氧化魔芋葡甘聚糖—大豆分离蛋白基空心胶囊囊材研究
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摘要
本文以天然植物多糖魔芋葡甘聚糖、大豆分离蛋白为原料,通过化学改性合成一种成膜性好的材料,并将其应用于空心胶囊囊材。主要研究内容如下:
1、对魔芋葡甘聚糖进行氧化改性,并测定溶胶的粘度和成膜后膜的力学性能、阻湿性能和阻气性能等指标。结果表明,氧化最佳反应条件为:2.4%H2O2(v/v),50℃反应5h,膜的拉伸强度为15.38MPa,断裂伸长率为18.7%,吸湿增重为23.41%,透湿系数为9.0×10-9g·cm/cm2·s·Pa,膜的综合性能较KGM有较大提高。氧化后分子链断裂,分子量降低。通过激光光散射检测其重均分子量为2.889×105 g/mol,较KGM下降了70.5%。
2、对氧化魔芋葡甘聚糖与大豆分离蛋白进行交联改性,制备出一种新的材料氧化魔芋葡甘聚糖-大豆分离蛋白,确定最佳反应条件为:氧化魔芋葡甘聚糖:大豆分离蛋白(w/w)=2:1,PH=7,交联剂使用量为4%干粉重,反应温度60℃,反应时间为2h。膜的拉伸强度为34.56MPa,断裂伸长为20.85%,吸湿增量为7.25%,透湿系数为1.64×10-9g·cm/cm2·s·Pa,试验表明,改性产物膜性能较魔芋葡甘聚糖膜、氧化葡甘聚糖膜有较大改善,适于作为空心胶囊囊材。
3、对氧化魔芋葡甘聚糖-大豆分离蛋白进行表征。运用流变仪、红外光谱、扫描电镜、差示扫描量热仪表征改性材料的结构及形态。结果表明,氧化葡甘聚糖与大豆分离蛋白在交联剂作用下发生反应生成新的物质,且改性产物的溶胶流变特性较魔芋葡甘聚糖、氧化魔芋葡甘聚糖都有较大提高。材料的成膜性、流变性和热稳定性都明显改善,符合作为空心胶囊囊材的要求。
4、对氧化葡甘聚糖-大豆分离蛋白进行急性毒性试验,LD50>3.6 g /kg BW,初步判断该改性产物是安全的。
5、以氧化魔芋葡甘聚糖-大豆分离蛋白为原料,辅以助凝剂卡拉胶,按传统的工艺方法制备出植物空心胶囊。依照中国药典对胶囊的性能进行检测,均符合药典标准。
6、检测了胶囊的稳定性。通过高温试验,吸湿性试验证明胶囊具有一定的稳定性。其吸湿性明显低于现有明胶空心胶囊。
The studies on property of natural konjac glucomannan(KGM) and soy protein isolate (SPI) from chemical modification were carried out in this thesis.It was used for the material of vacant capsules.Main research results were as follows:
1. The oxidization conditions of konjac glucomannan were investigated .The viscosity of OKGM gum and film properties of mechanics, moisture resistance and gas barrier were tested. The results indicated that the optimal condition were as follows:2.4% (v/v)H2O2, at 50℃and 5 hours reaction.The tensile strength and elongation rate of oxidized konjac glucomannan (OKGM) film were 15.38MPa and 18.7% respectively, and the moisture absorption of OKGM film was 23.41% ,humidity-penetrate coefficient was 9.0×10-9g·cm/cm2·s·Pa. Molecular chain was degradated and the molecular weight was reduced after oxidization.The weight-average molecular weight(MW) of OKGM was tested by using the laser light scattering.It was 2.889×105g/mol which reduced 70.5% compared with KGM.
2. The modified film was prepared from oxidized konjac glucomannan and soy protein isolate.The optimal conditions were as follow:OKGM:SPI(w/w) = 2:1, PH=7, 4% sodium polyphosphate, at 60℃and 2 hours reaction.The tensile strength of the film was 34.56Mpa,The elongation rate of blend film was 20.85%,The moisture absorption of blend film was 7.25% and the humidity-penetrate coefficient of blend film was 1.64×10-9g·cm/cm2·s·Pa。The properties of film were improved markedly compared with KGM and OKGM film ,which was suitable for materials as vacant capsules.
3. The structure of the modified films was characterized by using rheometer ,Fourier transform infrared spectrum(FT-IR),scanning electron microscope(SEM),and differential scanning calorimeter(DSC) The results indicated that there were strong interactions between OKGM and SPI, OKGM and SPI can be crosslinked to form new material. Film-forming, rheology and thermal stability of the material are markedly improved, which was feasible for vacant capsules materials.
4. The acute oral toxicity of OKGM-SPI was tested, LD50>3.6g /kg BW , the product was safe.
5. The quality of the capsules made from OKGM-SPI was tested based on Chinese Pharmacopoeia, it was qualified.
6. The moisture absorption and stability of capsules were investigated. The results indicated that capsule was stable. Moisture absorption was significantly lower than its existing gelatin capsules.
1、对魔芋葡甘聚糖进行氧化改性,并测定溶胶的粘度和成膜后膜的力学性能、阻湿性能和阻气性能等指标。结果表明,氧化最佳反应条件为:2.4%H2O2(v/v),50℃反应5h,膜的拉伸强度为15.38MPa,断裂伸长率为18.7%,吸湿增重为23.41%,透湿系数为9.0×10-9g·cm/cm2·s·Pa,膜的综合性能较KGM有较大提高。氧化后分子链断裂,分子量降低。通过激光光散射检测其重均分子量为2.889×105 g/mol,较KGM下降了70.5%。
2、对氧化魔芋葡甘聚糖与大豆分离蛋白进行交联改性,制备出一种新的材料氧化魔芋葡甘聚糖-大豆分离蛋白,确定最佳反应条件为:氧化魔芋葡甘聚糖:大豆分离蛋白(w/w)=2:1,PH=7,交联剂使用量为4%干粉重,反应温度60℃,反应时间为2h。膜的拉伸强度为34.56MPa,断裂伸长为20.85%,吸湿增量为7.25%,透湿系数为1.64×10-9g·cm/cm2·s·Pa,试验表明,改性产物膜性能较魔芋葡甘聚糖膜、氧化葡甘聚糖膜有较大改善,适于作为空心胶囊囊材。
3、对氧化魔芋葡甘聚糖-大豆分离蛋白进行表征。运用流变仪、红外光谱、扫描电镜、差示扫描量热仪表征改性材料的结构及形态。结果表明,氧化葡甘聚糖与大豆分离蛋白在交联剂作用下发生反应生成新的物质,且改性产物的溶胶流变特性较魔芋葡甘聚糖、氧化魔芋葡甘聚糖都有较大提高。材料的成膜性、流变性和热稳定性都明显改善,符合作为空心胶囊囊材的要求。
4、对氧化葡甘聚糖-大豆分离蛋白进行急性毒性试验,LD50>3.6 g /kg BW,初步判断该改性产物是安全的。
5、以氧化魔芋葡甘聚糖-大豆分离蛋白为原料,辅以助凝剂卡拉胶,按传统的工艺方法制备出植物空心胶囊。依照中国药典对胶囊的性能进行检测,均符合药典标准。
6、检测了胶囊的稳定性。通过高温试验,吸湿性试验证明胶囊具有一定的稳定性。其吸湿性明显低于现有明胶空心胶囊。
The studies on property of natural konjac glucomannan(KGM) and soy protein isolate (SPI) from chemical modification were carried out in this thesis.It was used for the material of vacant capsules.Main research results were as follows:
1. The oxidization conditions of konjac glucomannan were investigated .The viscosity of OKGM gum and film properties of mechanics, moisture resistance and gas barrier were tested. The results indicated that the optimal condition were as follows:2.4% (v/v)H2O2, at 50℃and 5 hours reaction.The tensile strength and elongation rate of oxidized konjac glucomannan (OKGM) film were 15.38MPa and 18.7% respectively, and the moisture absorption of OKGM film was 23.41% ,humidity-penetrate coefficient was 9.0×10-9g·cm/cm2·s·Pa. Molecular chain was degradated and the molecular weight was reduced after oxidization.The weight-average molecular weight(MW) of OKGM was tested by using the laser light scattering.It was 2.889×105g/mol which reduced 70.5% compared with KGM.
2. The modified film was prepared from oxidized konjac glucomannan and soy protein isolate.The optimal conditions were as follow:OKGM:SPI(w/w) = 2:1, PH=7, 4% sodium polyphosphate, at 60℃and 2 hours reaction.The tensile strength of the film was 34.56Mpa,The elongation rate of blend film was 20.85%,The moisture absorption of blend film was 7.25% and the humidity-penetrate coefficient of blend film was 1.64×10-9g·cm/cm2·s·Pa。The properties of film were improved markedly compared with KGM and OKGM film ,which was suitable for materials as vacant capsules.
3. The structure of the modified films was characterized by using rheometer ,Fourier transform infrared spectrum(FT-IR),scanning electron microscope(SEM),and differential scanning calorimeter(DSC) The results indicated that there were strong interactions between OKGM and SPI, OKGM and SPI can be crosslinked to form new material. Film-forming, rheology and thermal stability of the material are markedly improved, which was feasible for vacant capsules materials.
4. The acute oral toxicity of OKGM-SPI was tested, LD50>3.6g /kg BW , the product was safe.
5. The quality of the capsules made from OKGM-SPI was tested based on Chinese Pharmacopoeia, it was qualified.
6. The moisture absorption and stability of capsules were investigated. The results indicated that capsule was stable. Moisture absorption was significantly lower than its existing gelatin capsules.
引文
[1]张俐娜.天然高分子改性材料及应用[M].北京:化学工业出版社,2006:3-4.
[2]庞杰,林琼,张甫生等.魔芋葡甘聚糖功能材料研究与应用进展[J].结构化学,2003,22(11):633-642.
[3] Zhou Gang,Li Yubao,Zhang Li.The Study of Tri-phasic Iinteractions in Nano-hydroxyapatit Konjac Glucomannan/chitosan Composite[J]. Sci (2007) 42:2591-2597.
[4]LI Bin,XIE Bi-jun.Study on Molecular Chain Morphology of Konjac Glucomannan[J].Agricultural Science in China,2003,2(7):798-803.
[5] B.Li,B.J.Xie..Single Molecular Chain geometry of Konjac Glucomannan as a high quality dietary fiber in East Asia[J].Food Research International,2006(39):127-132.
[6] M.J.Gidley, A.J.McArthur, D.R.Clmolerivood[J].Food Hydrocolloidds, 1991,5,129.
[7]吴绍艳等.魔芋葡甘聚糖纯化及改性研究[D硕士学位论文].华中师范大学.
[8]许时婴,钱和.魔芋葡甘聚糖的化学结构与流变性质[J].无锡轻工业学院学报,1991,10(1): 1-11.
[9]詹小卉,张小菊,李万芬等.羧甲基魔芋葡甘聚糖/大豆蛋白复合物膜及性能研究.食品科学,2007,28(1):39-42.
[10] Jiang fatang,Liwanfen,ZHAN Xiaohui,CHEN Guofeng.Preparation and Characterization of Konjac Superabsorbent Polymer[J].Journal of WuHan University of Technology-Mater.Sci.Ed.2006,21(4):87~91.
[11]李斌,谢笔均等.魔芋葡甘聚糖的H2O2氧化改性及其流变性能研究[J].湖北化工,2002,19(1): 9-11.
[12]朱文均等.魔芋葡甘聚糖的化学改性及其应用性能的研究[J].苏州大学学报[J].1999,15(1):81-86.
[13]胡耀星,谢笔均.魔芋葡甘聚糖马来酸酐酯化反应的研究[J].食品科学,1994,4,5-10.
[14]李斌,汪超.脱乙酰基对魔芋葡甘聚糖羧甲基改性的影响及产物结构表征[J].中国农业科学,2006,38(10):2090-2095.
[15]罗学刚.高强度可食性魔芋葡甘聚糖薄膜研究[J].中国包装,2000,20(1):89-91.
[16]刘惠君.胡慰望.魔芋精粉与丙烯酸丁酯接枝共聚反应及其产物对柑涂膜的保鲜研究[J] .食品科学,1998,19(11):50-52.
[17]李万芬、汪超、姜发堂等.魔芋葡甘聚糖-丙稀酸接枝共聚物的吸湿特性研究[J].农业工程学报,2006, 22(11):228-231.
[18]孙远明,吴青等.魔芋KGM的结构、食品学性质及保健功能[J].食品与发酵工业,1999,25 (5):47-51.
[19]陈昀,赵谋明等.蛋白质与多糖交互作用研究进展[J].食品科学,2001,22(4):90-93.
[20]李斌.高强度魔芋葡甘聚糖及其衍生物-无机复合材料、结构与性能研究.[D].武汉,华中农业大学,2002.
[21]Hsu S.Rheological studies on gelling behavior of soy protein isolates.journal of Food Science,1999,64(1):136-140.
[22]迟玉杰,朱秀清,李文滨等.大豆蛋白质加工新技术[M].北京:科学出版社,第一版,2008,5.
[23]夏友富.我国大豆产业发展战略研究[J].管理世界2003, (3), 96.
[24] Ryu H.S,Rhee K.C., Lee K.H. Protein solubility characteristics of commercial soy protein products[J]. J Am Oil Cehm Soc 2003, 80, 85.
[25] Mizuno A,Mitsuiki M., Motoki M. Effect of transglutaminase treatment on the glass transition of oy protein. J Agric Food Chem 2000, 48, 3286.
[26] Ortiz S.E.M, Cristina A.M. Analysis of products, mechanisms of reaction, and some functional properties of soy protein hydrolysates. J Am Oil Chem Soc 2000, 77, 1293.
[27]周瑞宝,周兵.大豆7s和11S球蛋白的结构和功能性质.中国粮油学报1998, 13(6), 39.
[28]石彦国.任莉.大豆制品工艺学[M].北京:中国轻工业出版社, 1998.
[29] Mananbe M, Bull Chem. Soc. Jpn,1992, 2319-2323.
[30] Wu YV, Inglet G.E. Denaturation of plant protein related to functonality and food applications. A review. J Food Sci 1974, 39, 218.
[31]姚穆,来侃,孙润军,蒋素掸.大豆和大豆蛋白质组成与结构的研究[J].棉纺织技术2002 30(9), 545.
[32]陈海敏,华欲飞.大豆蛋白组成与功能关系研究[J].西部粮油科技,2001,26(3):36-38.
[33] Hermansson A.M. Soy protein gelation. J Am Oil Chem Soc 1986, 63, 658.
[34]黄友如,华欲飞,裘爱泳.大豆分离蛋白功能性质及其影响因素[J].粮油与油脂,2003,5:12-15.
[35]黄曼,卞科.交联剂对大豆蛋白疏水性的影响[J].郑州工程学院学报,2002,23(2),5.
[36] Kapian D.L. (Ed.) Biopolymers from Renewable Resources. Springer-Verlag Berlin Heidelberg,Germany. 1998. p145-176.
[37]刘天一.大豆蛋白的磷酸化改性及功能性质的研究[J].食品发酵工业,2004,10(3),118-120.
[38]奚冬雷.大豆蛋白接枝苯乙烯的合成及膜性能.化学研究与应用[J].2005, 17(4),523-524.
[39]邓扬悟,田少君.大豆分离蛋白的成膜性研究[J].郑州工程学院学报2004,25(2),17-21.
[40]姚静玉,杨晓泉.酰化对大豆分离蛋白水合性质的影响药[J].食品与机械,2005, 22(4),17-20.
[41]李亚琴,周建平.药物制剂工程[M].北京:化学工业出版社,2008.
[42]张劲.药物制剂技术[M].第一版.北京:化学工业出版社,2005,8:156.
[43]曲歌.国家药监局要求加强牛源性药品监管[A].2002. Available via http://www.cctv.com.
[44]陆彬.药剂学[M].北京:中国医药科技出版社,2003.
[45] Yang; Joo Hwan (Bucheon,KR).Cellulose capsule using mixed solution of pectin and the manufacturing process thereof[P].US:6 410 050,2000-06-12.
[46]侯惠民,王浩,张光杰.药用辅料应用技术(第二版)[M].北京:中国医药科技出版社,2002.91.
[47]全球最先进的胶囊工厂落户中国.中国中医药报,第001版,2005,11,4.
[48]徐铮奎.非明胶胶囊外壳原料研究开发新进展[J].中国制药信息,2001,17(6):19-20.
[49]日本用茁霉多糖制成植物性硬空心胶囊[J].四川食品与发酵,2003,39(117):35.
[50]青岛医药科学研究所:海洋生物胶制备药用胶囊(内部资料),1982.
[51]庄绍冰,黄启权.白及空心胶囊及其质量标准[J].中国药学杂志1995,30(3):173.
[52]郑芳.海带胶空心胶囊沾胶机的研制[J].农机与食品机械1999,262(4):29-30.
[53]我国从海藻中成功提取空心胶囊[J].食品与发酵工业,2005,31(6):46.
[54]吴国庆.植物硬胶囊及其生产工艺[P].中国:1 292 261,2001-04-25.
[55]刘杰,高玉宁.硬质药物胶囊[P].中国:100998572,2007-07-18.
[56]郑建璋.植物空心胶囊的制备方法[P].中国:1931145,2006-03-21.
[57]张小菊,姜发堂.羧甲基魔芋葡甘聚糖的制备及应用于空心胶囊囊材的研究[J].食品科学.2004,25(10):200-203.
[58]高俊起,李玉宏.一种药用的空心胶囊壳的制造方法[P].中国:1 565 411,2005-01-19.
[59]陈运中.一种高性能魔芋复合空心胶囊及制备方法[P].中国:1 739 488,2006-03-01.
[60]郑化,杜予民,周金平,张俐娜.纤维素/甲壳素共混膜的结构表征与抗凝血性能[J].高分子学报.2002(4):525-529.
[61] Chaobo Xiao, Lihui Weng, Lina Zhang. Improvement of physical properties of crosslinked alginate and carboxymethyl konjac glucomannan blend films[J]. Journal of Applied Polymer Science, 2002, 84(13):2554-2560.
[62] Kato K. Preparation and functional properties of protein–polysaccharide conjugate. Surface Activity of Proteins Chemical and Physicochemical Modifications[J].New York: Shlomo Magdassi, Marcel Dekker, Ine, 1996:115-130.
[63] SHU Y W, SAHARAS,NAKAMURAS,eta. Effects of the length of polysaccharide chains on the functional properties of the Maillard-type lysozyme-polysaccharide conjugate [J]. J Agric Food Chem, 1996, 44:2544.
[64] Rodriguez-Patino J.M., Molina-Ortiz S.E.,Sanchez C.C., et..Behavior of Soy Globulin Films at the Air-Water Interface, Structure and Dilatational Properties of Spread Films[J]. Industrial & Engineering Chemistry Research,2003,42(21):5011-5017.
[65]何东保,詹东风,张文举.魔芋精粉与黄原胶协同相互作用及其凝胶化的研究[J].高分子学报,1999,(4):460-464.
[66]丁金龙,孙远明,乐学义.魔芋胶与大豆分离蛋白相互作用研究[J].中国粮油学报,2003,18(3):65-69,77.
[67]赵谋明,林伟峰.酪蛋白-卡拉胶体系交互作用机理的研究[J].食品与发酵工业,1997,23(5):1-9.
[68]詹小卉,张小菊,姜发堂等.羧甲基葡甘聚糖-大豆分离蛋白复合物膜及性能研究[J].食品科学,2007,28(1):39-42.
[69]马惠玲,张院民.魔芋精粉的物理改性研究[J].食品工业科技,1998,(5):27-28.
[70] HE D B,PEN X D,ZHAN D F.Study on synergistic interaction and gelation of xanthan and amophophalloglucomannan maleic acid ester[J].Journal of Wuhan University,2000,46(6),681.
[71]B.Li,,J.F.Kennedy,Q.G.Jiang,B,J,Xie.Quick dissolvable,edible and heatsealable blend filmsbased on konjac glucomannan-Gelatin.Food Research International[J].2006,39:544 -549.
[72]张小菊,姜发堂.羧甲基葡甘聚糖的制备[J].化学与生物工程,2004(1):25-28.
[73]刘爱红,姜发堂,张声华.魔芋粉接枝丙烯酸(钠)超强吸水剂的制备[J].材料科学与工程学报,2004,22(4):588-591.
[74]李万芬,汪超,姜发堂.魔芋葡甘聚糖接枝丙烯酸共聚物超强吸湿剂的扩散吸湿特性研究[J].材料科学与工程学报.2007,25(2):276-300.
[75] Jiang fatang,Liwanfen,ZHAN Xiaohui,CHEN Guofeng.Preparation and Characterization of Konjac Superabsorbent Polymer[J]. Journal of WuHan University of Technology-Mater.Sci.Ed.2006,21(4):87-91.
[76]祁黎,李光吉,宗敏华.酶催化魔芋葡甘聚糖的可控降解[J].高分子学报,2003,(5):650-654.
[77]汪超,李斌.魔芋葡甘聚糖的流变特性研究[J].农业工程学报,2005,21(8):157-160.
[78] GB/T 16421-1996,塑料拉伸性能小试样试验方法[S].
[79]詹小卉,陈国锋.大豆蛋白和羧甲基葡甘聚糖共混膜的制备和性能[J].食品工业科技,2006,27(8):144-145,159.
[80] GB/T 1037-1988,塑料薄膜和片材透水蒸气性试验方法[S].
[81]张俐娜,薛奇,莫志深,金熹高.高分子物理近代研究方法[M].第二版.武汉:武汉大学出版社, 2006:313.
[82]黄曼,卞科.交联剂对大豆蛋白疏水性的影响[J].郑州工程学院学报,2002,23(2),5.
[83] Sanchez C.C., Ortiz S.E.M., NiHo M.R.R., Effect of pH on structural, topographical, and dynamic characteristics of soy globulin films at the air-water interface[J]. Langmuir2003,19, 7478.
[84] Nishinari, K., Zhang, H., & Ikeda, S. Hydrocolloid gels of polysaccharides and proteins[J]. Current Opinion in Colloid & Interface Science, 2002; 5: 195-201.
[85] Chu, B., Laser Light Scattering[J], Academic Press, New York,1974.
[86] Tada, Y., Matsumoto, T., &Masuda, T. Dynamic xiscoelasticity and small-angle X-ray scattering studies on the gelation mechanism and network structure of curdlan gels[J]. Carbohydrate Polymer, 1999; 39: 53-59.
[87] Geissler., E. Himadri. B., Anne2Marie Hechi. Macro molecules, 1985, 18 (5): 949.
[88] H.A.巴赫斯,J.H.赫顿,K.瓦尔特斯著.吴大诚译.流变学导引[M].北京:中国石化出版社,1992.
[89]许元泽著.高分子结构流变学[M].四川:四川教育出版社,1988.
[90]侯新朴.物理化学[M].第六版.北京:人民卫生出版社,2007,7:348.
[91]《化学药物急性毒性试验技术指导原则》课题研究组编著.《化学药物急性毒性研究技术指导原则》,2005.
[92]孙积乾主编.卫生统计学.北京:人民卫生出版社,2003.
[93]空心胶囊药典标准[S].中国药典,2000版二部-438.
[94]国家医药管理局.药品检验操作规程,第8部分,制剂通则.1996,148.
[95]朱瑞英.影响胶囊剂崩解时限的研究[J].苏州医学院学报,1998,18(2):132.
[96]张劲.药物制剂技术[M].第一版.北京:化学工业出版社,2005,8:308-309.
[2]庞杰,林琼,张甫生等.魔芋葡甘聚糖功能材料研究与应用进展[J].结构化学,2003,22(11):633-642.
[3] Zhou Gang,Li Yubao,Zhang Li.The Study of Tri-phasic Iinteractions in Nano-hydroxyapatit Konjac Glucomannan/chitosan Composite[J]. Sci (2007) 42:2591-2597.
[4]LI Bin,XIE Bi-jun.Study on Molecular Chain Morphology of Konjac Glucomannan[J].Agricultural Science in China,2003,2(7):798-803.
[5] B.Li,B.J.Xie..Single Molecular Chain geometry of Konjac Glucomannan as a high quality dietary fiber in East Asia[J].Food Research International,2006(39):127-132.
[6] M.J.Gidley, A.J.McArthur, D.R.Clmolerivood[J].Food Hydrocolloidds, 1991,5,129.
[7]吴绍艳等.魔芋葡甘聚糖纯化及改性研究[D硕士学位论文].华中师范大学.
[8]许时婴,钱和.魔芋葡甘聚糖的化学结构与流变性质[J].无锡轻工业学院学报,1991,10(1): 1-11.
[9]詹小卉,张小菊,李万芬等.羧甲基魔芋葡甘聚糖/大豆蛋白复合物膜及性能研究.食品科学,2007,28(1):39-42.
[10] Jiang fatang,Liwanfen,ZHAN Xiaohui,CHEN Guofeng.Preparation and Characterization of Konjac Superabsorbent Polymer[J].Journal of WuHan University of Technology-Mater.Sci.Ed.2006,21(4):87~91.
[11]李斌,谢笔均等.魔芋葡甘聚糖的H2O2氧化改性及其流变性能研究[J].湖北化工,2002,19(1): 9-11.
[12]朱文均等.魔芋葡甘聚糖的化学改性及其应用性能的研究[J].苏州大学学报[J].1999,15(1):81-86.
[13]胡耀星,谢笔均.魔芋葡甘聚糖马来酸酐酯化反应的研究[J].食品科学,1994,4,5-10.
[14]李斌,汪超.脱乙酰基对魔芋葡甘聚糖羧甲基改性的影响及产物结构表征[J].中国农业科学,2006,38(10):2090-2095.
[15]罗学刚.高强度可食性魔芋葡甘聚糖薄膜研究[J].中国包装,2000,20(1):89-91.
[16]刘惠君.胡慰望.魔芋精粉与丙烯酸丁酯接枝共聚反应及其产物对柑涂膜的保鲜研究[J] .食品科学,1998,19(11):50-52.
[17]李万芬、汪超、姜发堂等.魔芋葡甘聚糖-丙稀酸接枝共聚物的吸湿特性研究[J].农业工程学报,2006, 22(11):228-231.
[18]孙远明,吴青等.魔芋KGM的结构、食品学性质及保健功能[J].食品与发酵工业,1999,25 (5):47-51.
[19]陈昀,赵谋明等.蛋白质与多糖交互作用研究进展[J].食品科学,2001,22(4):90-93.
[20]李斌.高强度魔芋葡甘聚糖及其衍生物-无机复合材料、结构与性能研究.[D].武汉,华中农业大学,2002.
[21]Hsu S.Rheological studies on gelling behavior of soy protein isolates.journal of Food Science,1999,64(1):136-140.
[22]迟玉杰,朱秀清,李文滨等.大豆蛋白质加工新技术[M].北京:科学出版社,第一版,2008,5.
[23]夏友富.我国大豆产业发展战略研究[J].管理世界2003, (3), 96.
[24] Ryu H.S,Rhee K.C., Lee K.H. Protein solubility characteristics of commercial soy protein products[J]. J Am Oil Cehm Soc 2003, 80, 85.
[25] Mizuno A,Mitsuiki M., Motoki M. Effect of transglutaminase treatment on the glass transition of oy protein. J Agric Food Chem 2000, 48, 3286.
[26] Ortiz S.E.M, Cristina A.M. Analysis of products, mechanisms of reaction, and some functional properties of soy protein hydrolysates. J Am Oil Chem Soc 2000, 77, 1293.
[27]周瑞宝,周兵.大豆7s和11S球蛋白的结构和功能性质.中国粮油学报1998, 13(6), 39.
[28]石彦国.任莉.大豆制品工艺学[M].北京:中国轻工业出版社, 1998.
[29] Mananbe M, Bull Chem. Soc. Jpn,1992, 2319-2323.
[30] Wu YV, Inglet G.E. Denaturation of plant protein related to functonality and food applications. A review. J Food Sci 1974, 39, 218.
[31]姚穆,来侃,孙润军,蒋素掸.大豆和大豆蛋白质组成与结构的研究[J].棉纺织技术2002 30(9), 545.
[32]陈海敏,华欲飞.大豆蛋白组成与功能关系研究[J].西部粮油科技,2001,26(3):36-38.
[33] Hermansson A.M. Soy protein gelation. J Am Oil Chem Soc 1986, 63, 658.
[34]黄友如,华欲飞,裘爱泳.大豆分离蛋白功能性质及其影响因素[J].粮油与油脂,2003,5:12-15.
[35]黄曼,卞科.交联剂对大豆蛋白疏水性的影响[J].郑州工程学院学报,2002,23(2),5.
[36] Kapian D.L. (Ed.) Biopolymers from Renewable Resources. Springer-Verlag Berlin Heidelberg,Germany. 1998. p145-176.
[37]刘天一.大豆蛋白的磷酸化改性及功能性质的研究[J].食品发酵工业,2004,10(3),118-120.
[38]奚冬雷.大豆蛋白接枝苯乙烯的合成及膜性能.化学研究与应用[J].2005, 17(4),523-524.
[39]邓扬悟,田少君.大豆分离蛋白的成膜性研究[J].郑州工程学院学报2004,25(2),17-21.
[40]姚静玉,杨晓泉.酰化对大豆分离蛋白水合性质的影响药[J].食品与机械,2005, 22(4),17-20.
[41]李亚琴,周建平.药物制剂工程[M].北京:化学工业出版社,2008.
[42]张劲.药物制剂技术[M].第一版.北京:化学工业出版社,2005,8:156.
[43]曲歌.国家药监局要求加强牛源性药品监管[A].2002. Available via http://www.cctv.com.
[44]陆彬.药剂学[M].北京:中国医药科技出版社,2003.
[45] Yang; Joo Hwan (Bucheon,KR).Cellulose capsule using mixed solution of pectin and the manufacturing process thereof[P].US:6 410 050,2000-06-12.
[46]侯惠民,王浩,张光杰.药用辅料应用技术(第二版)[M].北京:中国医药科技出版社,2002.91.
[47]全球最先进的胶囊工厂落户中国.中国中医药报,第001版,2005,11,4.
[48]徐铮奎.非明胶胶囊外壳原料研究开发新进展[J].中国制药信息,2001,17(6):19-20.
[49]日本用茁霉多糖制成植物性硬空心胶囊[J].四川食品与发酵,2003,39(117):35.
[50]青岛医药科学研究所:海洋生物胶制备药用胶囊(内部资料),1982.
[51]庄绍冰,黄启权.白及空心胶囊及其质量标准[J].中国药学杂志1995,30(3):173.
[52]郑芳.海带胶空心胶囊沾胶机的研制[J].农机与食品机械1999,262(4):29-30.
[53]我国从海藻中成功提取空心胶囊[J].食品与发酵工业,2005,31(6):46.
[54]吴国庆.植物硬胶囊及其生产工艺[P].中国:1 292 261,2001-04-25.
[55]刘杰,高玉宁.硬质药物胶囊[P].中国:100998572,2007-07-18.
[56]郑建璋.植物空心胶囊的制备方法[P].中国:1931145,2006-03-21.
[57]张小菊,姜发堂.羧甲基魔芋葡甘聚糖的制备及应用于空心胶囊囊材的研究[J].食品科学.2004,25(10):200-203.
[58]高俊起,李玉宏.一种药用的空心胶囊壳的制造方法[P].中国:1 565 411,2005-01-19.
[59]陈运中.一种高性能魔芋复合空心胶囊及制备方法[P].中国:1 739 488,2006-03-01.
[60]郑化,杜予民,周金平,张俐娜.纤维素/甲壳素共混膜的结构表征与抗凝血性能[J].高分子学报.2002(4):525-529.
[61] Chaobo Xiao, Lihui Weng, Lina Zhang. Improvement of physical properties of crosslinked alginate and carboxymethyl konjac glucomannan blend films[J]. Journal of Applied Polymer Science, 2002, 84(13):2554-2560.
[62] Kato K. Preparation and functional properties of protein–polysaccharide conjugate. Surface Activity of Proteins Chemical and Physicochemical Modifications[J].New York: Shlomo Magdassi, Marcel Dekker, Ine, 1996:115-130.
[63] SHU Y W, SAHARAS,NAKAMURAS,eta. Effects of the length of polysaccharide chains on the functional properties of the Maillard-type lysozyme-polysaccharide conjugate [J]. J Agric Food Chem, 1996, 44:2544.
[64] Rodriguez-Patino J.M., Molina-Ortiz S.E.,Sanchez C.C., et..Behavior of Soy Globulin Films at the Air-Water Interface, Structure and Dilatational Properties of Spread Films[J]. Industrial & Engineering Chemistry Research,2003,42(21):5011-5017.
[65]何东保,詹东风,张文举.魔芋精粉与黄原胶协同相互作用及其凝胶化的研究[J].高分子学报,1999,(4):460-464.
[66]丁金龙,孙远明,乐学义.魔芋胶与大豆分离蛋白相互作用研究[J].中国粮油学报,2003,18(3):65-69,77.
[67]赵谋明,林伟峰.酪蛋白-卡拉胶体系交互作用机理的研究[J].食品与发酵工业,1997,23(5):1-9.
[68]詹小卉,张小菊,姜发堂等.羧甲基葡甘聚糖-大豆分离蛋白复合物膜及性能研究[J].食品科学,2007,28(1):39-42.
[69]马惠玲,张院民.魔芋精粉的物理改性研究[J].食品工业科技,1998,(5):27-28.
[70] HE D B,PEN X D,ZHAN D F.Study on synergistic interaction and gelation of xanthan and amophophalloglucomannan maleic acid ester[J].Journal of Wuhan University,2000,46(6),681.
[71]B.Li,,J.F.Kennedy,Q.G.Jiang,B,J,Xie.Quick dissolvable,edible and heatsealable blend filmsbased on konjac glucomannan-Gelatin.Food Research International[J].2006,39:544 -549.
[72]张小菊,姜发堂.羧甲基葡甘聚糖的制备[J].化学与生物工程,2004(1):25-28.
[73]刘爱红,姜发堂,张声华.魔芋粉接枝丙烯酸(钠)超强吸水剂的制备[J].材料科学与工程学报,2004,22(4):588-591.
[74]李万芬,汪超,姜发堂.魔芋葡甘聚糖接枝丙烯酸共聚物超强吸湿剂的扩散吸湿特性研究[J].材料科学与工程学报.2007,25(2):276-300.
[75] Jiang fatang,Liwanfen,ZHAN Xiaohui,CHEN Guofeng.Preparation and Characterization of Konjac Superabsorbent Polymer[J]. Journal of WuHan University of Technology-Mater.Sci.Ed.2006,21(4):87-91.
[76]祁黎,李光吉,宗敏华.酶催化魔芋葡甘聚糖的可控降解[J].高分子学报,2003,(5):650-654.
[77]汪超,李斌.魔芋葡甘聚糖的流变特性研究[J].农业工程学报,2005,21(8):157-160.
[78] GB/T 16421-1996,塑料拉伸性能小试样试验方法[S].
[79]詹小卉,陈国锋.大豆蛋白和羧甲基葡甘聚糖共混膜的制备和性能[J].食品工业科技,2006,27(8):144-145,159.
[80] GB/T 1037-1988,塑料薄膜和片材透水蒸气性试验方法[S].
[81]张俐娜,薛奇,莫志深,金熹高.高分子物理近代研究方法[M].第二版.武汉:武汉大学出版社, 2006:313.
[82]黄曼,卞科.交联剂对大豆蛋白疏水性的影响[J].郑州工程学院学报,2002,23(2),5.
[83] Sanchez C.C., Ortiz S.E.M., NiHo M.R.R., Effect of pH on structural, topographical, and dynamic characteristics of soy globulin films at the air-water interface[J]. Langmuir2003,19, 7478.
[84] Nishinari, K., Zhang, H., & Ikeda, S. Hydrocolloid gels of polysaccharides and proteins[J]. Current Opinion in Colloid & Interface Science, 2002; 5: 195-201.
[85] Chu, B., Laser Light Scattering[J], Academic Press, New York,1974.
[86] Tada, Y., Matsumoto, T., &Masuda, T. Dynamic xiscoelasticity and small-angle X-ray scattering studies on the gelation mechanism and network structure of curdlan gels[J]. Carbohydrate Polymer, 1999; 39: 53-59.
[87] Geissler., E. Himadri. B., Anne2Marie Hechi. Macro molecules, 1985, 18 (5): 949.
[88] H.A.巴赫斯,J.H.赫顿,K.瓦尔特斯著.吴大诚译.流变学导引[M].北京:中国石化出版社,1992.
[89]许元泽著.高分子结构流变学[M].四川:四川教育出版社,1988.
[90]侯新朴.物理化学[M].第六版.北京:人民卫生出版社,2007,7:348.
[91]《化学药物急性毒性试验技术指导原则》课题研究组编著.《化学药物急性毒性研究技术指导原则》,2005.
[92]孙积乾主编.卫生统计学.北京:人民卫生出版社,2003.
[93]空心胶囊药典标准[S].中国药典,2000版二部-438.
[94]国家医药管理局.药品检验操作规程,第8部分,制剂通则.1996,148.
[95]朱瑞英.影响胶囊剂崩解时限的研究[J].苏州医学院学报,1998,18(2):132.
[96]张劲.药物制剂技术[M].第一版.北京:化学工业出版社,2005,8:308-309.