中草药复方添加剂对繁殖后期种公鸡繁殖性能和血液生理生化指标的影响
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摘要
本试验采用单因子完全随机化设计,研究了日粮中添加不同剂量的中草药复方对繁殖后期尼克T种公鸡繁殖性能和血液生理生化指标的影响。
选用80只50周龄尼克T种公鸡随机分成Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组,每组16只。Ⅰ、Ⅱ组为对照组,分别饲喂基础日粮和基础日粮添加鸡蛋,Ⅲ、Ⅳ、Ⅴ组为试验组,分别饲喂添加水平为1%、2%、3%中草药复方添加剂的日粮。分别在预饲期末和试验期的第15、30、45、60天及试验结束后15 d分别对种公鸡进行精液品质测定;分别在预饲期末和试验期的第30、60天及试验结束后15 d测定血浆生殖激素T、LH和FSH浓度;在试验60 d时测定血清TP、ALB、GLOB、A/G、TC、TG3、HDL、LDL、BUN、UA、的浓度以及GPT和GOT的活性;在试验最后一周对每组种公鸡进行采精,进行人工授精并收集种蛋孵化,测定种蛋受精率和健雏率。研究了中草药复方添加剂对繁殖后期种公鸡精液品质、血浆生殖激素浓度、血清生化指标及种蛋受精率与健雏率的影响。
结果表明:日粮中添加中草药复方添加剂能显著提高种公鸡的精液量和精子活力及血浆中睾酮、促黄体素、促卵泡素浓度,并降低精子畸形率;精液量和精子活力、精子密度、血浆T、LH、FSH浓度呈显著正相关(P<0.05);精子活力和血浆T、LH、FSH浓度呈显著正相关(P<0.05);血浆T浓度和血浆LH、FSH浓度呈显著正相关(P<0.05)。
与Ⅰ组相比,各试验组TP含量分别提高了16.68%( P<0.05)、21.81%(P<0.01)和16.51(P<0.05),GLOB含量分别提高了22.86%、31.13和20.99%(P<0.01),GPT活性分别降低了27.21%、36.62%和24.68%(P<0.01),UA含量分别降低了17.06%、23.05%和18.62%(P<0.01),Ⅳ组公鸡血清LDL显著低于Ⅰ组(P<0.05),其他各组间差异不显著(P>0.05);与Ⅱ组相比,各试验组TP含量分别提高了2.09%(P>0.05)、7.37%(P<0.05)和1.94%(P>0.05),GLOB含量差异不显著(P>0.05),Ⅳ组GPT活性极显著低于对照Ⅱ组(P<0.01),Ⅲ和Ⅴ组显著低于对照Ⅱ组(P<0.05),UA含量分别降低了18.54%、24.42%、20.08%(P<0.01),Ⅳ组血清TC含量显著低于Ⅱ组(P<0.05)。中草药复方能显著降低种公鸡血清GOT活性(P<0.05),对血清BUN有降低的趋势,但差异不显著(P>0.05);对血清HDL有升高的趋势,但差异不显著(P>0.05)。
Ⅲ、Ⅳ和Ⅴ组受精率分别比Ⅰ组提高了5.5%、7.5%和5.0%,Ⅲ和Ⅴ组受精率与Ⅰ组相比差异显著(P<0.05),和Ⅱ组相比差异不显著(P>0.05),Ⅴ组受精率与Ⅲ和Ⅴ组相比差异不显著(P>0.05),显著高于Ⅱ组(P<0.05),极显著高于Ⅰ组(P<0.01),Ⅱ组受精率
This research was conducted to evaluate the effects of Chinese herb feed additives on sperm quality, plasma reproductive hormone, physiological and biochemical parameters during later reproductive stage of Nick T breeder cocks.
80 fifty-week-old Nick T breeder cocks were randomly divided intoⅠ、Ⅱ、Ⅲ、Ⅳ、Ⅴgroups. Every group has 16 cocks.ⅠandⅡgroup were control groups. Two control groups were fed with basic diet and basic diets added eggs respectively.Ⅲ、ⅣandⅤgroup were treatment groups. Three treatment groups were fed with basic diets added with 1%、2%、3% Chinese herb feed additives respectively. The sperm quality of breeder cocks were measured at the end of pre-feeding period, the 15th day、the 30th day、the 45th day、the 60th day in test period and the 15th day after test respectively. The plasma reproductive hormone T、LH、FSH of the breeder cocks were measured at the end of pre-feeding period, the 30th day、the 60th day in test period and the 15th day after test respectively. The concentration of TP、ALB、GLOB、A/G、TC、TG3、HDL、LDL、BUN、UA in blood serum and the activity of GPT and GOT were measured at the 60th day of the text . Semen collection were proceeded for every group cocks in the last week of the test period. Artificial insemination were proceeded for hen, and collected the pedigree eggs for hatching. The rate of fertilization of pedigree eggs and the rate of healthy chickling were measured. This research was conducted to evaluate the effects of Chinese herb feed additives on sperm quality, plasma reproductive hormone, physiological and biochemical parameters, the rate of fertilization of prdigree eggs and the rate of healthy chickling during later reproductive stage of Nick T breeder cocks.
The results showed that: the diets added with Chinese herb feed additives can improve sperm quantity and sperm activity of breeder cocks significantly. The levels of testosterone、
选用80只50周龄尼克T种公鸡随机分成Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组,每组16只。Ⅰ、Ⅱ组为对照组,分别饲喂基础日粮和基础日粮添加鸡蛋,Ⅲ、Ⅳ、Ⅴ组为试验组,分别饲喂添加水平为1%、2%、3%中草药复方添加剂的日粮。分别在预饲期末和试验期的第15、30、45、60天及试验结束后15 d分别对种公鸡进行精液品质测定;分别在预饲期末和试验期的第30、60天及试验结束后15 d测定血浆生殖激素T、LH和FSH浓度;在试验60 d时测定血清TP、ALB、GLOB、A/G、TC、TG3、HDL、LDL、BUN、UA、的浓度以及GPT和GOT的活性;在试验最后一周对每组种公鸡进行采精,进行人工授精并收集种蛋孵化,测定种蛋受精率和健雏率。研究了中草药复方添加剂对繁殖后期种公鸡精液品质、血浆生殖激素浓度、血清生化指标及种蛋受精率与健雏率的影响。
结果表明:日粮中添加中草药复方添加剂能显著提高种公鸡的精液量和精子活力及血浆中睾酮、促黄体素、促卵泡素浓度,并降低精子畸形率;精液量和精子活力、精子密度、血浆T、LH、FSH浓度呈显著正相关(P<0.05);精子活力和血浆T、LH、FSH浓度呈显著正相关(P<0.05);血浆T浓度和血浆LH、FSH浓度呈显著正相关(P<0.05)。
与Ⅰ组相比,各试验组TP含量分别提高了16.68%( P<0.05)、21.81%(P<0.01)和16.51(P<0.05),GLOB含量分别提高了22.86%、31.13和20.99%(P<0.01),GPT活性分别降低了27.21%、36.62%和24.68%(P<0.01),UA含量分别降低了17.06%、23.05%和18.62%(P<0.01),Ⅳ组公鸡血清LDL显著低于Ⅰ组(P<0.05),其他各组间差异不显著(P>0.05);与Ⅱ组相比,各试验组TP含量分别提高了2.09%(P>0.05)、7.37%(P<0.05)和1.94%(P>0.05),GLOB含量差异不显著(P>0.05),Ⅳ组GPT活性极显著低于对照Ⅱ组(P<0.01),Ⅲ和Ⅴ组显著低于对照Ⅱ组(P<0.05),UA含量分别降低了18.54%、24.42%、20.08%(P<0.01),Ⅳ组血清TC含量显著低于Ⅱ组(P<0.05)。中草药复方能显著降低种公鸡血清GOT活性(P<0.05),对血清BUN有降低的趋势,但差异不显著(P>0.05);对血清HDL有升高的趋势,但差异不显著(P>0.05)。
Ⅲ、Ⅳ和Ⅴ组受精率分别比Ⅰ组提高了5.5%、7.5%和5.0%,Ⅲ和Ⅴ组受精率与Ⅰ组相比差异显著(P<0.05),和Ⅱ组相比差异不显著(P>0.05),Ⅴ组受精率与Ⅲ和Ⅴ组相比差异不显著(P>0.05),显著高于Ⅱ组(P<0.05),极显著高于Ⅰ组(P<0.01),Ⅱ组受精率
This research was conducted to evaluate the effects of Chinese herb feed additives on sperm quality, plasma reproductive hormone, physiological and biochemical parameters during later reproductive stage of Nick T breeder cocks.
80 fifty-week-old Nick T breeder cocks were randomly divided intoⅠ、Ⅱ、Ⅲ、Ⅳ、Ⅴgroups. Every group has 16 cocks.ⅠandⅡgroup were control groups. Two control groups were fed with basic diet and basic diets added eggs respectively.Ⅲ、ⅣandⅤgroup were treatment groups. Three treatment groups were fed with basic diets added with 1%、2%、3% Chinese herb feed additives respectively. The sperm quality of breeder cocks were measured at the end of pre-feeding period, the 15th day、the 30th day、the 45th day、the 60th day in test period and the 15th day after test respectively. The plasma reproductive hormone T、LH、FSH of the breeder cocks were measured at the end of pre-feeding period, the 30th day、the 60th day in test period and the 15th day after test respectively. The concentration of TP、ALB、GLOB、A/G、TC、TG3、HDL、LDL、BUN、UA in blood serum and the activity of GPT and GOT were measured at the 60th day of the text . Semen collection were proceeded for every group cocks in the last week of the test period. Artificial insemination were proceeded for hen, and collected the pedigree eggs for hatching. The rate of fertilization of pedigree eggs and the rate of healthy chickling were measured. This research was conducted to evaluate the effects of Chinese herb feed additives on sperm quality, plasma reproductive hormone, physiological and biochemical parameters, the rate of fertilization of prdigree eggs and the rate of healthy chickling during later reproductive stage of Nick T breeder cocks.
The results showed that: the diets added with Chinese herb feed additives can improve sperm quantity and sperm activity of breeder cocks significantly. The levels of testosterone、
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