光束轨道角动量的理论研究和光镊分选细胞的实验研究
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摘要
光镊又称单光束梯度力阱,是由高度会聚的激光束形成的,可以捕获从几纳米到几十微米的生物大分子微粒、细胞器等,并在不影响周围环境的情况下对捕获物进行非接触性、无损伤的活体操作。光致旋转可实现对微粒的角向操纵,这是在光镊对微粒的三维操作基础上又增加了一维角向的操纵,光束的轨道角动量已成为目前的研究热点。
课题的内容包括光镊及光致旋转的原理,光镊对毛细管中的生物细胞的微操纵与光阱力的测量,利用光镊精确分选酵母菌细胞,光束的轨道角动量理论及高斯涡旋光束的轨道角动量密度的模拟。
论文首先从电磁场理论出发推导了一般光束的轨道角动量密度的公式,利用高斯涡旋光束的电场强度公式并结合推导出的轨道角动量密度公式得到了高斯涡旋光束的轨道角动量密度的表达式,运用MATLAB程序模拟了高斯涡旋光束的光场分布及其轨道角动量密度分布。为高斯涡旋光束的轨道角动量应用于光学微操控领域做了前期理论分析工作。
其次,利用已搭建的光镊实验平台进行了毛细管中生物细胞的光学微操纵实验研究,根据实验得出的数据,利用流体力学方法计算了酵母菌细胞的光阱力大小。
最后,在光学微操纵的基础上设计了光镊精确分选生物细胞的实验,并且在光镊实验平台上,成功地分选出了目标细胞。
Optical tweezers as a optical trap, which cause of highly compact laser beam, could capture biological large molecules particles, cell vehicles from several nm to tensμm, and does not affect surroundings of the catch, a non-contact, non-destructive bacterial operation. Optical rotation can operate particle on the angle, This adds the one-dimensional angle operation on the basis of three-dimensional operation. The orbital angular momentum of beam has now become one of the research hot spot.
This issue include the theory of optical tweezers and optical rotation; optical tweezers micromanipulation biological cell in capillary and measure optical trapping force; Apply optical tweezers accurate sort microzyme; The theory of beam orbital angular momentum and the simulation of orbital angular momentum of Gauss votex beam.
Firstly, the issue according to the theory of electromagnetic field, the orbital angular momentum density formula of a general beam was deduced; from the electric field intensity of a Gauss Vortex Beam, the orbital angular momentum density calculation formula of a Gaussian vortex beam was also deduced. The MATLAB program was applied to simulate the optical field distributions and the orbital angular momentum density distributions of Gauss Vortex Beams. Earlier theoretical analyses have been done for orbital angular momentum of Gaussian vortex beams applied to the optical micromanipula- tions.
Secondly, the issue discussed the component, technical parameters of basically optical tweezers platform from optical tweezers platform that have set up. On this optical tweezers platform the experiment of optical micromani- pulate biological cell in capillary is researched, and calculated optical trapping force apply hydromechanics method on the basis of experiment data.
In the end, based on optical micromanipulation optical accurate sort biological cell experiment is designed, and successivelly sortted target cell on the optical tweezers platform.
课题的内容包括光镊及光致旋转的原理,光镊对毛细管中的生物细胞的微操纵与光阱力的测量,利用光镊精确分选酵母菌细胞,光束的轨道角动量理论及高斯涡旋光束的轨道角动量密度的模拟。
论文首先从电磁场理论出发推导了一般光束的轨道角动量密度的公式,利用高斯涡旋光束的电场强度公式并结合推导出的轨道角动量密度公式得到了高斯涡旋光束的轨道角动量密度的表达式,运用MATLAB程序模拟了高斯涡旋光束的光场分布及其轨道角动量密度分布。为高斯涡旋光束的轨道角动量应用于光学微操控领域做了前期理论分析工作。
其次,利用已搭建的光镊实验平台进行了毛细管中生物细胞的光学微操纵实验研究,根据实验得出的数据,利用流体力学方法计算了酵母菌细胞的光阱力大小。
最后,在光学微操纵的基础上设计了光镊精确分选生物细胞的实验,并且在光镊实验平台上,成功地分选出了目标细胞。
Optical tweezers as a optical trap, which cause of highly compact laser beam, could capture biological large molecules particles, cell vehicles from several nm to tensμm, and does not affect surroundings of the catch, a non-contact, non-destructive bacterial operation. Optical rotation can operate particle on the angle, This adds the one-dimensional angle operation on the basis of three-dimensional operation. The orbital angular momentum of beam has now become one of the research hot spot.
This issue include the theory of optical tweezers and optical rotation; optical tweezers micromanipulation biological cell in capillary and measure optical trapping force; Apply optical tweezers accurate sort microzyme; The theory of beam orbital angular momentum and the simulation of orbital angular momentum of Gauss votex beam.
Firstly, the issue according to the theory of electromagnetic field, the orbital angular momentum density formula of a general beam was deduced; from the electric field intensity of a Gauss Vortex Beam, the orbital angular momentum density calculation formula of a Gaussian vortex beam was also deduced. The MATLAB program was applied to simulate the optical field distributions and the orbital angular momentum density distributions of Gauss Vortex Beams. Earlier theoretical analyses have been done for orbital angular momentum of Gaussian vortex beams applied to the optical micromanipula- tions.
Secondly, the issue discussed the component, technical parameters of basically optical tweezers platform from optical tweezers platform that have set up. On this optical tweezers platform the experiment of optical micromani- pulate biological cell in capillary is researched, and calculated optical trapping force apply hydromechanics method on the basis of experiment data.
In the end, based on optical micromanipulation optical accurate sort biological cell experiment is designed, and successivelly sortted target cell on the optical tweezers platform.
引文
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4 A. Ashkin. Acceleration and Trapping of Particles by Radiation Pressure. Phys. Rev. Lett., 1970,(24):156-159
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