硝基苯对于大白鼠心脏和肝脏的危害以及维生素C的对抗作用研究
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摘要
目的:观察硝基苯对于大白鼠的血液、心脏和肝脏的影响,探讨维生素C对于硝基苯危害的对抗作用。
实验方法:以大白鼠为实验对象,测量心肌力学指标(左心室收缩压、左心室舒张末期压、左心室最大收缩速率和左心室最大舒张速率)、血液(平均动脉压、红细胞数)和组织生化(心脏的SOD、MDA,肝脏的SOD、MDA)的变化。
实验结果:1.与对照组和溶剂组相比,硝基苯对于大白鼠的心肌力学指标明显呈剂量依赖性影响,左心室最大舒张速率在15mg/kg硝基苯组发生了显著的变化(与对照组相比下降了40.53%),其余的指标均在45mg/kg硝基苯组有显著变化(与对照组比较,左心室收缩压、左心室舒张末期压﹑左心室最大收缩速率分别变化了-27.48%、+77.94%、-63.78%)。随着硝基苯剂量的增加,心肌力学的各个指标变化越来越大。2.与对照组和溶剂组相比,红细胞数的变化尤其显著。在15mg/kg组,红细胞数的数量即呈现明显的下降(与对照组相比,下降了8.39%)。随着硝基苯剂量的增加,红细胞数的下降越来越大,呈一种剂量依赖性。动脉平均压在45mg/kg硝基苯组呈现出明显的下降(与对照组相比,下降38.5%),此后随着硝基苯剂量的增加,动脉平均压越来越低。3.与对照组和溶剂组相比,心脏和肝脏的MDA在15mg/kg即呈现出明显的变化(与对照组相比,MDA分别增加74.74%和125.03%)。随着硝基苯剂量的增加,MDA则越来越高。心脏和肝脏的SOD在15mg/kg时稍有升高,但是不显著,在45mg/kg硝基苯组SOD开始出现明显下降(与对照组相比,心脏和肝脏的SOD分别下降49.54%和67.45%),随着硝基苯量的增加,SOD越来越低。4.维生素C对抗硝基苯组,除了心脏的SOD有明显的升高外(与45mg/kg硝基苯组相比,45mg/kgNB+0.8g/kgVC组升高了11.56%),其他的各项指标都没有明显的变化。
结论:硝基苯对于心肌力学指标(左心室收缩压、左心室舒张末期压、左心室最大收缩速率和左心室最大舒张速率)、红细胞数、心脏和肝脏的SOD、MDA均呈剂量依赖性的影响。随着硝基苯剂量的增加,左心室舒张末期压、心脏和肝脏的MDA逐渐升高,其他指标则逐渐降低。维生素C对于硝基苯引起的危害作用无明显的对抗。
Objective:To investigate the influence of nitrobenzene on the blood system、heart(the mechanics index of cardiac muscle and the biochemistry trait of tissue)and liver (the biochemistry trait of tissue) ,to explore if the vitamin C antagonize nitrobenzene in rats.
method: In this rat experiments, we measure the changes in myocardial mechanics, biochemistry in blood and tissues. The mechanics index of cardiac muscle include left ventricle shrink pressure ,left ventricle end shrink pressure,+dp/dt,-dp/dt. The index about biochemistry include the activity of superoxide dismutase of heart and liver,the content of malondialdehyde of heart and liver in rats. The index of blood includes mean artery pressure and volume of erythrocyte.
Results: 1.Comparing to the rats in the normal and dimethylsulfoxide group, the mechanics index of cardiac muscle were lower with the increased dose of nitrobenzene,-dp/dt changed observably in 15mg/kg nitrobenzene group(comparing with normal group, it decrease 40.53%), the other indexes changed observably in 45mg/kg nitrobenzene group.( comparing with normal group, left ventricle shrink pressure, left ventricle end diastolic pressure,+ dp/dt decrease、27.48%、77.94、63.78% respectively) 45mg/kg。2.The number of red blood cell reduced rapidly after injecting nitrobenzene , It decreased 8.39% in 15mg/kg nitrobenzene group. the more nitrobenzene ,the less the number of red blood cell. Artery mean pressure decreased distinctly in 45mg/kg nitrobenzene group (comparing with normal group, it decrease 38.5%) ,after this, the artery mean pressure were much lower with the increased dose of nitrobenzene.3. The content of malondialdehyde of heart and liver in rats increased evidently in 15mg/kg nitrobenzene group(it increased 74.74% and 125.03% in heart and liver respectively).when the dose of nitrobenzene increased, the content of malondialdehyde enhanced .the activity of superoxide dismutase of heart and liver increased little in 15mg/kg nitrobenzene group, when the dose of nitrobenzene increased, the activity of superoxide dismutase reduced evidently (in 45mg/kg nitrobenzene group, the activity of superoxide dismutase of heart and liver decreased 49.54% and 67.45% respectively). The more dose of nitrobenzene, the less activity of superoxide dismutase and the more content of malondialdehyde. 4. In the vitamin C against nitrobenzene group, the activity of superoxide dismutase of heart increased (it increased 11.56%), the other indexes had no distinct. change. Conclusion: nitrobenzene had obvious influences on the mechanics of cardiac muscle, the number of erythrocyte, the activity of superoxide dismutase of heart and liver, the content of malondialdehyde of heart and liver in rats. the vitamin C can enhanced the activity of superoxide dismutase of heart, but have no effect to the other indexes.
实验方法:以大白鼠为实验对象,测量心肌力学指标(左心室收缩压、左心室舒张末期压、左心室最大收缩速率和左心室最大舒张速率)、血液(平均动脉压、红细胞数)和组织生化(心脏的SOD、MDA,肝脏的SOD、MDA)的变化。
实验结果:1.与对照组和溶剂组相比,硝基苯对于大白鼠的心肌力学指标明显呈剂量依赖性影响,左心室最大舒张速率在15mg/kg硝基苯组发生了显著的变化(与对照组相比下降了40.53%),其余的指标均在45mg/kg硝基苯组有显著变化(与对照组比较,左心室收缩压、左心室舒张末期压﹑左心室最大收缩速率分别变化了-27.48%、+77.94%、-63.78%)。随着硝基苯剂量的增加,心肌力学的各个指标变化越来越大。2.与对照组和溶剂组相比,红细胞数的变化尤其显著。在15mg/kg组,红细胞数的数量即呈现明显的下降(与对照组相比,下降了8.39%)。随着硝基苯剂量的增加,红细胞数的下降越来越大,呈一种剂量依赖性。动脉平均压在45mg/kg硝基苯组呈现出明显的下降(与对照组相比,下降38.5%),此后随着硝基苯剂量的增加,动脉平均压越来越低。3.与对照组和溶剂组相比,心脏和肝脏的MDA在15mg/kg即呈现出明显的变化(与对照组相比,MDA分别增加74.74%和125.03%)。随着硝基苯剂量的增加,MDA则越来越高。心脏和肝脏的SOD在15mg/kg时稍有升高,但是不显著,在45mg/kg硝基苯组SOD开始出现明显下降(与对照组相比,心脏和肝脏的SOD分别下降49.54%和67.45%),随着硝基苯量的增加,SOD越来越低。4.维生素C对抗硝基苯组,除了心脏的SOD有明显的升高外(与45mg/kg硝基苯组相比,45mg/kgNB+0.8g/kgVC组升高了11.56%),其他的各项指标都没有明显的变化。
结论:硝基苯对于心肌力学指标(左心室收缩压、左心室舒张末期压、左心室最大收缩速率和左心室最大舒张速率)、红细胞数、心脏和肝脏的SOD、MDA均呈剂量依赖性的影响。随着硝基苯剂量的增加,左心室舒张末期压、心脏和肝脏的MDA逐渐升高,其他指标则逐渐降低。维生素C对于硝基苯引起的危害作用无明显的对抗。
Objective:To investigate the influence of nitrobenzene on the blood system、heart(the mechanics index of cardiac muscle and the biochemistry trait of tissue)and liver (the biochemistry trait of tissue) ,to explore if the vitamin C antagonize nitrobenzene in rats.
method: In this rat experiments, we measure the changes in myocardial mechanics, biochemistry in blood and tissues. The mechanics index of cardiac muscle include left ventricle shrink pressure ,left ventricle end shrink pressure,+dp/dt,-dp/dt. The index about biochemistry include the activity of superoxide dismutase of heart and liver,the content of malondialdehyde of heart and liver in rats. The index of blood includes mean artery pressure and volume of erythrocyte.
Results: 1.Comparing to the rats in the normal and dimethylsulfoxide group, the mechanics index of cardiac muscle were lower with the increased dose of nitrobenzene,-dp/dt changed observably in 15mg/kg nitrobenzene group(comparing with normal group, it decrease 40.53%), the other indexes changed observably in 45mg/kg nitrobenzene group.( comparing with normal group, left ventricle shrink pressure, left ventricle end diastolic pressure,+ dp/dt decrease、27.48%、77.94、63.78% respectively) 45mg/kg。2.The number of red blood cell reduced rapidly after injecting nitrobenzene , It decreased 8.39% in 15mg/kg nitrobenzene group. the more nitrobenzene ,the less the number of red blood cell. Artery mean pressure decreased distinctly in 45mg/kg nitrobenzene group (comparing with normal group, it decrease 38.5%) ,after this, the artery mean pressure were much lower with the increased dose of nitrobenzene.3. The content of malondialdehyde of heart and liver in rats increased evidently in 15mg/kg nitrobenzene group(it increased 74.74% and 125.03% in heart and liver respectively).when the dose of nitrobenzene increased, the content of malondialdehyde enhanced .the activity of superoxide dismutase of heart and liver increased little in 15mg/kg nitrobenzene group, when the dose of nitrobenzene increased, the activity of superoxide dismutase reduced evidently (in 45mg/kg nitrobenzene group, the activity of superoxide dismutase of heart and liver decreased 49.54% and 67.45% respectively). The more dose of nitrobenzene, the less activity of superoxide dismutase and the more content of malondialdehyde. 4. In the vitamin C against nitrobenzene group, the activity of superoxide dismutase of heart increased (it increased 11.56%), the other indexes had no distinct. change. Conclusion: nitrobenzene had obvious influences on the mechanics of cardiac muscle, the number of erythrocyte, the activity of superoxide dismutase of heart and liver, the content of malondialdehyde of heart and liver in rats. the vitamin C can enhanced the activity of superoxide dismutase of heart, but have no effect to the other indexes.
引文
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