可降解多孔型丝素蛋白/羟基磷灰石/BMP-2修复兔桡骨骨缺损
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摘要
[目的]探讨可降解多孔型丝素蛋白／羟基磷灰石/BMP-2(SF/HA/BMP-2)复合人工骨的成骨作用及作为骨替代材料的可行性,期望为临床治疗骨缺损提供新的人工骨材料。
[方法]将可降解多空型SF/HA与具有诱导成骨作用的BMP-2复合,构建具有成骨诱导作用的人工骨。将54只新西兰大白兔麻醉生效后,充分显露左侧桡骨中段,造成15mm节段骨缺损。实验组植入SF/HA/BMP-2复合物,实验对照组植入SF/HA材料,空白对照组骨缺损区不植入任何材料。于术后第4、8及12周行X线摄片检查,观察骨缺损修复及骨塑形情况。参照Lane-Sandhu X线评分标准对各组桡骨缺损的骨修复程度评分。标本大体观察并行HE染色及Masson染色组织学观察,比较各组动物的骨修复情况。
[结果]实验组、实验对照组及空白对照组的放射学及组织学检查显示,新骨生成有统计学差异(P<0.05),实验组优于实验对照组优于空白对照组；大体观察及放射学检查证实：实验组兔桡骨缺损完全愈合；实验组对照组缺损处少部分骨愈合；空白对照组缺损基本无骨修复作用,由纤维组织充填。
[结论]SF/HA/BMP-2降解速率适宜,能较快被骨组织取代,有较好的骨缺损修复能力,基本达到现代骨组织工程学的要求。
Objective:To evaluate the bone formation of the biodegradable porous silk fibroin/ hydroxyapatite/bone morphogenetic protein-2 (SF/HA/BMP-2) to heal a segmental bone defect, and to investigate the feasibility of SF/HA/BMP-2 as a bone substitute material. The article hopefully provide a new artificial bone material for clinical bone defects.
Methods:The biodegradable porous SF/HA/BMP-2 was combined with BMP-2 which has induction of bone formation.54 rabbits were randomly divided into 3 groups. A segmental bone defect (15 mm in length) was created at a left radial in each rabbit. SF/HA/BMP-2 was implanted into the bone defects in the experimental group through open operation, SF/HA was implanted into the bone defects in the experimental control group, and nothin implanted into in the blank control group. The curative effect was evaluated by radiographic examination, histology analysis and eyes observation in each group at post-operative 4,8 and 12 week, respectively.
Result:The rate and quality of new bone formation were significantly different in the experimental group, the experimental control group and the blank control group (P<0.05). The segmental bone defects were complete union in the experimental groups, while the defects were partial union in the experimental control groups. In the defects in blank groups, there were no formation of new bone after operation and bone defects were finally filled with fibrous tissue.
Conclusion:SF/HA/BMP-2 has a suitable degradation rate and can be replaced by normal bone tissue appropriately. It has a better ability to repair bone defects, and can meet the requirements of modern bone tissue engineering.
[方法]将可降解多空型SF/HA与具有诱导成骨作用的BMP-2复合,构建具有成骨诱导作用的人工骨。将54只新西兰大白兔麻醉生效后,充分显露左侧桡骨中段,造成15mm节段骨缺损。实验组植入SF/HA/BMP-2复合物,实验对照组植入SF/HA材料,空白对照组骨缺损区不植入任何材料。于术后第4、8及12周行X线摄片检查,观察骨缺损修复及骨塑形情况。参照Lane-Sandhu X线评分标准对各组桡骨缺损的骨修复程度评分。标本大体观察并行HE染色及Masson染色组织学观察,比较各组动物的骨修复情况。
[结果]实验组、实验对照组及空白对照组的放射学及组织学检查显示,新骨生成有统计学差异(P<0.05),实验组优于实验对照组优于空白对照组；大体观察及放射学检查证实：实验组兔桡骨缺损完全愈合；实验组对照组缺损处少部分骨愈合；空白对照组缺损基本无骨修复作用,由纤维组织充填。
[结论]SF/HA/BMP-2降解速率适宜,能较快被骨组织取代,有较好的骨缺损修复能力,基本达到现代骨组织工程学的要求。
Objective:To evaluate the bone formation of the biodegradable porous silk fibroin/ hydroxyapatite/bone morphogenetic protein-2 (SF/HA/BMP-2) to heal a segmental bone defect, and to investigate the feasibility of SF/HA/BMP-2 as a bone substitute material. The article hopefully provide a new artificial bone material for clinical bone defects.
Methods:The biodegradable porous SF/HA/BMP-2 was combined with BMP-2 which has induction of bone formation.54 rabbits were randomly divided into 3 groups. A segmental bone defect (15 mm in length) was created at a left radial in each rabbit. SF/HA/BMP-2 was implanted into the bone defects in the experimental group through open operation, SF/HA was implanted into the bone defects in the experimental control group, and nothin implanted into in the blank control group. The curative effect was evaluated by radiographic examination, histology analysis and eyes observation in each group at post-operative 4,8 and 12 week, respectively.
Result:The rate and quality of new bone formation were significantly different in the experimental group, the experimental control group and the blank control group (P<0.05). The segmental bone defects were complete union in the experimental groups, while the defects were partial union in the experimental control groups. In the defects in blank groups, there were no formation of new bone after operation and bone defects were finally filled with fibrous tissue.
Conclusion:SF/HA/BMP-2 has a suitable degradation rate and can be replaced by normal bone tissue appropriately. It has a better ability to repair bone defects, and can meet the requirements of modern bone tissue engineering.
引文
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