亚麻离体再生及早期体细胞胚胎发生机理的研究
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摘要
亚麻(Linum usitatissimum L.)是一种重要的经济作物,亚麻茎杆可以剥取高档纤维用于纺织业,籽粒可以榨制富含高不饱和脂肪酸的营养保健油品。黑龙江省是我国纤维亚麻的主要产区,随着人们生活水平的提高,发展亚麻生产显得越来越重要。本文选择特点各异的国产品种黑亚14和引进品种Opaline为代表,对纤维亚麻在器官发生和体细胞胚胎发生过程中的组织细胞学、生理生化特性、内源激素含量变化、RAPD标记进行了研究,以阐述其发生机理。研究结果如下:
1.对亚麻器官发生途径的培养条件进行了优化,试验得出了不同基因型无菌苗的最佳消毒时间,10-15日龄的无菌苗活性较好,亚麻下胚轴是诱导愈伤组织最理想的取材部位,平放外植体对出愈最有利。器官发生途径的初始愈伤组织诱导的最佳培养基为MS+4mg/L IAA +2mg/L KT;诱导不定芽发生的最佳培养基为B5+2.5mg/L KT +1mg/L IAA;出苗后最佳生根诱导培养基为1/2B5+0.3mg/L IBA。初始愈伤组织诱导周期为20d左右,呈淡绿色,质地致密坚硬。不定芽诱导周期为30d,IAA与KT是诱导亚麻愈伤组织出芽的关键物质。本实验中基因型间生长趋势差异明显,添加1.5g/L的活性炭可降低愈伤组织褐化率。
2.以体胚发生为目的的亚麻初始愈伤组织的诱导,需要高浓度的2,4-D和较低浓度6-BA,但是过高浓度的2,4-D会抑制出愈和愈伤组织的生长,最佳诱导培养基为MS+2mg/L 2,4-D +0.5mg/L 6-BA;体细胞胚胎诱导培养基为MS+0.5mg/L NAA +0.5mg/L 6-BA;胚性愈伤组织大多呈黄白色,小颗粒状、松散易剥离,表面湿润。基因型对体胚发生影响明显,本实验只在H14体胚诱导中得到了球形胚和心形胚。另外光的变化对亚麻体胚发生没有影响。
3.组织细胞学研究表明,亚麻胚性愈伤组织细胞规则,细胞核大,细胞质浓,非胚性愈伤组织细胞体积大,细胞核小,细胞质稀薄。体细胞胚发生经历了胚性细胞团、2细胞、3细胞、多细胞原胚、球形胚、心形胚发育阶段,但是没有更远的发展。PAS反应中,H14外植体下胚轴的横切可见到皮层内侧有淀粉粒分布,OP外植体子叶的横切可观察到栅栏组织细胞有淀粉粒分布;H14和OP初始愈伤组织外缘淀粉粒较多。H14和OP胚性愈伤组织胚性细胞含有大量的淀粉粒,与细胞的进一步分裂和分化提供能量有关。
4.透射电镜观察表明,初始愈伤组织细胞有非常明显的中央大液泡,细胞质被中央大液泡挤压成一薄层分布于细胞的边缘,细胞核被大液泡挤压,位于细胞的一角,但仍可观察到核仁的存在。胚性愈伤组织细胞壁与初始愈伤组织细胞壁相比加厚,细胞质浓厚,充满整个细胞,细胞核大,核仁明显,在靠近细胞膜的胞质区域里有较多的淀粉粒,还可观察到其内含有淀粉粒的叶绿体。非胚性愈伤组织细胞几乎完全为中央大液泡所占据,细胞质被挤压在四周,很难观察到细胞核和其它细胞器。褐化组织细胞器崩解基本没有细胞器。扫描电镜观察表明,亚麻初始愈伤组织表面较质密,细胞之间排列紧密,没有空隙;胚性愈伤组织表面较光滑,细胞形成团粒结构,形成团粒的细胞之间结合紧密,团粒与团粒之间结合松散,可观察到大的空隙,细胞形状较规则。非胚性和褐化愈伤组织表面形状不规则,有较多的覆盖物,呈片状,细胞形状不规则,体积较大。
5.在胚性愈伤中的CAT、POD、可溶性蛋白、核酸和可溶性糖含量均高于非胚性愈伤组织,这些物质含量和组分的变化可能与胚性的维持和细胞的旺盛分裂分化有关。CAT、可溶性糖在无菌苗含量最高,CAT、核酸、可溶性蛋白、可溶性糖在褐化组织中最低,可以说明褐化愈伤组织的生理状态。POD在初始愈伤中含量最高,这与机械性损伤有关;SOD在无菌苗和不定芽期含量高,初始愈伤组织中的含量是最低的,与其抗氧化能力密切相关;可溶性糖等指标在原胚和球形胚时期均呈上升趋势,为体胚发生作好物质和能量上的准备。
6.SDS-PAGE结果显示,非胚性愈伤组织蛋白染色非常浅,蛋白代谢非常弱。亚麻体胚发生各时期的主要蛋白质组分相似,体现了体胚蛋白质组分的稳定性。H14各个阶段的蛋白质范围约为24-94kD,OP各个阶段的蛋白质范围约为20-100kD。在亚麻体细胞胚胎发生早期,共出现了9条POD同工酶酶带,其中有6条带是在亚麻体胚发生早期各阶段陆续出现的,可以作为体细胞胚发生的生化标记。淀粉同工酶在亚麻体胚发生早期出现的条带很少,酶带在非胚性和褐化组织中较多,而且淀粉同工酶的条带数也因品种不同而不同。亚麻早期体胚发生过程中,各个阶段EST同工酶酶带颜色由深到浅,这与POD同工酶酶带颜色和淀粉同工酶酶带颜色的变化相反。
7.内源激素水平和激素间的平衡对亚麻形态建成具有重要的调节作用。胚性愈伤组织中的IAA含量高于非胚性愈伤组织,而且在原胚期和球形胚时期都保持了较高的水平,褐化组织中的IAA含量最低。ABA在外植体中的含量最高,在原胚和球形胚时期较低,低含量水平的ABA有利于胚的形成。ZR的峰值出现在初始愈伤中,胚性愈伤中的ZR含量低于非胚性愈伤。胚性愈伤中的IAA/ZR远高于非胚性愈伤,这可能有利于胚性能力的表达。
8.本实验中,亚麻染色体数2n=32,继代5次以后变异率为2.5%,继代9次后变异率为4.3%,既有数量变异,也观察到有染色体结构的变异。经RAPD标记,发现引物S10、S21、S24和S48扩增产物的电泳结果中,褐化组织比无菌苗及其它时期少一条带,说明在继代过程中(到褐化组织时),亚麻的基因组DNA发生了变异。
Flax (Linum usitatissimum L.) is one of important economic crops, from flax stalk we can obtain high-grade fiber for textiles, and from seeds we can obtain high-grand oil which have rich unsaturated fatty acids. Heilongjiang province is one main production area of fibre flax in China. Along with the improvement of people’s living standard, it is gradually important to develop flax production. Two varieties, H14(domestic) and Opaline(foreign) were selected as test materials to study on organization cytology, characteristics of physiology and biochemistry, changes of endogenous hormones content and RAPD markers during organogenesis and somatic embryogenesis of flax, and explain its occurrence mechanism. Research results are as follows.
1. Culture conditions for organogenesis in flax were optimized, we obtained optimal sterilization time of aseptic plantlet with different genotype, aseptic plantlet with 10-15d had good activity. Furthermore, flax hypocotyl was optimal material for callus induction, and the better position is horizontal onto the medium. The prime medium of initial callus induction was MS+4mg/L IAA +2mg/L KT, and the best medium of adventitious buds induction during organogenesis was B5+2.5mg/L KT +1mg/L IAA. The best rooting induction medium was 1/2B5+0.3mg/L IBA. Induction duration was 20d or so, and initial callus were weak green, compact and stiff. The adventitious buds induction period was about 30d. IAA and KT were key factor to induce bud of flax callus. There are different in growth of callus among genotypes. 1.5g/L activate carbon could lower the browning ratio of callus.
2. For somatic embryogenesis,the initial callus induction of flax needed high concentration 2,4-D and low concentration 6-BA. but overabundance 2,4-D could inhibit induction and growth of callus, the prime induction medium was MS+2mg/L 2,4-D +0.5mg/L 6-BA. The induction medium of somatic embryogenic was MS+0.5mg/L NAA +0.5mg/L 6-BA. Mostly embryogenic callus were yellow-white, granule, incompact and wetting surface. Genotype had evident effects on somatic embryogenesis. We only gained globular and heart-shaped embryo in H14. Changes of light had not effect on somatic embryogenesis in flax.
3. It was showed by cytology method that flax embryogenic callus consisted of regular shaped cells with dense cytoplasm, large nucleus while the nonembryogenic callus cells with large volume, small nucleus and shin cytoplasm. Embryogenic cell mass, 2-cell, 3-cell, proembryogenic masses, initial spherical embryo and hearted embryo were observed, but Somatic embryogenesis had not further development. It showed that the medial cortex had starch grains distribution in PAS reaction from cross section of H14 hypocotyls. Starch grains distribution in palisade tissue cells of cross section of OP cotyledon were observed. There were plenty of starch grains in outside cell of initial callus of H14 and OP. Embryogenic cell of H14 and OP accumulated large number of starch grains which might supply energy for cell division and differentiation. 4. It was showed by transmission electron microscope that the initial callus cells had large central vacuole, which squeezed its cytoplasm to a thin layer around the brim of cell. Nuclear distributed the corner of cells because of being squeezed by large vacuole, however we found the presence of nuclear. Compared embryogenic callus with initial callus its cell wall became thick, it had dense cytoplasm which was full of the whole cell, big nucleus with obvious nucleolus. Many starch grains and chloroplasts including starch grains distributed on the cytoplasm area of cell membrane. The cells of nonembryogenic callus were almost occupied by central large vacuole, and its cytoplasm was extruded. Nucleus and other organelles were hardly observed. Browning organelle had breakdown and had not organelle at all. As showed as followed by Scanning electron microscope that flax initial callus owned dense surfaces and there was little gap among cells, which shared silk-shaped attachment in the cell surface. On the contrary, embryogenic callus had smooth surfaces, and the embryogenic cells were closely conjuncted to form cell masses in which loosely connection and large empty were observed. The cells of cell masses were regular- shaped, and many of them exposed on the surface of embryogenic callus. Nonembryogenic callus and browning callus surface shape was irregular and covered with lamellar. They had different shape with large volume.
5. The content of CAT, POD, soluble protein, nucleic acids and soluble sugar in embryogenic callus were higher than in nonembryogenic callus, these substance changes of content and components may be relevanted to maintain of embryogenic and hearty splitting and differentiation of cells. The content of CAT and soluble sugar was maximum in aseptic plantlet, the content of CAT, nucleic acids, soluble protein and soluble sugar callus were all lowest in browning tissue, which showed the physiological state of browning callus. The POD content was highest in initial callus tissue, this was relevant to mechanical damage. The SOD content was high in the aseptic plantlet and adventitious buds, and lowest in initial callus, this was relevant closely to antioxidant ability. The content of soluble sugar ect were gradually increased in proembryogenic and spherical embryogenic, which were prepared for substance and energy of somatic embryogenesis.
6. The results of SDS-PAGE indicated that the protein staining of nonembryogenic callus was very light, and the metabolism of protein was very weak. The main protein components of each stage of flax somatic embryogenesis were very similar, which means the stability of somatic embryogenic components. The protein scope of each stage for H14 were 24-94kD, and 20-100kD for OP. It presented 9 POD isozyme strips during the initial somatic embryogenesis, 6 of those were presented continuously during the initial somatic embryogenesis, which were used for the biochemical markers of the initial somatic embryogenesis. The amylase isozyme strips were less in the initial somatic embryogenesis, nonembryogenic and browning callus, and more in nonembryogenic and browning callus, the number of amylase isozyme strips varied with different varieties. The EST isozyme strips color varied from deep to light during the stages of flax initial somatic, which is opposite to the variety of POD isozyme strips and starch isozyme strips.
7. The level and the balance of endogenous hormones can adjust to flax morphogenesis. The content of IAA in embryogenic callus was higher than in nonembryogenic callus, and it maintained high level in proembryogenic period and globular embryogenic period, its content was lowest in browning callus. The content of ABA was highest in explant, its content was less in proembryogenic period and globular embryogenic period, low level of ABA was benefited to the embryogenesis. The peak value of ZR content present to initial callus, the ZR content of embryogenic callus was lower than in nonembryogenic callus, its content was the lowest in browning callus. The IAA/ZR ratio of embryogenic callus was far higher than in nonembryogenic callus, it may be benefit to the expression of embryogenic capability.
8. The number of flax chromosome were 2n=32, the variation rate was 2.5% after 5 subculture, and 4.3% after 9 subculture in this test. It had quantity variation and the chromosome structure variation. According to RAPD markers, it was found that one strip had lost in browning callus compared with aseptic plantlet and other periods in the amplification product of S10、S21、S24 and S48, it was discerned that the genome DNA of flax had changed during subculture(add to browning callus).
1.对亚麻器官发生途径的培养条件进行了优化,试验得出了不同基因型无菌苗的最佳消毒时间,10-15日龄的无菌苗活性较好,亚麻下胚轴是诱导愈伤组织最理想的取材部位,平放外植体对出愈最有利。器官发生途径的初始愈伤组织诱导的最佳培养基为MS+4mg/L IAA +2mg/L KT;诱导不定芽发生的最佳培养基为B5+2.5mg/L KT +1mg/L IAA;出苗后最佳生根诱导培养基为1/2B5+0.3mg/L IBA。初始愈伤组织诱导周期为20d左右,呈淡绿色,质地致密坚硬。不定芽诱导周期为30d,IAA与KT是诱导亚麻愈伤组织出芽的关键物质。本实验中基因型间生长趋势差异明显,添加1.5g/L的活性炭可降低愈伤组织褐化率。
2.以体胚发生为目的的亚麻初始愈伤组织的诱导,需要高浓度的2,4-D和较低浓度6-BA,但是过高浓度的2,4-D会抑制出愈和愈伤组织的生长,最佳诱导培养基为MS+2mg/L 2,4-D +0.5mg/L 6-BA;体细胞胚胎诱导培养基为MS+0.5mg/L NAA +0.5mg/L 6-BA;胚性愈伤组织大多呈黄白色,小颗粒状、松散易剥离,表面湿润。基因型对体胚发生影响明显,本实验只在H14体胚诱导中得到了球形胚和心形胚。另外光的变化对亚麻体胚发生没有影响。
3.组织细胞学研究表明,亚麻胚性愈伤组织细胞规则,细胞核大,细胞质浓,非胚性愈伤组织细胞体积大,细胞核小,细胞质稀薄。体细胞胚发生经历了胚性细胞团、2细胞、3细胞、多细胞原胚、球形胚、心形胚发育阶段,但是没有更远的发展。PAS反应中,H14外植体下胚轴的横切可见到皮层内侧有淀粉粒分布,OP外植体子叶的横切可观察到栅栏组织细胞有淀粉粒分布;H14和OP初始愈伤组织外缘淀粉粒较多。H14和OP胚性愈伤组织胚性细胞含有大量的淀粉粒,与细胞的进一步分裂和分化提供能量有关。
4.透射电镜观察表明,初始愈伤组织细胞有非常明显的中央大液泡,细胞质被中央大液泡挤压成一薄层分布于细胞的边缘,细胞核被大液泡挤压,位于细胞的一角,但仍可观察到核仁的存在。胚性愈伤组织细胞壁与初始愈伤组织细胞壁相比加厚,细胞质浓厚,充满整个细胞,细胞核大,核仁明显,在靠近细胞膜的胞质区域里有较多的淀粉粒,还可观察到其内含有淀粉粒的叶绿体。非胚性愈伤组织细胞几乎完全为中央大液泡所占据,细胞质被挤压在四周,很难观察到细胞核和其它细胞器。褐化组织细胞器崩解基本没有细胞器。扫描电镜观察表明,亚麻初始愈伤组织表面较质密,细胞之间排列紧密,没有空隙;胚性愈伤组织表面较光滑,细胞形成团粒结构,形成团粒的细胞之间结合紧密,团粒与团粒之间结合松散,可观察到大的空隙,细胞形状较规则。非胚性和褐化愈伤组织表面形状不规则,有较多的覆盖物,呈片状,细胞形状不规则,体积较大。
5.在胚性愈伤中的CAT、POD、可溶性蛋白、核酸和可溶性糖含量均高于非胚性愈伤组织,这些物质含量和组分的变化可能与胚性的维持和细胞的旺盛分裂分化有关。CAT、可溶性糖在无菌苗含量最高,CAT、核酸、可溶性蛋白、可溶性糖在褐化组织中最低,可以说明褐化愈伤组织的生理状态。POD在初始愈伤中含量最高,这与机械性损伤有关;SOD在无菌苗和不定芽期含量高,初始愈伤组织中的含量是最低的,与其抗氧化能力密切相关;可溶性糖等指标在原胚和球形胚时期均呈上升趋势,为体胚发生作好物质和能量上的准备。
6.SDS-PAGE结果显示,非胚性愈伤组织蛋白染色非常浅,蛋白代谢非常弱。亚麻体胚发生各时期的主要蛋白质组分相似,体现了体胚蛋白质组分的稳定性。H14各个阶段的蛋白质范围约为24-94kD,OP各个阶段的蛋白质范围约为20-100kD。在亚麻体细胞胚胎发生早期,共出现了9条POD同工酶酶带,其中有6条带是在亚麻体胚发生早期各阶段陆续出现的,可以作为体细胞胚发生的生化标记。淀粉同工酶在亚麻体胚发生早期出现的条带很少,酶带在非胚性和褐化组织中较多,而且淀粉同工酶的条带数也因品种不同而不同。亚麻早期体胚发生过程中,各个阶段EST同工酶酶带颜色由深到浅,这与POD同工酶酶带颜色和淀粉同工酶酶带颜色的变化相反。
7.内源激素水平和激素间的平衡对亚麻形态建成具有重要的调节作用。胚性愈伤组织中的IAA含量高于非胚性愈伤组织,而且在原胚期和球形胚时期都保持了较高的水平,褐化组织中的IAA含量最低。ABA在外植体中的含量最高,在原胚和球形胚时期较低,低含量水平的ABA有利于胚的形成。ZR的峰值出现在初始愈伤中,胚性愈伤中的ZR含量低于非胚性愈伤。胚性愈伤中的IAA/ZR远高于非胚性愈伤,这可能有利于胚性能力的表达。
8.本实验中,亚麻染色体数2n=32,继代5次以后变异率为2.5%,继代9次后变异率为4.3%,既有数量变异,也观察到有染色体结构的变异。经RAPD标记,发现引物S10、S21、S24和S48扩增产物的电泳结果中,褐化组织比无菌苗及其它时期少一条带,说明在继代过程中(到褐化组织时),亚麻的基因组DNA发生了变异。
Flax (Linum usitatissimum L.) is one of important economic crops, from flax stalk we can obtain high-grade fiber for textiles, and from seeds we can obtain high-grand oil which have rich unsaturated fatty acids. Heilongjiang province is one main production area of fibre flax in China. Along with the improvement of people’s living standard, it is gradually important to develop flax production. Two varieties, H14(domestic) and Opaline(foreign) were selected as test materials to study on organization cytology, characteristics of physiology and biochemistry, changes of endogenous hormones content and RAPD markers during organogenesis and somatic embryogenesis of flax, and explain its occurrence mechanism. Research results are as follows.
1. Culture conditions for organogenesis in flax were optimized, we obtained optimal sterilization time of aseptic plantlet with different genotype, aseptic plantlet with 10-15d had good activity. Furthermore, flax hypocotyl was optimal material for callus induction, and the better position is horizontal onto the medium. The prime medium of initial callus induction was MS+4mg/L IAA +2mg/L KT, and the best medium of adventitious buds induction during organogenesis was B5+2.5mg/L KT +1mg/L IAA. The best rooting induction medium was 1/2B5+0.3mg/L IBA. Induction duration was 20d or so, and initial callus were weak green, compact and stiff. The adventitious buds induction period was about 30d. IAA and KT were key factor to induce bud of flax callus. There are different in growth of callus among genotypes. 1.5g/L activate carbon could lower the browning ratio of callus.
2. For somatic embryogenesis,the initial callus induction of flax needed high concentration 2,4-D and low concentration 6-BA. but overabundance 2,4-D could inhibit induction and growth of callus, the prime induction medium was MS+2mg/L 2,4-D +0.5mg/L 6-BA. The induction medium of somatic embryogenic was MS+0.5mg/L NAA +0.5mg/L 6-BA. Mostly embryogenic callus were yellow-white, granule, incompact and wetting surface. Genotype had evident effects on somatic embryogenesis. We only gained globular and heart-shaped embryo in H14. Changes of light had not effect on somatic embryogenesis in flax.
3. It was showed by cytology method that flax embryogenic callus consisted of regular shaped cells with dense cytoplasm, large nucleus while the nonembryogenic callus cells with large volume, small nucleus and shin cytoplasm. Embryogenic cell mass, 2-cell, 3-cell, proembryogenic masses, initial spherical embryo and hearted embryo were observed, but Somatic embryogenesis had not further development. It showed that the medial cortex had starch grains distribution in PAS reaction from cross section of H14 hypocotyls. Starch grains distribution in palisade tissue cells of cross section of OP cotyledon were observed. There were plenty of starch grains in outside cell of initial callus of H14 and OP. Embryogenic cell of H14 and OP accumulated large number of starch grains which might supply energy for cell division and differentiation. 4. It was showed by transmission electron microscope that the initial callus cells had large central vacuole, which squeezed its cytoplasm to a thin layer around the brim of cell. Nuclear distributed the corner of cells because of being squeezed by large vacuole, however we found the presence of nuclear. Compared embryogenic callus with initial callus its cell wall became thick, it had dense cytoplasm which was full of the whole cell, big nucleus with obvious nucleolus. Many starch grains and chloroplasts including starch grains distributed on the cytoplasm area of cell membrane. The cells of nonembryogenic callus were almost occupied by central large vacuole, and its cytoplasm was extruded. Nucleus and other organelles were hardly observed. Browning organelle had breakdown and had not organelle at all. As showed as followed by Scanning electron microscope that flax initial callus owned dense surfaces and there was little gap among cells, which shared silk-shaped attachment in the cell surface. On the contrary, embryogenic callus had smooth surfaces, and the embryogenic cells were closely conjuncted to form cell masses in which loosely connection and large empty were observed. The cells of cell masses were regular- shaped, and many of them exposed on the surface of embryogenic callus. Nonembryogenic callus and browning callus surface shape was irregular and covered with lamellar. They had different shape with large volume.
5. The content of CAT, POD, soluble protein, nucleic acids and soluble sugar in embryogenic callus were higher than in nonembryogenic callus, these substance changes of content and components may be relevanted to maintain of embryogenic and hearty splitting and differentiation of cells. The content of CAT and soluble sugar was maximum in aseptic plantlet, the content of CAT, nucleic acids, soluble protein and soluble sugar callus were all lowest in browning tissue, which showed the physiological state of browning callus. The POD content was highest in initial callus tissue, this was relevant to mechanical damage. The SOD content was high in the aseptic plantlet and adventitious buds, and lowest in initial callus, this was relevant closely to antioxidant ability. The content of soluble sugar ect were gradually increased in proembryogenic and spherical embryogenic, which were prepared for substance and energy of somatic embryogenesis.
6. The results of SDS-PAGE indicated that the protein staining of nonembryogenic callus was very light, and the metabolism of protein was very weak. The main protein components of each stage of flax somatic embryogenesis were very similar, which means the stability of somatic embryogenic components. The protein scope of each stage for H14 were 24-94kD, and 20-100kD for OP. It presented 9 POD isozyme strips during the initial somatic embryogenesis, 6 of those were presented continuously during the initial somatic embryogenesis, which were used for the biochemical markers of the initial somatic embryogenesis. The amylase isozyme strips were less in the initial somatic embryogenesis, nonembryogenic and browning callus, and more in nonembryogenic and browning callus, the number of amylase isozyme strips varied with different varieties. The EST isozyme strips color varied from deep to light during the stages of flax initial somatic, which is opposite to the variety of POD isozyme strips and starch isozyme strips.
7. The level and the balance of endogenous hormones can adjust to flax morphogenesis. The content of IAA in embryogenic callus was higher than in nonembryogenic callus, and it maintained high level in proembryogenic period and globular embryogenic period, its content was lowest in browning callus. The content of ABA was highest in explant, its content was less in proembryogenic period and globular embryogenic period, low level of ABA was benefited to the embryogenesis. The peak value of ZR content present to initial callus, the ZR content of embryogenic callus was lower than in nonembryogenic callus, its content was the lowest in browning callus. The IAA/ZR ratio of embryogenic callus was far higher than in nonembryogenic callus, it may be benefit to the expression of embryogenic capability.
8. The number of flax chromosome were 2n=32, the variation rate was 2.5% after 5 subculture, and 4.3% after 9 subculture in this test. It had quantity variation and the chromosome structure variation. According to RAPD markers, it was found that one strip had lost in browning callus compared with aseptic plantlet and other periods in the amplification product of S10、S21、S24 and S48, it was discerned that the genome DNA of flax had changed during subculture(add to browning callus).
引文
程舟,蛟岛一彦,陈家宽. 2002.红麻种质资源遗传变异和亲缘关系的RAPD分析[J].中国麻业.24(1):1-12.
陈春玲.2001.龙眼体细胞胚胎发生机理的初步研究.福建农林人学硕士学位论文.5.
陈菲.2005.植物组织培养的防褐化探讨[J].北方园艺(2)69-70.
陈婷,李力.2000.水稻悬浮体细胞胚胎发生及其特异蛋白[J].东北师大学报(自然科学版). 32(4):52-58.
陈晓慧.2003.苎麻体系胞胚胎发生与原生质体培养研究.华中农业大学硕士学位论文.
陈秀芳,工坤范.1995.桃果实发育中褐变因子变化规律的研究[J].园艺学报.22:230-234.
陈一峰,周燮,汤日圣等.1998.水稻体细胞培养中胚性细胞出现与IAA的关系植物学报[J]. 40(5):474-477.
崔福君.2003.酿酒葡萄组织培养及再生系统的建立.西北农林科技大学硕士研究生学位论文.
崔凯荣,陈克明,王晓哲.1993.植物体细胞胚发生研究的某些现状[J].植物学通报.10(1):14-20.
崔凯荣,王亚馥.1993.石刁柏组织培养中体细胞胚发生的组织学观察[J].西北植物学报.13:203-206.
崔凯荣,高金成,王亚馥等.1994.石刁柏组织培养中体细胞胚发生的超微结构的研究[J].兰州大学学报(自然科学版)30(增刊):60-65.
崔凯荣,裴新梧,秦琳等.1998.ABA对枸杞体细胞胚发生的调节作用[J].实验生物学报.31(2):195-201.
崔凯荣,戴若兰主编.2000.植物体细胞胚胎发生的分子生物学[M].科学出版社.3.17.
崔凯荣,刑更生,周功克等.2000.植物激素对体细胞胚发生的诱导和调节[J].遗传.22(4): 349-354.
崔凯荣,邢更生,周功克等.2001.体细胞胚发生的生化基础[J].生命科学.1:28-33.
戴均贵,周吉源.1997.华黄茂离体形态发生过程中生理生化特性变化的研究[J].华中师范大学学报:自然科学版.31(2):220-224.
戴若兰,张玮,邢更生等. 1999.拘祀体细胞胚胎发生中蛋白质代谢动态的立体计量C17[J].西北植物学报.19(2):266-269.
邓欣,陈信波,龙松华.2007.10个亚麻品种亲缘关系的RAPD分析[J].中国麻业学.29(4).
杜娟,常国权,季静. 2002.玉米自交系愈伤组织的诱导、RAPD分析及扫描电镜观察[J].作物学报.28(2):282-285.
范国强,黎明,贺窑青等.2004.悬铃木体细胞胚胎发生及植株再生[J].林业科学.40(1): 71-74.
范双喜.1993.植物组织培养中胚状体诱导的研究[J].北京农学院学报.8(2):142-146.
范志辉,孙洪涛等.1997.亚麻体细胞无性系的建立及其植株再生[J].中国麻作.19(1):17-18.
付杨,陈凤清,孙冬雪等.2005.大叶柴胡愈伤组织继代培养及其RAPD分析[J].东北师大学报自然科学版.137:2.
高莉萍,包满珠.2005.月季的植株再生及遗传转化研究进展[J].植物学通报.22(2):231-237.
高国训.1999.植物组织培养中的褐变问题[J].植物生理学通讯.35:501-506.
高述民,陆帼一,杜葱芳.2001.大蒜体细胞胚发育分化中特异蛋白和某些生理生化变化(简报)[J].植物生理学通讯.37(3):207-210.
桂进,桂耀林,郭仲探.1992.三七体细胞胚胎发生及人工种子研制[J].实验生物学报.25(2): 139-147.
郭翔宇.2002.黑龙江省亚麻业的优势与面临的挑战[J].中国麻业.(2):43-45.
郭仲探,陆文楔.1984.玉米花粉愈伤组织产生胚状体的细胞形态学研究[J].植物学报.26(1):19-23.
郭子彪,盖钧镒.1997.内源激素IAA,ABA对大豆萌发子叶胚性愈伤组织诱导及其分化的调控[J].大豆科学.16(3):194-198.
谷瑞升,蒋湘宁,郭仲深.1999.植物离体培养中器官发生调控机制的研究进展[J].植物学通报.16(3): 238-244.
顾玉红,高述民,郭惠红等.2004.文冠果的体细胞胚胎发生[J].植物生理学通讯.40 (3):311-313.
韩碧文,刘淑兰.1988.植物离体体细胞胚胎发生[J].植物生理学通讯.10(1):9-15.
韩碧文.1989.胚状体发生的细胞学和生理生化研究[J].植物学通报.6(1):1-4.
郝再彬,徐仲,苍晶,2002.植物生理实验技术[M].68-69.111-112,123-124,195-197.
郝建平,裴建文,周小梅等.1993.蛇床胚状体发生的扫描电镜观察[J].山西大学学报.16(3): 322-324.
贺红,潘瑞炽,韩美丽等.1998.红江橙体胚发生及影响因素的研究[J].广西植物.18(4): 343-346.
洪岚,叶万辉,沈浩等.2005.薇甘菊组织培养及体细胞胚胎发生的研究[J].浙江大学学报(农业与生命科学版).31(5):572-578.
黄璐,卫志明.1999.同基因型玉米的再生能力和胚性与非胚性愈伤组织DNA的差异[J].植物生理学报.25(4):332-338.
黄素华,赖钟雄.2006.荔枝体细胞胚性培养物的DNA提取方法和RAPD分析[J].热带作物学报.27(4).
黄学林.1995.高等植物离休组织培养的形态建成及其调控[M].北京:科学出版社30-45.
郏艳虹,熊庆娥.植物体细胞胚胎发生的研究[J].四川农业大学学报.2003, 21(3):263-266.
焦德志,李波等.2006.亚麻愈伤组织诱导最佳培养基的筛选[J].安徽农业科学,34(6):1124-1125.
冀艾青.2003.核桃体细胞胚胎发生影响因子的研究.陕西农业大学.硕士研究生学位论文.7.
季道藩主编.1984.普通遗传学[M].北京:中国农业出版社.
揭雨成,周青文,陈佩度.2002.苎麻栽培品种亲缘关系的RAPD分析[J].作物学报.2:254-259.
暨淑仪,严学成等.1995.茶叶片愈伤组织形成的细胞组织学观察.茶叶.21(2)11-13.
姬妍茹,田玉杰等.2001.用组织培养法繁殖野生亚麻的研究[J].中国麻作.23(4):8-11.
姬妍茹,田玉杰.2005.亚麻直接分化再生系统的初步建立[J].生物技术通报.3:41-46.
李代丽.2007.白刺愈伤组织培养中外源激素对内源激素影响的研究.北京林业大学硕士学位论文.6.
李宏潮.1990.小麦成熟胚培养及影响因子的研究.植物细胞工程与育种[M].北京工业大学出版社.308-313.
李军生,李春瑶.1990.甘蔗的植物组织培养[J].广西植物.10(1):75-79.
李文雄,曾寒冰.2006.小麦研究[M].黑龙江科学技术出版社.431-434.
凌定厚,D S Brar,FJ Zapata.1988.籼稻体细胞胚胎发生的组织学及细胞学研究[J].植物学报.30(5):485-489.
林汉章,鲁雪华,陈扬春.1987.非洲紫罗兰胚状体发生的扫描电镜观察[J].福建省农科院学报.2:79-80.
林士杰,姜静,冯昕.2001.黑林1号杨组培叶片不定根的发生与内、外源激素关系的研究[J].林业科技.3(1):8-11.
林娅,郑玉梅,刘青林.2006.影响月季愈伤组织诱导和分化的因素[J].分子植物育种.(6):223-227.
刘涤,迟静芬,刘桂芸.1986.烟草愈伤组织器官发生过程中外源激素的作用[J].植物生理与分子生物学学报.12(1):104-108.
刘华英,萧浪涛,鲁旭东等.2004.柑橘体细胞胚发生的组织细胞学研究[J].果树学报.21(4):311-314.
刘华英,萧浪涛,何长征.2002.植物体细胞胚发生与内源激素的关系研究进展[J].湖南农业大学学报.28(4): 350-354.
刘天磊,江晓雯,王仑山.2002.苜蓿组织培养中球形胚发生时特异蛋白质和同工酶分析[J].西北植物学报.22(3):625-628.
刘瑛,彭渊,赖小萍等,2003.利用组织培养法保存野生苎麻资源的初步研究[J].江西农业学报.15(1),29-32.
刘勇刚,徐子勤,郝建国.2001.温度和水分对不同基因型小麦未成熟胚体细胞胚胎发生以及分化能力的影响[J].西北植物学报.3(5):425-431.
李学宝,陈光荣等.1995.亚麻下胚轴离体培养和转化的研究[J].武汉植物学研究.13(4):344-348.
赖钟雄,陈春玲.2002.龙眼体细胞胚胎发生过程中的内源激素变化[J].热带作物学报,23(2):41-47.
陆敏,黄静.1990.棉枣胚状体发生的扫描电镜观察[J].电了显微学报.9(3):32.
鲁旭东.2004.石刁柏UC800体细胞胚发生系统的建立及其生理生化机理研究.湖南农业大学博士学位论文.11.
马新业.2003.泡桐体细胞胚胎发生及植株再生研究.河南农业大学硕士论文.6.
孟繁静,刘道宏,苏业瑜编著.1992.植物生理生化[M].中国农业大学出版社.259,278.
明凤,李文雄.2000.小麦胚性愈伤组织发生影响因素的研究[J].东北农业大学学报.31(1):1-6.
牛遇达.2007.北五味子体细胞胚发生的研究.东北林业大学硕士学位论文.6.
欧阳学智,谢绍萍,陈睦传等.1997.甜菊愈伤组织分生区细胞中膜内含物的超微结构研究[J].西北植物学报.17(3):326-331.
祁建民,周东新,吴为人等.2004.RAPD和ISSR标记检测黄麻属遗传多样性的比较研究[J].中国农业科学.37(12):2006-2011.
齐力旺,韩一凡,李玲等.2000.华北落叶松体细胞胚发生及遗传转化实验系统的建立[J].实验生物学报.33(4):357-365.
曲春香,奚惕,黄连山.1990.金丝桃的组织培养及快速繁殖[J].植物生理学通讯.(2):40-45.
阙国宁,郭达初,李金田译.1988.树木组织培养[M].北京,中国林业出版社.175,141-143.
屈妹存,刘选明.1998.百合鳞片细胞形态发生中生理生化特性研究[J].生命科学研究.2(4):288-294.
师校欣,杜国强,高仪等.1999.苹果离体叶片高效再生不定芽技术研究[J].果树科学.16(4): 255-258.
宋春凤等.1998.白芷愈伤组织的电镜制样技术及超微结构观察[J].河北师范大学学报.22(3):396-398.
宋仁美. 1985.体细胞胚胎发生.的生化和遗传学研究[J].国外遗传育种.1:31-32.
汤国庆,李文安.1995.在离体条件下拐芹形成胚性愈伤组织和非胚性愈伤组织过程中几种内源激素的变化[J].实验生物学报.28(2):203-208.
王彩云,白吉刚. 2001.桂花的组织培养.北京林业大学学报[J]23(1): 24-25
王关林.方宏筠. 2002.植物基因工程原理与技术[M].科学出版社.742-743.
王家福,刘月学.2000.批把胚性愈伤组织的诱导和保存[J].福建农业大学学报, 29(3):305-310.
汪丽虹,崔凯荣,白志根等.1996.石刁柏非胚性细胞和胚性细胞的超微结构及ATP酶的定位[J].兰州大学学报(自然科学版).32(4):123-127.
汪丽虹,王星,崔凯荣等.1996.石刁柏及党参体细胞胚胎发生中的淀粉代谢动态[J].植物学通报.13(1):41-45.
王洪隆,何俊英,马洪仁远等.1991.大蒜发芽叶愈伤组织细胞超微结构观察与过氧化物酶同工酶的变化[J].华北农学报.6(1):74-80.
王慧梅.2004.喜树组织培养与遗传转化的研究.东北林业大学博士学位论文.6.
王吉之,李克勤.1993.槐树子叶组织培养中的形态发生研究[J].实验生物学报.26(1): 39-49.
王凯基.1979.油橄榄(Dlea,europnea L)组织培养的细胞组织学研究.愈伤组织的建成[J].植物学报,21,(2):127-134.
王丽,鲍晓明,黄百渠等.1998.香雪兰外植体形态学极性决定的体细胞胚胎发生[J].植物学报. 40(2):138-143.
王亚馥,崔凯荣,汪丽虹.1994.小麦体细胞胚胎发生的超微结构研究[J].植物学报.36(6):418-422.
王亚馥,王仑山.1989.枸杞组织培养中形态发生的细胞组织学观察[J].兰州大学学报.25 (4):88-92.
王玉富.2005.我国亚麻生物技术的研究现状及发展[J].中国麻业.27(2):60-65. 王中仁.1996.植物等位酶分析[M].北京科学出版社.10-11.
魏日凤.2006.蔗糖与麦芽糖质量浓度及添加成分对水稻成熟胚培养的效应[J].福建农林大学学报.35(6):565-565.
吴殿星,胡繁荣主编.2004.植物组织培养[M].上海交通大学出版社.
吴绛云. 1981.苹果“黄太平”花药培养获得单倍体植株[J].科学通报.(6)21:1344.
吴永宏,张德颐,王丽蓉. 1993.植物细胞的同步培养技术.植物生理学通讯[J].29(2):108-110
肖关丽,杨清辉,李富生等.2002.甘蔗愈伤组织分化绿苗内源激素变化规律研究[J].西南农业大学学报.24(4):337-338.
肖显华,王庆华,林荣双等.1999.花生体细胞胚胚性特异蛋白的研究.[J].山东烟台大学学报.12(3):185-188.
肖显华,王顺珍,林荣双等.1998.花生成熟胚叶体细胞胚形成过程中的过氧化物酶同工酶研究[J].烟台大学学报.11(1):57-61.
谢海燕,毛碧增,单兰兰等.2004.狗牙根颖果胚性愈伤组织的诱导和胚性细胞的超微结构及植株再生[J].植物生理与分子生物学学报.30(2):209-215.
邢朝斌,沈海龙,刘岩等.2006.刺五加成熟种子的愈伤组织诱导及其RAPD分析[J].种子.25(7):44-49.
邢登辉,赵云云,黄承芬.1999.皇冠草体细胞胚发生及其体胚发生过程中内源激素的变化[J].生物工程学报.15(1):98-103.
徐春香,陈厚彬,李建国等. 2004.从香蕉胚性细胞悬浮系获得再生植株[J].植物生理学通讯.40(2):161-163.
徐华松,徐九龙,黄学林.1996.TDZ在植物组织培养中的作用[J].广西植物.16(1):77-80.
许亦农,麻密主译.2005.植物生物技术导论[M].化学工业出版社.
许煜泉,史益敏.1992.番茄离体子叶培养的形态发生及过氧化物酶的动态[J].上海农学院学报.10 (2):121-126.
许智宏,刘桂云.1980.烟草叶组织培养中愈伤组织和芽形成的细胞学观察[J].植物学报.22 (1):1-5.
杨和平,程井辰.1991.马唐胚性和非胚性愈伤组织生殖差异的初步研究[J].植物生理学通讯.27:337-340.
杨金玲,杜耀林,郭仲深.1999.自杄体细胞胚胎发生过程中过氧化物酶及酯酶同工酶的研究[J].武汉植物学研究.17(3):279-281.
杨玲.2007.花楸种子生物学和体细胞胚发生体系研究.东北林业大学博士学位论文.6.
杨昭庆,洪坤学,褚嘉佑.2000.单核苷酸多态性的研究进展[M].国外医学遗传学分册.23(1):4-8.
尹东,黄百渠,李彦舫.虎眼万年青的直接体细胞胚胎发生和植株再生[J].植物学报,1997,39(1):59-63.
由翠荣,曲复宁.2002.仙客来组织培养中不定芽形态发生的组织细胞学研究[J].烟台大学学报.15(4):273-279, 312.
袁彬鸿.2005.野牛草组织培养和再生植株体系的建立.中国农业大学硕士学位论文.5.
袁亚萍等.1997.常规玉米自交系及其杂交种幼胚培养反应的研究[J].吉林农业大学学报. 19(1):16-20.
曾寒冰.1993.小麦未熟胚离体培养的研究[J].东北农学院学报.20(3):205-210.
曾黎辉,吕柳新,王平等.2002.荔枝、龙眼胚性愈伤组织的细胞组织学观察[J].福建农林大学学报31(3):331-333.
张德华,张占甲,坤明等.1994.银杏组织培养研究.不同培养条件对银杏愈伤组织形成的影响[J].西北植物学报.6(1):29-32.
张静兰,唐定台,徐桂芳等.1982.防线素D和环已亚胺对激素诱导绿豆子叶脱分化及其核酸、蛋白质代谢的作用[J].植物学报.24:433-439.
张君成.1999.银杏叶愈伤组织增值过程中胜利生化特性的研究[J].三明师专学报.(3):21-26.
张剑侠等.2004.中国野生葡萄的离体培养与快速繁殖[J].园艺术学报.31(1):90-93.
张举仁.1987.亚麻下胚轴细胞脱分化过程的细胞学研究[J].山东大学学报.22(2):97-105.
张克荣,孙涛,李玉民等.1990.不同分化状态的白百利烟草愈伤组织在生长过程中核酸蛋白质的变化[J].武汉植物学研究.8(3):261-267.
张丕方,王崎.1989.烟草薄层培养中细胞早期动态的研究[J].植物学报.31:422-426.
张树录,郑国铝.1988.糜子组织培养中体细胞胚胎发生的扫描电镜观察[J].电子显微学报.7:1-4.
张彦妮.2005.复叶槭组织培养再生体系建立及其遗传转化初步研究.东北林业大学博士学位论文.6.
张志宏,景士西,王关林等.1997.苹果品种新乔纳金离体再生体系的建立[J].农业生物技术学报.5(3):305-306.
张志良,瞿伟菁主编. 2003.高等教育出版社[M].植物生理学实验指导.
张志扬,陈信波. 2006.亚麻生物技术研究进展[J].生物技术通讯.17(5):834-836.
张志扬. 2007.亚麻高效组织培养基遗传转化体系的建立.湖南农业大学硕士学位论文.6.
张自立,俞新大.1990.植物细胞和体细胞遗传学技术与原理[M].北京:高等教育出版社.
詹亚光.2001.白桦的组织培养和遗传转化的研究.东北林业大学博士学位论文.2.
赵洁,程井辰. 1992.石刁柏体细胞胚胎发生的细胞组织学研究[J].植物学报.34(7):491-495
郑文菊,王勋陵,贾敬芬.1999.小麦耐盐系愈伤组织细胞的超微结构观察[J].西北植物学报.19(1):81-84.
郑晓峰,黄百渠.1994.几种植物体细胞胚胎发生标记蛋白的研究[J].植物学报.36(3):175-180.
周俊彦,郭扶兴.1996.细胞分裂素类物质在植物体细胞胚发生中作用[J]植物生理学通讯.32(4):247-253.
牛爱国,牛海波等.1997.无籽西瓜组织快繁技术初步研究[J].山东农业科学.(10):32-34
甄艳,施季森.2007.林木体细胞胚发生分子生物学的研究进展[J].分子植物育种. (6):97-104
朱爱国译,喻春明校. 2002.亚麻[J].中国麻业.24(5):46-49.
朱长甫,镶田博,何弈昆等.1997.胡箩卜胚性细胞蛋白的分离研究[J].实验生物学报[J].30(1):13-18.
朱徽.1978.植物组织培养中的胚状体[J].遗传学报.5(1):79-87.
植物细胞与组织培养1978.中国科学院上海植物生理研究所.[M].上海科学技术出版社.
Allen R D, Dayton W, Craig L N.1984.Analysis of differential gene expression during somatic embryogenesis in opium poppy (Papaver somnirum L.)[J].Amer J Bot.71:120.
Ammirato PV.1983.Techniques for propagation and breeding. In: Evans DA,Sharp WR, Ammirato PV (eds). Handbook of Plant Cell Culture. Vol I[M]. NewYork: Macmillan Pub.82-84.
Ammirato PV. 1974.The effects of abscisic acid on the development of somatic embryos from cells of caraway(Carum carvi L. )[J]. Rot Gaz.135:328-337.
Ammirato PV.1983.The regulation of somatic embryos development in plant cell cultures: suspension culture techniques and hormone requirements[J]. Biotechnology.1:68-74.
Arnold Sara Von.1979.Induction and development of adventitious buds bud Prinordia On embryos buds and needles of Norway spruce (Picea abies (L.) Karst.)grown In Vitro[J]. Institute of Physiologicl Botany University of Uppsala,Sweden.5 (11):1-32.
Bakarat MN , Cocking EC.1983.Plant regeneration from protoplastderived tissues of Linum usitatissimum L. (Flax) [J]. Plant Cell.Rep. 2:314-317.
B. Bhagwat &W. David Lane.2004.In Vitro Shoot Regeneration from Leaves of Sweet Cherry(Prunus avium) Lapins and Sweetheart[J].Plant Cell, Tissue and Organ Culture 78:173-181.
Briggs BA, Mc Culloch SM, Edick LA.1988.Micropropagation of zaleas using Thidazuron [J].Acta Hort.226:205-208.
Briggs BA, Mc Culloch SM, Edick LA.1988.Micropropagation of Azaleas using Thidazuron [J].Acta Hort.227:330-333.
Botsein DR, White RL, Skolnick M, et al. 1980.Construction of genetic linkage map in man using restriction fragment length polymorphism[J].AM.J.Hum,Genet.32:314-331.
Brown DCW,Finstad KI,Watson EM.1995.Somatic embryogenesis in herbaceous dicots. In:Thorpe TA(ed)In vitro embryogenesis in plants[J].Kluwer,Dordrecht,PP 345-415.
Caiheiros MBP, Vieira LGE, Fuemes SRL. 1994. Effects of exogenous polyamines on directsomatic embryogenesis in coffee[J]. Rivista Brasileira de Fisiologia Vegetal .6(2):109-114.
Campbell, J.D. Procunier, B.D. Oomah, et al. 1995.Analysis of genetic relationships in flax (Linum usitatissimum) using RAPD markers[J]. Flax Inst. U.S. Proc.56:177-181.
Carimi F, De Pasquale F, Crescimanno FG.1999.Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus[J].Plant Cell Rep.18(10):935-940.
Carter, J.F. 1993.Potential of flaxseed and flaxseed oil in baked goods and other products in human nutrition[J]. Cereal Foods World.38:753-759.
Chen L J, Lutheds. 1987. Analysis of protein from embryonic and nonembryonie rice (Oryza saliva L.)[J].Plant Science.48:181-188.
Choi J H et al. 1987.Cloning of genes developmentally regulated during plant embryogenesis[J]. Proc. Natl. Acad. Sci. USA.84:1906-1910.
Chu,C.C.1978.The N6 medium and its applications to anther culture of cereal crops[J].In:Proc.Symo.Plant Tissue Culture,Science Press,Peking,pp.43-45. Clark A J. 1991.An embryo-specific protein of barley[J]. Eur J Biochem.199(1):115-121.
Creasy L L.1968.The increase in phenvlalanine a mmonia- lyase activity in Strawberry leaf disks and It's correlation with f1avord synthesis[J]. Phytorchenmistry.7:441-446.
Cunha AC,Fernandes-Ferreira M.1999.Influence of medium parameters on somatic embryogenesis from hypocotyl explants of flax (Linum usitatissimum L.) Effect of carbon source, total inorganic nitrogen and balance between ionic forms and interaction between calcium and zeatin [J]. Plant Physiol. 155:591-597.
Cunha AC,Fernandes-Ferreira M. 2003.Ontogenic variations in free and esterified fatty acids during somatic embryogenesis of flax (Linum usitatissimum L.)[J]. Plant Sci.164:863-872.
Cunha ACG,Ferreira MF. 1996.Somatic embryogenesis, organogenesis and callus growth kinetics of flax [J]. Plan Cell Tiss. Org.47:1-8.
Cunha A,Ferreira MF. 1997.Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax[J].Plant Sci. 124:97-105.
David H, David A, Bade P.1994.Cell wall composition and morphogenic response in callusderived from protoplasts of fiber flax (Linum usitatissimum L.) genotypes [J]. Plant Physiol.143:379-384.
Das S, Jha T B.1996.Jhas. in vitro micropropagation of cashewnut[J]. Plant Cell Reports, 15: 615- 619.
Dantu PK., Bhojwani SS. 1987. In vitro Propagation and Corm Formation in Gladiolus[J]. Garten bauwissen schaften.52:90-93.
De Bruyn MH, Ferreira DI. 1978. In vitro Corm Production of Gladiolus Dalenii and G.tristis.Physiol[J]. Plant.43:13-18.
Dedicova B, Hricova A, Samaj J, et al. 2000.Shoots and embryo-like structures regenerated from cultured flax (Linum usitatissimum L.)hypocotyl segments.[J]. Plant Physiol.157:327-334.
Dillman, A.C. 1953.Classification of flax varieties[J]. USDA, Technical Bulletin.1064.
Dong J.Z. and Dunstan D.I. 1995.Molecular biology of somatic embryogenesis in conifers[J]. In Jain S.M.and Minocha S.C.(Eds), Molecular biology of Wood Plant, Vol.l.Kluwer Axademic Publishers,Dordrecht.pp:51-87.
Dunstan DI,Tautorus TE,Thorpe TA.1995.Somatic embryogenesis in woody plants[J]. In:Thorpe TA(ed) In vitro embryogenesis in plants .Kluwer,Dordrecht,pp471-538.
Dure I, Greenway SG, Galau GA. 1981.Developmental biochemistry cottonseed embryogenesis and germination: Changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis [J]. Biochemistry.20:4162-4168.
Escalettes V,Dosba F. 1993.In Vitro Adventitious Shoot egeneration from Leaves of Prunus spp [J]. Plant Sci. 90: 201-209.
Fernando SC, Gamage C. 2000.Abscisic acid induced somatic embryogenesis in immature embryo explauts [J]. Plant Sci, 151(2):193-198.
Fluminhan A,De Aguitar-Perecin MLR,Dos Santos JA. 1996. Evidence for heterochromatin involvementin chromosome breakage in maize callus culture[J].Ann Bot.78:73-82.
Franz P F, DE Ruijter N C A, Schenl JH N. 1989.Isozyme as biochemical and cytological markers in embryogenic callus cultures of maize(Zea mavs L.) [J].Plant Cell Rep.8:67-70.
Hellentiaris T, Slocum M, Wright S et al. 1986.Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphism[J].Theor. Genet. Appl. 72:761-769.
Gaj M.D. 2004.Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana (L.) Heynh[J]. P1ant Growth Regulation. 3:27-47.
Gamborg,O.L.,Miller,R.A.,and Ojima,K.1968.Nutrition requirements of suspension cultures of sobean root cells[J].Expt.Cell.Res.,50:151-158.
Gamborg OL ,Shyluk JP .1976.Tissue culture, protoplasts and morphogenesis in flax[J]. Bot. Gaz. 137:301-306.
Garg K, Green P, Nickerson D A. 1999.Identification of candidate coding region singlenucleotide polymorphisms in 165 human genes using assembled expressed sequence tags[J]. Genome Res. 9(11):1087-1092.
Giroux R W, Pauls K P. 1996.Characterization of embryogenesis-related protein in alfalfa (Medieago .rativa)[J]. Physiologic Plantarum.96(4):585-592.
Hammatt N,Grant NJ.1998.Shoot Regeneration from Leaves of Prunus serotina Ehrh.(Black Cherry) and P. avium L. (Wild Cherry) [J].Plant Cell Rep.17:526-530.
Hennosa M R, Grondona L, Diaz-Minguez J M et al. 2001.Development of a Strain-Specific SCAR Marker for the Detection of Trichoderma Atroviride,a Biological Control Agent Against Soilborne Fungal Plant Pathogens[J]. Curr.Genet.38,343-350.
Hita O., Lafarga C. and Guerra H. 1997.Somatic embryogenesis from chickpea (Cicer arietinum L.)immature cotyledons: the effect of zeatin, gibberelllic acid and indole-3-butyric acid[J]. Acta Physiol. Plant.19:333-338.
Huang B C.1989.Search of molecular marker for the somatic embryogenesis in Arabidopsis thaliana L[J]. Chinese J Bot.1:25-34.
Huetteman CA., Preece JE,.1993.Thidiazuron: A Potent Tissue Culture [J]Plant Cell Tissue Org. Cult. 33:Cytokinin for Woody Plant Cell 105-121.
Hutchinson M.J., Krishna Raj S. and Saxena P.K. 1997.Inhibitory effect of GA3 on the development of Thidiazuron-induced somatic embryogenesis in geranium (Pelargonium x hortorum Bailey)hypocotyls cultures[J]. Plant Cell Rep.16:435-438.
Hutchinson M.J., Krishna Raj S. and Saxena P.K. 1996. Morphoregulatory Role of Thidiazuron: Evidence of the Involvement of Endogenous Auxin in Thidiazuron-induced Somatic Embryogenesis in Geranium (Pelargonium hortorum Bailey) [J]. Plant Phys. 149: 573-579.
Hvoslefeide A K, Corke F M K. 1997.Embryogenesis specific protein changes in birch suspension cultures[J].Plant Celt Tissue and Organ Culture.51:35-41.
Jain SM, Gupta PK Newton RJ.1995.Somatic embryogenesis in woody plants[M]. Dordrecht: ,Kluwer Acdemic Publishers.17-143.
Jean-Pierre Peros, Laurent Torregrosa, Gilles Berger. 1998. Variability among Vitis vinifera cultivars in micropropagation, organogenesis and antibiotic sensitivity[J]. Journal of Experimental Botany.49(319):171-179.
Kamada H,Hiroshi Harada. 1987. Changes in the endogenous level and effects of ABA during somatic embryogenesis of Daucus carrot L [J]. Plants &.Cell Physiol.22(8):1423-1429.
Kintzios S, Manos C, Malcri O. 1999.Somatic embryogenesis from mature leaves of rose[J].Plant Cell Rep.18(5): 467-472.
Kolunow AM,Soltys K,Nito N,McClure S.1995.Anther,ovule seed,and nucellar embryo development in Citrus sinensis cv[J].Valencia. can J Bot 73:1567-1582.
Koveza O V, Kokaeva Z G, Gostimskii S A et al. 2001.Production of a SCAR marker in pea Pisum Sativum L. Uusing RAPD analysis [J]. Genetika.37,574-576.
Krishnaraj S,Vasil IK.1995.Somatic embryogenesis in herbaceous monocors[J]. In:Thorpe TA (ed) In vitro embryogenesis in planrs.Kluwer Dordrecht,pp417-470.
Laibach F.1925.Das Taubwerden von Bastardsamen and die Kunstliche aufzucht von fruh absterbenden Bastardembryonen[J]. Z. Bot. 17: 417-459.
Lakshmanan P, Taji A.2000.Somatic embryogenesis in leguminous plants[J]. Plant. Biol. 2:136-148.
Lane WD. 1998.Iketani H&Hayashi T. Shoot Regeneration from Cultured Leaves of Japanese Pear[J]. Plant Cell Tiss. Org.Cult.54:9-14.
Lay, C.L., J.J. Hammond. 1984.Highlights of flax breeding and genetics[J]. Flax Inst. U.S. Proc.50:8-11.
Ling HQ,Binding H.1992.Improvement of plant regeneration from Linum protoplasts by the induction of somatic embryogenesis [J]. Plant Physiol.139:422-426.
Ling HQ,Binding H.1987.Plant regeneration from protoplasts in Linum [J]. Plant Breed.98:312-317.
Ling HQ,Binding H. 1997.Transformation in protoplast cultures of Linum usitatissimum and L. suffruticosum mediated with PEG and with Agrobacterium tumefaciens [J].Plant. Physiol. 151:479-488.
Link GKK, Eggera V.1946.Mode, site and time of initiation of hypocotyledonary bud primordial in Linum usitatissimum L[J]. Bot. Gaz. 107: 441-454.
LiX,Wang RR,Larson S et al. 2001. Development of a STS marker assay for detecting loss of heterozygosity in rice hybrids[J].Genome.44(l):23-26.
Mante SR,Scorza R.,Cordis JM.1989.Plant Regeneration from Cotyledons of Prunes persica, Prunes domestica and Prunes cerasus[J].Plant Cell Tiss.Org.Cult., 19:1一11.
Markert CL,Moller F. 1959. Multiple forms of enzymes: tissue, ontogenetic, and species-specific patterns[J]. Proc. Natl. Acad. Sci. USA.45:753-763.
Marks T R,Simpson S E.1990.Reduced phenolic oxidation at culture initia-tion in vitro following the exposure of field-grown stock- plants to darkness or low level of irradialce[J]. Hort. Sci. 68:103-111.
Mckee GW.1973. Chemical and biochemical techniques for varietal identifications[J]. Seed Sci. Technol. 1:181-199.
Merkle SA, Battle PJ. 2000.Enhancememnt of embryogenic culture initiation from tissues of mature sweetgum trees[J]. Plant Cell Rep.19(3): 268-273.
Merkle S A , Parrott W A, Flinn B S. 1995.Morphogenic aspects of somatic embryogenesis. In: Thorp T.A, In Vitro Embryogenesis in Plants[M]. Kluwer Axademic Publishers, Dordrecht.pp:155-203.
McSheffrey SA, McHughen A & Devine MD 1992 Characterization of transgenic sulfonylurea-resistant flax (Linum usitatissimum). [J]Theor. Appl. Genet. 84: 480–486
Murashige T, Skoo F.1962.A revised medium for rapid growth and bioassays with tobacco tissue culturew[J].Physiol.Plant,15:473-497.
Murthy BNS,Murch SJ and Saxena PK.1995.Thidiazuroninduced Somatic Embryogenesis in Intact Seedlings of Peanut (Arachis hypogaea L.): Endogenous Growth Regulator Levels and Significance of Cotyledons[J]. Physiol Plant.94: 268-276.
Murray BE, Handyside RJ, KellerWA.1977.In vitro regeneration of shoots on stem explants in haploid and diploid flax (Linumusitatissimum) [J]. Can. J. Genet. Cytol.19:177-186.
Nichterlein K,Friedt W.1993.Plant regeneration from isolated microspores of linseed(Linum usitatissimum L.) [J]. Plant Cell Rep.12:426-430.
Nichterlein K,Umbach H,Friedt W. 1991.Genotypic and exogenous factors affecting shoot regeneration from anther callus of linseed (Linum usitatissimum L.) [J].Euhytica.58:157-164.
Paglia G P, Olivieri A M, Morsante M. 1998.Towards Second-Generation STS (Sequence -Tagged Sites) Linkage Maps in Conifers: a Genetic Map of Norway Spruce [J].Mol. Gen. Genet.258,466-478.
Pawlicki N,Welander M.1994. Adventitious Shoot Regeneration from Leaf Segments of in Vitro Cultured Shoots of the Apple Rootstock Jork [J].Hort. Sci. 69: 687-696.
Penner GA, Bush A, Wise R.1993.Reproducibility of randomly amplified polymorphic DNA (RAPD) analysis among laboratories[J]. PCR Methods Appl,2:241-244.
Pinto G, Santos C, Neves L, et al. 2002Somatic ernbryogenesis and plant regeneration in Eucalyptus globulus Labill[J]. Plant Cell Rep.21(3):208-213.
Raghavan V. 1976.Adventive embryogenesis: Induction of diploid embryoids. In: Raghavan V ed, Experimental Embryogenesis in Vascular Plants[M].Chapter 14. London: Academic Press.349-381.
Ramage C., Williams R.R. 2002.Mineral Nutrition and Plant Morphogenesis. In Vitro Cell[J]. Dev. Biol. Plant,38:116-124.
Regener F, Roman H. 1994. Regeneration of grapevine by means of organogenesis[J]. Mitteilungen Klosterneuburg, 44(5):168-174.
RohlF.J.NTSYS-PC2.1.1997.Numerical taxonomy and multivariate analysis system[J]. Exwter Software, Setauket,NY.
Rowland, G.G., and R. Wilen. 1998.New trends in linseed breeding. p32–35 In Proceedings of the bast fibrous plants today and tomorrow[J].Vavilov Research Institute of Plant Industry, St. Petersburg, Russia,11:28-30.
Rudus L, Kepcrynska E, Kepcrynska J. 2002.Regulation of Medicago sativa L. somatic embryogenesis by gibberellins[J]. Plant Growth Regulat.36:91-95.
Rutkows ka-KrauseI, Mankowska q Lukaszewicz M, Szopa J. 2003.Regeneration of flax (Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum [J]. Plant Cell Rep.22:110-116.
Stamp J A, Colby S M. 1990.Direct shoot organogenesis and plant regeneration from leaves of grape (vitis spp.)[J].Plant Cell, Tissue and Organ Culture.22(2):127-133.
Stamp J.A., Colby S.M.1990.Improved Shoot organagenesis from leaves of grape[J].Amer.Sco.Hor.Sci.115(6):1038-1042.
Sterk P, De Vries, S C. 1987.Molecular markers for plant embryos. In:K Redenbaugh Synseeds: Stirn S, Jacobsen H J. Marker proteins for embryogenic differentiation patterns in pea callus[J].Plant Cell Rep.6:50-54.
Steward FC, Mes MO, Mears K. 1958.Growth and organized development of cultured cells. II. Organization cultures grown from suspended cells[J].Amen F. Bot.45:705-708.
Street HE, Withers LA. 1974.The anatomy of embryogenesis in culture.in street H E ed., Tissue culture and Plant Science Proceedings 3rd International Congress of Plant Tissue and Cell Cuture[J]. London:Academic Press.71-100.
Tian LN, Brown GW. 2000.Improvemet of soybean somatic embryo development and maturation by abscisic acid treatmem.[J]Canada.Plant Sci, 80(2):271-276.
Tautz D. 1989.Hypervariabi1ity of simple sequences as a general source for polymorphic DNA markers[J]. NuclAcid Res. 17(16): 6463-6471.
Tang F A, Mullins M G..1990.Adventitious bud formation in leaf explant of some grapevine rootstock and scion cultivars[J]. Viits.29: 151-158.
Tang H R,REN I L,Reustle G ,et al. 2002.Plant Regeneration from Leaves of Sweet and Sour Cherry Cultivars[J]. Scientia Horticulturae.93:235-244.
Tejavathi DH, Sita GL,Sunita AT. 2000.Somatic embryogenesis in flax[J]. Plant Cell Tiss. Org.63:155-159.
Thorpe.T.A.and T.Murashige. 1970.Some histochemical changes underlying shoot initiation in tobacoo callus cultures[J]. Can, J. Bot.48:277-285.
Thorpe TA,Stasolla C.2001.Somatic embryogenesis .In:Bhojwani SS,Soh WY (eds) Current trends in the embryology of angiosperms[J].Kluwer Dordrcht,pp 279-336.
Tokuji Y, Kutiyama K. 2003.Improvement of gibberellins and cytokinin in the formation of embryogenic cell clumps in carrot (Daucus carota) [J]. Plant Physiol.160:133-141.
Torregrosa L, Bouquet A. 1996.Adventitous bud formation and shoot development from in vitro leaves of Vitis x Muscadinia hybrids[J]. Plant Cell, Tissue and Organ Culture.45:245-252.
Vallegos CE, Chase CD. 1991. Linkage between isozyme markers and a locus affecting seed size in Phaseolus vulgaris L[J]. Theor. Appl. Genet, 81:413-419.
Van Engelen FA, De Vries, S C. 1992. Extracellular protein in plant embryogenesis[J]. Trends Genet.8:66-70.
Van Nieuwkerk,JP., Zimmerman RH., Fordham I. 1986. Thidiazuron Stimulation of Apple Shoot Proliferation in Vitro[J]. HortScience, 21:516-518.
Velappam N, Sondgrass J L, Hakovirta J R. 2001.Rapid identification of pathogenic bacteria by single-enzyme amplified fragment length polymorphism analysis[J]. Diagnostic microbiology and infections disease.39:77-83.
Vendenhou T H, Swennen R, Demot R, et al. 1999. Protein changes associated with somatic embryogenesis in cotton (Gossypium hirsutem L.)[J]. Mededelinge Facuitei Land Bouwkundigeen Toegepaste Biolgische Wetenschap-pen Universiteit Gent. 64 (56):451-454.
Weber J, Diez J, Selosse MA et al. 2002. SCAR Markers to Detect Mycorrhizas of an American Laccaria Bicolor Strain Inoculated in European Douglas-Fir Plantations[J]. Mycorrhiza.12,19-27.
Welsh J, McClelland M.1990.Genome fingerprinting using PCR with arbitrary primers[J]. Nucleic Acids Res.8:7213-7218.
Williams J G..1990.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. Nucleic AcidsRes.18:6531-6535.
Yadav JS, Rajam MV. 1997.Spatial distribution of free and conjugaged polyamines in leaves of solamum melongena L. associated with differential morphogenetic capacity:efficient somatic embryogenesis with putrescine[J].J Exp Bot.48(313):1537-1545.
Yepes LM, Aldwinckle HS. 1994.Factors that Affect Leaf Regeneration Efficiency in Apple and Effect of Antibiotics in Morphogenesis[J]. Plant Cell Tis and Cult.37:257-269.
Yong-Bi Fu. 2000.Effectiveness of bulking procedures in measuring population-pairwise similarity with dominant and co-dominant genetic markers [J].Theor. Appl. Genet. 100: 1284-1289.
Yong-Bi Fu, Gordon G. Rowland, Scott D, et al. 2003.RAPD Analysis of 54 North American Flax Cultivars[J].Crops Sci.43:1510-1515.
Yong-Bi Fu. 2005.Geographic Patterns of RAPD Variation in Cultivated Flax[J].Crops Sci.45: 1084-1091.
Zaffari GR,Peres LEP, Kerbauy GB.1998.Endogenous levels of cytokinins, indoleaceticacid, abscisic acid,and pigments in variegated somaclones of micropropagated banana leaves [J].Plant Growth Regul.17 (2) :59-61.
陈春玲.2001.龙眼体细胞胚胎发生机理的初步研究.福建农林人学硕士学位论文.5.
陈菲.2005.植物组织培养的防褐化探讨[J].北方园艺(2)69-70.
陈婷,李力.2000.水稻悬浮体细胞胚胎发生及其特异蛋白[J].东北师大学报(自然科学版). 32(4):52-58.
陈晓慧.2003.苎麻体系胞胚胎发生与原生质体培养研究.华中农业大学硕士学位论文.
陈秀芳,工坤范.1995.桃果实发育中褐变因子变化规律的研究[J].园艺学报.22:230-234.
陈一峰,周燮,汤日圣等.1998.水稻体细胞培养中胚性细胞出现与IAA的关系植物学报[J]. 40(5):474-477.
崔福君.2003.酿酒葡萄组织培养及再生系统的建立.西北农林科技大学硕士研究生学位论文.
崔凯荣,陈克明,王晓哲.1993.植物体细胞胚发生研究的某些现状[J].植物学通报.10(1):14-20.
崔凯荣,王亚馥.1993.石刁柏组织培养中体细胞胚发生的组织学观察[J].西北植物学报.13:203-206.
崔凯荣,高金成,王亚馥等.1994.石刁柏组织培养中体细胞胚发生的超微结构的研究[J].兰州大学学报(自然科学版)30(增刊):60-65.
崔凯荣,裴新梧,秦琳等.1998.ABA对枸杞体细胞胚发生的调节作用[J].实验生物学报.31(2):195-201.
崔凯荣,戴若兰主编.2000.植物体细胞胚胎发生的分子生物学[M].科学出版社.3.17.
崔凯荣,刑更生,周功克等.2000.植物激素对体细胞胚发生的诱导和调节[J].遗传.22(4): 349-354.
崔凯荣,邢更生,周功克等.2001.体细胞胚发生的生化基础[J].生命科学.1:28-33.
戴均贵,周吉源.1997.华黄茂离体形态发生过程中生理生化特性变化的研究[J].华中师范大学学报:自然科学版.31(2):220-224.
戴若兰,张玮,邢更生等. 1999.拘祀体细胞胚胎发生中蛋白质代谢动态的立体计量C17[J].西北植物学报.19(2):266-269.
邓欣,陈信波,龙松华.2007.10个亚麻品种亲缘关系的RAPD分析[J].中国麻业学.29(4).
杜娟,常国权,季静. 2002.玉米自交系愈伤组织的诱导、RAPD分析及扫描电镜观察[J].作物学报.28(2):282-285.
范国强,黎明,贺窑青等.2004.悬铃木体细胞胚胎发生及植株再生[J].林业科学.40(1): 71-74.
范双喜.1993.植物组织培养中胚状体诱导的研究[J].北京农学院学报.8(2):142-146.
范志辉,孙洪涛等.1997.亚麻体细胞无性系的建立及其植株再生[J].中国麻作.19(1):17-18.
付杨,陈凤清,孙冬雪等.2005.大叶柴胡愈伤组织继代培养及其RAPD分析[J].东北师大学报自然科学版.137:2.
高莉萍,包满珠.2005.月季的植株再生及遗传转化研究进展[J].植物学通报.22(2):231-237.
高国训.1999.植物组织培养中的褐变问题[J].植物生理学通讯.35:501-506.
高述民,陆帼一,杜葱芳.2001.大蒜体细胞胚发育分化中特异蛋白和某些生理生化变化(简报)[J].植物生理学通讯.37(3):207-210.
桂进,桂耀林,郭仲探.1992.三七体细胞胚胎发生及人工种子研制[J].实验生物学报.25(2): 139-147.
郭翔宇.2002.黑龙江省亚麻业的优势与面临的挑战[J].中国麻业.(2):43-45.
郭仲探,陆文楔.1984.玉米花粉愈伤组织产生胚状体的细胞形态学研究[J].植物学报.26(1):19-23.
郭子彪,盖钧镒.1997.内源激素IAA,ABA对大豆萌发子叶胚性愈伤组织诱导及其分化的调控[J].大豆科学.16(3):194-198.
谷瑞升,蒋湘宁,郭仲深.1999.植物离体培养中器官发生调控机制的研究进展[J].植物学通报.16(3): 238-244.
顾玉红,高述民,郭惠红等.2004.文冠果的体细胞胚胎发生[J].植物生理学通讯.40 (3):311-313.
韩碧文,刘淑兰.1988.植物离体体细胞胚胎发生[J].植物生理学通讯.10(1):9-15.
韩碧文.1989.胚状体发生的细胞学和生理生化研究[J].植物学通报.6(1):1-4.
郝再彬,徐仲,苍晶,2002.植物生理实验技术[M].68-69.111-112,123-124,195-197.
郝建平,裴建文,周小梅等.1993.蛇床胚状体发生的扫描电镜观察[J].山西大学学报.16(3): 322-324.
贺红,潘瑞炽,韩美丽等.1998.红江橙体胚发生及影响因素的研究[J].广西植物.18(4): 343-346.
洪岚,叶万辉,沈浩等.2005.薇甘菊组织培养及体细胞胚胎发生的研究[J].浙江大学学报(农业与生命科学版).31(5):572-578.
黄璐,卫志明.1999.同基因型玉米的再生能力和胚性与非胚性愈伤组织DNA的差异[J].植物生理学报.25(4):332-338.
黄素华,赖钟雄.2006.荔枝体细胞胚性培养物的DNA提取方法和RAPD分析[J].热带作物学报.27(4).
黄学林.1995.高等植物离休组织培养的形态建成及其调控[M].北京:科学出版社30-45.
郏艳虹,熊庆娥.植物体细胞胚胎发生的研究[J].四川农业大学学报.2003, 21(3):263-266.
焦德志,李波等.2006.亚麻愈伤组织诱导最佳培养基的筛选[J].安徽农业科学,34(6):1124-1125.
冀艾青.2003.核桃体细胞胚胎发生影响因子的研究.陕西农业大学.硕士研究生学位论文.7.
季道藩主编.1984.普通遗传学[M].北京:中国农业出版社.
揭雨成,周青文,陈佩度.2002.苎麻栽培品种亲缘关系的RAPD分析[J].作物学报.2:254-259.
暨淑仪,严学成等.1995.茶叶片愈伤组织形成的细胞组织学观察.茶叶.21(2)11-13.
姬妍茹,田玉杰等.2001.用组织培养法繁殖野生亚麻的研究[J].中国麻作.23(4):8-11.
姬妍茹,田玉杰.2005.亚麻直接分化再生系统的初步建立[J].生物技术通报.3:41-46.
李代丽.2007.白刺愈伤组织培养中外源激素对内源激素影响的研究.北京林业大学硕士学位论文.6.
李宏潮.1990.小麦成熟胚培养及影响因子的研究.植物细胞工程与育种[M].北京工业大学出版社.308-313.
李军生,李春瑶.1990.甘蔗的植物组织培养[J].广西植物.10(1):75-79.
李文雄,曾寒冰.2006.小麦研究[M].黑龙江科学技术出版社.431-434.
凌定厚,D S Brar,FJ Zapata.1988.籼稻体细胞胚胎发生的组织学及细胞学研究[J].植物学报.30(5):485-489.
林汉章,鲁雪华,陈扬春.1987.非洲紫罗兰胚状体发生的扫描电镜观察[J].福建省农科院学报.2:79-80.
林士杰,姜静,冯昕.2001.黑林1号杨组培叶片不定根的发生与内、外源激素关系的研究[J].林业科技.3(1):8-11.
林娅,郑玉梅,刘青林.2006.影响月季愈伤组织诱导和分化的因素[J].分子植物育种.(6):223-227.
刘涤,迟静芬,刘桂芸.1986.烟草愈伤组织器官发生过程中外源激素的作用[J].植物生理与分子生物学学报.12(1):104-108.
刘华英,萧浪涛,鲁旭东等.2004.柑橘体细胞胚发生的组织细胞学研究[J].果树学报.21(4):311-314.
刘华英,萧浪涛,何长征.2002.植物体细胞胚发生与内源激素的关系研究进展[J].湖南农业大学学报.28(4): 350-354.
刘天磊,江晓雯,王仑山.2002.苜蓿组织培养中球形胚发生时特异蛋白质和同工酶分析[J].西北植物学报.22(3):625-628.
刘瑛,彭渊,赖小萍等,2003.利用组织培养法保存野生苎麻资源的初步研究[J].江西农业学报.15(1),29-32.
刘勇刚,徐子勤,郝建国.2001.温度和水分对不同基因型小麦未成熟胚体细胞胚胎发生以及分化能力的影响[J].西北植物学报.3(5):425-431.
李学宝,陈光荣等.1995.亚麻下胚轴离体培养和转化的研究[J].武汉植物学研究.13(4):344-348.
赖钟雄,陈春玲.2002.龙眼体细胞胚胎发生过程中的内源激素变化[J].热带作物学报,23(2):41-47.
陆敏,黄静.1990.棉枣胚状体发生的扫描电镜观察[J].电了显微学报.9(3):32.
鲁旭东.2004.石刁柏UC800体细胞胚发生系统的建立及其生理生化机理研究.湖南农业大学博士学位论文.11.
马新业.2003.泡桐体细胞胚胎发生及植株再生研究.河南农业大学硕士论文.6.
孟繁静,刘道宏,苏业瑜编著.1992.植物生理生化[M].中国农业大学出版社.259,278.
明凤,李文雄.2000.小麦胚性愈伤组织发生影响因素的研究[J].东北农业大学学报.31(1):1-6.
牛遇达.2007.北五味子体细胞胚发生的研究.东北林业大学硕士学位论文.6.
欧阳学智,谢绍萍,陈睦传等.1997.甜菊愈伤组织分生区细胞中膜内含物的超微结构研究[J].西北植物学报.17(3):326-331.
祁建民,周东新,吴为人等.2004.RAPD和ISSR标记检测黄麻属遗传多样性的比较研究[J].中国农业科学.37(12):2006-2011.
齐力旺,韩一凡,李玲等.2000.华北落叶松体细胞胚发生及遗传转化实验系统的建立[J].实验生物学报.33(4):357-365.
曲春香,奚惕,黄连山.1990.金丝桃的组织培养及快速繁殖[J].植物生理学通讯.(2):40-45.
阙国宁,郭达初,李金田译.1988.树木组织培养[M].北京,中国林业出版社.175,141-143.
屈妹存,刘选明.1998.百合鳞片细胞形态发生中生理生化特性研究[J].生命科学研究.2(4):288-294.
师校欣,杜国强,高仪等.1999.苹果离体叶片高效再生不定芽技术研究[J].果树科学.16(4): 255-258.
宋春凤等.1998.白芷愈伤组织的电镜制样技术及超微结构观察[J].河北师范大学学报.22(3):396-398.
宋仁美. 1985.体细胞胚胎发生.的生化和遗传学研究[J].国外遗传育种.1:31-32.
汤国庆,李文安.1995.在离体条件下拐芹形成胚性愈伤组织和非胚性愈伤组织过程中几种内源激素的变化[J].实验生物学报.28(2):203-208.
王彩云,白吉刚. 2001.桂花的组织培养.北京林业大学学报[J]23(1): 24-25
王关林.方宏筠. 2002.植物基因工程原理与技术[M].科学出版社.742-743.
王家福,刘月学.2000.批把胚性愈伤组织的诱导和保存[J].福建农业大学学报, 29(3):305-310.
汪丽虹,崔凯荣,白志根等.1996.石刁柏非胚性细胞和胚性细胞的超微结构及ATP酶的定位[J].兰州大学学报(自然科学版).32(4):123-127.
汪丽虹,王星,崔凯荣等.1996.石刁柏及党参体细胞胚胎发生中的淀粉代谢动态[J].植物学通报.13(1):41-45.
王洪隆,何俊英,马洪仁远等.1991.大蒜发芽叶愈伤组织细胞超微结构观察与过氧化物酶同工酶的变化[J].华北农学报.6(1):74-80.
王慧梅.2004.喜树组织培养与遗传转化的研究.东北林业大学博士学位论文.6.
王吉之,李克勤.1993.槐树子叶组织培养中的形态发生研究[J].实验生物学报.26(1): 39-49.
王凯基.1979.油橄榄(Dlea,europnea L)组织培养的细胞组织学研究.愈伤组织的建成[J].植物学报,21,(2):127-134.
王丽,鲍晓明,黄百渠等.1998.香雪兰外植体形态学极性决定的体细胞胚胎发生[J].植物学报. 40(2):138-143.
王亚馥,崔凯荣,汪丽虹.1994.小麦体细胞胚胎发生的超微结构研究[J].植物学报.36(6):418-422.
王亚馥,王仑山.1989.枸杞组织培养中形态发生的细胞组织学观察[J].兰州大学学报.25 (4):88-92.
王玉富.2005.我国亚麻生物技术的研究现状及发展[J].中国麻业.27(2):60-65. 王中仁.1996.植物等位酶分析[M].北京科学出版社.10-11.
魏日凤.2006.蔗糖与麦芽糖质量浓度及添加成分对水稻成熟胚培养的效应[J].福建农林大学学报.35(6):565-565.
吴殿星,胡繁荣主编.2004.植物组织培养[M].上海交通大学出版社.
吴绛云. 1981.苹果“黄太平”花药培养获得单倍体植株[J].科学通报.(6)21:1344.
吴永宏,张德颐,王丽蓉. 1993.植物细胞的同步培养技术.植物生理学通讯[J].29(2):108-110
肖关丽,杨清辉,李富生等.2002.甘蔗愈伤组织分化绿苗内源激素变化规律研究[J].西南农业大学学报.24(4):337-338.
肖显华,王庆华,林荣双等.1999.花生体细胞胚胚性特异蛋白的研究.[J].山东烟台大学学报.12(3):185-188.
肖显华,王顺珍,林荣双等.1998.花生成熟胚叶体细胞胚形成过程中的过氧化物酶同工酶研究[J].烟台大学学报.11(1):57-61.
谢海燕,毛碧增,单兰兰等.2004.狗牙根颖果胚性愈伤组织的诱导和胚性细胞的超微结构及植株再生[J].植物生理与分子生物学学报.30(2):209-215.
邢朝斌,沈海龙,刘岩等.2006.刺五加成熟种子的愈伤组织诱导及其RAPD分析[J].种子.25(7):44-49.
邢登辉,赵云云,黄承芬.1999.皇冠草体细胞胚发生及其体胚发生过程中内源激素的变化[J].生物工程学报.15(1):98-103.
徐春香,陈厚彬,李建国等. 2004.从香蕉胚性细胞悬浮系获得再生植株[J].植物生理学通讯.40(2):161-163.
徐华松,徐九龙,黄学林.1996.TDZ在植物组织培养中的作用[J].广西植物.16(1):77-80.
许亦农,麻密主译.2005.植物生物技术导论[M].化学工业出版社.
许煜泉,史益敏.1992.番茄离体子叶培养的形态发生及过氧化物酶的动态[J].上海农学院学报.10 (2):121-126.
许智宏,刘桂云.1980.烟草叶组织培养中愈伤组织和芽形成的细胞学观察[J].植物学报.22 (1):1-5.
杨和平,程井辰.1991.马唐胚性和非胚性愈伤组织生殖差异的初步研究[J].植物生理学通讯.27:337-340.
杨金玲,杜耀林,郭仲深.1999.自杄体细胞胚胎发生过程中过氧化物酶及酯酶同工酶的研究[J].武汉植物学研究.17(3):279-281.
杨玲.2007.花楸种子生物学和体细胞胚发生体系研究.东北林业大学博士学位论文.6.
杨昭庆,洪坤学,褚嘉佑.2000.单核苷酸多态性的研究进展[M].国外医学遗传学分册.23(1):4-8.
尹东,黄百渠,李彦舫.虎眼万年青的直接体细胞胚胎发生和植株再生[J].植物学报,1997,39(1):59-63.
由翠荣,曲复宁.2002.仙客来组织培养中不定芽形态发生的组织细胞学研究[J].烟台大学学报.15(4):273-279, 312.
袁彬鸿.2005.野牛草组织培养和再生植株体系的建立.中国农业大学硕士学位论文.5.
袁亚萍等.1997.常规玉米自交系及其杂交种幼胚培养反应的研究[J].吉林农业大学学报. 19(1):16-20.
曾寒冰.1993.小麦未熟胚离体培养的研究[J].东北农学院学报.20(3):205-210.
曾黎辉,吕柳新,王平等.2002.荔枝、龙眼胚性愈伤组织的细胞组织学观察[J].福建农林大学学报31(3):331-333.
张德华,张占甲,坤明等.1994.银杏组织培养研究.不同培养条件对银杏愈伤组织形成的影响[J].西北植物学报.6(1):29-32.
张静兰,唐定台,徐桂芳等.1982.防线素D和环已亚胺对激素诱导绿豆子叶脱分化及其核酸、蛋白质代谢的作用[J].植物学报.24:433-439.
张君成.1999.银杏叶愈伤组织增值过程中胜利生化特性的研究[J].三明师专学报.(3):21-26.
张剑侠等.2004.中国野生葡萄的离体培养与快速繁殖[J].园艺术学报.31(1):90-93.
张举仁.1987.亚麻下胚轴细胞脱分化过程的细胞学研究[J].山东大学学报.22(2):97-105.
张克荣,孙涛,李玉民等.1990.不同分化状态的白百利烟草愈伤组织在生长过程中核酸蛋白质的变化[J].武汉植物学研究.8(3):261-267.
张丕方,王崎.1989.烟草薄层培养中细胞早期动态的研究[J].植物学报.31:422-426.
张树录,郑国铝.1988.糜子组织培养中体细胞胚胎发生的扫描电镜观察[J].电子显微学报.7:1-4.
张彦妮.2005.复叶槭组织培养再生体系建立及其遗传转化初步研究.东北林业大学博士学位论文.6.
张志宏,景士西,王关林等.1997.苹果品种新乔纳金离体再生体系的建立[J].农业生物技术学报.5(3):305-306.
张志良,瞿伟菁主编. 2003.高等教育出版社[M].植物生理学实验指导.
张志扬,陈信波. 2006.亚麻生物技术研究进展[J].生物技术通讯.17(5):834-836.
张志扬. 2007.亚麻高效组织培养基遗传转化体系的建立.湖南农业大学硕士学位论文.6.
张自立,俞新大.1990.植物细胞和体细胞遗传学技术与原理[M].北京:高等教育出版社.
詹亚光.2001.白桦的组织培养和遗传转化的研究.东北林业大学博士学位论文.2.
赵洁,程井辰. 1992.石刁柏体细胞胚胎发生的细胞组织学研究[J].植物学报.34(7):491-495
郑文菊,王勋陵,贾敬芬.1999.小麦耐盐系愈伤组织细胞的超微结构观察[J].西北植物学报.19(1):81-84.
郑晓峰,黄百渠.1994.几种植物体细胞胚胎发生标记蛋白的研究[J].植物学报.36(3):175-180.
周俊彦,郭扶兴.1996.细胞分裂素类物质在植物体细胞胚发生中作用[J]植物生理学通讯.32(4):247-253.
牛爱国,牛海波等.1997.无籽西瓜组织快繁技术初步研究[J].山东农业科学.(10):32-34
甄艳,施季森.2007.林木体细胞胚发生分子生物学的研究进展[J].分子植物育种. (6):97-104
朱爱国译,喻春明校. 2002.亚麻[J].中国麻业.24(5):46-49.
朱长甫,镶田博,何弈昆等.1997.胡箩卜胚性细胞蛋白的分离研究[J].实验生物学报[J].30(1):13-18.
朱徽.1978.植物组织培养中的胚状体[J].遗传学报.5(1):79-87.
植物细胞与组织培养1978.中国科学院上海植物生理研究所.[M].上海科学技术出版社.
Allen R D, Dayton W, Craig L N.1984.Analysis of differential gene expression during somatic embryogenesis in opium poppy (Papaver somnirum L.)[J].Amer J Bot.71:120.
Ammirato PV.1983.Techniques for propagation and breeding. In: Evans DA,Sharp WR, Ammirato PV (eds). Handbook of Plant Cell Culture. Vol I[M]. NewYork: Macmillan Pub.82-84.
Ammirato PV. 1974.The effects of abscisic acid on the development of somatic embryos from cells of caraway(Carum carvi L. )[J]. Rot Gaz.135:328-337.
Ammirato PV.1983.The regulation of somatic embryos development in plant cell cultures: suspension culture techniques and hormone requirements[J]. Biotechnology.1:68-74.
Arnold Sara Von.1979.Induction and development of adventitious buds bud Prinordia On embryos buds and needles of Norway spruce (Picea abies (L.) Karst.)grown In Vitro[J]. Institute of Physiologicl Botany University of Uppsala,Sweden.5 (11):1-32.
Bakarat MN , Cocking EC.1983.Plant regeneration from protoplastderived tissues of Linum usitatissimum L. (Flax) [J]. Plant Cell.Rep. 2:314-317.
B. Bhagwat &W. David Lane.2004.In Vitro Shoot Regeneration from Leaves of Sweet Cherry(Prunus avium) Lapins and Sweetheart[J].Plant Cell, Tissue and Organ Culture 78:173-181.
Briggs BA, Mc Culloch SM, Edick LA.1988.Micropropagation of zaleas using Thidazuron [J].Acta Hort.226:205-208.
Briggs BA, Mc Culloch SM, Edick LA.1988.Micropropagation of Azaleas using Thidazuron [J].Acta Hort.227:330-333.
Botsein DR, White RL, Skolnick M, et al. 1980.Construction of genetic linkage map in man using restriction fragment length polymorphism[J].AM.J.Hum,Genet.32:314-331.
Brown DCW,Finstad KI,Watson EM.1995.Somatic embryogenesis in herbaceous dicots. In:Thorpe TA(ed)In vitro embryogenesis in plants[J].Kluwer,Dordrecht,PP 345-415.
Caiheiros MBP, Vieira LGE, Fuemes SRL. 1994. Effects of exogenous polyamines on directsomatic embryogenesis in coffee[J]. Rivista Brasileira de Fisiologia Vegetal .6(2):109-114.
Campbell, J.D. Procunier, B.D. Oomah, et al. 1995.Analysis of genetic relationships in flax (Linum usitatissimum) using RAPD markers[J]. Flax Inst. U.S. Proc.56:177-181.
Carimi F, De Pasquale F, Crescimanno FG.1999.Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus[J].Plant Cell Rep.18(10):935-940.
Carter, J.F. 1993.Potential of flaxseed and flaxseed oil in baked goods and other products in human nutrition[J]. Cereal Foods World.38:753-759.
Chen L J, Lutheds. 1987. Analysis of protein from embryonic and nonembryonie rice (Oryza saliva L.)[J].Plant Science.48:181-188.
Choi J H et al. 1987.Cloning of genes developmentally regulated during plant embryogenesis[J]. Proc. Natl. Acad. Sci. USA.84:1906-1910.
Chu,C.C.1978.The N6 medium and its applications to anther culture of cereal crops[J].In:Proc.Symo.Plant Tissue Culture,Science Press,Peking,pp.43-45. Clark A J. 1991.An embryo-specific protein of barley[J]. Eur J Biochem.199(1):115-121.
Creasy L L.1968.The increase in phenvlalanine a mmonia- lyase activity in Strawberry leaf disks and It's correlation with f1avord synthesis[J]. Phytorchenmistry.7:441-446.
Cunha AC,Fernandes-Ferreira M.1999.Influence of medium parameters on somatic embryogenesis from hypocotyl explants of flax (Linum usitatissimum L.) Effect of carbon source, total inorganic nitrogen and balance between ionic forms and interaction between calcium and zeatin [J]. Plant Physiol. 155:591-597.
Cunha AC,Fernandes-Ferreira M. 2003.Ontogenic variations in free and esterified fatty acids during somatic embryogenesis of flax (Linum usitatissimum L.)[J]. Plant Sci.164:863-872.
Cunha ACG,Ferreira MF. 1996.Somatic embryogenesis, organogenesis and callus growth kinetics of flax [J]. Plan Cell Tiss. Org.47:1-8.
Cunha A,Ferreira MF. 1997.Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax[J].Plant Sci. 124:97-105.
David H, David A, Bade P.1994.Cell wall composition and morphogenic response in callusderived from protoplasts of fiber flax (Linum usitatissimum L.) genotypes [J]. Plant Physiol.143:379-384.
Das S, Jha T B.1996.Jhas. in vitro micropropagation of cashewnut[J]. Plant Cell Reports, 15: 615- 619.
Dantu PK., Bhojwani SS. 1987. In vitro Propagation and Corm Formation in Gladiolus[J]. Garten bauwissen schaften.52:90-93.
De Bruyn MH, Ferreira DI. 1978. In vitro Corm Production of Gladiolus Dalenii and G.tristis.Physiol[J]. Plant.43:13-18.
Dedicova B, Hricova A, Samaj J, et al. 2000.Shoots and embryo-like structures regenerated from cultured flax (Linum usitatissimum L.)hypocotyl segments.[J]. Plant Physiol.157:327-334.
Dillman, A.C. 1953.Classification of flax varieties[J]. USDA, Technical Bulletin.1064.
Dong J.Z. and Dunstan D.I. 1995.Molecular biology of somatic embryogenesis in conifers[J]. In Jain S.M.and Minocha S.C.(Eds), Molecular biology of Wood Plant, Vol.l.Kluwer Axademic Publishers,Dordrecht.pp:51-87.
Dunstan DI,Tautorus TE,Thorpe TA.1995.Somatic embryogenesis in woody plants[J]. In:Thorpe TA(ed) In vitro embryogenesis in plants .Kluwer,Dordrecht,pp471-538.
Dure I, Greenway SG, Galau GA. 1981.Developmental biochemistry cottonseed embryogenesis and germination: Changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis [J]. Biochemistry.20:4162-4168.
Escalettes V,Dosba F. 1993.In Vitro Adventitious Shoot egeneration from Leaves of Prunus spp [J]. Plant Sci. 90: 201-209.
Fernando SC, Gamage C. 2000.Abscisic acid induced somatic embryogenesis in immature embryo explauts [J]. Plant Sci, 151(2):193-198.
Fluminhan A,De Aguitar-Perecin MLR,Dos Santos JA. 1996. Evidence for heterochromatin involvementin chromosome breakage in maize callus culture[J].Ann Bot.78:73-82.
Franz P F, DE Ruijter N C A, Schenl JH N. 1989.Isozyme as biochemical and cytological markers in embryogenic callus cultures of maize(Zea mavs L.) [J].Plant Cell Rep.8:67-70.
Hellentiaris T, Slocum M, Wright S et al. 1986.Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphism[J].Theor. Genet. Appl. 72:761-769.
Gaj M.D. 2004.Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana (L.) Heynh[J]. P1ant Growth Regulation. 3:27-47.
Gamborg,O.L.,Miller,R.A.,and Ojima,K.1968.Nutrition requirements of suspension cultures of sobean root cells[J].Expt.Cell.Res.,50:151-158.
Gamborg OL ,Shyluk JP .1976.Tissue culture, protoplasts and morphogenesis in flax[J]. Bot. Gaz. 137:301-306.
Garg K, Green P, Nickerson D A. 1999.Identification of candidate coding region singlenucleotide polymorphisms in 165 human genes using assembled expressed sequence tags[J]. Genome Res. 9(11):1087-1092.
Giroux R W, Pauls K P. 1996.Characterization of embryogenesis-related protein in alfalfa (Medieago .rativa)[J]. Physiologic Plantarum.96(4):585-592.
Hammatt N,Grant NJ.1998.Shoot Regeneration from Leaves of Prunus serotina Ehrh.(Black Cherry) and P. avium L. (Wild Cherry) [J].Plant Cell Rep.17:526-530.
Hennosa M R, Grondona L, Diaz-Minguez J M et al. 2001.Development of a Strain-Specific SCAR Marker for the Detection of Trichoderma Atroviride,a Biological Control Agent Against Soilborne Fungal Plant Pathogens[J]. Curr.Genet.38,343-350.
Hita O., Lafarga C. and Guerra H. 1997.Somatic embryogenesis from chickpea (Cicer arietinum L.)immature cotyledons: the effect of zeatin, gibberelllic acid and indole-3-butyric acid[J]. Acta Physiol. Plant.19:333-338.
Huang B C.1989.Search of molecular marker for the somatic embryogenesis in Arabidopsis thaliana L[J]. Chinese J Bot.1:25-34.
Huetteman CA., Preece JE,.1993.Thidiazuron: A Potent Tissue Culture [J]Plant Cell Tissue Org. Cult. 33:Cytokinin for Woody Plant Cell 105-121.
Hutchinson M.J., Krishna Raj S. and Saxena P.K. 1997.Inhibitory effect of GA3 on the development of Thidiazuron-induced somatic embryogenesis in geranium (Pelargonium x hortorum Bailey)hypocotyls cultures[J]. Plant Cell Rep.16:435-438.
Hutchinson M.J., Krishna Raj S. and Saxena P.K. 1996. Morphoregulatory Role of Thidiazuron: Evidence of the Involvement of Endogenous Auxin in Thidiazuron-induced Somatic Embryogenesis in Geranium (Pelargonium hortorum Bailey) [J]. Plant Phys. 149: 573-579.
Hvoslefeide A K, Corke F M K. 1997.Embryogenesis specific protein changes in birch suspension cultures[J].Plant Celt Tissue and Organ Culture.51:35-41.
Jain SM, Gupta PK Newton RJ.1995.Somatic embryogenesis in woody plants[M]. Dordrecht: ,Kluwer Acdemic Publishers.17-143.
Jean-Pierre Peros, Laurent Torregrosa, Gilles Berger. 1998. Variability among Vitis vinifera cultivars in micropropagation, organogenesis and antibiotic sensitivity[J]. Journal of Experimental Botany.49(319):171-179.
Kamada H,Hiroshi Harada. 1987. Changes in the endogenous level and effects of ABA during somatic embryogenesis of Daucus carrot L [J]. Plants &.Cell Physiol.22(8):1423-1429.
Kintzios S, Manos C, Malcri O. 1999.Somatic embryogenesis from mature leaves of rose[J].Plant Cell Rep.18(5): 467-472.
Kolunow AM,Soltys K,Nito N,McClure S.1995.Anther,ovule seed,and nucellar embryo development in Citrus sinensis cv[J].Valencia. can J Bot 73:1567-1582.
Koveza O V, Kokaeva Z G, Gostimskii S A et al. 2001.Production of a SCAR marker in pea Pisum Sativum L. Uusing RAPD analysis [J]. Genetika.37,574-576.
Krishnaraj S,Vasil IK.1995.Somatic embryogenesis in herbaceous monocors[J]. In:Thorpe TA (ed) In vitro embryogenesis in planrs.Kluwer Dordrecht,pp417-470.
Laibach F.1925.Das Taubwerden von Bastardsamen and die Kunstliche aufzucht von fruh absterbenden Bastardembryonen[J]. Z. Bot. 17: 417-459.
Lakshmanan P, Taji A.2000.Somatic embryogenesis in leguminous plants[J]. Plant. Biol. 2:136-148.
Lane WD. 1998.Iketani H&Hayashi T. Shoot Regeneration from Cultured Leaves of Japanese Pear[J]. Plant Cell Tiss. Org.Cult.54:9-14.
Lay, C.L., J.J. Hammond. 1984.Highlights of flax breeding and genetics[J]. Flax Inst. U.S. Proc.50:8-11.
Ling HQ,Binding H.1992.Improvement of plant regeneration from Linum protoplasts by the induction of somatic embryogenesis [J]. Plant Physiol.139:422-426.
Ling HQ,Binding H.1987.Plant regeneration from protoplasts in Linum [J]. Plant Breed.98:312-317.
Ling HQ,Binding H. 1997.Transformation in protoplast cultures of Linum usitatissimum and L. suffruticosum mediated with PEG and with Agrobacterium tumefaciens [J].Plant. Physiol. 151:479-488.
Link GKK, Eggera V.1946.Mode, site and time of initiation of hypocotyledonary bud primordial in Linum usitatissimum L[J]. Bot. Gaz. 107: 441-454.
LiX,Wang RR,Larson S et al. 2001. Development of a STS marker assay for detecting loss of heterozygosity in rice hybrids[J].Genome.44(l):23-26.
Mante SR,Scorza R.,Cordis JM.1989.Plant Regeneration from Cotyledons of Prunes persica, Prunes domestica and Prunes cerasus[J].Plant Cell Tiss.Org.Cult., 19:1一11.
Markert CL,Moller F. 1959. Multiple forms of enzymes: tissue, ontogenetic, and species-specific patterns[J]. Proc. Natl. Acad. Sci. USA.45:753-763.
Marks T R,Simpson S E.1990.Reduced phenolic oxidation at culture initia-tion in vitro following the exposure of field-grown stock- plants to darkness or low level of irradialce[J]. Hort. Sci. 68:103-111.
Mckee GW.1973. Chemical and biochemical techniques for varietal identifications[J]. Seed Sci. Technol. 1:181-199.
Merkle SA, Battle PJ. 2000.Enhancememnt of embryogenic culture initiation from tissues of mature sweetgum trees[J]. Plant Cell Rep.19(3): 268-273.
Merkle S A , Parrott W A, Flinn B S. 1995.Morphogenic aspects of somatic embryogenesis. In: Thorp T.A, In Vitro Embryogenesis in Plants[M]. Kluwer Axademic Publishers, Dordrecht.pp:155-203.
McSheffrey SA, McHughen A & Devine MD 1992 Characterization of transgenic sulfonylurea-resistant flax (Linum usitatissimum). [J]Theor. Appl. Genet. 84: 480–486
Murashige T, Skoo F.1962.A revised medium for rapid growth and bioassays with tobacco tissue culturew[J].Physiol.Plant,15:473-497.
Murthy BNS,Murch SJ and Saxena PK.1995.Thidiazuroninduced Somatic Embryogenesis in Intact Seedlings of Peanut (Arachis hypogaea L.): Endogenous Growth Regulator Levels and Significance of Cotyledons[J]. Physiol Plant.94: 268-276.
Murray BE, Handyside RJ, KellerWA.1977.In vitro regeneration of shoots on stem explants in haploid and diploid flax (Linumusitatissimum) [J]. Can. J. Genet. Cytol.19:177-186.
Nichterlein K,Friedt W.1993.Plant regeneration from isolated microspores of linseed(Linum usitatissimum L.) [J]. Plant Cell Rep.12:426-430.
Nichterlein K,Umbach H,Friedt W. 1991.Genotypic and exogenous factors affecting shoot regeneration from anther callus of linseed (Linum usitatissimum L.) [J].Euhytica.58:157-164.
Paglia G P, Olivieri A M, Morsante M. 1998.Towards Second-Generation STS (Sequence -Tagged Sites) Linkage Maps in Conifers: a Genetic Map of Norway Spruce [J].Mol. Gen. Genet.258,466-478.
Pawlicki N,Welander M.1994. Adventitious Shoot Regeneration from Leaf Segments of in Vitro Cultured Shoots of the Apple Rootstock Jork [J].Hort. Sci. 69: 687-696.
Penner GA, Bush A, Wise R.1993.Reproducibility of randomly amplified polymorphic DNA (RAPD) analysis among laboratories[J]. PCR Methods Appl,2:241-244.
Pinto G, Santos C, Neves L, et al. 2002Somatic ernbryogenesis and plant regeneration in Eucalyptus globulus Labill[J]. Plant Cell Rep.21(3):208-213.
Raghavan V. 1976.Adventive embryogenesis: Induction of diploid embryoids. In: Raghavan V ed, Experimental Embryogenesis in Vascular Plants[M].Chapter 14. London: Academic Press.349-381.
Ramage C., Williams R.R. 2002.Mineral Nutrition and Plant Morphogenesis. In Vitro Cell[J]. Dev. Biol. Plant,38:116-124.
Regener F, Roman H. 1994. Regeneration of grapevine by means of organogenesis[J]. Mitteilungen Klosterneuburg, 44(5):168-174.
RohlF.J.NTSYS-PC2.1.1997.Numerical taxonomy and multivariate analysis system[J]. Exwter Software, Setauket,NY.
Rowland, G.G., and R. Wilen. 1998.New trends in linseed breeding. p32–35 In Proceedings of the bast fibrous plants today and tomorrow[J].Vavilov Research Institute of Plant Industry, St. Petersburg, Russia,11:28-30.
Rudus L, Kepcrynska E, Kepcrynska J. 2002.Regulation of Medicago sativa L. somatic embryogenesis by gibberellins[J]. Plant Growth Regulat.36:91-95.
Rutkows ka-KrauseI, Mankowska q Lukaszewicz M, Szopa J. 2003.Regeneration of flax (Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum [J]. Plant Cell Rep.22:110-116.
Stamp J A, Colby S M. 1990.Direct shoot organogenesis and plant regeneration from leaves of grape (vitis spp.)[J].Plant Cell, Tissue and Organ Culture.22(2):127-133.
Stamp J.A., Colby S.M.1990.Improved Shoot organagenesis from leaves of grape[J].Amer.Sco.Hor.Sci.115(6):1038-1042.
Sterk P, De Vries, S C. 1987.Molecular markers for plant embryos. In:K Redenbaugh Synseeds: Stirn S, Jacobsen H J. Marker proteins for embryogenic differentiation patterns in pea callus[J].Plant Cell Rep.6:50-54.
Steward FC, Mes MO, Mears K. 1958.Growth and organized development of cultured cells. II. Organization cultures grown from suspended cells[J].Amen F. Bot.45:705-708.
Street HE, Withers LA. 1974.The anatomy of embryogenesis in culture.in street H E ed., Tissue culture and Plant Science Proceedings 3rd International Congress of Plant Tissue and Cell Cuture[J]. London:Academic Press.71-100.
Tian LN, Brown GW. 2000.Improvemet of soybean somatic embryo development and maturation by abscisic acid treatmem.[J]Canada.Plant Sci, 80(2):271-276.
Tautz D. 1989.Hypervariabi1ity of simple sequences as a general source for polymorphic DNA markers[J]. NuclAcid Res. 17(16): 6463-6471.
Tang F A, Mullins M G..1990.Adventitious bud formation in leaf explant of some grapevine rootstock and scion cultivars[J]. Viits.29: 151-158.
Tang H R,REN I L,Reustle G ,et al. 2002.Plant Regeneration from Leaves of Sweet and Sour Cherry Cultivars[J]. Scientia Horticulturae.93:235-244.
Tejavathi DH, Sita GL,Sunita AT. 2000.Somatic embryogenesis in flax[J]. Plant Cell Tiss. Org.63:155-159.
Thorpe.T.A.and T.Murashige. 1970.Some histochemical changes underlying shoot initiation in tobacoo callus cultures[J]. Can, J. Bot.48:277-285.
Thorpe TA,Stasolla C.2001.Somatic embryogenesis .In:Bhojwani SS,Soh WY (eds) Current trends in the embryology of angiosperms[J].Kluwer Dordrcht,pp 279-336.
Tokuji Y, Kutiyama K. 2003.Improvement of gibberellins and cytokinin in the formation of embryogenic cell clumps in carrot (Daucus carota) [J]. Plant Physiol.160:133-141.
Torregrosa L, Bouquet A. 1996.Adventitous bud formation and shoot development from in vitro leaves of Vitis x Muscadinia hybrids[J]. Plant Cell, Tissue and Organ Culture.45:245-252.
Vallegos CE, Chase CD. 1991. Linkage between isozyme markers and a locus affecting seed size in Phaseolus vulgaris L[J]. Theor. Appl. Genet, 81:413-419.
Van Engelen FA, De Vries, S C. 1992. Extracellular protein in plant embryogenesis[J]. Trends Genet.8:66-70.
Van Nieuwkerk,JP., Zimmerman RH., Fordham I. 1986. Thidiazuron Stimulation of Apple Shoot Proliferation in Vitro[J]. HortScience, 21:516-518.
Velappam N, Sondgrass J L, Hakovirta J R. 2001.Rapid identification of pathogenic bacteria by single-enzyme amplified fragment length polymorphism analysis[J]. Diagnostic microbiology and infections disease.39:77-83.
Vendenhou T H, Swennen R, Demot R, et al. 1999. Protein changes associated with somatic embryogenesis in cotton (Gossypium hirsutem L.)[J]. Mededelinge Facuitei Land Bouwkundigeen Toegepaste Biolgische Wetenschap-pen Universiteit Gent. 64 (56):451-454.
Weber J, Diez J, Selosse MA et al. 2002. SCAR Markers to Detect Mycorrhizas of an American Laccaria Bicolor Strain Inoculated in European Douglas-Fir Plantations[J]. Mycorrhiza.12,19-27.
Welsh J, McClelland M.1990.Genome fingerprinting using PCR with arbitrary primers[J]. Nucleic Acids Res.8:7213-7218.
Williams J G..1990.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. Nucleic AcidsRes.18:6531-6535.
Yadav JS, Rajam MV. 1997.Spatial distribution of free and conjugaged polyamines in leaves of solamum melongena L. associated with differential morphogenetic capacity:efficient somatic embryogenesis with putrescine[J].J Exp Bot.48(313):1537-1545.
Yepes LM, Aldwinckle HS. 1994.Factors that Affect Leaf Regeneration Efficiency in Apple and Effect of Antibiotics in Morphogenesis[J]. Plant Cell Tis and Cult.37:257-269.
Yong-Bi Fu. 2000.Effectiveness of bulking procedures in measuring population-pairwise similarity with dominant and co-dominant genetic markers [J].Theor. Appl. Genet. 100: 1284-1289.
Yong-Bi Fu, Gordon G. Rowland, Scott D, et al. 2003.RAPD Analysis of 54 North American Flax Cultivars[J].Crops Sci.43:1510-1515.
Yong-Bi Fu. 2005.Geographic Patterns of RAPD Variation in Cultivated Flax[J].Crops Sci.45: 1084-1091.
Zaffari GR,Peres LEP, Kerbauy GB.1998.Endogenous levels of cytokinins, indoleaceticacid, abscisic acid,and pigments in variegated somaclones of micropropagated banana leaves [J].Plant Growth Regul.17 (2) :59-61.