颈椎病Ⅰ号胶囊兔模型神经根周围炎症因子抑制作用的实验研究
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摘要
目的:研究颈椎病兔模型在药物的实验干预下神经根局部组织炎症因子的变化,初步探讨颈椎病Ⅰ号胶囊对兔模型神经根局部炎症因子的作用机理,进而研究颈椎病的形成机理,为颈椎病Ⅰ号胶囊治疗颈椎病提供确切的实验依据。
方法:成年健康家兔46只,随机分配至正常对照组、假手术组及造模组,采用彭宝淦、余家阔等造模方法,切断棘上、棘间韧带并持续低头位的方法进行造模,假手术组只切断棘上、棘间韧带,不予低头位处理。术后2月造模成功后,将造模组随机分配至中药治疗组、模型对照组,中药治疗组予以药物进行干预,模型对照组、假手术组及正常对照组予以生理盐水对比,并采用放免疗法测定治疗后2周、4周神经根局部炎症因子前列腺素E_2(PGE_2)、6-酮-前列腺素F_(1a)(6-K-PGF_(1a))、组织胺(HE)含量的变化。
结果:
1.颈椎病Ⅰ号胶囊与模型对照组相比,能有效地抑制兔模型神经根局部炎症因子,(P＜0.01)。
2.采用彭宝淦、余家阔等的造模方法,造模死亡率低(9.09%),检测效度高,经统计学分析,造模组与正常对照组、假手术组有显著差异(P＜0.01)。
结论:
1.颈椎病Ⅰ号胶囊能有效抑制兔模型神经根局部炎症因子,作用确切,与模型对照组、假手术组及正常对照组相比,效果明显。
2.炎症是导致盘源性颈腰痛的主因,神经根型颈椎病的形成与自身免疫性亦有关。
3.彭宝淦、余家阔造模方法是符合颈椎病形成机理的较好的造模方法。
Objective:To study the nerve roots topical inflammatory factor change of cervical spondylosis rabbit model with drug intervention,the mechanism of cervical spondylosis NO.1 capsule for nerve roots topical inflammatory factor of rabbit model were explored to study the pathogenesis of cervical spondysis,which supply experimental basis for cervical spondylosis NO.1 capsule on treatment of cervical spondylosis.
Methods:46 adult rabbits were divided into false operation group, model -making group and normal group,to establish the model by the methods of Peng bao gan and Yu jia kuo's which resect the cervical supraspinous and interspinous ligaments.2 months after the the models were established,The model-making group were divide into medicine group and normal model group,in which the medicine group were intervued by medicine and other groups were intervued by Sodium Chloride.Then the content of rabbits topical nerve roots inflammatory factors such as E_2(PGE_2)、6-K-PGF_(1a)、HE were examined and comparaed by the method of radioimmunity after treated 2w 4w and 6w.
Result:
1.The content of rabbits topical nerve roots inflammatory factors in cervical spondylosis NO.1 capsule group were significantly lower comparaed with normal model group(P＜0.01)
2.The model estebilsh methods of Peng bao gan and Yu jia kuo has lower mortality and high effective power of detection,and has significant difference in statistics comparaed with false operation group and normal group(P＜0.01)
Conclusion:
1.Cervical sponddylosis NO.1 capsule can limit the nerve roots topical inflammatory factor of rabbit model effectively,compared with normal group,the effect is exactly obvious.
2.Inflammation is the main reason of cervicodynia and lumbodynia caused by pathological changes of discus.and also has relationship with autoimmunity
3.The model-making method of Peng bao ganYu jia kuo is a good method witch consistent with the mechanism of cervical spondylosis
方法:成年健康家兔46只,随机分配至正常对照组、假手术组及造模组,采用彭宝淦、余家阔等造模方法,切断棘上、棘间韧带并持续低头位的方法进行造模,假手术组只切断棘上、棘间韧带,不予低头位处理。术后2月造模成功后,将造模组随机分配至中药治疗组、模型对照组,中药治疗组予以药物进行干预,模型对照组、假手术组及正常对照组予以生理盐水对比,并采用放免疗法测定治疗后2周、4周神经根局部炎症因子前列腺素E_2(PGE_2)、6-酮-前列腺素F_(1a)(6-K-PGF_(1a))、组织胺(HE)含量的变化。
结果:
1.颈椎病Ⅰ号胶囊与模型对照组相比,能有效地抑制兔模型神经根局部炎症因子,(P＜0.01)。
2.采用彭宝淦、余家阔等的造模方法,造模死亡率低(9.09%),检测效度高,经统计学分析,造模组与正常对照组、假手术组有显著差异(P＜0.01)。
结论:
1.颈椎病Ⅰ号胶囊能有效抑制兔模型神经根局部炎症因子,作用确切,与模型对照组、假手术组及正常对照组相比,效果明显。
2.炎症是导致盘源性颈腰痛的主因,神经根型颈椎病的形成与自身免疫性亦有关。
3.彭宝淦、余家阔造模方法是符合颈椎病形成机理的较好的造模方法。
Objective:To study the nerve roots topical inflammatory factor change of cervical spondylosis rabbit model with drug intervention,the mechanism of cervical spondylosis NO.1 capsule for nerve roots topical inflammatory factor of rabbit model were explored to study the pathogenesis of cervical spondysis,which supply experimental basis for cervical spondylosis NO.1 capsule on treatment of cervical spondylosis.
Methods:46 adult rabbits were divided into false operation group, model -making group and normal group,to establish the model by the methods of Peng bao gan and Yu jia kuo's which resect the cervical supraspinous and interspinous ligaments.2 months after the the models were established,The model-making group were divide into medicine group and normal model group,in which the medicine group were intervued by medicine and other groups were intervued by Sodium Chloride.Then the content of rabbits topical nerve roots inflammatory factors such as E_2(PGE_2)、6-K-PGF_(1a)、HE were examined and comparaed by the method of radioimmunity after treated 2w 4w and 6w.
Result:
1.The content of rabbits topical nerve roots inflammatory factors in cervical spondylosis NO.1 capsule group were significantly lower comparaed with normal model group(P＜0.01)
2.The model estebilsh methods of Peng bao gan and Yu jia kuo has lower mortality and high effective power of detection,and has significant difference in statistics comparaed with false operation group and normal group(P＜0.01)
Conclusion:
1.Cervical sponddylosis NO.1 capsule can limit the nerve roots topical inflammatory factor of rabbit model effectively,compared with normal group,the effect is exactly obvious.
2.Inflammation is the main reason of cervicodynia and lumbodynia caused by pathological changes of discus.and also has relationship with autoimmunity
3.The model-making method of Peng bao ganYu jia kuo is a good method witch consistent with the mechanism of cervical spondylosis
引文
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3.王拥军,颈椎病发病因素的流行病学研究概况 中医正骨1999,11(3):41-44
4.王冰、段义萍,颈椎病患病特征的流行病学研究 中南大学学报(医学版)2004,29(4):471-474
5.贾连顺,现代颈椎外科学 上海 上海远东出版社,1993;1
6.Shimpei M,Razuo Y,Keiro O,et al.Experimental cervical spondylosisin the mouse.[J].Spine 1991;16:495
7.彭宝淦、侯树勋、吴仕良,颈椎病突出椎间盘组织炎性反应特性的研究 中国脊柱脊髓杂志2002,12(1):38-40
8.张佐伦、刘立成、周东生,脊柱外科手术及并发症学山东科技出版社2002,1:139
9.彭宝淦、贾连顺,腰椎间盘突出症炎症机理研究概述[J].中华外科杂志,1998,36:724-726
10.彭宝淦、贾连川页,椎间盘突出与磷脂酶A_2[J].中国脊柱脊髓杂志1999,9:47
11.彭宝淦、施杞、沈培芝,等.一个新的实验性颈椎病动物模型[J].中国中医骨伤科杂志,1997,5:11-12
12.余家阔、吴毅文、戴先进等.颈椎病生物力学发病机制实验研究[J].安徽医科大学学报,1990,25(1):47-50
13.林石明、许书亮、李兆文、陈联源等 颈痛宁对颈椎间盘髓核炎症介质影响的实验研究[J].中医药学刊,2004,22(11):2043-2046
14.窦淑筠、符云峰 血及组织中组胺的荧光测定法[J].中华学检验杂志,1981.4(2):100
15.沈映军主编 中药药理学 人民卫生出版社 2005.1
16.王起振,等 沈阳药学院学报,1994,11(4):273
17.吕欣然,等 基础与临床,1994,14(4):69
18.李小莉,不同品种蜈蚣的抗炎镇痛作用,中国中药杂志,1996,21(8):498
19.褚婕,等.中药蜈蚣对小鼠免疫功能的影响,天津药学,1994,6(2):23
20.黄长明、童星杰,青少年腰椎间盘突出症研究现状及进展,颈腰痛杂志.2002,23(4):341-342
21.Yasuma T,Koh SM Oknmura T,et nl Histologicchanggs in nging lumbar intervertbrbral discs J Bone Joint Surg,1990.72-A:220-9
22.张佐伦、刘立成、周东生,脊柱外科手术及并发症学,山东科学技术出版社.2002年第一版:136-137
23.彭宝淦、贾连顺 椎间盘退变机理的研究进展 中国矫形外科杂志 2000,4(7):390-392
24.Mochida K,Komori H,Okawa A,et al.Regression of cervicsldisc herniatioa observed on magnetic resonance images[J].Spine,1998,23(9):990-995
25.Bush K,Chaudhuri R,Hillier S,et al.The parhomorphologicchanges that accompany the resolution of cervical radiculopa-thy[J].Spine,1997,22(2):183-187
26.彭宝淦、贾连顺、侯树勋、李增春、宋海涛、陈德玉 颈椎病发病机制的研究 颈腰痛杂志 2001,22(1):1-4
27.Chbak,Andersson GBJ,Masuda K,et al.A New culture system to studyintervirtbral disc in vitro.Spine.1998,18217
28.Kokubun S,Sakurai M,Tanaka Y.Cartilaginous end-plate in cervical disc herniation[J].Spine.1996,4(5):397
29.王绪辉 肢体痹症的造模方法 上海中医药杂志,1986,(3):3-5
30.杜景华、齐立卿、王金榜,骨刺痛消汤对神经根型颈椎病家兔模型血浆前列腺素E_2(PGE_2)的影响河北中医2005,27(2):151-152
31.Kayama S Olmarker K.Larsson K.et al Cultred autol-ogous nucleus pulposus cells induce functional changes inspinal nerve roots[J].Spine,1998,23:2155
32.Marshall LL,Trathewie ER.Chemical irrtation of nerveroot indisc prolapseIaneet [J].Spine.1973,11:320-324
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