罗勒水提取物对血栓闭塞性脉管炎大鼠模型影响的研究
|
摘要
研究罗勒水提取物对血管内皮细胞(VEC)损伤的保护作用及其对血栓闭塞性脉管炎大鼠模型的治疗作用,进一步探讨罗勒水提取物抗血栓形成的部分作用机制。方法:分别采用皮下注射肾上腺素(Adr)法和月桂酸股动脉注射法建立血管内皮细胞损伤和血栓闭塞性脉管炎大鼠模型,以复方丹参片为阳性对照。胸主动脉切片检查VEC损伤大鼠血管内皮受损情况,分别采用放射免疫法、酶联免疫吸附测试法(ELISA)测定VEC损伤大鼠血浆内皮素、血管性假血友病因子和组织型纤溶酶原激活物含量;观察血栓闭塞性脉管炎大鼠的体征变化、血液流变学指标及对血浆血栓素B_2、6-酮-前列腺素F_1a含量和二者比值的影响。结果:罗勒水提物能显著减轻VEC损伤大鼠动脉内皮层的损伤程度,明显降低血浆内皮素、血管性假血友病因子水平,显著升高血浆组织型纤溶酶原激活物水平,与模型组相比有显著性差异;同时能明显改善血栓闭塞性脉管炎大鼠的血液流变学指标,明显降低血栓闭塞性脉管炎大鼠血浆血栓素B_2水平和血栓素B_2/6-酮-前列腺素F_1a比值,且作用强于复方丹参片组。结论:罗勒水提取物对受损血管内皮细胞具有良好的保护作用,对血栓闭塞性脉管炎大鼠模型具有较好的治疗作用,其机制可能与调节内皮细胞的分泌功能,降低血粘度、抗血小板聚积,维持血浆血栓素A_2和前列环素平衡等功能有关。
Objective:To study the protective effect of aqueous extract of ocimum basilicum on endothelial cell injury inducing by adrenaline and the therapeutic effect of that on the rat models with thromboangitis obliterans.Methods:Models of endothelial injury and thromboangitis obliterans were respectively established via pro injecting adrenaline and injecting lauric acid into the femoral artery of rats.The damaged degree of vascular endothelial cell was evaluated by aorta pectoralis pathologic slice.The content of ET, vWF and t-PA in rat plasma was measured by Radioimmunoassay and ELISA;Observed the changes of physical signs,the indexes of hemorheology and the contents of TXB_2 and 6-Keto-PGF_1 a in plasma of thromboangitis obliterans rats following the treatment with aqueous extract of ocimum basilicum.Results:Compared to those of the model group,aqueous extract of ocimum basilicum can significantly lessen the degree of artery endothelial cell injury,significantly lower the content of ET and vWF in endothelial injury rat plasma and increase the content of t-PA.Meanwhile,significantly improved indexes of hemorhelolgy,decreased content of TXB2 and declined ration of T/K. Conclusion:Aqueous extract of ocimum basilicum presented the protective effect on vascular endothelial cell and significant therapeutic effect on experimental thromboangitis obliterans prepared among rats.The possible mechanisms for this effect may be due to improved the excrete function of vascular endothelial cell,improvement in hemorheology and enhanced anti-thrombus function of vessel endothelia.
Objective:To study the protective effect of aqueous extract of ocimum basilicum on endothelial cell injury inducing by adrenaline and the therapeutic effect of that on the rat models with thromboangitis obliterans.Methods:Models of endothelial injury and thromboangitis obliterans were respectively established via pro injecting adrenaline and injecting lauric acid into the femoral artery of rats.The damaged degree of vascular endothelial cell was evaluated by aorta pectoralis pathologic slice.The content of ET, vWF and t-PA in rat plasma was measured by Radioimmunoassay and ELISA;Observed the changes of physical signs,the indexes of hemorheology and the contents of TXB_2 and 6-Keto-PGF_1 a in plasma of thromboangitis obliterans rats following the treatment with aqueous extract of ocimum basilicum.Results:Compared to those of the model group,aqueous extract of ocimum basilicum can significantly lessen the degree of artery endothelial cell injury,significantly lower the content of ET and vWF in endothelial injury rat plasma and increase the content of t-PA.Meanwhile,significantly improved indexes of hemorhelolgy,decreased content of TXB2 and declined ration of T/K. Conclusion:Aqueous extract of ocimum basilicum presented the protective effect on vascular endothelial cell and significant therapeutic effect on experimental thromboangitis obliterans prepared among rats.The possible mechanisms for this effect may be due to improved the excrete function of vascular endothelial cell,improvement in hemorheology and enhanced anti-thrombus function of vessel endothelia.
引文
[1]张表山,邹江,赵丹等.新型抗血小板药氯吡格雷研究进展.化工进展,2003,22(7):689-693.
[2]赵友春,赵淑梅,王革等.第三代溶血栓药物研究进展.药学进展,2004,28(2):72-75.
[3]朱智明,瞿文生.血小板的活化与活化的血小板.医学与哲学,1994,1:24-25.
[4]杨铁骊,血小板抑制剂在冠心病医治中的应用.天中学刊.2001,16(5):49-51.
[5]汪海.以血管内皮细胞为靶标的新型抗血栓天然药物的研究.世界科学技术—中药现代化基础研究,2001,3(6):37-41.
[6]Kelm MA,Nair MG,Strasburg GM,et al.Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimu sanctum Linn[J].Phytomedicine,2000,7(1):7-13.
[7]Singh S,Rehan HM,Majumdar DK.Effect of Ocimum sanctum fixed oil on blood pressure,blood clotting time and pentobarbitone-induced sleeping time[J].Ethnopharmacol,2001,78(2-3):139-143.
[8]Agmyl J C,Obi C I,Gang S S,et al.Hypoglycaemic activity of Ocimum gratissimum in rats[J].Fitoterapis,2001,72:444-446.
[9]Canasoundari A;UmaDevi P,Rao M NA.Protection against radition-induced chromosome damage in mouse bone marrow by Ocimusanctum[J],Mutation Research,1997,373:271-276.
[10]姚蕾,张少艾,王霞平.三种芳香植物的抗氧化性研究[J].香精香料化妆品,2002(4):4-8.
[11]Veronica N,Offiab,Unoma A,et al.Antidiarchoeal effect of Ocimum gratissimum Leaf extract in experimental animals[J].Joumal of Ethnopharmacology,1999,68:327-330.
[12]国家中医药管理局,《中华本草》编委会编,中华本草[M],第七分册,上海上海科学技术出版社,1999,7,6118-6120.
[13]尹锋,胡立宏,楼凤昌.罗勒化学成分的研究.中国天然药物.2004,2(1):20-24.
[14]林蓉,刘俊田,李旭.槲皮素对血管内皮细胞损伤的保护作用.中国循环杂志,2000,(5):304-305.
[15]赵雪英,顾振纶.槲皮素对凝血酶所致的内皮细胞损伤的保护作用研究.中国野生植物资源,1996,(2):6-7.
[16]柳江华.独活的化学成分与药理研究进展.沈阳药学院学报,1994,11(2):143.
[17]J.H.Liu,et al.Inhibitory Effects of Angelica pubescens.f,biserrata on 5-Lipoxygenase and Cyclogenase.Planta Medica,1998:525.
[18]IbadetTohti,MaynurTursun,AnwarUmar,SubatTurdi,Horyatlmin,Nicholas Moore.aqueous extuact of ocimum basilicum L.decrease platelet aggregation induced by ADP and thrombin in vitro and rats arteric-venous shunt thrombosis in vivo.Thrombosis Research 2006,118,733-739.
[19]薛建峰,赵成军,贾如意。血管内皮损伤标志物,医学综述2006,4,12(8):461-463.
[20]Takuya Kobayashi,Yoshio Tahara,Mayumi Matsumoto.et al.Roles of thromboxane A_2 and prostacyclin in the development of atherosclerosis in apoE-deficient mice.J Clin Invest.2004 September 15;114(6):784-794.
[21]J.M.Bosmans,M.M.Kockx,C.J.Vrints.Fibrin(ogen) and von Willebrand Factor Deposition Are Associated With Intimal Thickening After Balloon Angioplasty of the Rabbit Carotid Artery.Arterioscler.Thromb.Vase.Biol.,April 1,1997;17:634-645.
[22]唐丽,许保华,唐文,等。生脉络通颗粒治疗血栓闭塞性脉管炎的作用机理。中国中医药信息杂志,2002,9(1):32-33.
[23]Yoshinori Toshima,Shin-ichi Satoh,Ichiro Ikegaki,Toshio Asano.A New Model of Cerebral Microthrombosis in Rats and the Neuroprotective Effect of a Rho-Kinase Inhibitor.Stroke.2000;31:2245.
[24]朱惠斌,陈懿,葛金文。建立一种脑梗死血瘀证大鼠模型的实验研究。湖南中医药导报,2004,10(6):77-83.
[25]高秀梅,王怡,张萌,等。复方丹参片抗大鼠心肌缺血作用研究[J]。天津中医药。2003,20(1)23-25.
[26]屈彩琴,杨幼新,张远。复方丹参片对大鼠实验性血栓形成的影响。河北中医,1999,21(4):253.
[27]向柏,潘振华,曹德英。复方丹参滴丸质量控制和药理学研究进展。中国药房2007,18(9):708-710.
[28]李雁,周林瑛,姚祖武,等.血管内皮损伤大鼠动物模型的制作[J].福建医科大学学报,2002:36(3):299-301.
[29]Ashida,S.1,Protective ellect of ticlopiclopidine on experimentally induced peripheral arterial occlusive disease in mts.Thromb Res.,1980,18:55.
[30]Hladoec,j.Asensitive model of venous thrombosis in rots.Thromb Res.,1986,43:539
[31]Furchgott,Zaw adzkio The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineo Nature,1980,288:373-376
[32]吴其夏.体液因素和血液循环病理生理.北京:北京医科大学、中国协和医科大学联合出版社,1994,189-195.
[33]谢露,陈蒙华,黎静,等。海带多糖L01对实验性动物血液凝固和血小板活性的影响[J]。中国临床康复,2005;9(5):124-125.
[34]Pober JS,Cotran RC.Cytodines and endothelial cell biology[J].Physio Rev,1990:70:427-451.
[35]Mantovani A,Bussolino F,DejanaE.Cytodine regulation of endothelial cell function[J].FASEBJ,1992;6:2591-2599.
[36]Davies PF,Tdpathi SC.Mechanical stress mechanisms and the cell an endothelial paradigm(Mini-review)[J].CircRes,1993;72:239-45.
[37]Unger T,Ganten D,Lang RE.Is tissue converting enzyme inhibition a determinant of the antihypertensive efficacy If clnverting enzyme inhibitors? Studies with the two different compounds.HOE-498 and MK-421 inspontaneously hypertensive rats.J Cardiovase Pharmacol 1994;6:872-880.
[38]王丽娜,郭旭昌,邓树勋.血管紧张Ⅱ与运动性肾功能异常[J].中国临床康复,2003,7(27):3786.
[39]Yanagisawa M,Kurihara H,Kimura S,Tomobe Y,Kobayashi M,Mitsui Y,Yazaki Y,Goto K,Masaki T.A novel potent vasoconstrictor peptide produced by vascular endothelial cells.Nature.1988;332:411-415.
[40]吴家幂,储照虎.急性脑血管病患者血浆心钠素及内皮素测定的临床意义及相关性研究[J].中华神经科杂志,1998,31(3):1-2.
[41]Galie N,Manes A,Branzi A.The endothelin system in pulmonary arterial hypertension.Cardiovasc Res.2004;61:227-237.
[42]王幕一,洪飞,孙桂芝.血栓病的基础与临床[M].沈阳:辽宁科技出版社.1996,132-136.
[43]卢建敏,王丽霞,高思民.不稳定型心绞痛患者血浆内皮素的改变及低分子量肝素对其影响[J].国外医学心血管疾病分册,2000,27(2):100.
[44]Briede JJ,Wielders SJ,Heemskerk JW,et al.Von Willebrand factor stimulates thrombin-induced exposure of procoagulant phospholipids on the surface of fibrin-adherent platelet[sJ].J Thromb Haemost,2003,(13):559-565.
[45]Vanhoorelbeke K,Ulrichts H,Schoolmeester A,et al.Inhibition of plate-let adhesion to collagen as a new target for antithrombotic drug[sJ].Curr Drug Targets Cardiovasc Haematol Disrod,2003,(32):125-140.
[46]杨爱东,沈庆法,郭永洁,等.清热化瘀方对内毒素血症家兔血管内皮细胞分泌功能的影响.中国中医药信息杂志,2006,13(5):25-27.
[47]Emeis JJ.The control of tPA and PAI-1 secretion from the vessel wall.Vasc Med Rev.1995;6:153-166.
[48]刘政,侯玉芬,周涛,等.消栓通脉颗粒剂对深静脉血栓形成模型大鼠血浆t-PA PAI的影响.中医药学刊,2006,24(10):1846-1848.
[49]Reilly M,Fitz Gerald GA.Cellular activation by thromboxane A_2 and other eicosanoids.Eur Heart J.1993;14:S88-S93.
[50]Reilly IA,Fitz Gerald GA.Aspirin in cardiovascular disease.Drugs.1988;35:154-176.
[51]FitzGerald GA,Smith B,Pederesen AK,Brash AR.Increased prostaeyelin biosynthesis in patients with severe atheroselerosis and platelet activation.N Engl J Med.1984;310:1065-1068.
[52]Fitzgerald DJ,Roy L,Catella F,FitzGerald GA.Platelet activation in unstable coronary disease.N Engl J Med.1986;315:983-989
[53]HJ Weiss and VT Turitto.Prostacyclin(prostaglandin I2,PGI_2) inhibits platelet adhesion and thrombus formation on subendothelium.Blood,Feb 1979;53:244-250.
[54]Pettigrew LC,Grotta.JC,Rhoades HM,et al.Effect of thromboxane synthese inhibition on eicosaanoid levels and blood flow in ischemic rat rain[J].Stroke,1989,20(5):627.
[55]Iwata Y,Kuallya F,Haykama M,et al.Circulating endothelial cells fall to reduce cerebral imfarction in rabbits[J].Stroke,1986,17(3):506-507.
[56]Konieczkowski M,Skrinska VA.Increased synthesis of thromboxane A_2and expression of procoagulant activity by monocytes in response to arachidonic acid in diabetes mellitus[J].Prostaglandins Leukot Essent Fatty Acids,2001,6(53):133.
[57]Kamiya A,Bukhari R,Togawa T.Adaptive regulation of wall shear stress optimizing vascular tree function.Bull Math Biol.1984;46:127-137.
[58]张清,郭京艳.灯盏细辛注射液对血液流变学的影响.临床医药,2007,16(9):47.
[59]贺石林,李俊成,秦晓群.临床生理学[M].北京:科学出版社.2001.87-89.
[1]Iwata Y,Kuallya F,Haykama M,et al.Circulating endothelial cells fall to reduce cerebral imfarction in rabbits[J].Stroke,1986,17(3):506-507.
[2]Pettigrew LC,Grotta.JC,Rhoades HM,et al.Effect of thromboxane synthese inhibition on eicosaanoid levels and blood flow in ischemic rat brain[J].Stroke,1989,20(5):627.
[3]徐晤,陈建辉,王向东,等.灯盏花素注射液对不稳定型心绞痛患者内皮细胞保护作用的观察.基层中药杂志,2001,15(5):5-7.
[4]盛静,徐济民,杨菊闲,等.灯盏细辛对犬急性心肌缺血是血小板聚集功能、TXB_2、6-Keto-PGF_(1a)含量的影响.中华心血管病杂志,1995,23(1):53.
[5]杨辉,李菁,楼一层,等.葛根素治疗老年人高脂血症的疗效及对血栓素A_2和前列环素的影响.中国药师,2002,5(6):362-363.
[6]姜子恩,李金铭,张志秋,等.葛根素对周围血管病血栓素A_2的影响.中国中西医结合外科杂志,2002,8(2):94.
[7]王万铁,沈炳权,金可可,等.葛根素对心肌缺血-再灌注损伤兔乳酸脱氢酶活性变化的影响.中国中西医结合急救杂志,2004,11(6):349-351.
[8]王阶,许军,衷敬柏,等.三七总苷对高黏血症患者血小板活化分子表达和血小板聚集的影响。中国中西医结合杂志,2004,24(4):312-316.
[9]邵凯隽,王万铁,张晓隆,等.三七总皂苷对肺缺血再灌注损伤时氧化应激及TXA_2╱PGI_2失衡的影响.温州医学院学报,2006,36(2):23-25.
[10]胡侦明,劳汉昌,张宝华,等.实验性脊髓损伤早期三七总皂苷对PGI_2和TXB_2的影响.中国脊柱脊髓杂志1995;5(5):206-208.
[11]王卫,王万铁,徐正,等.川芎嗪对家兔肠缺血再灌注损伤时血浆TXA_2/PGI_2水平的影响.温州医学院学报,2004,24(4):256-258.
[12]党胜春,张建新,瞿建国,等.川芎嗪对急性坏死性胰腺炎大鼠胰腺及肾损伤的保护作用.山东医药,2005,45(36):13-14。
[13]王万铁,林丽娜,吴进泽,等.川芎嗪联用异丙酚对围手术期缺血再灌注损伤肝脏的保护作用.中国中西医结合杂志,2006,26(3):205-208。
[14]孔祥英,龚培力.蝙蝠葛酚性碱对血栓形成和血小板聚集的影响[J].药学学报,2005,40(10):916-919.
[15]AsaiA,Miyazawa T.Dietary curcum ino ids prevent high fat diet induced lipid accumulation in rat liver and epididymal adipose tissue.J Nutr,2001;131(11):2932-2935
[16]丁奇峰,陈志强,黄怀鹏,等.姜黄素对大鼠动脉粥样硬化内皮细胞损伤的影响.中药药理与临床,2005;21(5):10-11.
[17]N:Y.Ginkgo biloba extract Protection of brain meurors from olamage induceb by free radicals[M].Illioriois:Aocs Pree Champaign,1996,250-255.
[18]贾玲.银杏叶制剂对老年冠心病患者血栓素及前列环素水平的影响.山东医药,2004,44(5):59-60.
[19]孙萍,王璐,赵萍,等.银杏叶提取物治疗急性脑梗死患者血浆内皮素血栓素、前列环素及血液流变性的研究.贵州医药,2006,30(2):173-172。
[20]孙萍,王璐,赵萍,等.银杏叶提取物治疗急性脑梗死患者血浆内皮素血栓素、前列环素及血液流变性的研究.贵州医药,2006,30(2):173-172.
[21]马世平.刘保林,周素娣,等.西红花总甙的药理研究[J],中草药,1999,30(3):196-198.
[22]高其铭.中药红花的药理研究概论[J].中西医结合杂志,1984,4(12):75-79.
[23]陈发胜,孙丰雷,魏爱生,等.红花注射液治疗糖尿病周围神经病变的机制探讨.中西医结合心脑血管病杂志,2003,1(8):456-458.
[24]王淑君,王万铁,熊建华,等.红花注射液对脑缺血-再灌注损伤家兔血浆TXA_2/PGI_2水平的影响.中国现代应用药学杂志,2003,20(2):101-102.
[25]高健,徐先祥,徐先俊,等.黄芪总皂苷抗血栓形成作用实验研究.中成药,2002,24(2):116.
[26]余静,郭雪娅,赵锋,等.黄芪注射液对高血压病患者血浆P选择素、血栓素B_2及6-Keto-PGF_(1a)的影响.《临床荟萃》 2005,20(13):721-723.
[27]白如君,褚伟,徐洁,等.丹参提取液对糖尿病大鼠血浆ET、TXB_2、6-Keto-PGF_(1a)的影响.湖北中医学院学报,2006,8(3):11-12.
[28]刘久波,陈林,朱少铭,等.丹参注射液对急性肾衰竭兔心肌缺血的保护作用。医药导报,2005,24(11):997-1000.
[29]王梅.丹参对重症急性胰腺炎并发肾功能损害的保护作用.临床药学,2004,13(10):65-66.
[30]何淑虹.丹参对花生四烯酸及血脂调节的大鼠实验研究.浙江中西医结合杂志,2005,15(12):749-750.
[31]肖志华,王家陇.丹参对急性肝损害大鼠体内血栓素、前列环素的作用.同济医科大学学报,1997,26(6);450-452.
[32]张建新,程国祚,李龙,等.丹参对大鼠急性坏死性胰腺炎并发肺损伤的影响.中华急诊医学杂志,2002,11(2):101-104.
[33]彭从健,钟培金,刘和强,等.刺五加注射液对急性脑梗死患者血栓素和前列环素的影响.中国中医急症,2006,15(7):732-733.
[34]睢大员,曲绍春,于小风,等.刺五加叶皂苷对大鼠心肌缺血再灌注损伤的保护作用.中国中药杂志,2004,29(1):71-74.
[2]赵友春,赵淑梅,王革等.第三代溶血栓药物研究进展.药学进展,2004,28(2):72-75.
[3]朱智明,瞿文生.血小板的活化与活化的血小板.医学与哲学,1994,1:24-25.
[4]杨铁骊,血小板抑制剂在冠心病医治中的应用.天中学刊.2001,16(5):49-51.
[5]汪海.以血管内皮细胞为靶标的新型抗血栓天然药物的研究.世界科学技术—中药现代化基础研究,2001,3(6):37-41.
[6]Kelm MA,Nair MG,Strasburg GM,et al.Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimu sanctum Linn[J].Phytomedicine,2000,7(1):7-13.
[7]Singh S,Rehan HM,Majumdar DK.Effect of Ocimum sanctum fixed oil on blood pressure,blood clotting time and pentobarbitone-induced sleeping time[J].Ethnopharmacol,2001,78(2-3):139-143.
[8]Agmyl J C,Obi C I,Gang S S,et al.Hypoglycaemic activity of Ocimum gratissimum in rats[J].Fitoterapis,2001,72:444-446.
[9]Canasoundari A;UmaDevi P,Rao M NA.Protection against radition-induced chromosome damage in mouse bone marrow by Ocimusanctum[J],Mutation Research,1997,373:271-276.
[10]姚蕾,张少艾,王霞平.三种芳香植物的抗氧化性研究[J].香精香料化妆品,2002(4):4-8.
[11]Veronica N,Offiab,Unoma A,et al.Antidiarchoeal effect of Ocimum gratissimum Leaf extract in experimental animals[J].Joumal of Ethnopharmacology,1999,68:327-330.
[12]国家中医药管理局,《中华本草》编委会编,中华本草[M],第七分册,上海上海科学技术出版社,1999,7,6118-6120.
[13]尹锋,胡立宏,楼凤昌.罗勒化学成分的研究.中国天然药物.2004,2(1):20-24.
[14]林蓉,刘俊田,李旭.槲皮素对血管内皮细胞损伤的保护作用.中国循环杂志,2000,(5):304-305.
[15]赵雪英,顾振纶.槲皮素对凝血酶所致的内皮细胞损伤的保护作用研究.中国野生植物资源,1996,(2):6-7.
[16]柳江华.独活的化学成分与药理研究进展.沈阳药学院学报,1994,11(2):143.
[17]J.H.Liu,et al.Inhibitory Effects of Angelica pubescens.f,biserrata on 5-Lipoxygenase and Cyclogenase.Planta Medica,1998:525.
[18]IbadetTohti,MaynurTursun,AnwarUmar,SubatTurdi,Horyatlmin,Nicholas Moore.aqueous extuact of ocimum basilicum L.decrease platelet aggregation induced by ADP and thrombin in vitro and rats arteric-venous shunt thrombosis in vivo.Thrombosis Research 2006,118,733-739.
[19]薛建峰,赵成军,贾如意。血管内皮损伤标志物,医学综述2006,4,12(8):461-463.
[20]Takuya Kobayashi,Yoshio Tahara,Mayumi Matsumoto.et al.Roles of thromboxane A_2 and prostacyclin in the development of atherosclerosis in apoE-deficient mice.J Clin Invest.2004 September 15;114(6):784-794.
[21]J.M.Bosmans,M.M.Kockx,C.J.Vrints.Fibrin(ogen) and von Willebrand Factor Deposition Are Associated With Intimal Thickening After Balloon Angioplasty of the Rabbit Carotid Artery.Arterioscler.Thromb.Vase.Biol.,April 1,1997;17:634-645.
[22]唐丽,许保华,唐文,等。生脉络通颗粒治疗血栓闭塞性脉管炎的作用机理。中国中医药信息杂志,2002,9(1):32-33.
[23]Yoshinori Toshima,Shin-ichi Satoh,Ichiro Ikegaki,Toshio Asano.A New Model of Cerebral Microthrombosis in Rats and the Neuroprotective Effect of a Rho-Kinase Inhibitor.Stroke.2000;31:2245.
[24]朱惠斌,陈懿,葛金文。建立一种脑梗死血瘀证大鼠模型的实验研究。湖南中医药导报,2004,10(6):77-83.
[25]高秀梅,王怡,张萌,等。复方丹参片抗大鼠心肌缺血作用研究[J]。天津中医药。2003,20(1)23-25.
[26]屈彩琴,杨幼新,张远。复方丹参片对大鼠实验性血栓形成的影响。河北中医,1999,21(4):253.
[27]向柏,潘振华,曹德英。复方丹参滴丸质量控制和药理学研究进展。中国药房2007,18(9):708-710.
[28]李雁,周林瑛,姚祖武,等.血管内皮损伤大鼠动物模型的制作[J].福建医科大学学报,2002:36(3):299-301.
[29]Ashida,S.1,Protective ellect of ticlopiclopidine on experimentally induced peripheral arterial occlusive disease in mts.Thromb Res.,1980,18:55.
[30]Hladoec,j.Asensitive model of venous thrombosis in rots.Thromb Res.,1986,43:539
[31]Furchgott,Zaw adzkio The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineo Nature,1980,288:373-376
[32]吴其夏.体液因素和血液循环病理生理.北京:北京医科大学、中国协和医科大学联合出版社,1994,189-195.
[33]谢露,陈蒙华,黎静,等。海带多糖L01对实验性动物血液凝固和血小板活性的影响[J]。中国临床康复,2005;9(5):124-125.
[34]Pober JS,Cotran RC.Cytodines and endothelial cell biology[J].Physio Rev,1990:70:427-451.
[35]Mantovani A,Bussolino F,DejanaE.Cytodine regulation of endothelial cell function[J].FASEBJ,1992;6:2591-2599.
[36]Davies PF,Tdpathi SC.Mechanical stress mechanisms and the cell an endothelial paradigm(Mini-review)[J].CircRes,1993;72:239-45.
[37]Unger T,Ganten D,Lang RE.Is tissue converting enzyme inhibition a determinant of the antihypertensive efficacy If clnverting enzyme inhibitors? Studies with the two different compounds.HOE-498 and MK-421 inspontaneously hypertensive rats.J Cardiovase Pharmacol 1994;6:872-880.
[38]王丽娜,郭旭昌,邓树勋.血管紧张Ⅱ与运动性肾功能异常[J].中国临床康复,2003,7(27):3786.
[39]Yanagisawa M,Kurihara H,Kimura S,Tomobe Y,Kobayashi M,Mitsui Y,Yazaki Y,Goto K,Masaki T.A novel potent vasoconstrictor peptide produced by vascular endothelial cells.Nature.1988;332:411-415.
[40]吴家幂,储照虎.急性脑血管病患者血浆心钠素及内皮素测定的临床意义及相关性研究[J].中华神经科杂志,1998,31(3):1-2.
[41]Galie N,Manes A,Branzi A.The endothelin system in pulmonary arterial hypertension.Cardiovasc Res.2004;61:227-237.
[42]王幕一,洪飞,孙桂芝.血栓病的基础与临床[M].沈阳:辽宁科技出版社.1996,132-136.
[43]卢建敏,王丽霞,高思民.不稳定型心绞痛患者血浆内皮素的改变及低分子量肝素对其影响[J].国外医学心血管疾病分册,2000,27(2):100.
[44]Briede JJ,Wielders SJ,Heemskerk JW,et al.Von Willebrand factor stimulates thrombin-induced exposure of procoagulant phospholipids on the surface of fibrin-adherent platelet[sJ].J Thromb Haemost,2003,(13):559-565.
[45]Vanhoorelbeke K,Ulrichts H,Schoolmeester A,et al.Inhibition of plate-let adhesion to collagen as a new target for antithrombotic drug[sJ].Curr Drug Targets Cardiovasc Haematol Disrod,2003,(32):125-140.
[46]杨爱东,沈庆法,郭永洁,等.清热化瘀方对内毒素血症家兔血管内皮细胞分泌功能的影响.中国中医药信息杂志,2006,13(5):25-27.
[47]Emeis JJ.The control of tPA and PAI-1 secretion from the vessel wall.Vasc Med Rev.1995;6:153-166.
[48]刘政,侯玉芬,周涛,等.消栓通脉颗粒剂对深静脉血栓形成模型大鼠血浆t-PA PAI的影响.中医药学刊,2006,24(10):1846-1848.
[49]Reilly M,Fitz Gerald GA.Cellular activation by thromboxane A_2 and other eicosanoids.Eur Heart J.1993;14:S88-S93.
[50]Reilly IA,Fitz Gerald GA.Aspirin in cardiovascular disease.Drugs.1988;35:154-176.
[51]FitzGerald GA,Smith B,Pederesen AK,Brash AR.Increased prostaeyelin biosynthesis in patients with severe atheroselerosis and platelet activation.N Engl J Med.1984;310:1065-1068.
[52]Fitzgerald DJ,Roy L,Catella F,FitzGerald GA.Platelet activation in unstable coronary disease.N Engl J Med.1986;315:983-989
[53]HJ Weiss and VT Turitto.Prostacyclin(prostaglandin I2,PGI_2) inhibits platelet adhesion and thrombus formation on subendothelium.Blood,Feb 1979;53:244-250.
[54]Pettigrew LC,Grotta.JC,Rhoades HM,et al.Effect of thromboxane synthese inhibition on eicosaanoid levels and blood flow in ischemic rat rain[J].Stroke,1989,20(5):627.
[55]Iwata Y,Kuallya F,Haykama M,et al.Circulating endothelial cells fall to reduce cerebral imfarction in rabbits[J].Stroke,1986,17(3):506-507.
[56]Konieczkowski M,Skrinska VA.Increased synthesis of thromboxane A_2and expression of procoagulant activity by monocytes in response to arachidonic acid in diabetes mellitus[J].Prostaglandins Leukot Essent Fatty Acids,2001,6(53):133.
[57]Kamiya A,Bukhari R,Togawa T.Adaptive regulation of wall shear stress optimizing vascular tree function.Bull Math Biol.1984;46:127-137.
[58]张清,郭京艳.灯盏细辛注射液对血液流变学的影响.临床医药,2007,16(9):47.
[59]贺石林,李俊成,秦晓群.临床生理学[M].北京:科学出版社.2001.87-89.
[1]Iwata Y,Kuallya F,Haykama M,et al.Circulating endothelial cells fall to reduce cerebral imfarction in rabbits[J].Stroke,1986,17(3):506-507.
[2]Pettigrew LC,Grotta.JC,Rhoades HM,et al.Effect of thromboxane synthese inhibition on eicosaanoid levels and blood flow in ischemic rat brain[J].Stroke,1989,20(5):627.
[3]徐晤,陈建辉,王向东,等.灯盏花素注射液对不稳定型心绞痛患者内皮细胞保护作用的观察.基层中药杂志,2001,15(5):5-7.
[4]盛静,徐济民,杨菊闲,等.灯盏细辛对犬急性心肌缺血是血小板聚集功能、TXB_2、6-Keto-PGF_(1a)含量的影响.中华心血管病杂志,1995,23(1):53.
[5]杨辉,李菁,楼一层,等.葛根素治疗老年人高脂血症的疗效及对血栓素A_2和前列环素的影响.中国药师,2002,5(6):362-363.
[6]姜子恩,李金铭,张志秋,等.葛根素对周围血管病血栓素A_2的影响.中国中西医结合外科杂志,2002,8(2):94.
[7]王万铁,沈炳权,金可可,等.葛根素对心肌缺血-再灌注损伤兔乳酸脱氢酶活性变化的影响.中国中西医结合急救杂志,2004,11(6):349-351.
[8]王阶,许军,衷敬柏,等.三七总苷对高黏血症患者血小板活化分子表达和血小板聚集的影响。中国中西医结合杂志,2004,24(4):312-316.
[9]邵凯隽,王万铁,张晓隆,等.三七总皂苷对肺缺血再灌注损伤时氧化应激及TXA_2╱PGI_2失衡的影响.温州医学院学报,2006,36(2):23-25.
[10]胡侦明,劳汉昌,张宝华,等.实验性脊髓损伤早期三七总皂苷对PGI_2和TXB_2的影响.中国脊柱脊髓杂志1995;5(5):206-208.
[11]王卫,王万铁,徐正,等.川芎嗪对家兔肠缺血再灌注损伤时血浆TXA_2/PGI_2水平的影响.温州医学院学报,2004,24(4):256-258.
[12]党胜春,张建新,瞿建国,等.川芎嗪对急性坏死性胰腺炎大鼠胰腺及肾损伤的保护作用.山东医药,2005,45(36):13-14。
[13]王万铁,林丽娜,吴进泽,等.川芎嗪联用异丙酚对围手术期缺血再灌注损伤肝脏的保护作用.中国中西医结合杂志,2006,26(3):205-208。
[14]孔祥英,龚培力.蝙蝠葛酚性碱对血栓形成和血小板聚集的影响[J].药学学报,2005,40(10):916-919.
[15]AsaiA,Miyazawa T.Dietary curcum ino ids prevent high fat diet induced lipid accumulation in rat liver and epididymal adipose tissue.J Nutr,2001;131(11):2932-2935
[16]丁奇峰,陈志强,黄怀鹏,等.姜黄素对大鼠动脉粥样硬化内皮细胞损伤的影响.中药药理与临床,2005;21(5):10-11.
[17]N:Y.Ginkgo biloba extract Protection of brain meurors from olamage induceb by free radicals[M].Illioriois:Aocs Pree Champaign,1996,250-255.
[18]贾玲.银杏叶制剂对老年冠心病患者血栓素及前列环素水平的影响.山东医药,2004,44(5):59-60.
[19]孙萍,王璐,赵萍,等.银杏叶提取物治疗急性脑梗死患者血浆内皮素血栓素、前列环素及血液流变性的研究.贵州医药,2006,30(2):173-172。
[20]孙萍,王璐,赵萍,等.银杏叶提取物治疗急性脑梗死患者血浆内皮素血栓素、前列环素及血液流变性的研究.贵州医药,2006,30(2):173-172.
[21]马世平.刘保林,周素娣,等.西红花总甙的药理研究[J],中草药,1999,30(3):196-198.
[22]高其铭.中药红花的药理研究概论[J].中西医结合杂志,1984,4(12):75-79.
[23]陈发胜,孙丰雷,魏爱生,等.红花注射液治疗糖尿病周围神经病变的机制探讨.中西医结合心脑血管病杂志,2003,1(8):456-458.
[24]王淑君,王万铁,熊建华,等.红花注射液对脑缺血-再灌注损伤家兔血浆TXA_2/PGI_2水平的影响.中国现代应用药学杂志,2003,20(2):101-102.
[25]高健,徐先祥,徐先俊,等.黄芪总皂苷抗血栓形成作用实验研究.中成药,2002,24(2):116.
[26]余静,郭雪娅,赵锋,等.黄芪注射液对高血压病患者血浆P选择素、血栓素B_2及6-Keto-PGF_(1a)的影响.《临床荟萃》 2005,20(13):721-723.
[27]白如君,褚伟,徐洁,等.丹参提取液对糖尿病大鼠血浆ET、TXB_2、6-Keto-PGF_(1a)的影响.湖北中医学院学报,2006,8(3):11-12.
[28]刘久波,陈林,朱少铭,等.丹参注射液对急性肾衰竭兔心肌缺血的保护作用。医药导报,2005,24(11):997-1000.
[29]王梅.丹参对重症急性胰腺炎并发肾功能损害的保护作用.临床药学,2004,13(10):65-66.
[30]何淑虹.丹参对花生四烯酸及血脂调节的大鼠实验研究.浙江中西医结合杂志,2005,15(12):749-750.
[31]肖志华,王家陇.丹参对急性肝损害大鼠体内血栓素、前列环素的作用.同济医科大学学报,1997,26(6);450-452.
[32]张建新,程国祚,李龙,等.丹参对大鼠急性坏死性胰腺炎并发肺损伤的影响.中华急诊医学杂志,2002,11(2):101-104.
[33]彭从健,钟培金,刘和强,等.刺五加注射液对急性脑梗死患者血栓素和前列环素的影响.中国中医急症,2006,15(7):732-733.
[34]睢大员,曲绍春,于小风,等.刺五加叶皂苷对大鼠心肌缺血再灌注损伤的保护作用.中国中药杂志,2004,29(1):71-74.