不同湖泊瓦氏雅罗鱼血液指标和同工酶的研究
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摘要
瓦氏雅罗鱼为达里诺尔湖的主产经济鱼类,具有很强的耐盐碱性,本文主要对生活于半咸水水域(达里诺尔湖)和淡水水域(岗更湖)中的瓦氏雅罗鱼的血液生化指标、Na+,K+—ATP酶活性、同工酶进行了比较研究,旨在为探索瓦氏雅罗鱼耐盐碱机理做基础性的研究,同时也为瓦氏雅罗鱼的引种、放流和人工养殖方面的研究提供科学依据。
本论文主要包括三部分的研究:
(1)第一部分的试验主要研究了瓦氏雅罗鱼的血液生化指标,研究结果得出:两种水域中瓦氏雅罗鱼的白蛋白、白蛋白/球蛋白、磷酸根离子、尿酸、钾、氯、钠、钙、镁、甘油三酯等生化指标差异极显著(P<0.01);总蛋白差异不显著(P>0.05)。
(2)第二部分的试验主要研究了瓦氏雅罗鱼的Na+,K+—ATP酶活性,研究结果得出:达里诺尔湖瓦氏雅罗鱼鳃丝组织的Na+,K+—ATP酶活性显著高于岗更湖瓦氏雅罗鱼鳃丝组织Na+,K+—ATP酶活性,而两湖中瓦氏雅罗鱼肌肉和肝脏组织中Na+,K+—ATP酶活性差异不显著;同时试验结果也表明同一水域中瓦氏雅罗鱼的鳃丝组织的Na+,K+—ATP酶活性显著高于肌肉和肝脏组织的Na+,K+—ATP酶活性。
(3)第三部分的试验主要运用聚丙烯酰胺凝胶电泳技术研究了两种水域中瓦氏雅罗鱼的骨骼肌、肝脏、心肌组织中过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)同工酶,研究结果得出:两湖中瓦氏雅罗鱼的过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)等同工酶酶带和酶的活性均有差异性,而苹果酸脱氢酶(MDH)同工酶差异不显著。
Leuciscus waleckii is main commercial fish in Dalinuoer lake,which can undertake salinity strongly, in this paper comparative studied blood biochemical parameters、Na+,K+—ATP enzyme activity and isozyme of Leuciscus waleckii live in salt water(Dalinor-Lake) and fresh waters(ganggeng-lake). Aims to explore the mechanism of Leuciscus waleckii salinity to do basic research,and also provides scientific basis for the Leuciscus waleckii introduction, releasing, and cultured.
This paper mainly includes three parts of the study:
(1) The first part of the experiment mainly studies the Leuciscus waleckii’s blood biochemical parameters, the results obtained: Two kinds of waters Leuciscus waleckii’s albumin, albumin / globulin, phosphate ions, uric acid, potassium, chloride, sodium, calcium, magnesium, triglycerides and other biochemical indicators are significant difference (P<0.01); no significant difference in total protein(P>0.05).
(2) The second part of the experiment mainly studies the Leuciscus waleckii’s Na+,K+—ATP enzyme activity, the results obtained: Leuciscus waleckii gill tissue’s Na+,K+—ATP enzyme activity in Dalinuoer Lake was significantly higher than Leuciscus waleckii in ganggeng-lake,while Leuciscus waleckii muscle and liver tissue’s Na+,K+—ATP enzyme activity in the tow lakes was no significant difference; And the same time, test results also show that Leuciscus waleckii gill tissue’s Na+,K+—ATP enzyme activity was significantly higher than muscle and liver tissue’s Na+,K+—ATP enzyme activity in the same waters.
(3) The third part of the experiment mainly comparatively studies the Isoenzymes of Leuciscus waleckii live in salt water and fresh waters with polyacrylamidegel electrophoresis (PAGE).Through a comparative study peroxidase (POD), esterase (EST), lactate dehydrogenase (LDH)and malic acid de-hydrogenase (MDH) in muscle, liver and heart tissue of Leuciscus waleckii, the results obtained: Leuciscus waleckii with differences in expression of the isozymes in the two lakes, and EST mapping has the biggest differences between them, LDH and POD are also have differences in the pattern, but MDH pattern no significant difference.
本论文主要包括三部分的研究:
(1)第一部分的试验主要研究了瓦氏雅罗鱼的血液生化指标,研究结果得出:两种水域中瓦氏雅罗鱼的白蛋白、白蛋白/球蛋白、磷酸根离子、尿酸、钾、氯、钠、钙、镁、甘油三酯等生化指标差异极显著(P<0.01);总蛋白差异不显著(P>0.05)。
(2)第二部分的试验主要研究了瓦氏雅罗鱼的Na+,K+—ATP酶活性,研究结果得出:达里诺尔湖瓦氏雅罗鱼鳃丝组织的Na+,K+—ATP酶活性显著高于岗更湖瓦氏雅罗鱼鳃丝组织Na+,K+—ATP酶活性,而两湖中瓦氏雅罗鱼肌肉和肝脏组织中Na+,K+—ATP酶活性差异不显著;同时试验结果也表明同一水域中瓦氏雅罗鱼的鳃丝组织的Na+,K+—ATP酶活性显著高于肌肉和肝脏组织的Na+,K+—ATP酶活性。
(3)第三部分的试验主要运用聚丙烯酰胺凝胶电泳技术研究了两种水域中瓦氏雅罗鱼的骨骼肌、肝脏、心肌组织中过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)同工酶,研究结果得出:两湖中瓦氏雅罗鱼的过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)等同工酶酶带和酶的活性均有差异性,而苹果酸脱氢酶(MDH)同工酶差异不显著。
Leuciscus waleckii is main commercial fish in Dalinuoer lake,which can undertake salinity strongly, in this paper comparative studied blood biochemical parameters、Na+,K+—ATP enzyme activity and isozyme of Leuciscus waleckii live in salt water(Dalinor-Lake) and fresh waters(ganggeng-lake). Aims to explore the mechanism of Leuciscus waleckii salinity to do basic research,and also provides scientific basis for the Leuciscus waleckii introduction, releasing, and cultured.
This paper mainly includes three parts of the study:
(1) The first part of the experiment mainly studies the Leuciscus waleckii’s blood biochemical parameters, the results obtained: Two kinds of waters Leuciscus waleckii’s albumin, albumin / globulin, phosphate ions, uric acid, potassium, chloride, sodium, calcium, magnesium, triglycerides and other biochemical indicators are significant difference (P<0.01); no significant difference in total protein(P>0.05).
(2) The second part of the experiment mainly studies the Leuciscus waleckii’s Na+,K+—ATP enzyme activity, the results obtained: Leuciscus waleckii gill tissue’s Na+,K+—ATP enzyme activity in Dalinuoer Lake was significantly higher than Leuciscus waleckii in ganggeng-lake,while Leuciscus waleckii muscle and liver tissue’s Na+,K+—ATP enzyme activity in the tow lakes was no significant difference; And the same time, test results also show that Leuciscus waleckii gill tissue’s Na+,K+—ATP enzyme activity was significantly higher than muscle and liver tissue’s Na+,K+—ATP enzyme activity in the same waters.
(3) The third part of the experiment mainly comparatively studies the Isoenzymes of Leuciscus waleckii live in salt water and fresh waters with polyacrylamidegel electrophoresis (PAGE).Through a comparative study peroxidase (POD), esterase (EST), lactate dehydrogenase (LDH)and malic acid de-hydrogenase (MDH) in muscle, liver and heart tissue of Leuciscus waleckii, the results obtained: Leuciscus waleckii with differences in expression of the isozymes in the two lakes, and EST mapping has the biggest differences between them, LDH and POD are also have differences in the pattern, but MDH pattern no significant difference.
引文
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