驱杀牛皮蝇蛆最新药剂—敌百虫点涂剂的研究
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摘要
由于牛皮蝇蛆病严重危害养牛业的发展，本实验为了控制和消除牛皮蝇蛆病，根据国内外对透皮吸收理论与透皮吸收促进剂的研究和倍硫磷浇泼剂的成功配方为基础筛选出主药敌百虫、透皮促进剂丙二醇和氮酮，配制出四种不同浓度的驱杀牛皮蝇蛆病新药剂—敌百虫点涂剂(spot on)。在牛体上进行点涂试验后分时段采血，将血样处理后用GC—9A气相色谱仪进行血药浓度测定，与敌百虫注射剂血药浓度测定结果相互比较，最终筛选出7.5％的敌百虫点涂剂做疗效实验。并且与倍硫磷注射剂与倍硫磷浇泼剂的杀虫率比较，结果是7.5％敌百虫点涂剂杀虫率达到100％。杀虫率比倍硫磷注射剂高8.98百分点，比倍硫磷浇泼剂高2.38百分点。
To control bovine hypodermosis which seriously endanger the line of raising cattle Trichlorphon as primary medicine and 1,2-Propylene glycol as well as Azone as enhancer of cutaneous penetration were culled and compounded to 4 density Trichlorphon spot-on according to the theory of percutaneous absorption , the research to enhancer of cutaneous penetration in china and other countries. Trichlorphon spot-on is a new medicine to treat bovine hypodermosis disease. After cattle were daubed with Trichlorphon spot-on , blood samples were collected and handled on different time. By measuring Trichlorphon density of the blood samples with GC-9A gas chromatograph and comparing with the group received an injection of Trichlorphon 7.5% Trichlorphon spot-on was selected as the medicine of treatment test. The clinical test achieved 100% efficacy to kill bovine hypodermosis larvae with 7.5% Trichlorphon spot-on , which exceed 10% than the efficacy of an Fenthion injection and 2% than the of Fenthion pour-on.
To control bovine hypodermosis which seriously endanger the line of raising cattle Trichlorphon as primary medicine and 1,2-Propylene glycol as well as Azone as enhancer of cutaneous penetration were culled and compounded to 4 density Trichlorphon spot-on according to the theory of percutaneous absorption , the research to enhancer of cutaneous penetration in china and other countries. Trichlorphon spot-on is a new medicine to treat bovine hypodermosis disease. After cattle were daubed with Trichlorphon spot-on , blood samples were collected and handled on different time. By measuring Trichlorphon density of the blood samples with GC-9A gas chromatograph and comparing with the group received an injection of Trichlorphon 7.5% Trichlorphon spot-on was selected as the medicine of treatment test. The clinical test achieved 100% efficacy to kill bovine hypodermosis larvae with 7.5% Trichlorphon spot-on , which exceed 10% than the efficacy of an Fenthion injection and 2% than the of Fenthion pour-on.
引文
1 孔繁瑶．家畜寄生虫学(第二版)，北京，中国农业大学出版社，276～277
2 Losson B.,Lonneux J.F.and Liebisch A. CHEMOPRHYLAXIS AND CHEMOTHERAPY OF CATTLE HYPODERMOSIS, agriculture.1998; 62～67
3 斯琴朝日格图等．内蒙古赤峰地区牛皮蝇生活史观察．中国兽医科技，2000；30(1)：26～27
4 王严等．防止北病南移．江西畜牧兽医杂志，1994；1：37～42
5 葡龙等．内蒙古自治区三大外寄生虫病特点．内蒙古兽医，1997；3：40～41
6 莫力根．呼和巴特尔等．牛皮蝇蛆病及其防治，内蒙古人民出版社
7 薛瑞德等．兽医害虫的危害与综合治理．中兽医医药杂志，1994；6：19
8 金春善等．羊感染牛皮蝇蛆病的调查与防治．中国兽医杂志，2000；26(1)：34～35
9 王昆等，纹皮蝇蚴虫侵入人脑内．内蒙古医学院学报，1994；2：13
12 李志文等，纹皮蝇蚴虫致眼损害一例．中华眼科杂志，1995；6：17
13 刚强等，人患皮下蝇蛆病．农垦医学，2000；4：35
12 金兆庆等．阻断牛皮蝇产卵环节防治牛皮蝇蛆病防治研究．中国兽医科技，1995；25(7)：32～33
13 Forsyth B.A,et al,Seasonal variation in anthelmintic reaponce bycattle to dermally applied levamisol,Aust,Vet,J. 1983; 60(5): 141～146
14 Scheuplei R.J.Mechanism of percutaneous absorption,J.Invent.Dermatol, 1967; 48:79～82
15 Duncan C.G.Dynamics and Inipact of tick borne diseases of cattle,Tropical animal health and Production Vol, 1997; 29: NO,4,supp' ement
16 Lonneux J.F.et al. Field effeacy of inectable and pour-on mox-idectin in cattle naturally infested with Psoroptes ovis,Vet.parasitol,1992; 45: 147～152
17 yashi K, Malgoriats S, Howard L,Perctutaneous absorption: a single—layer model. J Pharm Scl, 1993;82(5): 450
18 黄荣等，药物的透皮吸收．阳中医学院学报，2001；2
19 Berry BW. Penetration Enhancer—mode.of action in hu—man skin In Pharmacol. Skin,Karger Baseled. Vol 1,121～137
20 郝轻松等．透皮促进剂对胰岛素离子导入大鼠体内血糖水平的影响．药学学报，1995；30(10)：776
21 Pliquett U,Weaver JC. Teansport of a charged molecule across the human epidermis due to electroporation[J]. J controlled Relase,1996; 38:1
22 Gregor c. Crabieleb,Anderas S et al The skina pathway for syatemic treament with Patches and lipid based agent carries Advanced Drug Delivery. Reviews. 1996; 18: 349
23 丁平田等．透皮吸收药物的载体—传递体．国外医学(合，生，制)，1997；18(1)：48
24 Kiyoshik,J Pharm. Sci, 1993; 82(5): 450
25 李亚芳等．中国药学杂志，1997；32(1)：4
26 陈国神等．国外药学—(合．生．制)分册，1998；9(2)：99
27 宋友华等．中国药学杂志，1991；26(3)：140
28 Pfister WS, et al. Pharm Tech, 1990; 14(9): 132
29 邓树海等．药物动力学与生物药剂学，天津科技翻译出版社，1992；505～507
30 Chier Y.W，透皮释控药剂的进展，国外医学—(合．生．制)分册，1985；6(1)：40～45
31 Bartek M. J. et al. Skin Permebility in vivo: comparison in ret, rab—bit, pig, and man, J。Invest Dermatol, 1972;58:67～70
32 Aunusr BJ. pharm. Res. 1989; 6(3): 224
33 Chulwumerije O, et al. J Invest Derrnatol, 1989; 93(2): 349
34 Schmook FP, et al. Skin pharmacol, 1993: 6(2): 116
35 Hirvonen J, et al. pharm. Res, 1991; 8(7): 993
36 Hrabalek A, et al. pharmazie, 1994; 49(5): 325
37 Wayne TH, et al. Acta. pharm Nord, 1989; (5): 640
38 Loftsson T, et al. Acta pharm Nord, 1995; 84(5): 279
39 Michniak BB, et al. J pharm Sci, 1996; 85(2):150
40 Aoyagi T, et al. Drug Des Discov, 1991; 81(1): 3
41 Sasaki H. et al. J pharmcobiodyn, 1990; 13(3): 200
42 Priborsky J. et al. pharm weekbl Sci, 1998;10950:89
43 KATO A, et al. J pharm phaemacol, 1987; 38:399
44 Bhattachan SN, et al. Int J pharm, 1992; 79: 263
45 Aoyagi T, et al. Drug Des Discov, 1991; 8(1): 47
46 Konda S. et al. J pharmacobiodyn, 1998; 11(2): 88
47 Tsai JC, et al J pharm Sci, 1996; 85(6): 643
48 Patil S, et al. J pharm Sci, 1996; 85(3): 249
49 林宏等．氮酮对14种药物的透皮促进作用．天津药学，1997；9(3)：25～28
50 Michniak B B,player M R, Godwin D A.et al. A study of enhancer structure activity relationships in a series of 2—oxopiperidine—1—acetic acidesters[J]. Research, 1995; 13(9): 268
51 药效资料．月桂氮酮在部分医药中的配方实例
52 Chatterjee D J, Koda R T. Effect of vehicle penetration enhancers on transdermal delivery of methotrexate through hairless mouse skin[J]. pharm Res, 1995; 13(9): 274
53 Michiak. B B. player MR, Godwin DA, et al. skin permeation kinetics of progesterone by binary
mixtures of pdyethylene glycol elhers and isopropyr palmitate[J]. pharm Res, 1995; 13(9): 286
54 张奇等．透皮吸收促进剂对青龙贴中麻黄生物碱透皮吸收的影响[J]中国中药杂志，2000；25(3)：154～156
55 沉奎等．低浓度敌百虫饮水驱虫防治，三蝇防治专题文献资料汇编(二)，1981；46
56 李琳等．透皮促进剂在透皮吸收制剂中的应用，医药导报，2001；6(20)：383
57 呼和巴特尔等．倍硫磷浇泼剂灭杀牛皮蝇蛆的疗效对比试验，驱杀牛皮蝇蛆新药剂—倍硫磷浇泼剂研究，内蒙古农牧学院“倍硫磷浇泼剂课题组”，1996；13～17
58 呼和巴特尔等．倍硫磷浇泼剂防治牛皮蝇蛆病的疗效试验，驱杀牛皮蝇蛆新药剂—倍硫磷浇泼剂研究，内蒙古农牧学院“倍硫磷浇泼剂课题组”，1996；9～12
2 Losson B.,Lonneux J.F.and Liebisch A. CHEMOPRHYLAXIS AND CHEMOTHERAPY OF CATTLE HYPODERMOSIS, agriculture.1998; 62～67
3 斯琴朝日格图等．内蒙古赤峰地区牛皮蝇生活史观察．中国兽医科技，2000；30(1)：26～27
4 王严等．防止北病南移．江西畜牧兽医杂志，1994；1：37～42
5 葡龙等．内蒙古自治区三大外寄生虫病特点．内蒙古兽医，1997；3：40～41
6 莫力根．呼和巴特尔等．牛皮蝇蛆病及其防治，内蒙古人民出版社
7 薛瑞德等．兽医害虫的危害与综合治理．中兽医医药杂志，1994；6：19
8 金春善等．羊感染牛皮蝇蛆病的调查与防治．中国兽医杂志，2000；26(1)：34～35
9 王昆等，纹皮蝇蚴虫侵入人脑内．内蒙古医学院学报，1994；2：13
12 李志文等，纹皮蝇蚴虫致眼损害一例．中华眼科杂志，1995；6：17
13 刚强等，人患皮下蝇蛆病．农垦医学，2000；4：35
12 金兆庆等．阻断牛皮蝇产卵环节防治牛皮蝇蛆病防治研究．中国兽医科技，1995；25(7)：32～33
13 Forsyth B.A,et al,Seasonal variation in anthelmintic reaponce bycattle to dermally applied levamisol,Aust,Vet,J. 1983; 60(5): 141～146
14 Scheuplei R.J.Mechanism of percutaneous absorption,J.Invent.Dermatol, 1967; 48:79～82
15 Duncan C.G.Dynamics and Inipact of tick borne diseases of cattle,Tropical animal health and Production Vol, 1997; 29: NO,4,supp' ement
16 Lonneux J.F.et al. Field effeacy of inectable and pour-on mox-idectin in cattle naturally infested with Psoroptes ovis,Vet.parasitol,1992; 45: 147～152
17 yashi K, Malgoriats S, Howard L,Perctutaneous absorption: a single—layer model. J Pharm Scl, 1993;82(5): 450
18 黄荣等，药物的透皮吸收．阳中医学院学报，2001；2
19 Berry BW. Penetration Enhancer—mode.of action in hu—man skin In Pharmacol. Skin,Karger Baseled. Vol 1,121～137
20 郝轻松等．透皮促进剂对胰岛素离子导入大鼠体内血糖水平的影响．药学学报，1995；30(10)：776
21 Pliquett U,Weaver JC. Teansport of a charged molecule across the human epidermis due to electroporation[J]. J controlled Relase,1996; 38:1
22 Gregor c. Crabieleb,Anderas S et al The skina pathway for syatemic treament with Patches and lipid based agent carries Advanced Drug Delivery. Reviews. 1996; 18: 349
23 丁平田等．透皮吸收药物的载体—传递体．国外医学(合，生，制)，1997；18(1)：48
24 Kiyoshik,J Pharm. Sci, 1993; 82(5): 450
25 李亚芳等．中国药学杂志，1997；32(1)：4
26 陈国神等．国外药学—(合．生．制)分册，1998；9(2)：99
27 宋友华等．中国药学杂志，1991；26(3)：140
28 Pfister WS, et al. Pharm Tech, 1990; 14(9): 132
29 邓树海等．药物动力学与生物药剂学，天津科技翻译出版社，1992；505～507
30 Chier Y.W，透皮释控药剂的进展，国外医学—(合．生．制)分册，1985；6(1)：40～45
31 Bartek M. J. et al. Skin Permebility in vivo: comparison in ret, rab—bit, pig, and man, J。Invest Dermatol, 1972;58:67～70
32 Aunusr BJ. pharm. Res. 1989; 6(3): 224
33 Chulwumerije O, et al. J Invest Derrnatol, 1989; 93(2): 349
34 Schmook FP, et al. Skin pharmacol, 1993: 6(2): 116
35 Hirvonen J, et al. pharm. Res, 1991; 8(7): 993
36 Hrabalek A, et al. pharmazie, 1994; 49(5): 325
37 Wayne TH, et al. Acta. pharm Nord, 1989; (5): 640
38 Loftsson T, et al. Acta pharm Nord, 1995; 84(5): 279
39 Michniak BB, et al. J pharm Sci, 1996; 85(2):150
40 Aoyagi T, et al. Drug Des Discov, 1991; 81(1): 3
41 Sasaki H. et al. J pharmcobiodyn, 1990; 13(3): 200
42 Priborsky J. et al. pharm weekbl Sci, 1998;10950:89
43 KATO A, et al. J pharm phaemacol, 1987; 38:399
44 Bhattachan SN, et al. Int J pharm, 1992; 79: 263
45 Aoyagi T, et al. Drug Des Discov, 1991; 8(1): 47
46 Konda S. et al. J pharmacobiodyn, 1998; 11(2): 88
47 Tsai JC, et al J pharm Sci, 1996; 85(6): 643
48 Patil S, et al. J pharm Sci, 1996; 85(3): 249
49 林宏等．氮酮对14种药物的透皮促进作用．天津药学，1997；9(3)：25～28
50 Michniak B B,player M R, Godwin D A.et al. A study of enhancer structure activity relationships in a series of 2—oxopiperidine—1—acetic acidesters[J]. Research, 1995; 13(9): 268
51 药效资料．月桂氮酮在部分医药中的配方实例
52 Chatterjee D J, Koda R T. Effect of vehicle penetration enhancers on transdermal delivery of methotrexate through hairless mouse skin[J]. pharm Res, 1995; 13(9): 274
53 Michiak. B B. player MR, Godwin DA, et al. skin permeation kinetics of progesterone by binary
mixtures of pdyethylene glycol elhers and isopropyr palmitate[J]. pharm Res, 1995; 13(9): 286
54 张奇等．透皮吸收促进剂对青龙贴中麻黄生物碱透皮吸收的影响[J]中国中药杂志，2000；25(3)：154～156
55 沉奎等．低浓度敌百虫饮水驱虫防治，三蝇防治专题文献资料汇编(二)，1981；46
56 李琳等．透皮促进剂在透皮吸收制剂中的应用，医药导报，2001；6(20)：383
57 呼和巴特尔等．倍硫磷浇泼剂灭杀牛皮蝇蛆的疗效对比试验，驱杀牛皮蝇蛆新药剂—倍硫磷浇泼剂研究，内蒙古农牧学院“倍硫磷浇泼剂课题组”，1996；13～17
58 呼和巴特尔等．倍硫磷浇泼剂防治牛皮蝇蛆病的疗效试验，驱杀牛皮蝇蛆新药剂—倍硫磷浇泼剂研究，内蒙古农牧学院“倍硫磷浇泼剂课题组”，1996；9～12