蜈蚣败毒饮对雌激素周期小鼠阴道上皮细胞有丝分裂的影响
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摘要
目的:蜈蚣败毒饮对雌激素周期小鼠阴道上皮细胞有丝分裂的影响。
方法:取健康昆明雌性小鼠50只,连续3天腹腔注射己烯雌酚,诱导小鼠处于雌激素周期。随机平均分成5组,即蜈蚣败毒饮低剂量组(简称中低组)、蜈蚣败毒饮中剂量组(简称中中组)、蜈蚣败毒饮高剂量组(简称中高组)、复方青黛胶囊组(简称阳性对照组)和生理盐水组。中低组、中中组、中高组分别给予低浓度蜈蚣败毒饮(8.78 g/kg·d)、中浓度蜈蚣败毒饮(17.55g/kg·d)、高浓度蜈蚣败毒饮(35.10 g/kg·d)灌胃,对照组给予复方青黛胶囊悬浊液(0.78 mg/kg·d)灌胃,生理盐水组给予生理盐水(15 ml/kg·d)灌胃。于8:00开始给药,连续7天。基于动物细胞有丝分裂的昼夜节律性,每组实验时间均加以统一。第8天上午10:00给5组动物腹腔注射秋水仙碱15 mg/k g,使细胞有丝分裂周期停滞于有丝分裂中期,便于计数。杀死动物,切取阴道组织,制成病理切片。在光学显微镜下观察并计数基底细胞中所出现的有丝分裂数。
结果:结果表明,生理盐水组基底细胞中有较多的有丝分裂细胞,细胞核增大,深染,有的分叶。阳性对照组和中药各剂量组有丝分裂细胞均有所减少,阳性对照组、中低组、中中组、中高组、与生理盐水组相比P＜0.01;中高组与阳性对照组相比P＜0.05。
结论:蜈蚣败毒饮能抑制小鼠阴道上皮细胞有丝分裂。蜈蚣败毒饮优于复方青黛胶囊。
Objective To observe the affection of WUGONGBAIDUYIN decoction towards the mitosis of vaginal epithelial cells of mouse in the estrogen period.
Methods 50 KM mouse,female,with intraperitoneal injection of Diethylstilbestrol for3 days,are induced in estrogen period.They are divided into 5 groups randomly. Group 1 is treated with WGBDY decoction( 8.78g/kgd), Group 2 is treated with WGBDY decoction(17.5 5 g/kg·d) ,Group 3 is treated with WGBDY decoction ( 35.10g/kg·d) ,Group4 is treated with suspension of FUFANGQINGDAI capsule, Group 5 is treated with NS.All the medicine are given by gavage,at 8Am,for 7 days.On the 8~(th) day,at 10 Am, all the 5 groups are treated with Colchicine by intraperitoneal injection 15mg/kg, to make the mitosis stagnated in the Medium-term for count.Kill the mouse,cut the vaginal organizations,and make the pathological section.Observe the cells in mitosis among the basal cells with optical microscope and count.
Results In group 5,there are more cells in mitosis. Nucleus increase and deep.In other groups,the amount of cells in mitosis decrease.When group 1,2,3,4 are compared with group 5, P<0.01.When group 3 is compared with group 4, P<0.05.
Conclusion WGBDY decoction inhibit the mitosis of vaginal epithelial cells of mouse.
方法:取健康昆明雌性小鼠50只,连续3天腹腔注射己烯雌酚,诱导小鼠处于雌激素周期。随机平均分成5组,即蜈蚣败毒饮低剂量组(简称中低组)、蜈蚣败毒饮中剂量组(简称中中组)、蜈蚣败毒饮高剂量组(简称中高组)、复方青黛胶囊组(简称阳性对照组)和生理盐水组。中低组、中中组、中高组分别给予低浓度蜈蚣败毒饮(8.78 g/kg·d)、中浓度蜈蚣败毒饮(17.55g/kg·d)、高浓度蜈蚣败毒饮(35.10 g/kg·d)灌胃,对照组给予复方青黛胶囊悬浊液(0.78 mg/kg·d)灌胃,生理盐水组给予生理盐水(15 ml/kg·d)灌胃。于8:00开始给药,连续7天。基于动物细胞有丝分裂的昼夜节律性,每组实验时间均加以统一。第8天上午10:00给5组动物腹腔注射秋水仙碱15 mg/k g,使细胞有丝分裂周期停滞于有丝分裂中期,便于计数。杀死动物,切取阴道组织,制成病理切片。在光学显微镜下观察并计数基底细胞中所出现的有丝分裂数。
结果:结果表明,生理盐水组基底细胞中有较多的有丝分裂细胞,细胞核增大,深染,有的分叶。阳性对照组和中药各剂量组有丝分裂细胞均有所减少,阳性对照组、中低组、中中组、中高组、与生理盐水组相比P＜0.01;中高组与阳性对照组相比P＜0.05。
结论:蜈蚣败毒饮能抑制小鼠阴道上皮细胞有丝分裂。蜈蚣败毒饮优于复方青黛胶囊。
Objective To observe the affection of WUGONGBAIDUYIN decoction towards the mitosis of vaginal epithelial cells of mouse in the estrogen period.
Methods 50 KM mouse,female,with intraperitoneal injection of Diethylstilbestrol for3 days,are induced in estrogen period.They are divided into 5 groups randomly. Group 1 is treated with WGBDY decoction( 8.78g/kgd), Group 2 is treated with WGBDY decoction(17.5 5 g/kg·d) ,Group 3 is treated with WGBDY decoction ( 35.10g/kg·d) ,Group4 is treated with suspension of FUFANGQINGDAI capsule, Group 5 is treated with NS.All the medicine are given by gavage,at 8Am,for 7 days.On the 8~(th) day,at 10 Am, all the 5 groups are treated with Colchicine by intraperitoneal injection 15mg/kg, to make the mitosis stagnated in the Medium-term for count.Kill the mouse,cut the vaginal organizations,and make the pathological section.Observe the cells in mitosis among the basal cells with optical microscope and count.
Results In group 5,there are more cells in mitosis. Nucleus increase and deep.In other groups,the amount of cells in mitosis decrease.When group 1,2,3,4 are compared with group 5, P<0.01.When group 3 is compared with group 4, P<0.05.
Conclusion WGBDY decoction inhibit the mitosis of vaginal epithelial cells of mouse.
引文
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[3]张志礼.张志礼皮肤病临床经验辑要〔M〕.北京:中国医药科技出版社,2002.
[4]蒋蔚,赵蔚.从风寒湿论治银屑病105例〔J〕辽宁中医杂志,2001,9(28):548.
[5]刘皙.寻常型银屑病从风湿热辨治的体会〔J〕新中医,2001,2(33):64-65.
[6]郝平生,高子平.中医临床辨治银屑病几点体会〔J〕四川中医,2003,3(21):7-8.
[7]王萍,张凡,邓丙戌,等.张志礼中医辨证治疗银屑病方法及临床研究〔J〕中国中西医结合皮肤性病学杂志,2004,4(3):191-193.
[8]黄敏.刘瓦利主任医师治疗寻常型银屑病的辨证用药经验〔J〕山西中医杂志,2006,1(7):35-36.
[9]周德瑛.脏腑辨证治疗银屑病经验〔J〕中国中医急症,2005,11(14):1080-1085.
[10]潘莉虹.导师刘复兴治疗银屑病经验〔J〕云南中医中药杂志,2007,3(28):4-5.
[11]周萌,陈会茹,陶林昌.血府逐瘀汤治疗寻常型银屑病30例临床观察〔J〕北京中医药大学学报(中医临床版),2005,5(12):14-16.
[12]陈忠春.天花粉汤治疗寻常性银屑病66例〔J〕四川中医,2004,2(22):73-74.
[13]孔瑞龙.“银屑平汤”治疗银屑病110例临床观察〔J〕江苏中医药,2004,8(25):24-25.
[14]王宝玲.白虎汤加减治疗银屑病40例〔J〕陕西中医,2006,5(27):550-551.
[15]孙瑞珍.抗银方治疗寻常型银屑病39例疗效观察〔J〕新中医,2004,4(36):44-45.
[16]李东海,查旭山,刘靖,等.凉血疏肝方治疗血热型银屑病38例疗效观察〔J〕新中医,2006,5(38):40-41.
[17]白英华.凉血四根汤治疗血热型银屑病疗效观察〔J〕辽宁中医杂志,2006, 11(33):1433-1434.
[18]王雅娟,石景伟,董西林,等.紫草活血汤治疗银屑病的疗效及对血液流变学的影响〔J〕西安交通大学学报(医学版),2003,3(26):275-296.
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