毛竹实生苗水培体系建立的研究
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摘要
本文以毛竹实生苗为材料,以生物量、叶面积、根冠比、叶绿素荧光特性及光谱反射特征为指标,筛选适宜毛竹实生苗生长的条件,探讨营养液中不同氮、磷、钾的浓度对毛竹实生苗的影响,建立毛竹水培技术体系,为毛竹相关研究提供基础平台。主要研究结果如下:
1. Hoagland,MS,Yoshida营养液筛选试验表明,对照增加生物量与Yoshida处理没有显著差异,但显著大于Hoagland和MS处理。3个处理中Yoshida处理叶面积和根冠比最大,MS和Hoagland依次降低。Yoshida处理的叶绿素荧光参数和光谱特征都优于其他处理。综合比较得出,Yoshida营养液配方较适宜毛竹实生苗的生长。
2. 1/4 Yoshida,1/2 Yoshida,Yoshida,3/2 Yoshida筛选试验表明,随着离子强度的增加,生物量、叶面积、根冠比表现为先增加后降低的趋势,1/2 Yoshida处理达到峰值。1/2 Yoshida处理叶绿素荧光参数和光谱特征都优于其他处理,其中Fm、Fv、Fv/Fm、Fv/Fo极显著大于其他3个处理。综合比较得出,1/2 Yoshida处理较优。
3.不同pH梯度筛选试验表明,pH5.0处理增加的生物量最大,显著大于pH4.0、pH4.5、pH5.5和pH6.0处理;叶面积极显著大于其他4个处理。而根冠比差异不明显。pH5.0处理叶绿素荧光参数和光谱特征都优于其他处理,其中Fm、Fv、Fv/Fm、Fv/Fo极显著大于其他的4个处理。综合比较得出,pH5.0为适宜的pH值。
4.不同更新营养液时间间隔和不同通气时间筛选试验表明,7d更新一次营养液各指标优于3d、10d、15d处理。24h通气处理各指标优于通气8h和通气16h处理。综合比较得出,更新营养液时间7d,24h通气为较优更新营养液时间间隔和通气时间。
5.氮、磷、钾单因素试验。氮素浓度筛选试验表明,氮素浓度3mM处理各指标优于1mM、2mM、4mM、5mM处理;磷素浓度筛选试验表明,磷素浓度1mM处理优于0.5mM、1.5mM、2mM处理;钾素浓度筛选试验表明,钾素浓度1.5mM处理优于0.5mM、1mM、2mM、2.5mM处理。
6.氮、磷、钾三因素三水平试验L9(3~3)表明,氮素4mM,磷素0.5mM和钾素1mM为较适宜毛竹实生苗生长的浓度配比。
Solution culture experiments were carried out on moso bamboo seedlings to establish a solution culture protocol. By compared of increased biomass, leaf-area, root/shoot, chlorophyll fluorescence characteristics and reflectance spectra characteristics, we selected optimal conditions, studied the effect of different N, P, K concentration in solution .The main findings are as follows:
1. Experiment of selecting different formula of nutrient liquid treatments indected that the icreased biomass of CK similar to Yoshida treatment but significantly higher than Hoagland and MS treatments. The leaf-area, root/shoot, Chlorophyll fluorescence parameters and reflectance spectra characteristics of Yoshida treatment were superior to Hoagland and MS treatments. So, Yoshida formula of nutrient is more suitable for growth of moso bamboo seedlings.
2. Experiment of selecting 1/4 Yoshida, 1/2 Yoshida, Yoshida, 3/2 Yoshida indected that with the increase of ionic strength the increased biomass, leaf-area and root/shoot performanced increased first and then decreased.1/2 Yoshida handling the peak. Chlorophyll fluorescence parameters and reflectance spectra characteristics of 1/2 Yoshida treatment were superior to other treatments, moreover Fm, Fv, Fv/Fm, Fv/Fo were very significantly. So, 1/2 Yoshida is better.
3. Experiment of solution pH indected that the increased biomass of pH5.0 treatment significantly higher than pH4.0, pH4.5, pH5.5 and pH6.0 treatments.while leaf-area is very significantly higher. But root/shoot has no difference between treatments. Chlorophyll fluorescence parameters and reflectance spectra characteristics of 1/2 Yoshida treatment were superior to other treatments, meanwhile Fm, Fv, Fv/Fm, Fv/Fo were very significantly. So, the nutrient solution of pH5.0 is most suitable for growth of moso bamboo seedlings.
4. Experiment of selecting interval of renew nutrition solution and optimal aeration time. By considering for the indexes of increased biomass, leaf-area, root/shoot, Chlorophyll fluorescence parameters and reflectance spectra characteristics, the renew nutrition solution per week was superior to 3d, 10d, 15d treatments.and aeration time 24h per day was superior to 8h and 16h treatments. So the interval of renew nutrition solution per week and aeration time 24h per day are most suitable for growth of moso bamboo seedlings.
5. N, P, K univariate experiments. Experiment of selecting nitrogen concentration indicted that the indexes of 3mM treatment were superior to 1mM, 2mM, 4mM and 5mM treatments. Experiment of selecting phosphorus concentration indicted that the indexes of 1mM were superior to 0.5mM, 1.5mM and 2mM treatments. Experiment of selecting potassium concentration indicted that the indexes of 1.5mM superior to 0.5mM、1mM、2mM and 2.5mM treatments.
6. N, P, K Orthogonal Test (L9(3~3)) shows that the optimum N, P, K levels were as follows:4mM nitrogen, 0.5mM phosphorus, and 1mM potassium were the best for the seedling growth.
1. Hoagland,MS,Yoshida营养液筛选试验表明,对照增加生物量与Yoshida处理没有显著差异,但显著大于Hoagland和MS处理。3个处理中Yoshida处理叶面积和根冠比最大,MS和Hoagland依次降低。Yoshida处理的叶绿素荧光参数和光谱特征都优于其他处理。综合比较得出,Yoshida营养液配方较适宜毛竹实生苗的生长。
2. 1/4 Yoshida,1/2 Yoshida,Yoshida,3/2 Yoshida筛选试验表明,随着离子强度的增加,生物量、叶面积、根冠比表现为先增加后降低的趋势,1/2 Yoshida处理达到峰值。1/2 Yoshida处理叶绿素荧光参数和光谱特征都优于其他处理,其中Fm、Fv、Fv/Fm、Fv/Fo极显著大于其他3个处理。综合比较得出,1/2 Yoshida处理较优。
3.不同pH梯度筛选试验表明,pH5.0处理增加的生物量最大,显著大于pH4.0、pH4.5、pH5.5和pH6.0处理;叶面积极显著大于其他4个处理。而根冠比差异不明显。pH5.0处理叶绿素荧光参数和光谱特征都优于其他处理,其中Fm、Fv、Fv/Fm、Fv/Fo极显著大于其他的4个处理。综合比较得出,pH5.0为适宜的pH值。
4.不同更新营养液时间间隔和不同通气时间筛选试验表明,7d更新一次营养液各指标优于3d、10d、15d处理。24h通气处理各指标优于通气8h和通气16h处理。综合比较得出,更新营养液时间7d,24h通气为较优更新营养液时间间隔和通气时间。
5.氮、磷、钾单因素试验。氮素浓度筛选试验表明,氮素浓度3mM处理各指标优于1mM、2mM、4mM、5mM处理;磷素浓度筛选试验表明,磷素浓度1mM处理优于0.5mM、1.5mM、2mM处理;钾素浓度筛选试验表明,钾素浓度1.5mM处理优于0.5mM、1mM、2mM、2.5mM处理。
6.氮、磷、钾三因素三水平试验L9(3~3)表明,氮素4mM,磷素0.5mM和钾素1mM为较适宜毛竹实生苗生长的浓度配比。
Solution culture experiments were carried out on moso bamboo seedlings to establish a solution culture protocol. By compared of increased biomass, leaf-area, root/shoot, chlorophyll fluorescence characteristics and reflectance spectra characteristics, we selected optimal conditions, studied the effect of different N, P, K concentration in solution .The main findings are as follows:
1. Experiment of selecting different formula of nutrient liquid treatments indected that the icreased biomass of CK similar to Yoshida treatment but significantly higher than Hoagland and MS treatments. The leaf-area, root/shoot, Chlorophyll fluorescence parameters and reflectance spectra characteristics of Yoshida treatment were superior to Hoagland and MS treatments. So, Yoshida formula of nutrient is more suitable for growth of moso bamboo seedlings.
2. Experiment of selecting 1/4 Yoshida, 1/2 Yoshida, Yoshida, 3/2 Yoshida indected that with the increase of ionic strength the increased biomass, leaf-area and root/shoot performanced increased first and then decreased.1/2 Yoshida handling the peak. Chlorophyll fluorescence parameters and reflectance spectra characteristics of 1/2 Yoshida treatment were superior to other treatments, moreover Fm, Fv, Fv/Fm, Fv/Fo were very significantly. So, 1/2 Yoshida is better.
3. Experiment of solution pH indected that the increased biomass of pH5.0 treatment significantly higher than pH4.0, pH4.5, pH5.5 and pH6.0 treatments.while leaf-area is very significantly higher. But root/shoot has no difference between treatments. Chlorophyll fluorescence parameters and reflectance spectra characteristics of 1/2 Yoshida treatment were superior to other treatments, meanwhile Fm, Fv, Fv/Fm, Fv/Fo were very significantly. So, the nutrient solution of pH5.0 is most suitable for growth of moso bamboo seedlings.
4. Experiment of selecting interval of renew nutrition solution and optimal aeration time. By considering for the indexes of increased biomass, leaf-area, root/shoot, Chlorophyll fluorescence parameters and reflectance spectra characteristics, the renew nutrition solution per week was superior to 3d, 10d, 15d treatments.and aeration time 24h per day was superior to 8h and 16h treatments. So the interval of renew nutrition solution per week and aeration time 24h per day are most suitable for growth of moso bamboo seedlings.
5. N, P, K univariate experiments. Experiment of selecting nitrogen concentration indicted that the indexes of 3mM treatment were superior to 1mM, 2mM, 4mM and 5mM treatments. Experiment of selecting phosphorus concentration indicted that the indexes of 1mM were superior to 0.5mM, 1.5mM and 2mM treatments. Experiment of selecting potassium concentration indicted that the indexes of 1.5mM superior to 0.5mM、1mM、2mM and 2.5mM treatments.
6. N, P, K Orthogonal Test (L9(3~3)) shows that the optimum N, P, K levels were as follows:4mM nitrogen, 0.5mM phosphorus, and 1mM potassium were the best for the seedling growth.
引文
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