几种林木病毒鉴定与林业微生物菌种数据库查询系统构建
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摘要
本实验对几种表现病毒病症状的木本植物样品(包括扶桑、白蜡、丁香、泡桐和枣树)采用生物学、血清学、电镜和分子生物学等技术进行了病毒检测与鉴定。综合各种检测方法得到如下结果：河南五彩扶桑为番茄花叶病毒(ToMV)侵染；北京扶桑、宁夏大叶白蜡、北京大叶白蜡与丁香为烟草花叶病毒(TMV)侵染；河南泡桐为TMV和黄瓜花叶病毒(CMV)复合侵染；枣树花叶病的病原没有得到明确的结论。
ToMV侵染扶桑、TMV侵染扶桑和丁香以及TMV与CMV复合侵染泡桐均为首次报道。
以林业微生物菌种信息为对象，采用计算机网络和数据库技术，构建了基于Web的林业微生物菌种数据库查询系统，主要研究内容包括：确定了架构本系统的IIS5.0+ACCESS+ASP的合理方案。建立了结构适宜的林业微生物菌种数据库和开发系统所需的软硬件结构。设计了高效的菌种信息数据库检索程序，实现了包括菌种菌号、拉丁学名等10个相关信息的模糊查询功能。用Dreamweaver UltraDev 4.0网页开发管理工具设计了系统的网站，并集成了菌种资源查询系统。
基于Web的查询系统能为普通用户提供翔实的菌种信息，并且实现了菌种信息的动态管理，提高了工作效率和工作水平，具有较高的的实用价值。
Some forestial plants infected by viruses, including Hibiscus rosa-sinensis Linn., Fraxinus chinensis, Syringa oblate Lindl., Paulownia and Zizyphus jujube Mill., were detected and identified by bioassay, serology and molecular biological techniques. According to the results of these detection, it revealed that, one Hibiscus rosa-sinensis sample from Henan was infected by ToMV, the other sample from Beijing was infected by TMV, two Fraxinus chinensis samples respectively coming from Ningxia and Beijing were infected by TMV, Syringa oblate Lindl.sample from Beijing was also infected by TMV, Paulownia sample from Henan was infected by CMV and TMV. We are still under the detection and identification about the pathogen from Zizyphus jujube Mill. now.
It were firstly reported about ToMV on Hibiscus rosa-sinensis Linn., TMV on Hibiscus rosa-sinensis Linn, and Syringa oblate Lindl., CMV and TMV on Paulownia.
Taking the forestry microorganisms information as application object, based on computer network and database technology, this dissertation present a research on designing of database index searching for forestry microorganisms. The research consists of such items: The rational scheme of IIS5.0+ ACCESS+ASP was applied to the system. The database of forestry microorganisms was built correctly, and the frameworks of the system's hardware and software were made. The effective retrieve program for the database was designed. A website that integrated with database index searching for forestry microorganisms system was designed, using Dreamweaver UltraDev 4.0, a website development tool.
The system can provide full and accurate forestry microorganism index for the users and provide functions of dynamically managing the database for the administrator.
ToMV侵染扶桑、TMV侵染扶桑和丁香以及TMV与CMV复合侵染泡桐均为首次报道。
以林业微生物菌种信息为对象，采用计算机网络和数据库技术，构建了基于Web的林业微生物菌种数据库查询系统，主要研究内容包括：确定了架构本系统的IIS5.0+ACCESS+ASP的合理方案。建立了结构适宜的林业微生物菌种数据库和开发系统所需的软硬件结构。设计了高效的菌种信息数据库检索程序，实现了包括菌种菌号、拉丁学名等10个相关信息的模糊查询功能。用Dreamweaver UltraDev 4.0网页开发管理工具设计了系统的网站，并集成了菌种资源查询系统。
基于Web的查询系统能为普通用户提供翔实的菌种信息，并且实现了菌种信息的动态管理，提高了工作效率和工作水平，具有较高的的实用价值。
Some forestial plants infected by viruses, including Hibiscus rosa-sinensis Linn., Fraxinus chinensis, Syringa oblate Lindl., Paulownia and Zizyphus jujube Mill., were detected and identified by bioassay, serology and molecular biological techniques. According to the results of these detection, it revealed that, one Hibiscus rosa-sinensis sample from Henan was infected by ToMV, the other sample from Beijing was infected by TMV, two Fraxinus chinensis samples respectively coming from Ningxia and Beijing were infected by TMV, Syringa oblate Lindl.sample from Beijing was also infected by TMV, Paulownia sample from Henan was infected by CMV and TMV. We are still under the detection and identification about the pathogen from Zizyphus jujube Mill. now.
It were firstly reported about ToMV on Hibiscus rosa-sinensis Linn., TMV on Hibiscus rosa-sinensis Linn, and Syringa oblate Lindl., CMV and TMV on Paulownia.
Taking the forestry microorganisms information as application object, based on computer network and database technology, this dissertation present a research on designing of database index searching for forestry microorganisms. The research consists of such items: The rational scheme of IIS5.0+ ACCESS+ASP was applied to the system. The database of forestry microorganisms was built correctly, and the frameworks of the system's hardware and software were made. The effective retrieve program for the database was designed. A website that integrated with database index searching for forestry microorganisms system was designed, using Dreamweaver UltraDev 4.0, a website development tool.
The system can provide full and accurate forestry microorganism index for the users and provide functions of dynamically managing the database for the administrator.
引文
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2. Pringle C R. Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999. Arch. Virol., 1999, 144:2065～2070
3. Mayo M A, Brunt A A. The current state of plant virus taxonomy. Mol. Plant Pathol., 2001, 2:97～100
4. Hamilton R T, Edwardson J R et al. Guidelines for the identification and characterization of plant viruses. Gen. Virol, 1981, 54:223～241
5．胡稳奇．分子生物学技术在植物病毒鉴定与分类中的应用．植物保护，1994，2：31～33
6．候义龙，张开春，胡文玉，等．20世纪果树病毒检测技术的回顾与展望．沈阳农业大学学报，2001，32(2)：144～149
7．洪健，李德葆，周雪平．植物病毒分类图谱(第一版)．北京．科学出版社．2001．12，2
8．徐洁．指示植物鉴定马铃薯病毒技术的研究与应用．中国马铃薯，2002，(2)：73～75
9．刘华．环境条件对马铃薯病毒生物测定结果的影响．山西农业科学，2003，31(2)：72～74
10．陈剑平．胶体金免疫电镜技术检测和鉴定病汁液中不同形态的植物病毒．植物病理学报，1993，23(2)：169～174
11．陈德蕙，张贺秋，王国华．电镜在病毒感染诊断中的应用．电子显微学报，1997，16(5)：549～568
12．彭宏梅，李月秋，张仲凯，等．应用电镜技术研究大理州烟草苗期病毒病的发生情况．云南农业大学学报，2000，15(1)：71～72
13．徐均焕．用A蛋白免疫电镜快速检测几种蔬菜作物的两种杆状病毒．植物病理学报，1996，26(3)：231～236
14．邵碧英．植物病毒分子检测方法概述．植物检疫，2000，(6)：377～379
15. Banttari E E, Goodwin P H. Plant Disease, 1985, 69: 202～205
16．周雪平，濮祖芹．应用斑点法检测植物病毒的研究．病毒学杂志，1990，(3)：317～320
17．陈南海，杨立，裘维藩．一种检测植物病毒的新技术．植物病理学报，1992，22(1)：89～93
18．陈京，宋淑敏，陈燕芳，等．三种植物病毒A蛋白酶联诊断试剂盒的研制简报．植物检疫，1997，11(1)：27
19. Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, 1975, 256: 495～496
20．朱水芳，相宁，张成良，等．PCR和Dig-cDNA探针检测番茄环斑病毒．中国进出境动植检，1995，(4)：29～31
21．李尉民，Roger Hull，张成良，等．南方菜豆花叶病毒(SBMV)两典型株系特异cDNA和RNA探针的制备及应用．植物病理学报，1998，28(3)：243～247
22. Mullis k., et al. Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction. Cold Spring Harbor Symp. Quant.Biol., 1986, 51:263
23．吴乃虎．基因工程原理．第二版，北京：科学出版社，2000
24．梁国栋．最新分子生物学实验技术．北京：科学出版社，2001，129
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