日粮中添加异黄酮植物雌激素对雏鸡生长性能和甲状腺激素的影响
|
摘要
本文以雏鸡为研究对象,进行了添喂异黄酮植物雌激素(HK90、HK99、HK03和HK04)对雏鸡生长性能影响的研究。主要研究异黄酮植物雌激素对21日龄和35日龄雏鸡生长性能及甲状腺激素水平的影响,以探讨异黄酮植物雌激素对雏鸡的作用机理,为异黄酮植物雌激素在畜牧业生产应用提供一定的理论依据。
1.日粮中添加异黄酮植物雌激素对7-21日龄雏鸡生长性能研究初探
选用7日龄健康雄性雏鸡120只,按初始体重随机分为五组:基础日粮对照组(CON组)、添加5mg/kgHK90组(HK90组)、添加5mg/kgHK99组(HK99组)、添加5mg/kgHK03组(HK03组)、添加5mg/kgHK04组(HK04组)。试验期持续2周。与对照组相比,HK90组、HK99组、HK03组和HK04组料重比有下降的趋势,采食量、日增重、总增重有增加的趋势,但差异不显著(P>0.05)。试验组雏鸡鸡冠、睾丸、肝脏等组织的重量较对照组有增加的趋势,但差异不显著(P>0.05);试验组雏鸡腿肌重量分别较对照组增加了9.88%(P<0.05)、6.89%(P>0.05)、7.49%(P=0.08)和7.49%(P=0.06):腿的重量分别较对照组增加了1519%(P<0.05)、11.79%(P=0.06)、15.42%(P<0.05)和16.78%(P<0.01)。
2.日粮中添加异黄酮植物雌激素对雏鸡生长性能及骨代谢的影响
第一部分 选用7日龄健康雄性雏鸡160只,试验分组同第一章第一部分,试验期持续4周。结果表明,与对照组相比,21日龄时,HK90组、HK99组、HK03组和HK04组每g肌组织RNA含量显著升高(P<0.01),分别较对照组升高了10.98%、12.38%、7.97%和9.04%,总RNA含量分别较对照组提高了23.71%(P<0.05)、16.05%(P>0.05)、21.46%(P<0.05)和28.88%(P<0.05),而DNA含量及总DNA含量无显著变化;35日龄时,每g肌组织中RNA含量较对照组显著提高(P<0.05),分别较对照组提高9.09%、8.91%、7.81%、8.00%,而总RNA含量有升高趋势,与对照组差异不显著,DNA含量无显著变化,而总DNA含量有升高的趋势。21日龄时,血清尿酸含量较对照组下降了16.50%(P<0.05)、16.16%(P<0.05)、15.02%(P<0.05)和21.48%(P<0.01);35日龄时,较对照组减少了9.07%、19.38%、6.14%和17.79%,与对照组差异不显著(P>0.05)。
第二部分 血清样品来自第一部分各组雏鸡。结果表明,21日龄时,与对照组相比,
The effect of isoflavonic phytoestrogens on the growth performance in young chickens and involved thyroid hormones were studied in this study.1. Effect of Diet Supplemented with Isoflavonic phytoestrogens on the Growth Performance in 21-day-old Chickens120 7-day-old male chickens were randomized into 5 groups: basal diet(Con group),basal diet plus 5mg/kg HK90(HK90 group), basal diet plus 5mg/kg HK99(HK99 group), basal diet plus 5mg/kg HKO3(HKO3 group), basal diet plus 5mg/kg HK04(HK04group). Compared with control,the experiment groups increased chickens body weight gain per day,the conversion rate. Compared with control,the experiment groups'tissue weight gains had the tendency of increase,but there were no significant difference;the weight of experiment groups'muscle increased by 9.88 % (P<0.05 )、 6.89 % (P>0.05)、 7.49% (P=0.08) and 7.49% (P=0.06) respectively;the weight of experiment groups' thigh increased by 15.19%(P<0.05)、 11.79%(P=0.06)、 15.42%(P<0.05) and 16.782. Effect of Diet Supplemented with Isoflavonic phytoestrogens on the Growth Performance and the Metabolism of Bone in ChickensPart 1 160 7-day-old male chickens were randomized into 5 groups as chapter 1 part 1.The results indicated were as follows. When they were 21 days old, compared with control, the content of RNA level in per gramme chickens' muscle were increased significantly(P<0.01),increased by 10.98%,12.38%,7.97% and 9.04% respectively in the four experiment groups,the total RNA increased by 23.71%(P<0.05), 16.05%(P>0.05), 21.46% (P<0.05)and 28.88%(P<0.05),but the content of DNA and the total DNA were no
significant difference.The serum uric acid concentration of experiment groups were decreased by 16.50%(P<0.05),16.16%(P<0.05),15.02%(P<0.05) and 21.48%(P<0.01), significantly. When they were 35 days old, compared with control,the content of RNA level in per gramme chickens' muscle were increased significantly(P<0.05),increased by 9.09%, 8.91%,7.81% and 8.00%,the total RNA had the tendency of increase,the content of DNA were similar with control and the total DNA had the tendency of increase. The serum uric acid concentration of experiment groups were decreased by 9.07%, 19.38%,6.14% and 17.79%,but there were no significant difference with control(P>0.05). Part 2 The serum samples came from part 1. When they were 21 days old, compared with control, inorganic phosphorus level were increased by 65.41%(P<0.01), 35.14%(P<0.05), 68.65%(P<0.01) and 94.59%(P<0.01) ,significantly, HK90 group' serum BGP level were increased by 41.53%(P<0.01),the other groups' serum BGP level had the tendency of increase(P>0.05),the AKP activity had the tendency of increase and the calcium level had no significant difference. When they were 35 days old, compared with control,HK99 group' serum calcium level were decreased by 11.19%(P<0.05),HK04 group' inorganic phosphorus were increased by 9.72%(P<0.05),HK99 and HK03 groups' AKP activity were increased by 136.41%(P<0.01) and 108.28%(P<0.01),the other groups' calcium level had the tendency of decrease and inorganic phosphorus,BGP,AKP had the tendency of increase(P>0.05).3. Effect of Diet Supplemented with Isoflavonic phytoestrogens on the Thyroid HormonesThe serum samples came from chapter 2 part l.T3,T4,FT3,FT4 and T were analyzed by radio immunoassay(RIA).When they were 21 days old,compared with control,the serum T3 level were decreased by 19.34%(P<0.05),23.36%(P<0.01),41.24%(P<0.01) and 39.05% (P<0.01) respectively, HK99 group' serum T4 level were increased by 26.91%(P<0.05), HK04 group' serum free T3 level were increased by 46.43%(P<0.01),the other groups' T4 free T3 and free T4 had the tendency of increase(P>0.05).When they were 35 days old, compared with control HK03 and HK04 groups' serum T3 level were decreased by 30.16%(P<0.01) and 22.22%(P<0.05),HK90 group' serum free T3 level were increased by 62.82%(P<0.01),the other groups' serum T3 had the tendency of decrease and free T3 level had the tendency of increase(P>0.05);compared with control.the experiment groups' T4 level had the tendency of increase(P>0.05).
1.日粮中添加异黄酮植物雌激素对7-21日龄雏鸡生长性能研究初探
选用7日龄健康雄性雏鸡120只,按初始体重随机分为五组:基础日粮对照组(CON组)、添加5mg/kgHK90组(HK90组)、添加5mg/kgHK99组(HK99组)、添加5mg/kgHK03组(HK03组)、添加5mg/kgHK04组(HK04组)。试验期持续2周。与对照组相比,HK90组、HK99组、HK03组和HK04组料重比有下降的趋势,采食量、日增重、总增重有增加的趋势,但差异不显著(P>0.05)。试验组雏鸡鸡冠、睾丸、肝脏等组织的重量较对照组有增加的趋势,但差异不显著(P>0.05);试验组雏鸡腿肌重量分别较对照组增加了9.88%(P<0.05)、6.89%(P>0.05)、7.49%(P=0.08)和7.49%(P=0.06):腿的重量分别较对照组增加了1519%(P<0.05)、11.79%(P=0.06)、15.42%(P<0.05)和16.78%(P<0.01)。
2.日粮中添加异黄酮植物雌激素对雏鸡生长性能及骨代谢的影响
第一部分 选用7日龄健康雄性雏鸡160只,试验分组同第一章第一部分,试验期持续4周。结果表明,与对照组相比,21日龄时,HK90组、HK99组、HK03组和HK04组每g肌组织RNA含量显著升高(P<0.01),分别较对照组升高了10.98%、12.38%、7.97%和9.04%,总RNA含量分别较对照组提高了23.71%(P<0.05)、16.05%(P>0.05)、21.46%(P<0.05)和28.88%(P<0.05),而DNA含量及总DNA含量无显著变化;35日龄时,每g肌组织中RNA含量较对照组显著提高(P<0.05),分别较对照组提高9.09%、8.91%、7.81%、8.00%,而总RNA含量有升高趋势,与对照组差异不显著,DNA含量无显著变化,而总DNA含量有升高的趋势。21日龄时,血清尿酸含量较对照组下降了16.50%(P<0.05)、16.16%(P<0.05)、15.02%(P<0.05)和21.48%(P<0.01);35日龄时,较对照组减少了9.07%、19.38%、6.14%和17.79%,与对照组差异不显著(P>0.05)。
第二部分 血清样品来自第一部分各组雏鸡。结果表明,21日龄时,与对照组相比,
The effect of isoflavonic phytoestrogens on the growth performance in young chickens and involved thyroid hormones were studied in this study.1. Effect of Diet Supplemented with Isoflavonic phytoestrogens on the Growth Performance in 21-day-old Chickens120 7-day-old male chickens were randomized into 5 groups: basal diet(Con group),basal diet plus 5mg/kg HK90(HK90 group), basal diet plus 5mg/kg HK99(HK99 group), basal diet plus 5mg/kg HKO3(HKO3 group), basal diet plus 5mg/kg HK04(HK04group). Compared with control,the experiment groups increased chickens body weight gain per day,the conversion rate. Compared with control,the experiment groups'tissue weight gains had the tendency of increase,but there were no significant difference;the weight of experiment groups'muscle increased by 9.88 % (P<0.05 )、 6.89 % (P>0.05)、 7.49% (P=0.08) and 7.49% (P=0.06) respectively;the weight of experiment groups' thigh increased by 15.19%(P<0.05)、 11.79%(P=0.06)、 15.42%(P<0.05) and 16.782. Effect of Diet Supplemented with Isoflavonic phytoestrogens on the Growth Performance and the Metabolism of Bone in ChickensPart 1 160 7-day-old male chickens were randomized into 5 groups as chapter 1 part 1.The results indicated were as follows. When they were 21 days old, compared with control, the content of RNA level in per gramme chickens' muscle were increased significantly(P<0.01),increased by 10.98%,12.38%,7.97% and 9.04% respectively in the four experiment groups,the total RNA increased by 23.71%(P<0.05), 16.05%(P>0.05), 21.46% (P<0.05)and 28.88%(P<0.05),but the content of DNA and the total DNA were no
significant difference.The serum uric acid concentration of experiment groups were decreased by 16.50%(P<0.05),16.16%(P<0.05),15.02%(P<0.05) and 21.48%(P<0.01), significantly. When they were 35 days old, compared with control,the content of RNA level in per gramme chickens' muscle were increased significantly(P<0.05),increased by 9.09%, 8.91%,7.81% and 8.00%,the total RNA had the tendency of increase,the content of DNA were similar with control and the total DNA had the tendency of increase. The serum uric acid concentration of experiment groups were decreased by 9.07%, 19.38%,6.14% and 17.79%,but there were no significant difference with control(P>0.05). Part 2 The serum samples came from part 1. When they were 21 days old, compared with control, inorganic phosphorus level were increased by 65.41%(P<0.01), 35.14%(P<0.05), 68.65%(P<0.01) and 94.59%(P<0.01) ,significantly, HK90 group' serum BGP level were increased by 41.53%(P<0.01),the other groups' serum BGP level had the tendency of increase(P>0.05),the AKP activity had the tendency of increase and the calcium level had no significant difference. When they were 35 days old, compared with control,HK99 group' serum calcium level were decreased by 11.19%(P<0.05),HK04 group' inorganic phosphorus were increased by 9.72%(P<0.05),HK99 and HK03 groups' AKP activity were increased by 136.41%(P<0.01) and 108.28%(P<0.01),the other groups' calcium level had the tendency of decrease and inorganic phosphorus,BGP,AKP had the tendency of increase(P>0.05).3. Effect of Diet Supplemented with Isoflavonic phytoestrogens on the Thyroid HormonesThe serum samples came from chapter 2 part l.T3,T4,FT3,FT4 and T were analyzed by radio immunoassay(RIA).When they were 21 days old,compared with control,the serum T3 level were decreased by 19.34%(P<0.05),23.36%(P<0.01),41.24%(P<0.01) and 39.05% (P<0.01) respectively, HK99 group' serum T4 level were increased by 26.91%(P<0.05), HK04 group' serum free T3 level were increased by 46.43%(P<0.01),the other groups' T4 free T3 and free T4 had the tendency of increase(P>0.05).When they were 35 days old, compared with control HK03 and HK04 groups' serum T3 level were decreased by 30.16%(P<0.01) and 22.22%(P<0.05),HK90 group' serum free T3 level were increased by 62.82%(P<0.01),the other groups' serum T3 had the tendency of decrease and free T3 level had the tendency of increase(P>0.05);compared with control.the experiment groups' T4 level had the tendency of increase(P>0.05).
引文
1.王玢,刘叔义主编.人体及动物生理学(第二版)[M].北京:高等教育出版社,2001:429
2. Hammand Jr J.et al. General principles--metabolities and growth Hammand's farm animals[J]. 1983, 21-24
3.韩正康主编.家畜营养生理学[M].北京:农业出版社,1993:40-51
4.刘培楠.多肽生长因子概论[J].生理科学,1986,6(4):193
5.裴雪涛主编.干细胞生物学[M].北京:科学出版社,2003
6.薛庆善主编.体外培养的原理与技术[M].北京:科学出版社,2001:615
7.马克昌,冯坤,朱太咏等主编。骨生理学[M].郑州:河南医科大学出版社,2000:223-258
8.安胜军,杨学辉,李恩.雌激素与骨质疏松症[J].中国骨质疏松杂志,1999,5(3):77-85
9.鞠传广,马庆军.雌激素对骨重建过程中的影响[J].8(4):372-373
10. Pacifici R.Is there a causaln role of IL-1 in postmenopausal bone loss?[J]Calif Tissu Int, 1992,4:676-685
11.苏佳灿,许硕贵.骨生长因子与骨重建研究进展(综述)[J].临床骨科杂志,2001,4(2):155-157
12.李郑芳.胰岛素样生长因子—1与骨重建(综述)[J].国外医学内分泌学分册,1994,14(4):186-188
13.周尊海,李荣华.白细胞介素—4与骨重建(综述)[J].国外医学内分泌学分册,1996,16(2):92-93
14.柯俐,陈璐璐.整合素与骨重建(综述)[J].中国骨质疏松杂志,1999,5(1):79-84
15.谢启文主编.现代神经内分泌学[M].上海:上海医科大学出版社,1999:26-58
16.艾晓杰,韩正康.禽类生长的神经内分泌调控(综述)[J]上海交通大学学报(农业科学版),2001,19(2)154—159
17.张在香.脱碘酶(综述)[J].卫生研究,1998,27(5):358-360
18. Kuhn E R,Berghman L R,Moons L,etal.Hypothalamicand peropheral control of thyroid function during the life cycle of the chicken P J Sharp Avian Endocrinnoogy Almoodsbury Bristo Burgess Science Press. 1993,29-46
19.张镜如主编.生理学(第四版)[M].北京:人民卫生出版社,2000:382-385
20.杨钢主编.内分泌生理与病理生理学[M].天津:天津科学技术出版社,1996:210-423
21.韩正康,王国杰.异黄酮植物雌激素:反刍动物营养生物学发展与应用前景[J].动物营养学报,1999,1(11)Supple:65-68
22.王国杰.韩正康.植物异黄酮研究新进展[J].天然产物研究与开发,1994,6(3):81-83
23. Wolf A S.Phytoestrogen[J].J Med Assoc Thai. 1998,81 ( 1 )Supple :49-53
24.刘颖,滕燕平,张玉梅.膳食异黄酮的研究进展(综述)[J].解放军预防医学杂志,2000,18(5):384-386
25.毛峻琴,宓鹤鸣.大豆异黄酮的研究进展[J].中草药,2000,31(1):61-64
26.崔洪斌主编.大豆生物活性物质的开发与应用[M].北京:中国轻工业出版社,2001:144
27.孟祥颖,鲁歧,赵英等.葛藤与葛根中异黄酮类成分的比较[J].植物资源与环境,1996,4:26-28
28.孙玲,张孝祺,陈俊秋等.大豆异黄酮的研究和应用[J].中国粮油学报,2002,17(2):41-44
29. Eldridge A C,Kwolek W ESoybean isoflavones:effect of environment and variety on composition[J] Agric Food Chem. 1983,31(2):394-396
30.孙君明,丁安林.大豆异黄酮的含量及影响因素的评价[J].中国粮油学报,1998,13(2):10—14
31.潘志芬,潘开文.大豆及大豆制品中异黄酮的研究现状[J].四川农业大学学报,1999,19(2):217-223
32.顾晓华,杨爱华,宗志敏等.大豆异黄酮[J].精细与专用化学品,2001,6:11-12
33. Adrian A F. et al.Quantitation of phytoestrogens in legumes by HPLC[J]. Agric Food Chem. 1994,42:1905-1913
34.张响英,王根林,唐现文.异黄酮植物雌激素在动物中的作用[J].饲料研究,2000,11:12-14
35. King R A,Bursill D B.Plasma and urinary kinetics of the isoflavones daidzein and genistein after a single soy meal in human[J].Am J Nutrition. 1998,60:867-872
36. Lampe J W, Martini M C,Kruzer M S,et al.Urinary lignan and isoflavonoid excreation in premenopausal women consuming flaxseed powder[J]. Am J Nutr. 1994,60:122-128
37. Peach K, Webb P, Kuiper G W, et al.Differential ligand activation of estrogen receptors Eralpha and Erbeta at AP 1 sites[J].Science. 1997,277:1508-1510
38.张玉梅.大豆异黄酮的生物利用度[J].国外医学卫生学分册,2001,28(2)104-107
39. Xu X, Harris K S, Wang H J, et al. Bioavailability of soybean isoflavones depends upon gut micoflora in women[J].Nutrition. 1995,125:2307-2315
40. Xu X, Wang H J,Murphy P A,et al. Daidzein is more bioavailability soybean isoflavone than is genistein in adult women[J].Nutrition.1994,124:825-832
41. Tew B Y, Xu X, Wang H J, et al.A diet high in wheat fiber decreases the bioavailability of soybean isoflavones in a single meal fed to women[J].Nutrition. 1996,126:871-877
42.彭向雷,王蕊,王悦等.大豆异黄酮研究进展[J].中国食品卫生杂志,1998,10(3):38-41
43.周玲,苏黎红,侯金玲.大豆异黄酮研究概况[J].时珍国医国药,2001,12(2):157-158
44.王陶,王辉.植物雌激素及其对人类的作用[J].湖南中医药导报,1999,5(2):14-16
45. Shaw Watanabe.Pharmacokinetics of soybean isoflavones in plasma,urine and frees of men after ingestion of baked soybean powder[J].Nutr. 1998,10:1710-1715
46. Yah Zhang.Urinary disposition of the soybean isoflavones Daidzein and Glycitein differs among humans with moderate fecal isoflavone degradation activity[J]. Nutr. 1999, 129: 957-962
47. A A Frake. Daidzein and Genistein concentrations in human milk after soy consumption[J]. Clinical Chemistry. 1996, 42: 955-964
48. Setchell K D, Brown N M, Lydeking-OIsen E. The clinical importance of the metabolite equol-a clue to the effectiveness of soy and its isoflavones[J]. Nutr. 2002, 132(12): 3577-3584
49. Lundh T. Metabolism of estrogenic isoflavones in domestic animals[J]. Proc Soc Exp Bio Med. 1995, 208(1): 33-39
50. Dickinson J M, Smith G R, Randel R D, et al. In vitro metabolism of formononetin and biochanin A in bovine rumen fluid[J]. Anim Sci. 1988, 66: 1969-1973
51. Braden A, Thain R L, Shutt D A, et al. Comparison of plasma phytoestrogens levels in sheep and cattle after feeding on fresh clover[J]. Aus J Agric Res. 1971, 22: 663-670
52.于文三编译.植物雌激素研究进展[J].国外医学卫生学分册,1999,26(2):84-87
53. Wiseman H. Role of dietary phytoestrogens in the pyotection against cancer and heart disease[J]. Biochem Soc Trans. 1996, 24(3): 795-800
54.陈霞.大豆异黄酮生理功能及其应用前景[J].黑龙江农业科学,2002,6:35-38
55. Herman C, Adlercreutz T. Goldin B R, et al. Soybean phytoestrogen intake and cancer risk[J]. Nutr. 1995, 125: 757S-770S
56. Shutt D A, Cox R I. Steroid and phytoestrogen binding to sheep uterine receptors in vitro[J]. Endocrinology. 1972, 52: 229
57. Zava D T. Estrogenic bioactivity of phytoestrogens in human breast cancer in monolayer culture[J]. Nutr. 1995, 125: 807
58. Mousavi Y. et al. Steroids. 1993, 58: 301-304
59. Peterson T G. et al. Biochemistry Biophysics Research Commun. 1991, 179: 661-667
60.朱俊东.大豆异黄酮抗癌作用的研究进展(综述)[J].国外医学卫生学分册,1998,25(5)257-260
61.郑杰.金雀异黄素[J].国外医学卫生学分册,1998,25(5):260-263
62.周素梅.食物中的植物化学成分及功能性[J].食品与机械,1999,1:13-14
63. Anthony M S. Soybean isoflavones improve cardiovascular risk factors without affecting reproductive system of peripubertal rhesus monkeys[J]. Nutr. 1996, 126: 43-50
64. Yamazaki I. Calcitonin secreting property of ipriflavone in the presence of estrogen[J]. Life Sci. 1986, 38: 1535-1541
65. Samarmoudy F A. Aderverse effects of phytoestrogen effect of berasitsterol treatment on follicular development ovarian structure and uterus in the immature female sheep[J]. Cell Molec Biol. 1980, 26: 255-266
66.张荣庆,程丽仁,张崇理.植物异黄酮Daidzein对雌性大鼠性周期及血浆中LH、FSH水平的影响[J].生理通讯,1995,14(增刊):49
67.韩正康.异黄酮植物雌激素调控动物神经内分泌及生产性能的研究[J].中国农业科技导报,1999,1:61-66
68.张荣庆,程丽仁,沈卫斌等.植物异黄酮Daidzein对去卵巢大鼠血浆GnRH、β-ER、LH和FSH水平的影响.中国生理学会第四界神经内分泌学术会议.青岛生理通讯,1995,14(增刊):48
69. Wang Genlin, Chen Jie, N Parvizi LH. Secretion following single injection and implantation of daidzein in ovariectomized gilts. 2nd Asian Symposium on Animal Biotechnology. 1996: 433-437
70.王根林,陈杰,韩正康等.猪垂体体外表面灌流大豆黄酮对LH分泌的影响[J].生理通讯,1996,15(增刊4):62-63
71.王国杰,韩正康.红三叶草总异黄酮对小公鸡生长及血清睾酮水平的影响[J].动物学研究,1994,15(3):65-69
72.刘燕强,韩正康.异黄酮植物雌激素—大豆黄酮对产蛋鸡生产性能及血液中几种激素水平的影响[J].中国畜牧杂志,1998,34(3):9-10
73.朱建平等.大豆黄酮对樱桃谷肉种鸭生产性能的影响[J].饲料工业,2002.23(1) 34-35
74.王伟群,韩正康,陈伟华等.芒柄花素的促乳腺发育作用及其机理探讨[J].南京农业大学学报,1993,S16:19-24
75.刘德义,周玉传,陆天水等.大豆异黄酮对乳牛产奶量和乳脂率及饲料转化率的影响[J].中国畜牧杂志,2004,40(4):31-32
76. Adams NR. Detection of the effects of phytoestrogens on sheep and cattle[J]. Animal Science. 1995. 73: 1509-1515
77.刘根桃,郑元林,陈伟华等.妊娠后期母猪饲喂大豆黄酮对泌乳性能及初乳中激素水平的影响[J].南京农业大学学报,1999,22(1):69-72
78.张荣庆,韩正康,陈杰等.大豆黄酮对大鼠乳腺发育作用的实验研究[J].动物学报,1995,41(3) 332-338
79.张荣庆,韩正康,陈杰等.大豆黄酮促进妊娠大鼠乳腺发育和泌乳的实验研究[J].动物学报,1995,41(4):414-419
80.杨丽杰.大豆异黄酮在畜牧生产中的应用[J].中国饲料,2004,9:23-25
81.韩正康.异黄酮植物雌激素—大豆黄酮对雄性动物生长及其有关内分泌的研究[J].畜牧与兽医,1999,31(1):1-2
82.郭慧君,韩正康,王国杰.日粮添加大豆黄酮对去势仔猪生长性能及有关内分泌的影响[J].中国畜牧杂志,2002,38(2):17～18.
83.薛竹风.大豆黄酮对肥育猪生产性能和猪体外形的影响[J].兽药与饲料添加剂,2000,5:24
84.郭慧君,韩正康,王国杰.日粮添加大豆黄酮对大鼠生长性能和有关内分泌的影响[J].南京农业大学学报,2001,24(4):59-61
85.王国杰,韩正康,陈伟华.大豆黄酮对大鼠肌肉生长和几种内源激素水平的影响[J].动物学研究,1995,16(1):23-29
86.张荣庆,韩正康.异黄酮植物雌激素IFD对胎儿生长的影响[J].生理通讯,1993,12(增刊):153
87.杨国宇,陈杰,陈伟华等.IFV-D对反刍动物内源性睾酮水平及瘤胃消化代谢的影响.畜禽优质高产生物调控研讨会论文摘要汇编,中国畜牧兽医学会动物生理生化学会,1994,29
88.王伟群,韩正康,陈杰.饲喂红三叶草后湖羊瘤胃液和血液中芒柄花素浓度的变化[J].南京农业大学学报,1991,14(2):97-98
89.王伟群,韩正康.黄酮类化合物对瘤胃中主要消化酶活力的影响.全国动物生理生化第二次学术会议论文摘要汇编,中国畜牧兽医学会动物生理生化学会,1990:26-27
90.韩正康.黄酮类化合物调控畜禽神经内分泌及生产性能的研究,畜禽优质高产生物调控研讨会论文摘要汇编,1994:1-3
91.张汤杰,陈伟华,陈杰.注射大豆黄酮对仔猪淋巴细胞转化率影响的研究[J].江苏农学院学报,1998,19(4):86-88
92.屈健.异黄酮类化合物的生物学功能及其在养殖业中的应用[J].兽药与饲料添加剂,2002,7(4):18-20
93.张荣庆,韩正康,陈杰等.大豆黄酮对母猪免疫功能和血清及初乳中GH、PRL、SS水平的影响[J].动物学报,1995,41(2):201-206
94.高峰,周光宏,韩正康.大豆黄酮对雏公鸡生长性能和机体免疫的影响[J].中国家禽,2000,22(10):8-9
95.吴天华,蒋雨,罗文华等.大豆黄酮在畜禽中的作用机理及应用效果[J].畜禽业,2003,7:28-29
96. Gray T K. 13-β esdtradiol acts directly on the osteoblast cell line UMR 160[J]. Proc Natl Acad Sci USA. 1987, 84: 6267-6271
97. Komm B S. Estrogrn binding receptor mRNA and biologic response in osteoblast-like osteosarcom a cell[J]. Science. 1988, 241: 81-84
98. Oursle M J, Osdoby P, Pyfferoen J, et al. Avian osteoclast as estrogen target cells[J]. Proc Natl Acad Sci USA. 1991, 88: 6613-6617
99. Onoe Y, Miyaura C, Ohta H, et al. Expression of estrogen receptor-β in rat bone[J]. Endocrinol. 1997, 138: 4509-4512
100.刘燕强.碱性磷酸酶及其在兽医诊断中的价值[J].内蒙古畜牧科学,1994,2:23-26
101.吴文苑.骨型碱性磷酸酶的检测方法和临床应用[J].国外医学临床生物化学与检验学分册,1999,20(2):64-65
102.陈民利,应华忠,史国荣等.金葡液对小鼠血清碱性磷酸酶活力的影响[J].上海实验动物科学,2000,20(3):157-158
103.曹守凤,李海浪.骨钙素与儿童生长发育[J].国外医学妇幼保健分册,2004,15(5):259-261
104.杨建辉,刘淼,黄茹等.雌激素对离体成骨细胞成骨能力的影响[J].2004,25(1):48-50
105. Anderson J, Ohue T, Daenzer M, et al. Effects of estrogens, phytoestrogens and antiestrogens in ROS and ROS SMER cell[J]. Bone. 1998, 22(2): s2
106. Yamaguchi M and Ying Huagao. Inhibitory effect of genistein on bone resorption in tissue culture[J]. Biochem Pharm. 1998, 55(1): 71-76
107. Yamaguchi M and Ying Huagao. Anabolic effect of genistein and genistein on bone metabolism in the femoral-metaphyseal tissues of elderly rats: the genistein effect is enhanced by zinc[J]. Mol Cell Biochem. 1998, 178(1-2): 377-382
108. Daito Nakajima, Chang-Sun Kim, Tae-Woong Oh, et al. Suppressive effects of genistein dosage and resistance exercise on bone loss in ovariectomiaed rats[J]. Physiol Anthropol. 2001, 20(5): 285-291
109.李斌斌,于世凤,庞淑珍.大豆异黄酮对骨代谢影响的组织学观察[J].现代口腔医学杂志,2004,18(5):385-388
110. Fanti O. The phytoestrogen genistein reduces bone loss in short-term ovariectomized rats[J]. Osteoporos Int. 1998, 8(3): 274-281
111. Gao Y H, Yamaguchi. Inhibitory effect of genistein on osteoclast-like cell formation in mouse marrow cultures[J]. Biochem Pharm. 1999, 58: 767-772
112. Giossi M, Caruso P. Inhibition of parathyroid hormones-stimulated resorption in cultured fetal rat long bones by the main metabolites of ipriflavone[J]. Calcif Tissue Int. 1996, 58: 419-422
113. Civitelli R. In vitro and in vivo effects of ipriflavone on bone formation and bone biomechanics. Calcif Tissue Int. 1997, 61: 12
114.李斌斌,于世凤,庞淑珍.染料木黄酮对大鼠颅盖骨骨吸收活性的作用[J].牙体牙髓牙周病学杂志,2003,13(12):672-674
115.马慧萍.淫羊藿防治骨质疏松症的研究进展(综述)[J].兰州医学院学报,2001,27(4):69-71
116.陈保红.大豆异黄酮作用机制及在动物生产中的应用[J].动物科学与动物医学,2003,20(10):53-55
117. Wang G J, Ke Y Y, Han Z K. Anti-aging effect of daidzein in aged mice. May 28-June 2, 1999, Tampere, Finland, (Abstract Book): P4.22
118.闫祥华.大豆异黄酮的抗癌作用机制研究进展[J].生理科学进展,1997,28(4):326-364
119. Cai Q. Effect of dietary genistein on antioxidation enzyme activities in SENCAR mece[J]. Nutr Cancer. 1996, 25: 1-7
120.郑高利.大豆异黄酮的抗氧化作用[J].浙江医科大学学报,1997,26(5):196-199
121.朱新建,韩正康,王国杰.日粮中添加大豆黄酮对蛋鸡抗氧化能力的影响[J].畜牧与兽医,2004,36(9):6-7
122.刘文等.大豆黄酮对衰老小鼠脑组织抗氧化能力的影响[J].徐州师范大学学报,2004,22(4):41-44
123.叶艳彬,卓淑雨,苏宜香等.大豆异黄酮类对去卵巢大鼠内分泌和抗氧化系统的影响[J].中国老年学杂志,2005,25(1):75-77
124. Tikkenen M J,Allercreutz H.Dieetary soy derived isoflavonic phytoestrogens,could they have a role in coronary heart disease prevention[J].Biochem Pharmacol,2000,60(1): 1-5
125. Scheiber M D,Liu J H,Subbiah M T, etal.Dietary inclusion of whole soy food results in significant reductions in clinical risk factors for obstetrics and cardiovascular disease in normal postmenopausal women[J].Menopause.2001,8(5):384-392
126. Jenkins D J,Kendall C W, etal.Effects of soy protein foods on low-density lipoprotein oxidation and ex vivo sex hermone receptor activity-a controlled crossover trial [J] Metabo lism .2000,49(4):537-543
127.闫祥华,顾景范,孙存普.大豆异黄酮抗脂质过氧化作用及其机制初探[J].解放军预防医学杂志,2000,18(1):14-17.
128.吴定,江汉湖.发酵大豆制品中异黄酮形成及其功能[J].中国调味品,2001,6:3-6
129. Arora A,Byrem T M, Nair M G, et al.Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids[J].Biochem Biphys.2000,373:102-109
130. Arora A,Nair M G, Strasburg G M.Antioxidant activities of isoflavones and their metabolites in a liposomai system[J].Biochem Biphys. 1998,356:133-141
131. Kameoka S,Leavitt P, Chang C,et al.Expression of antioxidant proteins inhuman Caco-2 cells treated with dietary flavonoids[J].Cancer Lett. 1999,146:161-167
132. Chan W H,Yu J S.Inhibition of irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermal carcinoma A431 cells by genistein[J].Cell Biochem.2000,78:73-84
133.刘丽,金宏.大豆异黄酮抗氧化作用的研究进展[J].中华放射医学与防护杂志,2003,23(2):132-134
134. Messina M J,Persky V, Setchell K D R,et al.Soy intake and cancer risk:a review of the in vitro and in vivo data[J].Nutr Cancer. 1994,21:113
135. Pollard M,Luckert P H.Influence of isoflavones in soy protein isolates on development of induced prostate-related cancers in L-W rats[J]. Nutr Cancer. 1997, 28(1): 41
136. Zeming J, Macdonald R S. Soy isoflavones latency of spontaneous mammary tumors in mice[J]. Nutr. 2002, 132: 3186-3190
137. Barnes R, Petrson T G, Grubbs C. In diet and Cancer: Markers, prevention and treatment. New York Plenum Press. 1994, 135
138. Sharma O P, Grubbs C, Setchell K D, et al. Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats[J]. Steroid Biochem Mol Biol. 1992, 43(6): 557-564
139.杨峥嵘,程天民,何飞.genistein对人乳腺癌抑瘤效应及造血辐射防护的实验研究[J].中华肿瘤杂志,2002,24(6):548
140. Verma S P, Goldin B R, Lin P S, The inhibition of the estrogenic effects of pesticides and environmental chemicals by curcumin and isoflavonoids[J]. Environmental Health Perspect. 1998, 106(12): 807-812
141. Nakagava H, Yamamoto D, Kiyozuka Y, etal. Effects of genistein and synergistic action in combination with eicosapentaenoic acid on the growth of breast cancer cell lines[J]. Cancer Res Clin Oncol. 2000, 126(8): 448-454
142. Constantinou A I, Krygier A E, Metha R R. Genistein includes maturation of cultured human breast cancer cells and prevents tumor growth in nude mice[J]. Clin Nutr. 1998, 68(6 Supple): 1426S-1430S
143. Allred C D, Allred K F, Ju Y H, etal. Soy diets containing varying amounts of genistein stimulate growth of estrogen-dependent (MCF-7) tumors in a dose-dependent manner[J]. Cancer Res. 2001, 61(13): 5045-5050
144. Ju Y H, Allred C D, Allred K F, etal. Physiological concentrations of dietary genistein dose-dependently stimulate growth of estrogen-dependent human breast cancer (MCF-7) tumors imolanted in athymic nude mice[J]. Nutr. 2001, 131(11): 2957-2962
145.李咏梅,厉曙光.大豆异黄酮抗癌作用的研究进展[J].同济大学学报,2002,23(4):351-353
146.谢明杰等.大豆异黄酮生理功能的研究进展[J].食品与发酵工业,2004,30(5):94-98
147.杨镇洲.大豆异黄酮的抗癌效应研究进展[J].国外医学肿瘤学分册,2001,28(2):107-110
148.王晓明,付国财,王震武.大豆甙元取代饲料中抗生素添加剂饲喂仔猪试验[J].湖北农业科学,2002,1:69-70
149.黄进,罗琼,李晓莉等.大豆异黄酮的降血糖活性研究[J].食品科学,2004,25(1):1-5
150.郭舜民,郭尧惠.天然药降血糖成分的研究进展[J].海峡药学,2000,12(1):1-5
151.王林山,陈月英.大豆异黄酮的研究进展[J].食品研究与开发,2004,25(5):14-17
152. Michael Nain, Benjamin Gesterner, Shmuel Zilkah, etal. Soybean isoflavones characterization, determination and antifungal activity[J]. Agri Food Chem. 1974, 22(5): 806-810
153.谢明杰,陆敏,邹翠霞等.大豆异黄酮的抑菌作用[J].大豆科学,2004,23(2):101-105
154.刘刚,刘东,张晓喻.大豆异黄酮对皮肤保水活性的研究[J].西南民族大学学报(自然科学版),2003,29(4):429-430
155.朱建林,杨桂莲,黄忆明.大豆异黄酮对去卵巢小鼠认知功能的影响[J].中南大学学报(医学版),2004,29(1):81-83
156. Weber K S, Setchell K D, Stocco D M, etal. Dietary soy phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5 alpha reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats[J]. Endorcrinal, 2001, 170(3): 591-599
157. Lund T D, Rhees R W, Setchell K D, etal. Altered sexually dimorphic nucleus of the preoptic area (SDN-POA) volume in adult Long Evans rats by dietary soy phytoestrogens[J]. Brain Res. 2001, 914(1-2): 92-99
158. Kwon S M, Kim S I, Chun D C, etal. Development of rat protatitis model by oral administration of isoflavone and its characteristics[J]. Yonsei Med. 2001, 42(4): 395-404
159.任国峰,黄忆明.大豆异黄酮对大鼠前列腺增生的抑制作用[J].湖南医科大学学报,2003,28(4):243-246
160.朱心强.外来雌激素及其对雄性生殖系统发育的影响[J].国外医学卫生学分册,1995,22(4):212-216
161.伍喜林,郭春华.植物性类激素物质的营养作用研究与开发前景[J].畜禽业,2002,9:38-39
162.朱泽远,周维仁,乐国伟.异黄酮植物雌激素对动物生长的影响及其作用机制[J].兽药与饲料添加剂,2003,5:25-27
163.赵志辉,徐银学,陆天水等.大豆黄酮对2周龄仔猪增重及肝脏GH受体的影响[J].上海交通大学学报(农业科学版),2003,21(1):40-43
164.耿志荣.大豆黄酮和染料木素的RP-HPLC方法的建立及其在禽类体内蛋内含量的研究.南京农业大学硕士论文,2003
165. Han Z K, Wang G J. Studies of phytoestrogens(Daidzein) affecting on growth and sex hormone levels in broiler. ⅦWorld conference on animal production, Edmonton Cananda, 1993: 152-153
166.王国杰,韩正康,陈杰等.大豆黄酮对肉鸡生长的影响及其作用机制的研究[J].广东畜牧兽医科技,1994,19(3):4-6
167.李斌斌,于世凤.Genistein对破骨细胞分化和骨吸收功能的影响及其机制探讨[J].中国骨质疏松杂志,2004,10(3):272-276
168.张宏昱,张石革.依普黄酮的研究进展与临床应用[J].中国医院用药评价与分析,2002,2(6):363-364
169.柯叶艳,王国杰,韩正康.日粮中添加大豆黄酮对鹌鹑生产性能和血液中激素水平的影响[J].畜牧 兽医学报,2002,33(3):243-246
170. Cogburn L A, Nien-chen Mao, etal. Interaction between somatotropic and thyrotropic axes in regulation of growth and development of broiler chickens[J]. European Poultry Science. 1995, E20093: 18-21
171.赵茹茜,周玉传,徐银学等.大豆黄酮对高邮鸭增重及血清某些激素水平和垂体mRNA表达的影响[J].农业生物技术学报,2002,10(2):176-179
172.杨黎娟.雌激素抗骨质疏松的研究进展(综述)[J].国外医学内科学分册,2004,31(2):84-87
173.周振雷,侯加法.雌激素对骨代谢的调节作用(综述)[J].中国临床康复,2004,8(14):2714-2715
174.马学会,武现军,倪耀娣等.大豆黄酮对产蛋鸡蛋壳品质和骨骼代谢的影响[J].饲料工业,2004,25(7):32-34
175.王媛,常红,黄国伟等.大豆异黄酮对体外原代大鼠成骨细胞增殖、分化的影响[J].天津医科大学学报,2004,10(1):53-55
176.王建华,佟晓旭,陈永春等.大豆甙元对大鼠成骨细胞碱性磷酸酶活性的影响[J].中药药理与临床,2003,19(4):8-9
2. Hammand Jr J.et al. General principles--metabolities and growth Hammand's farm animals[J]. 1983, 21-24
3.韩正康主编.家畜营养生理学[M].北京:农业出版社,1993:40-51
4.刘培楠.多肽生长因子概论[J].生理科学,1986,6(4):193
5.裴雪涛主编.干细胞生物学[M].北京:科学出版社,2003
6.薛庆善主编.体外培养的原理与技术[M].北京:科学出版社,2001:615
7.马克昌,冯坤,朱太咏等主编。骨生理学[M].郑州:河南医科大学出版社,2000:223-258
8.安胜军,杨学辉,李恩.雌激素与骨质疏松症[J].中国骨质疏松杂志,1999,5(3):77-85
9.鞠传广,马庆军.雌激素对骨重建过程中的影响[J].8(4):372-373
10. Pacifici R.Is there a causaln role of IL-1 in postmenopausal bone loss?[J]Calif Tissu Int, 1992,4:676-685
11.苏佳灿,许硕贵.骨生长因子与骨重建研究进展(综述)[J].临床骨科杂志,2001,4(2):155-157
12.李郑芳.胰岛素样生长因子—1与骨重建(综述)[J].国外医学内分泌学分册,1994,14(4):186-188
13.周尊海,李荣华.白细胞介素—4与骨重建(综述)[J].国外医学内分泌学分册,1996,16(2):92-93
14.柯俐,陈璐璐.整合素与骨重建(综述)[J].中国骨质疏松杂志,1999,5(1):79-84
15.谢启文主编.现代神经内分泌学[M].上海:上海医科大学出版社,1999:26-58
16.艾晓杰,韩正康.禽类生长的神经内分泌调控(综述)[J]上海交通大学学报(农业科学版),2001,19(2)154—159
17.张在香.脱碘酶(综述)[J].卫生研究,1998,27(5):358-360
18. Kuhn E R,Berghman L R,Moons L,etal.Hypothalamicand peropheral control of thyroid function during the life cycle of the chicken P J Sharp Avian Endocrinnoogy Almoodsbury Bristo Burgess Science Press. 1993,29-46
19.张镜如主编.生理学(第四版)[M].北京:人民卫生出版社,2000:382-385
20.杨钢主编.内分泌生理与病理生理学[M].天津:天津科学技术出版社,1996:210-423
21.韩正康,王国杰.异黄酮植物雌激素:反刍动物营养生物学发展与应用前景[J].动物营养学报,1999,1(11)Supple:65-68
22.王国杰.韩正康.植物异黄酮研究新进展[J].天然产物研究与开发,1994,6(3):81-83
23. Wolf A S.Phytoestrogen[J].J Med Assoc Thai. 1998,81 ( 1 )Supple :49-53
24.刘颖,滕燕平,张玉梅.膳食异黄酮的研究进展(综述)[J].解放军预防医学杂志,2000,18(5):384-386
25.毛峻琴,宓鹤鸣.大豆异黄酮的研究进展[J].中草药,2000,31(1):61-64
26.崔洪斌主编.大豆生物活性物质的开发与应用[M].北京:中国轻工业出版社,2001:144
27.孟祥颖,鲁歧,赵英等.葛藤与葛根中异黄酮类成分的比较[J].植物资源与环境,1996,4:26-28
28.孙玲,张孝祺,陈俊秋等.大豆异黄酮的研究和应用[J].中国粮油学报,2002,17(2):41-44
29. Eldridge A C,Kwolek W ESoybean isoflavones:effect of environment and variety on composition[J] Agric Food Chem. 1983,31(2):394-396
30.孙君明,丁安林.大豆异黄酮的含量及影响因素的评价[J].中国粮油学报,1998,13(2):10—14
31.潘志芬,潘开文.大豆及大豆制品中异黄酮的研究现状[J].四川农业大学学报,1999,19(2):217-223
32.顾晓华,杨爱华,宗志敏等.大豆异黄酮[J].精细与专用化学品,2001,6:11-12
33. Adrian A F. et al.Quantitation of phytoestrogens in legumes by HPLC[J]. Agric Food Chem. 1994,42:1905-1913
34.张响英,王根林,唐现文.异黄酮植物雌激素在动物中的作用[J].饲料研究,2000,11:12-14
35. King R A,Bursill D B.Plasma and urinary kinetics of the isoflavones daidzein and genistein after a single soy meal in human[J].Am J Nutrition. 1998,60:867-872
36. Lampe J W, Martini M C,Kruzer M S,et al.Urinary lignan and isoflavonoid excreation in premenopausal women consuming flaxseed powder[J]. Am J Nutr. 1994,60:122-128
37. Peach K, Webb P, Kuiper G W, et al.Differential ligand activation of estrogen receptors Eralpha and Erbeta at AP 1 sites[J].Science. 1997,277:1508-1510
38.张玉梅.大豆异黄酮的生物利用度[J].国外医学卫生学分册,2001,28(2)104-107
39. Xu X, Harris K S, Wang H J, et al. Bioavailability of soybean isoflavones depends upon gut micoflora in women[J].Nutrition. 1995,125:2307-2315
40. Xu X, Wang H J,Murphy P A,et al. Daidzein is more bioavailability soybean isoflavone than is genistein in adult women[J].Nutrition.1994,124:825-832
41. Tew B Y, Xu X, Wang H J, et al.A diet high in wheat fiber decreases the bioavailability of soybean isoflavones in a single meal fed to women[J].Nutrition. 1996,126:871-877
42.彭向雷,王蕊,王悦等.大豆异黄酮研究进展[J].中国食品卫生杂志,1998,10(3):38-41
43.周玲,苏黎红,侯金玲.大豆异黄酮研究概况[J].时珍国医国药,2001,12(2):157-158
44.王陶,王辉.植物雌激素及其对人类的作用[J].湖南中医药导报,1999,5(2):14-16
45. Shaw Watanabe.Pharmacokinetics of soybean isoflavones in plasma,urine and frees of men after ingestion of baked soybean powder[J].Nutr. 1998,10:1710-1715
46. Yah Zhang.Urinary disposition of the soybean isoflavones Daidzein and Glycitein differs among humans with moderate fecal isoflavone degradation activity[J]. Nutr. 1999, 129: 957-962
47. A A Frake. Daidzein and Genistein concentrations in human milk after soy consumption[J]. Clinical Chemistry. 1996, 42: 955-964
48. Setchell K D, Brown N M, Lydeking-OIsen E. The clinical importance of the metabolite equol-a clue to the effectiveness of soy and its isoflavones[J]. Nutr. 2002, 132(12): 3577-3584
49. Lundh T. Metabolism of estrogenic isoflavones in domestic animals[J]. Proc Soc Exp Bio Med. 1995, 208(1): 33-39
50. Dickinson J M, Smith G R, Randel R D, et al. In vitro metabolism of formononetin and biochanin A in bovine rumen fluid[J]. Anim Sci. 1988, 66: 1969-1973
51. Braden A, Thain R L, Shutt D A, et al. Comparison of plasma phytoestrogens levels in sheep and cattle after feeding on fresh clover[J]. Aus J Agric Res. 1971, 22: 663-670
52.于文三编译.植物雌激素研究进展[J].国外医学卫生学分册,1999,26(2):84-87
53. Wiseman H. Role of dietary phytoestrogens in the pyotection against cancer and heart disease[J]. Biochem Soc Trans. 1996, 24(3): 795-800
54.陈霞.大豆异黄酮生理功能及其应用前景[J].黑龙江农业科学,2002,6:35-38
55. Herman C, Adlercreutz T. Goldin B R, et al. Soybean phytoestrogen intake and cancer risk[J]. Nutr. 1995, 125: 757S-770S
56. Shutt D A, Cox R I. Steroid and phytoestrogen binding to sheep uterine receptors in vitro[J]. Endocrinology. 1972, 52: 229
57. Zava D T. Estrogenic bioactivity of phytoestrogens in human breast cancer in monolayer culture[J]. Nutr. 1995, 125: 807
58. Mousavi Y. et al. Steroids. 1993, 58: 301-304
59. Peterson T G. et al. Biochemistry Biophysics Research Commun. 1991, 179: 661-667
60.朱俊东.大豆异黄酮抗癌作用的研究进展(综述)[J].国外医学卫生学分册,1998,25(5)257-260
61.郑杰.金雀异黄素[J].国外医学卫生学分册,1998,25(5):260-263
62.周素梅.食物中的植物化学成分及功能性[J].食品与机械,1999,1:13-14
63. Anthony M S. Soybean isoflavones improve cardiovascular risk factors without affecting reproductive system of peripubertal rhesus monkeys[J]. Nutr. 1996, 126: 43-50
64. Yamazaki I. Calcitonin secreting property of ipriflavone in the presence of estrogen[J]. Life Sci. 1986, 38: 1535-1541
65. Samarmoudy F A. Aderverse effects of phytoestrogen effect of berasitsterol treatment on follicular development ovarian structure and uterus in the immature female sheep[J]. Cell Molec Biol. 1980, 26: 255-266
66.张荣庆,程丽仁,张崇理.植物异黄酮Daidzein对雌性大鼠性周期及血浆中LH、FSH水平的影响[J].生理通讯,1995,14(增刊):49
67.韩正康.异黄酮植物雌激素调控动物神经内分泌及生产性能的研究[J].中国农业科技导报,1999,1:61-66
68.张荣庆,程丽仁,沈卫斌等.植物异黄酮Daidzein对去卵巢大鼠血浆GnRH、β-ER、LH和FSH水平的影响.中国生理学会第四界神经内分泌学术会议.青岛生理通讯,1995,14(增刊):48
69. Wang Genlin, Chen Jie, N Parvizi LH. Secretion following single injection and implantation of daidzein in ovariectomized gilts. 2nd Asian Symposium on Animal Biotechnology. 1996: 433-437
70.王根林,陈杰,韩正康等.猪垂体体外表面灌流大豆黄酮对LH分泌的影响[J].生理通讯,1996,15(增刊4):62-63
71.王国杰,韩正康.红三叶草总异黄酮对小公鸡生长及血清睾酮水平的影响[J].动物学研究,1994,15(3):65-69
72.刘燕强,韩正康.异黄酮植物雌激素—大豆黄酮对产蛋鸡生产性能及血液中几种激素水平的影响[J].中国畜牧杂志,1998,34(3):9-10
73.朱建平等.大豆黄酮对樱桃谷肉种鸭生产性能的影响[J].饲料工业,2002.23(1) 34-35
74.王伟群,韩正康,陈伟华等.芒柄花素的促乳腺发育作用及其机理探讨[J].南京农业大学学报,1993,S16:19-24
75.刘德义,周玉传,陆天水等.大豆异黄酮对乳牛产奶量和乳脂率及饲料转化率的影响[J].中国畜牧杂志,2004,40(4):31-32
76. Adams NR. Detection of the effects of phytoestrogens on sheep and cattle[J]. Animal Science. 1995. 73: 1509-1515
77.刘根桃,郑元林,陈伟华等.妊娠后期母猪饲喂大豆黄酮对泌乳性能及初乳中激素水平的影响[J].南京农业大学学报,1999,22(1):69-72
78.张荣庆,韩正康,陈杰等.大豆黄酮对大鼠乳腺发育作用的实验研究[J].动物学报,1995,41(3) 332-338
79.张荣庆,韩正康,陈杰等.大豆黄酮促进妊娠大鼠乳腺发育和泌乳的实验研究[J].动物学报,1995,41(4):414-419
80.杨丽杰.大豆异黄酮在畜牧生产中的应用[J].中国饲料,2004,9:23-25
81.韩正康.异黄酮植物雌激素—大豆黄酮对雄性动物生长及其有关内分泌的研究[J].畜牧与兽医,1999,31(1):1-2
82.郭慧君,韩正康,王国杰.日粮添加大豆黄酮对去势仔猪生长性能及有关内分泌的影响[J].中国畜牧杂志,2002,38(2):17～18.
83.薛竹风.大豆黄酮对肥育猪生产性能和猪体外形的影响[J].兽药与饲料添加剂,2000,5:24
84.郭慧君,韩正康,王国杰.日粮添加大豆黄酮对大鼠生长性能和有关内分泌的影响[J].南京农业大学学报,2001,24(4):59-61
85.王国杰,韩正康,陈伟华.大豆黄酮对大鼠肌肉生长和几种内源激素水平的影响[J].动物学研究,1995,16(1):23-29
86.张荣庆,韩正康.异黄酮植物雌激素IFD对胎儿生长的影响[J].生理通讯,1993,12(增刊):153
87.杨国宇,陈杰,陈伟华等.IFV-D对反刍动物内源性睾酮水平及瘤胃消化代谢的影响.畜禽优质高产生物调控研讨会论文摘要汇编,中国畜牧兽医学会动物生理生化学会,1994,29
88.王伟群,韩正康,陈杰.饲喂红三叶草后湖羊瘤胃液和血液中芒柄花素浓度的变化[J].南京农业大学学报,1991,14(2):97-98
89.王伟群,韩正康.黄酮类化合物对瘤胃中主要消化酶活力的影响.全国动物生理生化第二次学术会议论文摘要汇编,中国畜牧兽医学会动物生理生化学会,1990:26-27
90.韩正康.黄酮类化合物调控畜禽神经内分泌及生产性能的研究,畜禽优质高产生物调控研讨会论文摘要汇编,1994:1-3
91.张汤杰,陈伟华,陈杰.注射大豆黄酮对仔猪淋巴细胞转化率影响的研究[J].江苏农学院学报,1998,19(4):86-88
92.屈健.异黄酮类化合物的生物学功能及其在养殖业中的应用[J].兽药与饲料添加剂,2002,7(4):18-20
93.张荣庆,韩正康,陈杰等.大豆黄酮对母猪免疫功能和血清及初乳中GH、PRL、SS水平的影响[J].动物学报,1995,41(2):201-206
94.高峰,周光宏,韩正康.大豆黄酮对雏公鸡生长性能和机体免疫的影响[J].中国家禽,2000,22(10):8-9
95.吴天华,蒋雨,罗文华等.大豆黄酮在畜禽中的作用机理及应用效果[J].畜禽业,2003,7:28-29
96. Gray T K. 13-β esdtradiol acts directly on the osteoblast cell line UMR 160[J]. Proc Natl Acad Sci USA. 1987, 84: 6267-6271
97. Komm B S. Estrogrn binding receptor mRNA and biologic response in osteoblast-like osteosarcom a cell[J]. Science. 1988, 241: 81-84
98. Oursle M J, Osdoby P, Pyfferoen J, et al. Avian osteoclast as estrogen target cells[J]. Proc Natl Acad Sci USA. 1991, 88: 6613-6617
99. Onoe Y, Miyaura C, Ohta H, et al. Expression of estrogen receptor-β in rat bone[J]. Endocrinol. 1997, 138: 4509-4512
100.刘燕强.碱性磷酸酶及其在兽医诊断中的价值[J].内蒙古畜牧科学,1994,2:23-26
101.吴文苑.骨型碱性磷酸酶的检测方法和临床应用[J].国外医学临床生物化学与检验学分册,1999,20(2):64-65
102.陈民利,应华忠,史国荣等.金葡液对小鼠血清碱性磷酸酶活力的影响[J].上海实验动物科学,2000,20(3):157-158
103.曹守凤,李海浪.骨钙素与儿童生长发育[J].国外医学妇幼保健分册,2004,15(5):259-261
104.杨建辉,刘淼,黄茹等.雌激素对离体成骨细胞成骨能力的影响[J].2004,25(1):48-50
105. Anderson J, Ohue T, Daenzer M, et al. Effects of estrogens, phytoestrogens and antiestrogens in ROS and ROS SMER cell[J]. Bone. 1998, 22(2): s2
106. Yamaguchi M and Ying Huagao. Inhibitory effect of genistein on bone resorption in tissue culture[J]. Biochem Pharm. 1998, 55(1): 71-76
107. Yamaguchi M and Ying Huagao. Anabolic effect of genistein and genistein on bone metabolism in the femoral-metaphyseal tissues of elderly rats: the genistein effect is enhanced by zinc[J]. Mol Cell Biochem. 1998, 178(1-2): 377-382
108. Daito Nakajima, Chang-Sun Kim, Tae-Woong Oh, et al. Suppressive effects of genistein dosage and resistance exercise on bone loss in ovariectomiaed rats[J]. Physiol Anthropol. 2001, 20(5): 285-291
109.李斌斌,于世凤,庞淑珍.大豆异黄酮对骨代谢影响的组织学观察[J].现代口腔医学杂志,2004,18(5):385-388
110. Fanti O. The phytoestrogen genistein reduces bone loss in short-term ovariectomized rats[J]. Osteoporos Int. 1998, 8(3): 274-281
111. Gao Y H, Yamaguchi. Inhibitory effect of genistein on osteoclast-like cell formation in mouse marrow cultures[J]. Biochem Pharm. 1999, 58: 767-772
112. Giossi M, Caruso P. Inhibition of parathyroid hormones-stimulated resorption in cultured fetal rat long bones by the main metabolites of ipriflavone[J]. Calcif Tissue Int. 1996, 58: 419-422
113. Civitelli R. In vitro and in vivo effects of ipriflavone on bone formation and bone biomechanics. Calcif Tissue Int. 1997, 61: 12
114.李斌斌,于世凤,庞淑珍.染料木黄酮对大鼠颅盖骨骨吸收活性的作用[J].牙体牙髓牙周病学杂志,2003,13(12):672-674
115.马慧萍.淫羊藿防治骨质疏松症的研究进展(综述)[J].兰州医学院学报,2001,27(4):69-71
116.陈保红.大豆异黄酮作用机制及在动物生产中的应用[J].动物科学与动物医学,2003,20(10):53-55
117. Wang G J, Ke Y Y, Han Z K. Anti-aging effect of daidzein in aged mice. May 28-June 2, 1999, Tampere, Finland, (Abstract Book): P4.22
118.闫祥华.大豆异黄酮的抗癌作用机制研究进展[J].生理科学进展,1997,28(4):326-364
119. Cai Q. Effect of dietary genistein on antioxidation enzyme activities in SENCAR mece[J]. Nutr Cancer. 1996, 25: 1-7
120.郑高利.大豆异黄酮的抗氧化作用[J].浙江医科大学学报,1997,26(5):196-199
121.朱新建,韩正康,王国杰.日粮中添加大豆黄酮对蛋鸡抗氧化能力的影响[J].畜牧与兽医,2004,36(9):6-7
122.刘文等.大豆黄酮对衰老小鼠脑组织抗氧化能力的影响[J].徐州师范大学学报,2004,22(4):41-44
123.叶艳彬,卓淑雨,苏宜香等.大豆异黄酮类对去卵巢大鼠内分泌和抗氧化系统的影响[J].中国老年学杂志,2005,25(1):75-77
124. Tikkenen M J,Allercreutz H.Dieetary soy derived isoflavonic phytoestrogens,could they have a role in coronary heart disease prevention[J].Biochem Pharmacol,2000,60(1): 1-5
125. Scheiber M D,Liu J H,Subbiah M T, etal.Dietary inclusion of whole soy food results in significant reductions in clinical risk factors for obstetrics and cardiovascular disease in normal postmenopausal women[J].Menopause.2001,8(5):384-392
126. Jenkins D J,Kendall C W, etal.Effects of soy protein foods on low-density lipoprotein oxidation and ex vivo sex hermone receptor activity-a controlled crossover trial [J] Metabo lism .2000,49(4):537-543
127.闫祥华,顾景范,孙存普.大豆异黄酮抗脂质过氧化作用及其机制初探[J].解放军预防医学杂志,2000,18(1):14-17.
128.吴定,江汉湖.发酵大豆制品中异黄酮形成及其功能[J].中国调味品,2001,6:3-6
129. Arora A,Byrem T M, Nair M G, et al.Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids[J].Biochem Biphys.2000,373:102-109
130. Arora A,Nair M G, Strasburg G M.Antioxidant activities of isoflavones and their metabolites in a liposomai system[J].Biochem Biphys. 1998,356:133-141
131. Kameoka S,Leavitt P, Chang C,et al.Expression of antioxidant proteins inhuman Caco-2 cells treated with dietary flavonoids[J].Cancer Lett. 1999,146:161-167
132. Chan W H,Yu J S.Inhibition of irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermal carcinoma A431 cells by genistein[J].Cell Biochem.2000,78:73-84
133.刘丽,金宏.大豆异黄酮抗氧化作用的研究进展[J].中华放射医学与防护杂志,2003,23(2):132-134
134. Messina M J,Persky V, Setchell K D R,et al.Soy intake and cancer risk:a review of the in vitro and in vivo data[J].Nutr Cancer. 1994,21:113
135. Pollard M,Luckert P H.Influence of isoflavones in soy protein isolates on development of induced prostate-related cancers in L-W rats[J]. Nutr Cancer. 1997, 28(1): 41
136. Zeming J, Macdonald R S. Soy isoflavones latency of spontaneous mammary tumors in mice[J]. Nutr. 2002, 132: 3186-3190
137. Barnes R, Petrson T G, Grubbs C. In diet and Cancer: Markers, prevention and treatment. New York Plenum Press. 1994, 135
138. Sharma O P, Grubbs C, Setchell K D, et al. Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats[J]. Steroid Biochem Mol Biol. 1992, 43(6): 557-564
139.杨峥嵘,程天民,何飞.genistein对人乳腺癌抑瘤效应及造血辐射防护的实验研究[J].中华肿瘤杂志,2002,24(6):548
140. Verma S P, Goldin B R, Lin P S, The inhibition of the estrogenic effects of pesticides and environmental chemicals by curcumin and isoflavonoids[J]. Environmental Health Perspect. 1998, 106(12): 807-812
141. Nakagava H, Yamamoto D, Kiyozuka Y, etal. Effects of genistein and synergistic action in combination with eicosapentaenoic acid on the growth of breast cancer cell lines[J]. Cancer Res Clin Oncol. 2000, 126(8): 448-454
142. Constantinou A I, Krygier A E, Metha R R. Genistein includes maturation of cultured human breast cancer cells and prevents tumor growth in nude mice[J]. Clin Nutr. 1998, 68(6 Supple): 1426S-1430S
143. Allred C D, Allred K F, Ju Y H, etal. Soy diets containing varying amounts of genistein stimulate growth of estrogen-dependent (MCF-7) tumors in a dose-dependent manner[J]. Cancer Res. 2001, 61(13): 5045-5050
144. Ju Y H, Allred C D, Allred K F, etal. Physiological concentrations of dietary genistein dose-dependently stimulate growth of estrogen-dependent human breast cancer (MCF-7) tumors imolanted in athymic nude mice[J]. Nutr. 2001, 131(11): 2957-2962
145.李咏梅,厉曙光.大豆异黄酮抗癌作用的研究进展[J].同济大学学报,2002,23(4):351-353
146.谢明杰等.大豆异黄酮生理功能的研究进展[J].食品与发酵工业,2004,30(5):94-98
147.杨镇洲.大豆异黄酮的抗癌效应研究进展[J].国外医学肿瘤学分册,2001,28(2):107-110
148.王晓明,付国财,王震武.大豆甙元取代饲料中抗生素添加剂饲喂仔猪试验[J].湖北农业科学,2002,1:69-70
149.黄进,罗琼,李晓莉等.大豆异黄酮的降血糖活性研究[J].食品科学,2004,25(1):1-5
150.郭舜民,郭尧惠.天然药降血糖成分的研究进展[J].海峡药学,2000,12(1):1-5
151.王林山,陈月英.大豆异黄酮的研究进展[J].食品研究与开发,2004,25(5):14-17
152. Michael Nain, Benjamin Gesterner, Shmuel Zilkah, etal. Soybean isoflavones characterization, determination and antifungal activity[J]. Agri Food Chem. 1974, 22(5): 806-810
153.谢明杰,陆敏,邹翠霞等.大豆异黄酮的抑菌作用[J].大豆科学,2004,23(2):101-105
154.刘刚,刘东,张晓喻.大豆异黄酮对皮肤保水活性的研究[J].西南民族大学学报(自然科学版),2003,29(4):429-430
155.朱建林,杨桂莲,黄忆明.大豆异黄酮对去卵巢小鼠认知功能的影响[J].中南大学学报(医学版),2004,29(1):81-83
156. Weber K S, Setchell K D, Stocco D M, etal. Dietary soy phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5 alpha reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats[J]. Endorcrinal, 2001, 170(3): 591-599
157. Lund T D, Rhees R W, Setchell K D, etal. Altered sexually dimorphic nucleus of the preoptic area (SDN-POA) volume in adult Long Evans rats by dietary soy phytoestrogens[J]. Brain Res. 2001, 914(1-2): 92-99
158. Kwon S M, Kim S I, Chun D C, etal. Development of rat protatitis model by oral administration of isoflavone and its characteristics[J]. Yonsei Med. 2001, 42(4): 395-404
159.任国峰,黄忆明.大豆异黄酮对大鼠前列腺增生的抑制作用[J].湖南医科大学学报,2003,28(4):243-246
160.朱心强.外来雌激素及其对雄性生殖系统发育的影响[J].国外医学卫生学分册,1995,22(4):212-216
161.伍喜林,郭春华.植物性类激素物质的营养作用研究与开发前景[J].畜禽业,2002,9:38-39
162.朱泽远,周维仁,乐国伟.异黄酮植物雌激素对动物生长的影响及其作用机制[J].兽药与饲料添加剂,2003,5:25-27
163.赵志辉,徐银学,陆天水等.大豆黄酮对2周龄仔猪增重及肝脏GH受体的影响[J].上海交通大学学报(农业科学版),2003,21(1):40-43
164.耿志荣.大豆黄酮和染料木素的RP-HPLC方法的建立及其在禽类体内蛋内含量的研究.南京农业大学硕士论文,2003
165. Han Z K, Wang G J. Studies of phytoestrogens(Daidzein) affecting on growth and sex hormone levels in broiler. ⅦWorld conference on animal production, Edmonton Cananda, 1993: 152-153
166.王国杰,韩正康,陈杰等.大豆黄酮对肉鸡生长的影响及其作用机制的研究[J].广东畜牧兽医科技,1994,19(3):4-6
167.李斌斌,于世凤.Genistein对破骨细胞分化和骨吸收功能的影响及其机制探讨[J].中国骨质疏松杂志,2004,10(3):272-276
168.张宏昱,张石革.依普黄酮的研究进展与临床应用[J].中国医院用药评价与分析,2002,2(6):363-364
169.柯叶艳,王国杰,韩正康.日粮中添加大豆黄酮对鹌鹑生产性能和血液中激素水平的影响[J].畜牧 兽医学报,2002,33(3):243-246
170. Cogburn L A, Nien-chen Mao, etal. Interaction between somatotropic and thyrotropic axes in regulation of growth and development of broiler chickens[J]. European Poultry Science. 1995, E20093: 18-21
171.赵茹茜,周玉传,徐银学等.大豆黄酮对高邮鸭增重及血清某些激素水平和垂体mRNA表达的影响[J].农业生物技术学报,2002,10(2):176-179
172.杨黎娟.雌激素抗骨质疏松的研究进展(综述)[J].国外医学内科学分册,2004,31(2):84-87
173.周振雷,侯加法.雌激素对骨代谢的调节作用(综述)[J].中国临床康复,2004,8(14):2714-2715
174.马学会,武现军,倪耀娣等.大豆黄酮对产蛋鸡蛋壳品质和骨骼代谢的影响[J].饲料工业,2004,25(7):32-34
175.王媛,常红,黄国伟等.大豆异黄酮对体外原代大鼠成骨细胞增殖、分化的影响[J].天津医科大学学报,2004,10(1):53-55
176.王建华,佟晓旭,陈永春等.大豆甙元对大鼠成骨细胞碱性磷酸酶活性的影响[J].中药药理与临床,2003,19(4):8-9