重组人生长激素对梗阻性黄疸大鼠肠黏膜sIgA的作用
摘要
目的:探讨重组人生长激素对梗阻性黄疸大鼠肠黏膜sIgA的作用。
方法:将60只雄性wistar大鼠随机分为假手术(sham operation,SO)组、梗阻性黄疸(obstructive jaundice,OJ)组、重组人生长激素(recombinant humangrowth hormone,rhGH)组,每组各20只。将每组按7天、14天随机分为2个小组,每组10只。各组均无菌操作,沿肝十二指肠韧带寻找胆总管,SO组仅游离胆总管不予结扎,OJ组和rhGH组均双重结扎并横断胆总管。rhGH组于术后当日始,在双后肢内侧轮流皮下注射重组人生长激素(0.75iu/kg/d)。分别于术后第7天、14天采集门静脉血、回肠末端小肠组织。门静脉血液检测TB、DB和ALT值,采用偶氮基质显色法检测血浆内毒素。小肠组织HE染色后观察黏膜病理形态学改变,免疫组织化学(SP法)检测、Image Pro Plus(IPP)软件图像分析手段判定黏膜表面sIgA含量。
结果:(1) OJ组和rhGH组大鼠血TB、DB和ALT值均高于SO组(P＜0.05);OJ组和rhGH组大鼠血TB、DB和ALT值随着梗阻时间的延长而逐渐增高(P＞0.05);rhGH组与OJ组相比大鼠血TB、DB和ALT值有所降低,但无差异性(P＞0.05)。
(2) OJ组和rhGH组与SO组相比肠黏膜损伤严重(P＜0.01);OJ组随梗阻时间的延长肠黏膜损伤程度逐渐加重(P＜0.05);rhGH-7天组较OJ-7天组肠黏膜损伤程度轻(P＜0.05);rhGH-14天组较OJ-14天组肠黏膜损伤程度轻(P＜0.01)。rhGH-14天组较rhGH-7天组肠黏膜损伤程度减轻,但无差异性(P＞0.05)。
(3) OJ组和rhGH组血浆内毒素水平高于SO组(P＜0.05),并随着梗阻时间的延长而逐渐增高(P＜0.05);rhGH组与OJ组比较血浆内毒素水平明显降低(P＜0.05)。
(4) OJ组和rhGH组肠黏膜sIgA的表达水平低于SO组(P＜0.01);rhGH组较OJ组肠黏膜sIgA的表达水平高(P＜0.01);rhGH-14天较rhGH-7天组肠黏膜sIgA的表达水平有所增高,但无差异性(P＞0.05);OJ-14天组较OJ-7天组肠黏膜slgA的表达水平低(P＜0.05)。
结论:外源性的重组人生长激素能够改善梗阻性黄疸大鼠肠黏膜sIgA的表达,并能够保持肠黏膜结构的完整从而维护肠黏膜的屏障功能,降低血浆内毒素水平。
Aim:The study was undertaken to investigate the effect of recombinant growth hormone on sIgA of intestinal mucosa in rats with obstructive jaundice.
Methods:Sixty adult male Wastar Rats were randomly divided into 3 groups:Ⅰ, Sham Operation(SO,n=20);Ⅱ,Obstructive Jaundice(OJ,n=20);Ⅲ,Recombinant Human Growth Hormone(rhGH,n=20).In each group,rats were randomly assigned into 7days(n=10) and 14days(n=10) group.GroupⅠ:20 rats underwent laparotomy, but common bile duct(CBD) was not ligated and transected.GroupⅡandⅢ:40 rats underwent laparotomy,and CBD was ligated and transected.In the group of rhGH, Rats were undertaken subcutaneous injected of rhGH on posterior limb wall each day with dosage 0.75iu/kg/day.On postoperative 7days and 14days,the blood of portal vein and the terminal segments of ileum were harvested.To measure the TB、DB、ALT and endotoxin with portal blood.Ileum stained with hematoxylin and eosin was examined histologically and morphometrically.To examin sIgA of ileum with immunohistochemistry and identify it with Image Pro Plus(IPP).
Result:(1) TB、DB、ALT in the groups of OJ and rhGH were average higher than those in the groups of SO(P＜0.05);TB、DB、ALT in the groups of OJ and rhGH were gradually increased while the time going(P＞0.05);There were not significant difference between the groups of OJ and rhGH on TB、DB、ALT,(P＞0.05).(2) The ileal mucosa of group OJ and rhGH were acutely injuried than group SO (P＜0.01).While the time going the degree of ileal mucosa injuried in group OJ was increased(P＜0.05).The degree of ileal mucosa injuried in group rhGH-7days was lower than in group OJ-7days(P＜0.05);The degree of ileal mucosa injuried in group rhGH-14days was lower than in group OJ-14days(P＜0.01).The degree of ileal mucosa injuried in group rhGH-14days was lower than in group rhGH-7days,but there were not significant difference(P＞0.05).(3) The level of endotoxin in the groups of OJ and rhGH were higher than those in the groups of SO(P＜0.05);There were gradually increased as the time going(P＜0.05);The level of endotoxin in the group of rhGH were less than those in the group of OJ(P＜0.05).(4) The level of sIgA in the groups of OJ and rhGH were less than those in the group of SO(P＜0.01);The level of sIgA in the groups of rhGH were higher than in the group of OJ(P＜0.01); The level of sIgA in the group of OJ-14days was less than those in the group of OJ-7days(P＜0.05);There were not significant difference between the groups of rhGH-14days and rhGH-7days on the level of sIgA(P＞0.05).
Conclusion:(1) Ectogenic growth hormone can improve the sIgA secretion of intestine mucosal in rats with obstructive jaundice.It also can keep intestine mucosal structural integrated and lower the level of endotoxin in portal blood.
方法:将60只雄性wistar大鼠随机分为假手术(sham operation,SO)组、梗阻性黄疸(obstructive jaundice,OJ)组、重组人生长激素(recombinant humangrowth hormone,rhGH)组,每组各20只。将每组按7天、14天随机分为2个小组,每组10只。各组均无菌操作,沿肝十二指肠韧带寻找胆总管,SO组仅游离胆总管不予结扎,OJ组和rhGH组均双重结扎并横断胆总管。rhGH组于术后当日始,在双后肢内侧轮流皮下注射重组人生长激素(0.75iu/kg/d)。分别于术后第7天、14天采集门静脉血、回肠末端小肠组织。门静脉血液检测TB、DB和ALT值,采用偶氮基质显色法检测血浆内毒素。小肠组织HE染色后观察黏膜病理形态学改变,免疫组织化学(SP法)检测、Image Pro Plus(IPP)软件图像分析手段判定黏膜表面sIgA含量。
结果:(1) OJ组和rhGH组大鼠血TB、DB和ALT值均高于SO组(P＜0.05);OJ组和rhGH组大鼠血TB、DB和ALT值随着梗阻时间的延长而逐渐增高(P＞0.05);rhGH组与OJ组相比大鼠血TB、DB和ALT值有所降低,但无差异性(P＞0.05)。
(2) OJ组和rhGH组与SO组相比肠黏膜损伤严重(P＜0.01);OJ组随梗阻时间的延长肠黏膜损伤程度逐渐加重(P＜0.05);rhGH-7天组较OJ-7天组肠黏膜损伤程度轻(P＜0.05);rhGH-14天组较OJ-14天组肠黏膜损伤程度轻(P＜0.01)。rhGH-14天组较rhGH-7天组肠黏膜损伤程度减轻,但无差异性(P＞0.05)。
(3) OJ组和rhGH组血浆内毒素水平高于SO组(P＜0.05),并随着梗阻时间的延长而逐渐增高(P＜0.05);rhGH组与OJ组比较血浆内毒素水平明显降低(P＜0.05)。
(4) OJ组和rhGH组肠黏膜sIgA的表达水平低于SO组(P＜0.01);rhGH组较OJ组肠黏膜sIgA的表达水平高(P＜0.01);rhGH-14天较rhGH-7天组肠黏膜sIgA的表达水平有所增高,但无差异性(P＞0.05);OJ-14天组较OJ-7天组肠黏膜slgA的表达水平低(P＜0.05)。
结论:外源性的重组人生长激素能够改善梗阻性黄疸大鼠肠黏膜sIgA的表达,并能够保持肠黏膜结构的完整从而维护肠黏膜的屏障功能,降低血浆内毒素水平。
Aim:The study was undertaken to investigate the effect of recombinant growth hormone on sIgA of intestinal mucosa in rats with obstructive jaundice.
Methods:Sixty adult male Wastar Rats were randomly divided into 3 groups:Ⅰ, Sham Operation(SO,n=20);Ⅱ,Obstructive Jaundice(OJ,n=20);Ⅲ,Recombinant Human Growth Hormone(rhGH,n=20).In each group,rats were randomly assigned into 7days(n=10) and 14days(n=10) group.GroupⅠ:20 rats underwent laparotomy, but common bile duct(CBD) was not ligated and transected.GroupⅡandⅢ:40 rats underwent laparotomy,and CBD was ligated and transected.In the group of rhGH, Rats were undertaken subcutaneous injected of rhGH on posterior limb wall each day with dosage 0.75iu/kg/day.On postoperative 7days and 14days,the blood of portal vein and the terminal segments of ileum were harvested.To measure the TB、DB、ALT and endotoxin with portal blood.Ileum stained with hematoxylin and eosin was examined histologically and morphometrically.To examin sIgA of ileum with immunohistochemistry and identify it with Image Pro Plus(IPP).
Result:(1) TB、DB、ALT in the groups of OJ and rhGH were average higher than those in the groups of SO(P＜0.05);TB、DB、ALT in the groups of OJ and rhGH were gradually increased while the time going(P＞0.05);There were not significant difference between the groups of OJ and rhGH on TB、DB、ALT,(P＞0.05).(2) The ileal mucosa of group OJ and rhGH were acutely injuried than group SO (P＜0.01).While the time going the degree of ileal mucosa injuried in group OJ was increased(P＜0.05).The degree of ileal mucosa injuried in group rhGH-7days was lower than in group OJ-7days(P＜0.05);The degree of ileal mucosa injuried in group rhGH-14days was lower than in group OJ-14days(P＜0.01).The degree of ileal mucosa injuried in group rhGH-14days was lower than in group rhGH-7days,but there were not significant difference(P＞0.05).(3) The level of endotoxin in the groups of OJ and rhGH were higher than those in the groups of SO(P＜0.05);There were gradually increased as the time going(P＜0.05);The level of endotoxin in the group of rhGH were less than those in the group of OJ(P＜0.05).(4) The level of sIgA in the groups of OJ and rhGH were less than those in the group of SO(P＜0.01);The level of sIgA in the groups of rhGH were higher than in the group of OJ(P＜0.01); The level of sIgA in the group of OJ-14days was less than those in the group of OJ-7days(P＜0.05);There were not significant difference between the groups of rhGH-14days and rhGH-7days on the level of sIgA(P＞0.05).
Conclusion:(1) Ectogenic growth hormone can improve the sIgA secretion of intestine mucosal in rats with obstructive jaundice.It also can keep intestine mucosal structural integrated and lower the level of endotoxin in portal blood.
引文
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