-343.
[14]Rapozzi V, Zorzet S, Comelli M, Mavelli 1, Perissin L, Giraldi T. Melatonin decreases bone marrow and lymphatic toxicity of adriamycin in mice bearing TLX5 lymphoma[J]. Life Sci,1998,63(19):1701-1711.
[15]Drazen DL, Jasnow AM, Nelson RJ, Demas GE. Exposure to short days, but not short term melatonin, enhances humoral immunity of male Syrian hamsters (Mesocricetus auratus)[J],Pineal Res,2002,33(2):118-120.
[16]Shaji AV, Kulkarni SK, Agrewala JN. Regulation of secretion of IL-4 and IgG lisotype by melatonin stimulated ovalbumin specific T cells[J]. Clin Exp Immunol,1998,111(1):181-185.
[17]徐峰,李经才,杨迎暴,等.褪黑素对光照制度改变影响的对抗作用[J].中国药理学通报,1994,(10)：448.
[18]秦承德.生理科学研究进展[M].沈阳：辽沈书社,1993,123-124.
[19]Arias J, Melean E,Valero N, et al. Effect of melatonin on lymphocyte proliferation and production of interleukin-2(IL-2) and interleukin-1 beta(IL-lbeta) in mice splenocytes[J]. Invest Clin,2003,44(1):40-50.
[20]Drazen DL, Jasnow AM, Nelson RJ, Demas GE. Exposure to short days, but not short term melatonin, enhances humoral immunity of male Syrian hamsters (Mesocricetus auratus)[J],Pineal Res,2002,33(2):118-120.
[21]Shaji AV, Kulkarni SK, Agrewala JN. Regulation of secretion of IL-4 and IgG lisotype by melatonin stimulated ovalbumin specific T cells[J]. Clin Exp Immunol,1998,111(1):181-185.
[22]VigoreL, Messina G, Brivio F, et al.Psychoneuroendocrine modulation of regulatory T lymphocyte system:in vivo and in vitro effects of the pineal immunomodulating hormone melatonin[J]. In Vivo,2010,24(5):787-789.
[23]何维.医学免疫学[M].人民卫生出版社,2005,157.
[24]Wichmann MW, Zellweger R, DeMaso, Ayala A, et, al. Melatonin admini strati on attenuates depressed immune functions trauma hemorrhage[J].Surg Res,1996,63(1):256-262.
[25]LissoniP, Rovelli F, Brivio F.Circadian secretion of IL-2, IL-12,IL-6 and IL-10 in relation to the light/dark rhythm of the pineal hormone melatonin in healthy humans[J]. Nat Immun,1998,16(1):1-5.
[26]Sharman KG, Sharman EH, Yang E, et al. Dietary melatonin selectively reverses age related changes in cortical cytokine mRNA levels, and their responses to an inflamematory stimulus [J]. Neurobiol Aging,2002,23 (4):633-638.
[27]迟素敏.内分泌生理学[M].西安：第四军医大学出版社,2006,143-144.
[28]Moore CB, SiopesTD. Melatonin enhances cellular and humoral im-mune responses in the Japanese quail(Coturnix coturnix japonica)via an opiatergic mechanism[J].Gen Comp Endocrinol,2003,131(3):258-263.
[29]黄颖苏.松果腺褪黑素对下丘脑-垂体-肾上腺轴的调节作用[J].国外医学分册：内分泌代谢杂志,2001,21(2)：88.
[30]魏伟.褪黑素对炎症免疫和痛反应的作用及其机制[J].安徽医科大学学报.1999,(34)2：159-160
[31]Bonilla E, Valero N, Chacin-Bonilla L,et al. Melatonin and viral infections[J].JPinealRes, 2004,36(2):73-79.
[32]Carrillo-VicoA, LardonePJ, NajiL,et al. Beneficial pleiotropic actions of melatonin in an experimental model of septic shock in miceregulation of pro-/anti-inflammatory cytokine network, protection against oxidative damage and anti-apoptotic effects[J].JPinealRes,2005, 39(4):400-408.
[33]ImamogluM, Cay A, Cobanoglu U,et al. Effects of melatonin on suppression of renal scarring in experimental model of pyelonephritis[J].Urology,2006,67(6):1315-1319.
[34]Maestroni GJ.The immunotherapeutic potential of melationin[J]. Expert Opin Investig Drugs,2001,10:467
[35]imenez-CalianiAJ, Jimenez-Jorge S, Molinero P,et al. Dual effect of melatonin as proinflammatory and antioxidant in collagen-induced arthritis in rats[J].JPinealRes,2005, 38(2):93-99.
[36]Haase CG, TinnefeldM, Faustmann PM. The influence of immuno-modulation on psycho-neuroimmuno logical functions in benign multiple sclerosis[J].Neuroimmuno modulation,2004,11(6):365-372.
[37]HaaseCG, Faustmann PM. Benignmultiple sclerosis is characterized by a stable neuroimmuno logic network[J].Neuroimmuno modulation,2004,11(4):273-277.
[38]Jimenez-Caliani AJ, Jimenez-Jorge S, Molinero P,et al. Sex-de-pendent effect of melatonin on systemic erythematosus lupus developed inMrl/Mpj-Faslprmice:it ameliorates the disease course in females, whereas it exacerbates it in males[J].Endocrinology, 2006,147(4):1717-1724.
[39]Maestroni GJ.The photoperiod transducer melatonin and the immune hematopoietic system[J].J Photochem Photobiol B,1998,43:186.
[40]Teplitzky SR,kiefer TL,Cheng Q,et al.Chemoprevention of NMU-induced rat mammary carcinoma with the combination of melatonion of melatonion and 9-cis-retinoic acid[J].Cancer Lett,2001.168:155.
[41]何绍雄.时间药理学与时间治疗学[M].天津：天津科学技术出版社,1994,40.
[1]刘兆琳,侯慎英.赵立新.过敏煎治疗变应性鼻炎90例[J].河北中医2005,27(3)：227.
[2]朱正民,耿以安.陈虹.小青龙汤加减治疗AR[J].湖北中医杂志,2004,26(5)：38-39.
[3]张兴学.玉屏风散加减治疗AR63例[J].现代中西医结合杂志,2005,14(4)：493.
[4]吴朝晖.蔡氏抗敏鼻炎方治疗AR 35例[J].安徽中医临床杂志,2003,15(6)：469.
[5]葛荫茹,郑薇.鼻鼽中医治疗79例体会[J].当代医学,2007,(9)：92-93.
[6]苏清学.补中益气汤新用[J].中国民间疗法,.2007,1 5(1)：33-34.
[7]魏萍,魏棠,肖风英.益气通窍汤治疗AR30例[J].中国民间疗法,2006,14(9)：28.
[8]陈虹,白文成.自拟健脾通窍汤治疗AR50例[J].安徽中医临床杂志,2001,13(4)：274.
[9]刘绍炼.桂枝人参汤加味治疗AR60例[J].实用中医杂志,2007,23(2)：82.
[10]宋力伟.桂枝加附子汤治疗肾阳亏虚型AR48例[J].浙江中医杂志,2009,44(7)：
[11]何成莲,五苓散的临床运用[J].四川中医,2009,27(1)：123-124.
[12]高军,王静.梁拥军.中药治疗AR80例[J].山东医药,2004,44(13)：16.
[13]韩绘宇,王大海.补肾温肺胶囊治疗AR的远期疗效观察[J].中国中西医结合耳鼻咽喉科杂志,2002,10(5)：233-234.
[14]华刚,孟静.玉屏风散合苍耳子散加减治疗AR36例[J].河南中医,2005,25(3)：65.
[15]彭建中.清晨发病从肝论治验案举隅[J].北京中医药大学学报,2002,25(6)：66.
[16]常风云,常庚.防风善治AR[J].中医杂志,2003,44(7)：493.
[17]贾德蓉.浅谈“从肝入手、分期论治变应性鼻炎” [J].中医耳鼻喉科学研究杂志,2010,9(1)：32-33.
[18]郭强中,李云英.柴胡桂枝汤治疗AR经验[J].中医药导报,2008,7(4)：53-54.
[19]刘桂宇.加味玉屏风汤治疗ARI临床疗效观察[J].中国医疗前沿,2009,4(4)：32-33.
[20]邵晓颖.三合汤治疗变应性鼻炎132例[J].中医中药,2007,12(24)：77.
[21]李健民.中医辨证治疗变应性鼻炎36例[J].河南中医学院学报,2009,24(4)：88-89.
[22]邓少贤.黄芪桂枝五物汤加味治疗AR50例[J].实用中医内科杂志,2005,19(5)：
[1]方凤.小儿呼吸病临床诊疗指南[M].北京：人民军医出版社,2002.173-203.
[2]唐建华.过敏性疾病[M].上海：上海科学普及出版社,2002.10-34.155-160.
[3]Demoly P,BousquetJ.Drug allergy diagnosis work up.Allergy,2002,57(Suppl)72:37.
[4]ArroligaME,WagnerW,BobekMBetal.A Pilot study of Penicillin skin testing in Patients with a history of Penicillin allergy admitted to a medical ICU.Chest,2000,118:1106.
[5]Strom BL,Schinnar R,APterAJ etal.Absence of cross-reactivity between sulfonamide antibiotics and sulfonamide nonantibiotics.N Engl J Med,2003,349:1628.
[6]TurveySE,CroninB,Arnold AD etal.Antibiotic desensitization for the allergic Patient:5 Years of experience and practice. Ann Allergy Asthma Immunol.2004,92:426
[7]郭蕾.天人相应的理论思想文化基础,山西中医学院学报,2002,3(4)：4.
[8]肖洪彬,夏晓辉,朴赞斗等.消风散主要药效学及拆方研究[J].中国实验方剂学杂志,1999,5(4)：21-23.
[9].左祖英,唐恩洁,夏建平等.防风苍耳子水煎剂对小鼠免疫功能的影响叮[J].川北医学院学报,1997,12(3)：9-10.
[10]阮岩,冈本美孝,松崎全成.麻黄汤抗过敏作川的实验[J].中药新药与临床药理,2002,13(3)：152-154.
[11]陈子瑁.防风与刺蒺藜的药理实验研究[J].中成药,2003,25(9)：20-21.
[12]许筱颖.褪黑素及其合成限速酶在肾藏象调控理论中作用和地位的探讨与研究[D].北京中医药大学博士研究生学位论文,2007年
[13]刘晓燕,郭霞珍,刘燕池,等.“肾应冬”与性腺轴相关性的研究[J].中国医药学报,2003,18(9)：522-523.
[14]张明泉.下丘脑-垂体-甲状腺轴细胞分子机制的季节变化与中医肾本质研究的探讨[D].北京中医药大学博士研究生学位论文,2007年
[15]袁卫玲.肺应秋生理机制的研究——四季变化对大鼠肺脏免疫功能影响[D].北京中医药大学博士研究生学位论文,2008年
[1]Prieto J,Lensmar C,Roquet A,et al.Increased interleukin-13 mRNA expression in bronchoalveolar lavage cells of atopic patients with mild asthma after repeated low-dose allergen provocation.Respir Med,2000,94:806-814.
[2]毕爱华.医学免疫学.北京：人民军医出版社,1995：183-185.
[3]李立宇,刘学军,李国顺.过敏性哮喘患者血清IgE、IgG4、ECP、IL-2和IL-5水平变化及意义,山西医科大学学报,2006,37(8)：807.
[4]ChinratanapisitS,Tunsuriyawong P,Vichyanond P,et al.Chronicrhinosinusitis and recurrent nasal polyps in two children with IgGsubclass deficiency and review of the literature.J Med Assoc Thai,2005,88(Suppl8):S251-258.
[5]Molinero P,Soutto M,Benot S,et al.Melatonin is responsible for the nocturnal increase observ -ed in serum and thymus of thymosin alphal and thymulin concentrations.J Neuroimmunol,2000,103(2):180-188.
[6]周瑞祥,黄云梅.松果体及其褪黑素对大鼠胸腺细胞死亡信号通路的影响.解剖学报,2006,37(3)：303.
[7]马淑然,郭霞珍,刘燕池等.中医“肾应冬”生理机制与褪黑素关系的实验研究.北京中医药大学学报,2002,25(2)：19.
[8]刘志民.松果腺激素及其受体研究现状及展望.解放军医学杂志,2003,28(3)