版纳微型猪近交系骨髓间充质干细胞分化后的免疫原性和免疫调节作用
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摘要
背景
免疫抑制剂在器官移植和自身免疫性疾病的临床治疗中起着举足轻重的作用。但是，免疫抑制剂的副作用，包括免疫抑制引起的感染、肿瘤发生率增加等，也一直困扰着临床医生。在器官移植中，诱导受者对异基因移植物的特异性免疫耐受，减少、甚至撤除免疫抑制剂，一直是免疫学家和临床医生的一个梦想。这也是理论上解决异基因移植排斥，移植物抗宿主病等临床问题的最佳方案。
在器官或组织移植中，稳定嵌合与特异性耐受诱导密切相关。已有报道，骨髓间充质干细胞与造血干细胞联合移植在受者体内可检测到稳定的造血系嵌合，并能诱导对供者的特异性耐受。但骨髓移植前预处理的毒性、移植失败的风险和移植后出现的急、慢性移植物抗宿主病等问
Objective Mesenchymal stem cell (MSC) is a good choice for cell transplantation for its multilineage differentiation ability and low immunogenicity. Our previous studies in vitro indicated that undifferentiated MSC of swine are low immunogenic and could suppress proliferative response of human peripheral blood lymphocyte (hPBL) to different antigens. This study is aimed to learn the optimal temperature and serum for cultivation of Mesenchymal Stem Cells firstly, then to investigate whether differentiated MSC of swine can still keep their low immunogenicity and immune modulation ability in vitro for further study.
Methods MSCs were isolated from bone marrow of Banna Minipig Inbred-Line (BMI) and transfected with pSV3neo, a plasmid coding for SV40 large T antigen for its immortalization. Primary and immortalization
免疫抑制剂在器官移植和自身免疫性疾病的临床治疗中起着举足轻重的作用。但是，免疫抑制剂的副作用，包括免疫抑制引起的感染、肿瘤发生率增加等，也一直困扰着临床医生。在器官移植中，诱导受者对异基因移植物的特异性免疫耐受，减少、甚至撤除免疫抑制剂，一直是免疫学家和临床医生的一个梦想。这也是理论上解决异基因移植排斥，移植物抗宿主病等临床问题的最佳方案。
在器官或组织移植中，稳定嵌合与特异性耐受诱导密切相关。已有报道，骨髓间充质干细胞与造血干细胞联合移植在受者体内可检测到稳定的造血系嵌合，并能诱导对供者的特异性耐受。但骨髓移植前预处理的毒性、移植失败的风险和移植后出现的急、慢性移植物抗宿主病等问
Objective Mesenchymal stem cell (MSC) is a good choice for cell transplantation for its multilineage differentiation ability and low immunogenicity. Our previous studies in vitro indicated that undifferentiated MSC of swine are low immunogenic and could suppress proliferative response of human peripheral blood lymphocyte (hPBL) to different antigens. This study is aimed to learn the optimal temperature and serum for cultivation of Mesenchymal Stem Cells firstly, then to investigate whether differentiated MSC of swine can still keep their low immunogenicity and immune modulation ability in vitro for further study.
Methods MSCs were isolated from bone marrow of Banna Minipig Inbred-Line (BMI) and transfected with pSV3neo, a plasmid coding for SV40 large T antigen for its immortalization. Primary and immortalization
引文
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