力竭运动过程中大鼠心血管活性物质与骨骼肌微血管密度变化的研究
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摘要
1.研究目的
为了解力竭性运动过程中大鼠心血管活性物质与骨骼肌微血管密度的变化规律,探讨骨骼肌运动引起的微血管密度改变的机制及长时间运动力竭发生的心血管因素,而进行实验研究,以期为运动实践和理论研究提供更多的科学依据。
2.研究方法
选取雄性wistar大鼠70只,随机分为A组(安静对照组)、B组(运动1h组)、C组(运动2h组)、D组(运动3h组)、E组(运动力竭组)、F组(力竭恢复2h组)、G组(力竭恢复12h组)7组。所有大鼠经8周跑台训练后,进行末次力竭性运动实验。分别测定大鼠血浆中心钠素(ANP)、降钙素基因相关肽(CGRP)、血管紧张素Ⅱ(AngⅡ)、去甲肾上腺素(NE)、血栓素B_2(TXB_2)、6-酮-前列环素F_(1α)(6-K-PGF_(1α))的浓度以及骨骼肌微血管密度(CD)。所有数据经SPSS12.0统计软件进行单因素方差分析,显著性水平为P<0.05。
3.实验结果
3.1 各组心钠素的比较发现,运动3h组极显著高于安静对照组(P<0.01),显著高于运动1h组、运动2h组和力竭恢复12h组(P<0.05);力竭恢复2h组显著高于运动力竭组,极显著高于安静对照组、运动1h组和力竭恢复12h组;运动力竭组显著高于安静对照组。
3.2 各组降钙素基因相关肽的比较发现,力竭恢复12h组极显著高于其它6组:运动2h组显著高于安静对照组,极显著高于运动3h组和运动力竭组;力竭恢复2h组极显著高于安静对照组、运动3h组和运动力竭组;运动1h组显著高于运动力竭组。
3.3 各组血栓素B_2的比较发现,运动2h组极显著高于安静对照组和力竭恢复12h组;力竭恢复2h组显著高于安静对照组和力竭恢复12h组。
3.4 各组6-酮-前列环素F_(1α)的比较发现,除运动1h组外,安静对照组显著低于运动力竭组,极显著低于其它4组;运动3h组显著高于运动2h组和
Objective:The purpose of this study is to investigate the change law of cardiovascular active material and skeleton capillary density, discussing the possible mechanism that in long time exercise arouses the change of skeleton capillary density and law of exercise exhausts, so that offer more science basis for sport practice and theoretical research. Method:70 male wistar mice were randomly divided into A group (peace compare group) , B group (1h exercise group) , C group (2h exercise group) , D group (3h exercise group) , E group (exhaustive exercise group) , F group (exhaustive resume 2h group), G group (exhaustive resume 12h group) 7 groups .each mouse were trained on the animal stage ,after 8 weeks ,they were carried out exhaustive exercise. The content of ANP、 CGRP、 AngⅡ 、 NE、 TXB2、 6-K-PGF1α in plasma and CD were measured. All of the data have been processed by SPSS 12.0 statistical software with ANOVA and significant level is P<0.05. Result:3.1 each comparison of ANP is discovered, D is much higher than A, higher than B、 C、 G. G is higher than E, much higher than A、 B、 C and G. A、 E compared, E is higher than A.3.2 each comparison of CGRP is discovered, G is higher than other groups. C is higher than A, much higher than D、 E. F is much higher than A、 D and E. B、 E is compared, B is higher than E.3.3 each comparison of TXB2 is discovered, C is much higher than A、 G. F is higher than A、 G.3.4 each comparison of 6-K-PGF_(1a) is discovered, A is lower than E, much lower than C、 D、 F、 G. D is higher than C、 G, much higher than B、 E.3.5 each comparison of 6-K-PGF1α/ TXB2 is discovered, D is higher than B,
为了解力竭性运动过程中大鼠心血管活性物质与骨骼肌微血管密度的变化规律,探讨骨骼肌运动引起的微血管密度改变的机制及长时间运动力竭发生的心血管因素,而进行实验研究,以期为运动实践和理论研究提供更多的科学依据。
2.研究方法
选取雄性wistar大鼠70只,随机分为A组(安静对照组)、B组(运动1h组)、C组(运动2h组)、D组(运动3h组)、E组(运动力竭组)、F组(力竭恢复2h组)、G组(力竭恢复12h组)7组。所有大鼠经8周跑台训练后,进行末次力竭性运动实验。分别测定大鼠血浆中心钠素(ANP)、降钙素基因相关肽(CGRP)、血管紧张素Ⅱ(AngⅡ)、去甲肾上腺素(NE)、血栓素B_2(TXB_2)、6-酮-前列环素F_(1α)(6-K-PGF_(1α))的浓度以及骨骼肌微血管密度(CD)。所有数据经SPSS12.0统计软件进行单因素方差分析,显著性水平为P<0.05。
3.实验结果
3.1 各组心钠素的比较发现,运动3h组极显著高于安静对照组(P<0.01),显著高于运动1h组、运动2h组和力竭恢复12h组(P<0.05);力竭恢复2h组显著高于运动力竭组,极显著高于安静对照组、运动1h组和力竭恢复12h组;运动力竭组显著高于安静对照组。
3.2 各组降钙素基因相关肽的比较发现,力竭恢复12h组极显著高于其它6组:运动2h组显著高于安静对照组,极显著高于运动3h组和运动力竭组;力竭恢复2h组极显著高于安静对照组、运动3h组和运动力竭组;运动1h组显著高于运动力竭组。
3.3 各组血栓素B_2的比较发现,运动2h组极显著高于安静对照组和力竭恢复12h组;力竭恢复2h组显著高于安静对照组和力竭恢复12h组。
3.4 各组6-酮-前列环素F_(1α)的比较发现,除运动1h组外,安静对照组显著低于运动力竭组,极显著低于其它4组;运动3h组显著高于运动2h组和
Objective:The purpose of this study is to investigate the change law of cardiovascular active material and skeleton capillary density, discussing the possible mechanism that in long time exercise arouses the change of skeleton capillary density and law of exercise exhausts, so that offer more science basis for sport practice and theoretical research. Method:70 male wistar mice were randomly divided into A group (peace compare group) , B group (1h exercise group) , C group (2h exercise group) , D group (3h exercise group) , E group (exhaustive exercise group) , F group (exhaustive resume 2h group), G group (exhaustive resume 12h group) 7 groups .each mouse were trained on the animal stage ,after 8 weeks ,they were carried out exhaustive exercise. The content of ANP、 CGRP、 AngⅡ 、 NE、 TXB2、 6-K-PGF1α in plasma and CD were measured. All of the data have been processed by SPSS 12.0 statistical software with ANOVA and significant level is P<0.05. Result:3.1 each comparison of ANP is discovered, D is much higher than A, higher than B、 C、 G. G is higher than E, much higher than A、 B、 C and G. A、 E compared, E is higher than A.3.2 each comparison of CGRP is discovered, G is higher than other groups. C is higher than A, much higher than D、 E. F is much higher than A、 D and E. B、 E is compared, B is higher than E.3.3 each comparison of TXB2 is discovered, C is much higher than A、 G. F is higher than A、 G.3.4 each comparison of 6-K-PGF_(1a) is discovered, A is lower than E, much lower than C、 D、 F、 G. D is higher than C、 G, much higher than B、 E.3.5 each comparison of 6-K-PGF1α/ TXB2 is discovered, D is higher than B,
引文
[1] 徐丰彦.人体生理学[M].北京:人民卫生出版社,1989.1604.
[2] 王迪浔.人体病理生理学[M].北京:人民卫生出版社,2002.901-929.
[3] 朱妙章.心血管生理学与临床[M].北京:高等教育出版社,2004.105-130.
[4] 宋文宣.实用心血管病药物治疗学[M].北京:人民卫生出版社,2001.471-489.
[5] D.R.Wilkie.肌肉[M].北京:科学出版社,1981.86-90.
[6] 杨钢.人体内分泌生理及其异常[M].天津:天津科学技术出版社,1980.87.
[7] 杨锡让.实用运动生理学[M].北京:北京体育大学出版社,2003.113.
[8] 王瑞元.运动生理学[M].北京:人民体育出版社,2002.97-102.
[9] 杨锡让.运动生理学进展-质疑与思考[M].北京:北京体育大学出版社,2000.108-113.
[10] 田野.运动生理学高级教程[M].北京:高等教育出版社,2003.345-353.
[11] 何瑞荣.心血管生理学[M].北京:人民卫生出版社,1987.134.
[12] Hasbak P, et al. calcitonin gene-related peptide and adrenomedulin release in humans[J]. Effects of exercise and hypoxia Regul Pept, 2002, 108(2-3): 89.
[13] Lind H, et al. Different levels of sensory neuropeptides (cal-citonin gene-related peptide and substance P) during and. after exercise in man[J]. Clin physiol, 1996, Jan 16(1): 73-82.
[14] Onuoha GN, et al. Neuropeptide secretion in exercise[J]. Neu-Ropeptides, 1998, 32(4): 319-325.
[15] 石幼琪,等.运动员血浆内皮素和降钙素基因相关肽基础水平的调查[J].西安体育学院学报,1999,02:77-78.
[16] 李昭波,等.运动性肥大心脏心肌和血浆降钙素基因相关肽含量变化[J].中国运动医学杂志,1999,01:7-8.
[17] Tanaka H, Shindo M, et al. Effect of acute exercise on Plasma immunoreactiVe atria1 natriuretic factor[J]. Life Sci, 1986, 39 (18): 1685-1693.
[18] Freund BJ Claybaugh JR, Dice MS, et al. Hormonal and vascular fluid responses to maximal exercise in the trained and untrained males[J]. J Appl Physiol, 1987, 63: 669-675.
[19] 郭黎.运动与心钠素[J].沈阳体育学院学报,2000,(3):82-85.
[20] 李维根,高云秋,等.大鼠极量运动后心肌血管紧张素Ⅱ的变化[J].中国运动医学杂志,1994,01:11-14.
[21] 李严冰,等.少年运动员恒定负荷条件下血浆内皮素、心钠素的变化[J].中国运动医学杂志,2000,03:322-323.
[22] Costill DL. Exercise Induced Sodium Conservation:Changes in plasma renin and aldosteronMed[J]. Sci. Sport, 1976, 8: 209-13.
[23] 常芸,孙迎,陈小同,郭军.运动心脏内分泌功能可复性的研究[J].中国运动医学杂志,1999,01:3-7.
[24] 石幼琪,钟振新,等.不同强度、不同方式运动对血浆内皮素的影响[J].北京体育大学学报,2000,03:338-339.
[25] Mehta, et al. The significant of Platelet- vess- e1 wall prostaglandin equilibrium during exercise induced stress[J]. Am Heart J., 1983. 105:895.
[26] Todd ML, et al. Effect of exercise intensity on 6-keto-PGF_(12)、TXB_2 and 6-keto-PGF_(12)/TXB_2 ratios[J]. Thromb Res., 1992, 65:487.
[27] 王恬,等.血栓素、前列环素与运动后蛋白尿生成机制的实验研究[J].中国运动医学杂志,1996,01:39-43.
[28] 史旭萍,王崇宇.竞技运动与猝死[J].齐齐哈尔医学院学报,2002,01:104.
[29] 徐昕,等.我国运动猝死调查研究[J].中国运动医学杂志,1999,02:99-103.
[30] 田牛.微循环方法学[M].原子能出版社,1985,15-81.
[31] 熊正文.过氧化物酶染色增强剂对微血管染色增强作用的对比研究[J].中国组织化学与细胞化学杂志,1997,02:227-229.
[32] 刘洪珍,孔喜良.运动对人体血清一氧化氮含量和一氧化氮合酶活性影响的研究[J].曲阜师范大学学报(自然科学版),2002,02:99-101.
[33] 刘洪珍.运动训练对人体在不同功能状态下血清中NO含量和NOS活性影响的研究[J].中国体育科技,2001,04:30-31.
[34] 李昭波,等.运动性肥大心脏心肌和血浆降钙素基因相关肽含量变化[J].中国运动医学杂志,1999,01:7-8.
[2] 王迪浔.人体病理生理学[M].北京:人民卫生出版社,2002.901-929.
[3] 朱妙章.心血管生理学与临床[M].北京:高等教育出版社,2004.105-130.
[4] 宋文宣.实用心血管病药物治疗学[M].北京:人民卫生出版社,2001.471-489.
[5] D.R.Wilkie.肌肉[M].北京:科学出版社,1981.86-90.
[6] 杨钢.人体内分泌生理及其异常[M].天津:天津科学技术出版社,1980.87.
[7] 杨锡让.实用运动生理学[M].北京:北京体育大学出版社,2003.113.
[8] 王瑞元.运动生理学[M].北京:人民体育出版社,2002.97-102.
[9] 杨锡让.运动生理学进展-质疑与思考[M].北京:北京体育大学出版社,2000.108-113.
[10] 田野.运动生理学高级教程[M].北京:高等教育出版社,2003.345-353.
[11] 何瑞荣.心血管生理学[M].北京:人民卫生出版社,1987.134.
[12] Hasbak P, et al. calcitonin gene-related peptide and adrenomedulin release in humans[J]. Effects of exercise and hypoxia Regul Pept, 2002, 108(2-3): 89.
[13] Lind H, et al. Different levels of sensory neuropeptides (cal-citonin gene-related peptide and substance P) during and. after exercise in man[J]. Clin physiol, 1996, Jan 16(1): 73-82.
[14] Onuoha GN, et al. Neuropeptide secretion in exercise[J]. Neu-Ropeptides, 1998, 32(4): 319-325.
[15] 石幼琪,等.运动员血浆内皮素和降钙素基因相关肽基础水平的调查[J].西安体育学院学报,1999,02:77-78.
[16] 李昭波,等.运动性肥大心脏心肌和血浆降钙素基因相关肽含量变化[J].中国运动医学杂志,1999,01:7-8.
[17] Tanaka H, Shindo M, et al. Effect of acute exercise on Plasma immunoreactiVe atria1 natriuretic factor[J]. Life Sci, 1986, 39 (18): 1685-1693.
[18] Freund BJ Claybaugh JR, Dice MS, et al. Hormonal and vascular fluid responses to maximal exercise in the trained and untrained males[J]. J Appl Physiol, 1987, 63: 669-675.
[19] 郭黎.运动与心钠素[J].沈阳体育学院学报,2000,(3):82-85.
[20] 李维根,高云秋,等.大鼠极量运动后心肌血管紧张素Ⅱ的变化[J].中国运动医学杂志,1994,01:11-14.
[21] 李严冰,等.少年运动员恒定负荷条件下血浆内皮素、心钠素的变化[J].中国运动医学杂志,2000,03:322-323.
[22] Costill DL. Exercise Induced Sodium Conservation:Changes in plasma renin and aldosteronMed[J]. Sci. Sport, 1976, 8: 209-13.
[23] 常芸,孙迎,陈小同,郭军.运动心脏内分泌功能可复性的研究[J].中国运动医学杂志,1999,01:3-7.
[24] 石幼琪,钟振新,等.不同强度、不同方式运动对血浆内皮素的影响[J].北京体育大学学报,2000,03:338-339.
[25] Mehta, et al. The significant of Platelet- vess- e1 wall prostaglandin equilibrium during exercise induced stress[J]. Am Heart J., 1983. 105:895.
[26] Todd ML, et al. Effect of exercise intensity on 6-keto-PGF_(12)、TXB_2 and 6-keto-PGF_(12)/TXB_2 ratios[J]. Thromb Res., 1992, 65:487.
[27] 王恬,等.血栓素、前列环素与运动后蛋白尿生成机制的实验研究[J].中国运动医学杂志,1996,01:39-43.
[28] 史旭萍,王崇宇.竞技运动与猝死[J].齐齐哈尔医学院学报,2002,01:104.
[29] 徐昕,等.我国运动猝死调查研究[J].中国运动医学杂志,1999,02:99-103.
[30] 田牛.微循环方法学[M].原子能出版社,1985,15-81.
[31] 熊正文.过氧化物酶染色增强剂对微血管染色增强作用的对比研究[J].中国组织化学与细胞化学杂志,1997,02:227-229.
[32] 刘洪珍,孔喜良.运动对人体血清一氧化氮含量和一氧化氮合酶活性影响的研究[J].曲阜师范大学学报(自然科学版),2002,02:99-101.
[33] 刘洪珍.运动训练对人体在不同功能状态下血清中NO含量和NOS活性影响的研究[J].中国体育科技,2001,04:30-31.
[34] 李昭波,等.运动性肥大心脏心肌和血浆降钙素基因相关肽含量变化[J].中国运动医学杂志,1999,01:7-8.