不同日龄小尾寒羊与滩羊消化器官及其胃消化酶活性的比较研究
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摘要
选择健康的小尾寒羊和滩羊各36只,在相同饲养管理条件下,分别于80日龄、120日龄、160日龄和200日龄,各选取体重相近的9只羊屠宰,比较研究小尾寒羊与滩羊消化器官生长发育的变化规律。研究结果表明:
1羔羊胃的绝对增重,小尾寒羊均大于滩羊;瘤胃净重,小尾寒羊在160日龄时增重极显著(P<0.01),滩羊在120日龄的增重极显著(P<0.01);瓣胃发育,小尾寒羊的瓣胃从32.30g增重到80.07g,滩羊的瓣胃从16.20g增重到55.82g,增重均十分迅速。网胃净重和皱胃净重,两品种均在160日龄时增重明显(P<0.01);
2羔羊胃的相对增重,瘤胃和皱胃占复胃的比例,两品种均呈现下降的趋势,200日龄时,小尾寒羊的瘤胃下降明显(P<0.05),120日龄时滩羊的皱胃下降显著(P<0.05);网胃和瓣胃占复胃的比例,两品种均呈现上升的趋势。80日龄～200日龄的羔羊复胃占体重的变化,小尾寒羊的比例有下降的趋势,在200日龄时显著(P<0.05),滩羊的比例变化不显著(P>0.05)。
3瘤胃:pH值,小尾寒羊小于滩羊(P>0.05),小尾寒羊变化范围为6.26～6.66,滩羊变化范围为6.29～7.02;瘤胃NH3-N浓度,120日龄前,滩羊大于小尾寒羊,差异显著(P<0.05),120日龄之后,两品种的差异不明显(P>0.05)。纤维素酶活性,比较两个品种变化不明显处于相对稳定的水平上(P>0.05);在瘤胃内其它微生物消化酶活性方面,80日龄～160日龄,小尾寒羊均大于滩羊,在试验后期,除胃蛋白酶活性两品种的差异不显著外(P>0.05),淀粉酶和脂肪酶的活性,小尾寒羊均小于滩羊(P<0.01)。
4皱胃:pH值,小尾寒羊大于滩羊(P>0.05),滩羊大多数在pH2.58～2.89之间,小尾寒羊的pH值在80日龄时为3.86,之后降至2.87且趋于稳定;胃内容物中胃蛋白酶方面, 80日龄～160日龄,滩羊均大于小尾寒羊且差异均极显著(P<0.01),在200日龄时,两品种之间的差异不明显(P>0.05)。
Selection of 36 healthy Small-tail-Han sheep and Tan Sheep respectively, kept under the same conditions ,and nine of them similar weight slaughtered were selected at the age of 80 days, 120 days, 160 days and 200 days to comparatively study the trends in the growth and development of the digestive organs in Tan sheep and Small-tail sheep respectively. The results were in the following:
1、lamb stomach absolute weight gain, the two varieties of comparison, the absolute weight of Small-tail Han Sheep stomach is always bigger than Tan Sheep; Rumen absolute weight gain, Small-tail Han Sheep rumen by 160 days weight gain significantly (P<0.01) and Tan Sheep rumen by the 120-day weight gain significantly (P<0.01); Reticulum, weight gain of Tan Sheep and Small-tail Han Sheep both obviously in 160 days(P<0.01); The research of omasum, Small-tail Han Sheep stomach from the flap 32.30g weight gain to 80.07g, Tan Sheep's stomach valve from 16.20 to 55.82g weight gain, weight gain are very rapidly (P<0.01). Abomasum weight gain of the two varieties are significantly when 160 days old (P<0.01).
2、lamb stomach relative weight gain, the ratio of rumen and rennet stomach to complex stomach of the two species showed a downward trend, but the decline of Small-tail Han Sheep rumen of 200 days was reduced significantly (P<0.05), and Tan Sheep 120 days in the rennet was reduced significantly (P<0.05); The Reticulum and the omasum of the complex ratio of the two species showed a rising trend. 80 ~ 200-day-old lamb stomach for weight changes, the proportion of Small-tail Han Sheep there is a downward trend, in 200 days significantly (P<0.05), and Tan Sheep changes in the proportion of non-significant difference (P>0.05).
3、the contents of the rumen pH value, Small-tail Han Sheep is less than Tan Sheep(P>0.05), and changes in the scope of Small-tail Han Sheep is 6.26 to 6.66 and Tan Sheep changes in the range of 6.29 to 7.02; Rumen NH3-N concentration, before the 120-day-old, Tan Sheep bigger than Small-tail Han Sheep and the difference was significant (P<0.05), after 120 days, the two species differences was not significant (P>0.05). Tan Sheep and Small-tail Han Sheep rumen microbial activity changes show that cellulase in a relatively stable level, the two varieties did not change significantly (P>0.05); In the rumen the other microorganisms activities of digestive enzymes, in the pilot 80~160 days early stage, Small-tail Han Sheep were larger than the Tan Sheep, in trial late, with the exception of pepsin activity of the two species, there was no significant difference (P>0.05), the amylase and lipase activity, both Small-tail Han Sheep is less than Tan Sheep (P<0.01).
4、The pH value of rennet content, Small-tail Han Sheep bigger than Tan Sheep (P>0.05), the majority pH of Tan Sheep between 2.58 ~ 2.89, the pH value of small-tail Han Sheep in 80 days at 3.86, followed to above 2.87 and stabilized; stomach contents of pepsin, between 80 to 160 days, Tan Sheep’s value is more than Small-tail Han Sheep and have very significant differences (P<0.01), but in 200 days, The difference between the two species was not significant (P>0.05).
1羔羊胃的绝对增重,小尾寒羊均大于滩羊;瘤胃净重,小尾寒羊在160日龄时增重极显著(P<0.01),滩羊在120日龄的增重极显著(P<0.01);瓣胃发育,小尾寒羊的瓣胃从32.30g增重到80.07g,滩羊的瓣胃从16.20g增重到55.82g,增重均十分迅速。网胃净重和皱胃净重,两品种均在160日龄时增重明显(P<0.01);
2羔羊胃的相对增重,瘤胃和皱胃占复胃的比例,两品种均呈现下降的趋势,200日龄时,小尾寒羊的瘤胃下降明显(P<0.05),120日龄时滩羊的皱胃下降显著(P<0.05);网胃和瓣胃占复胃的比例,两品种均呈现上升的趋势。80日龄～200日龄的羔羊复胃占体重的变化,小尾寒羊的比例有下降的趋势,在200日龄时显著(P<0.05),滩羊的比例变化不显著(P>0.05)。
3瘤胃:pH值,小尾寒羊小于滩羊(P>0.05),小尾寒羊变化范围为6.26～6.66,滩羊变化范围为6.29～7.02;瘤胃NH3-N浓度,120日龄前,滩羊大于小尾寒羊,差异显著(P<0.05),120日龄之后,两品种的差异不明显(P>0.05)。纤维素酶活性,比较两个品种变化不明显处于相对稳定的水平上(P>0.05);在瘤胃内其它微生物消化酶活性方面,80日龄～160日龄,小尾寒羊均大于滩羊,在试验后期,除胃蛋白酶活性两品种的差异不显著外(P>0.05),淀粉酶和脂肪酶的活性,小尾寒羊均小于滩羊(P<0.01)。
4皱胃:pH值,小尾寒羊大于滩羊(P>0.05),滩羊大多数在pH2.58～2.89之间,小尾寒羊的pH值在80日龄时为3.86,之后降至2.87且趋于稳定;胃内容物中胃蛋白酶方面, 80日龄～160日龄,滩羊均大于小尾寒羊且差异均极显著(P<0.01),在200日龄时,两品种之间的差异不明显(P>0.05)。
Selection of 36 healthy Small-tail-Han sheep and Tan Sheep respectively, kept under the same conditions ,and nine of them similar weight slaughtered were selected at the age of 80 days, 120 days, 160 days and 200 days to comparatively study the trends in the growth and development of the digestive organs in Tan sheep and Small-tail sheep respectively. The results were in the following:
1、lamb stomach absolute weight gain, the two varieties of comparison, the absolute weight of Small-tail Han Sheep stomach is always bigger than Tan Sheep; Rumen absolute weight gain, Small-tail Han Sheep rumen by 160 days weight gain significantly (P<0.01) and Tan Sheep rumen by the 120-day weight gain significantly (P<0.01); Reticulum, weight gain of Tan Sheep and Small-tail Han Sheep both obviously in 160 days(P<0.01); The research of omasum, Small-tail Han Sheep stomach from the flap 32.30g weight gain to 80.07g, Tan Sheep's stomach valve from 16.20 to 55.82g weight gain, weight gain are very rapidly (P<0.01). Abomasum weight gain of the two varieties are significantly when 160 days old (P<0.01).
2、lamb stomach relative weight gain, the ratio of rumen and rennet stomach to complex stomach of the two species showed a downward trend, but the decline of Small-tail Han Sheep rumen of 200 days was reduced significantly (P<0.05), and Tan Sheep 120 days in the rennet was reduced significantly (P<0.05); The Reticulum and the omasum of the complex ratio of the two species showed a rising trend. 80 ~ 200-day-old lamb stomach for weight changes, the proportion of Small-tail Han Sheep there is a downward trend, in 200 days significantly (P<0.05), and Tan Sheep changes in the proportion of non-significant difference (P>0.05).
3、the contents of the rumen pH value, Small-tail Han Sheep is less than Tan Sheep(P>0.05), and changes in the scope of Small-tail Han Sheep is 6.26 to 6.66 and Tan Sheep changes in the range of 6.29 to 7.02; Rumen NH3-N concentration, before the 120-day-old, Tan Sheep bigger than Small-tail Han Sheep and the difference was significant (P<0.05), after 120 days, the two species differences was not significant (P>0.05). Tan Sheep and Small-tail Han Sheep rumen microbial activity changes show that cellulase in a relatively stable level, the two varieties did not change significantly (P>0.05); In the rumen the other microorganisms activities of digestive enzymes, in the pilot 80~160 days early stage, Small-tail Han Sheep were larger than the Tan Sheep, in trial late, with the exception of pepsin activity of the two species, there was no significant difference (P>0.05), the amylase and lipase activity, both Small-tail Han Sheep is less than Tan Sheep (P<0.01).
4、The pH value of rennet content, Small-tail Han Sheep bigger than Tan Sheep (P>0.05), the majority pH of Tan Sheep between 2.58 ~ 2.89, the pH value of small-tail Han Sheep in 80 days at 3.86, followed to above 2.87 and stabilized; stomach contents of pepsin, between 80 to 160 days, Tan Sheep’s value is more than Small-tail Han Sheep and have very significant differences (P<0.01), but in 200 days, The difference between the two species was not significant (P>0.05).
引文
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