园艺产品真空预冷过程温度检测与品质变化研究
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摘要
真空预冷依据水在低压下的快速蒸发吸收材料自身热量达到降温目的,是实现园艺产品采后冷链无缝对接、减少贮藏流通损失、延长其货架期的重要环节。
     温度是影响园艺产品品质的最主要因素之一。通常采用的检测园艺产品真空预冷过程温度的方法是把热电偶用胶带固定在材料上,该方法存在以下主要缺点:1)胶带阻碍水分的蒸发逸出,影响测温的准确性;2)对柔嫩组织,在不破坏表面结构的情况下准确测定其温度是很困难的;3)热电偶本身固有的局限性使每支热电偶每次只能检测与之接触表面上某一点的温度,而实际生产中又不可能布置无穷多的热电偶。另外,果蔬水分与其成分和微观结构之间存在复杂的束缚关系,真空预冷并不是理论上自由水的蒸发。真空压力下细胞内的水分状态和参与预冷程度如何,细胞膜系统和微观结构变化对水分汽化的作用如何,这些都是生产实际中遇到的根本问题。尤其对于细胞膜系统,需承受短时间的快速降压以及长时间的真空低压作用,膜系统是否被损伤或破坏尚无明确的研究结果。
     鲜切花是深受人们喜爱的一种园艺产品,具备较高的经济价值,较好的社会效益和生态效益。花瓣、叶子、茎杆等部位的物性差异影响其真空预冷过程的温度变化以及瓶插过程的主要品质。洋葱内表皮由尺寸较大的具有典型植物细胞结构的单层细胞组成,是研究细胞形态变化有代表性的理想材料。可靠的温度检测有利于准确判定预冷终点、控制冷却过程、提高预冷效果。对细胞膜系统稳定性的研究有助于掌握真空预冷园艺产品的品质变化,为科学、深入开发真空预冷技术提供理论依据。研究内容分以下四部分:
     一、利用热电偶检测鲜切花真空预冷过程温度变化,研究填充量和降压速率对月季切花预冷效果的影响。①发现不同数量花枝形成的局部环境对切花温度变化有明显影响(P<0.05)。10、50和120支处理切花不同部位温度变化和平均预冷终温不同,花束外围茎杆、叶子和花瓣的预冷终温均高于花束中心对应部位,花束中心区域的花朵花瓣预冷终温最低、降温速率最快。三种处理切花不同部位的温度变化显著区别于单支花,50支处理鲜重损失最小、瓶插寿命最长、预冷效果最好;②真空预冷可以不同程度地提高月季切花SOD、POD及CAT活性,有效地消除切花衰老过程中的活性氧,维持体内活性氧平衡以及细胞膜的稳定,延缓切花衰老进程;③不同降压速率(3.11×104 Pa min~(–1), 1.78×104 Pa min~(–1), 0.62×104 Pa min~(–1))对月季切花预冷品质有显著影响(P<0.05),且以中速降压(1.78×104 Pa min~(–1))处理对电导率、失重率、MDA和pH值的影响最小。
     二、采用红外热成像技术对鲜切花真空预冷不同部位的温度分布和变化进行实时检测。结果表明:①月季切花不同部位的平均降温速率不同(内层花瓣最慢、外层花瓣最快、而叶子和茎杆无明显差异),内层花瓣的预冷终点温度是外层花瓣的3.1倍。非洲菊切花舌状花瓣的平均降温速率最快而茎杆最慢,茎杆的预冷终点温度是舌状花瓣的9.9倍。不论月季还是非洲菊切花,不同部位的冷却速率大小顺序在不同的预冷阶段表现不一致,各个部位不同区域的温度分布亦不均匀;②在相同预冷条件下,分别用红外和热电偶检测月季和非洲菊切花温度,红外检测温度比热电偶检测温度分别低2.06–4.50°C和4.55~(–1)0.4°C。说明热电偶检测受固定方法、位置和水汽化的影响较大;③月季外层花瓣和非洲菊舌状花瓣的外缘为易发生冻伤部位。这就要求在鲜切花真空预冷过程中密切监测不同部位特别是花瓣外缘的温度变化,在保证预冷效果的同时避免冷伤发生;④月季和非洲菊单支花在瓶插期间的鲜重、气孔导度和蒸腾速率变化率均小于花束,平均预冷终点温度低于花束,瓶插寿命长于花束。延迟预冷对切花品质影响显著(P<0.05)。随延迟时间增加,预冷终点温度不断升高,瓶插寿命显著降低(P<0.05),花束主要品质受延迟时间的影响程度大于单支花。在本实验条件下,室温存放10 h,两种切花均失去市场价值。
     三、研究成膜型抗蒸腾剂对鲜切花真空预冷效果的影响。喷施抗蒸腾剂之后进行瓶插实验可以降低切花失水、延缓花朵开放进程、降低气孔导度、减少丙二醛积累、维持细胞膜完整性,切花寿命比直接进行瓶插实验延长32%。抗蒸腾剂和真空预冷的联合使用在快速去除田间热的同时有效地解决了水分损失过多的问题。其中,先喷施抗蒸腾剂再进行真空预冷处理的月季切花,其瓶插实验效果明显优于真空预冷后再进行抗蒸腾剂处理的切花(P<0.05),切花寿命比后者增加了12%。
     四、研究真空预冷对组织微观结构和物性的影响。①初步证实真空处理对洋葱表皮微观结构造成了较大的影响;②细胞膜系统在短时间内承受着快速降压和水分蒸发迁移微应力,液泡和细胞膜损伤导致胞内水分蒸发损失,从微观层面解释了组织水分过度损失的原因;③同时研究发现,膜损伤造成了花色素苷和电解质流失,导致细胞颜色减退以及电导率升高。质膜和细胞壁之间的间隙随真空室压强降低呈现先减小后增大的趋势;④扫描电镜观察显示细胞表面形态、细胞壁和胞间层发生了不同程度的形变,直接导致表皮组织抗张强度的降低;⑤改变降压速率对洋葱表皮细胞表面形态、质膜和细胞壁间距、水分损失、相对电导率、颜色和机械性能有明显影响(P<0.05)。在慢速和快速降压条件下,微观结构和物理性质发生了较大变化,而中速降压处理引起的破坏作用很小。
     本文采用宏观和微观研究相结合,实时图像分析与理化指标检测相结合的方法,分析比较红外热成像和传统热电偶温度检测结果及其对鲜切花后续贮藏特性的影响,解决鲜切花真空预冷过程温度难以准确检测和预冷终点难以判定的问题,并深入研究真空预冷压力变化对微观组织结构尤其是细胞膜系统的作用程度。
The principle of vacuum cooling is based on rapid evaporation of part of the moisture of the product under vacuum. The latent heat of product itself is moved and cooling is achieved. Vacuum cooling is a key link of the implementation of seamless joint of post harvest horticultural products, the loss reduction of storage and transportation and the shelf-life extension.
     One of the most important factors affecting the quality of horticultural crops is temperature. In previous papers, thermocouples were secured by tapes to measure the temperature. There are three major disadvantages to thermocouple temperature measurement:①causing a restriction in the escape of water vapor, which certainly affects the microclimate around the thermocouple.②It is very difficult to measure the petal temperature without damaging the thin fragile membrane with the thermocouple.③The inherent limitation of thermocouple is that each thermocouple can measure temperature only at the point on a surface that it is contacting. It is impractical to use more than a small number of thermocouples.
     Moreover, there has been little information about biological study, especially for cell membrane system and microstructure changes during vacuum cooling. There is a complex binding relationship between the moisture, constituents and microstructure of materials. The water evaporation during vacuum cooling is not the theoretical evaporation of free water. There are several practical productive problems such as how do the states and participation level in cooling of intracellular moisture, how does the effect on water vaporization affected by the changes of cell membrane system and microstructure. Especially to membrane system, which was subject to rapid pressure reduce in a short time and influenced by water evaporation and a long time vacuum environment. It was not clear whether the changes of pressure and forces could damage the cell membrane system.
     Cut flower is a very popular horticultural product, with high economic value, good social and ecological benefits. Different physical properties of petals, leaves and stems of flowers affect the temperature changes during vacuum cooling and the main quality during vase period. Onion epidermis composed by a single layer cells with large size and typical plant cell structure is an ideal material to study the changes in cell morphology. The reliable temperature measurement is beneficial to the judgment of pre-cooling end-temperature, control of cooling processes, and improvement of pre-cooling effects. The understanding and mastering of the mechanism of vacuum cooling is helpful for the scientific and deep development of vacuum cooling.
     The main research part can be divided into four parts as follows:
     1. Thermocouples were applied to measure the temperatures of cut flowers during vacuum cooling, and study the effects of loading and pressure reduction rates on the pre-cooling effects of cut roses.①The local environment made by different amounts of flowers had obviously influence (P<0.05) on the temperature especially for the inner parts of cut flowers. Though the temperature changes and average pre-cooling end-temperatures for different parts of 10, 50 and 120 flowers, the end-temperatures of external parts were higher than internal parts, the end-temperature of the internal petals were the lowest and the temperature reduction rates were the fastest. There were big differences between the individual flower and other three treatments. The fresh weight loss of 50 flowers treatment was the smallest and its vase life was the longest.②The activities of SOD, POD and CAT were improved. Consequently, the active oxygen produced during the aging process of cut flower was scavenged effectively, and the balance of active oxygen in vivo and the stability of cell membrane were maintained.③Different pressure reduction rates (3.11×104 Pa min~(–1), 1.78×104 Pa min~(–1), 0.62×104 Pa min~(–1)) had significant effects (P<0.05) on the pre-cooling quality of cut roses. The moderate pressure reduction rate had the smallest influence on relative electrical conductivity, weight loss, MDA and pH.
     2. Thermal infrared imaging was used to realize the real-time measurement of the distributions and changes of temperatures of cut flowers, and compared with the results made by thermocouples.①Thermal infrared imaging showed that the average cooling rates of different parts of the rose were different (the slowest was the inner petals, the fastest was the outer petals, and there were no remarkable differences between the leaf and the stem) , the final temperature of cooling of the inner petal was 3.1 times higher than that of the outer petal. For cut gerberas, the fastest was the ligule-shape petal, and the slowest was the stem, the final temperature of cooling of the stem was 9.9 times higher than that of the ligule-shape petal. The sequences of cooling rates of different parts of rose and gerbera flowers during different cooling stages were different, and the temperature distributions of different parts measured with thermal infrared camera were non-uniform.②Under the same pre-cooling conditions, the temperatures detected by the thermal infrared imaging were ac. 2.46 to 4.50°C and 4.55 to 10.4°C lower than the results measured by thermocouples of cut rose and gerbera flowers, respectively. The results showed that thermocouple temperature measurement was affected by fixation method, location and water vapor.③The parts which chilling injury occurred easily were the outside edges of outer petals of cut roses and ligule-shape petal of gerbera flowers. In order to obtain better pre-cooling effects without chilling injury, temperatures of the different parts of cut flowers especially for the outside edges of outer petals must be monitored closely during vacuum cooling.④The changes of relative fresh weight, stomatal conductivity and transpiration of the individual flowers were bigger than bunches of flowers for both cut roses and gerberas. The average pre-cooling end-temperatures of bunches of flowers were higher than the individual flowers. Not only direct but also delay pre-cooling of cut roses and gerberas, the vase life of bunches of flowers were shorter than the individual flowers. Furthermore, the vase life of individual and bunches of flowers were decreased obviously (P<0.05) with time during vase period. In this experiment, two kinds of flowers lost their ornamental values after ten hours storage at room temperature.
     3. Effects of antitranspirant and vase solution on the quality of cut flowers were studied. Spray application of antitranspirant decreased the fresh weight loss, delayed the process of flowering, slowed down the decrease rate of stomatal conductivity, decreased transpiration and the accumulation of MDA and maintained the integrity of cell membrane. The vase life was extended by 32% compared with the control flowers. The combined use of antitranspirant and vacuum cooling could effectively improve the pre-cooling quality. Furthermore, the experimental effects of spraying antitranspirant followed by vacuum cooling were better than the treatment of vacuum cooling followed by the application of antitranspirant (P<0.05), and the vase life was extended by 12% compared with the treatment of vacuum cooling followed by the application of antitranspirant.
     4. Effect of vacuum treatment on microstructure and physical properties of tissue.①The present study has shown that vacuum treatment induced the changes of onion epidermis microstructure.②For cellular membrane system, which was subject to rapid pressure reduce in a short time and influenced by microstress caused by water evaporation. Surface morphology and that cell rupture occurred result from the joint effects of water loss and pressure gradient. The vacuole and cell membranes were damaged which led to loss of intracellular water, which explained the reason for excessive loss of tissue water at micro-level. It presented preliminary proof of the excessive water loss induced by vacuum treatment.③Disruption and damage to cell membranes altered permeability led to the loss of electrolyte and anthocyanin, resulting in fade in cellular color and increase of electrical conductivity. The spaces between the membrane and cell wall decreased first and then increased with the decreasing of pressure in vacuum chamber.④SEM micrographs indicated that vacuum treatment induced significant changes on cell surface morphology, cell wall and intercellular layers of onion epidermis, which led to the decrease of tensile strengths of epidermal tissue.⑤Changed the pressure reduction rate had obvious effects on the surface morphology, spaces between the cell membrane and cell wall, mass, REC, color, and mechanical properties. Of the 3 pressure reduction rates, treatments at the slow and fast pressure reduction rates led to remarkable effects on the cell membrane integrity of the onion epidermis, while treatment at the medium pressure reduction rate caused less damage.
     This paper adopts the method of combination of macro-and microscopic, and the combination of real-time image analysis and detection of physiochemical indexes. The effects of temperature changes measured by thermal infrared imaging and thermocouples during vacuum cooling on the storing properties of cut flowers were analyzed and compared, so as to overcome some shortcomings such as the difficulties of temperature detection and pre-cooling end-temperature judgment of cut flowers during vacuum cooling. Furthermore, the impacts of vacuum cooling on the microstructure, especially for cell membrane system were studied.
引文
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