家蚕HSP基因的表达调控研究
|
摘要
家蚕(Bombyx mori)是重要的经济昆虫和模式生物。热激蛋白(heat shock protein,HSP)是在生物界广泛存在的蛋白质,不仅在应激条件下高表达,在正常情况下的组织中也广泛存在。蜕皮激素属于类固醇激素,能促进核酸和蛋白质的合成,影响糖类、脂类的代谢,影响中枢神经系统并参与免疫调节。芸香苷是食物中常见的类黄酮物质,属黄酮醇类,有广泛的生物学活性,能抑制细胞增殖和诱导细胞凋亡。我们从NCBI上获得了家蚕10种热激蛋白基因和蛋白序列,用生物信息学的方法对BmHSP进行分析,并采用双跟踪标定实时荧光定量RT-PCR (dual-spike-in qPCR)方法,对家蚕BmHSP的基因转录水平进行了定量研究,主要研究结果如下:
1、热激蛋白的生信息学分析
用生物信息学方法对热激蛋白进行分析,结果表明:Bmhsp20.4、Bmhsp20.8和Bmhsp19.9的亲缘关系最近,小分子热激蛋白的同源性较高, Bmhsp21.4在进化中处于独立的分支。对小分子热激蛋白二级结构域的分析比较认为,家蚕的7种小分子热激蛋白序列有较高的保守性,而且都相似的α螺旋区域。对启动子区域的转录因子结合位点预测发现,结合位点存在差异,又有相似性,推测与基因的功能相关。
2、蜕皮激素对BmHSP的诱导表达定量分析。
测定了家蚕幼虫经蜕皮激素诱导后,热激蛋白在中肠、脂肪体和马氏管中的表达水平。结果表明:Bmhsp70在各个组织中检测不到表达。在中肠中, Bmhsp21.4的表达水平在刺激后显著升高,表明该基因在中肠中起重要作用;Bmhsp25.4在刺激后的脂肪体中持续高表达,表明该基因在脂肪体中有重要作用。不同的基因在不同的组织中,表达水平表现出时序的差异。
3、芸香苷诱导家蚕HSP基因的表达变化
用实时荧光定量RT-PCR的方法测定了芸香苷刺激家蚕后热激蛋白在中肠、脂肪体和马氏管中的表达水平。结果表明:Bmhsp70在各个组织中检测不到表达;不同的组织中基因的转录水平差异很大,并且不同的基因呈现出不同的表达模式,且基因表达时序的不同与启动子结合位点的差异可能有一定的相关性,Bmhsp19.9、Bmhsp20.1、Bmhsp25.4均有较高的转录水平和相似的转录的模式,推测他们在家蚕对芸香苷的代谢中是有着非常重要的作用。
本文对家蚕10种热激蛋白基因和蛋白序列进行了生物信息学分析,并通过定量的方法检测了家蚕热激蛋白在蜕皮激素和芸香苷的诱导的表达水平,发现在不同的刺激下,不同组织、不同时间内,不同的基因有不同的表达模式,且基因表达时序的差异可能与转录因子的结合有关。为热激蛋白在生物体内的作用提供参考,为后续的研究提供基础和依据。
Silkworm is an important economic insects and model organisms. Heat shock protein (heat shock protein, HSP) exists in biology widely, not only in the high expression under stress, but also widespread under normal circumstances. Ecdysone can promote the synthesis of nucleic acids and proteins, affecting carbohydrate lipid metabolism, affecting the central nervous system and immune regulation. Rutin is a common flavonol of flavonoids can inhibit cell proliferation and induce apoptosis. In this paper, we analysised BmHSPs with the method of bioinformatics, investigated the transcription levels of BmHSP genes in Bombyx mori with dual-spike-in qPCR method. The main results are as follows:
1 bioinformatics analysis of heat shock proteins
Using bioinformatics methods analyzed heat shock proteins, the results showed that: Bmhsp20.4, Bmhsp20.8 and Bmhsp19.9 have the closest relationship, small heat shock proteins were highly homologous, and Bmhsp21.4 was an independent branch in evolution. The secondary structure analysis of small heat shock proteins revealed that the sequences of 7 silkworm small heat shock proteins were quite conservative containing the similarα-helix region. Predicting the binding sites of transcription fators on promoters of BmHSPs revealed that the binding sites were different but had similarities, suggesting that maybe they were associated with fuctions,
2 the expressions of BmHSPs in tisusses inducted by ecdysone
Expressions were determined in the midgut, fat body and malpighian tubules after induction by ecdysone. The results showed that: we could not detect the expression of Bmhsp70. In midgut, expression of Bmhsp21.4 increased at 2h after induction implied its importance. In fat body, transcription of Bmhsp25.4 kept in a high level. The expressions of genes were different in tusseus and timing,
3 the expressions of BmHSPs in tisusses inducted by rutin
We detected the expression of each gene in tissues with qRT-PCR. The results showed that: the expression of Bmhsp70 was not detected; transcriptions were very different of genes, and different genes showed different expression patterns.We inferred the promoter binding sites may have some correlation with gene expressions.
In this study we analysised ten heat shock protein genes with bioinformatics method, illustrate the expression levels of BmHSPs in different organizations inducted by ecdysone and rutin, we found that different gene has different expression modols after different induction in tissues and time, and the differences in gene expression may be related to the binding positions of transcription factors.The results can provide data for studying the function of BmHSPs in vivo and for subsequent research.
1、热激蛋白的生信息学分析
用生物信息学方法对热激蛋白进行分析,结果表明:Bmhsp20.4、Bmhsp20.8和Bmhsp19.9的亲缘关系最近,小分子热激蛋白的同源性较高, Bmhsp21.4在进化中处于独立的分支。对小分子热激蛋白二级结构域的分析比较认为,家蚕的7种小分子热激蛋白序列有较高的保守性,而且都相似的α螺旋区域。对启动子区域的转录因子结合位点预测发现,结合位点存在差异,又有相似性,推测与基因的功能相关。
2、蜕皮激素对BmHSP的诱导表达定量分析。
测定了家蚕幼虫经蜕皮激素诱导后,热激蛋白在中肠、脂肪体和马氏管中的表达水平。结果表明:Bmhsp70在各个组织中检测不到表达。在中肠中, Bmhsp21.4的表达水平在刺激后显著升高,表明该基因在中肠中起重要作用;Bmhsp25.4在刺激后的脂肪体中持续高表达,表明该基因在脂肪体中有重要作用。不同的基因在不同的组织中,表达水平表现出时序的差异。
3、芸香苷诱导家蚕HSP基因的表达变化
用实时荧光定量RT-PCR的方法测定了芸香苷刺激家蚕后热激蛋白在中肠、脂肪体和马氏管中的表达水平。结果表明:Bmhsp70在各个组织中检测不到表达;不同的组织中基因的转录水平差异很大,并且不同的基因呈现出不同的表达模式,且基因表达时序的不同与启动子结合位点的差异可能有一定的相关性,Bmhsp19.9、Bmhsp20.1、Bmhsp25.4均有较高的转录水平和相似的转录的模式,推测他们在家蚕对芸香苷的代谢中是有着非常重要的作用。
本文对家蚕10种热激蛋白基因和蛋白序列进行了生物信息学分析,并通过定量的方法检测了家蚕热激蛋白在蜕皮激素和芸香苷的诱导的表达水平,发现在不同的刺激下,不同组织、不同时间内,不同的基因有不同的表达模式,且基因表达时序的差异可能与转录因子的结合有关。为热激蛋白在生物体内的作用提供参考,为后续的研究提供基础和依据。
Silkworm is an important economic insects and model organisms. Heat shock protein (heat shock protein, HSP) exists in biology widely, not only in the high expression under stress, but also widespread under normal circumstances. Ecdysone can promote the synthesis of nucleic acids and proteins, affecting carbohydrate lipid metabolism, affecting the central nervous system and immune regulation. Rutin is a common flavonol of flavonoids can inhibit cell proliferation and induce apoptosis. In this paper, we analysised BmHSPs with the method of bioinformatics, investigated the transcription levels of BmHSP genes in Bombyx mori with dual-spike-in qPCR method. The main results are as follows:
1 bioinformatics analysis of heat shock proteins
Using bioinformatics methods analyzed heat shock proteins, the results showed that: Bmhsp20.4, Bmhsp20.8 and Bmhsp19.9 have the closest relationship, small heat shock proteins were highly homologous, and Bmhsp21.4 was an independent branch in evolution. The secondary structure analysis of small heat shock proteins revealed that the sequences of 7 silkworm small heat shock proteins were quite conservative containing the similarα-helix region. Predicting the binding sites of transcription fators on promoters of BmHSPs revealed that the binding sites were different but had similarities, suggesting that maybe they were associated with fuctions,
2 the expressions of BmHSPs in tisusses inducted by ecdysone
Expressions were determined in the midgut, fat body and malpighian tubules after induction by ecdysone. The results showed that: we could not detect the expression of Bmhsp70. In midgut, expression of Bmhsp21.4 increased at 2h after induction implied its importance. In fat body, transcription of Bmhsp25.4 kept in a high level. The expressions of genes were different in tusseus and timing,
3 the expressions of BmHSPs in tisusses inducted by rutin
We detected the expression of each gene in tissues with qRT-PCR. The results showed that: the expression of Bmhsp70 was not detected; transcriptions were very different of genes, and different genes showed different expression patterns.We inferred the promoter binding sites may have some correlation with gene expressions.
In this study we analysised ten heat shock protein genes with bioinformatics method, illustrate the expression levels of BmHSPs in different organizations inducted by ecdysone and rutin, we found that different gene has different expression modols after different induction in tissues and time, and the differences in gene expression may be related to the binding positions of transcription factors.The results can provide data for studying the function of BmHSPs in vivo and for subsequent research.
引文
[1]中国科学院北京基因组研究所.基因组学研究领域的两个重要成就-家蚕基因组框架图和家鸡基因组多态性图谱的绘制[J].中国科学院知识创新工程重大项目, 2007, 22(5): 421-422
[2] Ritossa, F. A new puffing pattern induced by temperature shock and DNP in Drosophila [J]. Experientia, 1962, 18: 1-573
[3] Tisseres, A., A.K.Mitchel1. Some new proteins induced by temperature shock in Drosophila [J]. J.Mo1.Biol, 1974, 84:389-398
[4] Feder M, et al. Heat shock protein, molecular chaperones, and the stress response: evolutionary and ecological physiology [J]. Amu.Reu.Phys, 1999, 61: 243-282
[5] Guttmans D, Glover C V, Allis C D, et al. Heat shock, deciliation and release from anoxia induce the synthesis of the same polypeptides in starved T pyriformis [J]. Cell, 1980, 22: 299-307
[6] Yang XM, Baxter GF, Heads RJ, et al. Infarct limitation of the stress Response [J]. J Clin Invet, 1995, 95 (1)
[7] Barbe M F, Tytell M, Gower D J,et al. Hyperthermia protects against lignt damage in the rat retina [J]. Science, 1988, 241: 1817-1820
[8] Levinson W, Oppermann H, Jackson J. Transitionseries matals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cells [J]. Biochimica et Biophysica Acta, 1980, 606(1):170-180
[9] Wditzel G, Pilatus U, Rending L. Similar dose response of heat shock protein synthesis and intracellular pH chang in yeast [J]. Experimental Cell Research, 1985, 159: 252-256
[10] Kelley P M, Schlesinger M J. The effects of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts [J]. Cell, 1978, 15(4): 1277-1286
[11] Pouyssegur J, Shiu P C, Psatan I. Induction of two transformation-sensitive membrane polypeptides in normal fibroblasts by a block in glycoprotein synthesis or glucose deprivation [J]. Cell, 1977, 11(4): 941-947
[12] Morimoto, RI. Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators [J]. Genes Dev, 1998, 12: 3788-3796
[13] Carper SW, Duffy JJ, Gerner EW. Heat shock proteins in thermo tolerance and other cellular physiological processes [J]. Cancer Research, 1987, 47(5):249-5 255
[14]张莉,刘吉平.热激蛋白研究进展[J].广东蚕业, 2006, 40(2):39-42
[15]陈亚琼.热激蛋白与生物环境适应及进化的关系[J].自然科学进展, 2006, 16(9):1066-1073
[16]Sugiyama Y, Suzuki A, Kishikawa M, et al. Muscle develops a specific form of small heat shockprotein complex composed of MKBP/HSPB2 and HSPB3 during myogenic differentiation [J]. J Biol Chem, 2000, 275(2): 1095-1104
[17] Yuki Sugiyama Muscle Develops a Specific Form of Small Heat Shock Protein Complex Composed of MKBP/HSPB2 and HSPB3 during Myogenic Differentiation [J]. J Biol Chem, 2000, 275(2): 1095-1104
[18] Norimine,J.,J.Mosqueda,G.H. Palmer,et al. Conservation of Babesia bovis Small heat shock protein(Hsp20) among strains and definition of T helper cell eprtopes recognized by cattle with diverse major hisocompatibility complex class II haplotypes [J].Infect.Immun, 2004, 72:1906-1106
[19] Chowdary, T. K., R. Bakthisaran, et al. Mammalian Hsp22 is a heat-inducible small heat shock protein with chaperone-like activbity [J]. Biochen.J.Pt, 2004
[20]朱玉贤,李毅.现代分子生物学(第2版).北京:高等教育出版社, 2002.7: 281-28
[21] Morimoto RI, Tissieres A, Georgopoulos C. Regulation of heat shock gene transcription by a family of heat shock factors. In: Morimoto RI, Tissieres A, Georgopoulos C. (Eds), the Biology of Heat Shock Proteins and Molecular Chaperones [J]. Cold SpringHarbor Laboratory Press, NY. 1994: 335
[22] Horwttz J.α-Crystaline can function as a molecular chaperone [J]. Proc Natl Acad Sci USA, 1992, 89:10449-10453
[23]Weitzel G, Pilatus U, Rensing L. Similar dose response of heat shock protein synthesis and intracellular pH change in yeast [J]. Exp Cell Res, 1985, 159(1): 252-256
[24]Sciandra J J, Subjeck J R. The effect of glucose on protein synthesis and thermosensitivity in Chinese hamster ovary cells [J]. J Biol Chem, 1983, 258(20): 12091-12093
[25] Boston R S, Viitanen P V, Vierling E. Molecular chaperones and protein folding in plants [J]. Plant Mol Biol, 1996, 32(11-2): 191-222
[26]王建义,慈忠玲.热激蛋白的研究进展[J].山西林业科技, 2008,3(1):27-32.
[27]云标,黄涛,舒念红,等.热激蛋白的分子生物学研究进展[J].植物学通报, 1995, 12(1):1 251
[28] Kimpel J A, Nagao R T, Goekjian V, et a1, Regulation of the heat shock response in soybean seedlings [J]. Plant Physiol, 1990, 94: 988-995
[29]Vierling E, Annu R. Plant Physiol [J]. Plant Mol Biol, 1991, 42: 579-620
[30] Waters ER, Lee GJ, Vierling E. Evolution, structure and function of the small heat shock proteins in plants [J]. J Exp Bot, 1996.47:325～338
[31] Meyer Y, Chartier Y. Long Lived and Short Lived Heat shock Proteins in Tobacco Mesophy 1 1 Proto-plasts [J]. Plant Physiol, 1983, 72: 26-32
[32] Necehi A, Pogna N E, Mapelli S. Early and Late Heat Shock Proteins in Wheats and Other Cereal Species [J]. Plant Physiology, 1987, 84: 1378-1384
[33] Williams K J, Landgraf B E, Whiting N L, et al. Correlation between the induction of heat shock protein 70 and enhanced viral reactivation in mammalian cells treated with ultraviolet light and heatshock [J]. Cancer Res, 1989, 49 (10):2735-2742
[34] Mendez F, Kozin E, Bases R. Heat shock protein 70 stimulation of the deoxyribonucleic aced base excision repair enzyme polymerase beta [J]. Cell Stress Chaperones, 2003, 8 (2): 153-161
[35] Gimenez-Abian M I, Rozalen A E, Carballo J A, et al. Hsp90 and checkpoine dependent lengthening of the G2 phase observed in plant cells under hypoxia and cold [J]. Protoplasma, 2004, 223 (24): 191-196
[36] de Carcer G. Heat shock protein 90 regulates the metaphase anaphase transition in a polo-like kinase-dependent manner [J]. Cancer Res, 2004, 64 (15): 5106-5112
[37] Braga Basaria M, Hardy E, Gorrfried R, et al. 17-allylamino-17-demethoxygeldanamycin activity against thyroid cancer cell lines correlates with heat shock protein 90 levels. J Clin Endocrinol Metab, 2004, 89 (6): 1982-1988
[38] Robertson J D, Datta K, Biswal S S, et al. Heat shock protein 70 antisense oligomers enhance proteasome inhibitor induced apoptosis. Biochem J, 1999, 344 (2): 477-485
[39] Walker G A, White T M, McColl G, et al. Heat shock protein accumulation is upregulated in a long-lived mutant of Caenorhabditis elegans.J Gerontol A Biol Sci Med Sci, 2001, 56 (7): 281-287
[40] Morrow G, Samson M, Michaud S, et al. Overexpression of the small mitochondrial hsp 22 extends Drosophila life span and increases to oxidative stress. Faseb J, 2004, 18 (3): 598-599
[41]王海鸿,雷仲仁.昆虫热休克蛋白的研究进展[J].中国农业科学, 2005,38 (10): 2023-2034
[42]吴学记,徐金森.热休克蛋白Hsp70 ATP-ADP转换活性及分子伴侣功能研究[J].细胞生物学,2008
[43] Beckmann R P, Mizzen L A, Welch W J. Interaction of HSP70 newly synthesized proteins: Implications for protein folding and assembly events [J]. Science, 1990, 248: 654-850
[44] Gething M J, Sambrook J. Protein folding in the cell [J]. Nature, 1992, 355 (6): 33-45
[45] Yeh C H, Chang P L, Lin W C, et al. Expression of a gene encoding a 16.9kDa heat shock protein shsp16.9, in Escherichia coli enhances thermo tolerance [J]. Proc Ncad Sci, 1997, 94:10967-10972
[46]Vierling E. The roles of heat shock proteins in plants [J]. Annu Rev Plant Physiol Plant Mol Biol, 1991, 42:579-620
[47]Dahlgaard J, Loeschcke V P, Michalak J. Induced thermo tolerance and associated expression of the heat shock protein HSP70 in adult Drosophila melanogaster [J]. Functional Ecology, 1998, 12: 786-793
[48] Li G C. Elevated levels of 70 000 dalton heat shock protein in transiently thermo tolerant Chinese hamster fibroblasts and in their stable heat resistant variants [J]. International Journal of Radiation Oncology Biology Physics, 1985, 11: 165-177
[49]王秋香,李宗芸.果蝇热激蛋白的研究进展[J].昆虫知识, 2008,45(4)679-720
[50]许竟成,蔡亚非.动物热激蛋白的研究进展[J].资源开发与市场, 2009, 25 (7) 619-622
[51] Guttman S D, Glover C V, Allis C D, et a1. Heat shock, deciliation and release from anoxia inducethe synthesis of the same polypeptides in starved T pyriformis [J]. Cell, 1980, 22: 299-307
[52] Hahn G M, Li G C. Thermotolerance and heat shock proteins in mammalian cells [J]. Radiation Research, 1982, 92: 452-457
[53] Marber M, Mestril R, Chi S H, et a1. Over expression of the rat inducible 70kDa heat stress protein in a transgenic mouse increases the resistance of the heart to ischemic injury [J]. The Journal of Clinical Investigation, 1995, 95(4): 1446-1456
[54] Weitzel G, Pilatus U, Rensing L. Similar dose response of heat shock protein synthesis and intracellular pH change in yeast [J]. Experimental Cell Research, 1985, 159: 252-256
[55] Bruemmer-Smith S, Stuber F, Schroeder. Protective functions of intracellular heat shock protein (HSP) 70 expression in patients with severe sepsis [J].Intensive Care Med.2001, 27:1835-1841
[56] Duverger O, Paslaru L, Morange M. HSP25 is involved in two steps of the differentiation of PAM212 keratinocytes [J]. J Biol Chem, 2004, 279 (11): 10252-10260
[57] Miyata Y. Molecular chaperone HSP90 as a novel target for cancer chemotherapy [J]. Nippon Yakurgaku Zasshi, 2003, 121(1): 33-42
[58] Bond U, Schlesinger M J. Heat shock proteins and development [J]. Adv Genet. 1987, 24: 1-29
[59] Zimmermann J L, Petri W, Meselson M. Accumulation of a specific subset of D. melanogaster heat shock mRNAs in normal development without heat shock [J]. Cell, 1983, 32: 1161-1170
[60] Wehmeyer N, Hernandez L D, Finkelstein R R, et a1. Plant Physiol. 1996.112, 747-757
[61] Prandl R, Schoffl F. Plant Mol. Biol. 1996, 31, 157-I62.
[62] Yocum G D, Joplin K H, Denlinger D L. Upregulation of a 23kDa small heat shock protein transcript during pupal diapause in the flesh fly, Sarcophaga crassipalpis [J]. Insect Biochemistry and Molecular Biology, 1998, 28, 677-682
[63] Morrison L E, Hoover H E, Thuerauf DJ, et a1. Mimicking phosphorylation of alphaB-crystallin on serine-59 is necessary and sufficient to provide maximal protection of cardiac myocytes from apoptosis [J]. Circ Res, 2003, 92(2):203-211
[64] Ehrnsperger M, Graber S, Gaestel M, et al. Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation [J]. EMBO J, 1997, 16(2):221-229
[65] Arata S, Hamaguchi S, Nose K. Inhibition of colony formation of NIH 3T3 cells by the expression of the small molecular weight heat shock protein HSP27: involvement of its phosphorylation and aggregation at the C-terminal region [J]. J Cell Physiol, 1997, 170(1):19-26
[66] Benndorf R, Hayess K, Ryazantsev S, et al. Phosphorylation and supramolecular organization of murine small heat shock protein HSP25 abolish its actin polymerization-inhibiting activity [J]. J Biol Chem, 1994, 269(32):20780-20784
[67] Preville X, Schultz H, Knauf U, et al. Analysis of the role of Hsp25 phosphorylation reveals the importance of the oligomerization state of this small heat shock protein in its protective function against TNFalpha- and hydrogen peroxide-induced cell death [J]. J Cell Biochem, 1998, 69(4):436-452
[68] Clark JI, Muchowski PJ. Small heat-shock proteins and their potential role in human disease [J]. Curr Opin Struct Biol, 2000, 20(1):52-59
[69] Fan GC, Chu G, Mitton B, et al. Small heat-shock protein Hsp20 phosphorylation inhibits beta-agonist-induced cardiac apoptosis [J]. Circ Res, 2004, 94(11):1474-1482
[70]万永奇,谢维.生命科学与人类疾病研究的重要模型-果蝇[J].生命科学, 2006, 18(5): 425-429
[71] Ciocca,DR., Fuqua,SA, Lock Lin S,et al. Response of human breast cancer cells to heat shock and chemotherapeutic drugs [J]. Cancer Res. 1992, 52:3648-3654
[72] Susek, RE, Lindquist SL. HSP26 of saccharomyces cerevisiac is related to the super family of small heat shock proteins bur is without a demonstrable function [J]. Mol.Cell. 1989, 9 (11):5265-5272
[73] Daohai Zhang, Thomas C. Putti. Over-expression of ERp29 attenuates doxorubicin-induced cell apoptosis through up-regulation of Hsp27 in breast cancer cells [J]. Experimental Cell Research, 2010, 316(20): 3522-3531
[74] Favet N, Duverger O, Loones M T, et a1. OverexDression of murine small heat shock protein HSP25 interferes with chondrocyte differentiation and decreases cell adhesion [J]. Cell Death Differ, 2001, 8(6):603-613
[75]秦焱,王慧,昌增益.线虫中的小分子热休克蛋白HSP12.1具有类分子伴侣活性[J].生物化学与生物物理进展,2007,34(6):620-624
[76] Haass C, Klein U, Kloetzel P M. Developmental expression of Drosophila melanogaster small heat-shock proteins [J]. J Cell Sci, 1990, 96 (Pt 3): 413-418
[77]王佳颖,张景霞,刘箭,等.组成型表达线粒体小分子热激蛋白提高番茄抗冷性.山东师范大学(自然科学版). 2008, 23 (4): 100-102.
[78]肖成峰,陈胜,高雅娟. HSP71在抗癌药物足草乙甙致成人肝细胞凋亡中的作用[J].中华劳动卫生职业病杂志, 2000, 18(2):78-81
[79]宋松.幼苗期小麦热激蛋白的诱导研究[J].安徽农业科学, 2010, 38(2):595-596
[80]张敏,李建成,张青文,等.热激蛋白90与中红侧沟茧蜂的发育关系[J].昆虫知识, 2009, 46 (2): 284-289
[81]童田雨,冯向飞.热激蛋白70在兔动脉粥样硬化易损斑块内的高表达[J].中国临床保健杂志, 2009, 12 (1): 44-48
[82]张作法,时连根.桑枝芸香苷对硫代乙酰胺引起的小鼠急性肝损伤的保护作用[J].中国蚕学会第五届青年学术研讨会论文集,苏州, 2007. 9: 33 - 34
[83]方玲芬.曲克芦丁注射液无菌检查法方法学研究[J].贵阳医学院学报, 2006, 31 (6) : 590 - 592
[84]武征,汪琳,张建华,等.芸香苷对实验性急性胰腺炎胰外多器官损伤的保护作用及机制[J].中国中医基础医学杂志, 2007, 13 (3) : 211 - 215
[85]李家富,何涛,李刚,等.醋柳黄酮单体对高密度脂蛋白氧化修饰的影响[J].现代中西医结合杂志, 2004, 13(24) : 3234 - 3235
[86]赵国屏.生物信息学[M].北京,科学出版社: 2000
[87]王魏杰.生命科学领域的前沿科学:生物信息学[J].河北理工学院学报, 2004, 26(1): 126-128
[88]张成岗,贺福初.生物信息学方法与实践[M],北京,科学出版社, 2002
[89]李斌,夏庆友,鲁成,等.低分子量热激蛋白Bmhsp19.9基因的表达[J].农业生物技术学报, 2005, 13 (2): 195-201
[90]谢敏,贡成良,薛仁宇,等.家蚕hsp20.4启动子克隆及其驱动表达产物EGT对家蚕蛹体发育的影响[J].昆虫学报, 2009, 52 (3) : 246-253
[91]李斌,夏庆友,鲁成.家蚕肾脏形卵(ki)的荧光差异显示研究[J].农业生物技术学报, 2002, 10 (3): 262-266
[92]孙卫忠,李斌,鲁成,等.家蚕HSP20.8基因体外表达及荧光原位杂交研究[J].蚕业科学, 2007, 33 (1):14-19
[93]李斌,夏庆友,鲁成,等.低分子量热激蛋白的进化研究[J].蚕业科学, 2004, 30 (3): 260-265
[94]张静.家蚕热休克蛋白Bmhsp21.4基因的研究[D].浙江理工大学, 2010
[95] Qing Sheng, Jiayin Xia, Yaozhou Zhang, et al. Cloning, Expression, and Cell Localization of a Novel Small Heat Shock Protein Gene: BmHSP25.4 [J]. Appl Biochem Biotechnol, 2010, 162(5): 1297-1305
[96] Basha E, Lee GJ, Breci LA, et al. The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions [J]. J Biol Chem, 2004, 279(9):7566-7575.
[97] Friedrich KL, Giese KC, Buan NR, et al. Interactions between small heat shock protein subunits and substrate in small heat shock protein-substrate complexes [J]. J Biol Chem, 2004, 279(2):1080-1089.
[98] Haslbeck M, Braun N, Stromer T, et al. Hsp42 is the general small heat shock protein in the cytosol of Saccharomyces cerevisiae [J]. EMBO J, 2004, 23(3):638-649
[99] Qazi KR, et a1. Vaccine, 2005, 23(9):1114-1125
[100]王荫长主编.昆虫生物化学[M].北京:中国农业出版社. 2001.
[101] Adnan S, Susan L, David W. Expression of small heat shock proteins at low temperatures [J].Plant Physiologists, 1998, 117(2):651-658
[102] Leshem Y, Kuiper P. Is there a GAS (general adaption syndrome) response to barious types of environmental stress [J].Biol Plant, 1996, 38:118
[103]许仁林,师素云,易琼华.热激处理与水稻雄性不育系育性转变关系的初步研究[J].武汉植物学研究,1992,10(4):297-304
[104]陈建南,傅鸿仪,路子显,等.温度、热激蛋白与高粱育性的变化[J].遗传学报, 1998, 25(4): 356-361
[105] Sanders BM. Stress proteins in aquatic organisms: an environmental perspective [J]. Crit Rev Toxicol, 1993, 23: 49-75.
[106] Huggett R. Enzyme and protein synthesis as indicators of contaminant exposure and effect [J]. Florida: Lewis Boca Raton, 1 992:235-335
[107]培军,熊先哲,杨桂芬,等.动物生物标志物在土壤污染物生态学研究中的应用[J],应用生态学报, 2003, 14(12): 2347-2350
[108] Staempfli C, Beeker-van Slooten K, Tarradellas J. HSP70 instability and induction by a pesticide in Folsomia Candida [J]. Biomarker, 2002, 7(1): 68-79
[109] Queitsch Cetal. HSP90 as a capacitor of phenotypic variation [J]. Nature, 2002, 4 (17): 618-624.
[110]邓家术,段彬江,刘中来.植物热激蛋白的研究进展及应用[J].生命的化学, 2003, 23(3): 226-228.
[111]Laszlo O Jr, Insug O, Mark E R, et al. Interaction between Heat Shock Proteins and Antimicrobial Peptides [J]. Biochemistry 2000, 39, 14150-14159
[112] Lima KM, Santos R R, Brandao I T, et a1. Efficacy of DNA HSP65 vaccination for tuberculosis varies with method of DNA introduction in vivo [J]. Vaccine, 2003, 22(1):49-56.
[113] Manjili M H, Wang X Y, Park J, et a1. Immunotherapy of cancer using heat shock proteins [J]. Front Biosci, 2002, 7:43-52
[114] Higuchi R, Fockler C, Dollinger G, et al. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions [J]. Biotechnology (N Y), 1993, 11(9): 1026-1030
[115]J Sambrook, E F Fritsch, T Maniatis. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989
[116] Yi Zhang, Zhenguo Wei, YuanYuan Li, et al. Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy [J].Analytical Biochemistry, 2009, 394: 202-208
[117]黄惠芳,马飞.热激蛋白的分子进化研究[J].厦门大学学报(自然科学版), 2004, 43: 166-170
[118]李斌.家蚕分子连锁图谱的构建和低分子量热激蛋白基因的克隆与特异表达[D].西南农业大学, 2001
[119] Li Z W, Xiang Z H, Zhang Z, et al. The small heat shock protein (sHSP) genes in the silkworm, Bombyx mori, and comparative analysis with other insect sHSP genes. BMC Evolutionary Biology, 2009, 9:215
[120] Otaka T. Chromosomal action of ecdysone [J]. Nature, 1980, 285(12):435
[121] Catalan RE, Martines A M, Aragones MD. In vitro effect of ecdysterone on protein kinase activity [J]. Comp Biochen Physiol.1982, 71B (2):301
[122] Takei M, Endo K,Nishimmoto N, et al. Effect of ecdysterone on histamine release fro rat peritoneal mast cells [J]. Pharm Sci, 1991,80 (4):309
[123] Catalan RE, Aragones MD, Godoy JE, et al. Ecdysterone induces acetylcholinesterase in mammalian brain [J]. Comp Biochel Physiol, 1984, 78 C (1):193
[124] Manglesdorf D J, Thummel C, Beato M, et al. The nuclear receptor superfamily: the second decade [J]. Cell, 1995, 83: 835 - 839
[125]Henrich V C, Rybczynski R, Gilbert L I. Peptide hormones, steroid hormones, and puffs: Mechanisms and models in insect development [J]. Vitamins and Hormones, 1999, 55: 73-125.
[126]Gilbert LI, Rybczynski R, Warren JT. Control and biochemical nature of the ecdysteroidogenic pathway [J]. Annual Review of Entomology, 2002, 47: 883 - 916.
[127] Gu SH, Chow YS. Analysis of ecdysteroidogenic activity of the prothoracic glands during the last larval instar of the silkworm, Bombyx mori [J]. Arch. Insect Biochem. Physiol, 2005a, 58 (1): 17-26
[128] Gu SH, Chow YS. Temporal changes in DNA synthesis of prothoracic gland cells during larval development and their correlation with ecdysteroidogenic activity in the silkworm, Bombyx mori [J]. J Exp Zoolog A Comp Exp Biol, 2005b, 303 (4): 249-258
[139]郭恩恩,李胜,曹阳, 2008.昆虫蜕皮的激素调控网络[J].蚕业科学, 34(2) : 370-374
[130]路爱成,卫正国,李兵,沈卫德, 2009.野桑蚕不同组织细胞色素P450 CYP305B1V1基因的诱导表达特征研究[J].安徽农业科学. 37(12): 5400-5402
[131]孔岳樵.蚕桑的蜕皮激素[J].蚕桑茶叶通讯,1994(1):36-39
[132]徐世清,徐俊良.蜕皮激素对桑蚕的影响及应用前景[J].江苏蚕业, 1995, 2: 1-4
[133]赵国栋,卫正国,沈卫德,等.家蚕谷胱甘肽-S-转移酶基因(BmGSTd1)的诱导表达定量分析[J].蚕业科学, 2010, 36(1):46-51
[134]卫正国.家蚕细胞色素P450基因及其定量方法的研究[D].苏州:苏州大学, 2009
[135]de Miguel N, Braun N, Bepperling A, et al. Structural and functional diversity in the family of small heat shock proteins from the parasite Toxoplasma gondii [J]. Biochim Biophys Acta, 2009, 1793(11): 1738-1748
[136]Carrasco R, Almoguera C, Jordano J. A plant small heat shock protein gene expressed during zygotic embryogenesis but noninducible by heat stress [J]. J Biol Chem, 1997, 272(43): 27470-27475
[137]王琛柱.植物抗虫性的化学基础[J].植物保护, 1993, 19(6):39-41
[2] Ritossa, F. A new puffing pattern induced by temperature shock and DNP in Drosophila [J]. Experientia, 1962, 18: 1-573
[3] Tisseres, A., A.K.Mitchel1. Some new proteins induced by temperature shock in Drosophila [J]. J.Mo1.Biol, 1974, 84:389-398
[4] Feder M, et al. Heat shock protein, molecular chaperones, and the stress response: evolutionary and ecological physiology [J]. Amu.Reu.Phys, 1999, 61: 243-282
[5] Guttmans D, Glover C V, Allis C D, et al. Heat shock, deciliation and release from anoxia induce the synthesis of the same polypeptides in starved T pyriformis [J]. Cell, 1980, 22: 299-307
[6] Yang XM, Baxter GF, Heads RJ, et al. Infarct limitation of the stress Response [J]. J Clin Invet, 1995, 95 (1)
[7] Barbe M F, Tytell M, Gower D J,et al. Hyperthermia protects against lignt damage in the rat retina [J]. Science, 1988, 241: 1817-1820
[8] Levinson W, Oppermann H, Jackson J. Transitionseries matals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cells [J]. Biochimica et Biophysica Acta, 1980, 606(1):170-180
[9] Wditzel G, Pilatus U, Rending L. Similar dose response of heat shock protein synthesis and intracellular pH chang in yeast [J]. Experimental Cell Research, 1985, 159: 252-256
[10] Kelley P M, Schlesinger M J. The effects of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts [J]. Cell, 1978, 15(4): 1277-1286
[11] Pouyssegur J, Shiu P C, Psatan I. Induction of two transformation-sensitive membrane polypeptides in normal fibroblasts by a block in glycoprotein synthesis or glucose deprivation [J]. Cell, 1977, 11(4): 941-947
[12] Morimoto, RI. Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators [J]. Genes Dev, 1998, 12: 3788-3796
[13] Carper SW, Duffy JJ, Gerner EW. Heat shock proteins in thermo tolerance and other cellular physiological processes [J]. Cancer Research, 1987, 47(5):249-5 255
[14]张莉,刘吉平.热激蛋白研究进展[J].广东蚕业, 2006, 40(2):39-42
[15]陈亚琼.热激蛋白与生物环境适应及进化的关系[J].自然科学进展, 2006, 16(9):1066-1073
[16]Sugiyama Y, Suzuki A, Kishikawa M, et al. Muscle develops a specific form of small heat shockprotein complex composed of MKBP/HSPB2 and HSPB3 during myogenic differentiation [J]. J Biol Chem, 2000, 275(2): 1095-1104
[17] Yuki Sugiyama Muscle Develops a Specific Form of Small Heat Shock Protein Complex Composed of MKBP/HSPB2 and HSPB3 during Myogenic Differentiation [J]. J Biol Chem, 2000, 275(2): 1095-1104
[18] Norimine,J.,J.Mosqueda,G.H. Palmer,et al. Conservation of Babesia bovis Small heat shock protein(Hsp20) among strains and definition of T helper cell eprtopes recognized by cattle with diverse major hisocompatibility complex class II haplotypes [J].Infect.Immun, 2004, 72:1906-1106
[19] Chowdary, T. K., R. Bakthisaran, et al. Mammalian Hsp22 is a heat-inducible small heat shock protein with chaperone-like activbity [J]. Biochen.J.Pt, 2004
[20]朱玉贤,李毅.现代分子生物学(第2版).北京:高等教育出版社, 2002.7: 281-28
[21] Morimoto RI, Tissieres A, Georgopoulos C. Regulation of heat shock gene transcription by a family of heat shock factors. In: Morimoto RI, Tissieres A, Georgopoulos C. (Eds), the Biology of Heat Shock Proteins and Molecular Chaperones [J]. Cold SpringHarbor Laboratory Press, NY. 1994: 335
[22] Horwttz J.α-Crystaline can function as a molecular chaperone [J]. Proc Natl Acad Sci USA, 1992, 89:10449-10453
[23]Weitzel G, Pilatus U, Rensing L. Similar dose response of heat shock protein synthesis and intracellular pH change in yeast [J]. Exp Cell Res, 1985, 159(1): 252-256
[24]Sciandra J J, Subjeck J R. The effect of glucose on protein synthesis and thermosensitivity in Chinese hamster ovary cells [J]. J Biol Chem, 1983, 258(20): 12091-12093
[25] Boston R S, Viitanen P V, Vierling E. Molecular chaperones and protein folding in plants [J]. Plant Mol Biol, 1996, 32(11-2): 191-222
[26]王建义,慈忠玲.热激蛋白的研究进展[J].山西林业科技, 2008,3(1):27-32.
[27]云标,黄涛,舒念红,等.热激蛋白的分子生物学研究进展[J].植物学通报, 1995, 12(1):1 251
[28] Kimpel J A, Nagao R T, Goekjian V, et a1, Regulation of the heat shock response in soybean seedlings [J]. Plant Physiol, 1990, 94: 988-995
[29]Vierling E, Annu R. Plant Physiol [J]. Plant Mol Biol, 1991, 42: 579-620
[30] Waters ER, Lee GJ, Vierling E. Evolution, structure and function of the small heat shock proteins in plants [J]. J Exp Bot, 1996.47:325～338
[31] Meyer Y, Chartier Y. Long Lived and Short Lived Heat shock Proteins in Tobacco Mesophy 1 1 Proto-plasts [J]. Plant Physiol, 1983, 72: 26-32
[32] Necehi A, Pogna N E, Mapelli S. Early and Late Heat Shock Proteins in Wheats and Other Cereal Species [J]. Plant Physiology, 1987, 84: 1378-1384
[33] Williams K J, Landgraf B E, Whiting N L, et al. Correlation between the induction of heat shock protein 70 and enhanced viral reactivation in mammalian cells treated with ultraviolet light and heatshock [J]. Cancer Res, 1989, 49 (10):2735-2742
[34] Mendez F, Kozin E, Bases R. Heat shock protein 70 stimulation of the deoxyribonucleic aced base excision repair enzyme polymerase beta [J]. Cell Stress Chaperones, 2003, 8 (2): 153-161
[35] Gimenez-Abian M I, Rozalen A E, Carballo J A, et al. Hsp90 and checkpoine dependent lengthening of the G2 phase observed in plant cells under hypoxia and cold [J]. Protoplasma, 2004, 223 (24): 191-196
[36] de Carcer G. Heat shock protein 90 regulates the metaphase anaphase transition in a polo-like kinase-dependent manner [J]. Cancer Res, 2004, 64 (15): 5106-5112
[37] Braga Basaria M, Hardy E, Gorrfried R, et al. 17-allylamino-17-demethoxygeldanamycin activity against thyroid cancer cell lines correlates with heat shock protein 90 levels. J Clin Endocrinol Metab, 2004, 89 (6): 1982-1988
[38] Robertson J D, Datta K, Biswal S S, et al. Heat shock protein 70 antisense oligomers enhance proteasome inhibitor induced apoptosis. Biochem J, 1999, 344 (2): 477-485
[39] Walker G A, White T M, McColl G, et al. Heat shock protein accumulation is upregulated in a long-lived mutant of Caenorhabditis elegans.J Gerontol A Biol Sci Med Sci, 2001, 56 (7): 281-287
[40] Morrow G, Samson M, Michaud S, et al. Overexpression of the small mitochondrial hsp 22 extends Drosophila life span and increases to oxidative stress. Faseb J, 2004, 18 (3): 598-599
[41]王海鸿,雷仲仁.昆虫热休克蛋白的研究进展[J].中国农业科学, 2005,38 (10): 2023-2034
[42]吴学记,徐金森.热休克蛋白Hsp70 ATP-ADP转换活性及分子伴侣功能研究[J].细胞生物学,2008
[43] Beckmann R P, Mizzen L A, Welch W J. Interaction of HSP70 newly synthesized proteins: Implications for protein folding and assembly events [J]. Science, 1990, 248: 654-850
[44] Gething M J, Sambrook J. Protein folding in the cell [J]. Nature, 1992, 355 (6): 33-45
[45] Yeh C H, Chang P L, Lin W C, et al. Expression of a gene encoding a 16.9kDa heat shock protein shsp16.9, in Escherichia coli enhances thermo tolerance [J]. Proc Ncad Sci, 1997, 94:10967-10972
[46]Vierling E. The roles of heat shock proteins in plants [J]. Annu Rev Plant Physiol Plant Mol Biol, 1991, 42:579-620
[47]Dahlgaard J, Loeschcke V P, Michalak J. Induced thermo tolerance and associated expression of the heat shock protein HSP70 in adult Drosophila melanogaster [J]. Functional Ecology, 1998, 12: 786-793
[48] Li G C. Elevated levels of 70 000 dalton heat shock protein in transiently thermo tolerant Chinese hamster fibroblasts and in their stable heat resistant variants [J]. International Journal of Radiation Oncology Biology Physics, 1985, 11: 165-177
[49]王秋香,李宗芸.果蝇热激蛋白的研究进展[J].昆虫知识, 2008,45(4)679-720
[50]许竟成,蔡亚非.动物热激蛋白的研究进展[J].资源开发与市场, 2009, 25 (7) 619-622
[51] Guttman S D, Glover C V, Allis C D, et a1. Heat shock, deciliation and release from anoxia inducethe synthesis of the same polypeptides in starved T pyriformis [J]. Cell, 1980, 22: 299-307
[52] Hahn G M, Li G C. Thermotolerance and heat shock proteins in mammalian cells [J]. Radiation Research, 1982, 92: 452-457
[53] Marber M, Mestril R, Chi S H, et a1. Over expression of the rat inducible 70kDa heat stress protein in a transgenic mouse increases the resistance of the heart to ischemic injury [J]. The Journal of Clinical Investigation, 1995, 95(4): 1446-1456
[54] Weitzel G, Pilatus U, Rensing L. Similar dose response of heat shock protein synthesis and intracellular pH change in yeast [J]. Experimental Cell Research, 1985, 159: 252-256
[55] Bruemmer-Smith S, Stuber F, Schroeder. Protective functions of intracellular heat shock protein (HSP) 70 expression in patients with severe sepsis [J].Intensive Care Med.2001, 27:1835-1841
[56] Duverger O, Paslaru L, Morange M. HSP25 is involved in two steps of the differentiation of PAM212 keratinocytes [J]. J Biol Chem, 2004, 279 (11): 10252-10260
[57] Miyata Y. Molecular chaperone HSP90 as a novel target for cancer chemotherapy [J]. Nippon Yakurgaku Zasshi, 2003, 121(1): 33-42
[58] Bond U, Schlesinger M J. Heat shock proteins and development [J]. Adv Genet. 1987, 24: 1-29
[59] Zimmermann J L, Petri W, Meselson M. Accumulation of a specific subset of D. melanogaster heat shock mRNAs in normal development without heat shock [J]. Cell, 1983, 32: 1161-1170
[60] Wehmeyer N, Hernandez L D, Finkelstein R R, et a1. Plant Physiol. 1996.112, 747-757
[61] Prandl R, Schoffl F. Plant Mol. Biol. 1996, 31, 157-I62.
[62] Yocum G D, Joplin K H, Denlinger D L. Upregulation of a 23kDa small heat shock protein transcript during pupal diapause in the flesh fly, Sarcophaga crassipalpis [J]. Insect Biochemistry and Molecular Biology, 1998, 28, 677-682
[63] Morrison L E, Hoover H E, Thuerauf DJ, et a1. Mimicking phosphorylation of alphaB-crystallin on serine-59 is necessary and sufficient to provide maximal protection of cardiac myocytes from apoptosis [J]. Circ Res, 2003, 92(2):203-211
[64] Ehrnsperger M, Graber S, Gaestel M, et al. Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation [J]. EMBO J, 1997, 16(2):221-229
[65] Arata S, Hamaguchi S, Nose K. Inhibition of colony formation of NIH 3T3 cells by the expression of the small molecular weight heat shock protein HSP27: involvement of its phosphorylation and aggregation at the C-terminal region [J]. J Cell Physiol, 1997, 170(1):19-26
[66] Benndorf R, Hayess K, Ryazantsev S, et al. Phosphorylation and supramolecular organization of murine small heat shock protein HSP25 abolish its actin polymerization-inhibiting activity [J]. J Biol Chem, 1994, 269(32):20780-20784
[67] Preville X, Schultz H, Knauf U, et al. Analysis of the role of Hsp25 phosphorylation reveals the importance of the oligomerization state of this small heat shock protein in its protective function against TNFalpha- and hydrogen peroxide-induced cell death [J]. J Cell Biochem, 1998, 69(4):436-452
[68] Clark JI, Muchowski PJ. Small heat-shock proteins and their potential role in human disease [J]. Curr Opin Struct Biol, 2000, 20(1):52-59
[69] Fan GC, Chu G, Mitton B, et al. Small heat-shock protein Hsp20 phosphorylation inhibits beta-agonist-induced cardiac apoptosis [J]. Circ Res, 2004, 94(11):1474-1482
[70]万永奇,谢维.生命科学与人类疾病研究的重要模型-果蝇[J].生命科学, 2006, 18(5): 425-429
[71] Ciocca,DR., Fuqua,SA, Lock Lin S,et al. Response of human breast cancer cells to heat shock and chemotherapeutic drugs [J]. Cancer Res. 1992, 52:3648-3654
[72] Susek, RE, Lindquist SL. HSP26 of saccharomyces cerevisiac is related to the super family of small heat shock proteins bur is without a demonstrable function [J]. Mol.Cell. 1989, 9 (11):5265-5272
[73] Daohai Zhang, Thomas C. Putti. Over-expression of ERp29 attenuates doxorubicin-induced cell apoptosis through up-regulation of Hsp27 in breast cancer cells [J]. Experimental Cell Research, 2010, 316(20): 3522-3531
[74] Favet N, Duverger O, Loones M T, et a1. OverexDression of murine small heat shock protein HSP25 interferes with chondrocyte differentiation and decreases cell adhesion [J]. Cell Death Differ, 2001, 8(6):603-613
[75]秦焱,王慧,昌增益.线虫中的小分子热休克蛋白HSP12.1具有类分子伴侣活性[J].生物化学与生物物理进展,2007,34(6):620-624
[76] Haass C, Klein U, Kloetzel P M. Developmental expression of Drosophila melanogaster small heat-shock proteins [J]. J Cell Sci, 1990, 96 (Pt 3): 413-418
[77]王佳颖,张景霞,刘箭,等.组成型表达线粒体小分子热激蛋白提高番茄抗冷性.山东师范大学(自然科学版). 2008, 23 (4): 100-102.
[78]肖成峰,陈胜,高雅娟. HSP71在抗癌药物足草乙甙致成人肝细胞凋亡中的作用[J].中华劳动卫生职业病杂志, 2000, 18(2):78-81
[79]宋松.幼苗期小麦热激蛋白的诱导研究[J].安徽农业科学, 2010, 38(2):595-596
[80]张敏,李建成,张青文,等.热激蛋白90与中红侧沟茧蜂的发育关系[J].昆虫知识, 2009, 46 (2): 284-289
[81]童田雨,冯向飞.热激蛋白70在兔动脉粥样硬化易损斑块内的高表达[J].中国临床保健杂志, 2009, 12 (1): 44-48
[82]张作法,时连根.桑枝芸香苷对硫代乙酰胺引起的小鼠急性肝损伤的保护作用[J].中国蚕学会第五届青年学术研讨会论文集,苏州, 2007. 9: 33 - 34
[83]方玲芬.曲克芦丁注射液无菌检查法方法学研究[J].贵阳医学院学报, 2006, 31 (6) : 590 - 592
[84]武征,汪琳,张建华,等.芸香苷对实验性急性胰腺炎胰外多器官损伤的保护作用及机制[J].中国中医基础医学杂志, 2007, 13 (3) : 211 - 215
[85]李家富,何涛,李刚,等.醋柳黄酮单体对高密度脂蛋白氧化修饰的影响[J].现代中西医结合杂志, 2004, 13(24) : 3234 - 3235
[86]赵国屏.生物信息学[M].北京,科学出版社: 2000
[87]王魏杰.生命科学领域的前沿科学:生物信息学[J].河北理工学院学报, 2004, 26(1): 126-128
[88]张成岗,贺福初.生物信息学方法与实践[M],北京,科学出版社, 2002
[89]李斌,夏庆友,鲁成,等.低分子量热激蛋白Bmhsp19.9基因的表达[J].农业生物技术学报, 2005, 13 (2): 195-201
[90]谢敏,贡成良,薛仁宇,等.家蚕hsp20.4启动子克隆及其驱动表达产物EGT对家蚕蛹体发育的影响[J].昆虫学报, 2009, 52 (3) : 246-253
[91]李斌,夏庆友,鲁成.家蚕肾脏形卵(ki)的荧光差异显示研究[J].农业生物技术学报, 2002, 10 (3): 262-266
[92]孙卫忠,李斌,鲁成,等.家蚕HSP20.8基因体外表达及荧光原位杂交研究[J].蚕业科学, 2007, 33 (1):14-19
[93]李斌,夏庆友,鲁成,等.低分子量热激蛋白的进化研究[J].蚕业科学, 2004, 30 (3): 260-265
[94]张静.家蚕热休克蛋白Bmhsp21.4基因的研究[D].浙江理工大学, 2010
[95] Qing Sheng, Jiayin Xia, Yaozhou Zhang, et al. Cloning, Expression, and Cell Localization of a Novel Small Heat Shock Protein Gene: BmHSP25.4 [J]. Appl Biochem Biotechnol, 2010, 162(5): 1297-1305
[96] Basha E, Lee GJ, Breci LA, et al. The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions [J]. J Biol Chem, 2004, 279(9):7566-7575.
[97] Friedrich KL, Giese KC, Buan NR, et al. Interactions between small heat shock protein subunits and substrate in small heat shock protein-substrate complexes [J]. J Biol Chem, 2004, 279(2):1080-1089.
[98] Haslbeck M, Braun N, Stromer T, et al. Hsp42 is the general small heat shock protein in the cytosol of Saccharomyces cerevisiae [J]. EMBO J, 2004, 23(3):638-649
[99] Qazi KR, et a1. Vaccine, 2005, 23(9):1114-1125
[100]王荫长主编.昆虫生物化学[M].北京:中国农业出版社. 2001.
[101] Adnan S, Susan L, David W. Expression of small heat shock proteins at low temperatures [J].Plant Physiologists, 1998, 117(2):651-658
[102] Leshem Y, Kuiper P. Is there a GAS (general adaption syndrome) response to barious types of environmental stress [J].Biol Plant, 1996, 38:118
[103]许仁林,师素云,易琼华.热激处理与水稻雄性不育系育性转变关系的初步研究[J].武汉植物学研究,1992,10(4):297-304
[104]陈建南,傅鸿仪,路子显,等.温度、热激蛋白与高粱育性的变化[J].遗传学报, 1998, 25(4): 356-361
[105] Sanders BM. Stress proteins in aquatic organisms: an environmental perspective [J]. Crit Rev Toxicol, 1993, 23: 49-75.
[106] Huggett R. Enzyme and protein synthesis as indicators of contaminant exposure and effect [J]. Florida: Lewis Boca Raton, 1 992:235-335
[107]培军,熊先哲,杨桂芬,等.动物生物标志物在土壤污染物生态学研究中的应用[J],应用生态学报, 2003, 14(12): 2347-2350
[108] Staempfli C, Beeker-van Slooten K, Tarradellas J. HSP70 instability and induction by a pesticide in Folsomia Candida [J]. Biomarker, 2002, 7(1): 68-79
[109] Queitsch Cetal. HSP90 as a capacitor of phenotypic variation [J]. Nature, 2002, 4 (17): 618-624.
[110]邓家术,段彬江,刘中来.植物热激蛋白的研究进展及应用[J].生命的化学, 2003, 23(3): 226-228.
[111]Laszlo O Jr, Insug O, Mark E R, et al. Interaction between Heat Shock Proteins and Antimicrobial Peptides [J]. Biochemistry 2000, 39, 14150-14159
[112] Lima KM, Santos R R, Brandao I T, et a1. Efficacy of DNA HSP65 vaccination for tuberculosis varies with method of DNA introduction in vivo [J]. Vaccine, 2003, 22(1):49-56.
[113] Manjili M H, Wang X Y, Park J, et a1. Immunotherapy of cancer using heat shock proteins [J]. Front Biosci, 2002, 7:43-52
[114] Higuchi R, Fockler C, Dollinger G, et al. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions [J]. Biotechnology (N Y), 1993, 11(9): 1026-1030
[115]J Sambrook, E F Fritsch, T Maniatis. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989
[116] Yi Zhang, Zhenguo Wei, YuanYuan Li, et al. Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy [J].Analytical Biochemistry, 2009, 394: 202-208
[117]黄惠芳,马飞.热激蛋白的分子进化研究[J].厦门大学学报(自然科学版), 2004, 43: 166-170
[118]李斌.家蚕分子连锁图谱的构建和低分子量热激蛋白基因的克隆与特异表达[D].西南农业大学, 2001
[119] Li Z W, Xiang Z H, Zhang Z, et al. The small heat shock protein (sHSP) genes in the silkworm, Bombyx mori, and comparative analysis with other insect sHSP genes. BMC Evolutionary Biology, 2009, 9:215
[120] Otaka T. Chromosomal action of ecdysone [J]. Nature, 1980, 285(12):435
[121] Catalan RE, Martines A M, Aragones MD. In vitro effect of ecdysterone on protein kinase activity [J]. Comp Biochen Physiol.1982, 71B (2):301
[122] Takei M, Endo K,Nishimmoto N, et al. Effect of ecdysterone on histamine release fro rat peritoneal mast cells [J]. Pharm Sci, 1991,80 (4):309
[123] Catalan RE, Aragones MD, Godoy JE, et al. Ecdysterone induces acetylcholinesterase in mammalian brain [J]. Comp Biochel Physiol, 1984, 78 C (1):193
[124] Manglesdorf D J, Thummel C, Beato M, et al. The nuclear receptor superfamily: the second decade [J]. Cell, 1995, 83: 835 - 839
[125]Henrich V C, Rybczynski R, Gilbert L I. Peptide hormones, steroid hormones, and puffs: Mechanisms and models in insect development [J]. Vitamins and Hormones, 1999, 55: 73-125.
[126]Gilbert LI, Rybczynski R, Warren JT. Control and biochemical nature of the ecdysteroidogenic pathway [J]. Annual Review of Entomology, 2002, 47: 883 - 916.
[127] Gu SH, Chow YS. Analysis of ecdysteroidogenic activity of the prothoracic glands during the last larval instar of the silkworm, Bombyx mori [J]. Arch. Insect Biochem. Physiol, 2005a, 58 (1): 17-26
[128] Gu SH, Chow YS. Temporal changes in DNA synthesis of prothoracic gland cells during larval development and their correlation with ecdysteroidogenic activity in the silkworm, Bombyx mori [J]. J Exp Zoolog A Comp Exp Biol, 2005b, 303 (4): 249-258
[139]郭恩恩,李胜,曹阳, 2008.昆虫蜕皮的激素调控网络[J].蚕业科学, 34(2) : 370-374
[130]路爱成,卫正国,李兵,沈卫德, 2009.野桑蚕不同组织细胞色素P450 CYP305B1V1基因的诱导表达特征研究[J].安徽农业科学. 37(12): 5400-5402
[131]孔岳樵.蚕桑的蜕皮激素[J].蚕桑茶叶通讯,1994(1):36-39
[132]徐世清,徐俊良.蜕皮激素对桑蚕的影响及应用前景[J].江苏蚕业, 1995, 2: 1-4
[133]赵国栋,卫正国,沈卫德,等.家蚕谷胱甘肽-S-转移酶基因(BmGSTd1)的诱导表达定量分析[J].蚕业科学, 2010, 36(1):46-51
[134]卫正国.家蚕细胞色素P450基因及其定量方法的研究[D].苏州:苏州大学, 2009
[135]de Miguel N, Braun N, Bepperling A, et al. Structural and functional diversity in the family of small heat shock proteins from the parasite Toxoplasma gondii [J]. Biochim Biophys Acta, 2009, 1793(11): 1738-1748
[136]Carrasco R, Almoguera C, Jordano J. A plant small heat shock protein gene expressed during zygotic embryogenesis but noninducible by heat stress [J]. J Biol Chem, 1997, 272(43): 27470-27475
[137]王琛柱.植物抗虫性的化学基础[J].植物保护, 1993, 19(6):39-41