白念珠菌静脉感染对脾虚小鼠致病性的研究
摘要
目的
研究白念珠菌对脾虚状态下小鼠的致病性，分析脾虚小鼠对白念珠菌免疫力的变化情况及其发病机制，探讨不同免疫状态与白念珠菌感染的关系，为临床防治白念珠菌感染提供实验依据。
方法
采用番泻叶泻下法制备脾虚小鼠模型。经尾静脉接种白念珠菌，观察脾虚小鼠和正常小鼠的平均生存天数。用血清溶血素法测定小鼠体液免疫功能，用碳粒廓清法测定小鼠单核吞噬细胞功能，并测定小鼠肾脏内白念珠菌活菌数，最后比较脾虚小鼠和正常小鼠上述指标的差异。
结果
与正常小鼠相比，脾虚小鼠在感染白念珠菌后生存天数明显缩短，肾脏内白念珠菌活菌菌数明显增多；脾虚小鼠体内抗体生成减少，单核吞噬细胞吞噬功能下降；白念珠菌能够抑制正常小鼠的免疫功能，表现为体内抗体生成减少，单核吞噬细胞吞噬功能下降，使小鼠抵抗力降低。
结论
脾虚小鼠对白念珠菌易感性增加，免疫力减弱，这与脾虚小鼠体内抗体生成减少，单核吞噬细胞吞噬功能下降有关。
Objective
In order to observe the response of spleen deficiency mice to intravenous infection of C.albicans.
Methods
The mice were fed with senna leaf decoction for seven days until the mice appeared some symptoms, such as diarrhea, bad appetite, emaciation and inactivity. Then the mice were injected C.albicans intravenously. The survival days of mice were observed for three weeks and the amount of live C.albicans in kidneys was calculated. The carbon particle clearance test and serum concentration of hemolysin were determined.
Results
The results suggest that C.albicans can suppress the immune function of the normal mice significantly. The phagocytosis of macrophages and the production of antibodies fell obviously. The survival days of senna-treated mice were sharply shorted after the infection of C.albicans and the amount of C.albicans in kidneys was increased. In contrast, the survival days of the normal mice were not different after the infection of C.albicans.
Conclusion
It means that C.albicans is lethal to spleen deficiency mice.
研究白念珠菌对脾虚状态下小鼠的致病性，分析脾虚小鼠对白念珠菌免疫力的变化情况及其发病机制，探讨不同免疫状态与白念珠菌感染的关系，为临床防治白念珠菌感染提供实验依据。
方法
采用番泻叶泻下法制备脾虚小鼠模型。经尾静脉接种白念珠菌，观察脾虚小鼠和正常小鼠的平均生存天数。用血清溶血素法测定小鼠体液免疫功能，用碳粒廓清法测定小鼠单核吞噬细胞功能，并测定小鼠肾脏内白念珠菌活菌数，最后比较脾虚小鼠和正常小鼠上述指标的差异。
结果
与正常小鼠相比，脾虚小鼠在感染白念珠菌后生存天数明显缩短，肾脏内白念珠菌活菌菌数明显增多；脾虚小鼠体内抗体生成减少，单核吞噬细胞吞噬功能下降；白念珠菌能够抑制正常小鼠的免疫功能，表现为体内抗体生成减少，单核吞噬细胞吞噬功能下降，使小鼠抵抗力降低。
结论
脾虚小鼠对白念珠菌易感性增加，免疫力减弱，这与脾虚小鼠体内抗体生成减少，单核吞噬细胞吞噬功能下降有关。
Objective
In order to observe the response of spleen deficiency mice to intravenous infection of C.albicans.
Methods
The mice were fed with senna leaf decoction for seven days until the mice appeared some symptoms, such as diarrhea, bad appetite, emaciation and inactivity. Then the mice were injected C.albicans intravenously. The survival days of mice were observed for three weeks and the amount of live C.albicans in kidneys was calculated. The carbon particle clearance test and serum concentration of hemolysin were determined.
Results
The results suggest that C.albicans can suppress the immune function of the normal mice significantly. The phagocytosis of macrophages and the production of antibodies fell obviously. The survival days of senna-treated mice were sharply shorted after the infection of C.albicans and the amount of C.albicans in kidneys was increased. In contrast, the survival days of the normal mice were not different after the infection of C.albicans.
Conclusion
It means that C.albicans is lethal to spleen deficiency mice.
引文
[1] 陆德源主编．医学微生物学．人民卫生出版社．1997：208-209
[2] 滕维亚，狄惠芝．深部真菌感染的研究进展．中华医院感染学杂志．1999；9(1)：62
[3] 徐学瑛，等．一个改进的体液免疫测定方法——溶血素测定法．药学学报．1979，14(7)：443
[4] 陈奇主编．中药药理研究方法学．人民卫生出版社．1993：757-759
[5] Bistoni F, et al. Evidence for macrophage-mediated, protection against lethal Candida albicans infection.Infect Immun 1986; 51(2): 668
[6] Rodriguez-Galan MC, et al.Impaired activity of phagocytic cells in Candida albicans infection after exposure to chronic varied stress:Neuroimmunomodulation 2001;9(4):193
[7] Rodriguez-Galan MC,et al.Phenotypic and functional changes on phagocytic cells recruited at the site of Candida albicans infection after chronic varied stress exposure.Med Mycol 2002; 40(5):485.
[8] Netea MG, et al. CD40/CD40 ligand interactions in the host defense against disseminated Candida albicans infection:the role of macrophage-derived nitric oxide.Eur J Immunol 2002; 32(5):1455
[9] Lee SJ, et al. Normal host defense during systemic candidiasis in mannose receptor-deficient mice. Infect Immun 2003;71(1): 437
[10] Kedzierska K, et al.Defective phagocytosis by human monocyte/macrophages following HIV-1 infection:underlying mechanisms and modulation by adjunctive cytokine therapy. J Clin Virol 2003; 26(2): 247
[11] Han Y, et al. Candida albicans mannan extract-protein conjugates induce a protective immune response against experimental candidiasis.J Infect Dis 1999;179(6): 1477
[12] Han Y, et al. Complement is essential for protection by an IgM and an IgG3 monoclonal
antibody against experimental,hematogenously disseminated candidiasis.J Immunol 2001; 167(3):1550
[13] 王鲁．抗白念珠菌保护性抗体免疫．国外医学皮肤性病学分册．1999；25(4)：204
[14] 陈家旭，杨维益．中医脾虚证研究的进展．中国中医基础医学杂志．1995；1(3)：59
[15] 樊雅莉，陈小野．近10年脾虚证与免疫系统关系研究进展．中国中医药信息杂志．2001；8(4)：25
[16] 王运平，等．脾虚小鼠免疫功能的探讨．中国中西医结合脾胃杂志．1998；6(1)：34
[17] 吴玲霓，等．肾虚、脾虚造型动物免疫超微结构的比较研究．中医药研究．1999；15(3)：39
[18] 张洪泉，等．健脾消食冲剂对大黄脾虚模型小鼠的免疫功能影响．中国中西医结合脾胃杂志．1996；4(1)：42
[19] 许得盛，等．右归饮、四君子汤、桃红四物汤调节肾虚、脾虚、血瘀证患者免疫功能的观察．中国中西医结合杂志．1999；19(12)：712
[20] 吴敏毓，等．补中益气汤拆方组份对脾虚小鼠免疫调节作用．中国临床药理学与治疗学杂志．1997；2(2)：114
[21] 曲长江，等．不同造模方法脾虚小鼠免疫学改变的比较研究．中国中医基础医学杂志．1999；5(4)：46
[22] 章梅，等．四君子汤对脾虚患者血浆细胞因子的影响．第四军医大学学报．2000；21(4)：411
[23] 金敬善．脾虚证动物模型的实验研究进展．中国中西医结合脾胃杂志．1999；7(2)：65
[24] 南京医学院中西医结合研究组．脾虚泄泻证动物模型的研究．浙江中医杂志．1982；17(8)：355
[25] 沈自尹．中医虚证辨证参考标准．中西医结合杂志．1983；3(2)：117
[26] 北京师范大学生物系消化生理科研组．中医脾虚证动物模型的造型．中华医学杂志．1980；60(2)：83
[27] 刘汶，等．番泻叶致脾虚证动物模型的造型方法．中国中西医结合脾胃杂志．1998；6(4)：231
[28] 龙振洲主编．医学免疫学．人民卫生出版社．1996：16-18
[2] 滕维亚，狄惠芝．深部真菌感染的研究进展．中华医院感染学杂志．1999；9(1)：62
[3] 徐学瑛，等．一个改进的体液免疫测定方法——溶血素测定法．药学学报．1979，14(7)：443
[4] 陈奇主编．中药药理研究方法学．人民卫生出版社．1993：757-759
[5] Bistoni F, et al. Evidence for macrophage-mediated, protection against lethal Candida albicans infection.Infect Immun 1986; 51(2): 668
[6] Rodriguez-Galan MC, et al.Impaired activity of phagocytic cells in Candida albicans infection after exposure to chronic varied stress:Neuroimmunomodulation 2001;9(4):193
[7] Rodriguez-Galan MC,et al.Phenotypic and functional changes on phagocytic cells recruited at the site of Candida albicans infection after chronic varied stress exposure.Med Mycol 2002; 40(5):485.
[8] Netea MG, et al. CD40/CD40 ligand interactions in the host defense against disseminated Candida albicans infection:the role of macrophage-derived nitric oxide.Eur J Immunol 2002; 32(5):1455
[9] Lee SJ, et al. Normal host defense during systemic candidiasis in mannose receptor-deficient mice. Infect Immun 2003;71(1): 437
[10] Kedzierska K, et al.Defective phagocytosis by human monocyte/macrophages following HIV-1 infection:underlying mechanisms and modulation by adjunctive cytokine therapy. J Clin Virol 2003; 26(2): 247
[11] Han Y, et al. Candida albicans mannan extract-protein conjugates induce a protective immune response against experimental candidiasis.J Infect Dis 1999;179(6): 1477
[12] Han Y, et al. Complement is essential for protection by an IgM and an IgG3 monoclonal
antibody against experimental,hematogenously disseminated candidiasis.J Immunol 2001; 167(3):1550
[13] 王鲁．抗白念珠菌保护性抗体免疫．国外医学皮肤性病学分册．1999；25(4)：204
[14] 陈家旭，杨维益．中医脾虚证研究的进展．中国中医基础医学杂志．1995；1(3)：59
[15] 樊雅莉，陈小野．近10年脾虚证与免疫系统关系研究进展．中国中医药信息杂志．2001；8(4)：25
[16] 王运平，等．脾虚小鼠免疫功能的探讨．中国中西医结合脾胃杂志．1998；6(1)：34
[17] 吴玲霓，等．肾虚、脾虚造型动物免疫超微结构的比较研究．中医药研究．1999；15(3)：39
[18] 张洪泉，等．健脾消食冲剂对大黄脾虚模型小鼠的免疫功能影响．中国中西医结合脾胃杂志．1996；4(1)：42
[19] 许得盛，等．右归饮、四君子汤、桃红四物汤调节肾虚、脾虚、血瘀证患者免疫功能的观察．中国中西医结合杂志．1999；19(12)：712
[20] 吴敏毓，等．补中益气汤拆方组份对脾虚小鼠免疫调节作用．中国临床药理学与治疗学杂志．1997；2(2)：114
[21] 曲长江，等．不同造模方法脾虚小鼠免疫学改变的比较研究．中国中医基础医学杂志．1999；5(4)：46
[22] 章梅，等．四君子汤对脾虚患者血浆细胞因子的影响．第四军医大学学报．2000；21(4)：411
[23] 金敬善．脾虚证动物模型的实验研究进展．中国中西医结合脾胃杂志．1999；7(2)：65
[24] 南京医学院中西医结合研究组．脾虚泄泻证动物模型的研究．浙江中医杂志．1982；17(8)：355
[25] 沈自尹．中医虚证辨证参考标准．中西医结合杂志．1983；3(2)：117
[26] 北京师范大学生物系消化生理科研组．中医脾虚证动物模型的造型．中华医学杂志．1980；60(2)：83
[27] 刘汶，等．番泻叶致脾虚证动物模型的造型方法．中国中西医结合脾胃杂志．1998；6(4)：231
[28] 龙振洲主编．医学免疫学．人民卫生出版社．1996：16-18