FSH、LH受体肽疫苗对绒山羊公羊雄激素分泌及部分性状的影响
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摘要
本实验分别用促卵泡素受体肽疫苗(FSHR)和促黄体素受体肽疫苗(LHR)及其混合疫苗，分三种疫苗组合(实验1组RNA、SKV、SDL、GAL；2组RNA、SKV、SDL；3组GAL、GLAT)对内蒙古绒山羊公羊进行注射免疫。实验分对照组8只、实验1组12只、2组12只、3组8只共4组，从1月龄开始至12月龄，每45天为一周期将疫苗进行股部及体两侧三点皮下注射(对照组注射等量PBS)。同时采集实验羊的血清，用ELISA(酶联免疫测定)方法测定血清的抗体水平，用RIA(放射性免疫)方法测定各实验组羊血清雄激素水平；同时在实验中对羊的体重、体高、体长、胸围、管围、角长、角围、绒厚、抓绒量等性状进行了测定和观察。结果表明免疫的三个实验组羊的抗体水平明显高于对照组。G1、G2组公羊的雄激素分泌水平，在性成熟期与对照组差异不显著(P＞0.05)；实验3组的雄激素分泌水平在性成熟期明显低于对照组(P＜0.05)，说明实验3组的肽疫苗在性成熟时达到了抑制公羊雄激素分泌的目的。对部分性状的测定结果表明实验组与对照组差异不显著(P＞0.05)，说明疫苗注射对山羊的生长发育无不良影响。并且实验3组羊的平均体重比同龄羯羊平均体重高4.5公斤，其饲料转化率也高于同龄羯羊。对公羊的行为观察发现，实验3组公羊的爬跨次数明显低于对照组，而且其行为比其它组温和，易于管理。
In this study, 40 male cashmere goats were divided randomly into four groups (groupl(Gl)-12, group2(G2)- 12, group3(G3)- 8 and control Group- 8). During the period between Imonth age and 12 month age, the treatment groups of Inner Mongolia male cashmere goat were injected intradermally at three sites on the back skin and midside skin with different receptor peptides vaccine respectively (Gl RNA, SKV > SDL, GAL, G2 RNA, SKV, SDL, G3 GAL, GLAT) at 45days intervals and the control group was injected with PBS simultaneously. At the same time, all the animals were bled and the serum anti-body concentrations were tested with ELISA, the serum testosterone were determined with RIA, The results indicated that the serum concentrations of the corresponding anti-body of the three vaccinated groups were significantly higher than those of the control group. The serum testosterone concentration in Gl and G2 were not significantly(p>0.05) different from that of control group, while the animals at 7 month age in G3 the serum testosterone concentration was significantly(p<0.05)lower than that of control group, which implied the receptor peptide vaccine could inhibit the secretion of testosterone during the sexually mature in this group . Also, the body weight, cashmere length, cashmere weight in all treatment group were determined, and the results indicated that there were no significant different(p>0.05) among Gl, G2, G3 and control group, which implied that there was no negative effect of vaccine injection on the performance of cashmere goat. The results also indicate that the body weight and feed conversion ratio in G3 was 4.5 kg heavier and higher than those of the castrated animals at the same age. During the period
of observing the behavior of all the cashmere goat ,the sexual behavior of the animals in G3 were significantly lesser than that of control group and the agonistic behavior of the animals in G3 were correspondingly weaker than that of other groups, which indicated the animals in G3 was easy to be managed .
In this study, 40 male cashmere goats were divided randomly into four groups (groupl(Gl)-12, group2(G2)- 12, group3(G3)- 8 and control Group- 8). During the period between Imonth age and 12 month age, the treatment groups of Inner Mongolia male cashmere goat were injected intradermally at three sites on the back skin and midside skin with different receptor peptides vaccine respectively (Gl RNA, SKV > SDL, GAL, G2 RNA, SKV, SDL, G3 GAL, GLAT) at 45days intervals and the control group was injected with PBS simultaneously. At the same time, all the animals were bled and the serum anti-body concentrations were tested with ELISA, the serum testosterone were determined with RIA, The results indicated that the serum concentrations of the corresponding anti-body of the three vaccinated groups were significantly higher than those of the control group. The serum testosterone concentration in Gl and G2 were not significantly(p>0.05) different from that of control group, while the animals at 7 month age in G3 the serum testosterone concentration was significantly(p<0.05)lower than that of control group, which implied the receptor peptide vaccine could inhibit the secretion of testosterone during the sexually mature in this group . Also, the body weight, cashmere length, cashmere weight in all treatment group were determined, and the results indicated that there were no significant different(p>0.05) among Gl, G2, G3 and control group, which implied that there was no negative effect of vaccine injection on the performance of cashmere goat. The results also indicate that the body weight and feed conversion ratio in G3 was 4.5 kg heavier and higher than those of the castrated animals at the same age. During the period
of observing the behavior of all the cashmere goat ,the sexual behavior of the animals in G3 were significantly lesser than that of control group and the agonistic behavior of the animals in G3 were correspondingly weaker than that of other groups, which indicated the animals in G3 was easy to be managed .
引文
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2 张家峥．公畜无血去势机理研究．中国兽医科技，1986，(4)：25
3 贾继祥．牛提睾术的应用实验．黑龙江畜牧兽医，1984，(3)：24
4 彭连生．使用结扎法去势公马的体会．中国兽医杂志，1988，(2)：34
5 夏道伦．公畜药物去势法．农业科技通讯，1990，(3)：24
6 张云卿．药物去势术的观察和思考．中国兽医科技，1987，(8)：52
7 冯大刚．新的小公牛去势法-化学去势．甘肃畜牧兽医，1986，(2)：43
8 庞学钧．用超低温液氮冷冻去势法去势公鸡．中国兽医杂志，1985，(1)：32
9 蔡含筠．扎骟法在公羔育肥的应用．畜牧兽医杂志，1986，(1)：42
10 刘权．仔公猪免疫去势研究．黑龙江畜牧兽医杂志，1984，(3)：24
11 张得福．激素免疫技术在畜牧业生产上的应用．畜牧杂志，1993：30-32．
12 岳海宁，吴润．GnRH主动免疫对绵羊血清睾酮含量的影响．甘肃农业大学学报，1995，30(2)：156-159
13 Jean-Jacques Remy, et al. Immunization against exon 1 decapepitide from the lutropin/choriogonadotropin receptor or the follitropin receptor as potential male contraceptive. Journal of Reproductve Immunology, 1996, 32:37-54
14 赵伟．公猪免疫去势法初报．畜牧与兽医，1992，24(5)：197-198
15 岳海宁．LHRH主动免疫对绵羊血清LH浓度的影响．中国兽医杂志，1996，22(1)：12-13．
16 Fraster, H.M.and Gunn,A,. Effects of antibodies to luteinizing hormone releasing hormone in the male rabbit and on the rat oestrous cycle. Nature, 1983, 224:160
17 Fraster, H.M. et al. Effects of active immunization to luteinizing hormone releasing hormone on serum and pituitary gonadotrophins,tests and accessory sex organs in the male rat. Journal of Endocrinology, 1974, 63:399
18 Chappel,S.C. et al. active immunization of male rhesus monkeys against luteinizing hormone releasing hormone. Biol. Reprod, 1980, 22:333
19 Robbertson, I.S. et al. Immunological castration in male cattle. Vet. Rec, 1979, 105:556
20 刘学高．国产避孕疫苗研究现状和前景．动物学研究，2000，21(1)：23-27
21 朱培元等．支持细胞功能和分化的激素调控．中华男科学，2001，7(1)：48-51
22 Bhowmick N,Huang JN, et al. Determination of residues important in hormone binding to the extracellular domain of the lutropin/choriogonadotropin receptor by site-directed mutagenesis and modeling. Molecular Endocrinology, 1996,10:1147-1159
23 Mclachlan R,Wreford N, et al. Hormone control of spermatogenesis. Trends in Endocrinology and Metabolism, 1995, 6:95-101
24 高瑞娟．睾丸间质细胞睾酮合成的局部调节，国外医学计划生育分册，2000，19(2)：65-68．
25 Hanumanthappa Krishnamurthy, Rouslan Kats,et al. Intercellular Communication between Sertoli cells and Leydig Cells in the Absence of Follicle-Stimulating Hormone-Receptor Signaling. BIOLOGY OF REPRODUCTION, 2001 65:1201-1207
26 梁小兵，周洁．卵泡刺激素受体主动免疫对雄性小鼠生育力影响的初步研究．生殖医学杂志，2000，19(8)：230-233
27 Ranniki AS.Zhang FP. et al. Ontogeny of follicle-stimulating hormone receptor gene expression in the rat testis and ovary. Mol Cell Endocrinol, 1995, 107:199-208
28 陆金春 LH／CG受体的研究进展．<生殖健康与计划生育>研讨会部分论文摘要．2000．56-57
29 Kajava. A.V. Vassart, G. et al. Modeling of the three-dimensional structure of proteins with the typical leucine-rich repeats. Structure, 1995, 3:867-877
30 Salesse, R. and Garnier, J. Glycoprotein hormone receptors: new molecular supports for old biological functions. Biomembranes, 1996 2
31 Roche, P, C. Ryan, R.J. et al. Identification of hormone binding regions of the luteinizing hormone/human chorionic gonadotropin receptor using synthetic peptides. Endocrinology, 1992, 131:268-274
32 Morris, J.C.Ⅲ, Berger E.R. et al. Structure-function studies of the human thyrotropin receptor. Inhibition of binding labeled thyrotropin by synthetic human TSH receptor peptides. Biol. Chem, 1993,270:20020-20031
33 L Abdennedi.L Couture. Generating FSH antagonists and agonists through immunization against FSH receptor N-terminal decapeptides. Journal of Molecular Endocrinology, 1999, 22:1-9
34 甄万清．奶公牛去势对育肥的影响．黑龙江畜牧兽医杂志，1993，(10)：17
35 杨帆等．雄激素对小鼠免疫功能的影响．中国免疫学杂志，1998，4(3)：340-342
36 L Couture,H naharisoa,D Grebert, et al. Peptide and immunochemical mapping of the ectodomain of the porcine LH receptor. Jounal of Molecular Endocrinology, 1996, 16:15-25
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