DEN诱发大鼠肝癌组织中CD117及CD133的表达及意义
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摘要
目的探讨CD117蛋白及CD133蛋白在DEN诱发大鼠肝癌组织中的表达及意义。
方法将20只大鼠随机分为正常组(n=6)、模型组(n=14)。模型组大鼠连续饮用95ug/ml的二乙基亚硝胺(DEN)溶液4周后,改饮用灭菌自来水4周,再继续饮用含95ug/ml的DEN溶液。于造模后14周和18周处死大鼠,取肝组织标本进行病理检查、免疫组化采用SP法,观察CD117及CD133的表达情况。
结果
1.正常组肝细胞排列正常,无变性、坏死。模型组14周3/6只发生肝细胞癌,18周5/6只发生肝细胞癌。
2.正常组在中央静脉、大血管周围及肝实质内可见少量CD117阳性细胞分布,阳性细胞数20±2.4/10~3个:模型组14周癌症及癌症周围组织内阳性细胞数111±16.3/10~3个;18周阳性细胞数94±11.1/10~3个,与正常组比较P＜0.01。
3.正常组在汇管区及中央静脉为中心可见少量CD133阳性细胞,阳性细胞数17±1.4/10~3:模型组14周癌症周围组织内阳性细胞数65±8.0/10~3,18周阳性细胞数88±13.4/10~3,与正常组比较P＜0.01。
4.正常组、模型组肝组织内均可见少量CD117和CD133共同表达的阳性细胞。
结论
1.大鼠肝硬化肝癌组织中存在CD117和CD133表达的卵圆细胞,并与肝硬化及肝癌的发生有关。
2大鼠肝细胞癌发生发展的不同时期存在不同类型的卵圆细胞。
Objective To explore expression and significance.CD117 and CD133 in DEN-induced rat hepatocellular carcinoma.
Methods A total 20 rats were randomly divided into normal group(n=6) and model group(n=14).95ug/ml diethylnitrosamine(DEN) was administrated to the model rats for four weeks,then they were continued to drink tap water that have sterilized for 4 weeks,after the 4 weeks the model groups continue to be consumed with the 95ug/ml Diethylnitrosamine(DEN).The model groups were killed after 14 weeks and 18 weeks,the pathological examination and immunohistochemical SP method were executed in the rats liver of the surgical excision,and the expression CD117 and CD133 Protein was observed.
Results1.Liver cells were normal with no degeneration and necrosis in the normal group of rats.3/6 in the rat model of hepatocellular carcinoma occurred in 14 weeks of the group and 5/6 in the rat model of hepatocellular carcinoma occurred in 18 weeks of the group.
2.A small number of positive cells that was expressed by CD117 could be seen around the central vein,large blood vessels and in the liver parenchyma in the rats liver tissues,the number of positive cells was 20±2.4/10~3.The number of positive cells was 111±16.3/10~3 in 14-week group;and the number of positive cells was 94±11.1/10~3 in 18-week group,there was extremely significant difference between Model group and normal group(P＜0.01).
3.A small number of positive cells that was expressed by CD133 could be seen in the portal area and around the central vein in rat liver,the number of positive cells was 17±1.4/10~3.The number of positive cells was 65±8.0/10~3 in 14-week group;and the number of positive cells was 88±13.4/10~3 in 18-week group,there was extremely significant difference between Model group and normal group(P＜0.01).
4.There were co-expressions of positive cells in a small number of organizations both the normal and model group liver tissue.
Conclusion
1 There are oval cells co-expression of CD117 and CD133 in rats with liver cirrhosis and hepatocellular carcinoma,the oval cells are related with the occurrence of liver cirrhosis and liver cancer.
2 There are different types of oval cells in hepatocellular carcinoma development at different stages in rats.
方法将20只大鼠随机分为正常组(n=6)、模型组(n=14)。模型组大鼠连续饮用95ug/ml的二乙基亚硝胺(DEN)溶液4周后,改饮用灭菌自来水4周,再继续饮用含95ug/ml的DEN溶液。于造模后14周和18周处死大鼠,取肝组织标本进行病理检查、免疫组化采用SP法,观察CD117及CD133的表达情况。
结果
1.正常组肝细胞排列正常,无变性、坏死。模型组14周3/6只发生肝细胞癌,18周5/6只发生肝细胞癌。
2.正常组在中央静脉、大血管周围及肝实质内可见少量CD117阳性细胞分布,阳性细胞数20±2.4/10~3个:模型组14周癌症及癌症周围组织内阳性细胞数111±16.3/10~3个;18周阳性细胞数94±11.1/10~3个,与正常组比较P＜0.01。
3.正常组在汇管区及中央静脉为中心可见少量CD133阳性细胞,阳性细胞数17±1.4/10~3:模型组14周癌症周围组织内阳性细胞数65±8.0/10~3,18周阳性细胞数88±13.4/10~3,与正常组比较P＜0.01。
4.正常组、模型组肝组织内均可见少量CD117和CD133共同表达的阳性细胞。
结论
1.大鼠肝硬化肝癌组织中存在CD117和CD133表达的卵圆细胞,并与肝硬化及肝癌的发生有关。
2大鼠肝细胞癌发生发展的不同时期存在不同类型的卵圆细胞。
Objective To explore expression and significance.CD117 and CD133 in DEN-induced rat hepatocellular carcinoma.
Methods A total 20 rats were randomly divided into normal group(n=6) and model group(n=14).95ug/ml diethylnitrosamine(DEN) was administrated to the model rats for four weeks,then they were continued to drink tap water that have sterilized for 4 weeks,after the 4 weeks the model groups continue to be consumed with the 95ug/ml Diethylnitrosamine(DEN).The model groups were killed after 14 weeks and 18 weeks,the pathological examination and immunohistochemical SP method were executed in the rats liver of the surgical excision,and the expression CD117 and CD133 Protein was observed.
Results1.Liver cells were normal with no degeneration and necrosis in the normal group of rats.3/6 in the rat model of hepatocellular carcinoma occurred in 14 weeks of the group and 5/6 in the rat model of hepatocellular carcinoma occurred in 18 weeks of the group.
2.A small number of positive cells that was expressed by CD117 could be seen around the central vein,large blood vessels and in the liver parenchyma in the rats liver tissues,the number of positive cells was 20±2.4/10~3.The number of positive cells was 111±16.3/10~3 in 14-week group;and the number of positive cells was 94±11.1/10~3 in 18-week group,there was extremely significant difference between Model group and normal group(P＜0.01).
3.A small number of positive cells that was expressed by CD133 could be seen in the portal area and around the central vein in rat liver,the number of positive cells was 17±1.4/10~3.The number of positive cells was 65±8.0/10~3 in 14-week group;and the number of positive cells was 88±13.4/10~3 in 18-week group,there was extremely significant difference between Model group and normal group(P＜0.01).
4.There were co-expressions of positive cells in a small number of organizations both the normal and model group liver tissue.
Conclusion
1 There are oval cells co-expression of CD117 and CD133 in rats with liver cirrhosis and hepatocellular carcinoma,the oval cells are related with the occurrence of liver cirrhosis and liver cancer.
2 There are different types of oval cells in hepatocellular carcinoma development at different stages in rats.
引文
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