固始鸡—安卡鸡F_2代资源群PPARα基因多态性的研究
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摘要
PPARα基因属于核激素受体超家族的成员之一,在脂类代谢中起着重要作用。本研究采用PCR-SSCP、CRS-PCR-RFLP、DNA测序等方法检测了固始鸡-安卡鸡F_2代资源群849只个体PPARα不同基因片段的多态性,对单核苷酸多态性进行了群体遗传学分析,同时进行了基因多态位点与经济性状的关联分析,结果如下:
1、经过PCR-SSCP检测发现Pl扩增片段上有一个A→T错义突变(g.A23884T),通过BglⅡ酶切出现AA和AB两种基因型;P3扩增片段上发现一个G→A错义突变(g.G29201A),通过BsiEI酶切出现CC和CD两种基因型;P4扩增片段上发现一个T→C同义突变(g.T32311C),通过BspHI酶切出现EE、EF、FF三种基因型。
2、将鸡PPARα基因不同位点多态性与屠体性状进行相关分析,结果表明,P1位点不同基因型对肝脏重和肝脏率的影响差异显著(P<0.05);P3位点不同基因型对胰腺率的影响差异显著(P<0.05);P4位点FF基因型的肌胃重大于EF基因型,差异极显著(P<0.01);EF和FF基因型的肝脏率与EE基因型相比,差异均显著(P<0.05)。
3、将鸡PPARα基因不同位点多态性与脂肪、肉质性状进行相关分析,结果表明,P1位点不同基因型对腹脂重、腹脂率以及胸肌失水率的影响差异显著(P<0.05);P4位点不同基因型对腹脂重、腹脂率的影响差异极显著(P<0.01),EE型和EF型的腹脂重、腹脂率大于FF型,差异均显著(P<0.05)。
4、将鸡PPARα基因不同位点多态性与生长性状进行相关分析,结果表明,P3位点不同基因型对出生重和2周龄体重的影响差异极显著(P<0.01),CC基因型的8、12周龄胫长、8周龄骨盆宽大于CD基因型,差异显著(P<0.05);CD基因型的4周龄胸角大于CC基因型,差异显著(P<0.05);P4位点不同基因型对6周龄体重、4周龄体斜长的影响差异极显著(P<0.01),对4周龄体重、4周龄胫围、4、8、12周龄胸骨长、4周龄骨盆宽影响差异显著(P<0.05)。
5、将鸡PPARα基因不同位点多态性与血液生理生化指标进行相关分析,结果表明,P1位点不同基因型对总蛋白、肌酐的影响差异显著(P<0.05);P4位点EE、EF基因型的高密度脂蛋白与FF型相比差异显著(P<0.05)。
6、将鸡PPARα基因三个多态位点的组合基因型与经济性状进行关联分析,结果表明,不同组合对屠体性状中肌胃重、肝脏率、胰腺率的影响差异显著;对生长性状中出生重、6周龄体重的影响差异极显著(P<0.01),对4周龄体重、8、12周龄胫围、12周龄胸骨长的影响差异显著(P<0.05);对脂肪和肉质性状中腹脂重、腹脂重率以及胸肌失水率的影响差异显著,基因型组合ABCCEE的白蛋白含量与AACDEF相比,差异极显著(P<0.01)。
PPARαis a member of the nuclear receptor super family which has been proposed to play key roles in lipid and carbohydrate metabolism.This study applies PCR-SSCP, CRS-PCR-RFLP, DNA sequencing and PCR-RFLP techniques to detect the polymorphism of PPAR gene from 849 chickens of Gushi-Anak F_2 Resource Population.
The results were as follows:
1. Applying PCR-SSCP method to scan the SNPs, an A/T non-synonymous mutation in the P1 amplified fragment(g.A23884T)was detected by restriction enzyme BglII, resulting in two genotypes that named AA genotype and AB genotype; a G/A non-synonymous mutation in the P3 amplified fragment(g.G29201A)was detected by restriction enzyme BsiEI, resulting in two genotypes that named CC genotype and CD genotype; a T/C silent mutation in the P4 amplified fragment(g.T32311C)was detected by restriction enzyme BspHI, resulting in three genotypes that named EE genotype, EF genotype and FF genotype.
2. Estimating the correlation analysis between the polymorphisms and carcass trait,the result showed that different genotypes of P1 locus on the liver weight and liver weight rate were significantly different (P<0.05);different genotypes of P3 locus on the pancreas weight rate was significantly different (P<0.05); different genotypes of P4 locus had effects on the gizzard weight and liver weight rate ,the gizzard weight of FF genotype was higher than EF genotype (P<0.01),comparing with EE genotype, the liver weight rate between EF and FF genotypes were significantly different (P<0.05).
3. Estimating the correlation analysis between the polymorphisms and fat, meat quality traits, the result showed that different genotypes of P1 locus on the abdominal fat weight, abdominal fat rate and water loss rate of breast muscle were significantly different (P<0.05); different genotypes of P4 locus had effects on the abdominal fat weight and abdominal fat rate, the differences were very significant (P<0.01), the abdominal fat weight and abdominal fat rate of EE and EF genotypes were higher than FF genotype (P<0.05).
4. Estimating the correlation analysis between the polymorphisms and growth trait, the result showed that the different genotypes of P3 locus influenced the birth weight and body weight at the age of 2 weeks very significantly (P<0.01),the shank girth at the age of 8 and 12weeks, pelvis breadth at the age of 8 weeks of CC genotype greater than CD genotype, the difference was significant (P<0.05),the breast angle of CD genotype greater than CC genotype at the age of 4 weeks (P<0.05), EE,EF and FF genotypes of P4 locus had different effects on body weight at the age of 6 weeks,body slanting length at the age of 4 weeks (P<0.01) and body weight,shank girth,pelvis breadth at the age of 4 weeks,length of breastbone at the age of 4,8 and12 weeks (P<0.05).
5. Estimating the correlation analysis between the polymorphisms and physiological and biochemical index, the result showed that different genotypes of P1 locus on the total protein and creatinine were significantly different (P<0.05); compared with FF genotype, EE, EF genotypes of P4 site had effects on high density lipoprotein (P<0.05).
6. Estimating the correlation analysis between com-genotypes of PPARαgene and economical traits,the result showed that different com-genotypes had significant effects on the liver weight, the pancreas weight rate of carcass trait; different com-genotypes had extremely significant effects on the birth weight and body weight at the age of 6 weeks(P<0.01)and significant effects on body weight at the age of 4weeks, the shank girth at the age of 8 and 12weeks, length of breastbone at the age of 12 weeks(P<0.05) of growth trait;different com-genotypes had significant effects on the abdominal fat weight, abdominal fat rate and water loss rate of breast muscle of fat and meat quality traits; the difference of albumin content between ABCCEE and AACDEF was very significant(P<0.01).
1、经过PCR-SSCP检测发现Pl扩增片段上有一个A→T错义突变(g.A23884T),通过BglⅡ酶切出现AA和AB两种基因型;P3扩增片段上发现一个G→A错义突变(g.G29201A),通过BsiEI酶切出现CC和CD两种基因型;P4扩增片段上发现一个T→C同义突变(g.T32311C),通过BspHI酶切出现EE、EF、FF三种基因型。
2、将鸡PPARα基因不同位点多态性与屠体性状进行相关分析,结果表明,P1位点不同基因型对肝脏重和肝脏率的影响差异显著(P<0.05);P3位点不同基因型对胰腺率的影响差异显著(P<0.05);P4位点FF基因型的肌胃重大于EF基因型,差异极显著(P<0.01);EF和FF基因型的肝脏率与EE基因型相比,差异均显著(P<0.05)。
3、将鸡PPARα基因不同位点多态性与脂肪、肉质性状进行相关分析,结果表明,P1位点不同基因型对腹脂重、腹脂率以及胸肌失水率的影响差异显著(P<0.05);P4位点不同基因型对腹脂重、腹脂率的影响差异极显著(P<0.01),EE型和EF型的腹脂重、腹脂率大于FF型,差异均显著(P<0.05)。
4、将鸡PPARα基因不同位点多态性与生长性状进行相关分析,结果表明,P3位点不同基因型对出生重和2周龄体重的影响差异极显著(P<0.01),CC基因型的8、12周龄胫长、8周龄骨盆宽大于CD基因型,差异显著(P<0.05);CD基因型的4周龄胸角大于CC基因型,差异显著(P<0.05);P4位点不同基因型对6周龄体重、4周龄体斜长的影响差异极显著(P<0.01),对4周龄体重、4周龄胫围、4、8、12周龄胸骨长、4周龄骨盆宽影响差异显著(P<0.05)。
5、将鸡PPARα基因不同位点多态性与血液生理生化指标进行相关分析,结果表明,P1位点不同基因型对总蛋白、肌酐的影响差异显著(P<0.05);P4位点EE、EF基因型的高密度脂蛋白与FF型相比差异显著(P<0.05)。
6、将鸡PPARα基因三个多态位点的组合基因型与经济性状进行关联分析,结果表明,不同组合对屠体性状中肌胃重、肝脏率、胰腺率的影响差异显著;对生长性状中出生重、6周龄体重的影响差异极显著(P<0.01),对4周龄体重、8、12周龄胫围、12周龄胸骨长的影响差异显著(P<0.05);对脂肪和肉质性状中腹脂重、腹脂重率以及胸肌失水率的影响差异显著,基因型组合ABCCEE的白蛋白含量与AACDEF相比,差异极显著(P<0.01)。
PPARαis a member of the nuclear receptor super family which has been proposed to play key roles in lipid and carbohydrate metabolism.This study applies PCR-SSCP, CRS-PCR-RFLP, DNA sequencing and PCR-RFLP techniques to detect the polymorphism of PPAR gene from 849 chickens of Gushi-Anak F_2 Resource Population.
The results were as follows:
1. Applying PCR-SSCP method to scan the SNPs, an A/T non-synonymous mutation in the P1 amplified fragment(g.A23884T)was detected by restriction enzyme BglII, resulting in two genotypes that named AA genotype and AB genotype; a G/A non-synonymous mutation in the P3 amplified fragment(g.G29201A)was detected by restriction enzyme BsiEI, resulting in two genotypes that named CC genotype and CD genotype; a T/C silent mutation in the P4 amplified fragment(g.T32311C)was detected by restriction enzyme BspHI, resulting in three genotypes that named EE genotype, EF genotype and FF genotype.
2. Estimating the correlation analysis between the polymorphisms and carcass trait,the result showed that different genotypes of P1 locus on the liver weight and liver weight rate were significantly different (P<0.05);different genotypes of P3 locus on the pancreas weight rate was significantly different (P<0.05); different genotypes of P4 locus had effects on the gizzard weight and liver weight rate ,the gizzard weight of FF genotype was higher than EF genotype (P<0.01),comparing with EE genotype, the liver weight rate between EF and FF genotypes were significantly different (P<0.05).
3. Estimating the correlation analysis between the polymorphisms and fat, meat quality traits, the result showed that different genotypes of P1 locus on the abdominal fat weight, abdominal fat rate and water loss rate of breast muscle were significantly different (P<0.05); different genotypes of P4 locus had effects on the abdominal fat weight and abdominal fat rate, the differences were very significant (P<0.01), the abdominal fat weight and abdominal fat rate of EE and EF genotypes were higher than FF genotype (P<0.05).
4. Estimating the correlation analysis between the polymorphisms and growth trait, the result showed that the different genotypes of P3 locus influenced the birth weight and body weight at the age of 2 weeks very significantly (P<0.01),the shank girth at the age of 8 and 12weeks, pelvis breadth at the age of 8 weeks of CC genotype greater than CD genotype, the difference was significant (P<0.05),the breast angle of CD genotype greater than CC genotype at the age of 4 weeks (P<0.05), EE,EF and FF genotypes of P4 locus had different effects on body weight at the age of 6 weeks,body slanting length at the age of 4 weeks (P<0.01) and body weight,shank girth,pelvis breadth at the age of 4 weeks,length of breastbone at the age of 4,8 and12 weeks (P<0.05).
5. Estimating the correlation analysis between the polymorphisms and physiological and biochemical index, the result showed that different genotypes of P1 locus on the total protein and creatinine were significantly different (P<0.05); compared with FF genotype, EE, EF genotypes of P4 site had effects on high density lipoprotein (P<0.05).
6. Estimating the correlation analysis between com-genotypes of PPARαgene and economical traits,the result showed that different com-genotypes had significant effects on the liver weight, the pancreas weight rate of carcass trait; different com-genotypes had extremely significant effects on the birth weight and body weight at the age of 6 weeks(P<0.01)and significant effects on body weight at the age of 4weeks, the shank girth at the age of 8 and 12weeks, length of breastbone at the age of 12 weeks(P<0.05) of growth trait;different com-genotypes had significant effects on the abdominal fat weight, abdominal fat rate and water loss rate of breast muscle of fat and meat quality traits; the difference of albumin content between ABCCEE and AACDEF was very significant(P<0.01).
引文
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