Caspase-1及其激活的细胞因子IL-18在大鼠急性脊髓损伤后的表达及意义
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摘要
目的半胱氨酸天冬氨酸特异性蛋白酶-1(caspase-1)及其激活的细胞因子与许多疾病的炎症反应和细胞凋亡有关,但与脊髓继发性损伤的关系目前尚不清楚。该研究旨在探讨caspase-1及其激活的细胞因子白细胞介素-18(Interleukin-18, IL-18)在大鼠急性脊髓损伤(spinal cord injury,SCI)后的表达及意义。
方法成年雄性Sprague- Dawley(SD)大鼠48只随机分为对照组(A组)和脊髓损伤组(B组),每组24只,A、B组各再分4个亚组。B组应用改良的Allen’s重物打击法建立大鼠急性脊髓损伤模型,A组只做T9全椎板切除术。分别于术后6h、1d、3d、7d处死各组大鼠,以损伤部位为中心取材,HE染色观察脊髓组织病理变化,免疫组化染色检测caspase-1、IL-18的表达情况。
结果对照组大鼠脊髓组织中少量细胞caspase-1、IL-18表达阳性。脊髓损伤后6h,损伤组caspase-1、IL-18的表达高于对照组,1d继续增高,3d表达最多,7d略有降低,但仍高于对照组。脊髓损伤组各时间点脊髓组织中caspase-1和IL-18表达阳性的细胞数较对照组显著增高(P<0.01)。
结论正常大鼠脊髓组织内存在少量caspase-1、IL-18的表达。大鼠脊髓损伤后,caspase-1和IL-18的表达迅速增强。caspase-1和IL-18的表达增强表明其可能参与了脊髓继发性损伤,并可能是损伤性因素之一。
Objective The expressions of caspase-1 and cytokines activated by caspase-1 are associated with the pathophysiology of many diseases for its proinflammatory and proapototic peculiarity. However,its relationship to spinal cord injury has not yet been identified. This study aimed to investigate the roles of caspase-1 and interleukin-18 activated by caspase-1 in the rat acute spinal cord injury (SCI).
Method 48 adult Sprague-Dawley (SD) male rats were randomly divided into two groups: The control group (group A) and spinal cord injury group(group B),each group consisted of 24 rats, group A and group B were randomly divided into four small groups.The rats of group B used modified Allen’s method established model of SCI, the rats of group A just underwent a T9 laminectomy without injury. The rats were sacrificed at 6h, 1d, 3d and 7d respectively after injury, the lesion areas of the spinal cord were dissected for morphological studies by hematoxylin and eosin staining, the expressions of caspase-1 and Il-18 were detected using immunohistochemistry method.
Result The low expressions of caspase-1 and IL-18 were observed in the control group. The expressions of caspase-1 and IL-18 in the spinal cord injury group were higher than those in control group at 6h, it go on increased at 1d, peaked at 3d, decreased at 7d. The levels of caspase-1 and IL-18 in the spinal cord injury group were obviously higher than those in the control group at 6h, and at 1, 3, 7days after injury (P<0.01).
Conclusion The low expressions of caspase-1 and IL-18 can be observed in the non-injury spinal cord. The expressions of caspase-1 and IL-18 increased quickly after SCI.The increased expressions of caspase-1 and IL-18 may play important roles in the pathogenesis of SCI.
方法成年雄性Sprague- Dawley(SD)大鼠48只随机分为对照组(A组)和脊髓损伤组(B组),每组24只,A、B组各再分4个亚组。B组应用改良的Allen’s重物打击法建立大鼠急性脊髓损伤模型,A组只做T9全椎板切除术。分别于术后6h、1d、3d、7d处死各组大鼠,以损伤部位为中心取材,HE染色观察脊髓组织病理变化,免疫组化染色检测caspase-1、IL-18的表达情况。
结果对照组大鼠脊髓组织中少量细胞caspase-1、IL-18表达阳性。脊髓损伤后6h,损伤组caspase-1、IL-18的表达高于对照组,1d继续增高,3d表达最多,7d略有降低,但仍高于对照组。脊髓损伤组各时间点脊髓组织中caspase-1和IL-18表达阳性的细胞数较对照组显著增高(P<0.01)。
结论正常大鼠脊髓组织内存在少量caspase-1、IL-18的表达。大鼠脊髓损伤后,caspase-1和IL-18的表达迅速增强。caspase-1和IL-18的表达增强表明其可能参与了脊髓继发性损伤,并可能是损伤性因素之一。
Objective The expressions of caspase-1 and cytokines activated by caspase-1 are associated with the pathophysiology of many diseases for its proinflammatory and proapototic peculiarity. However,its relationship to spinal cord injury has not yet been identified. This study aimed to investigate the roles of caspase-1 and interleukin-18 activated by caspase-1 in the rat acute spinal cord injury (SCI).
Method 48 adult Sprague-Dawley (SD) male rats were randomly divided into two groups: The control group (group A) and spinal cord injury group(group B),each group consisted of 24 rats, group A and group B were randomly divided into four small groups.The rats of group B used modified Allen’s method established model of SCI, the rats of group A just underwent a T9 laminectomy without injury. The rats were sacrificed at 6h, 1d, 3d and 7d respectively after injury, the lesion areas of the spinal cord were dissected for morphological studies by hematoxylin and eosin staining, the expressions of caspase-1 and Il-18 were detected using immunohistochemistry method.
Result The low expressions of caspase-1 and IL-18 were observed in the control group. The expressions of caspase-1 and IL-18 in the spinal cord injury group were higher than those in control group at 6h, it go on increased at 1d, peaked at 3d, decreased at 7d. The levels of caspase-1 and IL-18 in the spinal cord injury group were obviously higher than those in the control group at 6h, and at 1, 3, 7days after injury (P<0.01).
Conclusion The low expressions of caspase-1 and IL-18 can be observed in the non-injury spinal cord. The expressions of caspase-1 and IL-18 increased quickly after SCI.The increased expressions of caspase-1 and IL-18 may play important roles in the pathogenesis of SCI.
引文
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