破血化瘀填精补髓法对实验性脑出血大鼠血清S100B蛋白的作用研究
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摘要
目的：本实验通过研究“破血化瘀、填精补髓法”对实验性脑出血大鼠神经功能改善、血肿吸收以及血清S100B蛋白水平的影响，探讨“破血化瘀、填精补髓法”治疗脑出血的作用机制，为其临床应用提供可靠的实验学依据。
方法：成年健康Wistar大鼠，雌雄各半，体重250g～300g左右，共126只，随机分成6组，即正常组，假手术组，模型组，破血化瘀、填精补髓法高中低剂量组各1组，正常组6只，其余每组各24只，后5组再随机分为术后1d、3d、7d、14d共4个时间点，每组每个时间点6只。采用自体血注入法建立大鼠脑出血模型。正常组自由饮食饮水；假手术组和模型组分别用生理盐水以1ml/100g/d体重灌胃；治疗低剂量组以0.5ml/100g/d汤药灌胃；中、高剂量组分别以低剂量的2倍和4倍给药。观察各组大鼠神经功能缺损评分，并在相应时间点腹主动脉取血约4ml，离心，取上清，测定S100B蛋白浓度。取血后灌注取脑，用相机拍照，用病理图象分析软件Image-Pro Plus计算血肿面积百分比。
结果：大鼠脑出血模型建立后第3天和7天时，破血化瘀填精补髓法高中低剂量组神经功能缺损评分、血肿面积百分比以及血清S100B蛋白水平较模型组有显著差异（P＜0.05），而高剂量组又较中低剂量组有显著下降（P＜0.05），且血清S100B蛋白水平与神经功能缺损评分变化趋势一致。说明破血化瘀填精补髓中药可以降低神经功能损伤的程度、促进血肿的吸收以及降低血清S100B蛋白浓度。
结论：破血化瘀填精补髓法指导下的中药可以改善脑出血大鼠神经功能缺损程度，促进血肿的吸收以及降低血清S100B蛋白浓度，具有保护神经组织的作用；脑出血后血清S100B蛋白水平与神经功能缺损程度具有相关性；高剂量的破血化瘀填精补髓方药作用效果更明显。
Purpose: Based on the study of neurological recovery, hematoma and serum S100Bprotein levels of experimental intracerebral hemorrhage in rats,the experiment discisses themechanism of “removing blood stasis and replenishing essence and marrow”of the treatmentof ICH,and provides a reliable experim-ental science basis for clinical therapy.
Method: Healthy adult Wistar rats,male and female,weighing250g～300g or so,a totalof126.The rats are randomly divided into six groups:normal group,sham operation group,ICHmodel group,high、medium and low dose treatment group.Normal group has six rats,theremaining five group have24rats,which were randomly divided into four time points,such as1d,3d,7d,14d of postoperation of ICH,and it has6rats in each group at each time point.Weuse autologous blood injection method to make an intracerebral hemorrhage model inrats.Rats of the normal group are freely allowed to eating and drinking water.Rats of shamcontrol operation group and model group were poured1ml physiological saline into stomachper100g every day.Rats of low-dose group were poured0.5ml decoction into stomach per100g every day,rats of medium-dose and high-dose group were given2and4times the doseof low-dose group.We observe neurological deficit score of rats in each group,and extractabdominal aortic blood about4ml at the corresponding time points,centrifuge,extractserum,determine concentration of S100protein.After extracting blood,we perfuse brain troughthe heart,take out the brain,and calculate the hematoma volume with the pathological imageanalysis software Image-Pro Plus after taking pictures with the camera.
Result: Making the intracerebral hemorrhage model, high, medium and low doses of“removing blood stasis and replenishing essence and marrow”of neurological deficit score,the percentage of the hematoma area and serum S100B protein levels were significantly lowerthan model group (P<0.05). And high dose group was significantly decreased than themedium and low dose group (P<0.05). The trend of serum S100B protein levels andneurological deficit scores is consistent. Traditional Chinese medicine of “removing bloodstasis and replenishing essence and marrow”can reduce the degree of neurological deficit, andpromote the absorption of hematoma and reduce the concentration of serum S100B protein.
Conclusion: Traditional Chinese medicine of“removing blood stasis and replenishingessence and marrow” can improve degree of neurological deficits,promote the absorption ofhematoma and reduce the concentration of serum S100B protein.It has a role to protect thenerve tissue.Serum S100B protein levels and degree of neurological deficits have correlationafter intracerebral hemorrhage. High dose group of“removing blood stasis and replenishingessence and marrow"have the most obvious effect.
方法：成年健康Wistar大鼠，雌雄各半，体重250g～300g左右，共126只，随机分成6组，即正常组，假手术组，模型组，破血化瘀、填精补髓法高中低剂量组各1组，正常组6只，其余每组各24只，后5组再随机分为术后1d、3d、7d、14d共4个时间点，每组每个时间点6只。采用自体血注入法建立大鼠脑出血模型。正常组自由饮食饮水；假手术组和模型组分别用生理盐水以1ml/100g/d体重灌胃；治疗低剂量组以0.5ml/100g/d汤药灌胃；中、高剂量组分别以低剂量的2倍和4倍给药。观察各组大鼠神经功能缺损评分，并在相应时间点腹主动脉取血约4ml，离心，取上清，测定S100B蛋白浓度。取血后灌注取脑，用相机拍照，用病理图象分析软件Image-Pro Plus计算血肿面积百分比。
结果：大鼠脑出血模型建立后第3天和7天时，破血化瘀填精补髓法高中低剂量组神经功能缺损评分、血肿面积百分比以及血清S100B蛋白水平较模型组有显著差异（P＜0.05），而高剂量组又较中低剂量组有显著下降（P＜0.05），且血清S100B蛋白水平与神经功能缺损评分变化趋势一致。说明破血化瘀填精补髓中药可以降低神经功能损伤的程度、促进血肿的吸收以及降低血清S100B蛋白浓度。
结论：破血化瘀填精补髓法指导下的中药可以改善脑出血大鼠神经功能缺损程度，促进血肿的吸收以及降低血清S100B蛋白浓度，具有保护神经组织的作用；脑出血后血清S100B蛋白水平与神经功能缺损程度具有相关性；高剂量的破血化瘀填精补髓方药作用效果更明显。
Purpose: Based on the study of neurological recovery, hematoma and serum S100Bprotein levels of experimental intracerebral hemorrhage in rats,the experiment discisses themechanism of “removing blood stasis and replenishing essence and marrow”of the treatmentof ICH,and provides a reliable experim-ental science basis for clinical therapy.
Method: Healthy adult Wistar rats,male and female,weighing250g～300g or so,a totalof126.The rats are randomly divided into six groups:normal group,sham operation group,ICHmodel group,high、medium and low dose treatment group.Normal group has six rats,theremaining five group have24rats,which were randomly divided into four time points,such as1d,3d,7d,14d of postoperation of ICH,and it has6rats in each group at each time point.Weuse autologous blood injection method to make an intracerebral hemorrhage model inrats.Rats of the normal group are freely allowed to eating and drinking water.Rats of shamcontrol operation group and model group were poured1ml physiological saline into stomachper100g every day.Rats of low-dose group were poured0.5ml decoction into stomach per100g every day,rats of medium-dose and high-dose group were given2and4times the doseof low-dose group.We observe neurological deficit score of rats in each group,and extractabdominal aortic blood about4ml at the corresponding time points,centrifuge,extractserum,determine concentration of S100protein.After extracting blood,we perfuse brain troughthe heart,take out the brain,and calculate the hematoma volume with the pathological imageanalysis software Image-Pro Plus after taking pictures with the camera.
Result: Making the intracerebral hemorrhage model, high, medium and low doses of“removing blood stasis and replenishing essence and marrow”of neurological deficit score,the percentage of the hematoma area and serum S100B protein levels were significantly lowerthan model group (P<0.05). And high dose group was significantly decreased than themedium and low dose group (P<0.05). The trend of serum S100B protein levels andneurological deficit scores is consistent. Traditional Chinese medicine of “removing bloodstasis and replenishing essence and marrow”can reduce the degree of neurological deficit, andpromote the absorption of hematoma and reduce the concentration of serum S100B protein.
Conclusion: Traditional Chinese medicine of“removing blood stasis and replenishingessence and marrow” can improve degree of neurological deficits,promote the absorption ofhematoma and reduce the concentration of serum S100B protein.It has a role to protect thenerve tissue.Serum S100B protein levels and degree of neurological deficits have correlationafter intracerebral hemorrhage. High dose group of“removing blood stasis and replenishingessence and marrow"have the most obvious effect.
引文
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