鲜切生姜褐变机理及保鲜关键技术研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
鲜切果蔬具有新鲜、食用方便、营养安全、百分之百可食等多种优点,国内外市场前景广阔。生姜是具有特有保健功能的调味蔬菜之一,鲜切生姜加工技术易实施,生产成本较低,深入研究鲜切生姜在加工过程中褐变特性及控制技术,对阐明鲜切生姜褐变机理,提高鲜切生姜感官质量,促进其工业化生产具有理论和现实意义。本文以鲜切生姜为研究对象,采用高效液相色谱-电喷雾飞行时间质谱、透射电镜等方法,系统研究了鲜切生姜贮藏期间褐变度(BD)、总酚(TP)、多酚氧化酶(PPO)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活力的变化以及相关性,分离鉴定了鲜切生姜的褐变底物,明确了鲜切生姜的褐变机理,并在此研究基础上,对鲜切生姜的保鲜技术进行了研究。主要研究结果如下:
     (1)鲜切生姜在切割后PPO、POD、PAL活性迅速增强,PAL的活性增强促进了鲜切生姜中酚类物质生成,为PPO、POD提供了更多的底物,PPO、POD催化生姜中酚类物质的氧化是导致鲜切生姜褐变的主要原因。
     (2)从生姜薄层分离物中共分离鉴定出4-姜烯酚(4-shogaol)、1-脱氢-8-姜二酮(1-dehydro-8-gingerdione)、6-姜酚(6-gingerol)、8-姜烯酚(8-shogaol)、6-姜烯酚(6-shogaol)、8-姜二酮(8-gingerdione)、1-脱氢-6-姜二酮(1-dehydro-6-gingerdione)、10-姜烯酚(10-shogaol)八种含有3-甲氧基-4-羟基苯基官能团的褐变底物。
     (3)生姜PPO的最适反应温度为30℃,最适pH值为6.8,100℃处理2min可使PPO活性丧失;VC、NaHSO3、L-半胱氨酸、柠檬酸均对PPO有一定的抑制作用,其中L-半胱氨酸对生姜PPO抑制效果最好,其次为NaHSO3和VC;Ca~(2+)、Cu~(2+)对生姜PPO有激活作用,Al~(3+)和Mg~(2+)则对PPO表现为抑制作用。
     (4)生姜POD的最适反应温度为45℃,最适pH值为6.0,90℃处理60s或100℃处理40s可使POD活性丧失;VC、EDTA-2Na、NaHSO3、柠檬酸、L-半胱氨酸均对POD有一定的抑制作用,其中VC、NaHSO3、L-半胱氨酸抑制效果较好,EDTA-2Na、柠檬酸的抑制效果较差;Ca~(2+)、Mg~(2+)、Fe~(3+)、Zn~(2+)在5mmol/L时对POD均有激活作用,Na+、Mn~(2+)则有抑制作用。
     (5)生姜PAL的最适反应温度为60℃,最适pH值为8.2,生姜PAL耐热性很强,90℃处理15min或100℃处理10min方可使其完全失去活性;金属离子Mn~(2+)、Zn~(2+)、Cu~(2+)、Al~(3+)对PAL有激活作用,Ni~(2+)具有抑制作用。
     (6)壳聚糖涂膜可以减轻鲜切生姜贮藏过程中的褐变、质量损失,抑制可溶性总糖及VC的减少,抑制粗纤维的增加。质量分数1.5%的壳聚糖涂膜处理可以延缓鲜切生姜细胞质壁分离现象的发生,抑制细胞壁的降解以及细胞核、质体、线粒体的破坏和解体,保护细胞结构的完整性,延长鲜切生姜货架期。
     (7)鲜切生姜化学抑制剂最佳护色技术方案为0.3%VC+0.5%柠檬酸+0.4%L-半胱氨酸;壳聚糖涂膜最佳质量分数为1.5%。壳聚糖涂膜护色优于化学抑制剂护色。
Fresh-cut fruits and vegetables have exhibited a promising prospect in recent years dueto their potential advantages such as fresh, easy-to-serve and nutritional. Ginger possessessome health-promoting properties, and is widely used as one of traditional spices in China.Fresh-cut technology has the advantages of simple processing and low cost compared withother methods. In order to elucidate the browning mechanism of fresh-cut ginger and improvetheir sensory quality, it is essential to investigate the browning properties and control methodsof fresh-cut ginger. It is of great theoretical and realistic significance for industrialdevelopment of fresh-cut ginger. In the present study, browning degree (BD), totalphenols(TP), polyphenoloxidase(PPO), peroxidase(POD) and Phenylalanine ammonialyase(PAL) activity and their correlation were investigated during the storage of fresh-cutginger using high HPLC-ESI-TOF-MS and transmission electron microscope (TEM).Browning substrates of fresh-cut ginger were isolated and identified with the purpose ofunderstanding the browning mechanism of fresh-cut ginger. The preservation technology offresh-cut ginger was investigated as well. The results were as follows:
     (1) PPO, POD and PAL activities were rapidly elevated after cutting of fresh-cut ginger.The increasing PAL activity promoted the production of total phenols in fresh-cut ginger,which provided enough substrates for PPO and POD. The browning of fresh-cut ginger wasattributed to the oxidation of total phenols catalyzed by PPO and POD.
     (2) Eight compounds were identified from ginger extracts isolated by TLC. They were4-shogaol,1-dehydro-8-gingerdione,6-gingerol,8-shogaol,6-shogaol,8-gingerdione,1-dehydro-6-gingerdione and10-shogaol, which possess the functional group of3-methoxy-4-hydroxyphenyl.
     (3) The optimum temperature and pH of PPO was30℃and6.8, respectively. PPO wascompletely inactivated at100℃for2min. Vc, NaHSO3,L-Cys and citric acid couldsuppress the PPO activity, and the inhibitory potency was in the order of L-Cys> NaHSO3,Vc> citric acid. Ca~(2+)and Cu~(2+)could activate PPO activity while Al~(3+)and Mg~(2+)could inhibit the enzyme.
     (4) The optimum temperature and pH of POD was45℃and6.0, respectively. It couldbe inactivated at90℃for60s or at100℃for40s. Vc, EDTA-2Na, NaHSO3、citric acid,L-Cys could suppress the POD activity, and the inhibitory potency was in the order of Vc,NaHSO3,L-Cys> EDTA-2Na, citric acid. Ca~(2+), Mg~(2+), Fe~(3+)and Zn~(2+)could activate PODactivity at5mmol/L while Na+and Mn~(2+)could inhibit the enzyme.
     (5) The optimum temperature and pH value of PAL was60℃and8.2, respectively. Itcould be inactivated at90℃for15min or at100℃for10min, suggesting its high heatresistance. PAL could be activated by Mn~(2+), Zn~(2+), Cu~(2+)and Al~(3+), and suppressed by Ni~(2+).
     (6) Chitosan coating significantly suppressed browning and mass loss of fresh-cut ginger.Meanwhile, the decrease of total soluble sugar and vitamin C, and the increase of crude fiberwere inhibited. The optimal preservation quality of fresh-cut ginger was observed whenchitosan concentration was1.5%, which could delay the occurrence of the plasmolysis, inhibitthe degradation of cell wall as well as the disintegration of nucleus, plastid and mitochondria,maintain the integrity of cell structure, and consequently effectively prolong the shelf-life offresh-cut ginger.
     (7) The optimum conditions for color protection of fresh-cut ginger were Vc (0.3%),citric acid (0.5%) and L-Cys (0.4%), and the optimal coating preservation of fresh-cut gingerwas1.5%chitosan. Moreover, coating preservation exhibited a better preservation quality offresh-cut ginger.
引文
班兆军,李喜宏,李莉.灵武长枣多酚氧化酶的酶学特性研究[J].食品工业,2008,(3):4-6.
    蔡金星,李秀锦,刘秀凤.不同品种梨多酚氧化酶特性及其抑制剂的研究[J].河北农业技术师范学院学报,1999,13(1):55-58.
    陈功,余文华,徐德琼,李洁芝.净菜加工技术[M].北京:中国轻工业出版社,2005.
    陈海光,冯卫华,白卫东,林虹.鲜切竹笋褐变控制的研究[J].中国食品学报,2010,10(4):233-239.
    陈林林.柑橘皮精油对鲜切马铃薯褐变及冷却猪肉保鲜的影响[J].食品工业科技,2012,33(2):106-109.
    陈学红,秦卫东,高卉.莴苣中多酚氧化酶特性的研究[J].食品科学,2003,24(3):29-32.
    陈燕.生姜油树脂的提取、分析及其功能特性的研究[D].北京:中国农业大学食品学院,2001.
    陈燕,田泽,葛毅强,等.姜油树脂调节血脂和抗血小板聚集作用的研究[J].营养学报,2001,23(4):338-341.
    陈燕,周希贵,李淑燕,蔡同一.生姜油树脂的抗菌性研究[J].食品与发酵工业,2001,27(4):30-34.
    陈艳乐,申秀英,丁炳扬,等.薯蓣多酚氧化酶特性及褐变控制[J].食品科学,2003,24(10):59-62.
    程建军,马莺,杨咏丽等.苹果梨中多酚氧化酶酶学特性的研究[J].园艺学报,2002,29(3):261~262.
    崔庆新,董岩.生姜挥发油化学成分的GC-MS分析研究[J].聊城大学学报(自然科学版),2006,19(2):43-45.
    戴卿,侯温甫,王宏勋,等.葛根中多酚氧化酶性质及葛根护色的研究[J].食品科学,2009,30(20):430-432.
    丁捷,刘书香,张雪军,秦文,李正国.鲜切马铃薯复合褐变抑制剂组合的筛选[J].食品科学,2011,32(6):288-292.
    窦同心,孟祥春,张爱玉,张昭其.不同菠萝品种杀菌剂和褐变抑制剂对鲜切菠萝贮藏品质的影响[J].农产品加工·学刊,2011,(4):12-19.
    杜传来,郁志芳,董炳发.鲜切慈菇酶促褐变底物的分析确定[J].食品与发酵工业,2006,32(2):46-49.
    杜传来,郁志芳,王佳红,等.壳聚糖涂膜包装对鲜切莴苣保鲜效果的研究[J].包装与食品机械,2005,23(2):11-14.
    段艳欣,郭文武.多酚含量及多酚氧化酶活性与柑橘胚性愈伤组织褐化的关系[J].中国农学通报,2009,25(15):117-120.
    段玉权,冯双庆,马秋娟等.中华寿桃果肉多酚氧化酶的纯化研究[J].中国农业科学,2004,37(4):588-591.
    范文广,王庆国,毛春芳.热处理控制鲜切山药褐变研究[J].食品与发酵科技,2009,45(2):38-41.
    付聿成,王妮娅,杜金华.金帅苹果多酚氧化酶提取及部分酶学特性研究[J].食品工业科技,2006,27(2):59-62.
    高德民,张颖颖,樊守金.生姜品种根茎营养成分的研究[J].中国野生植物资源,2007,26(5):62-63.
    高晗,孙俊良,高愿军,南海娟,刘倩.气调包装对鲜切莲藕保鲜效果研究[J].食品科学,2008,29(10):612-614.
    高路.紫薯多酚氧化酶底物特异性的研究[J].食品科技,2009,34(4):34-37.
    高梦祥,杨凤琴.冬瓜多酚氧化酶的特性及抑制剂研究[J].食品科技,2008,(2):11-4.
    高梦祥,杨雪莲.鸭梨多酚氧化酶的特性及抑制剂研究[J].食品研究与开发,2007,28(9):50-53.
    高愿军,郝亚勤,南海娟,等.莲藕多酚氧化酶酶学性质的研究[J].食品研究与开发,2006,27(7):17-19.
    高愿军,吴炜炜,孟楠,樊振江.护色剂对鲜切莴笋褐变的影响[J].食品与机械,2010,26(4):46-48.
    耿艳艳.姜辣素对不同剂量60Co-γ射线辐射损伤小鼠治疗作用的研究[D].雅安:四川农业大学,2011.
    龚新明,崔彦红,关军锋,等.黄冠梨果皮多酚氧化酶的酶学特性[J].安徽农业科学,2008,36(31):13541-13543.
    郭英华,张振贤,关秋竹.姜的研究进展[J].长江蔬菜,2005,(9):38-42.
    哈楠.生姜提取物对造血系统辐射损伤的保护作用研究[D].吉林:吉林大学,2006.
    韩春雷.生姜总姜酚和总黄酮的制备及对PC12细胞拟脑缺血模型的保护作用研究[D].郑州:河南大学,2010.
    韩菊,魏福祥,王改珍,郝莉莉,李敏,王丽明.生姜中姜酚的性能研究[J].食品科技,2004,(4):63-65.
    韩涛,李丽萍.果实和蔬菜中的过氧化物酶[J].食品与发酵工业,1999,26(1):69-73.
    郝建军,康宗利.植物生理学[M].北京:化学工业出版社,2005.
    何文珊,严玉霞.生姜的化学成分及生物活性研究概况[J].中药材,2001,24(5):376~379.
    侯召华,王成荣,李文香,等.不同杂交后代苹果与富士苹果的多酚氧化酶特性比较[J].食品与机械,2007,23(1):58-61.
    胡明,胡云峰,陈嘉,陈杨,李仲群.鲜切梨褐变抑制的研究[J].食品工业科技,2008,l29(12):91-173.
    胡婉峰,赵洪雷,万琼红,等.莲藕多酚氧化酶的纯化方法研究[J].食品科学,2009,30(1):173-176.
    胡位荣,刘顺枝,黄惠娟,等.抗坏血酸与壳聚糖处理对鲜切香芋贮藏效果的研究[J].食品科学,2010,31(24):436-439.
    黄绍华,胡晓波,王震宙.山药中多酚氧化酶的活性测定及其护色研究[J].食品发酵工业,2005,31(6):27-29.
    黄锁义,罗燕,张婧萱,王三艳,陆辉宇.生姜总黄酮的提取及鉴别[J].时珍国医国药,2006,17(5):779-780.
    黄洲.不同剂量姜辣素对辐射损伤小鼠的抗氧化保护作用的研究[D].雅安:四川农业大学,2010.
    霍瑞贞,刘光东,栗印环.栗子多酚氧化酶催化动力学及抑制机理的研究[J].华南理工大学学报(自然科学版),1999,27(9):75-80.
    贾慧敏,韩涛,李丽萍,张海英.可食性涂膜对鲜切桃褐变的影响[J].农业工程学报,2009,25(3):282-286.
    姜爱丽,胡文忠,代喆,田密霞,刘程惠.箱式气调贮藏对鲜切富士苹果抗氧化系统的影响[J].食品与发酵工业,2011,37(10):187-191.
    姜爱丽,钟璐,胡文忠,赵丹,关棣锴,李沐华.3种褐变抑制剂对减轻鲜切苹果褐变效果的影响[J].食品与发酵工业,2010,36(7):45-48.
    蒋苏贞,宓穗卿,王宁生.姜辣素的化学成分研究概述[J].中药新药与临床药理,2006,17(5):386-389.
    蒋苏贞,谢芳斐.姜油对胃溃疡模型大鼠的影响[J].医药导报,2011,30(6):713-715.
    蒋苏贞,周婷婷.姜油对小鼠中枢神经系统的影响[J].中国实验方剂学杂志,2010,16(7):180-182.
    蒋益虹.百合褐变与多酚氧化酶和过氧化物酶活性关系的研究[J].浙江大学学报(农业与生命科学版),2003,9(5):518-522.
    匡芮,匡轩,朱海涛.生姜的功能因子与医疗保健作用[J].中国食物与营养,2007(2):52-53.
    李超,郑义,王乃馨,等.牛蒡多酚氧化酶的特性及酶促褐变的抑制[J].食品工业科技,2011,32(6):121-124.
    李桂琴,刘坤,张玉星.雪梨果肉多酚氧化酶的活性研究与纯化[J].食品科学,2007,28(12):297-300.
    李君兰,李怡华,赵秋玲等.鸡腿蘑多酚氧化酶特性研究[J].食品科学,2007,28(1):187-191.
    李乐.姜辣素抗氧化性研究[D].重庆:重庆大学,2004.
    李全宏,赵雅松,蔡同一,李淑燕.鲜切马铃薯褐变抑制效果研究[J].食品科学,2005,26(9):92-95.
    李伟锋,何玲,张江利,刘成明.生姜提取液对鲜切苹果贮藏品质的影响[J].北方园艺,2012,(3):157-160.
    李少燕.生姜挥发油的GC/MS分析[J].广西化工,1999,28(1):48-49.
    李秀锦.不同成熟期桃多酚氧化酶的研究[J].农业工程学报,1999,15(2):233-234.
    李延清,张艳芬,姜丽等.溧阳白芹PPO特性的研究[J].食品工业科技,2008,29(2):155-160.
    李银塔,刘扬瑞,迟玉森,邵仁东,步营,李可伟,任召珍,王哲恩,于玲.超临界CO2萃取姜挥发油及GC-MS分析[J].食品研究与开发,2009,30(3):121-125.
    连毅,乔旭光,李燕等.大蒜多酚氧化酶特性的研究[J].食品科学,2007,28(11):290-293.
    梁建荣,黄洁,苏茉,等.甘薯叶多酚氧化酶的分离纯化和部分性质研究[J].西南大学学报(自然科学版),2011,33(2):76-80.
    梁小玲.高氧气调包装对鲜切果蔬品质的影响[J].无锡:江南大学,2008.
    林茂.生姜有效成分的分离提取技术及组成研究[D].重庆:西南大学,2007.
    刘道宏.果蔬采后生理[M].北京:中国农业出版社,1997:67-70.
    刘伟,刘成梅,李明,涂宗财,梁瑞红.大孔吸附树脂纯化姜辣素研究[J].食品科学,2005,26(9):226-228.
    刘欣.食品酶学[M].北京:中国轻工业出版社,2011.
    刘雅嘉,李炜,衣杰荣.香菇多酚氧化酶酶学特性的研究[J].食品工业科技,2009,30(1):183-188.
    刘瑜,张卫明,单承莺,刘艳,姜洪芳.生姜挥发油抑菌活性研究[J].食品工业科技,2008,29(3):88-90.
    刘正实,刘成伦,殷钟意,杨海林,张映政,陈林.姜酚类化合物的稳定性及其抗氧化性能研究[J].重庆工商大学学报(自然科学版),2009,26(6):585-588.
    卢传坚,欧明,王宁生.姜的化学成分分析研究概述[J].中药新药与临床药理,2003,14(3):215-217.
    卢影,郑建仙.复合护色液对鲜切苹果的防褐变研究[J].现代食品科技,2009,25(9):1024-1028.
    陆胜民,徐亚光.鲜切竹笋冷藏过程中生理和生化变化的研究[J].中国食品学报,2004,4(2):25-30.
    罗金国,李洁,王清章.鲜切莲藕片的防褐变研究[J].食品研究与开发,2006,27(6):744-76.
    罗晓妙,史碧波,曾凡坤.黄花梨多酚氧化酶特性及防褐变处理[J].西昌学院学报(自然科学版),2006,20(4):44-47.
    罗兴武.鸭儿芹多酚氧化酶特性的研究[J].食品工业科技,2010,31(11):151-174.
    罗自生,张莉.壳聚糖/纳米SiOx复合物涂膜对鲜切竹笋品质和生理的影响[J].中国农业科学,2010,43(22):4694-4700.
    马文锦,刘树兴,凌建刚,等.茭白中多酚氧化酶活性的测定及护色效果研究[J].食品与发酵工业,2008,34(12):187-190.
    莫开菊,程超,黄鹏,吴淑华.生姜黄酮提取纯化及结构的初步鉴定[J].食品科学,2005,26(9):229-233.
    慕鸿雁,赵梅.复合护色液对鲜切牛蒡的防褐变研究[J].食品工业,2012,(2):60-62.
    南海娟,高愿军,郝亚勤.鲜切苹果抗褐变研究[J].食品与机械,2006,22(3):90-93.
    聂红,孟兰贞,张慧,钟玲,黄雪松.姜酚对中暑内毒素血症模型小鼠的影响[J].中国中西医结合杂志,2006,26(6):529-532.
    钮翠然.干姜中姜酚类成分的研究[D].吉林:吉林大学,2008.
    潘华柱.姜辣素对小鼠免疫系统辐射损伤的保护及作用机制研究[D].雅安:四川农业大学,2009.
    潘永贵,陈维信.鲜切莲藕组织中多酚氧化酶的分离纯化[J].食品与生物技术学报,2008,27(2):55-60.
    潘永贵,段琪,陈维信.壳聚糖涂膜处理对鲜切杨桃的保鲜效果[J].热带作物学报,2008,29(2):145-149.
    庞凌云,祝美云,李瑜,宋思聪.不同涂膜处理对鲜切铁棍山药品质的影响[J].食品与发酵工业,2011,37(6):225-228.
    彭芳.黄酮类化合物的生物学作用[J].大理医学院学报,1998,7(4):52-54.
    齐海萍,胡文忠,姜爱丽,田密霞.几种抗褐变剂对鲜切富士苹果褐变的抑制效果[J].江苏农业科学,2011,39(4):358-360.
    齐海萍,胡文忠,姜爱丽,田密霞.抗褐变剂对鲜切马铃薯保鲜技术的研究[J].安徽农业科学,2011,39(13):7690-7691.
    钱秋海.姜辣素在新型水貂呕吐模型的作用及机制[D].青岛:青岛大学,2010.
    秦卫东,钱寿凤,周瑞.仙人掌多酚氧化酶的特性及抑制研究[J].食品科学,2004,25(9):64-66.
    覃海元,杨昌鹏,陈智理,黄卫萍,潘嫣丽.草酸与柠檬酸抑制鲜切香蕉酶褐变的比较研究[J].食品工业科技,2011,32(4):75-77.
    邱松山,李喜宏,胡云峰,邢亚阁,张静.壳聚糖/纳米Tio:复合涂膜对鲜切辈莽保鲜作用研究[J].食品与发酵工业,2008,34(1):149-151.
    芮雯,冯毅凡,吴妍,薛元臻.干姜油中姜酚类成分的UPLC/Q-TOFMS分析[J].中草药,2008,39(5):667-668.
    沈金玉,黄家音,李晓莉.芦荟多酚氧化酶特性的研究[J].食品与发酵工业,2005,31(3):21-25.
    史辉,郑玉平,阮少江,等.枇杷多酚氧化酶酶学特性的研究[J].食品研究与开发,2010,31(7):9-13.
    宋莲军,唐贵芳,赵秋艳.富士苹果多酚氧化酶的提取及特性的研究[J].浙江农业科学,2009,(4):789-793.
    苏新国,蒋跃明,李月标,林文彬.4-HR对鲜切莲藕褐变以及贮藏品质的影响[J].食品科学,2003,24(12):142-145.
    孙亚青,李景明,李丽梅,倪元颖.姜精油的GC/MS分析研究[J].食品与发酵工业,2004,30(6):95-99.
    谭建宁.不同产地生姜挥发油化学成分的GC-MS研究[J].亚太传统医药,2011,7(4):23-25.
    汤凤霞,魏好程,曹禹.芒果多酚氧化酶的特性及抑制研究[J].食品科学,2006,27(12):156-160.
    陶育晖.生姜提取物对辐射损伤保护作用的研究[D].吉林:吉林大学,2004.
    田呈瑞,张京芳,陈锦屏,等.杏多酚氧化酶动力学研究[J].西北农业学报,1997,6(3):78-81.
    田密霞,胡文忠,朱蓓薇,姜爱丽,庞坤.抗坏血酸处理对鲜切水晶梨营养成分及褐变的影响[J].食品与发酵工业,2008,34(1):156-159.
    童刚平,邬应龙,陆胜民.鲜切荸荠褐变抑制剂组合的筛选[J].食品工业科技,2004,25(12):64-68.
    汪晓辉,卫莹芳,李隆云,龙飞,张盈娇.犍为干姜与生姜挥发油成分的比较研究[J].成都中医药大学学报,2006,29(3):54-55.
    王贵林,朱路.生姜油的抗炎作用[J].中药药理与临床,2006,22(5):26-28.
    王佳宏,郁志芳,杜传来.芋艿褐变底物及多酚氧化酶特性研究[J].食品工业科技,2008,29(2):141-145.
    王静,徐为民,诸永志,王道营,汪志君.贮藏温度对鲜切牛蒡褐变的影响[J].江苏农业学报,2008,24(4):492~496.
    王镜岩,朱圣庚,徐长法.生物化学(上册)[M].北京:高等教育出版社,2002.
    王军,刘惠霞,张薇,黄启福.生姜挥发油对脑缺血再灌注损伤的保护作用[J].中医杂志,2007,48(7):644-646.
    王丽,方磊,赵恒强,王珊珊,杜金华,王晓.高效液相色谱-电喷雾四级杆飞行时间质谱分析生姜中的姜辣素类化合物[J].中国中药杂志,2011,36(24):3467-3471.
    王亮,赵迎丽,李建华.鲜切果蔬加工品质的影响因素及解决方法[J].山西农业科学,2007,35(5):70-73.
    王曼玲,胡中立,周明全,宋运淳.植物多酚氧化酶的研究进展[J].植物学通报.2005,22(2):215-222.
    王娜,褚衍亮.大孔吸附树脂分离纯化生姜黄酮及抗氧化活性研究[J].中国调味品,2010,35(12):51-55.
    王啸.生姜活性部位与成分研究进展[J].中医研究,2009,22(12):53-55.
    王耀霞,杨志宏,岳旺,刘占涛,韩庆方.姜辣素在2种呕吐动物模型中止呕作用机制的探讨[J].沈阳药科大学学报,2009,26(2):134-137.
    王展华,李洁,高琼,王清章.洋葱提取液对鲜切马铃薯褐变的影响[J].食品研究与开发,2007,28(5):137-140.
    魏敏,周会玲,徐义杰,李可怡.贮藏温度对鲜切嘎啦苹果褐变的影响[J].北方园艺,2011,(17):160-163.
    吴继红,蔡同一.新疆无核白葡萄多酚氧化酶特性的研究[J].食品工业科技,2003,24(4):35-37.
    吴绪敏,周颖越,欧阳杰.高氧气调包装鲜切洋葱的研究[J].食品科学,2007,28(9):564-567.
    武彩霞.生姜有效部位对高脂血症大鼠血管内皮的保护作用[D].济南:山东大学,2005.
    夏斌,蔡飞,李彩蓉,吴基良.6-姜酚对大鼠局灶性脑缺血的保护作用[J].咸宁学院学报(医学版),2006,20(4):279-281.
    肖厚荣,杨红,蔡敬民.苹果多酚氧化酶的纯化与表征[J].生物学杂志,2009,26(3):41-44.
    熊何健,卢玉兰.荔枝壳中多酚氧化酶活性研究[J].食品科学,2006,27(12):182-185.
    徐芹,乔勇进,方强.砀山酥梨多酚氧化酶酶学特性及抑制效应的研究[J].食品科学,2008,29(4):74-77.
    徐静华,陈雪梅,赵余庆,李欢.姜辣素对动物实验性胃溃疡的影响[J].沈阳药科大学学报,2011,28(3):221-225.
    许正虹,刘璇,高彦祥.紫甘薯多酚氧化酶性质研究[J].食品与发酵工业,2006,32(8):18-23.
    杨昌鹏,胡艳妮,陈智理,等.杨桃多酚氧化酶的部分纯化及其特性研究[J].食品与发酵工业,2010,36(1):34-38.
    杨光宇,牟德华,赵广西,等.赵州雪花梨多酚氧化酶酶学特性研究[J].食品与机械,2010,26(5):10-12.
    杨明,余德顺,田弋夫,杨军,莫彬彬.超临界CO2萃取与水蒸气蒸馏提取姜油的GC-MS分析[J].贵州化工,2011,36(3):34-37.
    杨霞,舒晓斌,吴广辉,毕韬韬,高愿军.贮藏温度对鲜切莴笋品质的影响[J].食品与机械,2011,27(5):156-189.
    杨修斌,卢影,郑建仙.复合护色保鲜液抑制鲜切梨褐变的效果[J].食品与发酵工业,2010,36(11):156-160.
    于洁,陈耀容,王世祥,郑晓晖.生姜黄酮的提取及性能研究[J].食品科学,2009,30(24):108-113.
    余华,周洪波,何龙海,等.茄子多酚氧化酶的酶学特性研究[J].食品科学,2003,24(12):44-46.
    余悦,赵兴梅,邱春燕,白筱璐,邓文龙.姜油的抗炎镇痛作用研究[J].中药药理与临床,2008,24(6):50-51.
    郁志芳,李宁,赵友兴,康若.鲜切莲藕贮藏中的酚类物质变化及控制褐变的抑制剂组合筛选[J].南京农业大学学报,2003,26(1):78-81.
    郁志芳,陆兆新,宋雯雯,等.鲜切芦蒿PPO特性的研究[J].食品科学,2006,27(8):36-41.
    郁志芳,彭贵霞,夏志华,等.鲜切山药酶促褐变机理的研究[J].食品科学,2003,24(5):44-49.
    郁志芳,赵友兴,李宁,夏志华,彭贵霞.鲜切莲藕酶促褐变底物的分析确定[J].食品科学,2002,23(4):41-44.
    战琨友.姜油的化学成分分析及姜辣素的分离纯化研究[D].泰安:山东农业大学,2009.
    占习娟,陈义伦,刘宾,等.涩柿贮藏过程中PPO及褐变控制[J].食品科学,2007,28(4):341-344.
    张芳,张永茂,康三江.鲜切苹果褐变抑制的研究[J].中国食物与营养,2011,17(7):58-60.
    张福平,李秋红.温度对黄皮果实PAL、POD和PPO活性的影响[J].食品与发酵工业,2008,34(11):69-71.
    张福平,林小琼.火龙果果皮多酚氧化酶特性的研究[J].食品科学,2009,30(7):57-59.
    张福平,张喜春.佛手瓜多酚氧化酶酶学特性研究[J].食品科学,2010,31(1):161-164.
    张桂芝,杜鹃,付雪燕.阿魏菇中多酚氧化酶特性及其抗褐变剂的研究[J].新疆农业大学学报,2004,27(1):73-76.
    张宏,阎吉昌.生姜挥发油的提取及其化学成分的气相色谱-质谱分析[J].分析化学,1996,24(3):348-352.
    张君君,黄雪梅,黄世能,赵霞.鲜切黄藤笋褐变与3个酶活性变化的灰色关联分析[J].广东农业科学,2010,(12):96-98.
    张立华,孙晓飞,张艳侠,等.石榴多酚氧化酶的某些特性及其抑制剂的研究[J].食品科学,2007,28(5):216-219.
    张盛林,郑莲姬,钟耕.花魔芋中多酚氧化酶活力及抑制研究[J].中国食品学报,2007,7(4):51-55.
    赵徳婉,徐坤,艾希珍,等.生姜高产栽培[M].北京:金盾出版社,2005.
    赵德雪.生姜的抗呕吐作用研究[D].青岛:青岛大学,2007.
    赵东海,张建平,侯菊花.蘑菇中多酚氧化酶的酶学特性研究[J].食品与机械,2004,20(5):12-17.
    赵晋.生姜姜辣素的提取及其功能性质研究[D].重庆:西南大学,2008.
    赵喜亭,王会珍,李明军,张继澍.无硫护色剂对鲜切铁棍山药片酶促褐变的影响及其PPO特性研究[J].食品工业科技,2008,29(2):125-128.
    赵喜亭,王会珍,李明军,等.无硫护色剂对鲜切铁棍山药片酶促褐变的影响及其PPO特性研究[J].食品工业科技,2008,29(2):125-128.
    赵霞,郑谊,黄雪梅,等.低温贮藏对黄藤笋3种酶活性的影响[J].食品与发酵工业,2009,35(9):198-200.
    赵玉宏.高山花椰菜多酚氧化酶特性及酶褐变预防对策[J].湖北农业科学,2005,(4):74-76.
    郑杰,冯作山,刘宗林,等.新疆阿魏菇多酚氧化酶特性研究[J].食品科学,2005,26(3):56-59.
    郑莲姬,钟耕,张盛林.白魔芋中多酚氧化酶活性测定及其护色研究[J].西南大学学报(自然科学版),2007,29(2):118-121.
    周春梅,王欣,王俊城,等.白玉菇多酚氧化酶的酶学特性[J].食品与发酵工业,2010,36(5):5-8.
    周坚,万楚筠,沈汪洋.甜柿多酚氧化酶特性的研究及褐变控制[J].食品科学,2005,26(1):60-63.
    周山涛.果蔬贮运学[M].北京:化学工业出版社,1999:30-32.
    周向军,高义霞,袁毅君等.黑美人马铃薯多酚氧化酶的特性研究[J].食品工业科技,2011,32(1):115-123.
    周羽,谢振飞,耿艳艳,曾宪垠.姜辣素对60Co γ辐照损伤小鼠抗氧化功能和造血系统的防护作用[J].核技术,2011,34(9):658-662.
    朱路英,吴伟伟,孙杰,等.库尔勒梨多酚氧化酶的酶学特性[J].食品科学,2010,31(21):275-278.
    朱茂田,马力.生姜黄酮的分离和纯化工艺研究[J].食品研究与开发,2006,27(3):57-58.
    朱茂田,马力.姜辣素的分离和纯化工艺研究[J].食品工业科技,2006,27(6):130-131.
    诸永志,王静,王道营,沙琴,徐为民,汪志君[J].4-己基间苯二酚对鲜切牛蒡褐变及贮藏品质的影响[J].西南农业学报,2009,22(4):1061-1064.
    祝美云,李梅,朱世明.壳聚糖复合保鲜剂对鲜切李子品质的影响及其配方筛选[J].浙江农业学报,2009,21(4):375-378.
    祝义伟,周令国,肖琳,冯璨.姜辣素及其测定方法研究[J].农产品加工·学刊,2011,(3):77-80.
    宗亦臣.冬枣果实中酚类物质及其多酚氧化酶性质的研究[J].中国农学通报,2004,20(4):97-134
    Lamikanra O.,胡文忠.鲜切果蔬科学、技术与市场[M].北京:化学工业出版社,2008.
    Arzu zel, Ahmet Colak, Oktay Arslan, Melike Yildirim. Purification and characterisation ofa polyphenol oxidase from Boletus erythropus and investigation of its catalyticefficiency in selected organic solvents[J].Food Chemistry,2010,119:1044-1049.
    Barbaros Dincer, Ahmet Colak, Nese Aydin, Asim Kadioglu, Saadettin Guner.Characterization of polyphenoloxidase from medlar fruits (Mespilus germanica L.,Rosaceae)[J]. Food Chemistry,2002,77:1-7.
    Benjar Chutintrasri, Athapol Noomhorm. Thermal inactivation of polyphenoloxidase inpineapple puree[J]. LWT.,2006,39:492-495
    C A.M.C.N. Rocha, A.M.M.B. Morais.haracterization of polyphenoloxidase (PPO) extractedfrom “Jonagored” apple[J]. Food Control,2001,12:85-90
    Cheikh Ndiaye, Shi-Ying Xu, Zhang Wang. Steam blanching effect on polyphenoloxidase,peroxidase and colour of mango (Mangifera indica L.) slices[J].Food Chemistry.2009,113:92-95
    Chien Po-Jung, Sheu Fuu, Yang Feng-Hsu. Effects of edible chitosan coating on quality andshelf life of sliced mango fruit[J]. Journal of Food Engineering,2007,78(1):225-229.
    Chunyan Yu, Jian Sun, Xu Xiang, Bao Yang, Yueming Jiang.Variations in contents of()-epicatechin and activities of phenylalanine ammonialyase and polyphenol oxidase oflongan fruit during development[J]. Scientia Horticulturae,2010,125:230-232.
    D. Campaniello, A. Bevilacqua, M. Sinigaglia, M.R. Corbo. Chitosan: Antimicrobial activityand potential applications for preserving minimally processed strawberries[J]. FoodMicrobiology,2008,25:992-1000.
    Deepa, S. S.,&Arumughan, C.. Purification and characterization of soluble peroxidasefrom oil palm (Elaeis guineensis Jacq.) leaf[J]. Phytochemistry,2002,61:503-511.
    Demet Kavrayan, Tulin Aydemir. Partial purification and characterization ofpolyphenoloxidase from peppermint (Mentha piperita)[J]. Food Chemistry,2001,74:147-154.
    Dong Huaqiang, Cheng Liangying, Tan Jiahou, et al. Effect of chitosan coating on quality andshelf life of peeled litchi fruit[J]. Journal of Food Engineering,2004,64(3):355–358.
    F.A. Ayaz, O. Demir, H. Torun, Y. Kolcuoglu, A. Colak.Characterization ofpolyphenoloxidase (PPO) and total phenolic contents in medlar (Mespilus germanica L.)fruit during ripening and over ripening[J]. Food Chemistry,2008,106:291-298.
    G.I. Olivas, D.S. Mattinson, G.V. Barbosa-Canovas. Alginate coatings for preservation ofminimally processed ‘Gala’ apples[J]. Postharvest Biology and Technology,2007,45:89-96.
    G. Oms-Oliu, R. Soliva-Fortuny, O. Martin-Belloso. Edible coatings with antibrowning agentsto maintain sensory quality and antioxidant properties of fresh-cut pears[J]. PostharvestBiology and Technology,2008,50:87-94.
    Gabriela Rapeanu, Ann Van Loey, Chantal Smout, Marc Hendrickx. Biochemicalcharacterization and process stability of polyphenoloxidase extracted from Victoriagrape (Vitis vinifera ssp. Sativa)[J]. Food Chemistry,2006,94:253-261.
    Gisela Palma-Orozco, Alicia Ortiz-Moreno, Lidia Dorantes-álvarez, José G. Sampedro, HugoNájera.Purification and partial biochemical characterization of polyphenol oxidase frommamey (Pouteria sapota)[J]. Phytochemistry,2011,72:82-88.
    H. Hulya Orak. Total antioxidant activities, phenolics, anthocyanins, polyphenoloxidaseactivities of selected red grape cultivars and their correlations[J].ScientiaHorticulturae,2007,111:235-241.
    Huaqiang Dong, Liangying Cheng, Jiahou Tan, Kunwang Zheng, Yueming Jiang. Efects ofchitosan coating on quality and shelf life of peeled litchi fruit[J]. Journal of FoodEngineering2004,64:355-358.
    Jian Sun, Xu Xiang, Chunyan Yu, Jinyu Shi, Hongxiang Peng, Bao Yang,Shaoyu Yang, EnYang, Yueming Jiang. Variations in contents of browning substrates and activities ofsome related enzymes during litchi fruit development[J]. Scientia Horticulturae,2009,120:555-559.
    Jiang H,Solyom A M,Timmermann B N,et al.Characterization of gingerol: relatedcompounds in ginger rhizome(Zingiber officinale Rosc). by high: performance liquidchromatography/electrospray ionization mass spectrometry[J].Rapid Commun MassSpectrom,2005,19(20):2957-2964.
    Jian Sun, Yanli You, Elena García-García, Xing Long, Jubing Wang. Biochemical propertiesand potential endogenous substrates of polyphenoloxidase from chufa (Eleocharistuberosa) corms[J]. Food Chemistry,2010,118:799-803.
    Jhih-Ying Ciou, Hsin-Hung Lin, Po-Yuan Chiang, Chiun-C Wang, Albert Linton Charles. Therole of polyphenol oxidase and peroxidase in the browning of water caltrop pericarpduring heat treatment[J]. Food Chemistry.,2011,127:523-527.
    Jinsen Xu.The effect of low-temperature storage on the activity of polyphenol oxidase inCastanea henryi chestnuts[J]. Postharvest Biology and Technology,2005,38:91-98.
    Kwanele M. Mdluli. Partial purification and characterisation of polyphenol oxidase andperoxidase from marula fruit (Sclerocarya birrea subsp. Cafra)[J].Food Chemistry,2005,92:311-323.
    Krzysztof N. Waliszewski, Ofelia Márquez, Violeta T. Pardio. Quantification andcharacterisation of polyphenol oxidase from vanilla bean[J].Food Chemistry,2009,117:196-203.
    Li Guo, Ying Ma, John Shi, Sophia Xue. The purification and characterisation of polyphenoloxidase from green bean(Phaseolus vulgaris L.)[J]. Food Chemistry,2009,117:143-151.
    M.A. Rojas-Grau, M.S. Tapia, F.J. Rodriguez, A.J. Carmona, O.Martin-Belloso. Alginate andgellan-based edible coatings as carriers of antibrowning agents applied on fresh-cut Fujiapples[J]. Food Hydrocolloids,2007,21:118-127.
    M.B. Perez-Gago, M. Serra, M.A. del Rio. Color change of fresh-cut apples coated with wheyprotein concentrate-based edible coatings[J]. Postharvest Biology and Technology,2006,39:84-92.
    M.B. Perez-Gago, M. Serra, M. Alonso, M. Mateos, M.A. del Rio. Effect of whey protein-and hydroxypropyl methylcellulose-based edible composite coatings on color change offresh-cut apples[J]. Postharvest Biology and Technology,2005,36:77-85.
    M. Leja, A. Mareczek, J. Ben. Antioxidant properties of two apple cultivars during long-termStorage[J]. Food Chemistry,2003,80:303–307
    M. Umit Unal. Properties of polyphenol oxidase from Anamur banana (Musa cavendishii)[J].Food Chemistry,2007,100:909-913.
    M. Vargas, A. Albors, A. Chiralt, C. Gonzalez-Mart nez. Quality of cold-stored strawberries asaffected by chitosan–oleic acid edible coatings[J]. Postharvest Biology andTechnology,2006,41:164-171.
    Maria A. Rojas-Grau, Maria S. Tapia, Olga Martin-Belloso. Using polysaccharide-basededible coatings to maintain quality of fresh-cut Fuji apples[J]. LWT,2008,41:139-147.
    Mercedes Jimenez, Josefa Escribano-Cebrian, Francisco Garcia-Carmona. Oxidation of theflavonol fisetin by polyphenol oxidase[J].Biochimica etBiophysica Acta,1998,1425:534-542.
    Mustafa Erat, Halis Sakiroglu, O. Irfan Kufrevioglu. Purification and characterization ofpolyphenol oxidase from Ferula sp[J].Food Chemistry,2006,95:503-508.
    Oktay Arslan, Mahmut Erzengin, Selma Sinan, Ozen Ozensoy. Purification of mulberry(Morus alba L.) polyphenol oxidase by afnity chromatography and investigation of itskinetic and electrophoretic properties[J].Food Chemistry,2004,88:479-484.
    Ran Zhou,Yun Mo,Yunfei Li,Yanyun Zhao,Guixiang Zhang,Yunsheng Hu. Quality andinternal characteristics of Huanghua pears (Pyrus pyrifolia Nakai, cv. Huanghua) treatedwith different kinds of coatings during storage[J]. Postharvest Biology andTechnology,2008,49:171-179.
    Robson Maia Geraldine, Nilda de Fatima Ferreira Soares, Diego Alvarenga Botrel, Leticia deAlmeida Goncalves. Characterization and efect of edible coatings on minimallyprocessed garlic quality[J]. Carbohydrate Polymers,2008,72:403-409.
    Rosa M. Raybaudi-Massilia, Jonathan Mosqueda-Melgar, Olga Martín-Belloso. Ediblealginate-based coating as carrier of antimicrobials to improve shelf-life and safety offresh-cut melon[J]. International Journal of Food Microbiology,2008,121:313-327.
    Shivanand D. Jolad, R. Clark Lantz, Aniko M. Solyom, Guan Jie Chen,Robert B. Bates,Barbara N. Timmermann. Fresh organically grown ginger (Zingiber ofcinale):composition and efects on LPS-induced PGE2production[J]. Phytochemistry,2004,65:1937-1954.
    Saltveit, M. E. Wound induced changes in phenolic metabolism and tissue browning arealtered by heat shock[J].Postharvest Biol. Technol.2000,21:61-69.
    Serap Dogan, P nar Turan, Mehmet Dogan. Some kinetic properties of polyphenol oxidasefrom Thymbra spicata L. var. spicata[J]. Process Biochemistry,2006,41:2379–2385.
    Urszula Gawlik-Dziki, Urszula Szymanowska, Barbara Baraniak. Characterization ofpolyphenol oxidase from broccoli (Brassica oleracea var. botrytis italica) florets[J].Food Chemistry,2007,105:1047-1053.
    Urszula Gawlik-Dziki, Urszula Zlotek, Michal Swieca. Characterization of polyphenoloxidase from butter lettuce (Lactuca sativa var. capitata L.)[J]. Food Chemistry,2008:107:129-135.
    V.B. Anil Kumar, T.C. Kishor Mohan, K. Murugan. Purification and kinetic characterizationof polyphenol oxidase from Barbados cherry (Malpighia glabra L.)[J].FoodChemistry,2008,110:328-333.
    Vicente,Manuel,Gomez-Lopez. Some biochemical properties of polyphenol oxidase from twovarieties of avocado[J]. Food Chemistry,2002,77:163-169.
    Yue-Ming Jiang. Purification and some properties of polyphenol oxidase of longan fruit[J].Food Chemistry,1999,66:75-79.
    Zhao-Jian Gao, Xiao-Hong Han, Xing-Guo Xiao. Purification and characterisation ofpolyphenol oxidase from red Swiss chard (Beta vulgaris subspecies cicla)leaves[J].Food Chemistry,2009,117:342-348.
    Zhongxiang Fang, Min Zhang, Yunfei Sun, Jingcai Sun. Polyphenol oxidase from bayberry(Myrica rubra Sieb. et Zucc.)and its role in anthocyanin degradation[J]. Food Chemistry,2007,103:268-273.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700