捻转补泻手法对应激性高血压大鼠Ach-NO-cGMP信号转导通路影响的机制研究
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摘要
目的
观察针刺捻转补泻手法对应激性高血压大鼠模型Ach-NO-cGMP信号转导通路中乙酰胆碱(Ach)、一氧化氮合成酶(NOS)、环鸟苷酸(cGMP)表达的影响,揭示捻转补泻手法与效应的相关性及捻转补泻手法产生不同生物学效应的机制,以期为针刺捻转补泻手法的实用性和科学性提供依据,促进针刺补泻手法在针灸临床中的应用和发展。
方法
清洁级健康雄性Wistar大鼠60只,体重220～280g。按照随机数字表分为5组,每组12只,A：正常组、B：模型对照组、C：针刺对照组、D：针刺补法组、E：针刺泻法组五组。空白组不做处理,其他各组均采用电击加噪声刺激法制成应激性高血压动物模型,并在造模的前1天,造模第3、6、9、12、15天的刺激后2小时测量大鼠收缩压。15天造模成功后,每天上午分别对针刺组、补法组、泻法组大鼠的双侧“太冲”穴施以针刺不做手法、捻转补法和捻转泻法操作。捻转手法幅度为360°/次,60次/min,持续捻针1min,留针20min。治疗7天后,对实验动物进行断头处死,取血和胸主动脉,用ELISA法检测血浆和胸主动脉组织中的Ach、NOS、cGMP含量。
结果
1应激性刺激和不同针刺手法对各组大鼠收缩压的影响
经过15天应激性造模后,各造模组大鼠收缩压显著上升,与空白组相比有显著差异(p<0.01),并且造模各组之间没有显著差异(p>0.05)；经过7天针刺处理之后,B组大鼠血压波动不明显,与A组比较具有显著差异(p<0.01)；C组、D组、E组血压均下降,E组血压下降最显著,D组和针刺组与E组比较差异显著(p<0.05)；D组、E组与B组比较,血压显著下降(p<0.01)；C组与B组比较,血压下降(p<0.05)。
2针刺捻转补泻对各组大鼠血浆中Ach的影响
B组大鼠血浆中Ach显著下降,与A组相比有显著差异(p<0.01)；C组、D组、E组大鼠血浆中Ach含量均上升,与B组比较具有显著差异(p<0.01)；E组大鼠血浆中Ach上升最明显,并与c组和D组比较均有显著差异(p<0.01),C组和D组大鼠血浆中Ach上升,二者比较差异不显著(p>0.05)。3针刺捻转补泻对各组大鼠主动脉组织中NOS的影响
在主动脉组织NOS中,B组主动脉组织NOS含量显著下降,与A组比较差异显著(p<0.01)；C组、D组、E组中NOS含量均上升,C组NOS含量上升与B组比较有差异(p<0.05)；D组和E组上升显著,并与B组比较差异显著(p<0.01)；其中D组上升最显著,D组与C组比较有差异(p<0.05)；E组和C组比较NOS含量无明显差异(p>0.05),D组和E组NOS含量上升没有明显差异(p>0.05)。4针刺捻转补泻对各组大鼠主动脉组织中cGMP的影响
在主动脉cGMP中,B组主动脉组织cGMP含量显著下降,与A组比较差异显著(p<0.01)；C组、D组、E组中cGMP含量与造模组比较均上升,以D组上升最明显,D组、E组与B组比较cGMP含量上升具有显著差异(p<0.01)；C组与E组比较cGMP含量上升具有差异(p<0.05)；C组与D组比较cGMP含量上升具有显著差异(p<0.01),D组和E组比较,cGMP含量上升不具有显著差异(p>0.05)。
结论
1捻转补泻手法有降低应激性高血压的作用,捻转补法和捻转泻法的降压效应存在差异；
2捻转补泻手法能升高应激性高血压大鼠模型中血浆Ach、主动脉组织NOS、主动脉组织cGMP的含量；
3捻转补泻手法调节血压的机制之一可能是通过Ach-NO-cGMP信号转导通路实现的。
Objective
The research aims at observing the effect on Ach, NOS, cGMP on Ach-NO-cGMP singal transduction pathway of stress-induced hypertension rats treated by twirling reinforcing and reducing manipulation, revealing the mechanism of relativity between twirling reinforcing and reducing manipulation and the different biology effect, providing an experimental evidence to twirling reinforcing and reducing manipulation, and promoting the popularization of manipulation in acupuncture practice.
Method
60 clean male Wistar rats with weight ranging from 220g to 280g were divided into A(blank group), B(model group), C(needling group), D(reinforcing group), and E(reducing group) five groups randomly. Electric shock and noise were applied to stimulate the rats into stress-induced hypertension models except group A. Each rat was treated by 1 minute manipulation and 20 minutes needle retaining. The treatment lasted for 7 days and once each day. After the treatment Ach, NOS, cGMP contents in plasma and aortic were detected by means of radio immuno assay and ELISA.
Result
1 Systolic blood pressure changes of stress-induced hypertension rats after electric shock and noise stimulation and acupuncture treatment
The systolic blood pressure of each stimulated group increased significantly after 15 days lectric shock and noise stimulation. Campared with group A the difference were significant (p<0.01). The differences of each stimulated group were not obvious (p>0.05). After 7 treatment days, the systolic blood pressure of group B were stable and different to the group A (p<0.01); the the systolic blood pressure of group C, group D and group E all decreased, the group E were the most obvious, and the group D and group C were sinificient different to group E (p<0.05); group D and group E campared with group B were obviously different (p<0.01); and the differences of systolic blood pressure were significant between group C and group B (p<0.05).
2 Changes of Ach in plasma of stress-induced hypertension rats
The Ach in plasma decreased significantly in group B, which were obviously different to group A (p<0.01); the Ach of group C,D and E all increased and different to group B (p<0.01); the Ach of group E inceased most obviously, which were different to group C and group D (p<0.01); however, there were no signifcient difference in group C and D (p>0.05). 3 Changes of NOS in aortic of stress-induced hypertension rats
The NOS in aortic decreased significantly in group B, which were obviously different to group A (p<0.01); the NOS of group C,D and E all increased, there were significient differences between group C and group B (p<0.05); campared group D and E with group B, the differences were obvious (p<0.05), in which the NOS in group D was the highest; there were different between group D and group C (p<0.05); however, there were no signifient differences between group E and C and group D and E (p>0.05).
3 Changes of cGMP in aortic of stress-induced hypertension rats
The cGMP in plasma decreased significantly in group B, which were obviously different to group A (p<0.01); the cGMP of group C,D and E all increased, in which the group D was the highest; campared group D and E with group B, the differences were obvious (p<0.01) campared group C and group E the differences were signifcient (p<0.05); campared group C and group D the differences were more signifcient (p<0.01); however, there were no signifient differences between group E and C and group D and E (p>0.05)
Conclusion
1 Twirling reinforcing and reducing manipulation could reduce stress-induced hypertension, the antihypertensive effect are difference between twirling reinforcing and reducing manipulation;
2 Twirling reinforcing and reducing manipulation could increase the contents of Ach, NOS, cGMP in plasma and aortic of stress-induced hypertension rats;
3 Ach-NO-cGMP singal transduction pathway could one of the blood pressure regulating mechanism of twirling reinforcing and reducing manipulation.
观察针刺捻转补泻手法对应激性高血压大鼠模型Ach-NO-cGMP信号转导通路中乙酰胆碱(Ach)、一氧化氮合成酶(NOS)、环鸟苷酸(cGMP)表达的影响,揭示捻转补泻手法与效应的相关性及捻转补泻手法产生不同生物学效应的机制,以期为针刺捻转补泻手法的实用性和科学性提供依据,促进针刺补泻手法在针灸临床中的应用和发展。
方法
清洁级健康雄性Wistar大鼠60只,体重220～280g。按照随机数字表分为5组,每组12只,A：正常组、B：模型对照组、C：针刺对照组、D：针刺补法组、E：针刺泻法组五组。空白组不做处理,其他各组均采用电击加噪声刺激法制成应激性高血压动物模型,并在造模的前1天,造模第3、6、9、12、15天的刺激后2小时测量大鼠收缩压。15天造模成功后,每天上午分别对针刺组、补法组、泻法组大鼠的双侧“太冲”穴施以针刺不做手法、捻转补法和捻转泻法操作。捻转手法幅度为360°/次,60次/min,持续捻针1min,留针20min。治疗7天后,对实验动物进行断头处死,取血和胸主动脉,用ELISA法检测血浆和胸主动脉组织中的Ach、NOS、cGMP含量。
结果
1应激性刺激和不同针刺手法对各组大鼠收缩压的影响
经过15天应激性造模后,各造模组大鼠收缩压显著上升,与空白组相比有显著差异(p<0.01),并且造模各组之间没有显著差异(p>0.05)；经过7天针刺处理之后,B组大鼠血压波动不明显,与A组比较具有显著差异(p<0.01)；C组、D组、E组血压均下降,E组血压下降最显著,D组和针刺组与E组比较差异显著(p<0.05)；D组、E组与B组比较,血压显著下降(p<0.01)；C组与B组比较,血压下降(p<0.05)。
2针刺捻转补泻对各组大鼠血浆中Ach的影响
B组大鼠血浆中Ach显著下降,与A组相比有显著差异(p<0.01)；C组、D组、E组大鼠血浆中Ach含量均上升,与B组比较具有显著差异(p<0.01)；E组大鼠血浆中Ach上升最明显,并与c组和D组比较均有显著差异(p<0.01),C组和D组大鼠血浆中Ach上升,二者比较差异不显著(p>0.05)。3针刺捻转补泻对各组大鼠主动脉组织中NOS的影响
在主动脉组织NOS中,B组主动脉组织NOS含量显著下降,与A组比较差异显著(p<0.01)；C组、D组、E组中NOS含量均上升,C组NOS含量上升与B组比较有差异(p<0.05)；D组和E组上升显著,并与B组比较差异显著(p<0.01)；其中D组上升最显著,D组与C组比较有差异(p<0.05)；E组和C组比较NOS含量无明显差异(p>0.05),D组和E组NOS含量上升没有明显差异(p>0.05)。4针刺捻转补泻对各组大鼠主动脉组织中cGMP的影响
在主动脉cGMP中,B组主动脉组织cGMP含量显著下降,与A组比较差异显著(p<0.01)；C组、D组、E组中cGMP含量与造模组比较均上升,以D组上升最明显,D组、E组与B组比较cGMP含量上升具有显著差异(p<0.01)；C组与E组比较cGMP含量上升具有差异(p<0.05)；C组与D组比较cGMP含量上升具有显著差异(p<0.01),D组和E组比较,cGMP含量上升不具有显著差异(p>0.05)。
结论
1捻转补泻手法有降低应激性高血压的作用,捻转补法和捻转泻法的降压效应存在差异；
2捻转补泻手法能升高应激性高血压大鼠模型中血浆Ach、主动脉组织NOS、主动脉组织cGMP的含量；
3捻转补泻手法调节血压的机制之一可能是通过Ach-NO-cGMP信号转导通路实现的。
Objective
The research aims at observing the effect on Ach, NOS, cGMP on Ach-NO-cGMP singal transduction pathway of stress-induced hypertension rats treated by twirling reinforcing and reducing manipulation, revealing the mechanism of relativity between twirling reinforcing and reducing manipulation and the different biology effect, providing an experimental evidence to twirling reinforcing and reducing manipulation, and promoting the popularization of manipulation in acupuncture practice.
Method
60 clean male Wistar rats with weight ranging from 220g to 280g were divided into A(blank group), B(model group), C(needling group), D(reinforcing group), and E(reducing group) five groups randomly. Electric shock and noise were applied to stimulate the rats into stress-induced hypertension models except group A. Each rat was treated by 1 minute manipulation and 20 minutes needle retaining. The treatment lasted for 7 days and once each day. After the treatment Ach, NOS, cGMP contents in plasma and aortic were detected by means of radio immuno assay and ELISA.
Result
1 Systolic blood pressure changes of stress-induced hypertension rats after electric shock and noise stimulation and acupuncture treatment
The systolic blood pressure of each stimulated group increased significantly after 15 days lectric shock and noise stimulation. Campared with group A the difference were significant (p<0.01). The differences of each stimulated group were not obvious (p>0.05). After 7 treatment days, the systolic blood pressure of group B were stable and different to the group A (p<0.01); the the systolic blood pressure of group C, group D and group E all decreased, the group E were the most obvious, and the group D and group C were sinificient different to group E (p<0.05); group D and group E campared with group B were obviously different (p<0.01); and the differences of systolic blood pressure were significant between group C and group B (p<0.05).
2 Changes of Ach in plasma of stress-induced hypertension rats
The Ach in plasma decreased significantly in group B, which were obviously different to group A (p<0.01); the Ach of group C,D and E all increased and different to group B (p<0.01); the Ach of group E inceased most obviously, which were different to group C and group D (p<0.01); however, there were no signifcient difference in group C and D (p>0.05). 3 Changes of NOS in aortic of stress-induced hypertension rats
The NOS in aortic decreased significantly in group B, which were obviously different to group A (p<0.01); the NOS of group C,D and E all increased, there were significient differences between group C and group B (p<0.05); campared group D and E with group B, the differences were obvious (p<0.05), in which the NOS in group D was the highest; there were different between group D and group C (p<0.05); however, there were no signifient differences between group E and C and group D and E (p>0.05).
3 Changes of cGMP in aortic of stress-induced hypertension rats
The cGMP in plasma decreased significantly in group B, which were obviously different to group A (p<0.01); the cGMP of group C,D and E all increased, in which the group D was the highest; campared group D and E with group B, the differences were obvious (p<0.01) campared group C and group E the differences were signifcient (p<0.05); campared group C and group D the differences were more signifcient (p<0.01); however, there were no signifient differences between group E and C and group D and E (p>0.05)
Conclusion
1 Twirling reinforcing and reducing manipulation could reduce stress-induced hypertension, the antihypertensive effect are difference between twirling reinforcing and reducing manipulation;
2 Twirling reinforcing and reducing manipulation could increase the contents of Ach, NOS, cGMP in plasma and aortic of stress-induced hypertension rats;
3 Ach-NO-cGMP singal transduction pathway could one of the blood pressure regulating mechanism of twirling reinforcing and reducing manipulation.
引文
[1]刘炜,王朝阳,刘清国,等.《内经》针刺补泻探讨.北京中医药大学学报,2010,33(2)：141-144.
[2]王苓,曾永蕾.“捻转”如何定“补泻”.针灸临床杂志,2001,17(11)：3-4.
[3]针灸甲乙经校释.山东中医学院校释.北京：人民卫生出版社,2009：546.
[4]许红君.从“北半球左漩涡”浅谈“捻转补泻法”.光明中医,2008,23(2)：166-167.
[5]孙星亮等.浅谈热补针法的起源及现状.陕西中医,2006,27(1)：90-91.
[6]卞金玲,张春红.石学敏院士针刺手法量学的概念及核心.中国针灸,2003,23(5)：287-289.
[7]郝应林.针刺“捻转补泻法”的探讨研究.世界中医药,2009,4(2)：102-104.
[8]文洪,王晓英.捻转补泻法与经脉循行方向的关系.针灸临床杂志,2005,21(5)：3-5.
[9]金百仁.捻转补泻探讨.浙江中医杂志,1991,26(2)：49-51.
[10]魏连海.重新认识捻转补泻针刺法.上海针灸杂志,2004,23(4)：13.
[11]睢明河,周宇姝,马文珠,等.大小刺激量捻转补泻法对血虚证大鼠红细胞计数的影响.针刺研究,2004,29(3)：213-216.
[12]董善京,孟丹.不同凉泻针刺手法对致热大鼠血浆内毒素水平影响的实验研究.河南中医药学刊,2001,16(6)：20-23.
[13]孙六合,董善京.凉泻手法对内毒素致热大鼠血清超氧化歧化酶水平的影响.时珍国医国药,2001,12(6)：546-549.
[14]方剑乔,林咸明,等.不同手法针刺对内毒素致热家兔体温的影响.针刺研究,1996,21(3)：50-52.
[15]王丽,王朝阳,支建梅,等.捻转补泻手法对应激性高血压大鼠颈交感神经放电的影响.北京中医药大学学报,2009,32(2)：142-145.
[16]王子梅,钱峰,陆纲,等.电刺激引发骨骼肌细胞钙震荡对nAChRy启动子活性的影响.中国生物化学与分子生物学报,2003,19(3)：283-285.
[17]关景芳,王理.针刺小鼠足三里穴组织化学实验观察.辽宁中医志,2002,29(6)：359-361.
[18]关卫,王芳.石氏捻转补泻手法对健康小鼠能量代谢相关酶的影响.天津中医,2002,19(5)：29-32.
[19]杨华元,钟小红,刘堂义,等.针刺仿真手法对高血压大鼠血压及心肌血管紧张素Ⅱ的影响.针刺研究,2008,33(3)：186-189.
[20]王君,张宝文,于新宇,等.疾徐捻转泻法针刺足三里对高血压家兔的降压作用.中国临床康复,2006,10(7)：116-117.
[21]邹移海,何智明,汤建华.捻转补泻手法对大鼠小肠系膜透明窗肥大细胞的影响.广东解剖学通报,1993,15(2)：107-111.
[22]郭永明.醋酸型胃溃疡大鼠PGE2变化及不同针刺手法的调节效应.辽宁中医杂志,2002,29(6)：313-315.
[23]王彩虹,王银平,许建敏,等.不同刺激量的捻转手法对健康人皮肤温度的影响.上海针灸杂志,2007,26(7)：33-35.
[24]王艳君,杨克涛.捻转补泻手法对健康人穴位皮肤温度的影响.中华实用中西医杂志,2003,3(16)：724-725.
[25]李平,关卫,等.捻转补泻手法针刺足三里穴对脘腹部皮肤温度的影响.天津中医学院学报,2002,19(4)：51-53.
[26]李平,关卫,等.捻转补泻手法针刺合谷穴对其局部皮肤温度的影响.天津中医学院学报,2002,21(3)：31-33.
[27]何智明.针刺补泻手法的实验研究.上海针灸杂志,1983,(2)：20-23.
[28]吕光,孙立明,李平.胆囊结石术后胃肠功能紊乱的针刺研究.陕西中医,2010,21(5)：587-589.
[29]许冠荪,郭原,张群群.针刺补泻手法对家兔实验性胃节律紊乱的影响.安徽中医学院学报,1992,11(2)：36-39.
[30]曹利民.浅谈针刺补泻手法.江苏中医,1993,14(8)：27-29.
[31]李万瑶.针刺提插补泻手法的胃电信息检测观察.中国针灸,1993,3(13)：29-32.
[32]李成宏,王玉中,郭新侠.针四关穴为主治疗原发性痛经临床观察.中国针灸,2008,28(3)：187-190.
[33]陶晓雁,孙彩霞,杨金亮.重刺激眼周腧穴治疗青少年近视临床观察.中国针灸,2008,28(3)：193-195.
[1]王舒,杨华,石学敏.国外原发性高血压的发病机制及诊治研究新进展.中西医结合心脑血管病杂志,2010,8(3)：342-344.
[2]张砀.血管活性肠肽的研究进展.医学综述,2009,15(21)：32-35.
[3]Schunkert H, Dzau V L Tang S S, et al. Increased rat cardiac angiotensin converting enzyme activity and m RNA expression in pressure overload left ventricular hypertrophy. Effects on Coronary resistance contractility, and relaxation. J Clin Invest,1990, 86(6):1913-1920.
[4]何昆仑,郑秋甫,牟善初,等.自发性高血压大鼠心肌肥大与心肌MAPK及AngⅡ的实验研究.解放军医学杂志,1998,23(2)：119-121.
[5]汪平,王宏.脑出血患者精氨酸加压素与水代谢相关性研究.中华神经外科疾病研究杂志,2009,8(4)：294-196.
[6]郝星,冼惠珍,马淑玉,等.精氨酸加压素和心钠素与高血压左室肥厚的关系.遵义医学院学报,2002,25(3)：209-10.
[7]江燕,廖圣宝.高血压神经内分泌免疫学研究进展.安徽医药,2010,14(6)：627-629.
[8]周全.细胞因子与高血压.医学综述,2006,12(7)：387-389.
[9]Cymerys M, Chyrek R, Bogdanski P, et al. Evaluation of acute phase proteins in hypertensive and obese patients. Pol Merkufiusz Lek,2003,15(88):352-355.
[10]Natarajan R, Rosdahl J, Gonzales N, et al. Regulation of 12-lipoxygenase by cytokine in ascular smooth muscle cells.Hypertension,1997,30(4):873-879.
[11]Huang G, Niu T, Peng S, et al. Association between the intedeukin-lbeta c(-5 11)T polymorphism and blood presure in Chinese hypertension population, Immunol Lettm.2004,91(2-3):159-162.
[12]Sesso HD, Buring JE, Rifai N, et al. C-reactive protein and the risk of developing hypertension. JAMA,2003,290:294-295.
[13]Bautista LE, Lopez-Jaramillo P. Is C-reactive protein an independent risk factor for essential hypertension. J Hypertens,2001,19:857-861.
[14]易筱荣,汪国琴.C反应蛋白与原发性高血压的关系.青海医药杂志,2009,39(8)：66-67.
[15]薛莉,刘泽军.高血压早期肾损害肾内血流动力学异常与内皮素及降钙素基因相关肽的关系.临床心血管病杂志,2009,25(5)：348-352.
[16]Deng PY, Li JY. Calcitonin gene-related peptide and hypertension.Peptides,2005, 26(9):1676.
[17]翁跃颂.血管活性肠肽对树突状细胞免疫调节作用研究进展.国际免疫学杂志,2006,29(2)：77-80.
[18]门琛,唐康庭,占伊扬.原发性高血压基因多态性的相关研究进展.医学综述,2010,16(17)：2569-2571.
[19]Hengtenberg C, Schunkert H, Mayer B, et al. Association between a polymorp Hism in the G-protein beta3 subunitgene with arterial hypertension but not with myocardial infarction. Cardiovasc Res,2001,49(4):820-827.
[20]Kleinz MJ, Davenport AP. Immun ocytochemical localization of the endogenous vasoactive peptide apelin to human vascular and endocardial endothelial cells. Regul Pept,2004,118(3):119-125.
[21]Zhong JC, Yu XY, Huang Y, et al. Apelin modulates aortic vascular tone via endothelial nitric oxide synthase phosphorylation pathway in diabetic mice. Cardiovase Res,2007, 74(3):388-395.
[22]Ishida J, Hashimoto T, Hashimoto Y, et al. Regulatory roles for APJ. A seven-transmembrane receptor related to angiotensin typel receptor in blood pressurein vivo. J Bio Chem,2004,279 (25):26274-26279.
[23]Saito Y, Nakamura T, Ohyama Y, et al. In vivo Klothogene delivery protects against endothelial dysfunction in multiple risk factor syndrome. Biochem Biophys Res Commun,2000,276(2):767-772.
[24]Suh 1, Nam CM, Kim SJ, et al. Association analysis of the essential hypertension susceptibility genes in adolescents:Kangwha study. J Prey Med Public Health,2006, 39(2):177-183.
[25]Kedzierska K. Ciechanowski K. Safranow K. et al. GNB3 C825T and ACE I/D polymorphisms on the sodium-proton exchanger and the prevalence of essential hypertension in males. Arch Med Res,2006,37(1):150-157.
[26]董一飞,慢性一氧化氮合成抑制模型及其产生高血压的机制.实用临床医学,2004,5(2)：132-133.
[27]吕琳,朱兴雷.一氧化氮在慢性充血性心力衰竭中的变化.临床医学,2000,20(3)：50-52.
[28]胡兴国,曾因明.NO/CGMP信号转导系统与全麻作用原理.《国外医学》麻醉学与复苏分册,1998,20(5)：269-271.
[29]Qiu C, Muchant D, Beierwaltes WH, et al. Evolution of Chronic Nitric Oxide Inhibition Hypertension:Relationship to Renal Function. Hypertension,1998,31 [part 1]:21-26.
[30]金松南,段瑞,文今福.NO-cGMP信号通路舒张甲亢性高血压大鼠胸主动脉的特性.中国药理学通报,2010,26(7)：910-914.
[31]陈冰,刘云兰,张鸿青.脑钠素与心血管系统疾病.中国实用医药,2007,2(24)：120-122.
[32]陈凯,汪海.血管内皮细胞非神经性乙酰胆碱系统的研究进展.中国临床药理学与治疗学,2005,10(3)：254-258.
[33]Kirkpatriek CJ, Bittinger F, Nozadze K, Wessler I. Expression and function of the non-neuronal cholinergic system in endothelial cells. Life Sci,2003,72:2111-2116.
[34]Maeklin KD, Maus AD, Pereira EF, Albuquerque EX. Human vascular endothelial cels express functional nicotinic acetylcholine receptors. J Pharmacol Exp,1998,287:435-439.
[35]KawashimaK, FujiiT. Basic and clinical aspects of non-neuronal acetylcholine:overview of non-neuronal cholinergic systems and their biological significance. J Pharmacol Sci, 2008,106(2):167-173.
[36]梁艺,钟国强.高脂血症与血管内皮功能异常研究进展.Internal Medicine of China, 2007,2(2):246-248.
[37]孙超.信号转导与人类疾病.北京：化学工业出版社,2006：8.
[38]李琦,欧阳静萍,余追.当归抗高脂致家兔主动脉粥样硬化效果观察.湖北中医学院学报,2003,5(2)：29-30.
[39]Beny J L, Nguyen M N, MarinoM, et a.l Muscarinic receptor knockoutmice confirm involvement ofM3receptor in endothelium-dependent vas dilatation in mouse arteries. J Cardiovasc Pharmacol,2008,51(5):505-512.
[40]李金菊,慕邵峰,曾淑君,等.内皮细胞乙酰胆碱靶标不同于M受体的药理学特征.中国药理学通报,2003,19(6)：660-664.
[41]Li J J,Mu S F, Zeng S J, et a.l Pharmacological characteristics of the endothelial-target for acetylcholine different from muscarinic receptors.Chin PharmacolBull,2003,19(6): 660-664.
[42]陈凯,汪海.激活内皮细胞非神经性M受体对不同亚型G蛋白基因表达的影响.中国药理学通报,2006,22(6)：664-7.
[43]郭益民,余立群,王颖,等.L-精氨酸对肾性高血压大鼠主动脉功能的保护作用.温州医学院学报,2006,36(2)：98-100.
[44]赵晓云,高宇,郭阳,等.内源性NO对糖尿病大鼠股动脉血压及血管反应性的影响.山东大学学报(医学版),2005,43(6)：536-538.
[45]都健,赵晓娟,刘国良.胰岛素抵抗大鼠内皮依赖性血管舒张功能研究.中华内分泌代谢杂志,2003,19(4)：313-315.
[46]刘磊,郑振声,钱学贤.高血压患者与正常血压者皮肤微血管对乙酰胆碱和硝普钠反应性比较.高血压杂志,2001,9(3)：187-188.
[47]刘娟娟,李森美,陈荣波.精神发育迟滞儿童血中乙酰胆碱质量浓度的研究.汕头大学医学院学报,1990,22(1)：36.
[48]陈世德,陶新智.一氧化氮的生理作用与心血管疾病关系的研究进展.蛇志,2002,14(1)：67-69.
[49]辜彦,王裕圯.妊高征肾病患者治疗前后血浆ET、CGRP和血清NO. NOS检测的临床意义.放射免疫学杂志,2009,22(3)：231-232.
[50]陈小波.一氧化氮与肾功能衰竭.华北煤炭医学院学报,2001,(1)：34.
[51]Toda N, Kitamura Y, OkamuraT. Neural mechanism of hypertension by nitric oxide synthase inhibitor in dogs.Hypertension,1993,21(3):3-8.
[52]段留法,郑秋甫,杨彦平,等.一氧化氮对自发性高血压大鼠血管内皮功能的作用.中华老年心脑血管病杂志,2000,2(1)：41-44.
[53]罗心平.一氧化氮对心功能影响的研究进展.国外医学心血管疾病分册,1998,25,5：271-274.
[1]王秀卿.慢性应激性高血压动物模型的建立.白求恩军医学院报,2003,1(4)：202-203.
[2]董艳芬,梁燕玲,罗艳,等.慢性应激诱发高血压大鼠模型的建立.解剖学研究,2006,28(1)：72-73.
[3]黄飞翔,游景成.高血压心脏损害的辨证论治.甘肃中医学院学报,2005,22(2)：12-13
[4]王风雷,张炜宁.张崇泉教授论治老年高血压病的经验摭拾.中医药学刊,2005,23(5)：793-796.
[5]费建平,王蕊,罗新民,等.温法治疗高血压病的临证拾零.光明中医,2006,21(9)：14-15.
[6]程志清,黄科.活血化瘀法治疗原发性高血压病的作用与地位.浙江中医学院学报,2004,28(1)：1-4.
[7]刘德桓,林惠琴,王秀宝,等.化瘀浊益肝肾法对原发性高血压患者生活质量的影响：随机双盲对照观察.中医临床康复,2006,10(23)：9-12.
[8]戴勇.两肾一夹肾性高血压大鼠模型的改进.实验动物科学与管理,2006,23(2)：60-62.
[9]廖圣宝.两肾一夹高血压大鼠模型中医证候属性的探讨及药物作用观察.中国中医基础医学杂志,2003,9(2)：34-37.
[10]Taska K.Anti hypertensive effects of nisoldipine and reference drugs in certain types of hypertensive rats.Arzneim-Forsch Drug Res,1987,37(1):316-317.
[11]李俊.高血压病中医动物模型研究现状与应用评价.广州中医药大学学报,2002,19(4)：321-322.
[12]肖纯.双肾双夹加灌附子汤法复制高血压肝阳上亢证大鼠模型.中国现代医学杂志,2000,10(9)：20-23.
[13]肖纯.潜阳方对高血压肝阳上亢证大鼠模型的实验研究.湖南中医学院学报,1999,19(2)：8-12.
[14]鄢东红.自发性高血压大鼠肝阳上亢证模型的复制.湖南中医学院学报,1999,19(4)：35-38.
[15]李海霞,杨惠芳.心理应激与高血压关联因素的研究进展.职业与健康,2009,25(8)：867-869.
[16]Poole JC, Snieder H, Davis HC,et al.Anger suppression and adiposity modulate association between ADRB2 haplotype and cardiovascular stress reactivity. PsychosomMed,2006,68 (2):207-212.
[17]Hamer M, Tanaka G,Okamura H, et al. The effects of depressive symptoms on cardiovascular and catecholamine responses to the induction of depressivemood. BiolPsychol,2007,74(1):20-25.
[18]LiMS, ZhaoH, Xia YH. Pregnanolone effects on the blood pressure of stress-induced hypertension in rats. Acta Physiol Sin,2004,56(4):471-475.
[19]Marshall GN, Sherbourne CD, Meredith LS,et al. The tripartite model of anxiety and depression:symptom structure in depressive and hypertensive patient groups.Pers Assess, 2003,80:139-153.
[20]谢雄伟,唐凤英,尤劲松,等.原发性高血压肝阳上亢证肝肾阴虚证的情绪测量比较.湖南中医学院学报,2003,23(6)：36-39.
[21]谢雄伟,尤昭玲.肝阳上亢证患者情绪状态特征临床研究.中医杂志,2006,47(11)：854-856.
[22]尤昭玲,谢雄伟,游卉.肝阳上亢证与相关证情绪状态测量的对比研究.湖南中医学院学报,2005,25(5)：1.
[23]郑庆玲,白玉山.应激性高血压动物模型的建立.福建医学院学报,1994,28(4)：424-425.
[24]顾红越.高血压中医辨证分型与血清B2-MG含量关系的探讨.浙江中医学院学报,1989,3(6)：25-19.
[25]徐贵成.平肝益肾法治疗Ⅰ期高血压的临床研究.北京中医杂志,1991,(6)：12-15.
[26]张兆蓬,王学员.针刺太冲穴治疗高血压病探讨.内蒙古中医药,2010,29(6)：129-130.
[27]吴焕林,李晓庆,王侠.针刺太冲穴对65例肝阳上亢型高血压病患者的即时降压效应.中医杂志,2008,49(7)：622-624.
[28]李晓庆,王侠,吴焕林.针刺太冲穴治疗肝阳上亢型高血压病的随机对照临床研究.辽宁中医杂志,2008,35(6)：919-920
[29]王侠,邹旭,李琼,等.针刺太冲穴治疗高血压的临床研究.实用医学杂志,2003,19(5)：565-566.
[30]汤飞雪,玛国湘,吴清明.针刺开四关加百会温针对原发性高血压患者血浆中ET和NO的影响.新中医,2004,1(24)：10-12.
[31]吴远华,朱广旗,林兴友等.针刺曲池太冲对高血压患者血压中ET和ACE的影响及疗效探讨.中国中西医结合杂志,2004,24(12)：1080.
[32]张红梅,刘晓炜,曲宏达.愤怒心理应激大鼠模型的建立及对行为／内分泌免疫的影响.中华精神科杂志,2005,38(2)：117-120.
[33]Kirkpatriek CJ, Bittinger F, Nozadze K, Wessler I. Expression and function of the non-neuronal cholinergic system in endothelial cells. Life Sci,2003,72:211-216.
[34]Macklin KD, MausAD, PereiraEF, Albuquerque EX. Human vascular endothelial cells express functional nicotinic acetylcholine receptors. J Pharmacol Exp Ther. 1998,287:435-439.
[35]Wessler I, Roth E, Deutsch C, Brickerhoff P,et al. Release of non-neuronal acetylcholine from the isolated human placenta is mediated by organic cation transporters. Br J Pharmacol,2001,134:951-956.
[36]Drexler H, Hayoz D, Munzel T, et al. Endothelial function in chronic congestive heart failure.Am J Cardiol.l992,69(19):1596-1601.
[37]Khurana S, Chacon I, Guofeng X, Yamada M, et al. Vasodilatory effects of cholinergic agonists are greatly diminished in aorta from M3R mice. Euro J Pharmacol, 2004,493:127-132.
[38]刘磊,郑振声,钱学贤.高血压患者与正常血压者皮肤微血管对乙酰胆碱和硝普钠反应性比较.高血压杂志,2001,9(3)：187-188.
[39]Undurti N. Acetylcholinesterase and butyrylcholinesterase as pos-sible markers of low-grade systemic inflammation. Med SciMonit,2007,13(12):RA214-221.
[40]钟慈声,孙安阳.一氧化氮的生物医学.上海科技文献出版社,1997：49-51.
[41]Radomski MW, Vallance P, Whitley G, er al. Platelet adhesion to human vascular endothelium is modulated by constitutive and cytokine induced nitric oxide. Cardiovasc Res,1993,27:1280-1382.
[42]Nugaguchi K, Egashira-K, Takemoto M, et al. Chronic inhibition of nitric oxide synthesis causes coronary microvascualr remodeling in rats. Hypertension, 1995,26:957-962.
[43]徐炳国,刘志诚.针刺对肥胖并发高血压患者一氧化氮和一氧化氮合酶含量的影响.浙江中医杂志,2006,41(3),134-136.
[44]吴清明,冯国湘,汤雪飞.针刺开“四关”加百会穴温针灸对原发性高血压患者血浆中ET和NO的影响.中国针灸,2004,24(1),53-56.
[45]王琴玉,唐巍.针刺对肾血管性高血压大鼠的降压效应.上海针灸杂志,2003,22(3)：6-8
[46]Toda N,Kitamura Y,OkamuraT.Neural mechanism of hypertension by nitric oxide synthase inhibitor in dogs.Hypertension,1993,21:3-8.
[47]段留法,郑秋甫,杨彦平,等.一氧化氮对自发性高血压大鼠血管内皮功能的作用.中华老年心脑血管病杂志,2000,2(1)：41-44.
[48]罗心平.一氧化氮对心功能影响的研究进展.国外医学心血管疾病分册,1998,25,5：271-274.
[49]孟智宏,杜元灏.针刺对实验性脑梗死大鼠脑心组织及血液cAMP. cGMP的影响.中国中医急症,2005,14(10)：989-991.
[50]张坚,刘晓春.针刺对缺血心肌内cAMP和cGMP含量的影响.针刺研究,1994,19(3)：108-109.
[51]周爽,刘又香.针刺对急性脑梗塞大鼠脑组织中cAMP,cGMP含量的影响.针灸临床杂志,2000,2(1)：13-15.
[52]张宏,唐纯志,陈永萍,等.电针至阳穴对急性心肌缺血家兔血浆cAMP、cGMP含量的影响.针灸临床杂志,2006,12(8)：687-688.
[53]吴旭平,孙国杰.电针“内关”对急性心肌缺血家兔延髓,心肌,血浆cAMP,cGMP含量的影响.针灸临床杂志,1999,15(9)：60-62.
[2]王苓,曾永蕾.“捻转”如何定“补泻”.针灸临床杂志,2001,17(11)：3-4.
[3]针灸甲乙经校释.山东中医学院校释.北京：人民卫生出版社,2009：546.
[4]许红君.从“北半球左漩涡”浅谈“捻转补泻法”.光明中医,2008,23(2)：166-167.
[5]孙星亮等.浅谈热补针法的起源及现状.陕西中医,2006,27(1)：90-91.
[6]卞金玲,张春红.石学敏院士针刺手法量学的概念及核心.中国针灸,2003,23(5)：287-289.
[7]郝应林.针刺“捻转补泻法”的探讨研究.世界中医药,2009,4(2)：102-104.
[8]文洪,王晓英.捻转补泻法与经脉循行方向的关系.针灸临床杂志,2005,21(5)：3-5.
[9]金百仁.捻转补泻探讨.浙江中医杂志,1991,26(2)：49-51.
[10]魏连海.重新认识捻转补泻针刺法.上海针灸杂志,2004,23(4)：13.
[11]睢明河,周宇姝,马文珠,等.大小刺激量捻转补泻法对血虚证大鼠红细胞计数的影响.针刺研究,2004,29(3)：213-216.
[12]董善京,孟丹.不同凉泻针刺手法对致热大鼠血浆内毒素水平影响的实验研究.河南中医药学刊,2001,16(6)：20-23.
[13]孙六合,董善京.凉泻手法对内毒素致热大鼠血清超氧化歧化酶水平的影响.时珍国医国药,2001,12(6)：546-549.
[14]方剑乔,林咸明,等.不同手法针刺对内毒素致热家兔体温的影响.针刺研究,1996,21(3)：50-52.
[15]王丽,王朝阳,支建梅,等.捻转补泻手法对应激性高血压大鼠颈交感神经放电的影响.北京中医药大学学报,2009,32(2)：142-145.
[16]王子梅,钱峰,陆纲,等.电刺激引发骨骼肌细胞钙震荡对nAChRy启动子活性的影响.中国生物化学与分子生物学报,2003,19(3)：283-285.
[17]关景芳,王理.针刺小鼠足三里穴组织化学实验观察.辽宁中医志,2002,29(6)：359-361.
[18]关卫,王芳.石氏捻转补泻手法对健康小鼠能量代谢相关酶的影响.天津中医,2002,19(5)：29-32.
[19]杨华元,钟小红,刘堂义,等.针刺仿真手法对高血压大鼠血压及心肌血管紧张素Ⅱ的影响.针刺研究,2008,33(3)：186-189.
[20]王君,张宝文,于新宇,等.疾徐捻转泻法针刺足三里对高血压家兔的降压作用.中国临床康复,2006,10(7)：116-117.
[21]邹移海,何智明,汤建华.捻转补泻手法对大鼠小肠系膜透明窗肥大细胞的影响.广东解剖学通报,1993,15(2)：107-111.
[22]郭永明.醋酸型胃溃疡大鼠PGE2变化及不同针刺手法的调节效应.辽宁中医杂志,2002,29(6)：313-315.
[23]王彩虹,王银平,许建敏,等.不同刺激量的捻转手法对健康人皮肤温度的影响.上海针灸杂志,2007,26(7)：33-35.
[24]王艳君,杨克涛.捻转补泻手法对健康人穴位皮肤温度的影响.中华实用中西医杂志,2003,3(16)：724-725.
[25]李平,关卫,等.捻转补泻手法针刺足三里穴对脘腹部皮肤温度的影响.天津中医学院学报,2002,19(4)：51-53.
[26]李平,关卫,等.捻转补泻手法针刺合谷穴对其局部皮肤温度的影响.天津中医学院学报,2002,21(3)：31-33.
[27]何智明.针刺补泻手法的实验研究.上海针灸杂志,1983,(2)：20-23.
[28]吕光,孙立明,李平.胆囊结石术后胃肠功能紊乱的针刺研究.陕西中医,2010,21(5)：587-589.
[29]许冠荪,郭原,张群群.针刺补泻手法对家兔实验性胃节律紊乱的影响.安徽中医学院学报,1992,11(2)：36-39.
[30]曹利民.浅谈针刺补泻手法.江苏中医,1993,14(8)：27-29.
[31]李万瑶.针刺提插补泻手法的胃电信息检测观察.中国针灸,1993,3(13)：29-32.
[32]李成宏,王玉中,郭新侠.针四关穴为主治疗原发性痛经临床观察.中国针灸,2008,28(3)：187-190.
[33]陶晓雁,孙彩霞,杨金亮.重刺激眼周腧穴治疗青少年近视临床观察.中国针灸,2008,28(3)：193-195.
[1]王舒,杨华,石学敏.国外原发性高血压的发病机制及诊治研究新进展.中西医结合心脑血管病杂志,2010,8(3)：342-344.
[2]张砀.血管活性肠肽的研究进展.医学综述,2009,15(21)：32-35.
[3]Schunkert H, Dzau V L Tang S S, et al. Increased rat cardiac angiotensin converting enzyme activity and m RNA expression in pressure overload left ventricular hypertrophy. Effects on Coronary resistance contractility, and relaxation. J Clin Invest,1990, 86(6):1913-1920.
[4]何昆仑,郑秋甫,牟善初,等.自发性高血压大鼠心肌肥大与心肌MAPK及AngⅡ的实验研究.解放军医学杂志,1998,23(2)：119-121.
[5]汪平,王宏.脑出血患者精氨酸加压素与水代谢相关性研究.中华神经外科疾病研究杂志,2009,8(4)：294-196.
[6]郝星,冼惠珍,马淑玉,等.精氨酸加压素和心钠素与高血压左室肥厚的关系.遵义医学院学报,2002,25(3)：209-10.
[7]江燕,廖圣宝.高血压神经内分泌免疫学研究进展.安徽医药,2010,14(6)：627-629.
[8]周全.细胞因子与高血压.医学综述,2006,12(7)：387-389.
[9]Cymerys M, Chyrek R, Bogdanski P, et al. Evaluation of acute phase proteins in hypertensive and obese patients. Pol Merkufiusz Lek,2003,15(88):352-355.
[10]Natarajan R, Rosdahl J, Gonzales N, et al. Regulation of 12-lipoxygenase by cytokine in ascular smooth muscle cells.Hypertension,1997,30(4):873-879.
[11]Huang G, Niu T, Peng S, et al. Association between the intedeukin-lbeta c(-5 11)T polymorphism and blood presure in Chinese hypertension population, Immunol Lettm.2004,91(2-3):159-162.
[12]Sesso HD, Buring JE, Rifai N, et al. C-reactive protein and the risk of developing hypertension. JAMA,2003,290:294-295.
[13]Bautista LE, Lopez-Jaramillo P. Is C-reactive protein an independent risk factor for essential hypertension. J Hypertens,2001,19:857-861.
[14]易筱荣,汪国琴.C反应蛋白与原发性高血压的关系.青海医药杂志,2009,39(8)：66-67.
[15]薛莉,刘泽军.高血压早期肾损害肾内血流动力学异常与内皮素及降钙素基因相关肽的关系.临床心血管病杂志,2009,25(5)：348-352.
[16]Deng PY, Li JY. Calcitonin gene-related peptide and hypertension.Peptides,2005, 26(9):1676.
[17]翁跃颂.血管活性肠肽对树突状细胞免疫调节作用研究进展.国际免疫学杂志,2006,29(2)：77-80.
[18]门琛,唐康庭,占伊扬.原发性高血压基因多态性的相关研究进展.医学综述,2010,16(17)：2569-2571.
[19]Hengtenberg C, Schunkert H, Mayer B, et al. Association between a polymorp Hism in the G-protein beta3 subunitgene with arterial hypertension but not with myocardial infarction. Cardiovasc Res,2001,49(4):820-827.
[20]Kleinz MJ, Davenport AP. Immun ocytochemical localization of the endogenous vasoactive peptide apelin to human vascular and endocardial endothelial cells. Regul Pept,2004,118(3):119-125.
[21]Zhong JC, Yu XY, Huang Y, et al. Apelin modulates aortic vascular tone via endothelial nitric oxide synthase phosphorylation pathway in diabetic mice. Cardiovase Res,2007, 74(3):388-395.
[22]Ishida J, Hashimoto T, Hashimoto Y, et al. Regulatory roles for APJ. A seven-transmembrane receptor related to angiotensin typel receptor in blood pressurein vivo. J Bio Chem,2004,279 (25):26274-26279.
[23]Saito Y, Nakamura T, Ohyama Y, et al. In vivo Klothogene delivery protects against endothelial dysfunction in multiple risk factor syndrome. Biochem Biophys Res Commun,2000,276(2):767-772.
[24]Suh 1, Nam CM, Kim SJ, et al. Association analysis of the essential hypertension susceptibility genes in adolescents:Kangwha study. J Prey Med Public Health,2006, 39(2):177-183.
[25]Kedzierska K. Ciechanowski K. Safranow K. et al. GNB3 C825T and ACE I/D polymorphisms on the sodium-proton exchanger and the prevalence of essential hypertension in males. Arch Med Res,2006,37(1):150-157.
[26]董一飞,慢性一氧化氮合成抑制模型及其产生高血压的机制.实用临床医学,2004,5(2)：132-133.
[27]吕琳,朱兴雷.一氧化氮在慢性充血性心力衰竭中的变化.临床医学,2000,20(3)：50-52.
[28]胡兴国,曾因明.NO/CGMP信号转导系统与全麻作用原理.《国外医学》麻醉学与复苏分册,1998,20(5)：269-271.
[29]Qiu C, Muchant D, Beierwaltes WH, et al. Evolution of Chronic Nitric Oxide Inhibition Hypertension:Relationship to Renal Function. Hypertension,1998,31 [part 1]:21-26.
[30]金松南,段瑞,文今福.NO-cGMP信号通路舒张甲亢性高血压大鼠胸主动脉的特性.中国药理学通报,2010,26(7)：910-914.
[31]陈冰,刘云兰,张鸿青.脑钠素与心血管系统疾病.中国实用医药,2007,2(24)：120-122.
[32]陈凯,汪海.血管内皮细胞非神经性乙酰胆碱系统的研究进展.中国临床药理学与治疗学,2005,10(3)：254-258.
[33]Kirkpatriek CJ, Bittinger F, Nozadze K, Wessler I. Expression and function of the non-neuronal cholinergic system in endothelial cells. Life Sci,2003,72:2111-2116.
[34]Maeklin KD, Maus AD, Pereira EF, Albuquerque EX. Human vascular endothelial cels express functional nicotinic acetylcholine receptors. J Pharmacol Exp,1998,287:435-439.
[35]KawashimaK, FujiiT. Basic and clinical aspects of non-neuronal acetylcholine:overview of non-neuronal cholinergic systems and their biological significance. J Pharmacol Sci, 2008,106(2):167-173.
[36]梁艺,钟国强.高脂血症与血管内皮功能异常研究进展.Internal Medicine of China, 2007,2(2):246-248.
[37]孙超.信号转导与人类疾病.北京：化学工业出版社,2006：8.
[38]李琦,欧阳静萍,余追.当归抗高脂致家兔主动脉粥样硬化效果观察.湖北中医学院学报,2003,5(2)：29-30.
[39]Beny J L, Nguyen M N, MarinoM, et a.l Muscarinic receptor knockoutmice confirm involvement ofM3receptor in endothelium-dependent vas dilatation in mouse arteries. J Cardiovasc Pharmacol,2008,51(5):505-512.
[40]李金菊,慕邵峰,曾淑君,等.内皮细胞乙酰胆碱靶标不同于M受体的药理学特征.中国药理学通报,2003,19(6)：660-664.
[41]Li J J,Mu S F, Zeng S J, et a.l Pharmacological characteristics of the endothelial-target for acetylcholine different from muscarinic receptors.Chin PharmacolBull,2003,19(6): 660-664.
[42]陈凯,汪海.激活内皮细胞非神经性M受体对不同亚型G蛋白基因表达的影响.中国药理学通报,2006,22(6)：664-7.
[43]郭益民,余立群,王颖,等.L-精氨酸对肾性高血压大鼠主动脉功能的保护作用.温州医学院学报,2006,36(2)：98-100.
[44]赵晓云,高宇,郭阳,等.内源性NO对糖尿病大鼠股动脉血压及血管反应性的影响.山东大学学报(医学版),2005,43(6)：536-538.
[45]都健,赵晓娟,刘国良.胰岛素抵抗大鼠内皮依赖性血管舒张功能研究.中华内分泌代谢杂志,2003,19(4)：313-315.
[46]刘磊,郑振声,钱学贤.高血压患者与正常血压者皮肤微血管对乙酰胆碱和硝普钠反应性比较.高血压杂志,2001,9(3)：187-188.
[47]刘娟娟,李森美,陈荣波.精神发育迟滞儿童血中乙酰胆碱质量浓度的研究.汕头大学医学院学报,1990,22(1)：36.
[48]陈世德,陶新智.一氧化氮的生理作用与心血管疾病关系的研究进展.蛇志,2002,14(1)：67-69.
[49]辜彦,王裕圯.妊高征肾病患者治疗前后血浆ET、CGRP和血清NO. NOS检测的临床意义.放射免疫学杂志,2009,22(3)：231-232.
[50]陈小波.一氧化氮与肾功能衰竭.华北煤炭医学院学报,2001,(1)：34.
[51]Toda N, Kitamura Y, OkamuraT. Neural mechanism of hypertension by nitric oxide synthase inhibitor in dogs.Hypertension,1993,21(3):3-8.
[52]段留法,郑秋甫,杨彦平,等.一氧化氮对自发性高血压大鼠血管内皮功能的作用.中华老年心脑血管病杂志,2000,2(1)：41-44.
[53]罗心平.一氧化氮对心功能影响的研究进展.国外医学心血管疾病分册,1998,25,5：271-274.
[1]王秀卿.慢性应激性高血压动物模型的建立.白求恩军医学院报,2003,1(4)：202-203.
[2]董艳芬,梁燕玲,罗艳,等.慢性应激诱发高血压大鼠模型的建立.解剖学研究,2006,28(1)：72-73.
[3]黄飞翔,游景成.高血压心脏损害的辨证论治.甘肃中医学院学报,2005,22(2)：12-13
[4]王风雷,张炜宁.张崇泉教授论治老年高血压病的经验摭拾.中医药学刊,2005,23(5)：793-796.
[5]费建平,王蕊,罗新民,等.温法治疗高血压病的临证拾零.光明中医,2006,21(9)：14-15.
[6]程志清,黄科.活血化瘀法治疗原发性高血压病的作用与地位.浙江中医学院学报,2004,28(1)：1-4.
[7]刘德桓,林惠琴,王秀宝,等.化瘀浊益肝肾法对原发性高血压患者生活质量的影响：随机双盲对照观察.中医临床康复,2006,10(23)：9-12.
[8]戴勇.两肾一夹肾性高血压大鼠模型的改进.实验动物科学与管理,2006,23(2)：60-62.
[9]廖圣宝.两肾一夹高血压大鼠模型中医证候属性的探讨及药物作用观察.中国中医基础医学杂志,2003,9(2)：34-37.
[10]Taska K.Anti hypertensive effects of nisoldipine and reference drugs in certain types of hypertensive rats.Arzneim-Forsch Drug Res,1987,37(1):316-317.
[11]李俊.高血压病中医动物模型研究现状与应用评价.广州中医药大学学报,2002,19(4)：321-322.
[12]肖纯.双肾双夹加灌附子汤法复制高血压肝阳上亢证大鼠模型.中国现代医学杂志,2000,10(9)：20-23.
[13]肖纯.潜阳方对高血压肝阳上亢证大鼠模型的实验研究.湖南中医学院学报,1999,19(2)：8-12.
[14]鄢东红.自发性高血压大鼠肝阳上亢证模型的复制.湖南中医学院学报,1999,19(4)：35-38.
[15]李海霞,杨惠芳.心理应激与高血压关联因素的研究进展.职业与健康,2009,25(8)：867-869.
[16]Poole JC, Snieder H, Davis HC,et al.Anger suppression and adiposity modulate association between ADRB2 haplotype and cardiovascular stress reactivity. PsychosomMed,2006,68 (2):207-212.
[17]Hamer M, Tanaka G,Okamura H, et al. The effects of depressive symptoms on cardiovascular and catecholamine responses to the induction of depressivemood. BiolPsychol,2007,74(1):20-25.
[18]LiMS, ZhaoH, Xia YH. Pregnanolone effects on the blood pressure of stress-induced hypertension in rats. Acta Physiol Sin,2004,56(4):471-475.
[19]Marshall GN, Sherbourne CD, Meredith LS,et al. The tripartite model of anxiety and depression:symptom structure in depressive and hypertensive patient groups.Pers Assess, 2003,80:139-153.
[20]谢雄伟,唐凤英,尤劲松,等.原发性高血压肝阳上亢证肝肾阴虚证的情绪测量比较.湖南中医学院学报,2003,23(6)：36-39.
[21]谢雄伟,尤昭玲.肝阳上亢证患者情绪状态特征临床研究.中医杂志,2006,47(11)：854-856.
[22]尤昭玲,谢雄伟,游卉.肝阳上亢证与相关证情绪状态测量的对比研究.湖南中医学院学报,2005,25(5)：1.
[23]郑庆玲,白玉山.应激性高血压动物模型的建立.福建医学院学报,1994,28(4)：424-425.
[24]顾红越.高血压中医辨证分型与血清B2-MG含量关系的探讨.浙江中医学院学报,1989,3(6)：25-19.
[25]徐贵成.平肝益肾法治疗Ⅰ期高血压的临床研究.北京中医杂志,1991,(6)：12-15.
[26]张兆蓬,王学员.针刺太冲穴治疗高血压病探讨.内蒙古中医药,2010,29(6)：129-130.
[27]吴焕林,李晓庆,王侠.针刺太冲穴对65例肝阳上亢型高血压病患者的即时降压效应.中医杂志,2008,49(7)：622-624.
[28]李晓庆,王侠,吴焕林.针刺太冲穴治疗肝阳上亢型高血压病的随机对照临床研究.辽宁中医杂志,2008,35(6)：919-920
[29]王侠,邹旭,李琼,等.针刺太冲穴治疗高血压的临床研究.实用医学杂志,2003,19(5)：565-566.
[30]汤飞雪,玛国湘,吴清明.针刺开四关加百会温针对原发性高血压患者血浆中ET和NO的影响.新中医,2004,1(24)：10-12.
[31]吴远华,朱广旗,林兴友等.针刺曲池太冲对高血压患者血压中ET和ACE的影响及疗效探讨.中国中西医结合杂志,2004,24(12)：1080.
[32]张红梅,刘晓炜,曲宏达.愤怒心理应激大鼠模型的建立及对行为／内分泌免疫的影响.中华精神科杂志,2005,38(2)：117-120.
[33]Kirkpatriek CJ, Bittinger F, Nozadze K, Wessler I. Expression and function of the non-neuronal cholinergic system in endothelial cells. Life Sci,2003,72:211-216.
[34]Macklin KD, MausAD, PereiraEF, Albuquerque EX. Human vascular endothelial cells express functional nicotinic acetylcholine receptors. J Pharmacol Exp Ther. 1998,287:435-439.
[35]Wessler I, Roth E, Deutsch C, Brickerhoff P,et al. Release of non-neuronal acetylcholine from the isolated human placenta is mediated by organic cation transporters. Br J Pharmacol,2001,134:951-956.
[36]Drexler H, Hayoz D, Munzel T, et al. Endothelial function in chronic congestive heart failure.Am J Cardiol.l992,69(19):1596-1601.
[37]Khurana S, Chacon I, Guofeng X, Yamada M, et al. Vasodilatory effects of cholinergic agonists are greatly diminished in aorta from M3R mice. Euro J Pharmacol, 2004,493:127-132.
[38]刘磊,郑振声,钱学贤.高血压患者与正常血压者皮肤微血管对乙酰胆碱和硝普钠反应性比较.高血压杂志,2001,9(3)：187-188.
[39]Undurti N. Acetylcholinesterase and butyrylcholinesterase as pos-sible markers of low-grade systemic inflammation. Med SciMonit,2007,13(12):RA214-221.
[40]钟慈声,孙安阳.一氧化氮的生物医学.上海科技文献出版社,1997：49-51.
[41]Radomski MW, Vallance P, Whitley G, er al. Platelet adhesion to human vascular endothelium is modulated by constitutive and cytokine induced nitric oxide. Cardiovasc Res,1993,27:1280-1382.
[42]Nugaguchi K, Egashira-K, Takemoto M, et al. Chronic inhibition of nitric oxide synthesis causes coronary microvascualr remodeling in rats. Hypertension, 1995,26:957-962.
[43]徐炳国,刘志诚.针刺对肥胖并发高血压患者一氧化氮和一氧化氮合酶含量的影响.浙江中医杂志,2006,41(3),134-136.
[44]吴清明,冯国湘,汤雪飞.针刺开“四关”加百会穴温针灸对原发性高血压患者血浆中ET和NO的影响.中国针灸,2004,24(1),53-56.
[45]王琴玉,唐巍.针刺对肾血管性高血压大鼠的降压效应.上海针灸杂志,2003,22(3)：6-8
[46]Toda N,Kitamura Y,OkamuraT.Neural mechanism of hypertension by nitric oxide synthase inhibitor in dogs.Hypertension,1993,21:3-8.
[47]段留法,郑秋甫,杨彦平,等.一氧化氮对自发性高血压大鼠血管内皮功能的作用.中华老年心脑血管病杂志,2000,2(1)：41-44.
[48]罗心平.一氧化氮对心功能影响的研究进展.国外医学心血管疾病分册,1998,25,5：271-274.
[49]孟智宏,杜元灏.针刺对实验性脑梗死大鼠脑心组织及血液cAMP. cGMP的影响.中国中医急症,2005,14(10)：989-991.
[50]张坚,刘晓春.针刺对缺血心肌内cAMP和cGMP含量的影响.针刺研究,1994,19(3)：108-109.
[51]周爽,刘又香.针刺对急性脑梗塞大鼠脑组织中cAMP,cGMP含量的影响.针灸临床杂志,2000,2(1)：13-15.
[52]张宏,唐纯志,陈永萍,等.电针至阳穴对急性心肌缺血家兔血浆cAMP、cGMP含量的影响.针灸临床杂志,2006,12(8)：687-688.
[53]吴旭平,孙国杰.电针“内关”对急性心肌缺血家兔延髓,心肌,血浆cAMP,cGMP含量的影响.针灸临床杂志,1999,15(9)：60-62.