南江黄羊IGFs基因的时空表达研究
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摘要
IGFs具有促进生长发育、细胞的增殖和分化、物质代谢等生理功能。本试验选取0d、15d、30d、60d、90d、120d等6个时间点和半膜肌(BM)、臂三头肌(BS)、背最长肌(BZ)、股四头肌(GST)、臀股二头肌(TG)、肝脏、脾脏、心脏等8个组织,采用SYBR GreenⅠ荧光定量PCR技术研究IGFs基因在南江黄羊生后期的时空表达,其结果表明：
(1)除少数组织公母间表达量差异达极显著水平外,其余组织的性别间差异均不显著(P>0.05),表明性别对IGFs基因的表达量差异有一定影响,但影响不大。而差异极显著的组织主要是肝脏和脾脏,且IGFs在公羔中的表达量高于母羔,可能受营养、激素等因素影响。
(2) IGF-Ⅰ基因在6个时间点中都呈现肝脏表达量最高,其次是脾脏。另外,0d、15d和30d这三个时间点中BS表达量较高。30d时GST的表达量也较高,且与BS表达量差异不显著(P>0.05)。表明肝脏是IGF-Ⅰ蛋白分泌的重要来源。除肝脏外,其余组织也能够自分泌或旁分泌IGF-Ⅰ蛋白,且IGF-Ⅰ基因对脾脏发育具有重要的调节作用。
(3) IGF-Ⅱ基因在6个时间点中都呈现脾脏表达量最高,其次是肝脏,其余组织的表达量均较低。表明IGF-Ⅱ基因可能通过旁分泌或内分泌调控脾脏发育。
(4) IGF-Ⅰ基因在8个不同的组织中均呈现90d时表达最高。Od时较高,15d时有所下降。从15d开始到90d均上升,且上升趋势较为明显。90d到120d,出现下降趋势。所有组织在6个不同的时间点均出现上升趋势,只不过呈现波动性上升,表明IGF-I基因能够调控南江黄羊的生长发育。
(5) IGF-Ⅱ基因在8个不同的组织中均呈现60d时表达最高。0d时较高,15d时有所下降。从15d开始到60d均上升,且上升趋势较为明显。60d到120d,出现下降趋势。表明IGF-Ⅱ基因促进细胞的生长与分化的生物学功能更多的集中在60d,且对南江黄羊的生长发育具有调控作用。
IGFs played physiological roles on promoting growth, cell proliferation, differentiation and stimulating metabolism. Nanjiang Yellow Goats from 8 different tissues (semimembranosus (BM),triceps brachii muscle (BS), longissimus dorsi muscle (BZ), quadriceps femoris (GST),musculus biceps femoris buttocks (TG), liver, spleen and heart) and 6 different ages (0d, 15d,30d,60d,90d, 120d) were selected to study the spatialtemporal expression of IGFs genes by SYBR GreenⅠfluorescence quantitative PCR technique.The results were as follows:
(1) Most of the other tissues didn't have significant difference between male and female goats except a few tissues(P>0.05). It showed that the gender had a little effect on the expression of IGFs genes,but not big.The tissues which had extremely significant differences were mainly liver and spleen.The expression difference of IGFs genes between male and female lambs might be affected by nutrition, hormones and other factors.
(2) IGF-Ⅰgene expression quantity of the liver at different periods were the highest, following by the spleen.But for the muscle tissues, the expression quantity of BS was relatively higher at the three time points(0d,15d and 30d). The expression of GST was much higher at 30d,but it had not significant difference between GST and BS (P>0.05). The results indicated that the liver was an important source of protein secretion of IGF-Ⅰ.Besides the liver, the other tissues could also secret IGF-Ⅰprotein by autocrine or paracrine mechanisms,and IGF-Ⅰgene had an important regulation roles on the spleen development.
(3) IGF-Ⅱgene expression quantity of the spleen at different.periods were the highest, following by the liver,but the expression of other tissues were relatively lower.It showed that IGF-Ⅱgene regulated the development of spleen by paracrine or endocrine possibly.
(4) Among eight different tissuses, IGF-Ⅰgene expression quantity at the six different time points showed rising trend,but it was fluctuating rising. Expression quantity of IGF-Ⅰgene reached the highest at 90d. The rising trend from 15d to 90d was relative obviously,but it was declining from 90d to 120d.The result showed that IGF-Ⅰgene could regulate the growth of Nanjiang Yellow Goats.
(5) Among eight different tissuses, expression quantity of IGF-Ⅱgene at the six different time points also showed rising trend and it had the highest expression at 60d. The expression of IGF-Ⅱgene was rising from 15d to 60d,and the rising trend was relative obviously,but it was declining from 60d to 120d. These results showed that the biological functions of IGF-Ⅱgene were more concenteated at 60d,and it could regulate the growth and development of Nanjiang Yellow Goats.
(1)除少数组织公母间表达量差异达极显著水平外,其余组织的性别间差异均不显著(P>0.05),表明性别对IGFs基因的表达量差异有一定影响,但影响不大。而差异极显著的组织主要是肝脏和脾脏,且IGFs在公羔中的表达量高于母羔,可能受营养、激素等因素影响。
(2) IGF-Ⅰ基因在6个时间点中都呈现肝脏表达量最高,其次是脾脏。另外,0d、15d和30d这三个时间点中BS表达量较高。30d时GST的表达量也较高,且与BS表达量差异不显著(P>0.05)。表明肝脏是IGF-Ⅰ蛋白分泌的重要来源。除肝脏外,其余组织也能够自分泌或旁分泌IGF-Ⅰ蛋白,且IGF-Ⅰ基因对脾脏发育具有重要的调节作用。
(3) IGF-Ⅱ基因在6个时间点中都呈现脾脏表达量最高,其次是肝脏,其余组织的表达量均较低。表明IGF-Ⅱ基因可能通过旁分泌或内分泌调控脾脏发育。
(4) IGF-Ⅰ基因在8个不同的组织中均呈现90d时表达最高。Od时较高,15d时有所下降。从15d开始到90d均上升,且上升趋势较为明显。90d到120d,出现下降趋势。所有组织在6个不同的时间点均出现上升趋势,只不过呈现波动性上升,表明IGF-I基因能够调控南江黄羊的生长发育。
(5) IGF-Ⅱ基因在8个不同的组织中均呈现60d时表达最高。0d时较高,15d时有所下降。从15d开始到60d均上升,且上升趋势较为明显。60d到120d,出现下降趋势。表明IGF-Ⅱ基因促进细胞的生长与分化的生物学功能更多的集中在60d,且对南江黄羊的生长发育具有调控作用。
IGFs played physiological roles on promoting growth, cell proliferation, differentiation and stimulating metabolism. Nanjiang Yellow Goats from 8 different tissues (semimembranosus (BM),triceps brachii muscle (BS), longissimus dorsi muscle (BZ), quadriceps femoris (GST),musculus biceps femoris buttocks (TG), liver, spleen and heart) and 6 different ages (0d, 15d,30d,60d,90d, 120d) were selected to study the spatialtemporal expression of IGFs genes by SYBR GreenⅠfluorescence quantitative PCR technique.The results were as follows:
(1) Most of the other tissues didn't have significant difference between male and female goats except a few tissues(P>0.05). It showed that the gender had a little effect on the expression of IGFs genes,but not big.The tissues which had extremely significant differences were mainly liver and spleen.The expression difference of IGFs genes between male and female lambs might be affected by nutrition, hormones and other factors.
(2) IGF-Ⅰgene expression quantity of the liver at different periods were the highest, following by the spleen.But for the muscle tissues, the expression quantity of BS was relatively higher at the three time points(0d,15d and 30d). The expression of GST was much higher at 30d,but it had not significant difference between GST and BS (P>0.05). The results indicated that the liver was an important source of protein secretion of IGF-Ⅰ.Besides the liver, the other tissues could also secret IGF-Ⅰprotein by autocrine or paracrine mechanisms,and IGF-Ⅰgene had an important regulation roles on the spleen development.
(3) IGF-Ⅱgene expression quantity of the spleen at different.periods were the highest, following by the liver,but the expression of other tissues were relatively lower.It showed that IGF-Ⅱgene regulated the development of spleen by paracrine or endocrine possibly.
(4) Among eight different tissuses, IGF-Ⅰgene expression quantity at the six different time points showed rising trend,but it was fluctuating rising. Expression quantity of IGF-Ⅰgene reached the highest at 90d. The rising trend from 15d to 90d was relative obviously,but it was declining from 90d to 120d.The result showed that IGF-Ⅰgene could regulate the growth of Nanjiang Yellow Goats.
(5) Among eight different tissuses, expression quantity of IGF-Ⅱgene at the six different time points also showed rising trend and it had the highest expression at 60d. The expression of IGF-Ⅱgene was rising from 15d to 60d,and the rising trend was relative obviously,but it was declining from 60d to 120d. These results showed that the biological functions of IGF-Ⅱgene were more concenteated at 60d,and it could regulate the growth and development of Nanjiang Yellow Goats.
引文
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