头针治疗失眠的临床及神经生化机制研究
摘要
目的：
观察头针治疗失眠的临床疗效。同时,通过动物实验,探讨应用头针对腹腔注射对氯苯丙氨酸(PCPA)所致失眠大鼠睡眠的影响,及对大鼠脑组织内免疫因子IL-1β、1L-6.TNF-a浓度的影响。
方法：
本课题研究分为临床研究及动物实验研究两个方面：
(1)临床研究
将符合纳入标准的60例失眠患者按照随机数字法分组、对照、单盲的原则分为两组,其中头针组30例,西药组30例。
具体治疗方法如下：
头针组取穴：主穴：百会、四神聪、神庭、印堂。配穴：阴虚火旺取双侧复溜、内关、三阴交；心脾两虚加双侧心俞、脾俞、膈俞；心胆气虚加双侧心俞、胆俞、膈俞；肝郁化火加双侧太冲、风池、复溜；痰热内扰加双侧丰隆、曲池、内庭；胃腑不和加双侧内关、足三里、公孙。留针30min。每日1次,星期天休息,1周为1个疗程,治疗2个疗程。
西药组：舒乐安定片(艾司唑仑片),湖北制药有限公司生产,国药准字H42021522,购自广州中医药大学第一附属医院门诊药房。每晚睡前30min由患者自行口服,每次lmg,依患者实际情况,可酌加为2mg／次。两周为一个疗程,共2个疗程。
经过2周治疗后,评定两组患者临床症状、中医证候学症状、睡眠效率、匹兹堡睡眠质量指数等方面的变化。
(2)动物实验
采用腹腔注射PCPA混悬液法致大鼠失眠模型,造模成功后,将符合实验要求的SD大鼠40只,按随机数字表随机分为4组,即空白对照组(10只)、模型组(10只)、头针组(10只)、西药组(10只)。具体实验操作方案进行如下：
A.空白对照组：一般环境下饲养大鼠。不造模,不做任何治疗。
B.模型组：采用PCPA灌注法造模成功。于每天上午,取大鼠俯卧位,固定四肢于普通鼠笼的网状笼盖上15min。不做任何治疗。每日1次,共6天。
C.西药组：大鼠10只,于实验第1天造模,造模方法同模型组,实验第3天开始安定注射液腹腔注射治疗。安定(艾司唑仑)片,湖北制药有限公司生产,国药准字H42021522,购自广州中医药大学第一附属医院门诊药房。按成人催眠剂量lmg/天,用人鼠给药剂量体表面积折算公式计算出大鼠给药剂量为0.92mg/kg。每日上午9：00-9：30给大鼠灌胃。每日1次,连续治疗6天。
D.头针组：取穴：百会(顶骨正中,向后斜刺5mm)、印堂(两眼瞳孔连线中点上1.5cm)。留针15min。每日1次,共6天。
在造模后至针刺治疗期间,分别对各组大鼠进行行为学评估,包括大鼠睡眠持续时间、攻击行为、兴奋程度、白天活动度、精神亢奋或倦怠、安静程度、易惊醒度、饮食及二便量。
在造模第8天,将大鼠脱颈椎处死,取出脑组织,干净,研磨,离心,沉淀,提取上清液,采用酶联免疫法检测针刺治疗后大鼠脑组织内细胞因子IL-1β、1L-6、 TNF-a含量。
结果：
(1)失眠临床症状各项观察指标的评分
两组治疗后与治疗前失眠临床症状观察指标评分经秩和检验,除总分项P值大于0.05,余项P值均小于0.05,差异具有统计学意义。两组治疗后失眠临床症状观察指标评分经秩和检验,入睡时间、持续时间、夜醒次数、做梦情况项P值均大于0.05,差异无统计学意义,提示头针治疗与西药治疗在改善以上失眠临床症状方面疗效相当,而醒后状态、睡眠评价、总分项P值均小于0.01,差异具有显著统计学意义,提示头针治疗在改善醒后状态方面,疗效优于西药治疗。
(2)失眠中医证候学各项指标的评分
两组治疗后与治疗前失眠中医证候学指标评分经秩和检验,除总分项P值大于0.05,余项P值均小于0.05,差异具有统计学意义。两组治疗后失眠中医证候学指标评分经秩和检验,入睡困难、多梦易醒、心悸易惊、汗出、注意力不集中、咽干项P值均大于0.05,差异无统计学意义,提示头针治疗与西药治疗在改善以上失眠临床中医证候方面疗效相当,而晨起困倦、四肢倦怠、健忘、烦躁、总分项P值均小于0.01,差异具有显著统计学意义,提示头针治疗在改善晨起困倦、四肢倦怠、健忘、烦躁方面,疗效优于西药治疗。
(3)匹兹堡睡眠质量指数的评分
两组治疗后与治疗前匹兹堡睡眠质量指数评分经秩和检验,除总分项P值大于0.05,余项P值均小于0.05,差异具有统计学意义。两组治疗后失眠匹兹堡睡眠质量指数评分经秩和检验,睡眠时间、睡眠效率项P值均大于0.05,差异无统计学意义,提示头针治疗与西药治疗在改善以上失眠PSQI量表指征方面疗效相当,而睡眠质量、入睡时间、睡眠障碍、催眠药物、日间功能障碍、总分项P值均小于0.01,差异具有显著统计学意义,提示头针治疗在改善睡眠质量、入睡时间、睡眠障碍、催眠药物、日间功能障碍方面,疗效优于西药治疗。
(4)头针治疗失眠的临床疗效比较
将两组的临床疗效进行对比观察及评价。结果：头针组30例失眠患者中痊愈18例(60.00%),显效6例(20.00%),有效4例(13.33%),无效2例(6.67%),总有效率为93.33%。西药组30例失眠患者中痊愈(26.67%)，显效9例(30.00%),有效7例(23.33%),无效6例(20.00%),总有效率为80%。经统计学分析,差异无统计学意义(P<0.05),结论：头针治疗失眠临床总有效率高于西药治疗失眠,但无统计学意义。
(5)脑组织内细胞因子IL-1β浓度
头针组与空白对照组、模型组、西药组失眠大鼠治疗后脑组织IL-1β浓度经One-Way ANOVA分析,P<0.01,差异具有显著统计学意义,提示头针治疗后失眠大鼠脑组织工L-1β显著升高。
(6)脑组织内细胞因子IL-6浓度
头针组与空白对照组、模型组、西药组失眠大鼠治疗后脑组织IL-6浓度经One-Way ANOVA分析,P<0.01,差异具有显著统计学意义,提示头针治疗后失眠大鼠脑组织工L-6显著升高。
(7)脑组织内细胞因子TNF-α浓度
头针组与空白对照组、模型组、西药组失眠大鼠治疗后脑组织TNF-α浓度经One-Way ANOVA分析,P<0.01,差异具有显著统计学意义,提示头针治疗后失眠大鼠脑组织TNF-α显著升高。
结论：
(1)头针治疗失眠及西药治疗失眠均能改善失眠患者的睡眠,但头针治疗失眠在改善醒后状态、晨起困倦、四肢倦怠、健忘、烦躁、睡眠质量、入睡时间、睡眠障碍、催眠药物、日间功能障碍等方面,较西药治疗失眠疗效更显著。同时,具有睡眠质量评价高、临床痊愈率高、临床总有效率高的优势。
(2)头针治疗、西药治疗均能在一定程度上增加失眠大鼠脑组织内细胞因子IL-1β、TNF-α、IL-6的浓度,但是头针治疗大鼠失眠增加大鼠脑组织内细胞因子浓度较西药治疗更为显著。模型组大鼠在造模后存在一定程度上的自然恢复。
Object ive
To observe the effects of scalp acupuncture acting on patients with insomnia. At the same time, by animal experiment study, To explore the effects of Scalp acupuncture acting on insomnia rats after Intraperitoneal injection of para-chlorophenylalanine (PCPA), and the effects of the content of the Cytokine IL-1β、1L-6、TNF-α in the brains of rats after Acupuncture treatment。
Methods
This research is divided into two parts:clinical study and animal experimental study:
(1) Clinical study
60Patients with insomnia were assigned to the treatment group (Scalp acupuncture group) and control group (Drug group) according to the principle of simple random, control and blindness. There were30patients in the treatment group (Scalp acupuncture group) and30patients in the control group (Drug group)
The specific treatment methods are as follows:
Scalp acupuncture group:prescription:the main points:Baihui Sishencong,Shenting,Yintang.The supplementary points:Hyperactivity of fire due to yin deficiency:Fuliu, Neiguan, Sanyinjiao; Deficiency of the heart and spleen:Xinshu, Pishu, Geshu; Deficiency of the heart-qi and gallbladder-qi:Xinshu, Danshu, Geshu; Hyperactivity of fire due to liver-qi depression:Taichong, Fengchi, Fuliu; Phlegm-heat accumulation: Fenglong, Quchi, Neiting; Imblance of the stomach:Neiguan, Zusanli, Gongsun,, The supplementary points are on both sides. Retaining needle time:30min Once a day, rest on Sunday.
Drug group:estazolam tablets, Made in Hubei province (H42021522), Purchased from Guangzhou University of Traditional Chinese Medicine, first affiliated Hospital outpatient service pharmacy, lmg a day, take it before sleep, or2mg a day when needed. One week is a course of treatment, A total of2weeks.
After2weeks of treatment, all of the patients were evaluated through clinical symptoms, syndrome of TCM, sleep efficiency, PSQI.
(2) Animal experimental study
Establishing the animal model of insomnia by injecting para-chlorophenylalanine (PCPA) into the rats abdominal cavity. After rat model established, they were randomly divided into4groups:Blank control group (n=10), Model group(n=10), Scalp acupuncture group(n=10), Drug group(n=10).
The specific treatment methods are as follows:
A. Blank control group:Feeding rats in General environment. NO modeling, NO treating.
B. Model group:Making rat model succefully. Fixed in the rat in homemade cage for15min every morning. NO treating. Once a day, total to6days.
C. Drug group:Making rat model succefully. Estazolam tablets, Made in Hubei province (H42021522), Purchased from Guangzhou University of Traditional Chinese Medicine, first affiliated Hospital outpatient service pharmacy, lmg a day, Employing mouse dose of body surface area formula to calculate the dose in rats0.92mg/kg, Gavage it to rats during. Once a day, total to6days.
D. Scalp acupuncture group:Making rat model succefully. Fixed in the rat homemade cage for15min every morning. Acupuncture prescription:Baihui, Yintang. Once a day, total to6days.
During the experiment, the sleep behavioral of rat of every group were evaluated,the terms such as:Sleep duration, aggressive behaviour, Degree of excitement, Degree of movement during the activity, High spirits or burnout, Degree of quiet, Easy to wake up or not, Diet, Amount of business.
The animals were decapitated8th day after made model, removing the brain tissue.cleaning, grinding, centrifugation, Extracting supernatant fluid, and ELISA method was used to detect the content of the Cytokine IL-1β、1L-6、TNF-α in the brains of rats after Acupuncture treatment。
Results
(1) The scores of clinical symptoms assessment
After the treatment,the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as sleep latency, duration, number of night waking, dreaming are of no significant difference (P>0.05), while the value of P of the items such as the state after wake up, sleep evaluation, total scores are of significant difference (P<0.01), it show us thatrscalp acupuncture therapy is superior to drug therapy in improving symptoms.
(2) The scores of syndrome of TCM assessment
After the treatment, the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as difficulty in falling asleep, more than a dream, Palpitations easy to panic, sweating, difficulty in concentrating, dry throat are of no significant difference (P>0.05), while the value of P of the items such as Early morning sleepiness, fatigue limbs, forgetfulness, irritability total scores are of significant difference (P<0.01), it show us that:scalp acupuncture therapy is superior to drug therapy in improving symptoms.
(3) The scores of PSQI assessment
After the treatment, the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as sleep time, sleep efficiency are of no significant difference (P>0.05), while the value of P of the items such as sleep quality, time of falling asleep, sleep disorders, hypnotic drugs, daytime dysfunction, total scores are of significant difference (P<0.01), it show us that:scalp acupuncture therapy is superior to drug therapy in improving symptoms.
(4) Clinical effective Comparison
Comparing two groups of clinical curative effect. Result:in Scalp acupuncture group,18cases(60.00%) were healed,6cases (20.00%) were significant effective,4cases(13.33%)were effective,2cases (6.67%) were invalid. The effective rate is93.33%. In body acupuncture group,8cases(26.67%) were healed,9cases (30.00%) were significant effective,7cases(23.33%)were effective,6cases (20.00%) were invalid. The effective rate is80%. By statistical analysis, the difference petween two groups is of statistical significance (P<0.05). Conclusion:Scalp acupuncture is superior to treat patients with insomnia than drug therapy.(5) The content of the Cytokine IL-1β in the brains of rats
Compared with the blank group, model group, drug group through One-Way ANOVA, the content of the cytokine IL-1β in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of IL-1β.
(6) The content of the Cytokine TNF-α in the brains of rats
Compared with the blank group, model group, drug group through One-Way ANOVA, the content of the cytokine TNF-α in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of TNF-α.
(7) The content of the Cytokine1L-6in the brains of rats
Compared with the blank group, model group, drug group through One-Way ANOVA,the content of the cytokine1L-6in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of1L-6.
Conclusion
(1) The two therapy both can improve the insomnia, however, The efficacy of scalp acupuncture is more significant than the efficacy of body acupuncture in improving the state after wake up, sleep evaluation, Early morning sleepiness, fatigue limbs, forgetfulness, irritability, sleep quality, time of falling asleep, sleep disorders, hypnotic drugs, daytime dysfunction, total scores.
(2)The two acupuncture therapies can promote the content of the cytokine IL-1β、1L-6、TNF-α in the brains of rats. But the efficacy of scalp acupuncture is more significant than body acupuncture. And the model group recovered automatic in some extent.
观察头针治疗失眠的临床疗效。同时,通过动物实验,探讨应用头针对腹腔注射对氯苯丙氨酸(PCPA)所致失眠大鼠睡眠的影响,及对大鼠脑组织内免疫因子IL-1β、1L-6.TNF-a浓度的影响。
方法：
本课题研究分为临床研究及动物实验研究两个方面：
(1)临床研究
将符合纳入标准的60例失眠患者按照随机数字法分组、对照、单盲的原则分为两组,其中头针组30例,西药组30例。
具体治疗方法如下：
头针组取穴：主穴：百会、四神聪、神庭、印堂。配穴：阴虚火旺取双侧复溜、内关、三阴交；心脾两虚加双侧心俞、脾俞、膈俞；心胆气虚加双侧心俞、胆俞、膈俞；肝郁化火加双侧太冲、风池、复溜；痰热内扰加双侧丰隆、曲池、内庭；胃腑不和加双侧内关、足三里、公孙。留针30min。每日1次,星期天休息,1周为1个疗程,治疗2个疗程。
西药组：舒乐安定片(艾司唑仑片),湖北制药有限公司生产,国药准字H42021522,购自广州中医药大学第一附属医院门诊药房。每晚睡前30min由患者自行口服,每次lmg,依患者实际情况,可酌加为2mg／次。两周为一个疗程,共2个疗程。
经过2周治疗后,评定两组患者临床症状、中医证候学症状、睡眠效率、匹兹堡睡眠质量指数等方面的变化。
(2)动物实验
采用腹腔注射PCPA混悬液法致大鼠失眠模型,造模成功后,将符合实验要求的SD大鼠40只,按随机数字表随机分为4组,即空白对照组(10只)、模型组(10只)、头针组(10只)、西药组(10只)。具体实验操作方案进行如下：
A.空白对照组：一般环境下饲养大鼠。不造模,不做任何治疗。
B.模型组：采用PCPA灌注法造模成功。于每天上午,取大鼠俯卧位,固定四肢于普通鼠笼的网状笼盖上15min。不做任何治疗。每日1次,共6天。
C.西药组：大鼠10只,于实验第1天造模,造模方法同模型组,实验第3天开始安定注射液腹腔注射治疗。安定(艾司唑仑)片,湖北制药有限公司生产,国药准字H42021522,购自广州中医药大学第一附属医院门诊药房。按成人催眠剂量lmg/天,用人鼠给药剂量体表面积折算公式计算出大鼠给药剂量为0.92mg/kg。每日上午9：00-9：30给大鼠灌胃。每日1次,连续治疗6天。
D.头针组：取穴：百会(顶骨正中,向后斜刺5mm)、印堂(两眼瞳孔连线中点上1.5cm)。留针15min。每日1次,共6天。
在造模后至针刺治疗期间,分别对各组大鼠进行行为学评估,包括大鼠睡眠持续时间、攻击行为、兴奋程度、白天活动度、精神亢奋或倦怠、安静程度、易惊醒度、饮食及二便量。
在造模第8天,将大鼠脱颈椎处死,取出脑组织,干净,研磨,离心,沉淀,提取上清液,采用酶联免疫法检测针刺治疗后大鼠脑组织内细胞因子IL-1β、1L-6、 TNF-a含量。
结果：
(1)失眠临床症状各项观察指标的评分
两组治疗后与治疗前失眠临床症状观察指标评分经秩和检验,除总分项P值大于0.05,余项P值均小于0.05,差异具有统计学意义。两组治疗后失眠临床症状观察指标评分经秩和检验,入睡时间、持续时间、夜醒次数、做梦情况项P值均大于0.05,差异无统计学意义,提示头针治疗与西药治疗在改善以上失眠临床症状方面疗效相当,而醒后状态、睡眠评价、总分项P值均小于0.01,差异具有显著统计学意义,提示头针治疗在改善醒后状态方面,疗效优于西药治疗。
(2)失眠中医证候学各项指标的评分
两组治疗后与治疗前失眠中医证候学指标评分经秩和检验,除总分项P值大于0.05,余项P值均小于0.05,差异具有统计学意义。两组治疗后失眠中医证候学指标评分经秩和检验,入睡困难、多梦易醒、心悸易惊、汗出、注意力不集中、咽干项P值均大于0.05,差异无统计学意义,提示头针治疗与西药治疗在改善以上失眠临床中医证候方面疗效相当,而晨起困倦、四肢倦怠、健忘、烦躁、总分项P值均小于0.01,差异具有显著统计学意义,提示头针治疗在改善晨起困倦、四肢倦怠、健忘、烦躁方面,疗效优于西药治疗。
(3)匹兹堡睡眠质量指数的评分
两组治疗后与治疗前匹兹堡睡眠质量指数评分经秩和检验,除总分项P值大于0.05,余项P值均小于0.05,差异具有统计学意义。两组治疗后失眠匹兹堡睡眠质量指数评分经秩和检验,睡眠时间、睡眠效率项P值均大于0.05,差异无统计学意义,提示头针治疗与西药治疗在改善以上失眠PSQI量表指征方面疗效相当,而睡眠质量、入睡时间、睡眠障碍、催眠药物、日间功能障碍、总分项P值均小于0.01,差异具有显著统计学意义,提示头针治疗在改善睡眠质量、入睡时间、睡眠障碍、催眠药物、日间功能障碍方面,疗效优于西药治疗。
(4)头针治疗失眠的临床疗效比较
将两组的临床疗效进行对比观察及评价。结果：头针组30例失眠患者中痊愈18例(60.00%),显效6例(20.00%),有效4例(13.33%),无效2例(6.67%),总有效率为93.33%。西药组30例失眠患者中痊愈(26.67%)，显效9例(30.00%),有效7例(23.33%),无效6例(20.00%),总有效率为80%。经统计学分析,差异无统计学意义(P<0.05),结论：头针治疗失眠临床总有效率高于西药治疗失眠,但无统计学意义。
(5)脑组织内细胞因子IL-1β浓度
头针组与空白对照组、模型组、西药组失眠大鼠治疗后脑组织IL-1β浓度经One-Way ANOVA分析,P<0.01,差异具有显著统计学意义,提示头针治疗后失眠大鼠脑组织工L-1β显著升高。
(6)脑组织内细胞因子IL-6浓度
头针组与空白对照组、模型组、西药组失眠大鼠治疗后脑组织IL-6浓度经One-Way ANOVA分析,P<0.01,差异具有显著统计学意义,提示头针治疗后失眠大鼠脑组织工L-6显著升高。
(7)脑组织内细胞因子TNF-α浓度
头针组与空白对照组、模型组、西药组失眠大鼠治疗后脑组织TNF-α浓度经One-Way ANOVA分析,P<0.01,差异具有显著统计学意义,提示头针治疗后失眠大鼠脑组织TNF-α显著升高。
结论：
(1)头针治疗失眠及西药治疗失眠均能改善失眠患者的睡眠,但头针治疗失眠在改善醒后状态、晨起困倦、四肢倦怠、健忘、烦躁、睡眠质量、入睡时间、睡眠障碍、催眠药物、日间功能障碍等方面,较西药治疗失眠疗效更显著。同时,具有睡眠质量评价高、临床痊愈率高、临床总有效率高的优势。
(2)头针治疗、西药治疗均能在一定程度上增加失眠大鼠脑组织内细胞因子IL-1β、TNF-α、IL-6的浓度,但是头针治疗大鼠失眠增加大鼠脑组织内细胞因子浓度较西药治疗更为显著。模型组大鼠在造模后存在一定程度上的自然恢复。
Object ive
To observe the effects of scalp acupuncture acting on patients with insomnia. At the same time, by animal experiment study, To explore the effects of Scalp acupuncture acting on insomnia rats after Intraperitoneal injection of para-chlorophenylalanine (PCPA), and the effects of the content of the Cytokine IL-1β、1L-6、TNF-α in the brains of rats after Acupuncture treatment。
Methods
This research is divided into two parts:clinical study and animal experimental study:
(1) Clinical study
60Patients with insomnia were assigned to the treatment group (Scalp acupuncture group) and control group (Drug group) according to the principle of simple random, control and blindness. There were30patients in the treatment group (Scalp acupuncture group) and30patients in the control group (Drug group)
The specific treatment methods are as follows:
Scalp acupuncture group:prescription:the main points:Baihui Sishencong,Shenting,Yintang.The supplementary points:Hyperactivity of fire due to yin deficiency:Fuliu, Neiguan, Sanyinjiao; Deficiency of the heart and spleen:Xinshu, Pishu, Geshu; Deficiency of the heart-qi and gallbladder-qi:Xinshu, Danshu, Geshu; Hyperactivity of fire due to liver-qi depression:Taichong, Fengchi, Fuliu; Phlegm-heat accumulation: Fenglong, Quchi, Neiting; Imblance of the stomach:Neiguan, Zusanli, Gongsun,, The supplementary points are on both sides. Retaining needle time:30min Once a day, rest on Sunday.
Drug group:estazolam tablets, Made in Hubei province (H42021522), Purchased from Guangzhou University of Traditional Chinese Medicine, first affiliated Hospital outpatient service pharmacy, lmg a day, take it before sleep, or2mg a day when needed. One week is a course of treatment, A total of2weeks.
After2weeks of treatment, all of the patients were evaluated through clinical symptoms, syndrome of TCM, sleep efficiency, PSQI.
(2) Animal experimental study
Establishing the animal model of insomnia by injecting para-chlorophenylalanine (PCPA) into the rats abdominal cavity. After rat model established, they were randomly divided into4groups:Blank control group (n=10), Model group(n=10), Scalp acupuncture group(n=10), Drug group(n=10).
The specific treatment methods are as follows:
A. Blank control group:Feeding rats in General environment. NO modeling, NO treating.
B. Model group:Making rat model succefully. Fixed in the rat in homemade cage for15min every morning. NO treating. Once a day, total to6days.
C. Drug group:Making rat model succefully. Estazolam tablets, Made in Hubei province (H42021522), Purchased from Guangzhou University of Traditional Chinese Medicine, first affiliated Hospital outpatient service pharmacy, lmg a day, Employing mouse dose of body surface area formula to calculate the dose in rats0.92mg/kg, Gavage it to rats during. Once a day, total to6days.
D. Scalp acupuncture group:Making rat model succefully. Fixed in the rat homemade cage for15min every morning. Acupuncture prescription:Baihui, Yintang. Once a day, total to6days.
During the experiment, the sleep behavioral of rat of every group were evaluated,the terms such as:Sleep duration, aggressive behaviour, Degree of excitement, Degree of movement during the activity, High spirits or burnout, Degree of quiet, Easy to wake up or not, Diet, Amount of business.
The animals were decapitated8th day after made model, removing the brain tissue.cleaning, grinding, centrifugation, Extracting supernatant fluid, and ELISA method was used to detect the content of the Cytokine IL-1β、1L-6、TNF-α in the brains of rats after Acupuncture treatment。
Results
(1) The scores of clinical symptoms assessment
After the treatment,the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as sleep latency, duration, number of night waking, dreaming are of no significant difference (P>0.05), while the value of P of the items such as the state after wake up, sleep evaluation, total scores are of significant difference (P<0.01), it show us thatrscalp acupuncture therapy is superior to drug therapy in improving symptoms.
(2) The scores of syndrome of TCM assessment
After the treatment, the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as difficulty in falling asleep, more than a dream, Palpitations easy to panic, sweating, difficulty in concentrating, dry throat are of no significant difference (P>0.05), while the value of P of the items such as Early morning sleepiness, fatigue limbs, forgetfulness, irritability total scores are of significant difference (P<0.01), it show us that:scalp acupuncture therapy is superior to drug therapy in improving symptoms.
(3) The scores of PSQI assessment
After the treatment, the scores of the two group was tested by Rank sum test, all the value of are lower than that before the treatment, except the total scores. Compared between the two group, the value of P of the items such as sleep time, sleep efficiency are of no significant difference (P>0.05), while the value of P of the items such as sleep quality, time of falling asleep, sleep disorders, hypnotic drugs, daytime dysfunction, total scores are of significant difference (P<0.01), it show us that:scalp acupuncture therapy is superior to drug therapy in improving symptoms.
(4) Clinical effective Comparison
Comparing two groups of clinical curative effect. Result:in Scalp acupuncture group,18cases(60.00%) were healed,6cases (20.00%) were significant effective,4cases(13.33%)were effective,2cases (6.67%) were invalid. The effective rate is93.33%. In body acupuncture group,8cases(26.67%) were healed,9cases (30.00%) were significant effective,7cases(23.33%)were effective,6cases (20.00%) were invalid. The effective rate is80%. By statistical analysis, the difference petween two groups is of statistical significance (P<0.05). Conclusion:Scalp acupuncture is superior to treat patients with insomnia than drug therapy.(5) The content of the Cytokine IL-1β in the brains of rats
Compared with the blank group, model group, drug group through One-Way ANOVA, the content of the cytokine IL-1β in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of IL-1β.
(6) The content of the Cytokine TNF-α in the brains of rats
Compared with the blank group, model group, drug group through One-Way ANOVA, the content of the cytokine TNF-α in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of TNF-α.
(7) The content of the Cytokine1L-6in the brains of rats
Compared with the blank group, model group, drug group through One-Way ANOVA,the content of the cytokine1L-6in the brains of rats was obviously increased, the difference is of statistical significance (P<0.01), it show us that scalp therapy can increase the content of1L-6.
Conclusion
(1) The two therapy both can improve the insomnia, however, The efficacy of scalp acupuncture is more significant than the efficacy of body acupuncture in improving the state after wake up, sleep evaluation, Early morning sleepiness, fatigue limbs, forgetfulness, irritability, sleep quality, time of falling asleep, sleep disorders, hypnotic drugs, daytime dysfunction, total scores.
(2)The two acupuncture therapies can promote the content of the cytokine IL-1β、1L-6、TNF-α in the brains of rats. But the efficacy of scalp acupuncture is more significant than body acupuncture. And the model group recovered automatic in some extent.
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