香蕉果实特异表达cDNA及果实特异表达启动子的分离鉴定
|
摘要
香蕉和大蕉是热带、亚热带发展中国家的最重要作物之一。是全球约四亿人的日常食物,香蕉已成为世界第四大粮食作物。香蕉贸易是产蕉国家及地区换取外汇的主要方式。特别是发展中国家,从中获取85%,目前香蕉已是许多发展中国家农业的重要组成部分,正迅速发展。中国的香蕉产量居世界第四位,是热带农业的支柱产业。香蕉为典型的呼吸跃变型水果,果实采后不耐储藏。对其采后生理学及分子生物学研究已成为研究热点,目的在于深入了解该类果实后熟机理,为延长果实保鲜期提供理论依据及为创造保鲜新方法奠定基础。
微阵列是目前功能基因组学研究的重要手段之一,该方法可高通量、快速筛选目的基因,同时可研究基因在发育过程中的表达情况。我们首次利用此方法对通过抑制差减法获得的287个香蕉采后两天果实的cDNA片段进行了不同器官的表达研究,通过计算机自动扫描及软件分析,共获得32个果实特异的cDNA片段。进行Blastn和Blastx比较分析,结果表明获得32个果实特异表达cDNA片段,包括信号转导(4个)、成熟相关蛋白(4个)、蛋白激酶(3个)及物质代谢(9个)等相关基因。根据芯片扫描光吸收值得大小,选取大、中、小的三个克隆(编号BR24、BR47、BR63)进行Northern blot分析,结果证明三个克隆均为果实特异表达,表达量也与芯片一致。选取编号BR63的克隆利用RACE方法获得5′端500bp,3′端1800bp。测序结果表明该cDNA全长2443bp,含有一个完整的阅读框架,长1959bp,编码652个氨基酸。氨基酸同源性比较(BlastX)与小麦、水稻的淀粉磷酸化酶基因(α-1,4-葡聚糖磷酸化酶)同源72%。结合香蕉采后成熟过程中乙烯释放量及呼吸强度测定变化特点,选取采后成熟不同时间段的果实提取RNA反转录cDNA,利用RT-PCR方法对各时间段mRNA表达进行检测,结果表明,在采收前(0h)未有表达,采收后随着成熟度提高表达量逐渐下降。说明该基因的表达是在呼吸跃变之前,而在跃变开始时,已很少或不表达。
栽培香蕉基本是三倍体,常规育种较为困难。近年来随着生物技术,特别是转基因技术的不断成熟及香蕉再生体系的不断完善,利用生物技术培育香蕉新品种已成共识。同时由于香蕉自身的特点,将其作为生物反应器生产口服疫苗及有用的化合物也成研究的热点。外源基因特异高效表达,启动子的作用十分重要,香蕉为单子叶植物,
Bananas and plantains (Musa spp) are globally important fruit crop, especially in developing tropic and sub-tropic countries, because they feed tens of millions in Central America and Asia. Besides that, banana trade is an essential source of income for many national economies. Therefore, the United Nations Food and Agriculture Organization ranks bananas as the world's fourth most important food crop. Banana postharvest ripening plays an important role in influencing fruit quality and shelf life. As a result, many researches on molecular biological and physical mechanism of banana postharvest ripening have been carried out. Banana fruits are typical climacteric fruits, with many features in ripening like tomatoes, avocado, mango and others. Many experiments demonstrate that banana fruit ripening is regulated by ethylene, companied by aspiratory climacteric. In the view of molecular biology, protein de novo synthesis is necessary for many of physiological changes during postharvest. For the reason of that, by cloning the transcripts of the proteins related to fruit ripening, it would be possible to understand the genes related to fruit ripening and their expression patterns.Differential screening of cDNA libraries representing ripening banana pulp led to the isolation of groups of differential expressed mRNAs. Identification of these transcripts partial sequence analysis indicates that proteins encoded by these genes involved in ethylene biosynthesis, aspiration, starch mobilization, cell wall breakdown and other mobilizations. In this study, cDNA microarrays combined with suppression subtractive hybridizaiton (SSH) were carried out successfully for isolating fruit-specific expressed cDNA. 287 cDNA fragments from SSH were printed onto a glass plate so that a cDNA chip was made. By using labeled RNA from root, leaf and stem, and fruit as probes to screen the chip, as a form of reverse Northern blot, 32 cDNA clones were identified as fruit-specific expressed in banana. Sequencing of these 32 cDNAs showed that 4 cDNAs
were homology to those genes related to signal transduction, 4 clones were homology with those genes encoding ripening associated protein, 3 clones were homology with those genes encoding protein kinase, 9 clones were homology with those genes encoding some enzymes which took part in some metabolisms in banana fruit ripening, 8 clones were homology with those genes encoding different proteins with different functions and also 4 clones had no homologues in NCBI. The fruit-specific expressed cDNA in the chip were confirmed to be fruit-specific by using Northern blot, in which 3 clones selected randomly were used as probes to hybridize to RNA from root, stem and leaf, and fruit. The results of Northern blot suggested that those cDNAs expressed not only specially in fruit, but also the quantity of expression were just similar to those in the chip. It is the first report that so many clones have been isolated from banana and so important cDNA have been obtained, because just total 36 clones had been obtained previously.The gene encoding a -1,4-glucan phosphorylase has been firstly cloned in this study. As for the importance in banana fruit ripening, the full length of the gene was obtained by using rapid amplification of cDNA 5' and 3' end and RT-PCR was used to examine the expression patterns in different organs and different stages of fruit ripening. In order to determine the ripening stages, ethylene biosynthesis and CO_2 production in postharvest banana were measured. Combining the result of RT-PCR with different ripening stages represented by ethylene biosynthesis and CO_2 production, it could be conclude that a -1,4-glucan phosphorylase gene expression happened much earlier than the bust of ethylene biosynthesis and CO_2 production. However, the function of this gene in fuit ripening was still needed to further reseach.A highly fruit-specific expressed promoter takes an important role in expressing foreign protein in fruit, which make banana be possible to be used as bioreactor to produce oral pharmaceutical proteins and chemicals in this way. For the reason of that a highly fruit-specific expressed gene promoter called BanLec gene promoter was cloned in this study. It was demonstrated tha BanLec gene expressed with a very high level in banana fruit in our previous reseach. BanLec gene promoter was isolated by using chromosome walking and a 670 nt DNA with basic elements of promoter, such as TATA-box, CAAT-box,
微阵列是目前功能基因组学研究的重要手段之一,该方法可高通量、快速筛选目的基因,同时可研究基因在发育过程中的表达情况。我们首次利用此方法对通过抑制差减法获得的287个香蕉采后两天果实的cDNA片段进行了不同器官的表达研究,通过计算机自动扫描及软件分析,共获得32个果实特异的cDNA片段。进行Blastn和Blastx比较分析,结果表明获得32个果实特异表达cDNA片段,包括信号转导(4个)、成熟相关蛋白(4个)、蛋白激酶(3个)及物质代谢(9个)等相关基因。根据芯片扫描光吸收值得大小,选取大、中、小的三个克隆(编号BR24、BR47、BR63)进行Northern blot分析,结果证明三个克隆均为果实特异表达,表达量也与芯片一致。选取编号BR63的克隆利用RACE方法获得5′端500bp,3′端1800bp。测序结果表明该cDNA全长2443bp,含有一个完整的阅读框架,长1959bp,编码652个氨基酸。氨基酸同源性比较(BlastX)与小麦、水稻的淀粉磷酸化酶基因(α-1,4-葡聚糖磷酸化酶)同源72%。结合香蕉采后成熟过程中乙烯释放量及呼吸强度测定变化特点,选取采后成熟不同时间段的果实提取RNA反转录cDNA,利用RT-PCR方法对各时间段mRNA表达进行检测,结果表明,在采收前(0h)未有表达,采收后随着成熟度提高表达量逐渐下降。说明该基因的表达是在呼吸跃变之前,而在跃变开始时,已很少或不表达。
栽培香蕉基本是三倍体,常规育种较为困难。近年来随着生物技术,特别是转基因技术的不断成熟及香蕉再生体系的不断完善,利用生物技术培育香蕉新品种已成共识。同时由于香蕉自身的特点,将其作为生物反应器生产口服疫苗及有用的化合物也成研究的热点。外源基因特异高效表达,启动子的作用十分重要,香蕉为单子叶植物,
Bananas and plantains (Musa spp) are globally important fruit crop, especially in developing tropic and sub-tropic countries, because they feed tens of millions in Central America and Asia. Besides that, banana trade is an essential source of income for many national economies. Therefore, the United Nations Food and Agriculture Organization ranks bananas as the world's fourth most important food crop. Banana postharvest ripening plays an important role in influencing fruit quality and shelf life. As a result, many researches on molecular biological and physical mechanism of banana postharvest ripening have been carried out. Banana fruits are typical climacteric fruits, with many features in ripening like tomatoes, avocado, mango and others. Many experiments demonstrate that banana fruit ripening is regulated by ethylene, companied by aspiratory climacteric. In the view of molecular biology, protein de novo synthesis is necessary for many of physiological changes during postharvest. For the reason of that, by cloning the transcripts of the proteins related to fruit ripening, it would be possible to understand the genes related to fruit ripening and their expression patterns.Differential screening of cDNA libraries representing ripening banana pulp led to the isolation of groups of differential expressed mRNAs. Identification of these transcripts partial sequence analysis indicates that proteins encoded by these genes involved in ethylene biosynthesis, aspiration, starch mobilization, cell wall breakdown and other mobilizations. In this study, cDNA microarrays combined with suppression subtractive hybridizaiton (SSH) were carried out successfully for isolating fruit-specific expressed cDNA. 287 cDNA fragments from SSH were printed onto a glass plate so that a cDNA chip was made. By using labeled RNA from root, leaf and stem, and fruit as probes to screen the chip, as a form of reverse Northern blot, 32 cDNA clones were identified as fruit-specific expressed in banana. Sequencing of these 32 cDNAs showed that 4 cDNAs
were homology to those genes related to signal transduction, 4 clones were homology with those genes encoding ripening associated protein, 3 clones were homology with those genes encoding protein kinase, 9 clones were homology with those genes encoding some enzymes which took part in some metabolisms in banana fruit ripening, 8 clones were homology with those genes encoding different proteins with different functions and also 4 clones had no homologues in NCBI. The fruit-specific expressed cDNA in the chip were confirmed to be fruit-specific by using Northern blot, in which 3 clones selected randomly were used as probes to hybridize to RNA from root, stem and leaf, and fruit. The results of Northern blot suggested that those cDNAs expressed not only specially in fruit, but also the quantity of expression were just similar to those in the chip. It is the first report that so many clones have been isolated from banana and so important cDNA have been obtained, because just total 36 clones had been obtained previously.The gene encoding a -1,4-glucan phosphorylase has been firstly cloned in this study. As for the importance in banana fruit ripening, the full length of the gene was obtained by using rapid amplification of cDNA 5' and 3' end and RT-PCR was used to examine the expression patterns in different organs and different stages of fruit ripening. In order to determine the ripening stages, ethylene biosynthesis and CO_2 production in postharvest banana were measured. Combining the result of RT-PCR with different ripening stages represented by ethylene biosynthesis and CO_2 production, it could be conclude that a -1,4-glucan phosphorylase gene expression happened much earlier than the bust of ethylene biosynthesis and CO_2 production. However, the function of this gene in fuit ripening was still needed to further reseach.A highly fruit-specific expressed promoter takes an important role in expressing foreign protein in fruit, which make banana be possible to be used as bioreactor to produce oral pharmaceutical proteins and chemicals in this way. For the reason of that a highly fruit-specific expressed gene promoter called BanLec gene promoter was cloned in this study. It was demonstrated tha BanLec gene expressed with a very high level in banana fruit in our previous reseach. BanLec gene promoter was isolated by using chromosome walking and a 670 nt DNA with basic elements of promoter, such as TATA-box, CAAT-box,
引文
[1] J.萨姆布鲁克,E.F.弗里奇,T.曼尼阿蒂斯著;金冬雁,黎梦枫译。分子克隆试验指南(第2版)。科学出版社,2002
[2] 陈雷,杨福全,张天佑,韩雅珊,陈尚武.虎杖中白藜芦醇和白藜芦醇甙的高速逆流色谱分离提纯及分析.分析测试学报,2000,19(4):60-62
[3] 陈绍荣,吕应堂,杨弘远.磷酸化酶mRNA在水稻雌蕊中表达的时空动态.遗传,1999,21(3):31～33
[4] 傅荣昭,孙勇如,贾士荣.植物遗传转化技术手册.北京:中国科学技术出版社,1994,140-142
[5] 胡廷章.植物激素效应启动子.生命的化学,2000,20(3)120-122
[6] 胡位荣,朱西儒,王正询等。香蕉果实采后及储藏生理研究进展。广州大学学报自然科学版),2003,2(3):228-234
[7] 华南农业大学主编,果树栽培学各论(南方本),农业出版社(第二版)1993,P104
[8] 惠特斯勒,R.L.,J.N.贝密勒,B.F.帕斯卡尔合编。淀粉的化学及工艺学。王维文,闵大铨,杨家雄等译。北京:中国食品出版社,1988,8:95-101
[9] 贾春平,曾溢滔。增强子作用机制的研究进展。生命科学,2002,14(2):73-76
[10] 金志强,彭世清,孔德骞,郑学勤,李建国.香蕉后熟过程中乙烯的生物合成及其与ACC含量的关系.热带作物学报,1995,16(增刊):26-30
[11] 李丽,张景昱,杜桂森。种子特异启动子(NapinBPromoter)分离、表达载体构建及转基因植株获得。植物学通报,2001,18(2):216-220
[12] 廖慧敏,高强,刘谋益。基因芯片技术及其在植物上的应用。湖南农业科学,2003,5:19-22
[13] 刘军,袁自强,刘建东,钱晓茵,钱曼,杨金水.水稻幼穗发育早期特异表达的基因[J].科学通报,2000,45(13):1392-1397
[14] 吕嵩,黄晓琳.DNA芯片技术原理及在遗传学研究中的应用.国外医学遗传学分册,1998,21(6):284-289
[15] 毛自朝,于秋菊,甄伟,郭俊益,胡鸢雷,高音,林忠平.果实专一性启动子驱动ipt基因在番茄中的表达及其对番茄果实发育的影响.科学通报,2002,47(6): 444-447
[16] 门福义,毛雪飞,刘梦云等,马铃薯不同品种淀粉积累生理基础研究——淀粉积累与磷酸化酶。马铃薯杂志,1997,第11卷,第1期:1-6
[17] 石东乔,周奕华,万里红。甘蓝型油菜BcNA1基因启动子在转基因烟草中对Gus基因表达的调控。植物生理学报,2001,27(4):313-320
[18] 苏伟,硕士论文,香蕉采后果实成熟特异表达基因的分离。华南热带农业大学,2003年6月,海口
[19] 孙彤,闫莉婕,黄勤妮,印丽萍.缺铁水稻根转录本微阵列分析.生物信息学,2003、4(3):1-5
[20] 谭林.芪合酶基因转化番茄产生白藜芦醇的研究。华南热带农业大学博士论文,2002年5月,海口
[21] 王关林,方宏筠主编.植物基因工程(第二版).科学技术出版社,2002
[22] 王贵禧,韩雅珊,于梁。猕猴桃总淀粉酶活性与果实软化的关系。园艺学报,1994,21:(4):329-333
[23] 王景雪,孙毅,徐培林。植物功能基因组学研究进展。生物技术通报,2004,1:18-22
[24] 王念捷,程奇,任延国,章力建,沈桂芳.水稻4CL基因启动子引导的GUS在紫外诱导下的组织特异性表达.中国农业科学,1996,29(1):73-77
[25] 王新力,彭学贤.香蕉果实成熟相关基因ACO1启动子区的克隆及其功能初探.生物工程学报,2001,17(4):428-431.
[26] 王新力,彭学贤.香蕉果实特异性ACC合酶基因启动子区的克隆及其功能初探.生物工程学报,2001,17(3):293-296.
[27] 王颖、麦维军、梁承邺,张明永.高等植物启动子的研究进展.西北植物学报,2003,23(11):2040-2048
[28] 王永胜 王景 李发强 刘筱斌 刘良式.SSH法获取水稻矮化突变体相关的cDNA片段.高技术通讯2001.5:20-24
[29] 肖洁凝,黄学林,黄霞,李筱菊.芒果生长素反应因子类蛋白的cDNA克隆和表达.生物工程学报,2004,20(1):59～62
[30] 徐碧玉,刘歌,金志强.香蕉Actin1启动子的分离及启动活性的分析。热带作物学报,2005,2,已接受。
[31] 徐昌杰,陈昆松,张上隆。气调对猕猴桃果实储藏的效应及其生理基础。应用基础与工程科学学包,1998,6(2):134-139
[32] 许志茹,李玉花,杨传平。基因芯片技术在拟南芥研究中的应用。生物技术通迅,2004,5:509-512
[33] 杨予涛,杨国栋,刘石娟郭兴启,郑成超.一个光合组织特异表达强启动子的分离和功能分析.中国科学C辑,2003,33(4):298-306
[34] 于玲,王莱,牛吉山,陈佩度.植物功能基因组研究进展.西北师范大学学报(自然科学版),2003,1:104-116
[35] 曾骧主编.果树生理学,北京农业大学出版社,1992,第一版,P237-238
[36] 张得有、徐碧玉、金志强.香蕉果胶裂解酶基因的克隆.植物生理与分子生物学报.2003,29(5):401-404.
[37] 庄军平.香蕉果实成熟软化相关生理及分子基础的研究.华南农业大学,博士论文,2004年6月,广州
[38] Adrian M, Jeandet P, Veneau J.. Biological activity of resveratrol, a stilbenic compound from grapevines, against Botrytis cinerea, the causal agent for gray mold. J Chem Ecol. 1997,23:1689-1702.
[39] Agravante J U, Matsui T, Kitagawa H. Starch breakdown in ethylene-treated and ethanol-treated bananas: changes in phosphorylase and invertased activities during ripening. J. Jpn Soc Food Sci. Technol, 1990.37:911-915.
[40] Agravante J U, Matsui T, Kitagawa H. Changes in pectin-methylesterase polygalacturonase and pecic substances of ethanol-treated bananas during ripening. J. Jpn Soc FoodSci. Technol, 1991. 38:527-532.
[41] Aharoni A, Keizer L C P, Bouwmeester H J, Sun Z, Alvarez-Huerta M, Verhoeven H A, Blaas J, van Houwelingen M M L, De Vos R C H, van der Voet H. Identification of the SAAT gene involved in strawberry flavor biogenesis by use of DNA microarray. Plant Cell, 2000,12:647-661
[42] Annadana S, Beekwilderm J, Kuipers G. Cloning of the chrysanthemum UEP1 promoter and comparative expression in florets and leaves of Dendranthema frandiflora. Transgenic Res, 2002, 11(4):437-445
[43] Atkinson R G, Bolitho K M, Wright M A, Bueno T I, Reid S J, Ross G S. Apple ACC oxidase and polygalacturonase ripening-specific gene expression and promoter analysis in transgenic tomato. Plant Mol. Biol. 1995,28:423-435
[44]Watson M A, Perry A, Budhjara V, Hicks C, Shannon W D, Rich K M. Gene expression profiling with oligonucleotide microarrays disting gliomas. Cancer Res,2001,61(5): 1825-1829
[45]. M Bahn S C, Bac M S, Park Y B, Oh S I, Jeung J U, Bae J M, Chung Y S, Shin J S. Molecular cloning and characterization of a novel low temperature-induced gene, blti2, from barley (Hordeumvulgare L.) Biochim. Biophys Acta,2001,1522(2): 134-137
[46]Ballas N, Wong LM, Ke M, Theologis A. Two auxin-responsive domains interact positively to induce expression of the early indoleacetic acid-inducible gene PS-IAA4/5. Proc NatlAcadSci U S A. 1995, 92(8):3483-7
[47]Barre A, Peumans W J, Menu-Bouaouiche L, Els Van Damme J M, May G D, Herrera A F, Van Leuven F, Rouge P. Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit. Planta, 2000,211: 791-799.
[48]Becker D, Brettschneider R, Lorz H. Fertile transgenic Wheat from microprojectile bombardment of scutellar tissue. The plant Journal,1994,5(2):299-307
[49]Bertelli AAE, Giobannini L, Giannessi D, Migliori M, Bernini W, Fregoni M, Bertelli A. Antiplatelet activity of synthetic and natural resveratrol in red wine, Int J Tissue React 1995,17:1-3.
[50]Bonihi C, Pagni S, Vidrih R. Cell wall hydrolases and amylase in kiwifruir softening. Posrharvest Biology and Technology. 1996,9(1): 19-29
[51]Buzel R A, Cascardo J C, Rodrigues L A. Tissue-spcific regulation of BiP gene:a cis-acting regulatory domain is required for BiP promoter activity in plant meristems. Plant Mol. Biol. 2002,50(4-5):757-771.
[52] Chee M, Yang R, Hubbell E, Berno A, Huang XC, Stern D, Winkler J, Lockhart DJ, Morris MS, Fodor SP. Accessing genetic information with high-density DNA arrays. Science, 1996,274:610-614
[53] Chengappa S, Guilleroux M, Phillips W, Shields R. Transgenic tomato plants with decreased sucrose synthase are unaltered in starh and sugar accumulation in the fruit. Plant Mol. Biol. 1990,40:213-221
[54]Ching-Yi Wan, Thea A. Wilkins, 1994, A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton(Gossypium hirsutum L.) Analytical. Biochemistry 223,7-12
[55]Clendennen S K, Kipp P B, May G D. The role of ethylene in banana fruit ripening. In AK Kanellis, C Chang, H Kende, D Grierson, eds, Biology and Biotechnology of the Plant Hormone Ethylene. Kluwer Academic Publishers, Dordrecht, The Netherlands,1997a,115: 141-148.
[56]Clendennen S K, Lopez-Gomez R, Gomez-Lim M, Arntzen C J, May G D, The abundant 31-kilodalton banana pulp protein is homologous to classⅢ-acidic chitinase. Phytochenistry, 1998,47: 613-619.
[57]Clendennen S K, May G D. Differential gene expression in ripening banana fruit. Plant Physiol, 1997b,115:463-469.
[58]Clontech PCR-Select? cDNASubtraction Kit. 1996, CLONTECHniques\A-%
[59]Cordenunsi B R, Lajolo F M, Starch breakdown during banana ripening: sucrose synthase and sucrose phosphate synthase. J Agric Food Chem, 1995.43:347-351.
[60]Cordenunsi B R, Lajolo F M, Starch breakdown during banana ripening: sucrose synthase and sucrose phosphate synthase. J Agric Food Chem, 1995.43:347-351.
[61]CSIRO Division of Food Research. The ripening banana. ANANA standard colour index numbers for banana ripening. Melbourne:CSIRO 1985
[62]Deikman J, Xu RL, Kneissl ML, Ciardi JA, Kim KN, Pelah D. Separation of cis elements responsive to ethylene, fruit development, and ripening in the 5'-flanking region of the ripening-related E8 gene. Plant Molecular Biology 1998,37:1001-1011
[63] Diatchenko L, Lau Y F, Campbll A P, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD. Suppression subtractive hybridization:A method for generatiog differentially regulated or tissue-specific cDNA probes and libraries. Proc Notl Acad Sci, 1996,93:6025-6030
[64]Dolferus R, Jacobs M, Peacock WJ, Dennis ES. Differential interactions of promoter elements in stress responses of the Arabidopsis Adh gene. Plant Physiol. 1994 Aug;105(4):1075-87. [65]Dominguez-Puigjaner E, Immaculada L, Vendrell M, and Prat S. A cDNA clone highly expressed in ripe banana fruit shows homology to pectate lyases. Plant Physiol,1997,114:1071-1076
[66]Dominguez-Puigjaner E, Vendrell M, Ludevid D M. Differential protein accumulation in banana fruit during ripening. Plant Physiol, 1992,98:157-162.
[67]Dugdale B, Becker DK, Marding RM. Intron-mediated enchancement of the banana bunchy top virus DNA-6 promoter in banana(Musa spp.)embryogenic cells and planes. Plant Cell Reports.,,2001,,20:220-226
[68] Dugdale B,Beetham PR, Becker DK, Harding RM, Dale JL. Promoter activity associated with the intergenic regions of banana bunchy top virus DNA-1 to-6 in transgenic tobacco and banana cells. J Gen Virol, 1998,79:2301-2311
[69]Fodor SPA, Read J L, Huang X L. Multiples biochemical assaya with biological chips. Nature, 1993,364(6437):555-556
[70] Fodor SPA, Read J L, Pinung M, Stryer L, Lu AT, Solas D. Light- directed, spatially addressable parallel chemical synthesis. Science,1991,251(4995):767-773M
[71]Frankel EN, Waterhouse AL, Kinsella JE . Inhibition of human LDL oxidation by resveratrol. Lancet. 1993,24:1103-1104.
[72]Fukuda Y. Interaction of tobacco nuclear protein with an elicitor-responsive in the promoter of a basic class I chitinase gene. Plant Mol Biol 1997,34(1):81
[73]Gooding P S, Bird C, Robinson S P. Molecular cloning and characterization of banana fruit polyphenol oxidase. Plant, 2001,213:748-757
[74]Gubler and J. V. Jacobsen. Gibberellin-Responsive Elements in the Promoter of a Barley High-pl [alpha]-Amylase Gene . Plant Cell .1992,4: 1435-1441.
[75]Gurskaya N K, Diatchenko L, Chenckik A, Siebert PD, Khaspekov GL, Lukyanov KA, Vagner LL, Ermolaeva OD, Lukyanov SA, Sverdlov ED. Equalization cDNA subtraction based on selective suppression of ploymerase chain reaction; ckoning of Jurkat cell transcripts induced by phytohemaglutinin and phlrbol 12-myristate 13-ad. Analytical Biochemistry. 1996,240:90-97
[76] Hain R, Biesler B, Kindl H, Schroder G, Stocker R. Expression of a stilbene synthase gene in Nicotiana tabacum results in synthesis of phytoaiexin reveratrol. Plant Mol Biol. 1990,15:325-335.
[77]Hain R, Reif H-J, Krause E, Langebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E, Schreier PH, Stacker RH. Disease resistance results from foreign phytoaiexin expression in a novel plant. Nature .1993,361:153-156.
[78]Hermann SR, Harding RM, Dale JI. The banana actin 1 promoter drices near-constitutive transgene expression in vegetative tissues of banana(Musa spp.). Plant Cell Reports,2001,20:525-530
[79]Hill S, ap Rees T, The effect of glucose on the control of carbohydrate metabolism in ripening bananas. Planta. 1995b. 196:335-343.
[80]Hill S, ap Rees T, The effect of hypoxia on the control of carbohydrate metabolism in ripening bananas.Planta. 1995a. 197:313-323.
[81] Horsch RB, Fry JE, Hoffman N L, Eichholtz D, Rogers S G, Fraley R T. A simple and general method for transferring genes into plants. Science, 1985,227:1229-1231
[82]Huang P L, Do Y Y, Huang F C, Thay T S, Chang T W. Characterization and expression analysis of a banana gene encoding 1-aminocyclopropane-l-carboxylate oxidase. Biochem Mol Bio Int. 1997. 41:941-950.
[83]Ingram J, Bartels D. The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:377-403.
[84]Iwasaki T., Yamaguchi-Shinozaki K., and Shinozaki K. Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis. Mol. Gen. Genet. 1995,247,391-398.
[85]Iyare O A, Ekwukoma B O. Changes in the activities of carbohydrate-degrading enzymes with ripening in Musa paradisiaca. J. Sci Food Agric. 1992.58:173-176.
[86]Jiang M, Cai L, Udeani GO et al, Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science. 1997, 275-278.
[87]Jin Zhi-Qiang, ZHANG De-You, XU Bi-Yu. Cloning and Developmental and Tissue-Specific Expression of Banana Lectin Gene. Acta Genetica Sinica. 2004,31(5):508-512
[88]Jin Zhiqiang, Zhang Deyou, Xu Biyu. Using genomic DNA as standard for competitive RT-PCR to quantify BanLec gene expression in ripening banana. Research and development of tropical biological resources. Proceedings of the international workshop on research and development of tropical biological resources.2002,10. P.67-70.
[89]Jorda L, Vera P. Local and systemic induction of two defense-related subtilisin-like protease promoters in teansgenic Arabidopsis plant.Luciferin induction of PR fene expression. Plant Physiol.2000,124(3):1049-1058
[90]Josh G. DNA sequencers to go bananas? Science 2001 293: 585-586
[91]Kavaler E, Landman J, Chang Y, et al.. Detecting human bladder carcinoma cells in voided urine samples by assaying for the presence of telomerase activity. Cancer.1998,82(4);708-714
[92] Kim J Y, Chung Y S, Paek K H, Park Y I, Kim J K, Yu S N, Oh B J, Shin. Isolation and characterization of a cDNA encoding the cysteine proteinase inhibitor, induced upon flower maturation in carnction using suppression subtractive bybridization. Mol Cells, 1999,9(4):392-397
[93]Kimura Y, Okuda H, Arichi S . Effects of stilbenes on arachidonate metabolism in leukocytes. Biochim Biophys Acta . 1985,834:275-278.
[94]Kobayashi, S., Ding, CK, Nakamura, Y, Nakajima, I. and Matsumoto, R., Kiwifruit. (Actinida deliciosa) transformed with a Vitis stilbene synthase gene produce piceid. (resveratrol-glucoside). Plant Cell Rep. 2000,19: 904-910.
[95]Koch KE. Carbohydrate-modulated gene expression in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1996,41:509~540
[96]Kojima K, Sakurai N, Kuraishi S, Fruit softening in banana: correlation among stress-relaxation parameters, cell wall components and starch during ripening. Physiol Plant. 1994.90:772-778.
[97]Leckband G, Lorz H . Transformation and expression of a stilbene synthase gene of Vitis vinifera L in barley and wheat for increased fungal resistance. Theor Aappl Genet 1998,96:1004-1012
[98]Lee J M, William M E. Tingey S V, et al., DNA array profiling of fene expression changes during maize embryo development. Funct Integr Genomics. 2002,2:13-27
[99]Li-jia Qu. Jun Chen, Meihua Liu.et al., Molecular cloning and functional analysis of a NoveType of Bowman-Birk inhibitor gene family in Rice. Plant Physiology. 2003,133:560-570
[100]Liu X, Shiomi S, Nakatsuka A, Kubo Y, Nakamura R, Inaba A. Characterization of ethylene biosynthesis associated with ripening in banana fruit. Plant Physiol,1999121:1257-1265.
[101] Lockhart D J,Dong H, Byrne M C, Follettie MT, Gallo MV, Chee MS, Mittmann M, Wang C, Kobayashi M, Horton H, Brown EL. Expression monitoring by hybridization high-density oligonucleotide arrays. Nature Biote. 1996,14:1675-1680
[102]L6pez-G6mez R, Campbell A, Dong J G, Yang S F, Gomez-Lim M A. Ethylene biosynthesis in banana fruit: isolation of a genomic clone to ACC oxidase and expression studies. Plant Sci. 1997. 123: 123-131.
[103]Lutcke H A, Chow K C, Mickel F S. Selection of AUG in itiation codons differs in plants andanimals. The Embo. Journal, 1987,6:43-48
[104]Mariaa A C, Concepci N, Terry L. Differential regulation of small heat-shock gene in plants: analysis of a water-stress-inducible and developmentally activated sunflower promoter. Plant Molecular Biology. 1996,31:863-876
[105]Medina-Suarez R, Manning K, Fletcher J, Aked J Rird C R, Seymour G B, Gene expression in the pulp of ripening bananas. Plant Physiol, 1997.115:453-461.
[106]Mendoza E M T, Laurena A G, Uritani I, Astringency and polyphenols in banana. In I Uritani, VV Garcia, EMT Mendoza, eds, Posthavest Biochemistry of Plant Food-Materials in the Tropics. Japan Scientific Societies Press, Tokyo, 1994. p.177-191.
[107]Michel D, Salamini F, Bartels D, Dale P, Baga M, Szalay A. Analysis of a desiccation and ABA-responsive promoter isolated from the resurrection plant Craterostigma plantagineum. Plant J. 1993. 4(l):29-40.
[108]Nagao RT, Goekjian VH, Hong JC, Key JL. Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean. Plant Mol Biol. 1993 21(6):1147-62.
[109] Nanjo T, Fujita M, Seki M, Kato T, Tabata S, Shinozaki K. Toxicity of free proline revealed in a Arabidopsis T-DNA-Tagged mutant deficient in proline dehydrogenase. Plant Cell Physiol.2003,44:541
[110]Nascimento J R O, Cordenunsi B R, Lajolo F M, Alcocer M J C, Banana sucrose-phosphate synthase gene expression during fruit ripening. Planta, 1997. 203:283-288.
[111] Negishi T, Nakanishi H, Yazakin J, Kishimoto N, Fujii F, Shimbo K, Yamamoto K, Sakata K, Sasaki T, Kikuchi S, Mori S, Nishizawa NK. cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests thar polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots. Plant J,2002,30(l):83-94
[112]Nicole Schupp, Paul Ziegler. The relation of starch phosphorylases to starch metabolism in wheat. Plant Cell physiol.2004,45(10):1471-1484
[113]Robinson N J, Procter C M, Connolly E L, Guerinot M L. A ferric-chelate reductase for iron uptake from soils. Nature. 1999,397:694-697
[114]Olive MR, Peacock WJ, Dennis ES. The anaerobic responsive element contains two GC-rich sequences essential for binding a nuclear protein and hypoxic activation of the maize Adhl promoter. Nucleic Acids Res. 1991. 19(25):7053-60.
[115] Pace-Asciak CR, Hahn S, Diamandis EP, Soleas G, Goldberg DM. The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: implications for protection against coronary heart desease. Clin Chim Acta 1995,235:207-219.
[116]Penarrubia L, Kim R, Giovannoni J. Production of the sweet protein monellin in transgenic plants. Bio/Technol. 1992,10:561-564
[117] Penarrubia L, Kim R, Giovannoni J, Kim S H, Fisher R. Production of the sweet protein monellin in transgenic plants. Bio/Technol. 1992,10:561-564
[118]Peter K. Busk and Montserrat Pages. Regulation of abscisic acid-induced transcription. Plant Molecular Biology. 1998,37(3):425-435
[119]Peumans W J, Zhang W, Barre A, Astoul C H, Balint-Kurti P J, Rovira P, Rouge P, May G D, Van Leuven F, Truffa-Bachi P, Van Damme E J M, Fruit-specific lectins from banana and plantain. Planta, 2000. 211:546-554.
[120]Pingyi Zhang, Roy L. Whistler, James N. BeMiller, Bruce R. Hamaker*. Banana starch: production, physicochemical properties,and digestibility—a review. Carbohydrate Polymers 59 (2005) 443-458
[121]Pua E C, Lee Y C, Expression of a ripening-related cytochrome P450 cDNA in Cavendish banana (Musa acuminata cv. Williams). Gene, 2003. 305:133-40
[122]Ralf P, Fritz S. Heat shock elements are involved in heat shock promoter activation during tobacco seed maturation. Plant Molecular Biology. 1996,31:157-162
[123]Reilly T P, Bourdi M, Brady JN, et al., Expression profiling of acetam inophen liver toxicity in mice using microarray technology. Biochem Biophys Res Common,2001,282(l):321-328
[124]Reymond P, Weber H, Damond M, et al., Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. Plant Cell,2000,12:707-719
[125]Robinson D J, Environmental risk assessment of release of transgenic plants containing virus-derived inserts. Transgen Res. 1996,5:359-362
[126]Ruan Y J, Gimore J, Onner T. Toward Arobidopsis genome analysis: monitoring expression profile of 1400 genes using cDNA microarrays. Plant J. 1998,15(6);821
[127]Samson JA., Tropical Fruits, London:longman, 1980,P119
[128]Schena M, Shalon D, Drvis R, et al., Quantitative monitoring of gene expression patterns with acomplementary DNA microarray. Science,1995,270(5235):467-470
[129]Schena M, Shalon D, Helle R,et al., Parallel human genome analysis: microarray-based expression monitorin of 1000 genes. Proc Natl Acad Sci USA.1996,93(20):10614-10619
[130]Schenk PM, Sagi LS. Remans T. Dietzgen RG. Bermard MJ. Graham MW. Manners JMA. Promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants. Plant Mol Biol, 1999,39:1221-1230
[131]Seki M, Narusaka M, Abe H. et al., Monitoring the expression pattern of 1300 Arabidopsis genes under draught and cold stresses by using a full-length cDNA microarray. Plant Cell.2001,13:61
[2] 陈雷,杨福全,张天佑,韩雅珊,陈尚武.虎杖中白藜芦醇和白藜芦醇甙的高速逆流色谱分离提纯及分析.分析测试学报,2000,19(4):60-62
[3] 陈绍荣,吕应堂,杨弘远.磷酸化酶mRNA在水稻雌蕊中表达的时空动态.遗传,1999,21(3):31～33
[4] 傅荣昭,孙勇如,贾士荣.植物遗传转化技术手册.北京:中国科学技术出版社,1994,140-142
[5] 胡廷章.植物激素效应启动子.生命的化学,2000,20(3)120-122
[6] 胡位荣,朱西儒,王正询等。香蕉果实采后及储藏生理研究进展。广州大学学报自然科学版),2003,2(3):228-234
[7] 华南农业大学主编,果树栽培学各论(南方本),农业出版社(第二版)1993,P104
[8] 惠特斯勒,R.L.,J.N.贝密勒,B.F.帕斯卡尔合编。淀粉的化学及工艺学。王维文,闵大铨,杨家雄等译。北京:中国食品出版社,1988,8:95-101
[9] 贾春平,曾溢滔。增强子作用机制的研究进展。生命科学,2002,14(2):73-76
[10] 金志强,彭世清,孔德骞,郑学勤,李建国.香蕉后熟过程中乙烯的生物合成及其与ACC含量的关系.热带作物学报,1995,16(增刊):26-30
[11] 李丽,张景昱,杜桂森。种子特异启动子(NapinBPromoter)分离、表达载体构建及转基因植株获得。植物学通报,2001,18(2):216-220
[12] 廖慧敏,高强,刘谋益。基因芯片技术及其在植物上的应用。湖南农业科学,2003,5:19-22
[13] 刘军,袁自强,刘建东,钱晓茵,钱曼,杨金水.水稻幼穗发育早期特异表达的基因[J].科学通报,2000,45(13):1392-1397
[14] 吕嵩,黄晓琳.DNA芯片技术原理及在遗传学研究中的应用.国外医学遗传学分册,1998,21(6):284-289
[15] 毛自朝,于秋菊,甄伟,郭俊益,胡鸢雷,高音,林忠平.果实专一性启动子驱动ipt基因在番茄中的表达及其对番茄果实发育的影响.科学通报,2002,47(6): 444-447
[16] 门福义,毛雪飞,刘梦云等,马铃薯不同品种淀粉积累生理基础研究——淀粉积累与磷酸化酶。马铃薯杂志,1997,第11卷,第1期:1-6
[17] 石东乔,周奕华,万里红。甘蓝型油菜BcNA1基因启动子在转基因烟草中对Gus基因表达的调控。植物生理学报,2001,27(4):313-320
[18] 苏伟,硕士论文,香蕉采后果实成熟特异表达基因的分离。华南热带农业大学,2003年6月,海口
[19] 孙彤,闫莉婕,黄勤妮,印丽萍.缺铁水稻根转录本微阵列分析.生物信息学,2003、4(3):1-5
[20] 谭林.芪合酶基因转化番茄产生白藜芦醇的研究。华南热带农业大学博士论文,2002年5月,海口
[21] 王关林,方宏筠主编.植物基因工程(第二版).科学技术出版社,2002
[22] 王贵禧,韩雅珊,于梁。猕猴桃总淀粉酶活性与果实软化的关系。园艺学报,1994,21:(4):329-333
[23] 王景雪,孙毅,徐培林。植物功能基因组学研究进展。生物技术通报,2004,1:18-22
[24] 王念捷,程奇,任延国,章力建,沈桂芳.水稻4CL基因启动子引导的GUS在紫外诱导下的组织特异性表达.中国农业科学,1996,29(1):73-77
[25] 王新力,彭学贤.香蕉果实成熟相关基因ACO1启动子区的克隆及其功能初探.生物工程学报,2001,17(4):428-431.
[26] 王新力,彭学贤.香蕉果实特异性ACC合酶基因启动子区的克隆及其功能初探.生物工程学报,2001,17(3):293-296.
[27] 王颖、麦维军、梁承邺,张明永.高等植物启动子的研究进展.西北植物学报,2003,23(11):2040-2048
[28] 王永胜 王景 李发强 刘筱斌 刘良式.SSH法获取水稻矮化突变体相关的cDNA片段.高技术通讯2001.5:20-24
[29] 肖洁凝,黄学林,黄霞,李筱菊.芒果生长素反应因子类蛋白的cDNA克隆和表达.生物工程学报,2004,20(1):59～62
[30] 徐碧玉,刘歌,金志强.香蕉Actin1启动子的分离及启动活性的分析。热带作物学报,2005,2,已接受。
[31] 徐昌杰,陈昆松,张上隆。气调对猕猴桃果实储藏的效应及其生理基础。应用基础与工程科学学包,1998,6(2):134-139
[32] 许志茹,李玉花,杨传平。基因芯片技术在拟南芥研究中的应用。生物技术通迅,2004,5:509-512
[33] 杨予涛,杨国栋,刘石娟郭兴启,郑成超.一个光合组织特异表达强启动子的分离和功能分析.中国科学C辑,2003,33(4):298-306
[34] 于玲,王莱,牛吉山,陈佩度.植物功能基因组研究进展.西北师范大学学报(自然科学版),2003,1:104-116
[35] 曾骧主编.果树生理学,北京农业大学出版社,1992,第一版,P237-238
[36] 张得有、徐碧玉、金志强.香蕉果胶裂解酶基因的克隆.植物生理与分子生物学报.2003,29(5):401-404.
[37] 庄军平.香蕉果实成熟软化相关生理及分子基础的研究.华南农业大学,博士论文,2004年6月,广州
[38] Adrian M, Jeandet P, Veneau J.. Biological activity of resveratrol, a stilbenic compound from grapevines, against Botrytis cinerea, the causal agent for gray mold. J Chem Ecol. 1997,23:1689-1702.
[39] Agravante J U, Matsui T, Kitagawa H. Starch breakdown in ethylene-treated and ethanol-treated bananas: changes in phosphorylase and invertased activities during ripening. J. Jpn Soc Food Sci. Technol, 1990.37:911-915.
[40] Agravante J U, Matsui T, Kitagawa H. Changes in pectin-methylesterase polygalacturonase and pecic substances of ethanol-treated bananas during ripening. J. Jpn Soc FoodSci. Technol, 1991. 38:527-532.
[41] Aharoni A, Keizer L C P, Bouwmeester H J, Sun Z, Alvarez-Huerta M, Verhoeven H A, Blaas J, van Houwelingen M M L, De Vos R C H, van der Voet H. Identification of the SAAT gene involved in strawberry flavor biogenesis by use of DNA microarray. Plant Cell, 2000,12:647-661
[42] Annadana S, Beekwilderm J, Kuipers G. Cloning of the chrysanthemum UEP1 promoter and comparative expression in florets and leaves of Dendranthema frandiflora. Transgenic Res, 2002, 11(4):437-445
[43] Atkinson R G, Bolitho K M, Wright M A, Bueno T I, Reid S J, Ross G S. Apple ACC oxidase and polygalacturonase ripening-specific gene expression and promoter analysis in transgenic tomato. Plant Mol. Biol. 1995,28:423-435
[44]Watson M A, Perry A, Budhjara V, Hicks C, Shannon W D, Rich K M. Gene expression profiling with oligonucleotide microarrays disting gliomas. Cancer Res,2001,61(5): 1825-1829
[45]. M Bahn S C, Bac M S, Park Y B, Oh S I, Jeung J U, Bae J M, Chung Y S, Shin J S. Molecular cloning and characterization of a novel low temperature-induced gene, blti2, from barley (Hordeumvulgare L.) Biochim. Biophys Acta,2001,1522(2): 134-137
[46]Ballas N, Wong LM, Ke M, Theologis A. Two auxin-responsive domains interact positively to induce expression of the early indoleacetic acid-inducible gene PS-IAA4/5. Proc NatlAcadSci U S A. 1995, 92(8):3483-7
[47]Barre A, Peumans W J, Menu-Bouaouiche L, Els Van Damme J M, May G D, Herrera A F, Van Leuven F, Rouge P. Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit. Planta, 2000,211: 791-799.
[48]Becker D, Brettschneider R, Lorz H. Fertile transgenic Wheat from microprojectile bombardment of scutellar tissue. The plant Journal,1994,5(2):299-307
[49]Bertelli AAE, Giobannini L, Giannessi D, Migliori M, Bernini W, Fregoni M, Bertelli A. Antiplatelet activity of synthetic and natural resveratrol in red wine, Int J Tissue React 1995,17:1-3.
[50]Bonihi C, Pagni S, Vidrih R. Cell wall hydrolases and amylase in kiwifruir softening. Posrharvest Biology and Technology. 1996,9(1): 19-29
[51]Buzel R A, Cascardo J C, Rodrigues L A. Tissue-spcific regulation of BiP gene:a cis-acting regulatory domain is required for BiP promoter activity in plant meristems. Plant Mol. Biol. 2002,50(4-5):757-771.
[52] Chee M, Yang R, Hubbell E, Berno A, Huang XC, Stern D, Winkler J, Lockhart DJ, Morris MS, Fodor SP. Accessing genetic information with high-density DNA arrays. Science, 1996,274:610-614
[53] Chengappa S, Guilleroux M, Phillips W, Shields R. Transgenic tomato plants with decreased sucrose synthase are unaltered in starh and sugar accumulation in the fruit. Plant Mol. Biol. 1990,40:213-221
[54]Ching-Yi Wan, Thea A. Wilkins, 1994, A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton(Gossypium hirsutum L.) Analytical. Biochemistry 223,7-12
[55]Clendennen S K, Kipp P B, May G D. The role of ethylene in banana fruit ripening. In AK Kanellis, C Chang, H Kende, D Grierson, eds, Biology and Biotechnology of the Plant Hormone Ethylene. Kluwer Academic Publishers, Dordrecht, The Netherlands,1997a,115: 141-148.
[56]Clendennen S K, Lopez-Gomez R, Gomez-Lim M, Arntzen C J, May G D, The abundant 31-kilodalton banana pulp protein is homologous to classⅢ-acidic chitinase. Phytochenistry, 1998,47: 613-619.
[57]Clendennen S K, May G D. Differential gene expression in ripening banana fruit. Plant Physiol, 1997b,115:463-469.
[58]Clontech PCR-Select? cDNASubtraction Kit. 1996, CLONTECHniques\A-%
[59]Cordenunsi B R, Lajolo F M, Starch breakdown during banana ripening: sucrose synthase and sucrose phosphate synthase. J Agric Food Chem, 1995.43:347-351.
[60]Cordenunsi B R, Lajolo F M, Starch breakdown during banana ripening: sucrose synthase and sucrose phosphate synthase. J Agric Food Chem, 1995.43:347-351.
[61]CSIRO Division of Food Research. The ripening banana. ANANA standard colour index numbers for banana ripening. Melbourne:CSIRO 1985
[62]Deikman J, Xu RL, Kneissl ML, Ciardi JA, Kim KN, Pelah D. Separation of cis elements responsive to ethylene, fruit development, and ripening in the 5'-flanking region of the ripening-related E8 gene. Plant Molecular Biology 1998,37:1001-1011
[63] Diatchenko L, Lau Y F, Campbll A P, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD. Suppression subtractive hybridization:A method for generatiog differentially regulated or tissue-specific cDNA probes and libraries. Proc Notl Acad Sci, 1996,93:6025-6030
[64]Dolferus R, Jacobs M, Peacock WJ, Dennis ES. Differential interactions of promoter elements in stress responses of the Arabidopsis Adh gene. Plant Physiol. 1994 Aug;105(4):1075-87. [65]Dominguez-Puigjaner E, Immaculada L, Vendrell M, and Prat S. A cDNA clone highly expressed in ripe banana fruit shows homology to pectate lyases. Plant Physiol,1997,114:1071-1076
[66]Dominguez-Puigjaner E, Vendrell M, Ludevid D M. Differential protein accumulation in banana fruit during ripening. Plant Physiol, 1992,98:157-162.
[67]Dugdale B, Becker DK, Marding RM. Intron-mediated enchancement of the banana bunchy top virus DNA-6 promoter in banana(Musa spp.)embryogenic cells and planes. Plant Cell Reports.,,2001,,20:220-226
[68] Dugdale B,Beetham PR, Becker DK, Harding RM, Dale JL. Promoter activity associated with the intergenic regions of banana bunchy top virus DNA-1 to-6 in transgenic tobacco and banana cells. J Gen Virol, 1998,79:2301-2311
[69]Fodor SPA, Read J L, Huang X L. Multiples biochemical assaya with biological chips. Nature, 1993,364(6437):555-556
[70] Fodor SPA, Read J L, Pinung M, Stryer L, Lu AT, Solas D. Light- directed, spatially addressable parallel chemical synthesis. Science,1991,251(4995):767-773M
[71]Frankel EN, Waterhouse AL, Kinsella JE . Inhibition of human LDL oxidation by resveratrol. Lancet. 1993,24:1103-1104.
[72]Fukuda Y. Interaction of tobacco nuclear protein with an elicitor-responsive in the promoter of a basic class I chitinase gene. Plant Mol Biol 1997,34(1):81
[73]Gooding P S, Bird C, Robinson S P. Molecular cloning and characterization of banana fruit polyphenol oxidase. Plant, 2001,213:748-757
[74]Gubler and J. V. Jacobsen. Gibberellin-Responsive Elements in the Promoter of a Barley High-pl [alpha]-Amylase Gene . Plant Cell .1992,4: 1435-1441.
[75]Gurskaya N K, Diatchenko L, Chenckik A, Siebert PD, Khaspekov GL, Lukyanov KA, Vagner LL, Ermolaeva OD, Lukyanov SA, Sverdlov ED. Equalization cDNA subtraction based on selective suppression of ploymerase chain reaction; ckoning of Jurkat cell transcripts induced by phytohemaglutinin and phlrbol 12-myristate 13-ad. Analytical Biochemistry. 1996,240:90-97
[76] Hain R, Biesler B, Kindl H, Schroder G, Stocker R. Expression of a stilbene synthase gene in Nicotiana tabacum results in synthesis of phytoaiexin reveratrol. Plant Mol Biol. 1990,15:325-335.
[77]Hain R, Reif H-J, Krause E, Langebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E, Schreier PH, Stacker RH. Disease resistance results from foreign phytoaiexin expression in a novel plant. Nature .1993,361:153-156.
[78]Hermann SR, Harding RM, Dale JI. The banana actin 1 promoter drices near-constitutive transgene expression in vegetative tissues of banana(Musa spp.). Plant Cell Reports,2001,20:525-530
[79]Hill S, ap Rees T, The effect of glucose on the control of carbohydrate metabolism in ripening bananas. Planta. 1995b. 196:335-343.
[80]Hill S, ap Rees T, The effect of hypoxia on the control of carbohydrate metabolism in ripening bananas.Planta. 1995a. 197:313-323.
[81] Horsch RB, Fry JE, Hoffman N L, Eichholtz D, Rogers S G, Fraley R T. A simple and general method for transferring genes into plants. Science, 1985,227:1229-1231
[82]Huang P L, Do Y Y, Huang F C, Thay T S, Chang T W. Characterization and expression analysis of a banana gene encoding 1-aminocyclopropane-l-carboxylate oxidase. Biochem Mol Bio Int. 1997. 41:941-950.
[83]Ingram J, Bartels D. The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:377-403.
[84]Iwasaki T., Yamaguchi-Shinozaki K., and Shinozaki K. Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis. Mol. Gen. Genet. 1995,247,391-398.
[85]Iyare O A, Ekwukoma B O. Changes in the activities of carbohydrate-degrading enzymes with ripening in Musa paradisiaca. J. Sci Food Agric. 1992.58:173-176.
[86]Jiang M, Cai L, Udeani GO et al, Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science. 1997, 275-278.
[87]Jin Zhi-Qiang, ZHANG De-You, XU Bi-Yu. Cloning and Developmental and Tissue-Specific Expression of Banana Lectin Gene. Acta Genetica Sinica. 2004,31(5):508-512
[88]Jin Zhiqiang, Zhang Deyou, Xu Biyu. Using genomic DNA as standard for competitive RT-PCR to quantify BanLec gene expression in ripening banana. Research and development of tropical biological resources. Proceedings of the international workshop on research and development of tropical biological resources.2002,10. P.67-70.
[89]Jorda L, Vera P. Local and systemic induction of two defense-related subtilisin-like protease promoters in teansgenic Arabidopsis plant.Luciferin induction of PR fene expression. Plant Physiol.2000,124(3):1049-1058
[90]Josh G. DNA sequencers to go bananas? Science 2001 293: 585-586
[91]Kavaler E, Landman J, Chang Y, et al.. Detecting human bladder carcinoma cells in voided urine samples by assaying for the presence of telomerase activity. Cancer.1998,82(4);708-714
[92] Kim J Y, Chung Y S, Paek K H, Park Y I, Kim J K, Yu S N, Oh B J, Shin. Isolation and characterization of a cDNA encoding the cysteine proteinase inhibitor, induced upon flower maturation in carnction using suppression subtractive bybridization. Mol Cells, 1999,9(4):392-397
[93]Kimura Y, Okuda H, Arichi S . Effects of stilbenes on arachidonate metabolism in leukocytes. Biochim Biophys Acta . 1985,834:275-278.
[94]Kobayashi, S., Ding, CK, Nakamura, Y, Nakajima, I. and Matsumoto, R., Kiwifruit. (Actinida deliciosa) transformed with a Vitis stilbene synthase gene produce piceid. (resveratrol-glucoside). Plant Cell Rep. 2000,19: 904-910.
[95]Koch KE. Carbohydrate-modulated gene expression in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1996,41:509~540
[96]Kojima K, Sakurai N, Kuraishi S, Fruit softening in banana: correlation among stress-relaxation parameters, cell wall components and starch during ripening. Physiol Plant. 1994.90:772-778.
[97]Leckband G, Lorz H . Transformation and expression of a stilbene synthase gene of Vitis vinifera L in barley and wheat for increased fungal resistance. Theor Aappl Genet 1998,96:1004-1012
[98]Lee J M, William M E. Tingey S V, et al., DNA array profiling of fene expression changes during maize embryo development. Funct Integr Genomics. 2002,2:13-27
[99]Li-jia Qu. Jun Chen, Meihua Liu.et al., Molecular cloning and functional analysis of a NoveType of Bowman-Birk inhibitor gene family in Rice. Plant Physiology. 2003,133:560-570
[100]Liu X, Shiomi S, Nakatsuka A, Kubo Y, Nakamura R, Inaba A. Characterization of ethylene biosynthesis associated with ripening in banana fruit. Plant Physiol,1999121:1257-1265.
[101] Lockhart D J,Dong H, Byrne M C, Follettie MT, Gallo MV, Chee MS, Mittmann M, Wang C, Kobayashi M, Horton H, Brown EL. Expression monitoring by hybridization high-density oligonucleotide arrays. Nature Biote. 1996,14:1675-1680
[102]L6pez-G6mez R, Campbell A, Dong J G, Yang S F, Gomez-Lim M A. Ethylene biosynthesis in banana fruit: isolation of a genomic clone to ACC oxidase and expression studies. Plant Sci. 1997. 123: 123-131.
[103]Lutcke H A, Chow K C, Mickel F S. Selection of AUG in itiation codons differs in plants andanimals. The Embo. Journal, 1987,6:43-48
[104]Mariaa A C, Concepci N, Terry L. Differential regulation of small heat-shock gene in plants: analysis of a water-stress-inducible and developmentally activated sunflower promoter. Plant Molecular Biology. 1996,31:863-876
[105]Medina-Suarez R, Manning K, Fletcher J, Aked J Rird C R, Seymour G B, Gene expression in the pulp of ripening bananas. Plant Physiol, 1997.115:453-461.
[106]Mendoza E M T, Laurena A G, Uritani I, Astringency and polyphenols in banana. In I Uritani, VV Garcia, EMT Mendoza, eds, Posthavest Biochemistry of Plant Food-Materials in the Tropics. Japan Scientific Societies Press, Tokyo, 1994. p.177-191.
[107]Michel D, Salamini F, Bartels D, Dale P, Baga M, Szalay A. Analysis of a desiccation and ABA-responsive promoter isolated from the resurrection plant Craterostigma plantagineum. Plant J. 1993. 4(l):29-40.
[108]Nagao RT, Goekjian VH, Hong JC, Key JL. Identification of protein-binding DNA sequences in an auxin-regulated gene of soybean. Plant Mol Biol. 1993 21(6):1147-62.
[109] Nanjo T, Fujita M, Seki M, Kato T, Tabata S, Shinozaki K. Toxicity of free proline revealed in a Arabidopsis T-DNA-Tagged mutant deficient in proline dehydrogenase. Plant Cell Physiol.2003,44:541
[110]Nascimento J R O, Cordenunsi B R, Lajolo F M, Alcocer M J C, Banana sucrose-phosphate synthase gene expression during fruit ripening. Planta, 1997. 203:283-288.
[111] Negishi T, Nakanishi H, Yazakin J, Kishimoto N, Fujii F, Shimbo K, Yamamoto K, Sakata K, Sasaki T, Kikuchi S, Mori S, Nishizawa NK. cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests thar polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots. Plant J,2002,30(l):83-94
[112]Nicole Schupp, Paul Ziegler. The relation of starch phosphorylases to starch metabolism in wheat. Plant Cell physiol.2004,45(10):1471-1484
[113]Robinson N J, Procter C M, Connolly E L, Guerinot M L. A ferric-chelate reductase for iron uptake from soils. Nature. 1999,397:694-697
[114]Olive MR, Peacock WJ, Dennis ES. The anaerobic responsive element contains two GC-rich sequences essential for binding a nuclear protein and hypoxic activation of the maize Adhl promoter. Nucleic Acids Res. 1991. 19(25):7053-60.
[115] Pace-Asciak CR, Hahn S, Diamandis EP, Soleas G, Goldberg DM. The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: implications for protection against coronary heart desease. Clin Chim Acta 1995,235:207-219.
[116]Penarrubia L, Kim R, Giovannoni J. Production of the sweet protein monellin in transgenic plants. Bio/Technol. 1992,10:561-564
[117] Penarrubia L, Kim R, Giovannoni J, Kim S H, Fisher R. Production of the sweet protein monellin in transgenic plants. Bio/Technol. 1992,10:561-564
[118]Peter K. Busk and Montserrat Pages. Regulation of abscisic acid-induced transcription. Plant Molecular Biology. 1998,37(3):425-435
[119]Peumans W J, Zhang W, Barre A, Astoul C H, Balint-Kurti P J, Rovira P, Rouge P, May G D, Van Leuven F, Truffa-Bachi P, Van Damme E J M, Fruit-specific lectins from banana and plantain. Planta, 2000. 211:546-554.
[120]Pingyi Zhang, Roy L. Whistler, James N. BeMiller, Bruce R. Hamaker*. Banana starch: production, physicochemical properties,and digestibility—a review. Carbohydrate Polymers 59 (2005) 443-458
[121]Pua E C, Lee Y C, Expression of a ripening-related cytochrome P450 cDNA in Cavendish banana (Musa acuminata cv. Williams). Gene, 2003. 305:133-40
[122]Ralf P, Fritz S. Heat shock elements are involved in heat shock promoter activation during tobacco seed maturation. Plant Molecular Biology. 1996,31:157-162
[123]Reilly T P, Bourdi M, Brady JN, et al., Expression profiling of acetam inophen liver toxicity in mice using microarray technology. Biochem Biophys Res Common,2001,282(l):321-328
[124]Reymond P, Weber H, Damond M, et al., Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. Plant Cell,2000,12:707-719
[125]Robinson D J, Environmental risk assessment of release of transgenic plants containing virus-derived inserts. Transgen Res. 1996,5:359-362
[126]Ruan Y J, Gimore J, Onner T. Toward Arobidopsis genome analysis: monitoring expression profile of 1400 genes using cDNA microarrays. Plant J. 1998,15(6);821
[127]Samson JA., Tropical Fruits, London:longman, 1980,P119
[128]Schena M, Shalon D, Drvis R, et al., Quantitative monitoring of gene expression patterns with acomplementary DNA microarray. Science,1995,270(5235):467-470
[129]Schena M, Shalon D, Helle R,et al., Parallel human genome analysis: microarray-based expression monitorin of 1000 genes. Proc Natl Acad Sci USA.1996,93(20):10614-10619
[130]Schenk PM, Sagi LS. Remans T. Dietzgen RG. Bermard MJ. Graham MW. Manners JMA. Promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants. Plant Mol Biol, 1999,39:1221-1230
[131]Seki M, Narusaka M, Abe H. et al., Monitoring the expression pattern of 1300 Arabidopsis genes under draught and cold stresses by using a full-length cDNA microarray. Plant Cell.2001,13:61