本地红树林促生菌（溶P菌和固N菌）的分离及剂型研制
|
摘要
植物根际有益微生物菌剂研究及应用技术是倍受关注的国际性研究课题,其在根际定居(或定殖)是十分重要的,新剂型的开发有助于解决这一问题。随着根际微生物菌剂研究的不断深入,根际微生物对林木成活和生长的重要性愈来愈被世人所认识,应用研究普遍受到世界各国的重视。本论文以广东沿海地区本地红树林根际促生菌为研究对象,主要研究本地红树林根际促生菌,开展其分离筛选、培养基条件优化及胶囊剂型研制,使之成为一种存活率高、易于工业化生产和运输的新型菌剂。
本地红树林根际促生菌分离筛选。以广东南沙湿地公园采回的野生红树白骨壤小苗为材料,分离筛选出了具有溶P、固N能力的菌株,并以广东省林科院实验室从本地红树林根际分离筛选的溶P菌3株,编号为：P7#、07PCK、07pqwp1-5#；固N菌4种,编号为：NGQQ②D、NGWB③+④B、MLN2#、07Nhs-7#为供试菌株,在室温盆栽条件下研究了7种菌株对红树木榄幼苗(广东南沙湿地公园提供)的接种效应,结果表明：固N菌接种的木榄幼苗苗高比对照增加20.4%～31.8%,生物量比对照增加19.4%～21.6%,对木榄幼苗苗高及生物量进行显著性分析表明,苗高、生物量与对照相比均达极显著值；溶P菌接种的木榄幼苗苗高比对照增加22.8%～28.5%,生物量比对照增加19.4%～22.6%,对木榄幼苗苗高及生物量进行显著性分析表明,苗高、生物量与对照相比均达极显著值。在盆栽试验过程中,对促生菌的根部定殖情况进行了检测,溶P菌和固N菌根部定殖菌落数数量级均达109以上,结果表明,促生菌根部定殖良好。
根际促生菌培养基条件优化。鉴于不同环境下筛选出来的促生菌对培养基的适应性不一样,本研究以实验室自行筛选的促生菌为供试菌株,进行了单因素对促生菌生长影响研究,结果表明,促生菌最佳培养基条件为：盐度15‰,pH 8.0,营养源C源为葡萄糖,N源为蛋白胨。
本地红树林根际促生菌胶囊剂型研制。试验研制了本地红树林促生菌新剂型-微胶囊剂,选用壁材为海藻酸钠,采用锐孔-凝固浴法制做胶囊剂型。试验对微胶囊胶液配方进行了优化,通过对15种不同的胶液配方制作的微胶囊进行形态、硬度的比较,筛选出了最佳胶液配方：海藻酸钠(2.5%)：菌液混=3：1。试验将微胶囊剂型(干品、湿品)与液体剂型(4℃保存、-20℃保存)菌株的存活率进行比较,结果表明,常温下液体剂型对菌种的保存能力最差,2个月后存活率不足10万,供试溶P菌和固N菌活菌数仅为5.59x104、7.37×105,而胶囊剂干品常温保存2个月后,存活率仍能达到1000万,供试溶P菌和固N菌活菌数分别为11.37×106、10.13×107。在室温盆栽条件下研究了以本试验筛选出的促生菌制作的大胶囊、微胶囊和常规液体剂型(混合菌,单菌)对红树秋茄幼苗(广东南沙湿地公园提供)的接种效应,试验结果表明,接种促生菌菌剂的秋茄幼苗苗高比对照增加12.6%～23.0%,生物量比对照增加16.2%～17.5%。对秋茄幼苗苗高及生物量进行差异显著性分析表明,接种促生菌菌剂的秋茄苗高与对照相比均达极显著差异,生物量与对照相比,除NGQQ②D单菌液接种的秋茄幼苗外,均达极显著差异。各剂型促生菌根部定殖情况良好,尤以微胶囊效果最佳,而且胶囊剂干品携带、运输均比液体剂型方便,其优越性显而易见。
The research and applied technology of rhizosphere beneficial microbial inoculums is a repeatedly interested international research subject, which is very important to the field planting, new dosage forms can help to solve this problem. With study in a deep going way, the essentiality of rhizosphere microorganism to forest survival and growth was well-known by more and more people, applied research is generally well-focused by all countries in the world. In this paper, we considered rhizosphere growth-promoting bacteria of local mangrove in the coastal areas of Guangdong as object of study, focused on separating and selecting growth-promoting bacteria from local mangrove and developed a new bacterial agent of high survival rate and convenient in industrial production and transportation.
Isolation of growth-promoting bacteria from local mangrove. Used wild mangrove Avicennia marina seedlings that picked in Guangdong Nansha Wetland Park as experimental materials, we separated and selected bacterias which have the capacity of phosphate-solubilizing and nitrogen fixation, these seven kinds of growth-promoting bacteria,there are three phosphate-solubilizing bacterias selected by GuangDong Academy of Forestry, we number them P7#、07PCK、07pqwp1-5#; and also four kinds of Nitrogen-fixing bacteria, number them NGQQ②D、NGWB③+④B、MLN2#、07Nhs-7#. In pot experiments, we studied inoculation effect of seven kinds of strains on mangrove seedlings (offered by Guangdong NanSha wetland park) and the results showed comparing with contrast seedlings, the height of inoculated seedlings increased 20.4%～31.8% and the biomass increased 19.4%-21.6%, carried out significance analysis of the mass growth and biomass of mangrove seedlings, the results showed both of them reached extreme marked value; the height of phosphate-solubilizing bacteria inoculated seedlings increased 22.8%-28.5% and the biomass increased 19.4%-22.6%, carried out significance analysis of the mass growth and biomass of mangrove seedlings, the results showed both of them reached extreme marked value. In pot experiments, we tested the situation of field planting of rhizosphere growth-promoting bacteria, phosphate-Solubilizing Bacteria and Azotobacteria colonizing the Department of magnitude the number of each colony for more than 109, experimental results showed the situation of it was well. Optimization of culture medium of rhizosphere growth-promoting bacteria. Because wetland forest ecosystem is more complex than the dry land, and the growth conditions of growth-promoting were also relative complex, so optimize the standard medium of Phosphate-Solubilizing Bacteria. Via a series of single factor experiment, the best culture condition of Phosphate-Solubilizing Bacteria was:salinity 15%o, pH 8.0, carbon source dextrose, nitrogen source peptone.
Develop the capsule formulation of rhizosphere growth-promoting bacteria of local mangrove. In this paper, we optimized the recipes of microcapsule, compared the configuration and rigidity of microcapsule in different recipes and selected the best recipe:2.5% sodium alginate mixed with the bacteria liquid as 3:1. Compared the capacity of bacteria with conventional liquid dosage forms in different conditions, the preserving ability of liquid form in normal temperature was the worst, the survival rate was less than 10 million after two months, the viable counts of test Phosphate-Solubilizing Bacteria and Azotobacteria were only 5.59×104 and 7.37×105, but the survival rate of dry capsule can reach 10 million in normal temperature after preserving two months later; the viable counts of test Phosphate-Solubilizing Bacteria and Azotobacteria were 11.37×106 and 10.13×107 respectively. In pot experiments, we studied inoculation effect of large capsules, micro-capsules and liquid formulations of conventional (mixed bacteria, single bacteria) that are made by selected growth-promoting bacteria on mangrove kandelia candel seedlings (offered by Guangdong NanSha wetland park) and the results showed comparing with contrast seedlings, the height of inoculated seedlings increased 12.6%～23.0% and the biomass increased 16.2%～17.5%, carried out significance analysis of the mass growth and biomass of mangrove kandelia candel seedlings, the results showed the height of seedlings reached extreme marked value; the biomass of seedlings reached extreme marked value, excluded the seedlings that inoculated by single bacteria. We tested the situation of field planting of all kinds of dosages of rhizosphere growth-promoting bacteria, experimental results showed the situation of all dosages were well, especially the microcapsule. Also the dry capsules were convenient goods than conventional liquid formulations in carrying and storage, so the advantages of capsules are obvious.
本地红树林根际促生菌分离筛选。以广东南沙湿地公园采回的野生红树白骨壤小苗为材料,分离筛选出了具有溶P、固N能力的菌株,并以广东省林科院实验室从本地红树林根际分离筛选的溶P菌3株,编号为：P7#、07PCK、07pqwp1-5#；固N菌4种,编号为：NGQQ②D、NGWB③+④B、MLN2#、07Nhs-7#为供试菌株,在室温盆栽条件下研究了7种菌株对红树木榄幼苗(广东南沙湿地公园提供)的接种效应,结果表明：固N菌接种的木榄幼苗苗高比对照增加20.4%～31.8%,生物量比对照增加19.4%～21.6%,对木榄幼苗苗高及生物量进行显著性分析表明,苗高、生物量与对照相比均达极显著值；溶P菌接种的木榄幼苗苗高比对照增加22.8%～28.5%,生物量比对照增加19.4%～22.6%,对木榄幼苗苗高及生物量进行显著性分析表明,苗高、生物量与对照相比均达极显著值。在盆栽试验过程中,对促生菌的根部定殖情况进行了检测,溶P菌和固N菌根部定殖菌落数数量级均达109以上,结果表明,促生菌根部定殖良好。
根际促生菌培养基条件优化。鉴于不同环境下筛选出来的促生菌对培养基的适应性不一样,本研究以实验室自行筛选的促生菌为供试菌株,进行了单因素对促生菌生长影响研究,结果表明,促生菌最佳培养基条件为：盐度15‰,pH 8.0,营养源C源为葡萄糖,N源为蛋白胨。
本地红树林根际促生菌胶囊剂型研制。试验研制了本地红树林促生菌新剂型-微胶囊剂,选用壁材为海藻酸钠,采用锐孔-凝固浴法制做胶囊剂型。试验对微胶囊胶液配方进行了优化,通过对15种不同的胶液配方制作的微胶囊进行形态、硬度的比较,筛选出了最佳胶液配方：海藻酸钠(2.5%)：菌液混=3：1。试验将微胶囊剂型(干品、湿品)与液体剂型(4℃保存、-20℃保存)菌株的存活率进行比较,结果表明,常温下液体剂型对菌种的保存能力最差,2个月后存活率不足10万,供试溶P菌和固N菌活菌数仅为5.59x104、7.37×105,而胶囊剂干品常温保存2个月后,存活率仍能达到1000万,供试溶P菌和固N菌活菌数分别为11.37×106、10.13×107。在室温盆栽条件下研究了以本试验筛选出的促生菌制作的大胶囊、微胶囊和常规液体剂型(混合菌,单菌)对红树秋茄幼苗(广东南沙湿地公园提供)的接种效应,试验结果表明,接种促生菌菌剂的秋茄幼苗苗高比对照增加12.6%～23.0%,生物量比对照增加16.2%～17.5%。对秋茄幼苗苗高及生物量进行差异显著性分析表明,接种促生菌菌剂的秋茄苗高与对照相比均达极显著差异,生物量与对照相比,除NGQQ②D单菌液接种的秋茄幼苗外,均达极显著差异。各剂型促生菌根部定殖情况良好,尤以微胶囊效果最佳,而且胶囊剂干品携带、运输均比液体剂型方便,其优越性显而易见。
The research and applied technology of rhizosphere beneficial microbial inoculums is a repeatedly interested international research subject, which is very important to the field planting, new dosage forms can help to solve this problem. With study in a deep going way, the essentiality of rhizosphere microorganism to forest survival and growth was well-known by more and more people, applied research is generally well-focused by all countries in the world. In this paper, we considered rhizosphere growth-promoting bacteria of local mangrove in the coastal areas of Guangdong as object of study, focused on separating and selecting growth-promoting bacteria from local mangrove and developed a new bacterial agent of high survival rate and convenient in industrial production and transportation.
Isolation of growth-promoting bacteria from local mangrove. Used wild mangrove Avicennia marina seedlings that picked in Guangdong Nansha Wetland Park as experimental materials, we separated and selected bacterias which have the capacity of phosphate-solubilizing and nitrogen fixation, these seven kinds of growth-promoting bacteria,there are three phosphate-solubilizing bacterias selected by GuangDong Academy of Forestry, we number them P7#、07PCK、07pqwp1-5#; and also four kinds of Nitrogen-fixing bacteria, number them NGQQ②D、NGWB③+④B、MLN2#、07Nhs-7#. In pot experiments, we studied inoculation effect of seven kinds of strains on mangrove seedlings (offered by Guangdong NanSha wetland park) and the results showed comparing with contrast seedlings, the height of inoculated seedlings increased 20.4%～31.8% and the biomass increased 19.4%-21.6%, carried out significance analysis of the mass growth and biomass of mangrove seedlings, the results showed both of them reached extreme marked value; the height of phosphate-solubilizing bacteria inoculated seedlings increased 22.8%-28.5% and the biomass increased 19.4%-22.6%, carried out significance analysis of the mass growth and biomass of mangrove seedlings, the results showed both of them reached extreme marked value. In pot experiments, we tested the situation of field planting of rhizosphere growth-promoting bacteria, phosphate-Solubilizing Bacteria and Azotobacteria colonizing the Department of magnitude the number of each colony for more than 109, experimental results showed the situation of it was well. Optimization of culture medium of rhizosphere growth-promoting bacteria. Because wetland forest ecosystem is more complex than the dry land, and the growth conditions of growth-promoting were also relative complex, so optimize the standard medium of Phosphate-Solubilizing Bacteria. Via a series of single factor experiment, the best culture condition of Phosphate-Solubilizing Bacteria was:salinity 15%o, pH 8.0, carbon source dextrose, nitrogen source peptone.
Develop the capsule formulation of rhizosphere growth-promoting bacteria of local mangrove. In this paper, we optimized the recipes of microcapsule, compared the configuration and rigidity of microcapsule in different recipes and selected the best recipe:2.5% sodium alginate mixed with the bacteria liquid as 3:1. Compared the capacity of bacteria with conventional liquid dosage forms in different conditions, the preserving ability of liquid form in normal temperature was the worst, the survival rate was less than 10 million after two months, the viable counts of test Phosphate-Solubilizing Bacteria and Azotobacteria were only 5.59×104 and 7.37×105, but the survival rate of dry capsule can reach 10 million in normal temperature after preserving two months later; the viable counts of test Phosphate-Solubilizing Bacteria and Azotobacteria were 11.37×106 and 10.13×107 respectively. In pot experiments, we studied inoculation effect of large capsules, micro-capsules and liquid formulations of conventional (mixed bacteria, single bacteria) that are made by selected growth-promoting bacteria on mangrove kandelia candel seedlings (offered by Guangdong NanSha wetland park) and the results showed comparing with contrast seedlings, the height of inoculated seedlings increased 12.6%～23.0% and the biomass increased 16.2%～17.5%, carried out significance analysis of the mass growth and biomass of mangrove kandelia candel seedlings, the results showed the height of seedlings reached extreme marked value; the biomass of seedlings reached extreme marked value, excluded the seedlings that inoculated by single bacteria. We tested the situation of field planting of all kinds of dosages of rhizosphere growth-promoting bacteria, experimental results showed the situation of all dosages were well, especially the microcapsule. Also the dry capsules were convenient goods than conventional liquid formulations in carrying and storage, so the advantages of capsules are obvious.
引文
[1]林鹏.中国红树林生态系[M].北京：科学出版社,1997(中文版),1999(英文版).
[2]林鹏.中国红树林研究进展[J].厦门大学学报,2001,Q938：592-603.
[3]王幼珊,张美庆等.VA菌根真菌抗盐碱菌株筛选[J].土壤学报,1994(31增)：79-83.
[4]陈华癸.土壤微生物学[M].上海：上海科学技术出版社,1981.
[5]Lynch JM,Whipps JM.Substrate flow in the rhizosphere[J].Plant Soil,1990, 129:1-10.
[6]Hartmann A,Schmid M,Wenzel W,Hinsinger Ph.Rhizosphere 2004-Perspectives and Challenges-ATribute to Lorenz Hiltner[J].Munich,Germany:GSF-National Research Center for Environment and Health,2005.
[7]Kowalchuk GA,Buma DS,de Boer W, Klinkhamer PGL, van Veen JA. Effects of above-ground plant species composition and diversity on the diversity of soil-borne microorganisms[M]. Anton.Leeuw.Int.J.G.,2002,81:509-520.
[8]Zhang FS,Shen JB,Li L,Liu XJ.An overview of rhizosphere processes related with plant nutrition in majorcropping systems in China[J].Plant Soil,2004,260: 89-99.
[9]Johansson JF,Paul LR,Finlay RD.Microbial interactions in the mycorrhizosphere and their significance for sustainable agriculture[J].FEMS Microbiol.Ecol.,2004, 48(1):1-13.
[10]Preston GM. Plant perceptions of plant growth-promotingpseudomonas. Philosophic. Trans.Royal Soc.London Seri[J]. Biol.Sci.,2004,359(1446):907-918.
[11]Ramette A,Frapolli M,Defago G, Moenne-Loccoz Y.Phylogeny of HCN ynthase-encoding hcnBC genes in biocontrol fluorescent pseudomonads and its relationship with host plant species and HCN synthesis ability[M]. Molecul.Plant-Micro.Interact.,2003,16(6):525-535.
[12]Kksepper,Leong T,Tdntze M,et al.l980.Enhanced plant growth siderphones produced by plant growth-promoting rhizobacteria[J].Nature,286:885-886.
[13]Lalitha B.1989.Phytotoxin produced by Diaporthe phaseolorumvar.caulivora,the causal organism of stern canker of soybean [J].Phytopathology,79:499-504.
[14]Long W.W(龙伟文),Wang P(王平),Feng X.M(冯新梅)2000.Research progress on PGPR AMF interactions[J].AppZ Ecol(应用生态学报),11(2):311-314(in Chinese).
[15]PenroseDM an dGlickBR.2003.Methodsforisolatingand charac.terizing ACC deaminase-containing plant growth-promoting rhizobacteria[J].Phsiol Plantarum, 118:10-15.
[16]RamamoorthyV,Visw'anathan R,Raguchander T,et al.2001.Induction of systemic resistance by plantgrowth promoting rhizobac.teriain crop plains against pestsand diseases[J].CtopProuc,20:1-11.
[17]Ramamoorthy V,Raguchan der T and Samiyappan R.2002.En.hancing resistance of tomato and hot peppertoPythium diseases by seed treatment with Fluorescent pseudomonads[J].Eut'o J knt PathoZ.108:429-441.
[18]Rangeshwaran R,W asnikar AR,Prased PD,et al.2002.Isolation of endophyfic bacteria for biological control of wilt pathogens[J].BiolControl.16:125-134.
[19]Ryu CM,Farag MA,Hu CH,et al.2003.Bacterial volatiles promote growth in Arabidopsis[J]. Proc Natl Acad Sci USA,100:4927-4932.
[20]Silva H and Sandro A.2003.Development of a root colonization bioassay for rapid screening of rhizobacteria for potential bioeontrol agents[J]. Phytopathol.151:42-46.
[21]Sc hippers B,Bakker AW,Ba ckker PA.1987.Interactions of delete, rious and beneficial rhizosphere microoganisms[J].Annu Rev Phytopathol,25:339-358.
[22]Snders, RE., Tinker, P.B.1973. phosrhato flow into my-corrhizal root. Pestic.Sci[J].1973(4):385-395.
[23]Paxfitt, R.L..1979, Plant and soil[M].53:55-56.
[24]刘润进,李晓林.丛枝菌根及其应用[M],科学出版社,2000.
[25]RICE RONALD W.DATNOFF LAWRENCE E. RAID RICHARDN,et al. Influence of vesicular-arbuscular mycorrhizae on celery transplant growth and phosphorus use efficiency[J].Journal of Plant Nutrition,2002,25(8):1839-1853.
[26]吴铁,覃照宇等.VA菌根及其对土壤中磷的作用[J].湖南农业科学,2005,(2)：41-43.
[27]赵俊兴,郭柏寿等.VA菌根与植物的磷素营养[J].山东农业大学学报,2001.32(3)：401-405.
[28]李晓林,姚青.VA菌根与植物的矿质营养[J].自然科学进展,2000(6)：524-531.
[29]曾曙才,苏志尧等.VA菌根真菌对植物养分吸收与传递的影响[J].西南林学院学报.2005(1)：72-74.
[30]郑伟文,宋亚娜.VA菌根真菌和根瘤菌对翼豆生长、固氮的影响[J].福建农业学报,2000,15(2)：50-55.
[31]Barea Jm,Anderadeg,Binciotidv,et al.Arbuscular mycorrhizas in sustainable soil plant systems[A].BHOCK,AVARMA.Mycorrhiza stureutre,function,molecular biology and biotechnology[C].Springer-Verlag,Heidelberg,Ger-many,1995.521-559.
[32]Tarafdar J c.Effect of vesicular-arbuscular mycorrhizal and phosphatase-producing fungal inoculation on growth and nutrition of white clover supplied with organic phosphorus[J].Folia Microbiologica,1995,40(3):327-332.
[33]易时来,温明霞.VA茵根改善植物磷素营养的研究进展[J].中国农学通报,2004(5)：164-166.
[34]林先贵,郝文英等.VA菌根对植物耐旱、涝能力的影响[J].土壤,1992.24(3)：142-145.
[35]Subramanian,K. S. and Charest, C. Arbuscular mycorrhizae and nitrogen assimilation in maize after drought and recovery [J]. Physiologiu Plantarum,1998, 102(2):285-296.
[36]赵士杰,李树林.VA菌根促进韭菜增产的生理基础研究[J].土壤肥料,1993(4)：38-40.
[37]王幼珊,张美庆等.VA菌根真菌抗盐碱菌株筛选[J].土壤学报,1994(31增)：79-83.
[38]Feng Gu et al Effect of arbuscular mycorrhizal fungus on the growth of under Nacl stress condition[A],In:Ula.A J.et al (eds).2nd lctl Conf on Mycro[C].Uppsala, Sweden.1998.61.
[39]熊礼明,史瑞和.蒸汽灭菌的土壤对植物的毒害作用及VA菌根的减毒效应[J].土壤学报,1994(31增)：234-239.
[40]Marx D H,Ohio J.Science.[C],1975(75):288-297.
[41]弓明钦,陈应龙,仲祟禄.茵根研究及应用[M].北京：中国林业出版社,1997.
[42]王曙光,林先贵等.丛枝菌根与植物的抗逆性.生态学杂志[J].2001,20(3)：27-30.
[43]KLOEPPER J W,LIFSH1TA R,ZABLOTOWICA R M.Free-living Bacterial Inoculation for Enhancing Crop Productivity[J].Trends in Biotechn,1989,7: 39-43.
[44]BACKER J0,COOKR J.Role of Sidemphoresin Suppression of Pythium Speciesand Production of Increased-growth Response of Wheat by Fluoescent Pseudomonads[J].Phytopathology,1988,78:778-782.
[45]OOSTENDORP M,SIKORA R A.Seed Treatment with Antagonisitic Rhizobacteria for the Suppression of Heterodera Schachtii Early Root Infection of Sugar Beet[J].Revue de Nematologie,1989,12(1):77-83.
[46]RACKE J,SIKORA R A.Isolation,Formulation and Antagonlstc Activity of Rhizobacteria Toward the Potato Cyst Nematode Globodera Pallida[J].Soil Biology and Biochemistry,1992,24(6):521-526.
[47]CHANWAY C P.Endophytes:They're not Just Fungi[J].Can.J.Bot.,1996,74: 321-322.
[48]葛诚主编.微生物肥料的生产应用及其发展,中国农业科技出版社,1996.
[49]吴建峰,林先贵.土壤微生物在促进植物生长方面的作用.土壤.2003,35(1)：18-21.
[50]BROADBENTP,et al.Bacteria andActinomycetesAntagonistic to Fungal Root Pathogensin AustralianSoil[J].Aust J Biol Sci,1971,24:925-944.
[51]WELLER D M.Biological Control of Soil Borne Pathogens in the Rhizosphere with Bacteria[J].Annu Rev phytopathol,1988,(26):379-407.
[52]王金生,赵立平,冯家勋.重组植物病害防治微生物[c]//黄大,林敏.农业微生物基因工程.北京：科学出版社,2001,316-408.
[53]Brockwell J, Bottomley P J.Soil Biol and Biochem,1995,27:683-697.
[54]Rodriguez-Navarro D N, Temprano F, Orvive R.Soil Biol and Biochem[C], 1991,23:375-379.
[55]刘惠琴,宁国赞,管南珠.土壤通报,1994,24(3)：144-145.
[56]Temprano F J, Albareda M, Camacho M,et al. Int Microbiol[M],2002,5:81-86.
[57]周本新,凌世海,尚鹤言等.农药新剂型.化学工业出版社,1994,405-420.
[58]徐莉,杨红,彭建新等.荧光增白剂对苏云金杆菌毒力的增效作用及其紫外防护功效的研究.中国病毒学.2000,15：244.
[59]A.Lamprecht,U.F.Schaefer,C.-M.Lehr.Characterization of microcapsules by confocal laser scanning microscopy:structure, capsulewall composition and encapsulation rate[J]. European Journal of Pharmaceutics and Biopharmaceutics. 2000,49:1-9.
[60]梁治齐.微胶囊化技术及应用[M].北京：中国轻工业出版社.1999.
[61]李玉新,敖聪聪.微胶囊技术及农药微胶囊剂[J].农药.1998,3(7)：4-7.
[62]Patrick B,OD onnell,JamesW.McGinity.Preparation of microspheres by the solvent evaporation technique[J].Advanced Drug DeliveryReviews.1997,28: 25-42.
[63]Anna Shulkin, Harald D.H.SP ver.Polymer microcapsules by interfacial poly addition between styrene-maleic anhydride copolymers and amines[J].Journal of Membrane Science.2002,209:421-432.
[64]Y.F.Fan, X.X.Zhang, X.C.Wang, J.L,i Q.B.Zhu.Super cooling prevention of microencapsulated phase change material[J]. There much in ica A cta.2004, 413:1-6.
[65]袁青梅,杨红卫,杨恺等.微胶囊农药新剂型[J].云南大学学报(自然科学版).2002,24(1 A)：95-97.
[66]史宁,崔光华.流化床包衣制备微囊技术的发展及应用[J].国外医学药学分册.200229(6)：336-340.
[67]K.Dewettinck, A.HuyghebaertFluidized bed coating in food Technology[J].Trends in Food Science& Technology.1999,10:163-168.
[68]栾庆书.外生菌根真菌应用技术研究现状[J].辽宁林业科技.2002(3,4)：67-69.
[69]张亚莲.微生物肥料的功效及在茶树上的利用[J].茶叶通讯.2003,(1)：19-22.
[70]田永辉.不同茶园群落茶树根际微生物群落生态特征[J].茶叶通讯.2001(#)：24-28.
[71]刘柏玉,雷泽周.VA菌根对茶苗生长及养分吸收的影响[J].中国茶叶.1995(5)：6-7.
[72]张亚莲.微生物肥料的功效及在茶树上的利用[J].茶叶通讯.2003,(1)：19-22.
[73]林松,王香甩,宋维希.光合菌肥在茶树上的应用[J].福建茶叶.2002,(2)：22.
[74]叶水娟.茶园施用酵素菌肥料初步试验.中国茶叶[J].2004,26(1)：40-41
[75]许允文、韩文炎、石元值等.茶园施用多效有机菌肥的试验效果[J].中国茶叶.2001,23(4)：12-14.
[76]郭秀珍等编著,1989.内生菌根、林木菌根及应用技术[M].中国林业出版社,80-131.
[77]万水林,1992.柑桔菌根容器育苗技术研究[M].江西农业科技,(6)：26-28.
[78]林鹏.1997.中国红树林生态系[M].北京：科学出版社,23-30.
[79]Jones EBG, Hyde KD.1988. Methods for the study of marine fungi from the mangroves [A]. In:Agate AD, eds. Mangrove Microbiology:Role of Microorganism in Nutrient Cycling of Mangrove Soils and Waters [C]. Paris UNDP/UNESCO,9-27.
[80]Zhuang T,Cheng L P.1998. Soil microbial function of Kandelia candel mangrove: degradation of diesel oil [A]. In:Morton B,ed. The Marine Biology of the South China Sea[C]. Hongkong:Hongkong University Press,389-395.
[81]Alongi DM.1988.Bacterial productivity and microbial biomass in tropical mangrove sediments[J]. Microb. Ecol.,15:59-79.
[82]曾会才‘,郑服丛,贺春萍.2001.海南红树林生境中海疫霉种的分离与鉴定[J]菌物系统,20(3)：310-315.
[83]Zuberer DA,Silver WS.1978. Biological dinitrogen fixation (Acetylene reduction) associated with Florida mangroves[J].Appl.Environ. Microbiol.,35:567-575.
[84]Zuberer DA,Silver WS.1979. N2-fixation (acetylene reduction)and the microbial colonization of mangrove roots[J]. New Phytol.,82:467-471.
[85]Sengupta A,Chaudhuri S.1990. Halotolerant Rhizobium strains from mangrove swamps of the Ganges River Delta[J]. Indian J.Microbiol.,30:483-484.
[86]Holguin QBashan Y,Mendoza2Salgado RA, et al.1999. Microbiology of mangroves,forests in the frontier between land and sea[J].Ciencia Desarrollo,25 (144):26-35.
[87]郑志成,周美英,姚炳新.1989.红树林根系放线菌的组成[J].厦门大学学报(自然科学版),28(3)：306-310.
[88]Bashan Y,Puente ME,Myrold DD, et al.1998. In vitro transfer of fixed nitrogen from diazotrophic filamentous cyanobacteria to black mangrove seedlings [J] FEMS Microbiol. Ecol.,26 (3):165-170.
[89]Ellison AM, Farnsworth EJ, Twilley RR.1996. Facultative mutualism between red mangrove and root2fouling sponges in Belizean mangal[J]. Ecology,77 (8):2431-2444.
[90]Toledo G,Bashan Y,Soeldner A.1995. In vitro colonization and increase in nitrogen fixation of seedling roots of black mangrove inoculated by a filamentous cyanobacteria[J]. Can. Microbiol.,41 (11):1012-1020.
[91]van der Valk AG,Attiwill PM.1984. Acetylene reduction in an A vicennia mari na community in southern Australia [J]. A ust J. Bot.,32:157-164.
[92]Zuberer DA,Silver WS.1978. Biological dinitrogen fixation (Acetylene reduction) associated with Florida mangroves[J].Appl.Environ. Microbiol.,35:567-575.
[93]Vazquez P,Holguin G,Puente ME, et al.2000. Phosphate2solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon [J]. Biol. Fertil. Soils,30:460-468.
[94]Saxena D,Loka2Bharathi PA,Chandramohan D.1988. Sulfate reducing bacteria from mangrove swamps of Goa,central west coast of India[J]. Indian J. Mar. Sci.,17:153-157.
[95]Vethanayagam RR.1991. Purple photosynthetic bacteria from a tropical mangrove environment [J]. Mar. Biol.,110:161-163.
[96]Vethanayagam RR, Krishnamurthy K.1995. Studies on anoxygenic photosynthetic bacterium Rhodopseudomonas sp. from the tropical mangrove environment [J]. Indian J. Mar. Sci.,24:19-23.
[97]Chandrika V,Nair PVR, Khambhadkar LR.1990. Distribution of phototrophic thionic bacteria in the anaerobic and microaerophilic strata of mangrove ecosystem of Cochin [J]. Mar.Biol. Assoc. India,32:77-84.
[98]Dhevendaran K.1984. Photosynthetic bacteria in the marine environment at Porto2Novo [J]. Fish Technol. Soc. Cochi n.,21:126-130.
[99]Shoreit AAM,E12Kady IA,Sayed WF.1994. Isolation and identification of purple nonsulfur bacteria of mangal and non2mangal vegetation of Red Sea Coast,Egypt [J]. Limnologica,24:177-183.
[100]Gotto JW,Taylor BF.1976. N2 fixation associated with decaying leaves of the red mangrove (Rhizophora mangle) [J]. A ppl.Envi ron. Microbiol.,31 781-783.
[101]Mohanraju R,Rajagopal BS,Daniels L, et al.1997. Isolation and characterization of a methanogenic bacteriumfrom mangrove sediments[J]. Mar. Biotechnol.,5:147-152.
[102]Raghukumar S, Sathepatak V, Sharma S, et al.1995. Thraustochytrid and fungal component of marine detritus.3. Field studies on decomposition of lesves of the mangrove Rhizopphora apiculata[J]. A quat. Microb. Ecol.,9 (2):117-125.
[103]Hyde KD,Lee SY.1995. Ecology of mangrove fungi and their role in nutrient cycling:What gaps occur in our knowledge[J]. Hydrobiologia,295 (1-3) 107-188.
[104]Haronian D,Lewis A.1991. Element of a unique bacteriorhodopsin neural network architecture[J]. Appl. Optics,30(5):597-608.
[105]Miyasaka T, Koyama K, Itoh I.1992. Science quantum conversion and image detection by a bacteriorhodopsin2based artificial photoreceptor[J].Science,255 (1):342-344.
[106]张永光,李文均,姜成林,等.2002.嗜盐放线菌的研究进展[J].微生物学杂志,22(4)：45-48.
[107]Maltseva.O,Oriel.P..1997.Monitoring of an alkaline 2,4, trichloropheno degrading enrichment culture by DNA fingerprinting methods and isolation of the responsible organism,Haloakaliphilic Nocardioi des sp. strain M6 [J].Appl. Environ. Microbiol.,63:4145-4149.
[108]刘爱民.2002.嗜盐菌的研究进展[J].安徽师范大学学报(自然科学版),25(2)：181-193.
[109]卢青.2000.植物耐盐性的分子生物学研究进展[J].生物学杂志,17(4)9-11.
[110]林鹏.1990.红树林研究论文集(第1集)[C].厦门：厦门大学出版社,23-30.
[111]林鹏,陈荣华.1991.红树林有机碎屑在河口生态系统中的作用[J].生态学杂志,10(2)：45-48.
[112]云南大学生物系.1980.植物生态学[M].北京：人民教育出版社,310-311.
[113]卢昌义,林鹏.1989.两种红树植物叶分解速率的研究[J].厦门大学学报(自然科学版),27(6)：679-683.
[114]Lu CY,Lin P.1990. Studies on liter fall and decomposition of B ruguier sexangula (Lour) poir community on Hainan Island,China[J]. B ull. Mar. Sci.,47 (1):139-148.
[115]Alongi DM.1994. The role of bacteria in nutrient recycling in tropical mangrove and other coastal benthic ecosystems[J]. Hydrobiologia,285:19-32.
[116]Alongi DM,Christoffersen P, Tirendi F.1993. The influence of forest type on microbial2nutrient relationships in tropical mangrove sediments[J]. Ex p. Mar. Biol.Ecol.171:201-223.
[117]庄铁城,林鹏.1992.九龙江口秋茄红树林凋落叶自然分解与落叶腐解微生物的关系[J].植物生态学与地植物学学报,16(1)：17-25.
[118]Kohlmeyer J,Bebout B,Volkmann KB.1995. Decomposition of mangrove wood by marine fungi and teredinids in Belize [J].Mar. Ecol.,16:27-39.
[119]Raghukumar S,Sharma S,Raghukuma C, et al.1994. Thraustochytrid and fungal component of marine detritus.4. Laboratory studies on decomposition of leaves of the mangrove Rhizophora apiculata Blume[J].Ex p. Mar. Biol Ecol.,183 (1):113-131.
[120]Wu RY.1993. Studies on the microbial ecology of Tansui Estuary [J]. Bot. B ull. Acad. Sin.,34(1):13-30.
[121]Bano N,Nisa MU, Khan N, et al.1997. Significance of bacteria in the flux of organic matter in the tidal creeks of the mangrove ecosystem of the Indus river delta, Pakistan [J]. Mar. Ecol.Prog. Ser.,157:1-12.
[122]Alongi DM,Boto KG, Tirendi F.1989. Effect of exported mangrove litter on bacterial productivity and dissolved organic carbon fluxes in adjacent tropical nearshore sediments [J]. Mar. Ecol.Prog. Ser.,56:133-144.
[123]Boto KG,Alongi DM,Nott ALJ.1989. Dissolved organic carbonbacteria interactions at sediment2water interface in a tropical mangrove system[J]. Mar. Ecol.Prog. Ser.,51:243-251.
[124]Stanley SO,Boto KQAlongi DM, et al.1987. Composition and bacterial utilization of free amino acids in tropical mangrove sediments[J]. Mar. Chem,22:13-30.
[125]Day JW J r eds.1989. Microbial ecology and organic detritus in estuaries[A]. In: Day JW J r,eds. Estuarine Ecology [C]. New York John Wiley & Sons, Inc.,257-308.
[126]Gina H,Patricia V,Yoav B.2001. The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems:An overview[J].Biol.Fert.Soils,33(4):265-278.
[127]Rivera2Monroy VH,Twilley RR.1996. The relative role of denitrification and immobilization in the fate of inorganic nitrogen in mangrove sediments (Terminos Lagoon,Mexico) [J]. Limnol.Oceanogr.,41:284-296.
[128]Rivera2Monroy VH,Day WJ, Twilley RR, et al.1995a. Flux of nitrogen and sediment in a fringe mangrove forest in Terminos lagoon,Mexico[J]. Est uar. Coast. S helf Sci.,40:139-160.
[129]Rivera2Monroy VH,Twilley RR,Boustany RG, et al.1995b. Direct denitrification in mangrove sediments in Terminos Lagoon,Mexico[J]. Mar. Ecol.Prog. Ser.,126:97-109.
[130]Tiedje JM.1988. Ecology of denitrification and dissimilatory nitrate reduction to ammonium[A]. In:Zehnder AJB,ed. Biology of Anaerobic Microorganisms [C]. New York:Wiley,179-244.
[131]Jetten MSM,Strous M,van de Pas2Schoonen KT, et al.1998.The anaerobic oxidation of ammonium [J]. FEMS Microbiol.Rev.,22:421-437.
[132]Bano N,Nisa MU, Khan N, et al.1997. Significance of bacteria in the flux of organic matter in the tidal creeks of the mangrove ecosystem of the Indus river delta, Pakistan [J]. Mar. Ecol.Prog. Ser.,157:1-12.
[133]Robertson AI,Daniel PA.1989. Decomposition and the annual flux of detritus from fallen timber in tropical mangrove forests[J]. L imnol. Oceanogr.,34 640-646.
[134]D'Croz L,Del Rosario J, Holness R.1989. Degradation of red mangrove (Rhizophora mangle) leaves in the bay of Panama[J]. Rev. Biol. Trop. 37:101-104.
[135]Odum WE, Heald EJ.1975. Mangrove forests and aquatic productivity[A]. In: Hasler AD,ed. Coupling of Land and Water Systems:Ecological Study No.10 [C]. New York:SpringerVerlag,129-136.
[136]Sengupta A,Chaudhuri S.Ecology of hetemtrophic dinitrogen fixation in the rhlzosphere of rllangrove plant community at the Ganges fiver estuaryinIndia [J].Oeoologia,1991,87:560-564.
[137]Holguin G, Guzman M A,Bashan Y.Two new nitrogen-fixing Bacteria from the rhizosphere of mangrove trees:their isolation,identification and in vitro interaction with rhizosphere Staphylococcussp[J].FEMS Microhiol Ecol,1992, 101:207-216.
[138]Abllgi D M,Christoffersen P,Tirendi F.The influenoe of forest type on microbial-nutrient relationships in tropical m grove sedlmerits[J].Exp Mar Biol Ecol,1993,171:201-223.
[139]Vazquez P,Holguin G,PuenteM E,et al.Phosphate-solubilizing microorganisms associated with the rhizosphere of rllangroves in a semiarid coastal,Bgoon[J].Biol Fertil Soils,2000,30:460-468.
[140]Holguin G,Bashan Y,Mendoza-Salgado R A,et al.La Micmbiologia de los manglares.Bosques en la frontem entre el mar Y la tierrra[J].Cicnciay Desarrollo,1999,1ZM:26-35.
[141]Holguin G, Vazquez P,Bashan Y.The role of sediment microorganisms in the productivity,conservation,and rehabilitation of the mangrove ecosystems:nil overview[J].Biol Fertil Soils,2001,33:265-278.
[142]董俊德,王汉奎,张偬,等.海洋固氯生物多样性及其对海洋生产力的氮、碳贡献[J].生态学报,2002,22(10)：1741-1749.
[143]Van der Valk A G,Attiwill P M.Acetylene reduction in Itll Avcenna marna community in Southem Australia[J].Aust J Bot,1984.32:157-164.
[144]Sengupta A,Chaudhufi S.Ecology of hetemtrophic dinitrogen firmtion in the rhizosphere of mangrove plant community at the Gangesriver estuary in India[J].Oecologia,1991,87:560-564.
[145]Vasqucz P,Holguin G,Pucntc M E,et al.Phosphate-solubilizingmicroorganisms associated with the rhizosphere of mangroves in flsemiarid coastal lagoon[J].Biol Fcail Soils,2000,30:460-468.
[146]Bashan Y,Hoguin G.Azospirillum-plant relationships:environmental and physiological advances(1990-1996)[J].Can J Micmbiol,1997,43:103-121.
[147]TANDON H L S.Fertilizers,Organic Manures,Recyclable Wastes and Bio-fertilizers[M].New Delhi(India):Fertilizer Development and Consulation Organisation,1992.
[148]陈廷伟,葛诚.我国微生物肥料发展趋向[J].土壤肥料,1995,(6)：16-20.
[149]葛诚主编.微生物肥料的生产应用及其发展[M].北京：中国农业科技出版社,1996.
[150]Puente M E,aols.G,e ck BR,et al.Root surface colonization ofblack nlBflgFove seedlings byAzz-pirilhm halopmeferens and Azospirillum brasilensein seawater [J].FEMS Microbiol Ecol,1999,29:283-292.
[151]Toledo G,BashanY,s0|ner A.In vitrocolonization andincrease in nitrogen fix ation of Ilg roots of black nlangrove inoculated by a filamentous cyanobacteria[J].Can J Mierobiol,1995,41:1012-1020.
[152]Bashan Y,Puente M E,Myrold D D,et al.In vitro transf of fixed nitrogen from diazotrophic fdamentous cyanobacteria to black man grove seedlings[J].FEMS Microbiol Ecol,1998,26:165-170.
[153]Bashan Y,Hols.G.Azospirillum-plant relationships environmental and physi gical advances(1990-1996) [M].Can J Microbiol,1997,43:103-121.
[154]Rojas A,H01gLG,Glick B R,et al.Synergism between Phyllobacterium sp.(N2 er) and B Juslieheniformis(Pliar),bothfrom a semiaridmar gmve pllere[J].FEMS Microbid Ecol,2001,35:181-187.
[155]BashanY,Moreno M,Troyo E.Growth Dromotion ofthe seawa.tergated oilseed halophyte Salico rnia bigelovii inoculated with mangrove rIliz06phere bacteria and halotoleram Aosrarinum spp[J].Bjol Fertil Soils,2000,32:265-272.
[156]Glick B R.The enhancement of plant growth by free living bacteria[J].Can J Microbiol,1995,41:109-117.
[157]Bashan Y,Moreno M,Tmyo E.Growth promotion of the seawaterirrigated oilseed halophyte Salicornia bigelovii inoculated with mangrove rhizosphere bacteria and halotolerant Azospirilhtm spp[J].Bid Fcrtil Soils,2000,32:265-272.
[158]熊礼明,史瑞和.蒸汽灭菌的土壤对植物的毒害作用及VA菌根的减毒效应[J].土壤学报,1994(31增)：234-239.
[159]Wang G-W (王桂文),‘'H.Y(李海鹰)2003.Primary investigation on radicular endophytes of mangrove at Qinzhou Bay in Guangxi[J].Guangxi Forestry Science(广西林业科学),32(3):121-124(in Chinese).
[160]Wang G-W(王桂文)," H.Y(李海鹰),Sun W.B(孙文波)2003.Primary study on arbuscular mycorrhizas of mangrove in Qinzhou Bay[J].Guihaia(广西植物),23(5):445-449(in Chinese).
[161]张炳欣,张平,陈晓斌.影响引入微生物根部定殖的因素[J].应用生态学报,2000,11(6)：951-953.
[162]陈晓斌,张炳欣.植物根围促生细菌(PGPR)作用机制的研究进展[J].微生物学杂志,2000,20(1)：38-42.
[163]Bashan Y (1998) Inoculants of plant growth-promoting bacteria for use in agriculture[J]. Biotechnol Adv 16:729-770.
[164]Han J G,Yah M H,Sun L,et al.Isolation,Selection,Characterization of Plant Growth-Promoting Bacteria Associated with Rice and Capsicum Plants[R].The 2nd International Workshop and Product Exhibition Oil White Agriculture(Microbial Agriculture).Beijing,2004.
[165]赵小荣,林启美.微生物解磷的研究进展[J].土壤肥料.2001(3)：7-11.
[166]LeanDR S,LiaoC FH,MushyTP,et al.Theimportance ofnitrogen fLxation in lakes:Environmental mle of nitrogen-fLxing bluegreen algae and a symbiotic bacteria[J].Ecol Bull(Stockholm),1978.26:41-51
[167]万璐,康丽华,廖宝文,等.红树林根际解磷菌分离、培养及解磷能力的研究[J].林业科学研究,2004,17(1)：89-94.
[168]Mann F D,Biological nitrogen fixation associated with Blue green algal com unities in the Beaehwood Mangrove Natu Reserve 2.Seasonal variation in acetylene reduction activity[J].SAFRJBOT/S-AFR TydBkr Plantkd.,1993,59(3): 1-8.
[169]Toledo G,Bashan Y.Soeldner A.Cyanobacteria and black mangroves in Northwestern Mexico:colonization and diurnal and seasonalnitrogen fixtation on aerial roots[J].Can J Micmbiol,1995,41:999-1011.
[170]Bashan Y,Hoguin G.Azospirillum-plant relationships:environmental and physiological advances(1990-1996)[J].Can J Micmbiol,1997,43:103-121.
[2]林鹏.中国红树林研究进展[J].厦门大学学报,2001,Q938：592-603.
[3]王幼珊,张美庆等.VA菌根真菌抗盐碱菌株筛选[J].土壤学报,1994(31增)：79-83.
[4]陈华癸.土壤微生物学[M].上海：上海科学技术出版社,1981.
[5]Lynch JM,Whipps JM.Substrate flow in the rhizosphere[J].Plant Soil,1990, 129:1-10.
[6]Hartmann A,Schmid M,Wenzel W,Hinsinger Ph.Rhizosphere 2004-Perspectives and Challenges-ATribute to Lorenz Hiltner[J].Munich,Germany:GSF-National Research Center for Environment and Health,2005.
[7]Kowalchuk GA,Buma DS,de Boer W, Klinkhamer PGL, van Veen JA. Effects of above-ground plant species composition and diversity on the diversity of soil-borne microorganisms[M]. Anton.Leeuw.Int.J.G.,2002,81:509-520.
[8]Zhang FS,Shen JB,Li L,Liu XJ.An overview of rhizosphere processes related with plant nutrition in majorcropping systems in China[J].Plant Soil,2004,260: 89-99.
[9]Johansson JF,Paul LR,Finlay RD.Microbial interactions in the mycorrhizosphere and their significance for sustainable agriculture[J].FEMS Microbiol.Ecol.,2004, 48(1):1-13.
[10]Preston GM. Plant perceptions of plant growth-promotingpseudomonas. Philosophic. Trans.Royal Soc.London Seri[J]. Biol.Sci.,2004,359(1446):907-918.
[11]Ramette A,Frapolli M,Defago G, Moenne-Loccoz Y.Phylogeny of HCN ynthase-encoding hcnBC genes in biocontrol fluorescent pseudomonads and its relationship with host plant species and HCN synthesis ability[M]. Molecul.Plant-Micro.Interact.,2003,16(6):525-535.
[12]Kksepper,Leong T,Tdntze M,et al.l980.Enhanced plant growth siderphones produced by plant growth-promoting rhizobacteria[J].Nature,286:885-886.
[13]Lalitha B.1989.Phytotoxin produced by Diaporthe phaseolorumvar.caulivora,the causal organism of stern canker of soybean [J].Phytopathology,79:499-504.
[14]Long W.W(龙伟文),Wang P(王平),Feng X.M(冯新梅)2000.Research progress on PGPR AMF interactions[J].AppZ Ecol(应用生态学报),11(2):311-314(in Chinese).
[15]PenroseDM an dGlickBR.2003.Methodsforisolatingand charac.terizing ACC deaminase-containing plant growth-promoting rhizobacteria[J].Phsiol Plantarum, 118:10-15.
[16]RamamoorthyV,Visw'anathan R,Raguchander T,et al.2001.Induction of systemic resistance by plantgrowth promoting rhizobac.teriain crop plains against pestsand diseases[J].CtopProuc,20:1-11.
[17]Ramamoorthy V,Raguchan der T and Samiyappan R.2002.En.hancing resistance of tomato and hot peppertoPythium diseases by seed treatment with Fluorescent pseudomonads[J].Eut'o J knt PathoZ.108:429-441.
[18]Rangeshwaran R,W asnikar AR,Prased PD,et al.2002.Isolation of endophyfic bacteria for biological control of wilt pathogens[J].BiolControl.16:125-134.
[19]Ryu CM,Farag MA,Hu CH,et al.2003.Bacterial volatiles promote growth in Arabidopsis[J]. Proc Natl Acad Sci USA,100:4927-4932.
[20]Silva H and Sandro A.2003.Development of a root colonization bioassay for rapid screening of rhizobacteria for potential bioeontrol agents[J]. Phytopathol.151:42-46.
[21]Sc hippers B,Bakker AW,Ba ckker PA.1987.Interactions of delete, rious and beneficial rhizosphere microoganisms[J].Annu Rev Phytopathol,25:339-358.
[22]Snders, RE., Tinker, P.B.1973. phosrhato flow into my-corrhizal root. Pestic.Sci[J].1973(4):385-395.
[23]Paxfitt, R.L..1979, Plant and soil[M].53:55-56.
[24]刘润进,李晓林.丛枝菌根及其应用[M],科学出版社,2000.
[25]RICE RONALD W.DATNOFF LAWRENCE E. RAID RICHARDN,et al. Influence of vesicular-arbuscular mycorrhizae on celery transplant growth and phosphorus use efficiency[J].Journal of Plant Nutrition,2002,25(8):1839-1853.
[26]吴铁,覃照宇等.VA菌根及其对土壤中磷的作用[J].湖南农业科学,2005,(2)：41-43.
[27]赵俊兴,郭柏寿等.VA菌根与植物的磷素营养[J].山东农业大学学报,2001.32(3)：401-405.
[28]李晓林,姚青.VA菌根与植物的矿质营养[J].自然科学进展,2000(6)：524-531.
[29]曾曙才,苏志尧等.VA菌根真菌对植物养分吸收与传递的影响[J].西南林学院学报.2005(1)：72-74.
[30]郑伟文,宋亚娜.VA菌根真菌和根瘤菌对翼豆生长、固氮的影响[J].福建农业学报,2000,15(2)：50-55.
[31]Barea Jm,Anderadeg,Binciotidv,et al.Arbuscular mycorrhizas in sustainable soil plant systems[A].BHOCK,AVARMA.Mycorrhiza stureutre,function,molecular biology and biotechnology[C].Springer-Verlag,Heidelberg,Ger-many,1995.521-559.
[32]Tarafdar J c.Effect of vesicular-arbuscular mycorrhizal and phosphatase-producing fungal inoculation on growth and nutrition of white clover supplied with organic phosphorus[J].Folia Microbiologica,1995,40(3):327-332.
[33]易时来,温明霞.VA茵根改善植物磷素营养的研究进展[J].中国农学通报,2004(5)：164-166.
[34]林先贵,郝文英等.VA菌根对植物耐旱、涝能力的影响[J].土壤,1992.24(3)：142-145.
[35]Subramanian,K. S. and Charest, C. Arbuscular mycorrhizae and nitrogen assimilation in maize after drought and recovery [J]. Physiologiu Plantarum,1998, 102(2):285-296.
[36]赵士杰,李树林.VA菌根促进韭菜增产的生理基础研究[J].土壤肥料,1993(4)：38-40.
[37]王幼珊,张美庆等.VA菌根真菌抗盐碱菌株筛选[J].土壤学报,1994(31增)：79-83.
[38]Feng Gu et al Effect of arbuscular mycorrhizal fungus on the growth of under Nacl stress condition[A],In:Ula.A J.et al (eds).2nd lctl Conf on Mycro[C].Uppsala, Sweden.1998.61.
[39]熊礼明,史瑞和.蒸汽灭菌的土壤对植物的毒害作用及VA菌根的减毒效应[J].土壤学报,1994(31增)：234-239.
[40]Marx D H,Ohio J.Science.[C],1975(75):288-297.
[41]弓明钦,陈应龙,仲祟禄.茵根研究及应用[M].北京：中国林业出版社,1997.
[42]王曙光,林先贵等.丛枝菌根与植物的抗逆性.生态学杂志[J].2001,20(3)：27-30.
[43]KLOEPPER J W,LIFSH1TA R,ZABLOTOWICA R M.Free-living Bacterial Inoculation for Enhancing Crop Productivity[J].Trends in Biotechn,1989,7: 39-43.
[44]BACKER J0,COOKR J.Role of Sidemphoresin Suppression of Pythium Speciesand Production of Increased-growth Response of Wheat by Fluoescent Pseudomonads[J].Phytopathology,1988,78:778-782.
[45]OOSTENDORP M,SIKORA R A.Seed Treatment with Antagonisitic Rhizobacteria for the Suppression of Heterodera Schachtii Early Root Infection of Sugar Beet[J].Revue de Nematologie,1989,12(1):77-83.
[46]RACKE J,SIKORA R A.Isolation,Formulation and Antagonlstc Activity of Rhizobacteria Toward the Potato Cyst Nematode Globodera Pallida[J].Soil Biology and Biochemistry,1992,24(6):521-526.
[47]CHANWAY C P.Endophytes:They're not Just Fungi[J].Can.J.Bot.,1996,74: 321-322.
[48]葛诚主编.微生物肥料的生产应用及其发展,中国农业科技出版社,1996.
[49]吴建峰,林先贵.土壤微生物在促进植物生长方面的作用.土壤.2003,35(1)：18-21.
[50]BROADBENTP,et al.Bacteria andActinomycetesAntagonistic to Fungal Root Pathogensin AustralianSoil[J].Aust J Biol Sci,1971,24:925-944.
[51]WELLER D M.Biological Control of Soil Borne Pathogens in the Rhizosphere with Bacteria[J].Annu Rev phytopathol,1988,(26):379-407.
[52]王金生,赵立平,冯家勋.重组植物病害防治微生物[c]//黄大,林敏.农业微生物基因工程.北京：科学出版社,2001,316-408.
[53]Brockwell J, Bottomley P J.Soil Biol and Biochem,1995,27:683-697.
[54]Rodriguez-Navarro D N, Temprano F, Orvive R.Soil Biol and Biochem[C], 1991,23:375-379.
[55]刘惠琴,宁国赞,管南珠.土壤通报,1994,24(3)：144-145.
[56]Temprano F J, Albareda M, Camacho M,et al. Int Microbiol[M],2002,5:81-86.
[57]周本新,凌世海,尚鹤言等.农药新剂型.化学工业出版社,1994,405-420.
[58]徐莉,杨红,彭建新等.荧光增白剂对苏云金杆菌毒力的增效作用及其紫外防护功效的研究.中国病毒学.2000,15：244.
[59]A.Lamprecht,U.F.Schaefer,C.-M.Lehr.Characterization of microcapsules by confocal laser scanning microscopy:structure, capsulewall composition and encapsulation rate[J]. European Journal of Pharmaceutics and Biopharmaceutics. 2000,49:1-9.
[60]梁治齐.微胶囊化技术及应用[M].北京：中国轻工业出版社.1999.
[61]李玉新,敖聪聪.微胶囊技术及农药微胶囊剂[J].农药.1998,3(7)：4-7.
[62]Patrick B,OD onnell,JamesW.McGinity.Preparation of microspheres by the solvent evaporation technique[J].Advanced Drug DeliveryReviews.1997,28: 25-42.
[63]Anna Shulkin, Harald D.H.SP ver.Polymer microcapsules by interfacial poly addition between styrene-maleic anhydride copolymers and amines[J].Journal of Membrane Science.2002,209:421-432.
[64]Y.F.Fan, X.X.Zhang, X.C.Wang, J.L,i Q.B.Zhu.Super cooling prevention of microencapsulated phase change material[J]. There much in ica A cta.2004, 413:1-6.
[65]袁青梅,杨红卫,杨恺等.微胶囊农药新剂型[J].云南大学学报(自然科学版).2002,24(1 A)：95-97.
[66]史宁,崔光华.流化床包衣制备微囊技术的发展及应用[J].国外医学药学分册.200229(6)：336-340.
[67]K.Dewettinck, A.HuyghebaertFluidized bed coating in food Technology[J].Trends in Food Science& Technology.1999,10:163-168.
[68]栾庆书.外生菌根真菌应用技术研究现状[J].辽宁林业科技.2002(3,4)：67-69.
[69]张亚莲.微生物肥料的功效及在茶树上的利用[J].茶叶通讯.2003,(1)：19-22.
[70]田永辉.不同茶园群落茶树根际微生物群落生态特征[J].茶叶通讯.2001(#)：24-28.
[71]刘柏玉,雷泽周.VA菌根对茶苗生长及养分吸收的影响[J].中国茶叶.1995(5)：6-7.
[72]张亚莲.微生物肥料的功效及在茶树上的利用[J].茶叶通讯.2003,(1)：19-22.
[73]林松,王香甩,宋维希.光合菌肥在茶树上的应用[J].福建茶叶.2002,(2)：22.
[74]叶水娟.茶园施用酵素菌肥料初步试验.中国茶叶[J].2004,26(1)：40-41
[75]许允文、韩文炎、石元值等.茶园施用多效有机菌肥的试验效果[J].中国茶叶.2001,23(4)：12-14.
[76]郭秀珍等编著,1989.内生菌根、林木菌根及应用技术[M].中国林业出版社,80-131.
[77]万水林,1992.柑桔菌根容器育苗技术研究[M].江西农业科技,(6)：26-28.
[78]林鹏.1997.中国红树林生态系[M].北京：科学出版社,23-30.
[79]Jones EBG, Hyde KD.1988. Methods for the study of marine fungi from the mangroves [A]. In:Agate AD, eds. Mangrove Microbiology:Role of Microorganism in Nutrient Cycling of Mangrove Soils and Waters [C]. Paris UNDP/UNESCO,9-27.
[80]Zhuang T,Cheng L P.1998. Soil microbial function of Kandelia candel mangrove: degradation of diesel oil [A]. In:Morton B,ed. The Marine Biology of the South China Sea[C]. Hongkong:Hongkong University Press,389-395.
[81]Alongi DM.1988.Bacterial productivity and microbial biomass in tropical mangrove sediments[J]. Microb. Ecol.,15:59-79.
[82]曾会才‘,郑服丛,贺春萍.2001.海南红树林生境中海疫霉种的分离与鉴定[J]菌物系统,20(3)：310-315.
[83]Zuberer DA,Silver WS.1978. Biological dinitrogen fixation (Acetylene reduction) associated with Florida mangroves[J].Appl.Environ. Microbiol.,35:567-575.
[84]Zuberer DA,Silver WS.1979. N2-fixation (acetylene reduction)and the microbial colonization of mangrove roots[J]. New Phytol.,82:467-471.
[85]Sengupta A,Chaudhuri S.1990. Halotolerant Rhizobium strains from mangrove swamps of the Ganges River Delta[J]. Indian J.Microbiol.,30:483-484.
[86]Holguin QBashan Y,Mendoza2Salgado RA, et al.1999. Microbiology of mangroves,forests in the frontier between land and sea[J].Ciencia Desarrollo,25 (144):26-35.
[87]郑志成,周美英,姚炳新.1989.红树林根系放线菌的组成[J].厦门大学学报(自然科学版),28(3)：306-310.
[88]Bashan Y,Puente ME,Myrold DD, et al.1998. In vitro transfer of fixed nitrogen from diazotrophic filamentous cyanobacteria to black mangrove seedlings [J] FEMS Microbiol. Ecol.,26 (3):165-170.
[89]Ellison AM, Farnsworth EJ, Twilley RR.1996. Facultative mutualism between red mangrove and root2fouling sponges in Belizean mangal[J]. Ecology,77 (8):2431-2444.
[90]Toledo G,Bashan Y,Soeldner A.1995. In vitro colonization and increase in nitrogen fixation of seedling roots of black mangrove inoculated by a filamentous cyanobacteria[J]. Can. Microbiol.,41 (11):1012-1020.
[91]van der Valk AG,Attiwill PM.1984. Acetylene reduction in an A vicennia mari na community in southern Australia [J]. A ust J. Bot.,32:157-164.
[92]Zuberer DA,Silver WS.1978. Biological dinitrogen fixation (Acetylene reduction) associated with Florida mangroves[J].Appl.Environ. Microbiol.,35:567-575.
[93]Vazquez P,Holguin G,Puente ME, et al.2000. Phosphate2solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon [J]. Biol. Fertil. Soils,30:460-468.
[94]Saxena D,Loka2Bharathi PA,Chandramohan D.1988. Sulfate reducing bacteria from mangrove swamps of Goa,central west coast of India[J]. Indian J. Mar. Sci.,17:153-157.
[95]Vethanayagam RR.1991. Purple photosynthetic bacteria from a tropical mangrove environment [J]. Mar. Biol.,110:161-163.
[96]Vethanayagam RR, Krishnamurthy K.1995. Studies on anoxygenic photosynthetic bacterium Rhodopseudomonas sp. from the tropical mangrove environment [J]. Indian J. Mar. Sci.,24:19-23.
[97]Chandrika V,Nair PVR, Khambhadkar LR.1990. Distribution of phototrophic thionic bacteria in the anaerobic and microaerophilic strata of mangrove ecosystem of Cochin [J]. Mar.Biol. Assoc. India,32:77-84.
[98]Dhevendaran K.1984. Photosynthetic bacteria in the marine environment at Porto2Novo [J]. Fish Technol. Soc. Cochi n.,21:126-130.
[99]Shoreit AAM,E12Kady IA,Sayed WF.1994. Isolation and identification of purple nonsulfur bacteria of mangal and non2mangal vegetation of Red Sea Coast,Egypt [J]. Limnologica,24:177-183.
[100]Gotto JW,Taylor BF.1976. N2 fixation associated with decaying leaves of the red mangrove (Rhizophora mangle) [J]. A ppl.Envi ron. Microbiol.,31 781-783.
[101]Mohanraju R,Rajagopal BS,Daniels L, et al.1997. Isolation and characterization of a methanogenic bacteriumfrom mangrove sediments[J]. Mar. Biotechnol.,5:147-152.
[102]Raghukumar S, Sathepatak V, Sharma S, et al.1995. Thraustochytrid and fungal component of marine detritus.3. Field studies on decomposition of lesves of the mangrove Rhizopphora apiculata[J]. A quat. Microb. Ecol.,9 (2):117-125.
[103]Hyde KD,Lee SY.1995. Ecology of mangrove fungi and their role in nutrient cycling:What gaps occur in our knowledge[J]. Hydrobiologia,295 (1-3) 107-188.
[104]Haronian D,Lewis A.1991. Element of a unique bacteriorhodopsin neural network architecture[J]. Appl. Optics,30(5):597-608.
[105]Miyasaka T, Koyama K, Itoh I.1992. Science quantum conversion and image detection by a bacteriorhodopsin2based artificial photoreceptor[J].Science,255 (1):342-344.
[106]张永光,李文均,姜成林,等.2002.嗜盐放线菌的研究进展[J].微生物学杂志,22(4)：45-48.
[107]Maltseva.O,Oriel.P..1997.Monitoring of an alkaline 2,4, trichloropheno degrading enrichment culture by DNA fingerprinting methods and isolation of the responsible organism,Haloakaliphilic Nocardioi des sp. strain M6 [J].Appl. Environ. Microbiol.,63:4145-4149.
[108]刘爱民.2002.嗜盐菌的研究进展[J].安徽师范大学学报(自然科学版),25(2)：181-193.
[109]卢青.2000.植物耐盐性的分子生物学研究进展[J].生物学杂志,17(4)9-11.
[110]林鹏.1990.红树林研究论文集(第1集)[C].厦门：厦门大学出版社,23-30.
[111]林鹏,陈荣华.1991.红树林有机碎屑在河口生态系统中的作用[J].生态学杂志,10(2)：45-48.
[112]云南大学生物系.1980.植物生态学[M].北京：人民教育出版社,310-311.
[113]卢昌义,林鹏.1989.两种红树植物叶分解速率的研究[J].厦门大学学报(自然科学版),27(6)：679-683.
[114]Lu CY,Lin P.1990. Studies on liter fall and decomposition of B ruguier sexangula (Lour) poir community on Hainan Island,China[J]. B ull. Mar. Sci.,47 (1):139-148.
[115]Alongi DM.1994. The role of bacteria in nutrient recycling in tropical mangrove and other coastal benthic ecosystems[J]. Hydrobiologia,285:19-32.
[116]Alongi DM,Christoffersen P, Tirendi F.1993. The influence of forest type on microbial2nutrient relationships in tropical mangrove sediments[J]. Ex p. Mar. Biol.Ecol.171:201-223.
[117]庄铁城,林鹏.1992.九龙江口秋茄红树林凋落叶自然分解与落叶腐解微生物的关系[J].植物生态学与地植物学学报,16(1)：17-25.
[118]Kohlmeyer J,Bebout B,Volkmann KB.1995. Decomposition of mangrove wood by marine fungi and teredinids in Belize [J].Mar. Ecol.,16:27-39.
[119]Raghukumar S,Sharma S,Raghukuma C, et al.1994. Thraustochytrid and fungal component of marine detritus.4. Laboratory studies on decomposition of leaves of the mangrove Rhizophora apiculata Blume[J].Ex p. Mar. Biol Ecol.,183 (1):113-131.
[120]Wu RY.1993. Studies on the microbial ecology of Tansui Estuary [J]. Bot. B ull. Acad. Sin.,34(1):13-30.
[121]Bano N,Nisa MU, Khan N, et al.1997. Significance of bacteria in the flux of organic matter in the tidal creeks of the mangrove ecosystem of the Indus river delta, Pakistan [J]. Mar. Ecol.Prog. Ser.,157:1-12.
[122]Alongi DM,Boto KG, Tirendi F.1989. Effect of exported mangrove litter on bacterial productivity and dissolved organic carbon fluxes in adjacent tropical nearshore sediments [J]. Mar. Ecol.Prog. Ser.,56:133-144.
[123]Boto KG,Alongi DM,Nott ALJ.1989. Dissolved organic carbonbacteria interactions at sediment2water interface in a tropical mangrove system[J]. Mar. Ecol.Prog. Ser.,51:243-251.
[124]Stanley SO,Boto KQAlongi DM, et al.1987. Composition and bacterial utilization of free amino acids in tropical mangrove sediments[J]. Mar. Chem,22:13-30.
[125]Day JW J r eds.1989. Microbial ecology and organic detritus in estuaries[A]. In: Day JW J r,eds. Estuarine Ecology [C]. New York John Wiley & Sons, Inc.,257-308.
[126]Gina H,Patricia V,Yoav B.2001. The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems:An overview[J].Biol.Fert.Soils,33(4):265-278.
[127]Rivera2Monroy VH,Twilley RR.1996. The relative role of denitrification and immobilization in the fate of inorganic nitrogen in mangrove sediments (Terminos Lagoon,Mexico) [J]. Limnol.Oceanogr.,41:284-296.
[128]Rivera2Monroy VH,Day WJ, Twilley RR, et al.1995a. Flux of nitrogen and sediment in a fringe mangrove forest in Terminos lagoon,Mexico[J]. Est uar. Coast. S helf Sci.,40:139-160.
[129]Rivera2Monroy VH,Twilley RR,Boustany RG, et al.1995b. Direct denitrification in mangrove sediments in Terminos Lagoon,Mexico[J]. Mar. Ecol.Prog. Ser.,126:97-109.
[130]Tiedje JM.1988. Ecology of denitrification and dissimilatory nitrate reduction to ammonium[A]. In:Zehnder AJB,ed. Biology of Anaerobic Microorganisms [C]. New York:Wiley,179-244.
[131]Jetten MSM,Strous M,van de Pas2Schoonen KT, et al.1998.The anaerobic oxidation of ammonium [J]. FEMS Microbiol.Rev.,22:421-437.
[132]Bano N,Nisa MU, Khan N, et al.1997. Significance of bacteria in the flux of organic matter in the tidal creeks of the mangrove ecosystem of the Indus river delta, Pakistan [J]. Mar. Ecol.Prog. Ser.,157:1-12.
[133]Robertson AI,Daniel PA.1989. Decomposition and the annual flux of detritus from fallen timber in tropical mangrove forests[J]. L imnol. Oceanogr.,34 640-646.
[134]D'Croz L,Del Rosario J, Holness R.1989. Degradation of red mangrove (Rhizophora mangle) leaves in the bay of Panama[J]. Rev. Biol. Trop. 37:101-104.
[135]Odum WE, Heald EJ.1975. Mangrove forests and aquatic productivity[A]. In: Hasler AD,ed. Coupling of Land and Water Systems:Ecological Study No.10 [C]. New York:SpringerVerlag,129-136.
[136]Sengupta A,Chaudhuri S.Ecology of hetemtrophic dinitrogen fixation in the rhlzosphere of rllangrove plant community at the Ganges fiver estuaryinIndia [J].Oeoologia,1991,87:560-564.
[137]Holguin G, Guzman M A,Bashan Y.Two new nitrogen-fixing Bacteria from the rhizosphere of mangrove trees:their isolation,identification and in vitro interaction with rhizosphere Staphylococcussp[J].FEMS Microhiol Ecol,1992, 101:207-216.
[138]Abllgi D M,Christoffersen P,Tirendi F.The influenoe of forest type on microbial-nutrient relationships in tropical m grove sedlmerits[J].Exp Mar Biol Ecol,1993,171:201-223.
[139]Vazquez P,Holguin G,PuenteM E,et al.Phosphate-solubilizing microorganisms associated with the rhizosphere of rllangroves in a semiarid coastal,Bgoon[J].Biol Fertil Soils,2000,30:460-468.
[140]Holguin G,Bashan Y,Mendoza-Salgado R A,et al.La Micmbiologia de los manglares.Bosques en la frontem entre el mar Y la tierrra[J].Cicnciay Desarrollo,1999,1ZM:26-35.
[141]Holguin G, Vazquez P,Bashan Y.The role of sediment microorganisms in the productivity,conservation,and rehabilitation of the mangrove ecosystems:nil overview[J].Biol Fertil Soils,2001,33:265-278.
[142]董俊德,王汉奎,张偬,等.海洋固氯生物多样性及其对海洋生产力的氮、碳贡献[J].生态学报,2002,22(10)：1741-1749.
[143]Van der Valk A G,Attiwill P M.Acetylene reduction in Itll Avcenna marna community in Southem Australia[J].Aust J Bot,1984.32:157-164.
[144]Sengupta A,Chaudhufi S.Ecology of hetemtrophic dinitrogen firmtion in the rhizosphere of mangrove plant community at the Gangesriver estuary in India[J].Oecologia,1991,87:560-564.
[145]Vasqucz P,Holguin G,Pucntc M E,et al.Phosphate-solubilizingmicroorganisms associated with the rhizosphere of mangroves in flsemiarid coastal lagoon[J].Biol Fcail Soils,2000,30:460-468.
[146]Bashan Y,Hoguin G.Azospirillum-plant relationships:environmental and physiological advances(1990-1996)[J].Can J Micmbiol,1997,43:103-121.
[147]TANDON H L S.Fertilizers,Organic Manures,Recyclable Wastes and Bio-fertilizers[M].New Delhi(India):Fertilizer Development and Consulation Organisation,1992.
[148]陈廷伟,葛诚.我国微生物肥料发展趋向[J].土壤肥料,1995,(6)：16-20.
[149]葛诚主编.微生物肥料的生产应用及其发展[M].北京：中国农业科技出版社,1996.
[150]Puente M E,aols.G,e ck BR,et al.Root surface colonization ofblack nlBflgFove seedlings byAzz-pirilhm halopmeferens and Azospirillum brasilensein seawater [J].FEMS Microbiol Ecol,1999,29:283-292.
[151]Toledo G,BashanY,s0|ner A.In vitrocolonization andincrease in nitrogen fix ation of Ilg roots of black nlangrove inoculated by a filamentous cyanobacteria[J].Can J Mierobiol,1995,41:1012-1020.
[152]Bashan Y,Puente M E,Myrold D D,et al.In vitro transf of fixed nitrogen from diazotrophic fdamentous cyanobacteria to black man grove seedlings[J].FEMS Microbiol Ecol,1998,26:165-170.
[153]Bashan Y,Hols.G.Azospirillum-plant relationships environmental and physi gical advances(1990-1996) [M].Can J Microbiol,1997,43:103-121.
[154]Rojas A,H01gLG,Glick B R,et al.Synergism between Phyllobacterium sp.(N2 er) and B Juslieheniformis(Pliar),bothfrom a semiaridmar gmve pllere[J].FEMS Microbid Ecol,2001,35:181-187.
[155]BashanY,Moreno M,Troyo E.Growth Dromotion ofthe seawa.tergated oilseed halophyte Salico rnia bigelovii inoculated with mangrove rIliz06phere bacteria and halotoleram Aosrarinum spp[J].Bjol Fertil Soils,2000,32:265-272.
[156]Glick B R.The enhancement of plant growth by free living bacteria[J].Can J Microbiol,1995,41:109-117.
[157]Bashan Y,Moreno M,Tmyo E.Growth promotion of the seawaterirrigated oilseed halophyte Salicornia bigelovii inoculated with mangrove rhizosphere bacteria and halotolerant Azospirilhtm spp[J].Bid Fcrtil Soils,2000,32:265-272.
[158]熊礼明,史瑞和.蒸汽灭菌的土壤对植物的毒害作用及VA菌根的减毒效应[J].土壤学报,1994(31增)：234-239.
[159]Wang G-W (王桂文),‘'H.Y(李海鹰)2003.Primary investigation on radicular endophytes of mangrove at Qinzhou Bay in Guangxi[J].Guangxi Forestry Science(广西林业科学),32(3):121-124(in Chinese).
[160]Wang G-W(王桂文)," H.Y(李海鹰),Sun W.B(孙文波)2003.Primary study on arbuscular mycorrhizas of mangrove in Qinzhou Bay[J].Guihaia(广西植物),23(5):445-449(in Chinese).
[161]张炳欣,张平,陈晓斌.影响引入微生物根部定殖的因素[J].应用生态学报,2000,11(6)：951-953.
[162]陈晓斌,张炳欣.植物根围促生细菌(PGPR)作用机制的研究进展[J].微生物学杂志,2000,20(1)：38-42.
[163]Bashan Y (1998) Inoculants of plant growth-promoting bacteria for use in agriculture[J]. Biotechnol Adv 16:729-770.
[164]Han J G,Yah M H,Sun L,et al.Isolation,Selection,Characterization of Plant Growth-Promoting Bacteria Associated with Rice and Capsicum Plants[R].The 2nd International Workshop and Product Exhibition Oil White Agriculture(Microbial Agriculture).Beijing,2004.
[165]赵小荣,林启美.微生物解磷的研究进展[J].土壤肥料.2001(3)：7-11.
[166]LeanDR S,LiaoC FH,MushyTP,et al.Theimportance ofnitrogen fLxation in lakes:Environmental mle of nitrogen-fLxing bluegreen algae and a symbiotic bacteria[J].Ecol Bull(Stockholm),1978.26:41-51
[167]万璐,康丽华,廖宝文,等.红树林根际解磷菌分离、培养及解磷能力的研究[J].林业科学研究,2004,17(1)：89-94.
[168]Mann F D,Biological nitrogen fixation associated with Blue green algal com unities in the Beaehwood Mangrove Natu Reserve 2.Seasonal variation in acetylene reduction activity[J].SAFRJBOT/S-AFR TydBkr Plantkd.,1993,59(3): 1-8.
[169]Toledo G,Bashan Y.Soeldner A.Cyanobacteria and black mangroves in Northwestern Mexico:colonization and diurnal and seasonalnitrogen fixtation on aerial roots[J].Can J Micmbiol,1995,41:999-1011.
[170]Bashan Y,Hoguin G.Azospirillum-plant relationships:environmental and physiological advances(1990-1996)[J].Can J Micmbiol,1997,43:103-121.