氟吗啉（SYP-L190）对日本鹌鹑毒性试验的初步研究
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摘要
鸟类毒性试验是重要的环境生物毒性试验之一，它在我国日益受到重视，目前，急性毒性试验是我国农药登记必备试验，而亚急性、亚慢性试验尚无系统研究报道。杀菌剂氟吗啉是由沈阳化工研究院研究开发的具有自主知识产权的含氟新农药。为建立一套鸟类长期毒性试验方法，初步研究氟吗啉对日本鹌鹑的亚急性、亚慢性毒性效应，本课题在进行急性LD50试验的基础上，进一步进行了42天经口灌胃试验；在进行LC50试验的基础上,进一步进行了90天喂养毒性试验。
试验结果表明，氟吗啉作用于日本鹌鹑的靶器官是肝脏；亚急性毒性试验的高剂量组鸟的存活率、体重、耗食明显低于对照组，血清胆碱酯酶活性随剂量升高而升高，高剂量组鸟肝细胞脂肪变性；亚慢性毒性试验的各处理组鸟的存活率、体重、耗食与对照相比无明显差异，但血清胆碱酯酶活性显著随剂量升高而升高，中、高剂量组肝细胞不同程度的脂肪变性，氟吗啉高剂量组雌性鹌鹑产蛋数显著小于对照组。在本试验条件下，亚慢性试验最大无作用浓度（NOEC）为250mg/kg饲料浓度。
Avian toxicity is an important aspect of environmental toxicology testing, and has increasingly become a topic of research interest in China. Currently, acute avian toxicity is one of the tests required for pesticide registration in China. Flumorph (SYP-190), a new fluorine-containing fungicide, was developed by the Shenyang Research Institute of Chemical Industry which retains proprietary rights to the compound. Our objective was to establish a set of avian long-term toxicity experiments and to conduct in-depth research on flumorph toxicity. An acute oral LD50 test and a 42-day subacute oral toxicity test were conducted with Japanese quail using direct placement of flumorph into the stomach, to obtain a preliminary assessment of acute and subacute toxicity. Similarly, a 5-day dietary LC50 test and a 90-day subchronic dietary test were conducted with Japanese quail, to determine subacute and subchronic dietary toxicity.The liver was found to be the target organ of flumorph in Japanese quail; in the subacute test, the high rate of flumorph significantly decreased survival, body weight, and food consumption compared to the control group, CHE activity increased as the doses rised,and liver cells showed fatty degeneration; in the subchronic test, flumorph at the high rate did not significantly affect body weight or food consumption, but significantly affect laying rate, and CHE activity significantly increased as the doses rised, and liver cells differently showed fatty degeneration in the middle ,high rate of flumorph , NOEC of flumorph in feed was 250mg/kg.
试验结果表明，氟吗啉作用于日本鹌鹑的靶器官是肝脏；亚急性毒性试验的高剂量组鸟的存活率、体重、耗食明显低于对照组，血清胆碱酯酶活性随剂量升高而升高，高剂量组鸟肝细胞脂肪变性；亚慢性毒性试验的各处理组鸟的存活率、体重、耗食与对照相比无明显差异，但血清胆碱酯酶活性显著随剂量升高而升高，中、高剂量组肝细胞不同程度的脂肪变性，氟吗啉高剂量组雌性鹌鹑产蛋数显著小于对照组。在本试验条件下，亚慢性试验最大无作用浓度（NOEC）为250mg/kg饲料浓度。
Avian toxicity is an important aspect of environmental toxicology testing, and has increasingly become a topic of research interest in China. Currently, acute avian toxicity is one of the tests required for pesticide registration in China. Flumorph (SYP-190), a new fluorine-containing fungicide, was developed by the Shenyang Research Institute of Chemical Industry which retains proprietary rights to the compound. Our objective was to establish a set of avian long-term toxicity experiments and to conduct in-depth research on flumorph toxicity. An acute oral LD50 test and a 42-day subacute oral toxicity test were conducted with Japanese quail using direct placement of flumorph into the stomach, to obtain a preliminary assessment of acute and subacute toxicity. Similarly, a 5-day dietary LC50 test and a 90-day subchronic dietary test were conducted with Japanese quail, to determine subacute and subchronic dietary toxicity.The liver was found to be the target organ of flumorph in Japanese quail; in the subacute test, the high rate of flumorph significantly decreased survival, body weight, and food consumption compared to the control group, CHE activity increased as the doses rised,and liver cells showed fatty degeneration; in the subchronic test, flumorph at the high rate did not significantly affect body weight or food consumption, but significantly affect laying rate, and CHE activity significantly increased as the doses rised, and liver cells differently showed fatty degeneration in the middle ,high rate of flumorph , NOEC of flumorph in feed was 250mg/kg.
引文
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