抵当芪桂汤对SD大鼠糖尿病性白内障作用的实验研究
摘要
目的:本实验旨在通过抵当芪桂汤对SD大鼠糖尿病性白内障的实验研究,观察实验性糖尿病大鼠晶状体的变化、晶状体上皮细胞的改变、以及HSP70的水平,探讨抵当芪桂汤对糖尿病大鼠白内障的发生发展是否具有抑制作用,为今后在临床上进一步探讨抵当芪桂汤对糖尿病性白内障患者的防治提供参考。
方法:大鼠购回后随机选取10只作为空白对照组(A组),用腹腔注射等量柠檬酸缓冲液,其余70只大鼠在禁食12h后后一次性腹腔注射链脲佐菌素(STZ)60mg/kg,注射后72小时后测其血糖值≥16.9mmol/L,尿糖+++～+++以上者为造模成功。剔除14只造模未成功的大鼠,将造模成功的56只大鼠随机分为5组:模型对照组(简称B组,12只)、达美康对照组(简称C组,11只)、抵当芪桂汤正常剂量组(简称D组,11只)、抵当芪桂汤大剂量组(简称E组,11只)、抵当芪桂汤加达美康组(简称F组,11只),并对各组大鼠给予相应干预。在实验2、4、6、8周,0.5%托品酰胺滴眼液散瞳,在裂隙灯下检查晶体混浊情况,并记录混浊情况;给药8周后处死前取血测定血糖,10%水合氯醛麻醉过量致死,迅速取出眼球并用4%多聚甲醛固定液固定后,制成0.4μm厚冰冻切片,经苏木精-伊红(HE)染色切片,于光学显微镜下观察、摄片,观察晶状体上皮细胞形态变化;并用免疫组化SP二步法检测晶状体上皮细胞(LEC)中的HSP70表达情况。
结果:A组大鼠晶状体始终保持透明完整,B组大鼠晶状体逐渐以致完全混浊,其余治疗组间晶状体发生不同程度的混浊,晶体混浊程度由重到轻依次为E组>D组≥C组≥F组>A组;HE染色晶状体上皮细胞形态亦发生不同改变;以A组大鼠作为标准, B组晶状体L EC中HSP_(70)表达逐渐增多,治疗组E组HSP70的阳性表达率高于C组、D组、F组HSP70的阳性表达率,差异具有显著性(P < 0.05),C组、D组、F组间HSP_(70)的阳性表达率无显著性差异(P >0.05)。
结论:抵当芪桂汤能够有效保护实验性糖尿病大鼠晶状体损伤,抑制糖尿病大鼠白内障的发生和发展,对早期糖尿病大鼠白内障具有一定的防治作用,其机制可能与抵当芪桂汤降低血糖、减少糖代谢产物在晶状体内聚集、降低晶状体内渗透压,从而减轻晶状体代谢损伤有关。
Objective: To investigate the inhibitory effect of Didangqigui Soup on the occurrence and development of SD rats’diabetic cataract, and provide clinical reference for its prevention and treatment of diabetic cataract.
Methods: Rats were randomly selected as control group (group A), with intraperitoneal injection amount citric acid buffer A, the other 70 rats were given chain meal (STZ 60mg/kg) to make diabetic model after fasting 12 hours,72 hours later measured their blood sugar .The blood sugar level which≥16.9mmol/L, urine sugar with more than +++～+++,were both regarded as made mould success. 14 rats were removed, the remaining 56 randomly divided into 5 groups respectively: model control group (group B), western medicine group (group C), Chinese traditional medicine normal dosage group (D group), Chinese traditional medicine high-dose group (group E), Chinese medicine with western medicine group (normal doses of group F).Then gave corresponding intervention. Then 0.5% tetramin methenamine amide eye drops are used as mydriatic in week 2, 4, 6 and 8.The condition of lens opacity were examined and recorded in the slit lamp. Eight weeks later ,blood glucose were determined before the determination of death by 10% hydrated chlorine aldehyde anesthesia fatal overdose. Then eyes were quickly removed, fixed by more than 4% after fixation gather formaldehyde and made frozen section as 0.4μm thick. After HE dyed,the change of the lens epithelial cells wer e observed under optical microcopy . And the expression of HSP_(70) in LEC was detected by immunohistochemical SP ldpe-g-nvp.
Results: Rat lens of Group A always maintain transparent completely, rats lens of group B gradually opacity. Compared with group A, each group lens happened different degree of opacity, crystal determinated by heavy to light in turn for group E >group D≥group C≥group F >group A; HE dyeing lens epithelial cells form also change. The expression of HSP_(70) in LEC in group A was standard,which in group B increased gradually. That is higher than group C and D group. The expression of HSP_(70) in LEC in group F with significant difference (P < 0.05), group C, D group, the group F HSP_(70) positive expression rate between non-significance difference (P > 0.05).
Conclusion: Didangqigui Soup could effectively protect the damage of experimental diabetic rats lens, inhibit the occurrence and developmen of diabetic rats catarac,especially in the early disease development. The mechanism may be that:it could lower blood sugar, reduce sugar metabolites gathered inside lens ,and lower osmotic pressure inside lens, so that lessen the lens on the metabolic damage.
方法:大鼠购回后随机选取10只作为空白对照组(A组),用腹腔注射等量柠檬酸缓冲液,其余70只大鼠在禁食12h后后一次性腹腔注射链脲佐菌素(STZ)60mg/kg,注射后72小时后测其血糖值≥16.9mmol/L,尿糖+++～+++以上者为造模成功。剔除14只造模未成功的大鼠,将造模成功的56只大鼠随机分为5组:模型对照组(简称B组,12只)、达美康对照组(简称C组,11只)、抵当芪桂汤正常剂量组(简称D组,11只)、抵当芪桂汤大剂量组(简称E组,11只)、抵当芪桂汤加达美康组(简称F组,11只),并对各组大鼠给予相应干预。在实验2、4、6、8周,0.5%托品酰胺滴眼液散瞳,在裂隙灯下检查晶体混浊情况,并记录混浊情况;给药8周后处死前取血测定血糖,10%水合氯醛麻醉过量致死,迅速取出眼球并用4%多聚甲醛固定液固定后,制成0.4μm厚冰冻切片,经苏木精-伊红(HE)染色切片,于光学显微镜下观察、摄片,观察晶状体上皮细胞形态变化;并用免疫组化SP二步法检测晶状体上皮细胞(LEC)中的HSP70表达情况。
结果:A组大鼠晶状体始终保持透明完整,B组大鼠晶状体逐渐以致完全混浊,其余治疗组间晶状体发生不同程度的混浊,晶体混浊程度由重到轻依次为E组>D组≥C组≥F组>A组;HE染色晶状体上皮细胞形态亦发生不同改变;以A组大鼠作为标准, B组晶状体L EC中HSP_(70)表达逐渐增多,治疗组E组HSP70的阳性表达率高于C组、D组、F组HSP70的阳性表达率,差异具有显著性(P < 0.05),C组、D组、F组间HSP_(70)的阳性表达率无显著性差异(P >0.05)。
结论:抵当芪桂汤能够有效保护实验性糖尿病大鼠晶状体损伤,抑制糖尿病大鼠白内障的发生和发展,对早期糖尿病大鼠白内障具有一定的防治作用,其机制可能与抵当芪桂汤降低血糖、减少糖代谢产物在晶状体内聚集、降低晶状体内渗透压,从而减轻晶状体代谢损伤有关。
Objective: To investigate the inhibitory effect of Didangqigui Soup on the occurrence and development of SD rats’diabetic cataract, and provide clinical reference for its prevention and treatment of diabetic cataract.
Methods: Rats were randomly selected as control group (group A), with intraperitoneal injection amount citric acid buffer A, the other 70 rats were given chain meal (STZ 60mg/kg) to make diabetic model after fasting 12 hours,72 hours later measured their blood sugar .The blood sugar level which≥16.9mmol/L, urine sugar with more than +++～+++,were both regarded as made mould success. 14 rats were removed, the remaining 56 randomly divided into 5 groups respectively: model control group (group B), western medicine group (group C), Chinese traditional medicine normal dosage group (D group), Chinese traditional medicine high-dose group (group E), Chinese medicine with western medicine group (normal doses of group F).Then gave corresponding intervention. Then 0.5% tetramin methenamine amide eye drops are used as mydriatic in week 2, 4, 6 and 8.The condition of lens opacity were examined and recorded in the slit lamp. Eight weeks later ,blood glucose were determined before the determination of death by 10% hydrated chlorine aldehyde anesthesia fatal overdose. Then eyes were quickly removed, fixed by more than 4% after fixation gather formaldehyde and made frozen section as 0.4μm thick. After HE dyed,the change of the lens epithelial cells wer e observed under optical microcopy . And the expression of HSP_(70) in LEC was detected by immunohistochemical SP ldpe-g-nvp.
Results: Rat lens of Group A always maintain transparent completely, rats lens of group B gradually opacity. Compared with group A, each group lens happened different degree of opacity, crystal determinated by heavy to light in turn for group E >group D≥group C≥group F >group A; HE dyeing lens epithelial cells form also change. The expression of HSP_(70) in LEC in group A was standard,which in group B increased gradually. That is higher than group C and D group. The expression of HSP_(70) in LEC in group F with significant difference (P < 0.05), group C, D group, the group F HSP_(70) positive expression rate between non-significance difference (P > 0.05).
Conclusion: Didangqigui Soup could effectively protect the damage of experimental diabetic rats lens, inhibit the occurrence and developmen of diabetic rats catarac,especially in the early disease development. The mechanism may be that:it could lower blood sugar, reduce sugar metabolites gathered inside lens ,and lower osmotic pressure inside lens, so that lessen the lens on the metabolic damage.
引文
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