知柏地黄丸剂型改进和质量标准研究
|
摘要
知柏地黄丸来源于清·《医宗金鉴》,为中国药典2005年版一部收载品种,是滋阴清热的经典名方,由熟地黄、山茱萸、山药、牡丹皮、茯苓、知母、黄柏、泽泻等8味中药组成,具有滋阴降火的功效,用于阴虚火旺所致的骨蒸劳热、咽喉肿痛、耳鸣、盗汗、遗精、小便短赤等症。作为中医临床治疗阴虚火旺常用的方剂,知柏地黄丸在临床上一直广有应用,并不断被改为其他剂型的制剂,如知柏地黄浓缩丸、知柏地黄片、知柏地黄胶囊和知柏地黄颗粒等。近年来,随着其临床应用研究的不断深入,知柏地黄丸可单用或与其他药物合用治疗多种疾病,如阴虚火旺型糖尿病、肾病综合征、急性尿路感染等症,都取得了显著的疗效。
药典收载的知柏地黄丸为全部药材未经提取精制的全粉末水蜜丸或蜜丸,丸剂的特点是吸收缓慢,药效持久,节省药材,便于携带和服用,但全粉末丸剂服用剂量较大,在体内崩解较慢,有效物质溶出度较低、吸收较差,发挥药效慢,且药材粉碎的粒度也会影响药效的作用。
泡腾剂是近年来发展起来的新剂型,具有能在体内快速崩解、迅速发挥药效,生物利用度高等优点。为增强知柏地黄丸的药效,提高生物利用度,减少服药量,方便患者用药,本文根据方中各药材中主要成分的理化性质及剂型的特点,选择适宜的提取方法,对知柏地黄丸中全部药材进行了提取精制及剂型改进,创制出知柏地黄泡腾颗粒。
实验中采用水蒸气蒸馏法对牡丹皮进行提取,得到丹皮酚结晶,并用β-环糊精进行包合,通过正交实验优选了丹皮酚的β-环糊精包合条件,确定的最佳包合工艺为使用6倍量的β-环糊精,在50℃超声包合0.5小时;通过正交实验考察了山茱萸和泽泻的乙醇提取条件,得到的最佳提取工艺为使用8倍量的90%的乙醇,加热回流提取两次,每次1小时;通过正交实验考察了知母、黄柏、熟地黄、山药、茯苓等药材的水煎煮提取条件,依据各因素方差分析结果,确定了最佳提取工艺,即加入15倍量的水,煎煮三次,每次1小时。
在确定了方中各药材最佳提取条件的基础上,进行了制剂成型工艺研究。
通过实验考察了稠膏的干燥温度、辅料的种类和用量以及酸碱的比例和用量对制剂质量的影响,确定了制剂的最佳制备工艺,即稠膏在70℃干燥,得到的干膏粉碎成细粉,加入二分之一量的乳糖和糊精(乳糖—糊精1:2)及丹皮酚的β-环糊精包合物,混匀,再加入四分之一量的柠檬酸和碳酸氢钠(柠檬酸—碳酸氢钠2:3),混匀,用适量的无水乙醇制粒,干燥,即制得知柏地黄泡腾颗粒。
为控制药品的内在质量,确保临床用药的安全有效性,通过试验对知柏地黄泡腾颗粒进行了质量标准研究,采用薄层色谱法对制剂中的牡丹皮、山茱萸、黄柏进行定性鉴别,采用高效液相色谱法对山茱萸中主要有效成分马钱苷进行含量测定。定性鉴别和含量测定方法均进行了方法学验证,结果表明,薄层鉴别色谱法呈现斑点清晰,特异性强,可用于该制剂的定性鉴别;含量测定方法简便准确,重现性好,可用于定量控制。
此外,在室温条件和加速条件下,对三批中试样品进行了为期6个月的初步稳定性考察和加速稳定性考察,结果显示室温留样放置及高温、高湿条件下放置的样品各项检测指标合格,样品稳定性较好。
本研究对全粉末的知柏地黄丸处方中的8味药材全部进行了提取,精制成知柏地黄泡腾颗粒,是对大品种老药物进行的二次性开发研究,通过实验研究,确定了与以往全部药材粉碎入药的丸剂或仅部分药材提取的知柏地黄浓缩制剂有着本质区别的知柏地黄泡腾颗粒的制备方法和质量控制方法,丰富了知柏地黄丸处方的用药形式,增加患者用药的选择性和方便性,同时,有关研究填补了国内知柏地黄丸相关领域的空白,对今后类似药物的二次性开发也提供一定的借鉴意义和参考价值。
Zhibai Dihuang Pills, the classical famous drug, used for nourishing yin and purging fire, with the fountain-head of Golden Mirror of Medicine of Manchu Dynasty, were collected into the Chinese Pharmacopoeia (2005 Edition). In the prescription of Zhibai Dihuang Pills, there are eight kinds of ingredients, such as Rhizoma Anemarrhenae, Cortex Phellodendri Chinensis, Radix Rehmanniae Praeparata, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria and Rhizoma Alismatis. Zhibai Dihuang Pills, with the effect of nourishing yin and purging fire, can be used for the treatment of diabetes, Nephrosis syndrome, Uropoiesis infection, and so on. Zhibai Dihuang Pills have been widly used on the clinical treatment to cure yin deficient, and made into other dosage forms of preparations, such as tablets, capsules, granules. In recent years, along with the constant development of the clinical application, the application range of Zhibai Dihuang pills was extended correspondingly.
Zhibai Dihuang honeyed Pills and water-honeyed pills, made with the powder of herbal ingredients without extraction, were collected into the Chinese Pharmacopoeia. The powder pills have the advantages of absorbing slowly, with affects lasting, but they also have the disadvantages of dissoluting too lowerly, absorbing poorly, and the granularity of powder might affect the effect of the pills.
Effervescent preparation is a new dosage form progressed in recent years, with the advantages of quicklier collapsing, quicklier effection, and higher bioavailability, ect.
In order to strengthen the effect of Zhibai Dihuang Pills, heighten their bioavailability and make them more convenient for patients, studies on the improvement of Zhibai Dihuang precedure and quality
control methods were carried out, and a new patent drug--Zhibai Dihuang Effervescent Granules were developed. The herbal contents in the prescription was extracted. Orthogonal test experiment was used to choose the optimum extraction methods.
In order to control its intrinsic quality to assure the clinical safety and effect, studies on the quality standard of Zhibai Dihuang Effervescent Granules were carried out. Thin layer chromatography ( TLC) and high performance liquid chromatography ( HPLC) were used. Cortex Moutan, Fructus Corni, and Cortex Phellodendri Chinensis were identifid by TLC, and the content of loganin in Zhibai Dihuang Effervescent Granules was determined by HPLC. The TLC identification methods were distinct and highly specific; the HPLC quantity determination method was simple, convenient, accurate and reproductable. The chosen analisys methods could be used for the quality control of Zhibai Dihuang Effervescent Granules.
Initial stability test and acceleration stability test were car- ried for six monthes, and the results indicated that the samples had good stability.
The studies on the Zhibai Dihuang Effervescent Granules will enrich Zhibai Dihuang preparations and application, strengthen their effect, enhance the bioavailability and increase selectivity and convenience for the patients.
药典收载的知柏地黄丸为全部药材未经提取精制的全粉末水蜜丸或蜜丸,丸剂的特点是吸收缓慢,药效持久,节省药材,便于携带和服用,但全粉末丸剂服用剂量较大,在体内崩解较慢,有效物质溶出度较低、吸收较差,发挥药效慢,且药材粉碎的粒度也会影响药效的作用。
泡腾剂是近年来发展起来的新剂型,具有能在体内快速崩解、迅速发挥药效,生物利用度高等优点。为增强知柏地黄丸的药效,提高生物利用度,减少服药量,方便患者用药,本文根据方中各药材中主要成分的理化性质及剂型的特点,选择适宜的提取方法,对知柏地黄丸中全部药材进行了提取精制及剂型改进,创制出知柏地黄泡腾颗粒。
实验中采用水蒸气蒸馏法对牡丹皮进行提取,得到丹皮酚结晶,并用β-环糊精进行包合,通过正交实验优选了丹皮酚的β-环糊精包合条件,确定的最佳包合工艺为使用6倍量的β-环糊精,在50℃超声包合0.5小时;通过正交实验考察了山茱萸和泽泻的乙醇提取条件,得到的最佳提取工艺为使用8倍量的90%的乙醇,加热回流提取两次,每次1小时;通过正交实验考察了知母、黄柏、熟地黄、山药、茯苓等药材的水煎煮提取条件,依据各因素方差分析结果,确定了最佳提取工艺,即加入15倍量的水,煎煮三次,每次1小时。
在确定了方中各药材最佳提取条件的基础上,进行了制剂成型工艺研究。
通过实验考察了稠膏的干燥温度、辅料的种类和用量以及酸碱的比例和用量对制剂质量的影响,确定了制剂的最佳制备工艺,即稠膏在70℃干燥,得到的干膏粉碎成细粉,加入二分之一量的乳糖和糊精(乳糖—糊精1:2)及丹皮酚的β-环糊精包合物,混匀,再加入四分之一量的柠檬酸和碳酸氢钠(柠檬酸—碳酸氢钠2:3),混匀,用适量的无水乙醇制粒,干燥,即制得知柏地黄泡腾颗粒。
为控制药品的内在质量,确保临床用药的安全有效性,通过试验对知柏地黄泡腾颗粒进行了质量标准研究,采用薄层色谱法对制剂中的牡丹皮、山茱萸、黄柏进行定性鉴别,采用高效液相色谱法对山茱萸中主要有效成分马钱苷进行含量测定。定性鉴别和含量测定方法均进行了方法学验证,结果表明,薄层鉴别色谱法呈现斑点清晰,特异性强,可用于该制剂的定性鉴别;含量测定方法简便准确,重现性好,可用于定量控制。
此外,在室温条件和加速条件下,对三批中试样品进行了为期6个月的初步稳定性考察和加速稳定性考察,结果显示室温留样放置及高温、高湿条件下放置的样品各项检测指标合格,样品稳定性较好。
本研究对全粉末的知柏地黄丸处方中的8味药材全部进行了提取,精制成知柏地黄泡腾颗粒,是对大品种老药物进行的二次性开发研究,通过实验研究,确定了与以往全部药材粉碎入药的丸剂或仅部分药材提取的知柏地黄浓缩制剂有着本质区别的知柏地黄泡腾颗粒的制备方法和质量控制方法,丰富了知柏地黄丸处方的用药形式,增加患者用药的选择性和方便性,同时,有关研究填补了国内知柏地黄丸相关领域的空白,对今后类似药物的二次性开发也提供一定的借鉴意义和参考价值。
Zhibai Dihuang Pills, the classical famous drug, used for nourishing yin and purging fire, with the fountain-head of Golden Mirror of Medicine of Manchu Dynasty, were collected into the Chinese Pharmacopoeia (2005 Edition). In the prescription of Zhibai Dihuang Pills, there are eight kinds of ingredients, such as Rhizoma Anemarrhenae, Cortex Phellodendri Chinensis, Radix Rehmanniae Praeparata, Fructus Corni, Cortex Moutan, Rhizoma Dioscoreae, Poria and Rhizoma Alismatis. Zhibai Dihuang Pills, with the effect of nourishing yin and purging fire, can be used for the treatment of diabetes, Nephrosis syndrome, Uropoiesis infection, and so on. Zhibai Dihuang Pills have been widly used on the clinical treatment to cure yin deficient, and made into other dosage forms of preparations, such as tablets, capsules, granules. In recent years, along with the constant development of the clinical application, the application range of Zhibai Dihuang pills was extended correspondingly.
Zhibai Dihuang honeyed Pills and water-honeyed pills, made with the powder of herbal ingredients without extraction, were collected into the Chinese Pharmacopoeia. The powder pills have the advantages of absorbing slowly, with affects lasting, but they also have the disadvantages of dissoluting too lowerly, absorbing poorly, and the granularity of powder might affect the effect of the pills.
Effervescent preparation is a new dosage form progressed in recent years, with the advantages of quicklier collapsing, quicklier effection, and higher bioavailability, ect.
In order to strengthen the effect of Zhibai Dihuang Pills, heighten their bioavailability and make them more convenient for patients, studies on the improvement of Zhibai Dihuang precedure and quality
control methods were carried out, and a new patent drug--Zhibai Dihuang Effervescent Granules were developed. The herbal contents in the prescription was extracted. Orthogonal test experiment was used to choose the optimum extraction methods.
In order to control its intrinsic quality to assure the clinical safety and effect, studies on the quality standard of Zhibai Dihuang Effervescent Granules were carried out. Thin layer chromatography ( TLC) and high performance liquid chromatography ( HPLC) were used. Cortex Moutan, Fructus Corni, and Cortex Phellodendri Chinensis were identifid by TLC, and the content of loganin in Zhibai Dihuang Effervescent Granules was determined by HPLC. The TLC identification methods were distinct and highly specific; the HPLC quantity determination method was simple, convenient, accurate and reproductable. The chosen analisys methods could be used for the quality control of Zhibai Dihuang Effervescent Granules.
Initial stability test and acceleration stability test were car- ried for six monthes, and the results indicated that the samples had good stability.
The studies on the Zhibai Dihuang Effervescent Granules will enrich Zhibai Dihuang preparations and application, strengthen their effect, enhance the bioavailability and increase selectivity and convenience for the patients.
引文
[1] 刘立明,刘军.知柏地黄丸的临床应用进展[J].新疆中医药,2002,20(4):72-74.
[2] 李明权.知柏地黄丸源流考[J].中医研究,1997,10(5):56.
[3] 国家药典委员会.中华人民共和国药典 [S].2005 年版一部.北京化学工业出版社,2005:494.
[4] 叶琴,陈良怡,张春光等.知柏地黄丸浸液对大鼠嗜铬细胞内钙的作用[J].华中理工大学学报,2000,28(6):108-110.
[5] 史正刚,潘明明,张士卿.知柏地黄丸对肾上腺皮质激素型肾阴虚幼龄大鼠血浆 CORT、ACTH、CRH 及肾上腺指数和组织学结构的影响[J].中国中医基础医学杂志,2006,12(3):167-171.
[6] 石曾淑,黄静,于青云.知柏地黄丸加味在防治糖尿病中的应用[J].安徽中医临床杂志,1998,10(1):1-2.
[7] 张兰操.知柏地黄汤加减治疗老年干燥综合征[J].吉林中医药,1993, (3):24.
[8] 聂轩,罗保琴.知柏地黄丸治疗单发性甲状腺结节 26 例观察[J].中国中西医结合杂志,1993,13(2):117.
[9] 旭彦.知柏地黄丸新用途[J].医药与保健,2002,(3):17.
[10] 张学顺.知柏地黄丸在男科的应用[J].中成药,1999,21(3):143.
[11] 王黛丽.知柏地黄丸可以治疗皮肤病[J].浙江中医杂志,2006,41(5):293.
[12] 孙淑芝.知柏地黄丸在病毒性肝炎中的应用[J].江西医药, 1998,33(3):164.
[13] 俞长远.知柏地黄丸治疗高血压 40 例疗效观察[J].中成药,1995,22(4):164.
[14] 龚美德.知柏地黄丸(汤)加味治鼻咽癌放疗副作用[J].江西中医药,1992,23(3):61.
[15] 叶平.妇科临床用知柏地黄汤的体会[J].浙江中医杂志,1994,29(10):45.
[16] 段富津.方剂学[M].2003 年版.上海科学技术出版社,2003:130.
[17] 王宏洁.地黄中梓醇变化条件探讨[J].中国中药杂志,1997,22(7):408.
[18] 边宝林.地黄及其炮制品中总糖及几种主要糖的含量测定[J].中国中药杂志,1995,(8):469.
[19] 苗明三.(怀)熟地黄多糖抗氧化作[J].中国中医药信息杂志,2002, (10):32.
[20] 廖洪利,王伟新,赵福胜等.知母化学研究进展[J].药学实践杂志,2005,23(1):12—13.
[21] 倪梁红,秦民坚.知母资源化学及药理研究进展[J].中国野生植物资源,2005,24(4):18—19.
[22] 季德,陆兔林,宋坤.知母研究进展[J].中华实用中西医杂志,2005,18(13):261.
[23] 黄泰康.常用中药成分与药理手册[M].1994 年版.中国医药科技出版社,1994:1588-1589.
[24] 聂继红,王萍,武琳.黄芩、黄柏提取工艺的研究概述[J].新疆中医药,2005,23(1):65.
[25]李方军.牡丹皮化学成分及药理作用研究进展[J].安徽医药,2004,8(1):9—10.
[26] 黄泰康.常用中药成分与药理手册[M].1994 年版.中国医药科技出版社,1994:1043.
[27] 张云升,成晓迅,和健等.牡丹皮中丹皮酚的提取工艺研究[J].河北省科学院学报,2005,22(3)期:49.
[28] 黄泰康.常用中药成分与药理手册[M].1994 年版.中国医药科技出版社,1994:393-396.
[29] 程林,陈斌,蔡宝.正交实验法优选山药多糖得提取工艺[J].中国药物与临,2005,5(9):650.
[30] 黄泰康.常用中药成分与药理手册[M]. 1994 年版.中国医药科技出版社,1994:1388.
[31] 张思访,刘静涵,蒋建勤等.茯苓的化学成分和药理作用及开发利用[J].中华实用中西医杂志,2005,18(2):227—229.
[32] 张璐,刘强.茯苓多糖制备工艺及药理作用研究进展[J].中国实验方剂学杂志,2006,12(4)63.
[33] 戴建子,张志豪,唐蕾等.山茱萸化学成分及药理作用研究进展[J].中国药业,2009,15(2):74.
[34][43] 赵永席,马国营,梁恒.RP-HPLC-ELSD 测定山茱萸中齐墩果酸和熊果酸的含量[J].中成药,2005,27(11):1314.
[35] 朱玉岚,彭国平.泽泻的萜类化学成分研究进展[J].天然产物研究与开发,2006,18:348—351.
[36] 徐晖.泽泻药理作用研究进展[J].湖南中医杂志,2004,20(3):77.
[37] 刘文娟,耿秋菊,王世华等.牡丹皮中丹皮酚提取工艺的研究[J].中华实用中西医杂志,2005 年,18(1):121—122.
[38] 张佳佳.丹皮酚 β-环糊精包合物制备工艺研究[J].中国药业,2005,14(7):55.
[39][40][49]张建军,涂瑶生.正交设计研究丹皮酚包合工艺[J].海峡药学,2005,17(5):6-7.
[41][50][51] 廖正根,平其能,邹红等.丹皮酚 β-环糊精包合物的制备工艺研究[J].中草药,2005 年 6 月,36(6):845.
[42] 周莉莉,赵晓林,王淑敏等.山茱萸化学成分超声提取工艺的研究[J],现代中药研究与实践,2005,19(3):48.
[44] 涂兴明,黄有仲,欧阳毅等.HPLC 法测定山茱萸中熊果酸的含量[J].江西中医学院学报,2005,17(5):40.
[45] 霍文,孙广利,刘鹏.正教试验法优选茯苓多糖提取工艺[J].西北药学杂志,2006,21(1):18.
[46] 王自军,薛梅,边丽.知母中总黄酮和多糖的微波提取与含量测定[J].时珍国医国药,2005,16(7):594-595.
[47][54] 凌静,邹晓华,干新易.聚维酮碘泡腾颗粒的制备和知质量控制[J].中国药师,2005,8(7):617.
[48] 黄启刚.复方益康唑泡腾片的制备及临床应用[J]. 天津药学,2006,18(2):29.
[52] 张明昶,李健,张小勇.双波长薄层扫描法测定知柏地黄丸中盐酸小檗碱的含量[J].贵阳医学院学报,2002,27(6):511-513.
[53] 仇雅静.HPLC 测定治糜灵泡腾片中盐酸小檗碱的含量[J].现代中药研究与实践,2006,20(1):47-48.
[54] 凌静,邹晓华,干新易.聚维酮碘泡腾颗粒的制备和知质量控制[J].中国药师,2005,8(7):617.
[55] 沙士义.左氧氟沙星泡腾片的制备和临床应用[J].中国医院药学杂志,2005,25(5):470.
[56] 胡林水,郑珺,邵胜荣.银杏叶提取物泡腾片的研制及质量考察[J].中药材,2005,28(1):53.
[57] 席美凤.HPLC 法测定知柏地黄颗粒中马钱苷[J].中草药,2006,37(7):1036-1037.
[58] 王诚元.高效液相测定知柏地黄丸中马钱苷含量[J].湖南中医杂志,2006,22(2):82-83.
[2] 李明权.知柏地黄丸源流考[J].中医研究,1997,10(5):56.
[3] 国家药典委员会.中华人民共和国药典 [S].2005 年版一部.北京化学工业出版社,2005:494.
[4] 叶琴,陈良怡,张春光等.知柏地黄丸浸液对大鼠嗜铬细胞内钙的作用[J].华中理工大学学报,2000,28(6):108-110.
[5] 史正刚,潘明明,张士卿.知柏地黄丸对肾上腺皮质激素型肾阴虚幼龄大鼠血浆 CORT、ACTH、CRH 及肾上腺指数和组织学结构的影响[J].中国中医基础医学杂志,2006,12(3):167-171.
[6] 石曾淑,黄静,于青云.知柏地黄丸加味在防治糖尿病中的应用[J].安徽中医临床杂志,1998,10(1):1-2.
[7] 张兰操.知柏地黄汤加减治疗老年干燥综合征[J].吉林中医药,1993, (3):24.
[8] 聂轩,罗保琴.知柏地黄丸治疗单发性甲状腺结节 26 例观察[J].中国中西医结合杂志,1993,13(2):117.
[9] 旭彦.知柏地黄丸新用途[J].医药与保健,2002,(3):17.
[10] 张学顺.知柏地黄丸在男科的应用[J].中成药,1999,21(3):143.
[11] 王黛丽.知柏地黄丸可以治疗皮肤病[J].浙江中医杂志,2006,41(5):293.
[12] 孙淑芝.知柏地黄丸在病毒性肝炎中的应用[J].江西医药, 1998,33(3):164.
[13] 俞长远.知柏地黄丸治疗高血压 40 例疗效观察[J].中成药,1995,22(4):164.
[14] 龚美德.知柏地黄丸(汤)加味治鼻咽癌放疗副作用[J].江西中医药,1992,23(3):61.
[15] 叶平.妇科临床用知柏地黄汤的体会[J].浙江中医杂志,1994,29(10):45.
[16] 段富津.方剂学[M].2003 年版.上海科学技术出版社,2003:130.
[17] 王宏洁.地黄中梓醇变化条件探讨[J].中国中药杂志,1997,22(7):408.
[18] 边宝林.地黄及其炮制品中总糖及几种主要糖的含量测定[J].中国中药杂志,1995,(8):469.
[19] 苗明三.(怀)熟地黄多糖抗氧化作[J].中国中医药信息杂志,2002, (10):32.
[20] 廖洪利,王伟新,赵福胜等.知母化学研究进展[J].药学实践杂志,2005,23(1):12—13.
[21] 倪梁红,秦民坚.知母资源化学及药理研究进展[J].中国野生植物资源,2005,24(4):18—19.
[22] 季德,陆兔林,宋坤.知母研究进展[J].中华实用中西医杂志,2005,18(13):261.
[23] 黄泰康.常用中药成分与药理手册[M].1994 年版.中国医药科技出版社,1994:1588-1589.
[24] 聂继红,王萍,武琳.黄芩、黄柏提取工艺的研究概述[J].新疆中医药,2005,23(1):65.
[25]李方军.牡丹皮化学成分及药理作用研究进展[J].安徽医药,2004,8(1):9—10.
[26] 黄泰康.常用中药成分与药理手册[M].1994 年版.中国医药科技出版社,1994:1043.
[27] 张云升,成晓迅,和健等.牡丹皮中丹皮酚的提取工艺研究[J].河北省科学院学报,2005,22(3)期:49.
[28] 黄泰康.常用中药成分与药理手册[M].1994 年版.中国医药科技出版社,1994:393-396.
[29] 程林,陈斌,蔡宝.正交实验法优选山药多糖得提取工艺[J].中国药物与临,2005,5(9):650.
[30] 黄泰康.常用中药成分与药理手册[M]. 1994 年版.中国医药科技出版社,1994:1388.
[31] 张思访,刘静涵,蒋建勤等.茯苓的化学成分和药理作用及开发利用[J].中华实用中西医杂志,2005,18(2):227—229.
[32] 张璐,刘强.茯苓多糖制备工艺及药理作用研究进展[J].中国实验方剂学杂志,2006,12(4)63.
[33] 戴建子,张志豪,唐蕾等.山茱萸化学成分及药理作用研究进展[J].中国药业,2009,15(2):74.
[34][43] 赵永席,马国营,梁恒.RP-HPLC-ELSD 测定山茱萸中齐墩果酸和熊果酸的含量[J].中成药,2005,27(11):1314.
[35] 朱玉岚,彭国平.泽泻的萜类化学成分研究进展[J].天然产物研究与开发,2006,18:348—351.
[36] 徐晖.泽泻药理作用研究进展[J].湖南中医杂志,2004,20(3):77.
[37] 刘文娟,耿秋菊,王世华等.牡丹皮中丹皮酚提取工艺的研究[J].中华实用中西医杂志,2005 年,18(1):121—122.
[38] 张佳佳.丹皮酚 β-环糊精包合物制备工艺研究[J].中国药业,2005,14(7):55.
[39][40][49]张建军,涂瑶生.正交设计研究丹皮酚包合工艺[J].海峡药学,2005,17(5):6-7.
[41][50][51] 廖正根,平其能,邹红等.丹皮酚 β-环糊精包合物的制备工艺研究[J].中草药,2005 年 6 月,36(6):845.
[42] 周莉莉,赵晓林,王淑敏等.山茱萸化学成分超声提取工艺的研究[J],现代中药研究与实践,2005,19(3):48.
[44] 涂兴明,黄有仲,欧阳毅等.HPLC 法测定山茱萸中熊果酸的含量[J].江西中医学院学报,2005,17(5):40.
[45] 霍文,孙广利,刘鹏.正教试验法优选茯苓多糖提取工艺[J].西北药学杂志,2006,21(1):18.
[46] 王自军,薛梅,边丽.知母中总黄酮和多糖的微波提取与含量测定[J].时珍国医国药,2005,16(7):594-595.
[47][54] 凌静,邹晓华,干新易.聚维酮碘泡腾颗粒的制备和知质量控制[J].中国药师,2005,8(7):617.
[48] 黄启刚.复方益康唑泡腾片的制备及临床应用[J]. 天津药学,2006,18(2):29.
[52] 张明昶,李健,张小勇.双波长薄层扫描法测定知柏地黄丸中盐酸小檗碱的含量[J].贵阳医学院学报,2002,27(6):511-513.
[53] 仇雅静.HPLC 测定治糜灵泡腾片中盐酸小檗碱的含量[J].现代中药研究与实践,2006,20(1):47-48.
[54] 凌静,邹晓华,干新易.聚维酮碘泡腾颗粒的制备和知质量控制[J].中国药师,2005,8(7):617.
[55] 沙士义.左氧氟沙星泡腾片的制备和临床应用[J].中国医院药学杂志,2005,25(5):470.
[56] 胡林水,郑珺,邵胜荣.银杏叶提取物泡腾片的研制及质量考察[J].中药材,2005,28(1):53.
[57] 席美凤.HPLC 法测定知柏地黄颗粒中马钱苷[J].中草药,2006,37(7):1036-1037.
[58] 王诚元.高效液相测定知柏地黄丸中马钱苷含量[J].湖南中医杂志,2006,22(2):82-83.